Lag0035709 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0035709
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCalcium-binding EF hand family protein
Locationchr3: 28066635 .. 28067012 (+)
RNA-Seq ExpressionLag0035709
SyntenyLag0035709
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGTGGCTTTTCTCAATGATACAACACTGACAAATTTCATCAACGATACAAAGATGTTTGACGATTGCATCAAGGAGAGCTTTAAGAAGCTTGACGTGGACGGGGATGGCTTCCTGAATGCCGATGAACTCGGTGTCGAGTTTTGCAATCAACCATTAGATCCGATGAAAGATATCTATGAAGTTGTTTGTCATAAGTTTGAAGTGGACAAAAATGGTGGAATTGATGTAAAGGGGTTTATGTCAATAATAAGGGACATTCTACTTGCCATGGCTCAAGGGATTGGAAACTTGCCAATCCAAGTTGCACTTCAAGAAGATAGTTTTCTCACCAAGGCTGTTGAGCATGAAAAGAAGAAGAAAGCAGAAAAATAG

mRNA sequence

ATGAGTGTGGCTTTTCTCAATGATACAACACTGACAAATTTCATCAACGATACAAAGATGTTTGACGATTGCATCAAGGAGAGCTTTAAGAAGCTTGACGTGGACGGGGATGGCTTCCTGAATGCCGATGAACTCGGTGTCGAGTTTTGCAATCAACCATTAGATCCGATGAAAGATATCTATGAAGTTGTTTGTCATAAGTTTGAAGTGGACAAAAATGGTGGAATTGATGTAAAGGGGTTTATGTCAATAATAAGGGACATTCTACTTGCCATGGCTCAAGGGATTGGAAACTTGCCAATCCAAGTTGCACTTCAAGAAGATAGTTTTCTCACCAAGGCTGTTGAGCATGAAAAGAAGAAGAAAGCAGAAAAATAG

Coding sequence (CDS)

ATGAGTGTGGCTTTTCTCAATGATACAACACTGACAAATTTCATCAACGATACAAAGATGTTTGACGATTGCATCAAGGAGAGCTTTAAGAAGCTTGACGTGGACGGGGATGGCTTCCTGAATGCCGATGAACTCGGTGTCGAGTTTTGCAATCAACCATTAGATCCGATGAAAGATATCTATGAAGTTGTTTGTCATAAGTTTGAAGTGGACAAAAATGGTGGAATTGATGTAAAGGGGTTTATGTCAATAATAAGGGACATTCTACTTGCCATGGCTCAAGGGATTGGAAACTTGCCAATCCAAGTTGCACTTCAAGAAGATAGTTTTCTCACCAAGGCTGTTGAGCATGAAAAGAAGAAGAAAGCAGAAAAATAG

Protein sequence

MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLPIQVALQEDSFLTKAVEHEKKKKAEK
Homology
BLAST of Lag0035709 vs. NCBI nr
Match: XP_004142569.1 (uncharacterized protein LOC101222885 [Cucumis sativus] >KGN66803.1 hypothetical protein Csa_006967 [Cucumis sativus])

HSP 1 Score: 179.1 bits (453), Expect = 2.4e-41
Identity = 90/127 (70.87%), Postives = 106/127 (83.46%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----D 60
           MSVAFLNDTT+TNFINDTK+FDDC+KESFKKLD D DGFLN +EL V F + PL     D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDTDNDGFLNMNELRVGFRSHPLEFELMD 60

Query: 61  PMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLPIQVALQEDSFLTKAV 120
           P+ D+ EVVCHKF+V+K+GGID + F S+IRDILLAMAQGIGN P+QVALQ+DSFL KAV
Sbjct: 61  PVDDLSEVVCHKFQVEKSGGIDEEEFKSVIRDILLAMAQGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHEKKKK 123
           E EK ++
Sbjct: 121 ELEKARE 127

BLAST of Lag0035709 vs. NCBI nr
Match: XP_008462771.1 (PREDICTED: uncharacterized protein LOC103501058 [Cucumis melo] >KAA0062591.1 calcium-binding EF hand family protein [Cucumis melo var. makuwa])

HSP 1 Score: 176.4 bits (446), Expect = 1.5e-40
Identity = 91/127 (71.65%), Postives = 104/127 (81.89%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----D 60
           MSVAFLNDTT+TNFINDTK+FDDC+KESFKKLD D DGFLN +EL   F +QPL     D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLPIQVALQEDSFLTKAV 120
           P+ D+ EVVCHKF V+K+GGID K F S+IRDILLAMA GIGN P+QVALQ+DSFL KAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDEKEFKSVIRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHEKKKK 123
           E EK K+
Sbjct: 121 ELEKAKE 127

BLAST of Lag0035709 vs. NCBI nr
Match: TYJ95513.1 (calcium-binding EF hand family protein [Cucumis melo var. makuwa])

HSP 1 Score: 174.1 bits (440), Expect = 7.6e-40
Identity = 89/127 (70.08%), Postives = 103/127 (81.10%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPL-----D 60
           MSVAFLNDTT+TNFINDTK+FDDC+KESFKKLD D DGFLN +EL   F +QPL     D
Sbjct: 1   MSVAFLNDTTVTNFINDTKIFDDCVKESFKKLDRDNDGFLNKNELRNGFGSQPLELELMD 60

Query: 61  PMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLPIQVALQEDSFLTKAV 120
           P+ D+ EVVCHKF V+K+GGID   F S++RDILLAMA GIGN P+QVALQ+DSFL KAV
Sbjct: 61  PVDDLCEVVCHKFNVEKSGGIDENEFKSVVRDILLAMALGIGNFPLQVALQQDSFLMKAV 120

Query: 121 EHEKKKK 123
           E EK K+
Sbjct: 121 ELEKAKE 127

BLAST of Lag0035709 vs. NCBI nr
Match: XP_022158640.1 (uncharacterized protein LOC111025096 [Momordica charantia])

HSP 1 Score: 151.0 bits (380), Expect = 6.9e-33
Identity = 74/122 (60.66%), Postives = 96/122 (78.69%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDI 60
           MSVAFLNDTT+ +FINDTK+FD+C+KESF+KLD+D DG LNA+EL   F     DP+ D+
Sbjct: 1   MSVAFLNDTTVIDFINDTKIFDNCVKESFQKLDIDKDGILNANELLAGF-RSSTDPVDDL 60

Query: 61  YEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLPIQVALQEDSFLTKAVEHEKK 120
            + VC KF V+K+GGI+   F S++ +ILLA+A GIGNLP+QVALQ+D  L KAVEHE+ 
Sbjct: 61  SQTVCRKFNVEKSGGINENEFKSVVTEILLAIAYGIGNLPLQVALQQDGLLMKAVEHERA 120

Query: 121 KK 123
           K+
Sbjct: 121 KE 121

BLAST of Lag0035709 vs. NCBI nr
Match: XP_018805702.1 (uncharacterized protein LOC108979477 [Juglans regia] >KAF5453860.1 hypothetical protein F2P56_023577 [Juglans regia])

HSP 1 Score: 114.8 bits (286), Expect = 5.5e-22
Identity = 57/127 (44.88%), Postives = 86/127 (67.72%), Query Frame = 0

Query: 1   MSVAFLNDTTLTNFINDTKMFDDCIKESFKKLDVDGDGFLNADELGVEF---------CN 60
           MSVA +N +T+  F+ D K F+ CI+E F+ LD+DG+G L+  EL  EF          +
Sbjct: 1   MSVAMVNSSTIMGFVKDRKTFNSCIEECFEMLDIDGNGTLSRKELRGEFHRVLSLGYYKS 60

Query: 61  QPLDPMKDIYEVVCHKFEVDKNGGIDVKGFMSIIRDILLAMAQGIGNLPIQVALQEDSFL 119
           +P +   D++E+V  +F+ DKNG ID K FM++IR+++ AMA+GIG+ P+ VAL++DS L
Sbjct: 61  EPEEETNDLHEIVFKRFDEDKNGVIDRKEFMALIRELMQAMARGIGDSPVLVALEQDSLL 120

BLAST of Lag0035709 vs. ExPASy Swiss-Prot
Match: Q9BXU9 (Calcium-binding protein 8 OS=Homo sapiens OX=9606 GN=CALN1 PE=1 SV=2)

HSP 1 Score: 44.7 bits (104), Expect = 9.1e-04
Identity = 20/63 (31.75%), Postives = 38/63 (60.32%), Query Frame = 0

Query: 23  DCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFM 82
           D I+E+F+ LD DG+GF++  ELG+   +    P +    ++  + ++D +G +D   FM
Sbjct: 81  DEIREAFRVLDRDGNGFISKQELGMAMRSLGYMPSEVELAIIMQRLDMDGDGQVDFDEFM 140

Query: 83  SII 86
           +I+
Sbjct: 141 TIL 143

BLAST of Lag0035709 vs. ExPASy Swiss-Prot
Match: Q9JJG7 (Calcium-binding protein 8 OS=Mus musculus OX=10090 GN=Caln1 PE=2 SV=2)

HSP 1 Score: 44.7 bits (104), Expect = 9.1e-04
Identity = 20/63 (31.75%), Postives = 38/63 (60.32%), Query Frame = 0

Query: 23  DCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFM 82
           D I+E+F+ LD DG+GF++  ELG+   +    P +    ++  + ++D +G +D   FM
Sbjct: 81  DEIREAFRVLDRDGNGFISKQELGMAMRSLGYMPSEVELAIIMQRLDMDGDGQVDFDEFM 140

Query: 83  SII 86
           +I+
Sbjct: 141 TIL 143

BLAST of Lag0035709 vs. ExPASy Swiss-Prot
Match: Q06BI3 (Calcium-binding protein 8 OS=Rattus norvegicus OX=10116 GN=Caln1 PE=1 SV=2)

HSP 1 Score: 44.7 bits (104), Expect = 9.1e-04
Identity = 20/63 (31.75%), Postives = 38/63 (60.32%), Query Frame = 0

Query: 23  DCIKESFKKLDVDGDGFLNADELGVEFCNQPLDPMKDIYEVVCHKFEVDKNGGIDVKGFM 82
           D I+E+F+ LD DG+GF++  ELG+   +    P +    ++  + ++D +G +D   FM
Sbjct: 81  DEIREAFRVLDRDGNGFISKQELGMAMRSLGYMPSEVELAIIMQRLDMDGDGQVDFDEFM 140

Query: 83  SII 86
           +I+
Sbjct: 141 TIL 143

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004142569.12.4e-4170.87uncharacterized protein LOC101222885 [Cucumis sativus] >KGN66803.1 hypothetical ... [more]
XP_008462771.11.5e-4071.65PREDICTED: uncharacterized protein LOC103501058 [Cucumis melo] >KAA0062591.1 cal... [more]
TYJ95513.17.6e-4070.08calcium-binding EF hand family protein [Cucumis melo var. makuwa][more]
XP_022158640.16.9e-3360.66uncharacterized protein LOC111025096 [Momordica charantia][more]
XP_018805702.15.5e-2244.88uncharacterized protein LOC108979477 [Juglans regia] >KAF5453860.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
Q9BXU99.1e-0431.75Calcium-binding protein 8 OS=Homo sapiens OX=9606 GN=CALN1 PE=1 SV=2[more]
Q9JJG79.1e-0431.75Calcium-binding protein 8 OS=Mus musculus OX=10090 GN=Caln1 PE=2 SV=2[more]
Q06BI39.1e-0431.75Calcium-binding protein 8 OS=Rattus norvegicus OX=10116 GN=Caln1 PE=1 SV=2[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.238.10coord: 4..99
e-value: 1.3E-8
score: 36.9
NoneNo IPR availablePANTHERPTHR34574:SF12CALCIUM-BINDING EF HAND FAMILY PROTEINcoord: 1..121
NoneNo IPR availablePANTHERPTHR34574CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATEDcoord: 1..121
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 33..45
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 20..55
score: 10.189618
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 14..97

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0035709.1Lag0035709.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding