Lag0038499 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0038499
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr2: 18765742 .. 18766823 (-)
RNA-Seq Expression	Lag0038499
Synteny	Lag0038499

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCCACCGGGGTTGATGAAGCAGCACTTCCAGATCCTGTAACGCTTCCAGATCCCTTTTCCGCCATGGGAGGAATCGAAGGTGAAAGCTTAGGAGAAGTTGGAAGTTAAAAAACGCGGGAGCGAAAAATTTCGCTCTGATACCATTTCAAGACAGAGAGTAAATAACGATGTTCTCTCGTGAATATTATTCAATAAAGAAGAAGAATATATACAAGGCAATACCAGAGAACTAACTAACTGAATGGAGTTAAATACAAACAATAACTCAACTAACAAGGTTAAGAATAAACTCTGGTTAATATAATCTAACAGACATATCTGTTCCAGAGATTGAAAGAGCTCTTTTTGAAATGAACCCGGAAAAGGCTCCAGGACCGGATGGAATGACAGCTGCTTTTTTCCAAGATAATTGGAAGACCGTACAGAGAGAGGTGATCGATACAGTGAAGTCGTTCTTCATGCATGGGAATGCTGAGGAAGATGAATCACACACACATTGTACTCATTCCGAAGGTGAAACACCCCACTAGAATGGCCCAATTGCGGCCTATAGCATTGTACAACGTTGCGTACAAAATAGTCTCGAAGATCCTCTGTAATAGACTGAAGGGGGTGCTTCCAAAAATTATTAGTGATTGCCAAACAGCGTTTTTCCAGGCCGACTAATATCGGACAATATTCATATTGCACACGAAATACTACATTCGATGAATGGAAAGAGGAGAGGGAACGGGTACACCATGGCGGTAAAGTTAGATATGGCAAAAGCATATGAGCGCATTGAATGGGGCTTTCTTGAAAGGGTGCTCTCAATATTTGGCTTTGACCAAAGATGGATAGGGTGGGTTATGGAATGCATCACATCAGTCTCATATAGTTTGATGGTGAATAATAAGCCGCATGGGTTAATATTACCAGAAAGAGGGCTTCGACAAGGGGACCCGCTATCGCCCTACCTCTTCGTTTTATGTTCAGAAGTTTTCACCTTCTTGCTGCAAAAATCAGCTCTCACTGGCTACGCGGTTTCAAGGAATGGTCCTGCCATATCGAATTTGTTTTCGCTGACGATTCCCTGA

mRNA sequence

ATGGAAGCCACCGGGGTTGATGAAGCAGCACTTCCAGATCCTGTAACGCTTCCAGATCCCTTTTCCGCCATGGGAGGAATCGAAGACATATCTGTTCCAGAGATTGAAAGAGCTCTTTTTGAAATGAACCCGGAAAAGGCTCCAGGACCGGATGGAATGACAGCTGCTTTTTTCCAAGATAATTGGAAGACCGTACAGAGAGAGGTGATCGATACAGTGAAGTCGTTCTTCATGCATGGGAATGCTGAGGAAGATGAATCACACACACATTGTACTCATTCCGAAGGCCGACTAATATCGGACAATATTCATATTGCACACGAAATACTACATTCGATGAATGGAAAGAGGAGAGGGAACGGGTACACCATGGCGGTAAAGTTAGATATGGCAAAAGCATATGAGCGCATTGAATGGGGCTTTCTTGAAAGGGTGCTCTCAATATTTGGCTTTGACCAAAGATGGATAGGGTGGGTTATGGAATGCATCACATCAGTCTCATATAGTTTGATGGTGAATAATAAGCCGCATGGGTTAATATTACCAGAAAGAGGGCTTCGACAAGGGGACCCGCTATCGCCCTACCTCTTCGTTTTATGTTCAGAAGTTTTCACCTTCTTGCTGCAAAAATCAGCTCTCACTGGCTACGCGGTTTCAAGGAATGGTCCTGCCATATCGAATTTGTTTTCGCTGACGATTCCCTGA

Coding sequence (CDS)

ATGGAAGCCACCGGGGTTGATGAAGCAGCACTTCCAGATCCTGTAACGCTTCCAGATCCCTTTTCCGCCATGGGAGGAATCGAAGACATATCTGTTCCAGAGATTGAAAGAGCTCTTTTTGAAATGAACCCGGAAAAGGCTCCAGGACCGGATGGAATGACAGCTGCTTTTTTCCAAGATAATTGGAAGACCGTACAGAGAGAGGTGATCGATACAGTGAAGTCGTTCTTCATGCATGGGAATGCTGAGGAAGATGAATCACACACACATTGTACTCATTCCGAAGGCCGACTAATATCGGACAATATTCATATTGCACACGAAATACTACATTCGATGAATGGAAAGAGGAGAGGGAACGGGTACACCATGGCGGTAAAGTTAGATATGGCAAAAGCATATGAGCGCATTGAATGGGGCTTTCTTGAAAGGGTGCTCTCAATATTTGGCTTTGACCAAAGATGGATAGGGTGGGTTATGGAATGCATCACATCAGTCTCATATAGTTTGATGGTGAATAATAAGCCGCATGGGTTAATATTACCAGAAAGAGGGCTTCGACAAGGGGACCCGCTATCGCCCTACCTCTTCGTTTTATGTTCAGAAGTTTTCACCTTCTTGCTGCAAAAATCAGCTCTCACTGGCTACGCGGTTTCAAGGAATGGTCCTGCCATATCGAATTTGTTTTCGCTGACGATTCCCTGA

Protein sequence

MEATGVDEAALPDPVTLPDPFSAMGGIEDISVPEIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGNAEEDESHTHCTHSEGRLISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQRWIGWVMECITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKSALTGYAVSRNGPAISNLFSLTIP

Homology

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BLAST of Lag0038499 vs. NCBI nr
Match: KAG5561220.1 (hypothetical protein RHGRI_004294 [Rhododendron griersonianum])

HSP 1 Score: 199.9 bits (507), Expect = 2.4e-47
Identity = 103/256 (40.23%), Postives = 139/256 (54.30%), Query Frame = 0


Query: 27  IEDISVPEIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGNAEEDE 86
           I  I + E++ ALF+M P  APGPDGMT  FFQ  W  V RE+ D ++SF   G   +  
Sbjct: 101 IRPIFIGEVKTALFQMKPSTAPGPDGMTVGFFQKYWDVVGREITDALRSFMFSGKLLKGM 160

Query: 87  SHTH-------------------------------------------------CTHSEGR 146
           +HTH                                                 C    GR
Sbjct: 161 NHTHIVLIPKVKCPVNMTQLRPISLCNVAYKILAKVLANRLSKVLPAVVSPNQCAFVPGR 220

Query: 147 LISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQRWIG 206
           +I+DNI I  EI H +  KR G  + +AVKLDM+KAY+R+EW FLER++   GF  +W+G
Sbjct: 221 VITDNILIGQEIFHHLKNKRGGRCHNVAVKLDMSKAYDRVEWIFLERIMLKLGFHLKWVG 280

Query: 207 WVMECITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKS----AL 230
           WVMEC+ +VSYS +++ +  G I P RGLRQGDPLSP+LF+LC E  + +LQK     ++
Sbjct: 281 WVMECLKTVSYSFLLDGQISGYIKPSRGLRQGDPLSPFLFLLCGEGLSVMLQKGEMERSI 340

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BLAST of Lag0038499 vs. NCBI nr
Match: KAG5561219.1 (hypothetical protein RHGRI_004294 [Rhododendron griersonianum])

HSP 1 Score: 199.9 bits (507), Expect = 2.4e-47
Identity = 103/256 (40.23%), Postives = 139/256 (54.30%), Query Frame = 0


Query: 27  IEDISVPEIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGNAEEDE 86
           I  I + E++ ALF+M P  APGPDGMT  FFQ  W  V RE+ D ++SF   G   +  
Sbjct: 428 IRPIFIGEVKTALFQMKPSTAPGPDGMTVGFFQKYWDVVGREITDALRSFMFSGKLLKGM 487

Query: 87  SHTH-------------------------------------------------CTHSEGR 146
           +HTH                                                 C    GR
Sbjct: 488 NHTHIVLIPKVKCPVNMTQLRPISLCNVAYKILAKVLANRLSKVLPAVVSPNQCAFVPGR 547

Query: 147 LISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQRWIG 206
           +I+DNI I  EI H +  KR G  + +AVKLDM+KAY+R+EW FLER++   GF  +W+G
Sbjct: 548 VITDNILIGQEIFHHLKNKRGGRCHNVAVKLDMSKAYDRVEWIFLERIMLKLGFHLKWVG 607

Query: 207 WVMECITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKS----AL 230
           WVMEC+ +VSYS +++ +  G I P RGLRQGDPLSP+LF+LC E  + +LQK     ++
Sbjct: 608 WVMECLKTVSYSFLLDGQISGYIKPSRGLRQGDPLSPFLFLLCGEGLSVMLQKGEMERSI 667

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BLAST of Lag0038499 vs. NCBI nr
Match: KAG5561218.1 (hypothetical protein RHGRI_004294 [Rhododendron griersonianum])

HSP 1 Score: 199.9 bits (507), Expect = 2.4e-47
Identity = 103/256 (40.23%), Postives = 139/256 (54.30%), Query Frame = 0


Query: 27  IEDISVPEIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGNAEEDE 86
           I  I + E++ ALF+M P  APGPDGMT  FFQ  W  V RE+ D ++SF   G   +  
Sbjct: 302 IRPIFIGEVKTALFQMKPSTAPGPDGMTVGFFQKYWDVVGREITDALRSFMFSGKLLKGM 361

Query: 87  SHTH-------------------------------------------------CTHSEGR 146
           +HTH                                                 C    GR
Sbjct: 362 NHTHIVLIPKVKCPVNMTQLRPISLCNVAYKILAKVLANRLSKVLPAVVSPNQCAFVPGR 421

Query: 147 LISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQRWIG 206
           +I+DNI I  EI H +  KR G  + +AVKLDM+KAY+R+EW FLER++   GF  +W+G
Sbjct: 422 VITDNILIGQEIFHHLKNKRGGRCHNVAVKLDMSKAYDRVEWIFLERIMLKLGFHLKWVG 481

Query: 207 WVMECITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKS----AL 230
           WVMEC+ +VSYS +++ +  G I P RGLRQGDPLSP+LF+LC E  + +LQK     ++
Sbjct: 482 WVMECLKTVSYSFLLDGQISGYIKPSRGLRQGDPLSPFLFLLCGEGLSVMLQKGEMERSI 541

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BLAST of Lag0038499 vs. NCBI nr
Match: KAG5561221.1 (hypothetical protein RHGRI_004294 [Rhododendron griersonianum])

HSP 1 Score: 199.9 bits (507), Expect = 2.4e-47
Identity = 103/256 (40.23%), Postives = 139/256 (54.30%), Query Frame = 0


Query: 27  IEDISVPEIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGNAEEDE 86
           I  I + E++ ALF+M P  APGPDGMT  FFQ  W  V RE+ D ++SF   G   +  
Sbjct: 49  IRPIFIGEVKTALFQMKPSTAPGPDGMTVGFFQKYWDVVGREITDALRSFMFSGKLLKGM 108

Query: 87  SHTH-------------------------------------------------CTHSEGR 146
           +HTH                                                 C    GR
Sbjct: 109 NHTHIVLIPKVKCPVNMTQLRPISLCNVAYKILAKVLANRLSKVLPAVVSPNQCAFVPGR 168

Query: 147 LISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQRWIG 206
           +I+DNI I  EI H +  KR G  + +AVKLDM+KAY+R+EW FLER++   GF  +W+G
Sbjct: 169 VITDNILIGQEIFHHLKNKRGGRCHNVAVKLDMSKAYDRVEWIFLERIMLKLGFHLKWVG 228

Query: 207 WVMECITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKS----AL 230
           WVMEC+ +VSYS +++ +  G I P RGLRQGDPLSP+LF+LC E  + +LQK     ++
Sbjct: 229 WVMECLKTVSYSFLLDGQISGYIKPSRGLRQGDPLSPFLFLLCGEGLSVMLQKGEMERSI 288

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BLAST of Lag0038499 vs. NCBI nr
Match: XP_019091159.1 (PREDICTED: uncharacterized protein LOC109128743 [Camelina sativa])

HSP 1 Score: 199.1 bits (505), Expect = 4.1e-47
Identity = 99/221 (44.80%), Postives = 135/221 (61.09%), Query Frame = 0


Query: 34  EIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGNAEEDESHTH--- 93
           EI +ALF M+P+KAPGPDGMTA FFQ  W  V+ +++  V  F   G+ E+  + TH   
Sbjct: 322 EIRKALFMMHPDKAPGPDGMTALFFQKAWTVVKTDLVSMVNQFLEEGDFEKGLNRTHICL 381

Query: 94  -------------------CTHSEGRLISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMA 153
                                   GRLISDNI IA E+ H +   R   G  MA+K DM+
Sbjct: 382 IPKVVKPTRMTELRPIKTQTAFVPGRLISDNILIAQEMFHGLRTNRSCRGKFMAIKTDMS 441

Query: 154 KAYERIEWGFLERVLSIFGFDQRWIGWVMECITSVSYSLMVNNKPHGLILPERGLRQGDP 213
           KAY+R+EWGFL+ +    GF +RWI W+M C++SV Y +++N +P+GLI+PERGLRQGDP
Sbjct: 442 KAYDRVEWGFLKAMFRKMGFSERWISWIMFCVSSVEYRVLLNGQPNGLIVPERGLRQGDP 501

Query: 214 LSPYLFVLCSEVFTFLLQKS----ALTGYAVSRNGPAISNL 229
           LSPYLF+LC+EV    +QK+     + G  V+   P I++L
Sbjct: 502 LSPYLFILCTEVLIANIQKAEAVKRIMGIKVANKCPPITHL 542

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BLAST of Lag0038499 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 6.5e-11
Identity = 32/62 (51.61%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 171 MVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKS----ALTGYAVSRNGPAIS 229
           ++N  P GL+ P RGLRQGDPLSPYLF+LC+EV + L +++     L G  VS N P I+
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

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BLAST of Lag0038499 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-09
Identity = 60/240 (25.00%), Postives = 95/240 (39.58%), Query Frame = 0


Query: 30  ISVPEIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGN-------- 89
           I++ E+ +AL  M   K+PG DG+T  FFQ  W T+  +    +   F  G         
Sbjct: 449 ITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLGPDFHRVLTEAFKKGELPLSCRRA 508

Query: 90  ---------------------------------------------AEEDESHTHCTHSEG 149
                                                           D+S+T      G
Sbjct: 509 VLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYT----VPG 568

Query: 150 RLISDNIHIAHEILHSMNGKRRGNGYTMA-VKLDMAKAYERIEWGFLERVLSIFGFDQRW 209
           R I DN+ +  ++LH      R  G ++A + LD  KA++R++  +L   L  + F  ++
Sbjct: 569 RTIFDNVFLIRDLLHF----ARRTGLSLAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQF 628

Query: 210 IGWVMECITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKSALTG 216
           +G++     S    + +N      +   RG+RQG PLS  L+ L  E F  LL+K  LTG
Sbjct: 629 VGYLKTMYASAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIEPFLCLLRK-RLTG 679

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BLAST of Lag0038499 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 2.8e-06
Identity = 36/128 (28.12%), Postives = 62/128 (48.44%), Query Frame = 0


Query: 102 NIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQRWIGWVME 161
           NI  +  ++H +N  +  N   M + LD  KA+++I+  F+ +VL   G    ++  +  
Sbjct: 582 NIRKSINVIHYINKLKDKN--HMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIKA 641

Query: 162 CITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFT-FLLQKSALTGYAVSR 221
             +    ++ VN +    I  + G RQG PLSPYLF +  EV    + Q+  + G  + +
Sbjct: 642 IYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQKEIKGIQIGK 701

Query: 222 NGPAISNL 229
               IS L
Sbjct: 702 EEVKISLL 707

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BLAST of Lag0038499 vs. ExPASy Swiss-Prot
Match: Q05118 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 6.3e-06
Identity = 31/93 (33.33%), Postives = 48/93 (51.61%), Query Frame = 0


Query: 117 RRGNGYTM-AVKLDMAKAYERIEWGFLERVLSIFGFDQRWIGWVMECITSVSYSLMVNNK 176
           RR  G T   V LD+ KA++ +    + R +  FG D     ++M  IT    +++V  +
Sbjct: 16  RRLKGKTYNVVSLDIRKAFDTVSHPAILRAMRAFGIDDGMQDFIMSTITDAYTNIVVGGR 75

Query: 177 PHGLILPERGLRQGDPLSPYLF-VLCSEVFTFL 208
               I    G++QGDPLSP LF ++  E+ T L
Sbjct: 76  TTNKIYIRNGVKQGDPLSPVLFNIVLDELVTRL 108

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BLAST of Lag0038499 vs. ExPASy TrEMBL
Match: A0A2N9HWM9 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS46209 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 7.6e-47
Identity = 106/259 (40.93%), Postives = 145/259 (55.98%), Query Frame = 0


Query: 28  EDISVP----EIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGNAE 87
           ED+ +P    E+  ALF+M+P KAPGPDGMTA FFQ  W  V  +V D++  F  +G+  
Sbjct: 569 EDLMMPFTREEVRCALFQMSPSKAPGPDGMTALFFQKFWHIVGMDVTDSILDFLNNGHML 628

Query: 88  EDESHTH-------------------------------------------------CTHS 147
           +  ++TH                                                     
Sbjct: 629 KSLNYTHIALIPKVKSPELMTQFRPISLCNVLYKIISKVLVNRMKTILPFVVSDSQSAFV 688

Query: 148 EGRLISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQR 207
            GR+ISDNI IA EILH +  KR G    MAVKLDM+KAY+R+EW +L++++   GF  R
Sbjct: 689 PGRMISDNIMIAFEILHHLKNKRVGKVPQMAVKLDMSKAYDRVEWDYLKKMMLKLGFTAR 748

Query: 208 WIGWVMECITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQK---- 230
           W+  +MEC+TSVSYS++VN +P G + P RGLRQGDPLSPYLF++C+E  T LL+K    
Sbjct: 749 WVALIMECVTSVSYSILVNGEPKGYVKPSRGLRQGDPLSPYLFLICAEGLTALLRKAERE 808

[Close]

BLAST of Lag0038499 vs. ExPASy TrEMBL
Match: A0A2N9FT83 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS21809 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 3.8e-46
Identity = 96/200 (48.00%), Postives = 129/200 (64.50%), Query Frame = 0


Query: 34   EIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGNAEEDESHTHCTH 93
            E+  AL +M P KAPGPDGM   F+Q  W T+ REV+  V S           S T    
Sbjct: 1469 EVRVALKQMAPLKAPGPDGMPPVFYQSYWNTIGREVVQAVLSSI--------NSETQSAF 1528

Query: 94   SEGRLISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQ 153
              GRLISDN+ IA E LH M+ +R G   +M +KLDM+KAY+R+EW FL +V+   GF  
Sbjct: 1529 VPGRLISDNVLIAFETLHHMHNQRTGKNGSMTLKLDMSKAYDRVEWDFLRQVMIKMGFHS 1588

Query: 154  RWIGWVMECITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKSA- 213
            +W+  +MECIT+VSYSL++N +P G I P RGLRQGDP+SPYLF++C+E    LL K+A 
Sbjct: 1589 QWVSLIMECITTVSYSLLINGEPTGHITPSRGLRQGDPISPYLFLICAEGLNGLLNKAAS 1648

Query: 214  ---LTGYAVSRNGPAISNLF 230
               + G ++SR  P +++LF
Sbjct: 1649 QGEIHGVSISRRSPKLTHLF 1660

[Close]

BLAST of Lag0038499 vs. ExPASy TrEMBL
Match: A0A6D2K102 (RNase H domain-containing protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS32969 PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 4.9e-46
Identity = 90/208 (43.27%), Postives = 130/208 (62.50%), Query Frame = 0


Query: 34  EIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFF---------MHGNAEE 93
           E+  A+F ++P+KAPGPDG +A+FF  NW+TV +++I  V+ FF         +H    E
Sbjct: 232 EVSAAIFSIHPDKAPGPDGFSASFFHSNWETVGKDIIQEVQLFFTSGILPRARLHPILNE 291

Query: 94  DESHTHCTHSEGRLISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLER 153
             S        GR ISDN+ I HEILH +   +      MA+K DM KAY+R+EW F++ 
Sbjct: 292 LVSENQSAFVPGRAISDNVMITHEILHFLKISKAKKRGAMAIKTDMTKAYDRVEWDFIKL 351

Query: 154 VLSIFGFDQRWIGWVMECITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVF 213
           VL   GF  +WIGW+M+C+TSV++  ++N    G + P RG+RQGDPLSPYLF+LCSEV 
Sbjct: 352 VLERLGFHTKWIGWIMQCVTSVTFQFLINGTAVGFVQPSRGIRQGDPLSPYLFILCSEVL 411

Query: 214 TFLLQKS----ALTGYAVSRNGPAISNL 229
           + L  K+     +TG  ++  GP +++L
Sbjct: 412 SGLCHKAQANGQITGVRIATRGPQVNHL 439

[Close]

BLAST of Lag0038499 vs. ExPASy TrEMBL
Match: A0A2N9H680 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS35287 PE=4 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 6.4e-46
Identity = 105/256 (41.02%), Postives = 140/256 (54.69%), Query Frame = 0


Query: 27  IEDISVPEIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHG------ 86
           ++D+S  EI  ALF+M P KAPGPDGMTA FFQ  W  V  +V   ++ FF  G      
Sbjct: 210 LKDLSSEEIHSALFQMGPSKAPGPDGMTALFFQKYWHIVGEDVSLAIQDFFHSGRMLGSI 269

Query: 87  ------------NAE-------------------------------EDESHTHCTHSEGR 146
                       N E                               +  S + C    GR
Sbjct: 270 NFTNIVLIPKVKNPEFMSQFRPISLCNVLYKIASKVLVNRMKVILPKVISDSQCAFVPGR 329

Query: 147 LISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQRWIG 206
           LISDN+ +A E+LH +     G  Y MAVKLDM+KAY+R+EW FL+ +L   GF +RW+ 
Sbjct: 330 LISDNVIMAFEVLHYLKNLGTGENYQMAVKLDMSKAYDRVEWNFLQAILFKLGFHRRWVD 389

Query: 207 WVMECITSVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKS----AL 230
            +M C+T+ SY++MVN  PHG I P RGLRQGDPLSPYLF+LC+E  + L++K+     +
Sbjct: 390 LIMTCVTTTSYAVMVNGVPHGYIKPTRGLRQGDPLSPYLFLLCAEGLSALIRKAEREKKI 449

[Close]

BLAST of Lag0038499 vs. ExPASy TrEMBL
Match: A0A2N9J3U0 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS59177 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 8.4e-46
Identity = 105/249 (42.17%), Postives = 137/249 (55.02%), Query Frame = 0


Query: 34   EIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGNAEEDESHTHCT- 93
            EI  ALF+M+P KAPGPDGM A F+Q  W  V  +V + V  F   G   +  + TH T 
Sbjct: 922  EIRSALFQMHPTKAPGPDGMNAMFYQKFWHIVGDDVTNAVLEFLHSGKMLKSVNFTHITL 981

Query: 94   ------------------------------------------------HSEGRLISDNIH 153
                                                               GRLI+DNI 
Sbjct: 982  IPKIPSPELMTQFRPISLCNVLYKIISKVLANRLKTVLDHIISDNQSAFVPGRLITDNIL 1041

Query: 154  IAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQRWIGWVMECIT 213
            +A E LH M  KR+G    MAVKLDM+KAY+R+EW FLE ++   GFDQRW+  +M+C+T
Sbjct: 1042 VAFEALHYMKTKRKGRSTHMAVKLDMSKAYDRVEWNFLENMMMKLGFDQRWVNLIMQCLT 1101

Query: 214  SVSYSLMVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKS----ALTGYAVSR 230
            SVSYS+M+N +P G I P RG+RQGDPLSPYLF++C+E  T LLQ++     + G ++ R
Sbjct: 1102 SVSYSVMLNGEPTGYIKPTRGIRQGDPLSPYLFLICAEGLTALLQQAERSGIVQGLSICR 1161

[Close]

BLAST of Lag0038499 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 69.3 bits (168), Expect = 4.6e-12
Identity = 32/62 (51.61%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 171 MVNNKPHGLILPERGLRQGDPLSPYLFVLCSEVFTFLLQKS----ALTGYAVSRNGPAIS 229
           ++N  P GL+ P RGLRQGDPLSPYLF+LC+EV + L +++     L G  VS N P I+
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

[Close]

BLAST of Lag0038499 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 55.1 bits (131), Expect = 9.0e-08
Identity = 27/61 (44.26%), Postives = 38/61 (62.30%), Query Frame = 0


Query: 96  GRLISDNIHIAHEILHSMNGKRRGNGYTMAVKLDMAKAYERIEWGFLERVLSIFGFDQRW 155
           GR+ +DNI    E +HSM  K+   G+ M +KLD+ KAY+RI W +LE  L   GF + W
Sbjct: 23  GRVSTDNIVFVQEAVHSMRRKKGVKGW-MLLKLDLEKAYDRIRWDYLEDTLISAGFPEVW 82

Query: 156 I 157
           +
Sbjct: 83  L 82

[Close]

BLAST of Lag0038499 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 8.4e-06
Identity = 23/48 (47.92%), Postives = 28/48 (58.33%), Query Frame = 0


Query: 34  EIERALFEMNPEKAPGPDGMTAAFFQDNWKTVQREVIDTVKSFFMHGN 82
           EI  A+F M   KAPGPD  TA FF ++W  V+   I  VK FF  G+
Sbjct: 535 EITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVKEFFRTGH 582

The following BLAST results are available for this feature:

BLAST of Lag0038499 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG5561220.1	2.4e-47	40.23	hypothetical protein RHGRI_004294 [Rhododendron griersonianum]	[more]
KAG5561219.1	2.4e-47	40.23	hypothetical protein RHGRI_004294 [Rhododendron griersonianum]	[more]
KAG5561218.1	2.4e-47	40.23	hypothetical protein RHGRI_004294 [Rhododendron griersonianum]	[more]
KAG5561221.1	2.4e-47	40.23	hypothetical protein RHGRI_004294 [Rhododendron griersonianum]	[more]
XP_019091159.1	4.1e-47	44.80	PREDICTED: uncharacterized protein LOC109128743 [Camelina sativa]	[more]

BLAST of Lag0038499 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P92555	6.5e-11	51.61	Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ...	[more]
P14381	1.6e-09	25.00	Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV...	[more]
P11369	2.8e-06	28.13	LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE...	[more]
Q05118	6.3e-06	33.33	Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra...	[more]

BLAST of Lag0038499 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2N9HWM9	7.6e-47	40.93	Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F...	[more]
A0A2N9FT83	3.8e-46	48.00	Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F...	[more]
A0A6D2K102	4.9e-46	43.27	RNase H domain-containing protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_L...	[more]
A0A2N9H680	6.4e-46	41.02	Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F...	[more]
A0A2N9J3U0	8.4e-46	42.17	Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F...	[more]

BLAST of Lag0038499 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG01250.1	4.6e-12	51.61	RNA-directed DNA polymerase (reverse transcriptase)	[more]
AT4G20520.1	9.0e-08	44.26	RNA binding;RNA-directed DNA polymerases	[more]
AT1G43760.1	8.4e-06	47.92	DNAse I-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 63..212 e-value: 2.1E-17 score: 63.4
None	No IPR available	PANTHER	PTHR19446	REVERSE TRANSCRIPTASES	coord: 29..72 coord: 96..194
None	No IPR available	PANTHER	PTHR19446:SF440	SUBFAMILY NOT NAMED	coord: 29..72 coord: 96..194
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 38..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0038499.1	Lag0038499.1	mRNA