Lag0034511 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0034511
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionIAA-amino acid hydrolase ILR1-like 4
Locationchr3: 8015090 .. 8019414 (-)
RNA-Seq ExpressionLag0034511
SyntenyLag0034511
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCTACACAGTTGGCTGATTTTGATTTTCATTTTCCATGTGCTTATGCTATGGCCAATTCCTTCGACTTCTTCTCCGAGCCCAGAAGAGTTGTCTCAAATCCCTTTGAATTTCCTTCAACATGCTAAAAATCCAGAGGTTTTTGATTGGATGGTTGATATCAGAAGGAAAATACACGAGAATCCTGAACTGGGTTTCGAGGAATTTGAGACCAGCAAGCTTATCAGAGCGGAGCTTGACCGTTTAGGCATTTCTTATAGATACCCAGTGGCCATCACCGGCGTGGTTGGTTTCATTGGGACTGGAAACCCTCCTTTCGTTGCTATTCGGGCAGATATGGATGCGCTAGCCCTGCAGGTACACTTTGTAGCCAAATCGTTTGATCCCCTTATTTCTTTCTGCTTATTTTGGTAATTGTATGGATGAATTTGAAATTGAGGATTACTTCAACTGGTATATTCCGAATGGATTTCACCCATTGTTGCATCTAAATGCCATGTTATGAAACTTAGGGAGAAAATTTCTTGAAATATAATGGAGTTTTGGTGGGTGGATGTATGTATTGTAGGAGGGCGTAGAATGGGCACACAAAAGCAAAGTTCCTGGAAAGATGCATGCGTGTGGACATGATGCTCATGTTGCCATGCTTCTAGGGGCTGCAAAGATTCTTCAAGAGCACCGCCGTCTAATACAGGTTCTTCCGCTTGAAACTTGTGTGGTTGAATATTGATATGGAGATTACATTTTTAAGTTTGTTTTTTTCATTCTAACTCGGAGATTTAAGAGTTGTCACATACATTATACCTATTATTCTTATTGATTTCGGTTGTTTTGAAATCCCTAAGTTCACTCTATTGAGAATGCGACCAAAGTCCTACATTGACAAGATAAAGGGAAGATTATGGGTATATATGTGAAGGGTGTCCTTGTTACCAACAAATGGTGTCCGAGTCATGTCCCTGATTTAAAAATGTTGGTAGAATCCTCAGATGCTAAACAAAGAAATTGTGAGCTTCGAAGAAGTAGTTAGGGTGAAGATCCGTGGTTGGGCTCTATTTGACAGGCGGTATACTCGAGAGAAGATGAATTGTGTCCTAGATAAGGGCAAACTCTTAGTAAAACGGTAAAACTAGATAGTTGGATGGATAGATGACTATTTGAGGGGAGGATTGTTGGAGTGCGACCAAAGTCCTAAATTGGCTAGATAAAGGGAAGATCATGGGTATATATGTGAGGGAAAATATCTTCATTGGTATGAGGTATTTTAAATAGAACTAAAAGCAAAGTCATGAGGGTTTATGCCCAAAATGGACAATATCATACCATTGTGGAAGATTTGTGGAGTATCCAATGTCATTAATACAGTTGATGCTTTTTGTTTGAAGGCATATTTCTACTTATAGTTAATCGAGTGAGACCTTTAGAAACTAATGCCCCGTTTGATAACCATTTGATTTTTAAAAATTAAGCTTGAAAACACTACATCCACCTATGGGTTTCTATGTTTTATTATATACGTTTTACAAATATTTTCAAAATCTGAGTTTTGAAAACTAAAAAAATGTAGGTTTTGAATTTTTTTTTTTTTTAAAGAATTTGACCAAGCATTCTTTAAACTTAAAAATTGATTATCTATTATTGATTTTTGGAGCGCAAATGGTTTTACTTTACATGATTAACTGTCGGAAAATTTCAACACTTCTTTTTACTTTCATACCACTGATTGTGGATTTTCCACCTTCATTTTACTGGATTTAAATGAAAAATCGATTACCTATGTGTGGTCTTATTACTACCAATATATGCATTTCTGATAAAACACAAGTCATAATGTACATTCTTAAGAGGCATCATCAATTGCGTTTGCATACATGGATGCTCATTTCCCTTATGTAACCGAGGTCTCACCCCAAACAGAATTACTAATCATCAATTTATATGGGAAAGTTGAAGAAAGTTTTCCTTTATTTTAATTCCAGGGCAAGAATACCGATAAGCCTCAGCTTTCAATTTTATTTATCAACATGAAATAGGGAAGATTCGAGAGCTCCCTTCTCTCCAAAAGTTCTCCTCAGAGAAATACAAGACCCACAATAGCAAAAAGATACCCTCCCCTCAGGACACACCTCTCTTATAACAAACTTTCTCACTTCCCTCACTCTACCCCCAACGTACAGAATACACGCAGGCCCATCTACAATCCGAATTCCCTAATTACCCCTTTACTCCCCGCTCTCCTTCTTCCTTACACGTCTATGCTCATAAACTTTGGTTATTTGGGGTTTATCAAATACATTACCTGACTCTGCCCTTCACCTCTCTTCTTATTTTATTTTTTTTTGTTATGTGATAATTCAAATTTTCATATGTATGCATATACATTATTAGGAATTCTAATCTTGTCTATGTTTGTATCACTACGATACTCGAAGAGTTGGCCATATGTAACTCGCCACAGGAGACCTAGTCCATTCAAATAAATGGGAACAGACTAACAGAGCCTTGTAGGACTTGATTCCCTTAAAGGACAGATCTGATCCATCCTCATTAAGTTTCTTTCCCTTGTTGTATTAAGCATAAAGACTAATTCATTGTCAACATGCACATTCTTTTATTCATATAAGGTGAGCAAACAGAATACAGAAGCCTTCACTATCATCTCACATAATTACGAAGCCTCATTTTAGCGAAACAGGATTTATATTTATGCTTGTGACCTCAGATATTGTTCATACTGCTATGCTAATTTTTTTCTGTTGGGGGTCCTGATTTTAACAGGGAACAGTAGTTCTTGTTTTTCAACCAGCTGAGGAAGGAGGTGGGGGAGCAAAGAAAGTGTTAGAGGCTGGAGTACTGGATAATGTTGATGCCATCTTTGGATTGCATGTAAGTCCCAGTTATCCTTTTGGTATGGTGGTCTCCAGGCCTGGTCCTATTCTGGCTGCAAGTGGTTTCTTTGAGGCAGTTATTAGTGGCAAGGGAGGTCATGCTGCTCTACCCCAGCACACAATTGATCCAATTTTAGCAGCTTCCAACATAATTGTCAGTTTGCAGCATCTTATCTCCCGCGAAGCTGATCCTTTAGATTCACAGGTTCTGTTTCTTTCTGAAGTTTTCTCAGGCTTATCGCTACTGCAACTATTGAGCAGTCATACCCTTCTAATTTTATGTGGTAAATCCTAAGTGCTATGTTTTTCGAAGCATAATTCCTTAATTTGTGGTGCAGTTTGTTATTTATCTCAGTTTGAGATTCGTGGTGTTCAACTTTTATCACATTAATGGGAGATCTAAGTTTGTTTTTGTGATTCATTTTTGGGATTTCATGGGAATATGTAATTCTTCTTTCCATACCTCTCTTTGTTATCATAATTTTTTTTCTCCTGAAAAATGTTGTTAATGGCGAGTTTGATATGACTTTTACAACATTAAAAGGTACATTTAATTTAAACGATTGAAAAGCACTCAAAGTTCTTATAAAGAAGTTCTTTGCTGAAAACCTTTTTCAGGAGTCATCGTAAACTTACTAGAATTCAACTAGAATTTTGGCTTTCCTTTCTGATTTTTAGTCTGGAAAAGAACCAGTATGACTGCTTATGTTTCTATTTTAGTTATGATGTTGTTCAAATACTCTTGTCTATATAGTTATGAGAAGGGAGCTTTTGAGGTACCATTTTACAAACTGGTTTAAATCTTTCAGGTTGTTACAGTAGCAAAATTTCAGGGCGGTGGTGCATTCAATGTCATTCCAGATTCTGTTACAATTGGCGGCACTTTCAGGGCATTTTCAAAAGAAAGCTTTATCCAACTTAAACAGCGAATTGTAGAGGTATATTTCTAGAAATATGCTGCCCAAGTCAAAATCCGCAAGTAAAAATAATTCCATAATACTTTCTTCTTCGGAAAAACGATGTCAGGTTATCACAAAACAAGCCAATGTTCAGAGGTGCAACGCCACTGTCGAGTTCAATGAAGATCAAAAGCCTTTTTTTCCAGTTACACTGAATGACCAAGATCTTTACAAGCATTTTCGTGCAGTCGCCGGGGACATGTTGGGTACGAAGAGCATTCTTGATATGCAACCATTGATGGGAGCAGAGGACTTCGCGTTCTATGGAGAAGTGATCCCAGGATTCTTCTACTTTGTTGGGATGACGAACGAAACTCTAGGGAAGTTCAAACCAGCACATTCACCTAATTATATAGTAAATGAAGATGTTCTTCCTTATGGAGCAACATTGCAGGCATCAATCGCCACAAGGTACCTTCTAGAGTACTCACAAAAATCTACTTCTACTAAAGGATACTCTCATGATGAACTTTAA

mRNA sequence

ATGCCTCTACACAGTTGGCTGATTTTGATTTTCATTTTCCATGTGCTTATGCTATGGCCAATTCCTTCGACTTCTTCTCCGAGCCCAGAAGAGTTGTCTCAAATCCCTTTGAATTTCCTTCAACATGCTAAAAATCCAGAGGTTTTTGATTGGATGGTTGATATCAGAAGGAAAATACACGAGAATCCTGAACTGGGTTTCGAGGAATTTGAGACCAGCAAGCTTATCAGAGCGGAGCTTGACCGTTTAGGCATTTCTTATAGATACCCAGTGGCCATCACCGGCGTGGTTGGTTTCATTGGGACTGGAAACCCTCCTTTCGTTGCTATTCGGGCAGATATGGATGCGCTAGCCCTGCAGGAGGGCGTAGAATGGGCACACAAAAGCAAAGTTCCTGGAAAGATGCATGCGTGTGGACATGATGCTCATGTTGCCATGCTTCTAGGGGCTGCAAAGATTCTTCAAGAGCACCGCCGTCTAATACAGGGAACAGTAGTTCTTGTTTTTCAACCAGCTGAGGAAGGAGGTGGGGGAGCAAAGAAAGTGTTAGAGGCTGGAGTACTGGATAATGTTGATGCCATCTTTGGATTGCATGTAAGTCCCAGTTATCCTTTTGGTATGGTGGTCTCCAGGCCTGGTCCTATTCTGGCTGCAAGTGGTTTCTTTGAGGCAGTTATTAGTGGCAAGGGAGGTCATGCTGCTCTACCCCAGCACACAATTGATCCAATTTTAGCAGCTTCCAACATAATTGTCAGTTTGCAGCATCTTATCTCCCGCGAAGCTGATCCTTTAGATTCACAGGTTGTTACAGTAGCAAAATTTCAGGGCGGTGGTGCATTCAATGTCATTCCAGATTCTGTTACAATTGGCGGCACTTTCAGGGCATTTTCAAAAGAAAGCTTTATCCAACTTAAACAGCGAATTGTAGAGGTTATCACAAAACAAGCCAATGTTCAGAGGTGCAACGCCACTGTCGAGTTCAATGAAGATCAAAAGCCTTTTTTTCCAGTTACACTGAATGACCAAGATCTTTACAAGCATTTTCGTGCAGTCGCCGGGGACATGTTGGGTACGAAGAGCATTCTTGATATGCAACCATTGATGGGAGCAGAGGACTTCGCGTTCTATGGAGAAGTGATCCCAGGATTCTTCTACTTTGTTGGGATGACGAACGAAACTCTAGGGAAGTTCAAACCAGCACATTCACCTAATTATATAGTAAATGAAGATGTTCTTCCTTATGGAGCAACATTGCAGGCATCAATCGCCACAAGGTACCTTCTAGAGTACTCACAAAAATCTACTTCTACTAAAGGATACTCTCATGATGAACTTTAA

Coding sequence (CDS)

ATGCCTCTACACAGTTGGCTGATTTTGATTTTCATTTTCCATGTGCTTATGCTATGGCCAATTCCTTCGACTTCTTCTCCGAGCCCAGAAGAGTTGTCTCAAATCCCTTTGAATTTCCTTCAACATGCTAAAAATCCAGAGGTTTTTGATTGGATGGTTGATATCAGAAGGAAAATACACGAGAATCCTGAACTGGGTTTCGAGGAATTTGAGACCAGCAAGCTTATCAGAGCGGAGCTTGACCGTTTAGGCATTTCTTATAGATACCCAGTGGCCATCACCGGCGTGGTTGGTTTCATTGGGACTGGAAACCCTCCTTTCGTTGCTATTCGGGCAGATATGGATGCGCTAGCCCTGCAGGAGGGCGTAGAATGGGCACACAAAAGCAAAGTTCCTGGAAAGATGCATGCGTGTGGACATGATGCTCATGTTGCCATGCTTCTAGGGGCTGCAAAGATTCTTCAAGAGCACCGCCGTCTAATACAGGGAACAGTAGTTCTTGTTTTTCAACCAGCTGAGGAAGGAGGTGGGGGAGCAAAGAAAGTGTTAGAGGCTGGAGTACTGGATAATGTTGATGCCATCTTTGGATTGCATGTAAGTCCCAGTTATCCTTTTGGTATGGTGGTCTCCAGGCCTGGTCCTATTCTGGCTGCAAGTGGTTTCTTTGAGGCAGTTATTAGTGGCAAGGGAGGTCATGCTGCTCTACCCCAGCACACAATTGATCCAATTTTAGCAGCTTCCAACATAATTGTCAGTTTGCAGCATCTTATCTCCCGCGAAGCTGATCCTTTAGATTCACAGGTTGTTACAGTAGCAAAATTTCAGGGCGGTGGTGCATTCAATGTCATTCCAGATTCTGTTACAATTGGCGGCACTTTCAGGGCATTTTCAAAAGAAAGCTTTATCCAACTTAAACAGCGAATTGTAGAGGTTATCACAAAACAAGCCAATGTTCAGAGGTGCAACGCCACTGTCGAGTTCAATGAAGATCAAAAGCCTTTTTTTCCAGTTACACTGAATGACCAAGATCTTTACAAGCATTTTCGTGCAGTCGCCGGGGACATGTTGGGTACGAAGAGCATTCTTGATATGCAACCATTGATGGGAGCAGAGGACTTCGCGTTCTATGGAGAAGTGATCCCAGGATTCTTCTACTTTGTTGGGATGACGAACGAAACTCTAGGGAAGTTCAAACCAGCACATTCACCTAATTATATAGTAAATGAAGATGTTCTTCCTTATGGAGCAACATTGCAGGCATCAATCGCCACAAGGTACCTTCTAGAGTACTCACAAAAATCTACTTCTACTAAAGGATACTCTCATGATGAACTTTAA

Protein sequence

MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIHENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTIDPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKSILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQASIATRYLLEYSQKSTSTKGYSHDEL
Homology
BLAST of Lag0034511 vs. NCBI nr
Match: XP_038878754.1 (IAA-amino acid hydrolase ILR1-like 4 [Benincasa hispida])

HSP 1 Score: 805.4 bits (2079), Expect = 2.4e-229
Identity = 396/445 (88.99%), Postives = 419/445 (94.16%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           MPLHSWL LI IFH+L+   IPS+SSP+P+ELSQIP  FLQ+AK+PEVFDWMVD+RRKIH
Sbjct: 1   MPLHSWLNLILIFHLLIPLLIPSSSSPNPDELSQIPSIFLQYAKHPEVFDWMVDVRRKIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELGFEEFETSKLIRAELDRLGISY YPVAITGVVG IG GNPPFVAIRADMDALALQ
Sbjct: 61  ENPELGFEEFETSKLIRAELDRLGISYEYPVAITGVVGLIGNGNPPFVAIRADMDALALQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           EGVEW HKSKVPGKMHACGHDAHVAMLLGAAKILQ+HR LIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQHRHLIQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K+LEAGVLDNVDAIFGLHVS  +PFGMV+S+PGPILAASGFFEAVI GKGGHAALPQHTI
Sbjct: 181 KMLEAGVLDNVDAIFGLHVSSRHPFGMVISKPGPILAASGFFEAVIGGKGGHAALPQHTI 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASNIIVSLQHL+SREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFSKES
Sbjct: 241 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVT+NDQ+LYKHF+ +AGDMLGTK+
Sbjct: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTMNDQNLYKHFQTIAGDMLGTKN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
           +LDMQPLMG EDF FYGEVIPG FYFVGM NET+GKF   HSP YIVNEDVLPYGA LQA
Sbjct: 361 VLDMQPLMGTEDFGFYGEVIPGLFYFVGMRNETVGKFDQGHSPYYIVNEDVLPYGAALQA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           SIATRYLLEYS KSTSTKG+SHDEL
Sbjct: 421 SIATRYLLEYSPKSTSTKGHSHDEL 445

BLAST of Lag0034511 vs. NCBI nr
Match: KAG6588014.1 (IAA-amino acid hydrolase ILR1-like 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 802.4 bits (2071), Expect = 2.0e-228
Identity = 399/445 (89.66%), Postives = 414/445 (93.03%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           M L   L LIF+FHVLML PIP  SSP PEELSQIPLNFLQ+AK+PEVFDWMVDIRRKIH
Sbjct: 1   MALRCCLNLIFVFHVLMLCPIPLCSSPIPEELSQIPLNFLQYAKSPEVFDWMVDIRRKIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELGFEEFETSKLIRAELDRLGISY YPVAITGVVGFIGTGNPPFVAIRADMDALALQ
Sbjct: 61  ENPELGFEEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           EGV W H+SKVPGKMHACGHDAHVAMLLGAAKIL EHR LIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVVWEHQSKVPGKMHACGHDAHVAMLLGAAKILVEHRHLIQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K+LE GVL+NVDAIFG HVSP YPFGMV+SRPGPILAASGFFEAVISGKGGHAALP HT 
Sbjct: 181 KMLETGVLNNVDAIFGAHVSPKYPFGMVISRPGPILAASGFFEAVISGKGGHAALPHHTF 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASNII+SLQ LISRE DPLDSQVVTVAKFQGG AFNVIPDSVTIGGTFRAFSKES
Sbjct: 241 DPILAASNIIISLQQLISRETDPLDSQVVTVAKFQGGDAFNVIPDSVTIGGTFRAFSKES 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           FIQLK RIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLY+HFRAVAGDMLG+K+
Sbjct: 301 FIQLKHRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYEHFRAVAGDMLGSKN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
           +LDMQPLMGAEDFAFY E  PGFFYFVGM NET+GKF+P HSP YIVNEDV+PYGA LQA
Sbjct: 361 VLDMQPLMGAEDFAFYCEAFPGFFYFVGMKNETVGKFEPGHSPYYIVNEDVIPYGAALQA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           SIATRYLLEYS KSTSTKGYSHDEL
Sbjct: 421 SIATRYLLEYSPKSTSTKGYSHDEL 445

BLAST of Lag0034511 vs. NCBI nr
Match: KAG7021909.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 800.8 bits (2067), Expect = 5.9e-228
Identity = 398/445 (89.44%), Postives = 413/445 (92.81%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           M L   L LIF+FHVLML PIP  SSP PEELSQIPLNFLQ+AK+PEVFDWMVDIRRKIH
Sbjct: 1   MALRCCLNLIFVFHVLMLCPIPLCSSPIPEELSQIPLNFLQYAKSPEVFDWMVDIRRKIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELGFEEFETSKLIRAELDRLGISY YPVAITGVVGFIGTGNPPFVAIRADMDALALQ
Sbjct: 61  ENPELGFEEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           EGV W H+SKVPGKMHACGHD HVAMLLGAAKIL EHR LIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVVWEHQSKVPGKMHACGHDGHVAMLLGAAKILVEHRHLIQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K+LE GVL+NVDAIFG HVSP YPFGMV+SRPGPILAASGFFEAVISGKGGHAALP HT 
Sbjct: 181 KMLETGVLNNVDAIFGAHVSPKYPFGMVISRPGPILAASGFFEAVISGKGGHAALPHHTF 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASNII+SLQ LISRE DPLDSQVVTVAKFQGG AFNVIPDSVTIGGTFRAFSKES
Sbjct: 241 DPILAASNIIISLQQLISRETDPLDSQVVTVAKFQGGDAFNVIPDSVTIGGTFRAFSKES 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           FIQLK RIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLY+HFRAVAGDMLG+K+
Sbjct: 301 FIQLKHRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYEHFRAVAGDMLGSKN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
           +LDMQPLMGAEDFAFY E  PGFFYFVGM NET+GKF+P HSP YIVNEDV+PYGA LQA
Sbjct: 361 VLDMQPLMGAEDFAFYCEAFPGFFYFVGMKNETVGKFEPGHSPYYIVNEDVIPYGAALQA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           SIATRYLLEYS KSTSTKGYSHDEL
Sbjct: 421 SIATRYLLEYSPKSTSTKGYSHDEL 445

BLAST of Lag0034511 vs. NCBI nr
Match: XP_023530622.1 (IAA-amino acid hydrolase ILR1-like 4 [Cucurbita pepo subsp. pepo] >XP_023530623.1 IAA-amino acid hydrolase ILR1-like 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 795.0 bits (2052), Expect = 3.2e-226
Identity = 397/445 (89.21%), Postives = 412/445 (92.58%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           M L   L LIF+FHVLML PIP  SSP PEELSQIPLNFLQ+AK+PEVFDWMVDIRRKIH
Sbjct: 1   MALGCCLNLIFVFHVLMLCPIPLCSSPIPEELSQIPLNFLQYAKSPEVFDWMVDIRRKIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELGFEEFETSKLIRAELDRLGISY YPVAITGVVGFIGTGNPPFVAIRADMDALALQ
Sbjct: 61  ENPELGFEEFETSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           EGV W H+SKVPGKMHACGHDAHVAMLLGAAKIL EHR LIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVVWEHRSKVPGKMHACGHDAHVAMLLGAAKILVEHRHLIQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K+LE GVL+NVDAIFG HVS  YPFGMV+SRPGPILAASGFFEAVISGKGGHAALP HT 
Sbjct: 181 KMLETGVLNNVDAIFGAHVSAKYPFGMVISRPGPILAASGFFEAVISGKGGHAALPHHTF 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASNII+SLQ LISRE DPLDSQVVTVAKFQGG AFNVIPDSVTIGGTFRAFSKES
Sbjct: 241 DPILAASNIIISLQQLISRETDPLDSQVVTVAKFQGGDAFNVIPDSVTIGGTFRAFSKES 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           FIQLK RIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLY+HFRAVAGD+LG+K+
Sbjct: 301 FIQLKHRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYEHFRAVAGDLLGSKN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
           ILDMQPLMGAEDFAFY E  PGFFYFVGM NET+GKF+P HSP YIVNEDV+PYGA LQA
Sbjct: 361 ILDMQPLMGAEDFAFYCEAFPGFFYFVGMKNETVGKFEPGHSPYYIVNEDVIPYGAALQA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           SIATRYLLEYS KSTSTKGYS DEL
Sbjct: 421 SIATRYLLEYSPKSTSTKGYSRDEL 445

BLAST of Lag0034511 vs. NCBI nr
Match: XP_022933830.1 (IAA-amino acid hydrolase ILR1-like 4 isoform X1 [Cucurbita moschata])

HSP 1 Score: 793.9 bits (2049), Expect = 7.2e-226
Identity = 395/445 (88.76%), Postives = 412/445 (92.58%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           M L   L LIF+FHVLML PIP  SSP PEELSQIPLNFLQ+AK+PEVFDWMVDIRRKIH
Sbjct: 1   MALRCCLNLIFVFHVLMLCPIPLCSSPIPEELSQIPLNFLQYAKSPEVFDWMVDIRRKIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELGFEEF+TSKLIRAELDRLGISY YPVAITGVVGFIGTGNPPFVAIRADMDALALQ
Sbjct: 61  ENPELGFEEFQTSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           EGV W H+SKVPGKMHACGHDAHVAMLLGAAKIL EHR LIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVVWEHQSKVPGKMHACGHDAHVAMLLGAAKILVEHRHLIQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K+LE GVL+NVDAIFG HVSP YPFGMV+SRPGPILAASGFFEAVISGKGGHAALP HT 
Sbjct: 181 KMLETGVLNNVDAIFGAHVSPKYPFGMVISRPGPILAASGFFEAVISGKGGHAALPHHTF 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASNII+SLQ LISRE DPLDSQVVTVAKFQGG AFNVIPDSVTIGGTFRAFS+ES
Sbjct: 241 DPILAASNIIISLQQLISRETDPLDSQVVTVAKFQGGDAFNVIPDSVTIGGTFRAFSRES 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           FIQLK RIVEVITKQANVQRCNATVEFNEDQKP FPVTLNDQDLY+HFRAVAGDMLG+K+
Sbjct: 301 FIQLKHRIVEVITKQANVQRCNATVEFNEDQKPSFPVTLNDQDLYEHFRAVAGDMLGSKN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
           +LDMQPLMGAEDFAFY E  PGFFYFVGM NET+GKF+P HSP YIVNEDV+PYGA LQA
Sbjct: 361 VLDMQPLMGAEDFAFYCEAFPGFFYFVGMKNETVGKFEPGHSPYYIVNEDVIPYGAALQA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           SIATRYLLEYS KSTSTKGYS DEL
Sbjct: 421 SIATRYLLEYSPKSTSTKGYSRDEL 445

BLAST of Lag0034511 vs. ExPASy Swiss-Prot
Match: O04373 (IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=1 SV=2)

HSP 1 Score: 610.1 bits (1572), Expect = 1.9e-173
Identity = 302/445 (67.87%), Postives = 362/445 (81.35%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           M    W+  + I H+L     P+  S S   LSQIP  FL  AK  + FDWMV IRR+IH
Sbjct: 1   MSFFKWVSFVLILHLLN----PTLISCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELG+EE ETSKL+RAEL+++G+SY+YPVA+TGVVG++GTG+ PFVA+RADMDALA+Q
Sbjct: 61  ENPELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           E VEW HKSKVPGKMHACGHDAH  MLLGAAK+L+EH   +QGTVVLVFQPAEEGGGGAK
Sbjct: 121 EMVEWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K++EAGVL+NV AIFGLHV+     G V SR GP+LA SGFF+A ISGKGGHAALPQHTI
Sbjct: 181 KIVEAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTI 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASN+IVSLQHL+SREADPLDSQVVTVAKF+GGGAFNVIPDSVTIGGTFRAFS +S
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKS 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           F+QLK+RI +VIT+QA+V  CNATV+F E++KPFFP T+ND+ L++ F+ V+GDMLG ++
Sbjct: 301 FMQLKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIEN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
            ++MQPLMG+EDF+FY + IPG F FVGM N+        HSP + VNE++LPYGA+L A
Sbjct: 361 YVEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           S+ATRYLLE  + ST  K    DEL
Sbjct: 421 SMATRYLLEL-KASTLNKSNKKDEL 440

BLAST of Lag0034511 vs. ExPASy Swiss-Prot
Match: Q84XG9 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.3e-166
Identity = 286/411 (69.59%), Postives = 335/411 (81.51%), Query Frame = 0

Query: 36  PLNFLQHAKNPEVFDWMVDIRRKIHENPELGFEEFETSKLIRAELDRLGISYRYPVAITG 95
           P   L+ AK  E   WMV +RR+IHENPELG+EEF TS+L+R ELD LGI YR+P A+TG
Sbjct: 33  PAGLLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTG 92

Query: 96  VVGFIGTGNPPFVAIRADMDALALQEGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQ 155
           VV  +GTG PPFVA+RADMDAL +QE VEW HKSKVPGKMH CGHDAHVAMLLG+A+ILQ
Sbjct: 93  VVATVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQ 152

Query: 156 EHRRLIQGTVVLVFQPAEEGGGGAKKVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPI 215
           EHR  ++GTVVLVFQPAEEGGGGAKK+++ G ++N++AIFG+HV+   P G+V SRPGP+
Sbjct: 153 EHRDELKGTVVLVFQPAEEGGGGAKKMIDDGTVENIEAIFGVHVADVVPIGVVASRPGPV 212

Query: 216 LAASGFFEAVISGKGGHAALPQHTIDPILAASNIIVSLQHLISREADPLDSQVVTVAKFQ 275
           +A SGFFEAVISGKGGHAALP HTIDPILAASN+IVSLQ L+SREADPLDSQVVTV KFQ
Sbjct: 213 MAGSGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQ 272

Query: 276 GGGAFNVIPDSVTIGGTFRAFSKESFIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFF 335
           GGGAFNVIPDSVTIGGTFRAF KESF QLKQRI EVI  QA+VQRCNA V+F +  +PFF
Sbjct: 273 GGGAFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFF 332

Query: 336 PVTLNDQDLYKHFRAVAGDMLGTKSILDMQPLMGAEDFAFYGEVIPG-FFYFVGMTNETL 395
           P T+N   L+  F  VA +M+G K++ D QPLMGAEDFAFY + IP  ++YF+GM NET 
Sbjct: 333 PPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETR 392

Query: 396 GKFKPAHSPNYIVNEDVLPYGATLQASIATRYLLEYSQKSTSTKGYSHDEL 446
           G   P HSP + +NED LPYGA LQAS+ATRYLLE+ Q  T+ K  +HDEL
Sbjct: 393 GPQAPHHSPYFTINEDALPYGAALQASLATRYLLEH-QPPTTGKAKAHDEL 442

BLAST of Lag0034511 vs. ExPASy Swiss-Prot
Match: Q8S9S4 (IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=ILL1 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 5.1e-166
Identity = 285/411 (69.34%), Postives = 334/411 (81.27%), Query Frame = 0

Query: 36  PLNFLQHAKNPEVFDWMVDIRRKIHENPELGFEEFETSKLIRAELDRLGISYRYPVAITG 95
           P   L+ AK  E   WMV +RR+IHENPELG+EEF TS+L+R ELD LGI YR+P A+TG
Sbjct: 33  PAGLLRRAKEAEFAGWMVGLRRRIHENPELGYEEFATSELVRRELDALGIPYRHPFAVTG 92

Query: 96  VVGFIGTGNPPFVAIRADMDALALQEGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQ 155
           VV  +GTG PPFVA+RADMDAL +QE VEW HKSKVPGKMH CGHDAHVAMLLG+A+ILQ
Sbjct: 93  VVATVGTGGPPFVALRADMDALPMQESVEWEHKSKVPGKMHGCGHDAHVAMLLGSARILQ 152

Query: 156 EHRRLIQGTVVLVFQPAEEGGGGAKKVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPI 215
           EHR  ++GTVVLVFQPAEEGGGGAKK+++ G ++N++AIFG+HV+   P G+V SRPGP+
Sbjct: 153 EHRDELKGTVVLVFQPAEEGGGGAKKMIDDGAVENIEAIFGVHVADVVPIGVVASRPGPV 212

Query: 216 LAASGFFEAVISGKGGHAALPQHTIDPILAASNIIVSLQHLISREADPLDSQVVTVAKFQ 275
           +A SGFFEAVISGKGGHAALP HTIDPILAASN+IVSLQ L+SREADPLDSQVVTV KFQ
Sbjct: 213 MAGSGFFEAVISGKGGHAALPHHTIDPILAASNVIVSLQQLVSREADPLDSQVVTVGKFQ 272

Query: 276 GGGAFNVIPDSVTIGGTFRAFSKESFIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFF 335
           GGGAFNVIPDSVTIGGTFRAF KESF QLKQRI EVI  QA+VQRCNA V+F +  +PFF
Sbjct: 273 GGGAFNVIPDSVTIGGTFRAFLKESFNQLKQRIEEVIVSQASVQRCNAVVDFLDKDRPFF 332

Query: 336 PVTLNDQDLYKHFRAVAGDMLGTKSILDMQPLMGAEDFAFYGEVIPG-FFYFVGMTNETL 395
           P T+N   L+  F  VA +M+G K++ D QPLMGAEDFAFY + IP  ++YF+GM NET 
Sbjct: 333 PPTINSAGLHDFFVKVASEMVGPKNVRDKQPLMGAEDFAFYADAIPATYYYFLGMYNETR 392

Query: 396 GKFKPAHSPNYIVNEDVLPYGATLQASIATRYLLEYSQKSTSTKGYSHDEL 446
           G   P HSP + +NED LPYGA LQAS+A RYLLE+ Q  T+ K  +HDEL
Sbjct: 393 GPQAPHHSPYFTINEDALPYGAALQASLAARYLLEH-QPPTTGKAKAHDEL 442

BLAST of Lag0034511 vs. ExPASy Swiss-Prot
Match: Q9SWX9 (IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana OX=3702 GN=ILL5 PE=3 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 1.2e-154
Identity = 267/406 (65.76%), Postives = 329/406 (81.03%), Query Frame = 0

Query: 26  SPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIHENPELGFEEFETSKLIRAELDRLGI 85
           S S  +LSQIP NFL  AK  + FDWMV IRR+IHENPELG+EE ETSKL++ ELD++G+
Sbjct: 22  SCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGV 81

Query: 86  SYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWAHKSKVPGKMHACGHDAHVA 145
           SY+ PVA+TGV+G++GTG+ PFVA+RADMDAL +QE VEW HKSK+PGKMHACGHDAH  
Sbjct: 82  SYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHTT 141

Query: 146 MLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKVLEAGVLDNVDAIFGLHVSPSYPF 205
           MLLGAAK+L+EH+  +QGTV+LVFQPAEEGG GAKK++EAGVL+NV AIFGLHVS     
Sbjct: 142 MLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLGL 201

Query: 206 GMVVSRPGPILAASGFFEAVISGKGGHAALPQHTIDPILAASNIIVSLQHLISREADPLD 265
           G + SR G ++A SG F+A ISGKGGHAALPQ  IDP+LAASN+I+SLQHL+SREADPLD
Sbjct: 202 GQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPLD 261

Query: 266 SQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQRIVEVITKQANVQRCNATV 325
           SQVVTVA F+G  AFNVIPDSVTIGGTFRA   +SF QLKQRIV+VIT QA+V  CNATV
Sbjct: 262 SQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNATV 321

Query: 326 EFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKSILDMQPLMGAEDFAFYGEVIPGFFY 385
           +F ED+ P FP T+N++ L+  ++ V+ DMLG ++ ++  P+M +EDFAFY + IPG F 
Sbjct: 322 DFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHFS 381

Query: 386 FVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQASIATRYLLEYS 432
           FVGM N++       HSP + VNE++LPYGA+L AS+ATRYLL+ S
Sbjct: 382 FVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYLLDSS 427

BLAST of Lag0034511 vs. ExPASy Swiss-Prot
Match: P54970 (IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=1 SV=2)

HSP 1 Score: 543.9 bits (1400), Expect = 1.7e-153
Identity = 277/448 (61.83%), Postives = 341/448 (76.12%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSP---EELSQIPLNFLQHAKNPEVFDWMVDIRR 60
           M L+  L L F   + +L    S SS SP   E+ SQI    L+ AK+PEVFDWMV IRR
Sbjct: 1   MALNKLLSLTFQLLLFLL----SVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRR 60

Query: 61  KIHENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDAL 120
           KIHENPELG+EE ETSKLIR+EL+ +GI YRYPVAITGV+G+IGTG PPFVA+RADMDAL
Sbjct: 61  KIHENPELGYEELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDAL 120

Query: 121 ALQEGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGG 180
            +QEGVEW HKSK+ GKMHACGHD HV MLLGAAKIL EHR  +QGTVVL+FQPAEEG  
Sbjct: 121 PIQEGVEWEHKSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLS 180

Query: 181 GAKKVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQ 240
           GAKK+ E G L NV+AIFG+H+S   PFG   SR G  LA +G FEAVI+GKGGHAA+PQ
Sbjct: 181 GAKKMREEGALKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQ 240

Query: 241 HTIDPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFS 300
           HTIDP++AAS+I++SLQ L+SRE DPLDS+VVTV+K  GG AFNVIPDS+TIGGT RAF+
Sbjct: 241 HTIDPVVAASSIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT 300

Query: 301 KESFIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLG 360
              F QL+QR+ EVITKQA V RCNA+V    + +   P T+N++DLYK F+ V  D+LG
Sbjct: 301 --GFTQLQQRVKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLG 360

Query: 361 TKSILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGAT 420
            ++ ++  P+MG+EDF+++ E IPG F  +GM +ET G +  +HSP Y +NEDVLPYGA 
Sbjct: 361 QEAFVEAAPVMGSEDFSYFAETIPGHFSLLGMQDETNG-YASSHSPLYRINEDVLPYGAA 420

Query: 421 LQASIATRYLLEYSQKSTSTKGYSHDEL 446
           + AS+A +YL E + K  S  G+ H+EL
Sbjct: 421 IHASMAVQYLKEKASKG-SVSGF-HEEL 439

BLAST of Lag0034511 vs. ExPASy TrEMBL
Match: A0A6J1F5X7 (IAA-amino acid hydrolase ILR1-like 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441127 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.5e-226
Identity = 395/445 (88.76%), Postives = 412/445 (92.58%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           M L   L LIF+FHVLML PIP  SSP PEELSQIPLNFLQ+AK+PEVFDWMVDIRRKIH
Sbjct: 1   MALRCCLNLIFVFHVLMLCPIPLCSSPIPEELSQIPLNFLQYAKSPEVFDWMVDIRRKIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELGFEEF+TSKLIRAELDRLGISY YPVAITGVVGFIGTGNPPFVAIRADMDALALQ
Sbjct: 61  ENPELGFEEFQTSKLIRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           EGV W H+SKVPGKMHACGHDAHVAMLLGAAKIL EHR LIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVVWEHQSKVPGKMHACGHDAHVAMLLGAAKILVEHRHLIQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K+LE GVL+NVDAIFG HVSP YPFGMV+SRPGPILAASGFFEAVISGKGGHAALP HT 
Sbjct: 181 KMLETGVLNNVDAIFGAHVSPKYPFGMVISRPGPILAASGFFEAVISGKGGHAALPHHTF 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASNII+SLQ LISRE DPLDSQVVTVAKFQGG AFNVIPDSVTIGGTFRAFS+ES
Sbjct: 241 DPILAASNIIISLQQLISRETDPLDSQVVTVAKFQGGDAFNVIPDSVTIGGTFRAFSRES 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           FIQLK RIVEVITKQANVQRCNATVEFNEDQKP FPVTLNDQDLY+HFRAVAGDMLG+K+
Sbjct: 301 FIQLKHRIVEVITKQANVQRCNATVEFNEDQKPSFPVTLNDQDLYEHFRAVAGDMLGSKN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
           +LDMQPLMGAEDFAFY E  PGFFYFVGM NET+GKF+P HSP YIVNEDV+PYGA LQA
Sbjct: 361 VLDMQPLMGAEDFAFYCEAFPGFFYFVGMKNETVGKFEPGHSPYYIVNEDVIPYGAALQA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           SIATRYLLEYS KSTSTKGYS DEL
Sbjct: 421 SIATRYLLEYSPKSTSTKGYSRDEL 445

BLAST of Lag0034511 vs. ExPASy TrEMBL
Match: A0A5D3CIQ7 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002720 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.3e-224
Identity = 391/445 (87.87%), Postives = 415/445 (93.26%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           MPL S L LI IFH+L+   IPS+SS  PEELSQIP  FLQ+AK+PEVFDWMVDIRRKIH
Sbjct: 1   MPLCSSLSLILIFHLLISLLIPSSSSSIPEELSQIPSFFLQYAKHPEVFDWMVDIRRKIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELGF+EFETSKLIRAELDRLGISY YPVA+TGVVGFIGTGNPPFVAIRADMDALALQ
Sbjct: 61  ENPELGFQEFETSKLIRAELDRLGISYEYPVAVTGVVGFIGTGNPPFVAIRADMDALALQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           EGVEW HKSKVPGKMHACGHDAHVAMLLGAAKILQ++ RLIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K+LEAGVLDNVDAIFGLH+S  YPFG+ +S+PGPILAASGFFE+VI GKGGHAALPQHTI
Sbjct: 181 KILEAGVLDNVDAIFGLHLSSRYPFGLAISKPGPILAASGFFESVIGGKGGHAALPQHTI 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASNIIVSLQHL+SREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFSKES
Sbjct: 241 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           FIQLKQRIVEVITKQA VQRCNATVEFNEDQKPFFPVTLNDQ+LYKHFR VA DMLGTK+
Sbjct: 301 FIQLKQRIVEVITKQAKVQRCNATVEFNEDQKPFFPVTLNDQNLYKHFRTVAVDMLGTKN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
           ILDMQPLMGAEDF FYGEVIPG FYF+GM NET+GKF+  HSP YIVNEDVLPYGA LQA
Sbjct: 361 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           SIA RYL+EYS KSTSTKG+SHDEL
Sbjct: 421 SIAARYLIEYSPKSTSTKGHSHDEL 445

BLAST of Lag0034511 vs. ExPASy TrEMBL
Match: A0A1S3BQ36 (IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo OX=3656 GN=LOC103492283 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.3e-224
Identity = 391/445 (87.87%), Postives = 415/445 (93.26%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           MPL S L LI IFH+L+   IPS+SS  PEELSQIP  FLQ+AK+PEVFDWMVDIRRKIH
Sbjct: 1   MPLCSSLSLILIFHLLISLLIPSSSSSIPEELSQIPSFFLQYAKHPEVFDWMVDIRRKIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELGF+EFETSKLIRAELDRLGISY YPVA+TGVVGFIGTGNPPFVAIRADMDALALQ
Sbjct: 61  ENPELGFQEFETSKLIRAELDRLGISYEYPVAVTGVVGFIGTGNPPFVAIRADMDALALQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           EGVEW HKSKVPGKMHACGHDAHVAMLLGAAKILQ++ RLIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K+LEAGVLDNVDAIFGLH+S  YPFG+ +S+PGPILAASGFFE+VI GKGGHAALPQHTI
Sbjct: 181 KILEAGVLDNVDAIFGLHLSSRYPFGLAISKPGPILAASGFFESVIGGKGGHAALPQHTI 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASNIIVSLQHL+SREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFSKES
Sbjct: 241 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           FIQLKQRIVEVITKQA VQRCNATVEFNEDQKPFFPVTLNDQ+LYKHFR VA DMLGTK+
Sbjct: 301 FIQLKQRIVEVITKQAKVQRCNATVEFNEDQKPFFPVTLNDQNLYKHFRTVAVDMLGTKN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
           ILDMQPLMGAEDF FYGEVIPG FYF+GM NET+GKF+  HSP YIVNEDVLPYGA LQA
Sbjct: 361 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETIGKFEQGHSPYYIVNEDVLPYGAALQA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           SIA RYL+EYS KSTSTKG+SHDEL
Sbjct: 421 SIAARYLIEYSPKSTSTKGHSHDEL 445

BLAST of Lag0034511 vs. ExPASy TrEMBL
Match: A0A0A0LYS1 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G575150 PE=3 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 5.8e-221
Identity = 386/445 (86.74%), Postives = 412/445 (92.58%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           MP  S+L LI IFH+L+   IPS+SSP P+ELSQIP  FLQ+A++PEVFDWMV IRRKIH
Sbjct: 1   MPQRSFLNLILIFHILISLLIPSSSSPIPDELSQIPSFFLQYAQHPEVFDWMVGIRRKIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELGF+EFETSKLIRAELDRLG+SY YPVAITGVVGFIGTGNPPFVAIRADMDAL LQ
Sbjct: 61  ENPELGFQEFETSKLIRAELDRLGVSYEYPVAITGVVGFIGTGNPPFVAIRADMDALPLQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           EGVEW HKSKVPGKMHACGHDAHVAMLLGAAKILQ++ RLIQGTVVLVFQPAEEGGGGAK
Sbjct: 121 EGVEWEHKSKVPGKMHACGHDAHVAMLLGAAKILQQNSRLIQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K+LEAGVLD VDAIFGLHVS SYPFGM +S+PGPILAASGFFEAVI GKGGHAALPQHTI
Sbjct: 181 KMLEAGVLDKVDAIFGLHVSSSYPFGMAISKPGPILAASGFFEAVIGGKGGHAALPQHTI 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASNIIVSLQHL+SREADPLDSQVVT+AKFQGGGAFNVIPDSVTIGGTFRAFSKES
Sbjct: 241 DPILAASNIIVSLQHLVSREADPLDSQVVTIAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           FIQLKQRIVEVIT QA VQRCNATVEFNEDQKPFFPVT+N+ +LYKHFR VA DMLGTK+
Sbjct: 301 FIQLKQRIVEVITNQAKVQRCNATVEFNEDQKPFFPVTVNNHNLYKHFRTVAVDMLGTKN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
           ILDMQPLMGAEDF FYGEVIPG FYF+GM NET+GKF+  HSP YIVNEDVLPYGA LQA
Sbjct: 361 ILDMQPLMGAEDFGFYGEVIPGLFYFLGMKNETVGKFEQGHSPYYIVNEDVLPYGAALQA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           SIATRYLLE+S KSTSTK +SHDEL
Sbjct: 421 SIATRYLLEHSPKSTSTKEHSHDEL 445

BLAST of Lag0034511 vs. ExPASy TrEMBL
Match: A0A6J1KT14 (IAA-amino acid hydrolase ILR1-like 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498399 PE=3 SV=1)

HSP 1 Score: 772.3 bits (1993), Expect = 1.1e-219
Identity = 382/436 (87.61%), Postives = 402/436 (92.20%), Query Frame = 0

Query: 10  IFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIHENPELGFEE 69
           + +F + + +     SSP PEELSQIPLNFLQ+AK+PEVFDWMVDIRRKIHENPELGFEE
Sbjct: 9   LILFSMCLCYAQFLCSSPIPEELSQIPLNFLQYAKSPEVFDWMVDIRRKIHENPELGFEE 68

Query: 70  FETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWAHKS 129
           FETSKL+RAELDRLGISY YPVAITGVVGFIGTGNPPFVAIRADMDALALQEGV W HKS
Sbjct: 69  FETSKLVRAELDRLGISYEYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVAWEHKS 128

Query: 130 KVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKVLEAGVLD 189
           KVPGKMHACGHDAHVAMLLGAAKIL EHR LIQGT+VLVFQPAEEGGGGAKK+LE GVL+
Sbjct: 129 KVPGKMHACGHDAHVAMLLGAAKILVEHRHLIQGTLVLVFQPAEEGGGGAKKMLETGVLN 188

Query: 190 NVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTIDPILAASNI 249
           NVDAIFG HVS  YPFGMV+SRPGPILAASGFFEAVISGKGGHAALP HT DPILAASNI
Sbjct: 189 NVDAIFGAHVSAKYPFGMVISRPGPILAASGFFEAVISGKGGHAALPHHTFDPILAASNI 248

Query: 250 IVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQRIV 309
           I+SLQ LISRE DPLDSQVVTVAKFQGG AFNVIPDSVTIGGTFRAFSKESFIQLK RIV
Sbjct: 249 IISLQQLISRETDPLDSQVVTVAKFQGGDAFNVIPDSVTIGGTFRAFSKESFIQLKHRIV 308

Query: 310 EVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKSILDMQPLMG 369
           EVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLY+HFRAVAGDMLG+K++LDMQPLMG
Sbjct: 309 EVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYEHFRAVAGDMLGSKNVLDMQPLMG 368

Query: 370 AEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQASIATRYLLE 429
           AEDFAFY E  PGFFYFVGM NET+GKF+P HSP YIVNEDV+PYGA LQASIATRYLLE
Sbjct: 369 AEDFAFYCEAFPGFFYFVGMKNETVGKFEPGHSPYYIVNEDVIPYGAALQASIATRYLLE 428

Query: 430 YSQKSTSTKGYSHDEL 446
           YS KSTSTKGYS DEL
Sbjct: 429 YSPKSTSTKGYSRDEL 444

BLAST of Lag0034511 vs. TAIR 10
Match: AT1G51760.1 (peptidase M20/M25/M40 family protein )

HSP 1 Score: 610.1 bits (1572), Expect = 1.4e-174
Identity = 302/445 (67.87%), Postives = 362/445 (81.35%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIH 60
           M    W+  + I H+L     P+  S S   LSQIP  FL  AK  + FDWMV IRR+IH
Sbjct: 1   MSFFKWVSFVLILHLLN----PTLISCSSNGLSQIPSKFLTLAKRNDFFDWMVGIRRRIH 60

Query: 61  ENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQ 120
           ENPELG+EE ETSKL+RAEL+++G+SY+YPVA+TGVVG++GTG+ PFVA+RADMDALA+Q
Sbjct: 61  ENPELGYEEVETSKLVRAELEKMGVSYKYPVAVTGVVGYVGTGHAPFVALRADMDALAMQ 120

Query: 121 EGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAK 180
           E VEW HKSKVPGKMHACGHDAH  MLLGAAK+L+EH   +QGTVVLVFQPAEEGGGGAK
Sbjct: 121 EMVEWEHKSKVPGKMHACGHDAHTTMLLGAAKLLKEHEEELQGTVVLVFQPAEEGGGGAK 180

Query: 181 KVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTI 240
           K++EAGVL+NV AIFGLHV+     G V SR GP+LA SGFF+A ISGKGGHAALPQHTI
Sbjct: 181 KIVEAGVLENVSAIFGLHVTNQLALGQVSSREGPMLAGSGFFKAKISGKGGHAALPQHTI 240

Query: 241 DPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKES 300
           DPILAASN+IVSLQHL+SREADPLDSQVVTVAKF+GGGAFNVIPDSVTIGGTFRAFS +S
Sbjct: 241 DPILAASNVIVSLQHLVSREADPLDSQVVTVAKFEGGGAFNVIPDSVTIGGTFRAFSTKS 300

Query: 301 FIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKS 360
           F+QLK+RI +VIT+QA+V  CNATV+F E++KPFFP T+ND+ L++ F+ V+GDMLG ++
Sbjct: 301 FMQLKKRIEQVITRQASVNMCNATVDFIEEEKPFFPPTVNDKALHQFFKNVSGDMLGIEN 360

Query: 361 ILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQA 420
            ++MQPLMG+EDF+FY + IPG F FVGM N+        HSP + VNE++LPYGA+L A
Sbjct: 361 YVEMQPLMGSEDFSFYQQAIPGHFSFVGMQNKARSPMASPHSPYFEVNEELLPYGASLHA 420

Query: 421 SIATRYLLEYSQKSTSTKGYSHDEL 446
           S+ATRYLLE  + ST  K    DEL
Sbjct: 421 SMATRYLLEL-KASTLNKSNKKDEL 440

BLAST of Lag0034511 vs. TAIR 10
Match: AT1G51780.1 (IAA-leucine resistant (ILR)-like gene 5 )

HSP 1 Score: 547.7 bits (1410), Expect = 8.4e-156
Identity = 267/406 (65.76%), Postives = 329/406 (81.03%), Query Frame = 0

Query: 26  SPSPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIHENPELGFEEFETSKLIRAELDRLGI 85
           S S  +LSQIP NFL  AK  + FDWMV IRR+IHENPELG+EE ETSKL++ ELD++G+
Sbjct: 22  SCSSNDLSQIPKNFLSLAKREDFFDWMVGIRRRIHENPELGYEEVETSKLVKTELDKMGV 81

Query: 86  SYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWAHKSKVPGKMHACGHDAHVA 145
           SY+ PVA+TGV+G++GTG+ PFVA+RADMDAL +QE VEW HKSK+PGKMHACGHDAH  
Sbjct: 82  SYKNPVAVTGVIGYVGTGHAPFVALRADMDALPIQEMVEWEHKSKIPGKMHACGHDAHTT 141

Query: 146 MLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKVLEAGVLDNVDAIFGLHVSPSYPF 205
           MLLGAAK+L+EH+  +QGTV+LVFQPAEEGG GAKK++EAGVL+NV AIFGLHVS     
Sbjct: 142 MLLGAAKLLKEHQEELQGTVILVFQPAEEGGAGAKKIVEAGVLENVGAIFGLHVSNLLGL 201

Query: 206 GMVVSRPGPILAASGFFEAVISGKGGHAALPQHTIDPILAASNIIVSLQHLISREADPLD 265
           G + SR G ++A SG F+A ISGKGGHAALPQ  IDP+LAASN+I+SLQHL+SREADPLD
Sbjct: 202 GQLSSREGLLMAGSGRFKATISGKGGHAALPQFAIDPVLAASNVILSLQHLVSREADPLD 261

Query: 266 SQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQRIVEVITKQANVQRCNATV 325
           SQVVTVA F+G  AFNVIPDSVTIGGTFRA   +SF QLKQRIV+VIT QA+V  CNATV
Sbjct: 262 SQVVTVATFEGSDAFNVIPDSVTIGGTFRALLPKSFEQLKQRIVQVITTQASVNMCNATV 321

Query: 326 EFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKSILDMQPLMGAEDFAFYGEVIPGFFY 385
           +F ED+ P FP T+N++ L+  ++ V+ DMLG ++ ++  P+M +EDFAFY + IPG F 
Sbjct: 322 DFLEDETPPFPPTVNNKTLHLFYKNVSVDMLGIENYVETLPVMVSEDFAFYQQAIPGHFS 381

Query: 386 FVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQASIATRYLLEYS 432
           FVGM N++       HSP + VNE++LPYGA+L AS+ATRYLL+ S
Sbjct: 382 FVGMQNKSHSPMANPHSPFFEVNEELLPYGASLLASLATRYLLDSS 427

BLAST of Lag0034511 vs. TAIR 10
Match: AT5G56660.1 (IAA-leucine resistant (ILR)-like 2 )

HSP 1 Score: 543.9 bits (1400), Expect = 1.2e-154
Identity = 277/448 (61.83%), Postives = 341/448 (76.12%), Query Frame = 0

Query: 1   MPLHSWLILIFIFHVLMLWPIPSTSSPSP---EELSQIPLNFLQHAKNPEVFDWMVDIRR 60
           M L+  L L F   + +L    S SS SP   E+ SQI    L+ AK+PEVFDWMV IRR
Sbjct: 1   MALNKLLSLTFQLLLFLL----SVSSESPWIAEDTSQIQTKLLEFAKSPEVFDWMVKIRR 60

Query: 61  KIHENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDAL 120
           KIHENPELG+EE ETSKLIR+EL+ +GI YRYPVAITGV+G+IGTG PPFVA+RADMDAL
Sbjct: 61  KIHENPELGYEELETSKLIRSELELIGIKYRYPVAITGVIGYIGTGEPPFVALRADMDAL 120

Query: 121 ALQEGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGG 180
            +QEGVEW HKSK+ GKMHACGHD HV MLLGAAKIL EHR  +QGTVVL+FQPAEEG  
Sbjct: 121 PIQEGVEWEHKSKIAGKMHACGHDGHVTMLLGAAKILHEHRHHLQGTVVLIFQPAEEGLS 180

Query: 181 GAKKVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQ 240
           GAKK+ E G L NV+AIFG+H+S   PFG   SR G  LA +G FEAVI+GKGGHAA+PQ
Sbjct: 181 GAKKMREEGALKNVEAIFGIHLSARIPFGKAASRAGSFLAGAGVFEAVITGKGGHAAIPQ 240

Query: 241 HTIDPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFS 300
           HTIDP++AAS+I++SLQ L+SRE DPLDS+VVTV+K  GG AFNVIPDS+TIGGT RAF+
Sbjct: 241 HTIDPVVAASSIVLSLQQLVSRETDPLDSKVVTVSKVNGGNAFNVIPDSITIGGTLRAFT 300

Query: 301 KESFIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLG 360
              F QL+QR+ EVITKQA V RCNA+V    + +   P T+N++DLYK F+ V  D+LG
Sbjct: 301 --GFTQLQQRVKEVITKQAAVHRCNASVNLTPNGREPMPPTVNNKDLYKQFKKVVRDLLG 360

Query: 361 TKSILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGAT 420
            ++ ++  P+MG+EDF+++ E IPG F  +GM +ET G +  +HSP Y +NEDVLPYGA 
Sbjct: 361 QEAFVEAAPVMGSEDFSYFAETIPGHFSLLGMQDETNG-YASSHSPLYRINEDVLPYGAA 420

Query: 421 LQASIATRYLLEYSQKSTSTKGYSHDEL 446
           + AS+A +YL E + K  S  G+ H+EL
Sbjct: 421 IHASMAVQYLKEKASKG-SVSGF-HEEL 439

BLAST of Lag0034511 vs. TAIR 10
Match: AT5G56650.1 (IAA-leucine resistant (ILR)-like 1 )

HSP 1 Score: 536.2 bits (1380), Expect = 2.5e-152
Identity = 267/435 (61.38%), Postives = 337/435 (77.47%), Query Frame = 0

Query: 13  FHVLMLWPIPSTSSP--SPEELSQIPLNFLQHAKNPEVFDWMVDIRRKIHENPELGFEEF 72
           F +L+L    S+ SP     ++S+IP+NFL+ AK+PEVFD MV IRRKIHENPELG+EEF
Sbjct: 9   FQLLLLLLRVSSESPWIVAGDVSRIPINFLELAKSPEVFDSMVRIRRKIHENPELGYEEF 68

Query: 73  ETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNPPFVAIRADMDALALQEGVEWAHKSK 132
           ETSK IR+ELD +G+ YR+PVAITG++G+IGTG PPFVA+RADMDAL +QE VEW HKSK
Sbjct: 69  ETSKFIRSELDLIGVKYRFPVAITGIIGYIGTGEPPFVALRADMDALPIQEAVEWEHKSK 128

Query: 133 VPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTVVLVFQPAEEGGGGAKKVLEAGVLDN 192
            PGKMHACGHD HVAMLLGAAKILQ+HR+ +QGTVVL+FQPAEEG  GAK + E G L N
Sbjct: 129 NPGKMHACGHDGHVAMLLGAAKILQQHRQHLQGTVVLIFQPAEEGLSGAKMMREEGALKN 188

Query: 193 VDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAVISGKGGHAALPQHTIDPILAASNII 252
           V+AIFG+H+SP  PFG   S  G  +A +G FEAVI+GKGGHAA+PQHTIDP++AAS+I+
Sbjct: 189 VEAIFGIHLSPRTPFGKAASLAGSFMAGAGAFEAVITGKGGHAAIPQHTIDPVVAASSIV 248

Query: 253 VSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPDSVTIGGTFRAFSKESFIQLKQRIVE 312
           +SLQHL+SRE DP DS+VVTV K  GG AFNVIPDS+TIGGT RAF+   F QL++RI E
Sbjct: 249 LSLQHLVSRETDPSDSKVVTVTKVNGGNAFNVIPDSITIGGTLRAFT--GFTQLQERIKE 308

Query: 313 VITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLYKHFRAVAGDMLGTKSILDMQPLMGA 372
           +ITKQA V RCNA+V    +     P T+N+ DLYK F+ V  D+LG ++ ++  P MG+
Sbjct: 309 IITKQAAVHRCNASVNLAPNGNQPMPPTVNNMDLYKKFKKVVRDLLGQEAFVEAVPEMGS 368

Query: 373 EDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNYIVNEDVLPYGATLQASIATRYLLEY 432
           EDF+++ E IPG F  +GM +ET G +  +HSP+Y +NEDVLPYGA + A++A +YL + 
Sbjct: 369 EDFSYFAETIPGHFSLLGMQDETQG-YASSHSPHYRINEDVLPYGAAIHATMAVQYLKDK 428

Query: 433 SQKSTSTKGYSHDEL 446
           + K  S  G+ HDEL
Sbjct: 429 ASKG-SVSGF-HDEL 438

BLAST of Lag0034511 vs. TAIR 10
Match: AT1G44350.1 (IAA-leucine resistant (ILR)-like gene 6 )

HSP 1 Score: 433.7 bits (1114), Expect = 1.8e-121
Identity = 213/382 (55.76%), Postives = 271/382 (70.94%), Query Frame = 0

Query: 46  PEVFDWMVDIRRKIHENPELGFEEFETSKLIRAELDRLGISYRYPVAITGVVGFIGTGNP 105
           P+   W+  +RR IHENPEL FEE+ETS+LIR+ELDR+GI YRYP+A TG+  +IG+G P
Sbjct: 83  PDNVAWLKRVRRTIHENPELAFEEYETSRLIRSELDRMGIMYRYPLAKTGIRAWIGSGGP 142

Query: 106 PFVAIRADMDALALQEGVEWAHKSKVPGKMHACGHDAHVAMLLGAAKILQEHRRLIQGTV 165
           PFVA+RADMDAL +QE VEW H SKV GKMHACGHDAHV MLLGAA IL+    L++GTV
Sbjct: 143 PFVAVRADMDALPIQEAVEWEHISKVAGKMHACGHDAHVTMLLGAAHILKAREHLLKGTV 202

Query: 166 VLVFQPAEEGGGGAKKVLEAGVLDNVDAIFGLHVSPSYPFGMVVSRPGPILAASGFFEAV 225
           VL+FQPAEE G GAK ++E G LD+V+AIF +HVS  +P G++ SR GP+LA  G F AV
Sbjct: 203 VLLFQPAEEAGNGAKNMIEDGALDDVEAIFAVHVSHIHPTGVIGSRSGPLLAGCGIFRAV 262

Query: 226 ISGKGGHAALPQHTIDPILAASNIIVSLQHLISREADPLDSQVVTVAKFQGGGAFNVIPD 285
           I+ +    A      + +LAAS+ ++SLQ ++SREA PLDSQVV+V  F GG + +V PD
Sbjct: 263 ITSEDSRGA-----ANLLLAASSAVISLQGIVSREASPLDSQVVSVTSFDGGHSLDVAPD 322

Query: 286 SVTIGGTFRAFSKESFIQLKQRIVEVITKQANVQRCNATVEFNEDQKPFFPVTLNDQDLY 345
           +V +GGTFRAFS  SF  LK+RI EV+  Q  V  C ATV F E Q   +P T N+   Y
Sbjct: 323 TVVLGGTFRAFSNSSFYYLKKRIQEVLMDQVGVFGCQATVNFFEKQNAIYPPTTNNDATY 382

Query: 346 KHFRAVAGDMLGTKSILDMQPLMGAEDFAFYGEVIPGFFYFVGMTNETLGKFKPAHSPNY 405
            H + V  D+LG         +MGAEDFAFY E+IP  FYF+G+ NE LG    AHSP++
Sbjct: 383 NHLKKVTIDLLGDSHFTLAPQMMGAEDFAFYSEIIPAAFYFIGIRNEELGSVHIAHSPHF 442

Query: 406 IVNEDVLPYGATLQASIATRYL 428
           +++ED LP GA + A++A RYL
Sbjct: 443 MIDEDSLPVGAAVHAAVAERYL 459

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038878754.12.4e-22988.99IAA-amino acid hydrolase ILR1-like 4 [Benincasa hispida][more]
KAG6588014.12.0e-22889.66IAA-amino acid hydrolase ILR1-like 4, partial [Cucurbita argyrosperma subsp. sor... [more]
KAG7021909.15.9e-22889.44IAA-amino acid hydrolase ILR1-like 4 [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_023530622.13.2e-22689.21IAA-amino acid hydrolase ILR1-like 4 [Cucurbita pepo subsp. pepo] >XP_023530623.... [more]
XP_022933830.17.2e-22688.76IAA-amino acid hydrolase ILR1-like 4 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O043731.9e-17367.87IAA-amino acid hydrolase ILR1-like 4 OS=Arabidopsis thaliana OX=3702 GN=ILL4 PE=... [more]
Q84XG91.3e-16669.59IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. indica OX=39946 GN=I... [more]
Q8S9S45.1e-16669.34IAA-amino acid hydrolase ILR1-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q9SWX91.2e-15465.76IAA-amino acid hydrolase ILR1-like 5 OS=Arabidopsis thaliana OX=3702 GN=ILL5 PE=... [more]
P549701.7e-15361.83IAA-amino acid hydrolase ILR1-like 2 OS=Arabidopsis thaliana OX=3702 GN=ILL2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1F5X73.5e-22688.76IAA-amino acid hydrolase ILR1-like 4 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5D3CIQ73.3e-22487.87IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BQ363.3e-22487.87IAA-amino acid hydrolase ILR1-like 4 OS=Cucumis melo OX=3656 GN=LOC103492283 PE=... [more]
A0A0A0LYS15.8e-22186.74M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G575150 P... [more]
A0A6J1KT141.1e-21987.61IAA-amino acid hydrolase ILR1-like 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G51760.11.4e-17467.87peptidase M20/M25/M40 family protein [more]
AT1G51780.18.4e-15665.76IAA-leucine resistant (ILR)-like gene 5 [more]
AT5G56660.11.2e-15461.83IAA-leucine resistant (ILR)-like 2 [more]
AT5G56650.12.5e-15261.38IAA-leucine resistant (ILR)-like 1 [more]
AT1G44350.11.8e-12155.76IAA-leucine resistant (ILR)-like gene 6 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017439AmidohydrolasePIRSFPIRSF005962Amidohydrol_AmhXcoord: 37..430
e-value: 8.7E-130
score: 431.0
IPR017439AmidohydrolaseTIGRFAMTIGR01891TIGR01891coord: 52..414
e-value: 1.0E-115
score: 384.8
IPR017439AmidohydrolasePANTHERPTHR11014PEPTIDASE M20 FAMILY MEMBERcoord: 1..445
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 109..422
e-value: 7.5E-35
score: 120.8
IPR011650Peptidase M20, dimerisation domainPFAMPF07687M20_dimercoord: 222..316
e-value: 6.4E-11
score: 42.1
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 52..412
e-value: 3.2E-142
score: 475.6
NoneNo IPR availableGENE3D3.30.70.360coord: 217..335
e-value: 3.2E-142
score: 475.6
NoneNo IPR availablePANTHERPTHR11014:SF119IAA-AMINO ACID HYDROLASE ILR1-LIKE 4coord: 1..445
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 47..428
IPR044757IAA-amino acid hydrolase ILR1-likeCDDcd08017M20_IAA_Hydcoord: 52..427
e-value: 0.0
score: 648.61
IPR036264Bacterial exopeptidase dimerisation domainSUPERFAMILY55031Bacterial exopeptidase dimerisation domaincoord: 217..336

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0034511.1Lag0034511.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009850 auxin metabolic process
molecular_function GO:0010179 IAA-Ala conjugate hydrolase activity
molecular_function GO:0016787 hydrolase activity