Lag0033972 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033972
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein DETOXIFICATION
Locationchr3: 3390330 .. 3393461 (-)
RNA-Seq ExpressionLag0033972
SyntenyLag0033972
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGAGATCAGAACTCGTCGTTGAACTCATCCCTTCTTCACATATCTGAAGATGAAGTGAATTTGAAAGACGAGAGACTAGCAGATGATAAGAATTTCAGAAGGAAACTCATAGCCGAGGAAGTTAAGAAGCAGCTATGGCTAGCAGGGCCTCTAATATCGGTCAGTCTTCTACAATACTGTTTGCAGATGATTTCCATCATGTTTGTGGGTCATCTCGGCGAATTGCCTCTCTCCGGTGCTTCCATGGCTGTTTCTTTTACATCGGTGACTGGTTTCACCGTCTTGGTGAGTTGGTTTTCTGTTTGTTTCTTGGGGAAAGTTACATCTTCTTAGTAATCTGTCCGATTTTATAAGACAATAATTTGTTATTGCTTAGCATCATAGAGCGAACTAGGAGACTGTGAATTTGTTGGTTTAGGAATCTGCAACTTTCATTCCCATATGTAACAGATATTTAATCATGGTTTTATTGAGAATGATGCACTTTGTTTCTAAATCTAGTCATCTGACTTCAAATGGTCTTGATTGGAATACATGTCTAGCTGGGGATGGCTAGTGCTCTGGATACGTTTTGTGGCCAATCTTATGGAGCAAAGCAGTACCACATGTTGGGCATCCATATGCAAAGAGCAATGTTTGTTCTTTTACTTTTGAGCATACCTCTTGCAGTCATCTGGGCTAACACGGGAGAAATCCTGCAATTAGTTGGCCAAGATGCTCAAATTTCAGCAGAAGCTGGGAAATATGCTAAATGCTTGATACCATGCCTTTTTGCACATGGTCTTGTTCAGTGCCTGAACAGATTTTTACAGACCCAAAACGTTGTTTTCCCAATGATGGTGAGTTCTGGAATAGCAGCTTTGCTTCACATCCTCCTCTGTTGGGTTATGGTATTCAAAGCTGGGCTTGAAACTCGAGGAGCTGCTTTGGCAAACTCCATTTCTTATTGGATCAATGCATTGATATTGATACTTTATGTCAAGTTTTCTTCTTCATGTTCGAAGTCTTGGACTGGCTTTTCAGCGCAGGCTTTTCACAACATCCCATATTTCCTTAAACTTGCAATCCCTTCAACTGTGATGGTTTGGTAAAAATCACTTTTACTGTTTCTGCTTTTATCATAATCACAAAGATGCAGTTCTCTCACAATGTTCAAAAAGATTTTCAACAGCCTAGAACTGTGGTCATTCGAGATGGTGGTTCTTCTGTCTGGACTTCTACCAAATCCAAAGTTAGAGACATCAGTGCTTTCTATTAGGTATAAACTAGAGACATCAGTTCTTCAAGAGCAAGTTCCTTTTCTTTCTTTTTTATCTAACCACTTTTAGCTTTACCAACACATTTCTTTACAAGCCTTAATACAGATTTAACCGTTTGGATGATCCCAATGGGTCTGAGTGCAGCTGCTAGGTAAATTTGCCACTATTTCTCATAAGAATCTCCTTTTTCTATAACGATAACTTAAAAATGATCACTTTCATGTCTTTCTCCATCTTGCTTAAATTGGGAAAGTGAGAACCTTGCATTTTTTTTTTTATATGAATGGATATAATTCAAATTTAACTAAGATTTCTGTCCTTACTTCATTTCATAAGCACGCGAGTTTCAAATGAACTAGGAGCTGGTCGTCCTGCAGCAGCAAAGCTAGCAGCAAGTATAGTTATGACAATAGCCGTTATTGAGGGGCTACTGGTTGCAACTGTCTTGGTTCTTATTCGAAATGTTTGGGGTTATGCGTATAGCAATGAACCAGAAGTGGTCAAATATGTAGCGGACGTGGTTCCCATAATTGCGGTGTCCAGTTTTCTCGATGGACTTCAATGTGTTCTTTCAGGTTCTTACTTTATAATTATTTACAATCATATATTTCTTCTCTGTATGCATTTCATTTCATTTGACTCCAGCTACCTTTCAATTGGAAATTATAGAGTTGGAATCGAATGCTGCTATTTATGACTGTTGGGAGACCAAGTTCTAATTCAAAGATTGGACTCTTTTGCTGTAATTAGCTCTTGTTTCTTTTGGAAACGATGATTGTTTGAACCAACTTGCATGCACTAATCTTACTGGATAACACATAAAAACTCATGAGATATCTGAAATCTTCGATAGGTGGCCATCATAGTTGAATTCATTACCATATGCCAATGAAGGTCATAATTTACATGGTATCTTTTTGACTGGTAGGCAACCTGTTGTGGTTGTGGTTAGCCCTGCCAAGTTGTCTAGATGACACTTTCTAGTGGAGTACTAAGACGTGAAATGGAAATGAAAGAGGAATTCTATGCCTATCAAAAAGTGCCATTTATTTCAAGTTAGGTCATAAGAAATCAAACATAATCTCTTTGAAACTCTAGTTTCAGCCAAAAGAAAGGGGCAAAGTTTTTACAGAATATTATATTTTTAAGGTAAACCTTGATAAATAGTATGCAATAAAATGAATTTGACCTGTCTGGCTGTTTCCTTTGGACATGAGTAAATTGCTAAATCTCTCTGTTCTTCATTTTGTTAATATTAGACTTGGGATTATATTTGTACAGTCGCAAATAACTTGATCCTTTTGTCTGTACAATATTTTTCTATCCCTTGTGATCTAACCAGACACATTTACCTGCATAAAGTATTGGTATTTTGAAGAATCTGTTTGTATGTTCTTAAATAAAATGCAGGCATTGCTAGAGGATGTGGTTGGCAGAAAATTGGTGCTTATGTCAATCTTGGATCATATTATCTTGTGGGAATCCCATCTGCAGTTCTGCTTGCTTTTGTCATGCACGTTGGGGGAAAGGCAAAAATGATAATCATCTCCACTCTTCATGCTCACCATTTTACTTTATGTCCTATATTATTACTAATCCATTTTTTAACATTTTTCTCTTGAAAATGATCCTCAATAGGGGCTGTGGTTAGGCATCATCTGTGCACTTGCTGTCCAAACACTTTCTCTTGCTATAATTACCATCCGCACCAACTGGGACCAAGAAGTACAACTCTAAACTCAAATCTCAACTTTCCTTGATTCCCTTGAAATTTGATAGTCCTGATGCACTCATTCATCATATTCAGGCAAAGATTGCTACAGAACGAGTTTACAATGCAGTAATTCCAACAAATGCCATCTCATGA

mRNA sequence

ATGGCGAGAGATCAGAACTCGTCGTTGAACTCATCCCTTCTTCACATATCTGAAGATGAAGTGAATTTGAAAGACGAGAGACTAGCAGATGATAAGAATTTCAGAAGGAAACTCATAGCCGAGGAAGTTAAGAAGCAGCTATGGCTAGCAGGGCCTCTAATATCGGTCAGTCTTCTACAATACTGTTTGCAGATGATTTCCATCATGTTTGTGGGTCATCTCGGCGAATTGCCTCTCTCCGGTGCTTCCATGGCTGTTTCTTTTACATCGGTGACTGGTTTCACCGTCTTGCTGGGGATGGCTAGTGCTCTGGATACGTTTTGTGGCCAATCTTATGGAGCAAAGCAGTACCACATGTTGGGCATCCATATGCAAAGAGCAATGTTTGTTCTTTTACTTTTGAGCATACCTCTTGCAGTCATCTGGGCTAACACGGGAGAAATCCTGCAATTAGTTGGCCAAGATGCTCAAATTTCAGCAGAAGCTGGGAAATATGCTAAATGCTTGATACCATGCCTTTTTGCACATGGTCTTGTTCAGTGCCTGAACAGATTTTTACAGACCCAAAACGTTGTTTTCCCAATGATGGTGAGTTCTGGAATAGCAGCTTTGCTTCACATCCTCCTCTGTTGGGTTATGGTATTCAAAGCTGGGCTTGAAACTCGAGGAGCTGCTTTGGCAAACTCCATTTCTTATTGGATCAATGCATTGATATTGATACTTTATGTCAAGTTTTCTTCTTCATGTTCGAAGTCTTGGACTGGCTTTTCAGCGCAGGCTTTTCACAACATCCCATATTTCCTTAAACTTGCAATCCCTTCAACTGTGATGGTTTGCCTAGAACTGTGGTCATTCGAGATGGTGGTTCTTCTGTCTGGACTTCTACCAAATCCAAAGTTAGAGACATCAGTGCTTTCTATTAGCCTTAATACAGATTTAACCGTTTGGATGATCCCAATGGGTCTGAGTGCAGCTGCTAGCACGCGAGTTTCAAATGAACTAGGAGCTGGTCGTCCTGCAGCAGCAAAGCTAGCAGCAAGTATAGTTATGACAATAGCCGTTATTGAGGGGCTACTGGTTGCAACTGTCTTGGTTCTTATTCGAAATGTTTGGGGTTATGCGTATAGCAATGAACCAGAAGTGGTCAAATATGTAGCGGACGTGGTTCCCATAATTGCGGTGTCCAGTTTTCTCGATGGACTTCAATGTGTTCTTTCAGGCATTGCTAGAGGATGTGGTTGGCAGAAAATTGGTGCTTATGTCAATCTTGGATCATATTATCTTGTGGGAATCCCATCTGCAGTTCTGCTTGCTTTTGTCATGCACGTTGGGGGAAAGGGGCTGTGGTTAGGCATCATCTGTGCACTTGCTGTCCAAACACTTTCTCTTGCTATAATTACCATCCGCACCAACTGGGACCAAGAAGCAAAGATTGCTACAGAACGAGTTTACAATGCAGTAATTCCAACAAATGCCATCTCATGA

Coding sequence (CDS)

ATGGCGAGAGATCAGAACTCGTCGTTGAACTCATCCCTTCTTCACATATCTGAAGATGAAGTGAATTTGAAAGACGAGAGACTAGCAGATGATAAGAATTTCAGAAGGAAACTCATAGCCGAGGAAGTTAAGAAGCAGCTATGGCTAGCAGGGCCTCTAATATCGGTCAGTCTTCTACAATACTGTTTGCAGATGATTTCCATCATGTTTGTGGGTCATCTCGGCGAATTGCCTCTCTCCGGTGCTTCCATGGCTGTTTCTTTTACATCGGTGACTGGTTTCACCGTCTTGCTGGGGATGGCTAGTGCTCTGGATACGTTTTGTGGCCAATCTTATGGAGCAAAGCAGTACCACATGTTGGGCATCCATATGCAAAGAGCAATGTTTGTTCTTTTACTTTTGAGCATACCTCTTGCAGTCATCTGGGCTAACACGGGAGAAATCCTGCAATTAGTTGGCCAAGATGCTCAAATTTCAGCAGAAGCTGGGAAATATGCTAAATGCTTGATACCATGCCTTTTTGCACATGGTCTTGTTCAGTGCCTGAACAGATTTTTACAGACCCAAAACGTTGTTTTCCCAATGATGGTGAGTTCTGGAATAGCAGCTTTGCTTCACATCCTCCTCTGTTGGGTTATGGTATTCAAAGCTGGGCTTGAAACTCGAGGAGCTGCTTTGGCAAACTCCATTTCTTATTGGATCAATGCATTGATATTGATACTTTATGTCAAGTTTTCTTCTTCATGTTCGAAGTCTTGGACTGGCTTTTCAGCGCAGGCTTTTCACAACATCCCATATTTCCTTAAACTTGCAATCCCTTCAACTGTGATGGTTTGCCTAGAACTGTGGTCATTCGAGATGGTGGTTCTTCTGTCTGGACTTCTACCAAATCCAAAGTTAGAGACATCAGTGCTTTCTATTAGCCTTAATACAGATTTAACCGTTTGGATGATCCCAATGGGTCTGAGTGCAGCTGCTAGCACGCGAGTTTCAAATGAACTAGGAGCTGGTCGTCCTGCAGCAGCAAAGCTAGCAGCAAGTATAGTTATGACAATAGCCGTTATTGAGGGGCTACTGGTTGCAACTGTCTTGGTTCTTATTCGAAATGTTTGGGGTTATGCGTATAGCAATGAACCAGAAGTGGTCAAATATGTAGCGGACGTGGTTCCCATAATTGCGGTGTCCAGTTTTCTCGATGGACTTCAATGTGTTCTTTCAGGCATTGCTAGAGGATGTGGTTGGCAGAAAATTGGTGCTTATGTCAATCTTGGATCATATTATCTTGTGGGAATCCCATCTGCAGTTCTGCTTGCTTTTGTCATGCACGTTGGGGGAAAGGGGCTGTGGTTAGGCATCATCTGTGCACTTGCTGTCCAAACACTTTCTCTTGCTATAATTACCATCCGCACCAACTGGGACCAAGAAGCAAAGATTGCTACAGAACGAGTTTACAATGCAGTAATTCCAACAAATGCCATCTCATGA

Protein sequence

MARDQNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIATERVYNAVIPTNAIS
Homology
BLAST of Lag0033972 vs. NCBI nr
Match: XP_023531831.1 (protein DETOXIFICATION 16-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 870.2 bits (2247), Expect = 8.8e-249
Identity = 450/494 (91.09%), Postives = 471/494 (95.34%), Query Frame = 0

Query: 1   MARDQNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           M RDQ S LNS L+HISEDEVN KD RL DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQKSWLNSPLIHISEDEVNPKDGRLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAM +LLL+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMVILLLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAALLHI LCWVM+FKAGLETRGAALANSISYWINALILI
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHNI YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA +VMTIAVIEGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVIEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNE EVVKYVAD+VPIIAVS+F DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIAT 480
           VNLGSYYLVGIPSAVLLAFV+H+GGKGLWLGII ALAVQTLSLAIITIRTNWD+EAKIA 
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIGGKGLWLGIISALAVQTLSLAIITIRTNWDEEAKIAA 480

Query: 481 ERVYNAVIPTNAIS 495
           ERVY +VIPTN I+
Sbjct: 481 ERVYESVIPTNVIA 494

BLAST of Lag0033972 vs. NCBI nr
Match: XP_023005095.1 (protein DETOXIFICATION 16-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 869.8 bits (2246), Expect = 1.1e-248
Identity = 450/494 (91.09%), Postives = 470/494 (95.14%), Query Frame = 0

Query: 1   MARDQNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           M RDQ S L S L+HISEDEVNLKD  L DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQESWLKSPLIHISEDEVNLKDGGLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAALLHI LCWVM+FKAGLETRGAALANSISYWINALILI
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHNI YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA +VMTIAVIEGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVIEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNE EVVKYVAD+VPIIAVS+F DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIAT 480
           VNLGSYYLVGIPSAVLLAFV+H+ GKGLWLGIICALAVQTLSLAIITIRTNWD+EAKIA 
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIDGKGLWLGIICALAVQTLSLAIITIRTNWDEEAKIAA 480

Query: 481 ERVYNAVIPTNAIS 495
           ERVY +VIPTN I+
Sbjct: 481 ERVYESVIPTNVIA 494

BLAST of Lag0033972 vs. NCBI nr
Match: XP_022929082.1 (protein DETOXIFICATION 16-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 866.7 bits (2238), Expect = 9.7e-248
Identity = 448/494 (90.69%), Postives = 469/494 (94.94%), Query Frame = 0

Query: 1   MARDQNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           M RDQ S LNS L+HISEDEVN KD RL DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQKSWLNSPLIHISEDEVNPKDGRLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAALLHI LCWVM+FKAGLETRGAALANSISYWINALIL+
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILM 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHN  YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNFSYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA +VMTIAV EGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVFEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNE EVVKYVAD+VPIIAVS+F DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIAT 480
           VNLGSYYLVGIPSAVLLAFV+H+GGKGLWLGII ALAVQTLSLAIITIRTNWD+EAKIA 
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIGGKGLWLGIISALAVQTLSLAIITIRTNWDEEAKIAA 480

Query: 481 ERVYNAVIPTNAIS 495
           ERVY +VIPTN I+
Sbjct: 481 ERVYESVIPTNVIA 494

BLAST of Lag0033972 vs. NCBI nr
Match: KAG6587669.1 (Protein DETOXIFICATION 16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 862.8 bits (2228), Expect = 1.4e-246
Identity = 447/494 (90.49%), Postives = 469/494 (94.94%), Query Frame = 0

Query: 1   MARDQNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           M RDQ S LNS L+HISEDEVN KD RL DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQKSWLNSPLIHISEDEVNPKDGRLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAALLHI LCWVM+FKAGLETRGAALANSISYWINALILI
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHNI YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA +VMTIAVIEGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVIEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNE EVVKYVAD++PIIAVS+F DGLQCVLSGI RG GWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIIPIIAVSTFFDGLQCVLSGIVRGSGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIAT 480
           VNLGSYYLVGIPSAVLLAFV+H+GGKGLWLGII ALAVQTLSLAIITIRTNW +EAKIA 
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIGGKGLWLGIISALAVQTLSLAIITIRTNWVEEAKIAA 480

Query: 481 ERVYNAVIPTNAIS 495
           ERVY +VIPTN ++
Sbjct: 481 ERVYESVIPTNVVA 494

BLAST of Lag0033972 vs. NCBI nr
Match: XP_004137332.2 (protein DETOXIFICATION 16 [Cucumis sativus])

HSP 1 Score: 854.4 bits (2206), Expect = 5.0e-244
Identity = 447/490 (91.22%), Postives = 466/490 (95.10%), Query Frame = 0

Query: 5   QNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQ 64
           QNSS NS L+H SEDEVN KDER  D +N RRKLIAEEVKKQLWLAGPLI VSLLQYCLQ
Sbjct: 7   QNSSFNSPLIHKSEDEVNSKDERQIDYENIRRKLIAEEVKKQLWLAGPLILVSLLQYCLQ 66

Query: 65  MISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHM 124
           MISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIH+
Sbjct: 67  MISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHL 126

Query: 125 QRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNR 184
           QRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQCL R
Sbjct: 127 QRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQCLYR 186

Query: 185 FLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVK 244
           FLQTQNVVFPMM+SSGI+AL HI LCW+MVFK GL TRGAA+ANSISYWINALILILYVK
Sbjct: 187 FLQTQNVVFPMMMSSGISALSHITLCWLMVFKVGLGTRGAAVANSISYWINALILILYVK 246

Query: 245 FSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSV 304
           FSSS SK+WTGFS  AFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSV
Sbjct: 247 FSSSSSKTWTGFSRLAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSV 306

Query: 305 LSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVL 364
           LSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLA  +VMTIAVIEGLL+ATVL
Sbjct: 307 LSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAGYVVMTIAVIEGLLLATVL 366

Query: 365 VLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLG 424
           +L+RNVWGYAYSNE EVV+YVA+VVP+IAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLG
Sbjct: 367 ILVRNVWGYAYSNEQEVVEYVANVVPLIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLG 426

Query: 425 SYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIATERVY 484
           SYYLVGIPSAVLLAFV HVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAK ATERVY
Sbjct: 427 SYYLVGIPSAVLLAFVFHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKKATERVY 486

Query: 485 NAVIPTNAIS 495
           +AVIP NAIS
Sbjct: 487 DAVIPVNAIS 496

BLAST of Lag0033972 vs. ExPASy Swiss-Prot
Match: Q9FHB6 (Protein DETOXIFICATION 16 OS=Arabidopsis thaliana OX=3702 GN=DTX16 PE=2 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 2.1e-173
Identity = 303/445 (68.09%), Postives = 367/445 (82.47%), Query Frame = 0

Query: 39  IAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLL 98
           + EEVKKQLWL+GPLI+VSLLQ+CLQ+IS+MFVGHLG LPLS AS+A SF SVTGF+ L+
Sbjct: 23  VKEEVKKQLWLSGPLIAVSLLQFCLQVISVMFVGHLGSLPLSAASIATSFASVTGFSFLM 82

Query: 99  GMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQI 158
           G ASALDT CGQ+YGAK+Y MLGI MQRAMFVL L SIPL++IWANT  +L   GQ+  I
Sbjct: 83  GTASALDTLCGQAYGAKKYGMLGIQMQRAMFVLTLASIPLSIIWANTEHLLVFFGQNKSI 142

Query: 159 SAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAG 218
           +  AG YAK +IP +FA+GL+QC NRFLQ QN VFP++  SG+   LH+LLCWV+VFK+G
Sbjct: 143 ATLAGSYAKFMIPSIFAYGLLQCFNRFLQAQNNVFPVVFCSGVTTSLHVLLCWVLVFKSG 202

Query: 219 LETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMV 278
           L  +GAALANSISYW+N ++L  YVKFS SCS +WTGFS +A  +I  FL+LA+PS +MV
Sbjct: 203 LGFQGAALANSISYWLNVVLLFCYVKFSPSCSLTWTGFSKEALRDILPFLRLAVPSALMV 262

Query: 279 CLELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGR 338
           CLE+WSFE++VLLSGLLPNP LETSVLSI LNT  T+WMIP GLS AASTR+SNELGAG 
Sbjct: 263 CLEMWSFELLVLLSGLLPNPVLETSVLSICLNTSGTMWMIPFGLSGAASTRISNELGAGN 322

Query: 339 PAAAKLAASIVMTIAVIEGLLVATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFL 398
           P  AKLA  +V+ IAV E +++ +VL+LIRN+WG AYS+E EVV YVA ++PI+A+ +FL
Sbjct: 323 PKVAKLAVRVVICIAVAESIVIGSVLILIRNIWGLAYSSELEVVSYVASMMPILALGNFL 382

Query: 399 DGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAV 458
           D LQCVLSG+ARGCGWQKIGA +NLGSYYLVG+PS +LLAF  HVGG+GLWLGIICAL V
Sbjct: 383 DSLQCVLSGVARGCGWQKIGAIINLGSYYLVGVPSGLLLAFHFHVGGRGLWLGIICALVV 442

Query: 459 QTLSLAIITIRTNWDQEAKIATERV 484
           Q   L ++TI TNWD+EAK AT R+
Sbjct: 443 QVFGLGLVTIFTNWDEEAKKATNRI 467

BLAST of Lag0033972 vs. ExPASy Swiss-Prot
Match: Q9C9U1 (Protein DETOXIFICATION 17 OS=Arabidopsis thaliana OX=3702 GN=DTX17 PE=2 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.5e-166
Identity = 299/456 (65.57%), Postives = 361/456 (79.17%), Query Frame = 0

Query: 28  LADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVS 87
           L  +K+     + EEVKKQLWL+ PLI VSLLQY LQ+IS+MFVGHLG LPLS AS+A S
Sbjct: 10  LITEKDTTMIRVKEEVKKQLWLSAPLIGVSLLQYSLQVISVMFVGHLGSLPLSAASIATS 69

Query: 88  FTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGE 147
           F SVTGFT LLG ASAL+T CGQ+YGAK Y  LGI MQRAMFVLL+LS+PL++IWANT +
Sbjct: 70  FASVTGFTFLLGTASALETLCGQAYGAKLYGKLGIQMQRAMFVLLILSVPLSIIWANTEQ 129

Query: 148 ILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAALLHI 207
           IL LV QD  I++ AG YAK +IP LFA+GL+QC+NRFLQ QN VFP+ V SGI   LH+
Sbjct: 130 ILVLVHQDKSIASVAGSYAKYMIPSLFAYGLLQCINRFLQAQNNVFPVFVCSGITTCLHL 189

Query: 208 LLCWVMVFKAGLETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYF 267
           LLCW+ V K GL  RGAALA S+SYW N ++L  YVKFS SCS SWTGFS +AF  +  F
Sbjct: 190 LLCWLFVLKTGLGYRGAALAISVSYWFNVILLSCYVKFSPSCSHSWTGFSKEAFQELYDF 249

Query: 268 LKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAAS 327
            K+A PS VMVCLELWSFE++VL SGLLPNP LETSVLSI LNT LT+W I +GL  AAS
Sbjct: 250 SKIAFPSAVMVCLELWSFELLVLASGLLPNPVLETSVLSICLNTSLTIWQISVGLGGAAS 309

Query: 328 TRVSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVLVLIRNVWGYAYSNEPEVVKYVAD 387
            RVSNELGAG P  AKLA  +++ IAV EG++V TVL+ IR + G+A+S++P+++ Y A 
Sbjct: 310 IRVSNELGAGNPQVAKLAVYVIVGIAVAEGIVVVTVLLSIRKILGHAFSSDPKIIAYAAS 369

Query: 388 VVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVMHVGGKG 447
           ++PI+A  +FLDGLQCVLSG+ARGCGWQKIGA VNLGSYYLVG+P  +LL F  H+GG+G
Sbjct: 370 MIPIVACGNFLDGLQCVLSGVARGCGWQKIGACVNLGSYYLVGVPLGLLLGFHFHIGGRG 429

Query: 448 LWLGIICALAVQTLSLAIITIRTNWDQEAKIATERV 484
           LWLGI+ AL+VQ L L+++TI TNWD+EAK AT RV
Sbjct: 430 LWLGIVTALSVQVLCLSLVTIFTNWDKEAKKATNRV 465

BLAST of Lag0033972 vs. ExPASy Swiss-Prot
Match: F4IHU9 (Protein DETOXIFICATION 15 OS=Arabidopsis thaliana OX=3702 GN=DTX15 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 1.5e-158
Identity = 285/466 (61.16%), Postives = 359/466 (77.04%), Query Frame = 0

Query: 18  EDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGEL 77
           E+  ++    L  +K  R + + EEV+KQL L+GPLI+VSLLQ+CLQ+IS+MFVGHLG L
Sbjct: 3   EEREDMLSWPLIGEKEKRSRFVKEEVEKQLLLSGPLIAVSLLQFCLQIISVMFVGHLGSL 62

Query: 78  PLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIP 137
           PLS AS+A SF SVTGFT L+G ASA+DT CGQSYGAK Y MLGI MQRAM VL LLS+P
Sbjct: 63  PLSAASIATSFASVTGFTFLMGTASAMDTVCGQSYGAKMYGMLGIQMQRAMLVLTLLSVP 122

Query: 138 LAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMV 197
           L+++WANT   L   GQD  I+  +G YA+ +IP +FA+GL+QCLNRFLQ QN V P+++
Sbjct: 123 LSIVWANTEHFLVFFGQDKSIAHLSGSYARFMIPSIFAYGLLQCLNRFLQAQNNVIPVVI 182

Query: 198 SSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVKFSSSCSKSWTGFS 257
            SG+   LH+++CWV+V K+GL  RGAA+AN+ISYW+N ++L  YVKFS SCS +WTGFS
Sbjct: 183 CSGVTTSLHVIICWVLVLKSGLGFRGAAVANAISYWLNVILLSCYVKFSPSCSLTWTGFS 242

Query: 258 AQAFHNIPYFLKLAIPSTVMVC-LELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVW 317
            +A  +I  F+KL IPS  MVC LE+WSFE++VL SGLLPNP LETS          TVW
Sbjct: 243 KEARRDIIPFMKLVIPSAFMVCSLEMWSFELLVLSSGLLPNPVLETS-------CPRTVW 302

Query: 318 MIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVLVLIRNVWGYAYS 377
           MIP GLS AASTRVSNELG+G P  AKLA  +V++ +++E +LV TVL+LIR +WG+AYS
Sbjct: 303 MIPFGLSGAASTRVSNELGSGNPKGAKLAVRVVLSFSIVESILVGTVLILIRKIWGFAYS 362

Query: 378 NEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVL 437
           ++PEVV +VA ++PI+A+   LD  Q VLSG+ARGCGWQKIGA+VNLGSYYLVG+P  +L
Sbjct: 363 SDPEVVSHVASMLPILALGHSLDSFQTVLSGVARGCGWQKIGAFVNLGSYYLVGVPFGLL 422

Query: 438 LAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIATER 483
           L F  HVGG+GLWLGIICAL VQ + L++IT  TNWD+E K AT R
Sbjct: 423 LGFHFHVGGRGLWLGIICALIVQGVCLSLITFFTNWDEEVKKATSR 461

BLAST of Lag0033972 vs. ExPASy Swiss-Prot
Match: Q9C994 (Protein DETOXIFICATION 14 OS=Arabidopsis thaliana OX=3702 GN=DTX14 PE=1 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 3.8e-122
Identity = 239/477 (50.10%), Postives = 322/477 (67.51%), Query Frame = 0

Query: 8   SLNSSLLHISE-DEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMI 67
           S    LL +S+ +EVN KD  L             E KK  ++AGP+I+V+   Y LQ+I
Sbjct: 3   SAEKGLLVVSDREEVNKKDGFL------------RETKKLSYIAGPMIAVNSSMYVLQVI 62

Query: 68  SIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQR 127
           SIM VGHLGEL LS  ++AVSF SVTGF+V+ G+ASAL+T CGQ+ GAKQY  LG+H   
Sbjct: 63  SIMMVGHLGELFLSSTAIAVSFCSVTGFSVVFGLASALETLCGQANGAKQYEKLGVHTYT 122

Query: 128 AMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFL 187
            +  L L+ IPL+++W   G+IL L+GQDA ++ EAGK+A  LIP LF +  +Q L RF 
Sbjct: 123 GIVSLFLVCIPLSLLWTYIGDILSLIGQDAMVAQEAGKFATWLIPALFGYATLQPLVRFF 182

Query: 188 QTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVKFS 247
           Q Q+++ P+++SS  +  +HI+LCW +VFK GL + GAA+A  +SYW+N  +L LY+ FS
Sbjct: 183 QAQSLILPLVMSSVSSLCIHIVLCWSLVFKFGLGSLGAAIAIGVSYWLNVTVLGLYMTFS 242

Query: 248 SSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSVLS 307
           SSCSKS    S   F  +  F +  IPS  M+CLE WSFE +VLLSG+LPNPKLE SVLS
Sbjct: 243 SSCSKSRATISMSLFEGMGEFFRFGIPSASMICLEWWSFEFLVLLSGILPNPKLEASVLS 302

Query: 308 ISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVLVL 367
           + L+T  +++ IP  L AAASTRV+NELGAG P  A++A    M I  +E ++V  ++  
Sbjct: 303 VCLSTQSSLYQIPESLGAAASTRVANELGAGNPKQARMAVYTAMVITGVESIMVGAIVFG 362

Query: 368 IRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSY 427
            RNV+GY +S+E EVV YV  + P++++S   D L   LSG+ARG G Q IGAYVNL +Y
Sbjct: 363 ARNVFGYLFSSETEVVDYVKSMAPLLSLSVIFDALHAALSGVARGSGRQDIGAYVNLAAY 422

Query: 428 YLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIATERV 484
           YL GIP+A+LLAF   + G+GLW+GI     VQ + L +I I TNW ++A+ A ERV
Sbjct: 423 YLFGIPTAILLAFGFKMRGRGLWIGITVGSCVQAVLLGLIVILTNWKKQARKARERV 467

BLAST of Lag0033972 vs. ExPASy Swiss-Prot
Match: Q8L731 (Protein DETOXIFICATION 12 OS=Arabidopsis thaliana OX=3702 GN=DTX12 PE=2 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.4e-116
Identity = 213/442 (48.19%), Postives = 316/442 (71.49%), Query Frame = 0

Query: 42  EVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMA 101
           E+K+ ++ A P+ +V + Q+ LQ++S+M VGHLG L L+ AS+A SF +VTGF+ ++G++
Sbjct: 34  ELKRLIFFAAPMAAVVIAQFMLQIVSMMMVGHLGNLSLASASLASSFCNVTGFSFIIGLS 93

Query: 102 SALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAE 161
            ALDT  GQ+YGAK Y  LG+    AMF L L+ +PL++IW N  ++L ++GQD  I+ E
Sbjct: 94  CALDTLSGQAYGAKLYRKLGVQTYTAMFCLALVCLPLSLIWFNMEKLLLILGQDPSIAHE 153

Query: 162 AGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLET 221
           AGKYA  LIP LFA+ ++Q L R+ Q Q+++ P++++S +   +H+ LCW +V+ +GL  
Sbjct: 154 AGKYATWLIPGLFAYAVLQPLTRYFQNQSLITPLLITSYVVFCIHVPLCWFLVYNSGLGN 213

Query: 222 RGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLE 281
            G ALA S+S W+ A+ L  ++ +SS+CS++    S + F  I  F K A+PS  M+CLE
Sbjct: 214 LGGALAISLSNWLYAIFLGSFMYYSSACSETRAPLSMEIFDGIGEFFKYALPSAAMICLE 273

Query: 282 LWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAA 341
            WS+E+++LLSGLLPNP+LETSVLS+ L T  T++ IP+ ++AAASTR+SNELGAG   A
Sbjct: 274 WWSYELIILLSGLLPNPQLETSVLSVCLQTISTMYSIPLAIAAAASTRISNELGAGNSRA 333

Query: 342 AKLAASIVMTIAVIEGLLVATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGL 401
           A +     M++AVI+ L+V+  L++ RN++G+ +S++ E + YVA + P++++S  LD L
Sbjct: 334 AHIVVYAAMSLAVIDALIVSMSLLIGRNLFGHIFSSDKETIDYVAKMAPLVSISLMLDAL 393

Query: 402 QCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTL 461
           Q VLSGIARGCGWQ IGAY+NLG++YL GIP A  LAF +H+ G GLW+GI     +QTL
Sbjct: 394 QGVLSGIARGCGWQHIGAYINLGAFYLWGIPIAASLAFWIHLKGVGLWIGIQAGAVLQTL 453

Query: 462 SLAIITIRTNWDQEAKIATERV 484
            LA++T  TNW+ +A  A  R+
Sbjct: 454 LLALVTGCTNWESQADKARNRM 475

BLAST of Lag0033972 vs. ExPASy TrEMBL
Match: A0A6J1KWH9 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498176 PE=3 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 5.6e-249
Identity = 450/494 (91.09%), Postives = 470/494 (95.14%), Query Frame = 0

Query: 1   MARDQNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           M RDQ S L S L+HISEDEVNLKD  L DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQESWLKSPLIHISEDEVNLKDGGLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAALLHI LCWVM+FKAGLETRGAALANSISYWINALILI
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHNI YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA +VMTIAVIEGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVIEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNE EVVKYVAD+VPIIAVS+F DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIAT 480
           VNLGSYYLVGIPSAVLLAFV+H+ GKGLWLGIICALAVQTLSLAIITIRTNWD+EAKIA 
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIDGKGLWLGIICALAVQTLSLAIITIRTNWDEEAKIAA 480

Query: 481 ERVYNAVIPTNAIS 495
           ERVY +VIPTN I+
Sbjct: 481 ERVYESVIPTNVIA 494

BLAST of Lag0033972 vs. ExPASy TrEMBL
Match: A0A6J1ELR8 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111435773 PE=3 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.7e-248
Identity = 448/494 (90.69%), Postives = 469/494 (94.94%), Query Frame = 0

Query: 1   MARDQNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           M RDQ S LNS L+HISEDEVN KD RL DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQKSWLNSPLIHISEDEVNPKDGRLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAALLHI LCWVM+FKAGLETRGAALANSISYWINALIL+
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILM 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHN  YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNFSYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLV 360
           ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPA AKLAA +VMTIAV EGLL+
Sbjct: 301 ETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAGAKLAACLVMTIAVFEGLLL 360

Query: 361 ATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAY 420
            TVL+LIRNVWGYAYSNE EVVKYVAD+VPIIAVS+F DGLQCVLSGI RGCGWQKIGAY
Sbjct: 361 GTVLILIRNVWGYAYSNEQEVVKYVADIVPIIAVSTFFDGLQCVLSGIVRGCGWQKIGAY 420

Query: 421 VNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIAT 480
           VNLGSYYLVGIPSAVLLAFV+H+GGKGLWLGII ALAVQTLSLAIITIRTNWD+EAKIA 
Sbjct: 421 VNLGSYYLVGIPSAVLLAFVLHIGGKGLWLGIISALAVQTLSLAIITIRTNWDEEAKIAA 480

Query: 481 ERVYNAVIPTNAIS 495
           ERVY +VIPTN I+
Sbjct: 481 ERVYESVIPTNVIA 494

BLAST of Lag0033972 vs. ExPASy TrEMBL
Match: A0A0A0LV09 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_1G024880 PE=3 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.4e-244
Identity = 447/490 (91.22%), Postives = 466/490 (95.10%), Query Frame = 0

Query: 5   QNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQ 64
           QNSS NS L+H SEDEVN KDER  D +N RRKLIAEEVKKQLWLAGPLI VSLLQYCLQ
Sbjct: 7   QNSSFNSPLIHKSEDEVNSKDERQIDYENIRRKLIAEEVKKQLWLAGPLILVSLLQYCLQ 66

Query: 65  MISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHM 124
           MISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIH+
Sbjct: 67  MISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHL 126

Query: 125 QRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNR 184
           QRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQCL R
Sbjct: 127 QRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQCLYR 186

Query: 185 FLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVK 244
           FLQTQNVVFPMM+SSGI+AL HI LCW+MVFK GL TRGAA+ANSISYWINALILILYVK
Sbjct: 187 FLQTQNVVFPMMMSSGISALSHITLCWLMVFKVGLGTRGAAVANSISYWINALILILYVK 246

Query: 245 FSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSV 304
           FSSS SK+WTGFS  AFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSV
Sbjct: 247 FSSSSSKTWTGFSRLAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSV 306

Query: 305 LSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVL 364
           LSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLA  +VMTIAVIEGLL+ATVL
Sbjct: 307 LSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAGYVVMTIAVIEGLLLATVL 366

Query: 365 VLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLG 424
           +L+RNVWGYAYSNE EVV+YVA+VVP+IAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLG
Sbjct: 367 ILVRNVWGYAYSNEQEVVEYVANVVPLIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLG 426

Query: 425 SYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIATERVY 484
           SYYLVGIPSAVLLAFV HVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAK ATERVY
Sbjct: 427 SYYLVGIPSAVLLAFVFHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKKATERVY 486

Query: 485 NAVIPTNAIS 495
           +AVIP NAIS
Sbjct: 487 DAVIPVNAIS 496

BLAST of Lag0033972 vs. ExPASy TrEMBL
Match: A0A6J1L194 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498176 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.1e-244
Identity = 450/525 (85.71%), Postives = 470/525 (89.52%), Query Frame = 0

Query: 1   MARDQNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQ 60
           M RDQ S L S L+HISEDEVNLKD  L DD NF RKLIAEEVKKQLWLAGPLISVS LQ
Sbjct: 1   MERDQESWLKSPLIHISEDEVNLKDGGLVDDNNFSRKLIAEEVKKQLWLAGPLISVSFLQ 60

Query: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHML 120
           YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVL+GMASALDTFCGQSYGAKQYHML
Sbjct: 61  YCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLMGMASALDTFCGQSYGAKQYHML 120

Query: 121 GIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180
           GI+MQRAMFVL L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQ
Sbjct: 121 GIYMQRAMFVLSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQ 180

Query: 181 CLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILI 240
           CLNRFLQTQN+VFPMM+SSGIAALLHI LCWVM+FKAGLETRGAALANSISYWINALILI
Sbjct: 181 CLNRFLQTQNIVFPMMMSSGIAALLHIALCWVMIFKAGLETRGAALANSISYWINALILI 240

Query: 241 LYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300
           LYVKFSSSCS+SWTGFSAQAFHNI YFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL
Sbjct: 241 LYVKFSSSCSESWTGFSAQAFHNISYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKL 300

Query: 301 ETSVLSI-------------------------------SLNTDLTVWMIPMGLSAAASTR 360
           ETSVLSI                               SLNTDLTVWMIPMGLSAAASTR
Sbjct: 301 ETSVLSIRYKQRHQFFRSNVIFFCYIQPPLACPTCFFTSLNTDLTVWMIPMGLSAAASTR 360

Query: 361 VSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVLVLIRNVWGYAYSNEPEVVKYVADVV 420
           VSNELGAGRPA AKLAA +VMTIAVIEGLL+ TVL+LIRNVWGYAYSNE EVVKYVAD+V
Sbjct: 361 VSNELGAGRPAGAKLAACLVMTIAVIEGLLLGTVLILIRNVWGYAYSNEQEVVKYVADIV 420

Query: 421 PIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVMHVGGKGLW 480
           PIIAVS+F DGLQCVLSGI RGCGWQKIGAYVNLGSYYLVGIPSAVLLAFV+H+ GKGLW
Sbjct: 421 PIIAVSTFFDGLQCVLSGIVRGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVLHIDGKGLW 480

Query: 481 LGIICALAVQTLSLAIITIRTNWDQEAKIATERVYNAVIPTNAIS 495
           LGIICALAVQTLSLAIITIRTNWD+EAKIA ERVY +VIPTN I+
Sbjct: 481 LGIICALAVQTLSLAIITIRTNWDEEAKIAAERVYESVIPTNVIA 525

BLAST of Lag0033972 vs. ExPASy TrEMBL
Match: A0A1S3BX38 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103494162 PE=3 SV=1)

HSP 1 Score: 848.6 bits (2191), Expect = 1.3e-242
Identity = 443/490 (90.41%), Postives = 466/490 (95.10%), Query Frame = 0

Query: 5   QNSSLNSSLLHISEDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQ 64
           +NSSLNS L+H SED VN KDE   + +N RRKLIAEEVKKQLWLAGPLISVSLLQYCLQ
Sbjct: 7   RNSSLNSPLIHKSEDGVNSKDESQINYENIRRKLIAEEVKKQLWLAGPLISVSLLQYCLQ 66

Query: 65  MISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHM 124
           MISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIH+
Sbjct: 67  MISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHV 126

Query: 125 QRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNR 184
           QRAMF+L L+SIPLAVIWANTGEIL+L+GQDAQISAEAGKYAKCLIPCLFAHGLVQCLNR
Sbjct: 127 QRAMFILSLVSIPLAVIWANTGEILKLLGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNR 186

Query: 185 FLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVK 244
           FL TQNVVFPMM+SSGI+AL HI LCW+MVFK GL TRGAA+ANSISYWINALILILYVK
Sbjct: 187 FLTTQNVVFPMMMSSGISALSHIPLCWLMVFKIGLGTRGAAVANSISYWINALILILYVK 246

Query: 245 FSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSV 304
           FSSS SK+WTGFS  AFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSV
Sbjct: 247 FSSSSSKTWTGFSGLAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSV 306

Query: 305 LSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVL 364
           LSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLA  +VMTIAVIEGLL+AT+L
Sbjct: 307 LSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAGCVVMTIAVIEGLLLATIL 366

Query: 365 VLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLG 424
           +LIRNVWGYAYSNE EVV+YVA+VVP+IAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLG
Sbjct: 367 ILIRNVWGYAYSNEQEVVEYVANVVPLIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLG 426

Query: 425 SYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIATERVY 484
           SYYLVGIPSAVLLAFV+HVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAK ATERVY
Sbjct: 427 SYYLVGIPSAVLLAFVLHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKKATERVY 486

Query: 485 NAVIPTNAIS 495
           + VIP NAIS
Sbjct: 487 DTVIPVNAIS 496

BLAST of Lag0033972 vs. TAIR 10
Match: AT5G52450.1 (MATE efflux family protein )

HSP 1 Score: 610.1 bits (1572), Expect = 1.5e-174
Identity = 303/445 (68.09%), Postives = 367/445 (82.47%), Query Frame = 0

Query: 39  IAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLL 98
           + EEVKKQLWL+GPLI+VSLLQ+CLQ+IS+MFVGHLG LPLS AS+A SF SVTGF+ L+
Sbjct: 23  VKEEVKKQLWLSGPLIAVSLLQFCLQVISVMFVGHLGSLPLSAASIATSFASVTGFSFLM 82

Query: 99  GMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQI 158
           G ASALDT CGQ+YGAK+Y MLGI MQRAMFVL L SIPL++IWANT  +L   GQ+  I
Sbjct: 83  GTASALDTLCGQAYGAKKYGMLGIQMQRAMFVLTLASIPLSIIWANTEHLLVFFGQNKSI 142

Query: 159 SAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAG 218
           +  AG YAK +IP +FA+GL+QC NRFLQ QN VFP++  SG+   LH+LLCWV+VFK+G
Sbjct: 143 ATLAGSYAKFMIPSIFAYGLLQCFNRFLQAQNNVFPVVFCSGVTTSLHVLLCWVLVFKSG 202

Query: 219 LETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMV 278
           L  +GAALANSISYW+N ++L  YVKFS SCS +WTGFS +A  +I  FL+LA+PS +MV
Sbjct: 203 LGFQGAALANSISYWLNVVLLFCYVKFSPSCSLTWTGFSKEALRDILPFLRLAVPSALMV 262

Query: 279 CLELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGR 338
           CLE+WSFE++VLLSGLLPNP LETSVLSI LNT  T+WMIP GLS AASTR+SNELGAG 
Sbjct: 263 CLEMWSFELLVLLSGLLPNPVLETSVLSICLNTSGTMWMIPFGLSGAASTRISNELGAGN 322

Query: 339 PAAAKLAASIVMTIAVIEGLLVATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFL 398
           P  AKLA  +V+ IAV E +++ +VL+LIRN+WG AYS+E EVV YVA ++PI+A+ +FL
Sbjct: 323 PKVAKLAVRVVICIAVAESIVIGSVLILIRNIWGLAYSSELEVVSYVASMMPILALGNFL 382

Query: 399 DGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAV 458
           D LQCVLSG+ARGCGWQKIGA +NLGSYYLVG+PS +LLAF  HVGG+GLWLGIICAL V
Sbjct: 383 DSLQCVLSGVARGCGWQKIGAIINLGSYYLVGVPSGLLLAFHFHVGGRGLWLGIICALVV 442

Query: 459 QTLSLAIITIRTNWDQEAKIATERV 484
           Q   L ++TI TNWD+EAK AT R+
Sbjct: 443 QVFGLGLVTIFTNWDEEAKKATNRI 467

BLAST of Lag0033972 vs. TAIR 10
Match: AT1G73700.1 (MATE efflux family protein )

HSP 1 Score: 587.4 bits (1513), Expect = 1.1e-167
Identity = 299/456 (65.57%), Postives = 361/456 (79.17%), Query Frame = 0

Query: 28  LADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVS 87
           L  +K+     + EEVKKQLWL+ PLI VSLLQY LQ+IS+MFVGHLG LPLS AS+A S
Sbjct: 10  LITEKDTTMIRVKEEVKKQLWLSAPLIGVSLLQYSLQVISVMFVGHLGSLPLSAASIATS 69

Query: 88  FTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGE 147
           F SVTGFT LLG ASAL+T CGQ+YGAK Y  LGI MQRAMFVLL+LS+PL++IWANT +
Sbjct: 70  FASVTGFTFLLGTASALETLCGQAYGAKLYGKLGIQMQRAMFVLLILSVPLSIIWANTEQ 129

Query: 148 ILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAALLHI 207
           IL LV QD  I++ AG YAK +IP LFA+GL+QC+NRFLQ QN VFP+ V SGI   LH+
Sbjct: 130 ILVLVHQDKSIASVAGSYAKYMIPSLFAYGLLQCINRFLQAQNNVFPVFVCSGITTCLHL 189

Query: 208 LLCWVMVFKAGLETRGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYF 267
           LLCW+ V K GL  RGAALA S+SYW N ++L  YVKFS SCS SWTGFS +AF  +  F
Sbjct: 190 LLCWLFVLKTGLGYRGAALAISVSYWFNVILLSCYVKFSPSCSHSWTGFSKEAFQELYDF 249

Query: 268 LKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAAS 327
            K+A PS VMVCLELWSFE++VL SGLLPNP LETSVLSI LNT LT+W I +GL  AAS
Sbjct: 250 SKIAFPSAVMVCLELWSFELLVLASGLLPNPVLETSVLSICLNTSLTIWQISVGLGGAAS 309

Query: 328 TRVSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVLVLIRNVWGYAYSNEPEVVKYVAD 387
            RVSNELGAG P  AKLA  +++ IAV EG++V TVL+ IR + G+A+S++P+++ Y A 
Sbjct: 310 IRVSNELGAGNPQVAKLAVYVIVGIAVAEGIVVVTVLLSIRKILGHAFSSDPKIIAYAAS 369

Query: 388 VVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVMHVGGKG 447
           ++PI+A  +FLDGLQCVLSG+ARGCGWQKIGA VNLGSYYLVG+P  +LL F  H+GG+G
Sbjct: 370 MIPIVACGNFLDGLQCVLSGVARGCGWQKIGACVNLGSYYLVGVPLGLLLGFHFHIGGRG 429

Query: 448 LWLGIICALAVQTLSLAIITIRTNWDQEAKIATERV 484
           LWLGI+ AL+VQ L L+++TI TNWD+EAK AT RV
Sbjct: 430 LWLGIVTALSVQVLCLSLVTIFTNWDKEAKKATNRV 465

BLAST of Lag0033972 vs. TAIR 10
Match: AT2G34360.1 (MATE efflux family protein )

HSP 1 Score: 560.8 bits (1444), Expect = 1.1e-159
Identity = 285/466 (61.16%), Postives = 359/466 (77.04%), Query Frame = 0

Query: 18  EDEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGEL 77
           E+  ++    L  +K  R + + EEV+KQL L+GPLI+VSLLQ+CLQ+IS+MFVGHLG L
Sbjct: 3   EEREDMLSWPLIGEKEKRSRFVKEEVEKQLLLSGPLIAVSLLQFCLQIISVMFVGHLGSL 62

Query: 78  PLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIP 137
           PLS AS+A SF SVTGFT L+G ASA+DT CGQSYGAK Y MLGI MQRAM VL LLS+P
Sbjct: 63  PLSAASIATSFASVTGFTFLMGTASAMDTVCGQSYGAKMYGMLGIQMQRAMLVLTLLSVP 122

Query: 138 LAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMV 197
           L+++WANT   L   GQD  I+  +G YA+ +IP +FA+GL+QCLNRFLQ QN V P+++
Sbjct: 123 LSIVWANTEHFLVFFGQDKSIAHLSGSYARFMIPSIFAYGLLQCLNRFLQAQNNVIPVVI 182

Query: 198 SSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVKFSSSCSKSWTGFS 257
            SG+   LH+++CWV+V K+GL  RGAA+AN+ISYW+N ++L  YVKFS SCS +WTGFS
Sbjct: 183 CSGVTTSLHVIICWVLVLKSGLGFRGAAVANAISYWLNVILLSCYVKFSPSCSLTWTGFS 242

Query: 258 AQAFHNIPYFLKLAIPSTVMVC-LELWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVW 317
            +A  +I  F+KL IPS  MVC LE+WSFE++VL SGLLPNP LETS          TVW
Sbjct: 243 KEARRDIIPFMKLVIPSAFMVCSLEMWSFELLVLSSGLLPNPVLETS-------CPRTVW 302

Query: 318 MIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVLVLIRNVWGYAYS 377
           MIP GLS AASTRVSNELG+G P  AKLA  +V++ +++E +LV TVL+LIR +WG+AYS
Sbjct: 303 MIPFGLSGAASTRVSNELGSGNPKGAKLAVRVVLSFSIVESILVGTVLILIRKIWGFAYS 362

Query: 378 NEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVL 437
           ++PEVV +VA ++PI+A+   LD  Q VLSG+ARGCGWQKIGA+VNLGSYYLVG+P  +L
Sbjct: 363 SDPEVVSHVASMLPILALGHSLDSFQTVLSGVARGCGWQKIGAFVNLGSYYLVGVPFGLL 422

Query: 438 LAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIATER 483
           L F  HVGG+GLWLGIICAL VQ + L++IT  TNWD+E K AT R
Sbjct: 423 LGFHFHVGGRGLWLGIICALIVQGVCLSLITFFTNWDEEVKKATSR 461

BLAST of Lag0033972 vs. TAIR 10
Match: AT1G71140.1 (MATE efflux family protein )

HSP 1 Score: 439.9 bits (1130), Expect = 2.7e-123
Identity = 239/477 (50.10%), Postives = 322/477 (67.51%), Query Frame = 0

Query: 8   SLNSSLLHISE-DEVNLKDERLADDKNFRRKLIAEEVKKQLWLAGPLISVSLLQYCLQMI 67
           S    LL +S+ +EVN KD  L             E KK  ++AGP+I+V+   Y LQ+I
Sbjct: 3   SAEKGLLVVSDREEVNKKDGFL------------RETKKLSYIAGPMIAVNSSMYVLQVI 62

Query: 68  SIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMASALDTFCGQSYGAKQYHMLGIHMQR 127
           SIM VGHLGEL LS  ++AVSF SVTGF+V+ G+ASAL+T CGQ+ GAKQY  LG+H   
Sbjct: 63  SIMMVGHLGELFLSSTAIAVSFCSVTGFSVVFGLASALETLCGQANGAKQYEKLGVHTYT 122

Query: 128 AMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAEAGKYAKCLIPCLFAHGLVQCLNRFL 187
            +  L L+ IPL+++W   G+IL L+GQDA ++ EAGK+A  LIP LF +  +Q L RF 
Sbjct: 123 GIVSLFLVCIPLSLLWTYIGDILSLIGQDAMVAQEAGKFATWLIPALFGYATLQPLVRFF 182

Query: 188 QTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLETRGAALANSISYWINALILILYVKFS 247
           Q Q+++ P+++SS  +  +HI+LCW +VFK GL + GAA+A  +SYW+N  +L LY+ FS
Sbjct: 183 QAQSLILPLVMSSVSSLCIHIVLCWSLVFKFGLGSLGAAIAIGVSYWLNVTVLGLYMTFS 242

Query: 248 SSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLELWSFEMVVLLSGLLPNPKLETSVLS 307
           SSCSKS    S   F  +  F +  IPS  M+CLE WSFE +VLLSG+LPNPKLE SVLS
Sbjct: 243 SSCSKSRATISMSLFEGMGEFFRFGIPSASMICLEWWSFEFLVLLSGILPNPKLEASVLS 302

Query: 308 ISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAAAKLAASIVMTIAVIEGLLVATVLVL 367
           + L+T  +++ IP  L AAASTRV+NELGAG P  A++A    M I  +E ++V  ++  
Sbjct: 303 VCLSTQSSLYQIPESLGAAASTRVANELGAGNPKQARMAVYTAMVITGVESIMVGAIVFG 362

Query: 368 IRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGLQCVLSGIARGCGWQKIGAYVNLGSY 427
            RNV+GY +S+E EVV YV  + P++++S   D L   LSG+ARG G Q IGAYVNL +Y
Sbjct: 363 ARNVFGYLFSSETEVVDYVKSMAPLLSLSVIFDALHAALSGVARGSGRQDIGAYVNLAAY 422

Query: 428 YLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTLSLAIITIRTNWDQEAKIATERV 484
           YL GIP+A+LLAF   + G+GLW+GI     VQ + L +I I TNW ++A+ A ERV
Sbjct: 423 YLFGIPTAILLAFGFKMRGRGLWIGITVGSCVQAVLLGLIVILTNWKKQARKARERV 467

BLAST of Lag0033972 vs. TAIR 10
Match: AT1G15170.1 (MATE efflux family protein )

HSP 1 Score: 421.4 bits (1082), Expect = 1.0e-117
Identity = 213/442 (48.19%), Postives = 316/442 (71.49%), Query Frame = 0

Query: 42  EVKKQLWLAGPLISVSLLQYCLQMISIMFVGHLGELPLSGASMAVSFTSVTGFTVLLGMA 101
           E+K+ ++ A P+ +V + Q+ LQ++S+M VGHLG L L+ AS+A SF +VTGF+ ++G++
Sbjct: 34  ELKRLIFFAAPMAAVVIAQFMLQIVSMMMVGHLGNLSLASASLASSFCNVTGFSFIIGLS 93

Query: 102 SALDTFCGQSYGAKQYHMLGIHMQRAMFVLLLLSIPLAVIWANTGEILQLVGQDAQISAE 161
            ALDT  GQ+YGAK Y  LG+    AMF L L+ +PL++IW N  ++L ++GQD  I+ E
Sbjct: 94  CALDTLSGQAYGAKLYRKLGVQTYTAMFCLALVCLPLSLIWFNMEKLLLILGQDPSIAHE 153

Query: 162 AGKYAKCLIPCLFAHGLVQCLNRFLQTQNVVFPMMVSSGIAALLHILLCWVMVFKAGLET 221
           AGKYA  LIP LFA+ ++Q L R+ Q Q+++ P++++S +   +H+ LCW +V+ +GL  
Sbjct: 154 AGKYATWLIPGLFAYAVLQPLTRYFQNQSLITPLLITSYVVFCIHVPLCWFLVYNSGLGN 213

Query: 222 RGAALANSISYWINALILILYVKFSSSCSKSWTGFSAQAFHNIPYFLKLAIPSTVMVCLE 281
            G ALA S+S W+ A+ L  ++ +SS+CS++    S + F  I  F K A+PS  M+CLE
Sbjct: 214 LGGALAISLSNWLYAIFLGSFMYYSSACSETRAPLSMEIFDGIGEFFKYALPSAAMICLE 273

Query: 282 LWSFEMVVLLSGLLPNPKLETSVLSISLNTDLTVWMIPMGLSAAASTRVSNELGAGRPAA 341
            WS+E+++LLSGLLPNP+LETSVLS+ L T  T++ IP+ ++AAASTR+SNELGAG   A
Sbjct: 274 WWSYELIILLSGLLPNPQLETSVLSVCLQTISTMYSIPLAIAAAASTRISNELGAGNSRA 333

Query: 342 AKLAASIVMTIAVIEGLLVATVLVLIRNVWGYAYSNEPEVVKYVADVVPIIAVSSFLDGL 401
           A +     M++AVI+ L+V+  L++ RN++G+ +S++ E + YVA + P++++S  LD L
Sbjct: 334 AHIVVYAAMSLAVIDALIVSMSLLIGRNLFGHIFSSDKETIDYVAKMAPLVSISLMLDAL 393

Query: 402 QCVLSGIARGCGWQKIGAYVNLGSYYLVGIPSAVLLAFVMHVGGKGLWLGIICALAVQTL 461
           Q VLSGIARGCGWQ IGAY+NLG++YL GIP A  LAF +H+ G GLW+GI     +QTL
Sbjct: 394 QGVLSGIARGCGWQHIGAYINLGAFYLWGIPIAASLAFWIHLKGVGLWIGIQAGAVLQTL 453

Query: 462 SLAIITIRTNWDQEAKIATERV 484
            LA++T  TNW+ +A  A  R+
Sbjct: 454 LLALVTGCTNWESQADKARNRM 475

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023531831.18.8e-24991.09protein DETOXIFICATION 16-like [Cucurbita pepo subsp. pepo][more]
XP_023005095.11.1e-24891.09protein DETOXIFICATION 16-like isoform X2 [Cucurbita maxima][more]
XP_022929082.19.7e-24890.69protein DETOXIFICATION 16-like isoform X1 [Cucurbita moschata][more]
KAG6587669.11.4e-24690.49Protein DETOXIFICATION 16, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_004137332.25.0e-24491.22protein DETOXIFICATION 16 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9FHB62.1e-17368.09Protein DETOXIFICATION 16 OS=Arabidopsis thaliana OX=3702 GN=DTX16 PE=2 SV=1[more]
Q9C9U11.5e-16665.57Protein DETOXIFICATION 17 OS=Arabidopsis thaliana OX=3702 GN=DTX17 PE=2 SV=1[more]
F4IHU91.5e-15861.16Protein DETOXIFICATION 15 OS=Arabidopsis thaliana OX=3702 GN=DTX15 PE=3 SV=1[more]
Q9C9943.8e-12250.10Protein DETOXIFICATION 14 OS=Arabidopsis thaliana OX=3702 GN=DTX14 PE=1 SV=1[more]
Q8L7311.4e-11648.19Protein DETOXIFICATION 12 OS=Arabidopsis thaliana OX=3702 GN=DTX12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KWH95.6e-24991.09Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498176 PE=3 SV=1[more]
A0A6J1ELR84.7e-24890.69Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111435773 PE=3 SV=1[more]
A0A0A0LV092.4e-24491.22Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_1G024880 PE=3 SV=1[more]
A0A6J1L1944.1e-24485.71Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111498176 PE=3 SV=1[more]
A0A1S3BX381.3e-24290.41Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103494162 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G52450.11.5e-17468.09MATE efflux family protein [more]
AT1G73700.11.1e-16765.57MATE efflux family protein [more]
AT2G34360.11.1e-15961.16MATE efflux family protein [more]
AT1G71140.12.7e-12350.10MATE efflux family protein [more]
AT1G15170.11.0e-11748.19MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 273..435
e-value: 1.3E-29
score: 102.9
coord: 52..212
e-value: 2.9E-38
score: 131.0
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 52..449
e-value: 9.3E-94
score: 312.5
NoneNo IPR availablePANTHERPTHR11206:SF196PROTEIN DETOXIFICATIONcoord: 25..482
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 25..482
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 42..477
e-value: 0.0
score: 537.926

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033972.1Lag0033972.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity