Lag0033711 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0033711
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionHydroxymethylbilane hydrolyase [cyclizing]
Locationchr3: 1292898 .. 1293830 (-)
RNA-Seq ExpressionLag0033711
SyntenyLag0033711
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCACCGGCGCCGCCCATCTCAGCCCAATCCACCAAAATCCACTCACTTCATCTCCTTCTCCCGCCATTTCCACCCACTCCCCTCCGCGTACGGCGGTGTTCACGACGCCTCAGAACTACGCCGGCAGCCTCTCCCACCTCCTCTCTCTCAAAGGCTTCGAACCCCTCTGGTGCCCCACTCTCACCGTCCACCACACTCCCCTCGCCATCAAATCCCATCTCCTTCCTCCAAATCTCCATTCCTTCTCCGCCGTCGCCTTCACTTCCCGCTCCGGCATCACAGCCGTTCTCGACGCCGCTACTGAAGTCGGCAAGCCCTTGCTACCGTCGCACGGCGACACTTTTCTAATCGCAGCCCTAGGTAAGGACTCCGAGCTTCTCGACCATGAATTTCTTTCCAAAATTTGCCCTAACGCGAGTCGAATTAAAATCGTGGTACCTGAACTTGCAACTCCGAGTGGTCTAGTGGAGGCTCTTGGAGCTGGAAACCACCGTAGGGTTCTGTGTCCGGTTCCTCGCGTCATAGGGCTGAACGAGCCTCCGGTGGTTCCGAACTTCCTCCGTGACCTGGAGGCGAACGGGTGGGTTCCGGCTCGTGCCGATGCGTACGAGACCCGATGGGCCGGACCCGAGTGCGCGAGGAAGCTTGTGGAGAGAAGGCAGGATGAGAAATTGGATGCCATTGTGTTTACGAGTACTGGGGAAGTGGAGGGGCTGCTCAAGAGCTTGAGGCATCTGGGATTAGAGTGGGAGATGATGAGAAAGAGGTGGCCGGAAATGGTGGTGGCCGCGCACGGGCCGGTGACGGCGGCGGGAGCGGAGAGGCTCGGCGTCAAGGTTGACTTGGTGAGTTCTAAATTCGATAGCTTCAATGGTGTGGTTGATGCTCTTCATTCGAGATGGCAGAGCTTAGAACAGAATCCTGTGTAA

mRNA sequence

ATGAGCACCGGCGCCGCCCATCTCAGCCCAATCCACCAAAATCCACTCACTTCATCTCCTTCTCCCGCCATTTCCACCCACTCCCCTCCGCGTACGGCGGTGTTCACGACGCCTCAGAACTACGCCGGCAGCCTCTCCCACCTCCTCTCTCTCAAAGGCTTCGAACCCCTCTGGTGCCCCACTCTCACCGTCCACCACACTCCCCTCGCCATCAAATCCCATCTCCTTCCTCCAAATCTCCATTCCTTCTCCGCCGTCGCCTTCACTTCCCGCTCCGGCATCACAGCCGTTCTCGACGCCGCTACTGAAGTCGGCAAGCCCTTGCTACCGTCGCACGGCGACACTTTTCTAATCGCAGCCCTAGGTAAGGACTCCGAGCTTCTCGACCATGAATTTCTTTCCAAAATTTGCCCTAACGCGAGTCGAATTAAAATCGTGGTACCTGAACTTGCAACTCCGAGTGGTCTAGTGGAGGCTCTTGGAGCTGGAAACCACCGTAGGGTTCTGTGTCCGGTTCCTCGCGTCATAGGGCTGAACGAGCCTCCGGTGGTTCCGAACTTCCTCCGTGACCTGGAGGCGAACGGGTGGGTTCCGGCTCGTGCCGATGCGTACGAGACCCGATGGGCCGGACCCGAGTGCGCGAGGAAGCTTGTGGAGAGAAGGCAGGATGAGAAATTGGATGCCATTGTGTTTACGAGTACTGGGGAAGTGGAGGGGCTGCTCAAGAGCTTGAGGCATCTGGGATTAGAGTGGGAGATGATGAGAAAGAGGTGGCCGGAAATGGTGGTGGCCGCGCACGGGCCGGTGACGGCGGCGGGAGCGGAGAGGCTCGGCGTCAAGGTTGACTTGGTGAGTTCTAAATTCGATAGCTTCAATGGTGTGGTTGATGCTCTTCATTCGAGATGGCAGAGCTTAGAACAGAATCCTGTGTAA

Coding sequence (CDS)

ATGAGCACCGGCGCCGCCCATCTCAGCCCAATCCACCAAAATCCACTCACTTCATCTCCTTCTCCCGCCATTTCCACCCACTCCCCTCCGCGTACGGCGGTGTTCACGACGCCTCAGAACTACGCCGGCAGCCTCTCCCACCTCCTCTCTCTCAAAGGCTTCGAACCCCTCTGGTGCCCCACTCTCACCGTCCACCACACTCCCCTCGCCATCAAATCCCATCTCCTTCCTCCAAATCTCCATTCCTTCTCCGCCGTCGCCTTCACTTCCCGCTCCGGCATCACAGCCGTTCTCGACGCCGCTACTGAAGTCGGCAAGCCCTTGCTACCGTCGCACGGCGACACTTTTCTAATCGCAGCCCTAGGTAAGGACTCCGAGCTTCTCGACCATGAATTTCTTTCCAAAATTTGCCCTAACGCGAGTCGAATTAAAATCGTGGTACCTGAACTTGCAACTCCGAGTGGTCTAGTGGAGGCTCTTGGAGCTGGAAACCACCGTAGGGTTCTGTGTCCGGTTCCTCGCGTCATAGGGCTGAACGAGCCTCCGGTGGTTCCGAACTTCCTCCGTGACCTGGAGGCGAACGGGTGGGTTCCGGCTCGTGCCGATGCGTACGAGACCCGATGGGCCGGACCCGAGTGCGCGAGGAAGCTTGTGGAGAGAAGGCAGGATGAGAAATTGGATGCCATTGTGTTTACGAGTACTGGGGAAGTGGAGGGGCTGCTCAAGAGCTTGAGGCATCTGGGATTAGAGTGGGAGATGATGAGAAAGAGGTGGCCGGAAATGGTGGTGGCCGCGCACGGGCCGGTGACGGCGGCGGGAGCGGAGAGGCTCGGCGTCAAGGTTGACTTGGTGAGTTCTAAATTCGATAGCTTCAATGGTGTGGTTGATGCTCTTCATTCGAGATGGCAGAGCTTAGAACAGAATCCTGTGTAA

Protein sequence

MSTGAAHLSPIHQNPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWCPTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIAALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLNEPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEGLLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALHSRWQSLEQNPV
Homology
BLAST of Lag0033711 vs. NCBI nr
Match: XP_008444040.1 (PREDICTED: uncharacterized protein LOC103487492 [Cucumis melo] >KAA0050031.1 Tetrapyrrole biosynthesis, uroporphyrinogen III synthase [Cucumis melo var. makuwa])

HSP 1 Score: 552.0 bits (1421), Expect = 3.3e-153
Identity = 269/307 (87.62%), Postives = 286/307 (93.16%), Query Frame = 0

Query: 1   MSTGAAHLS-PIHQNPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAH + P H +PLTSSPSPAI THS PRT  FTTPQNYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIA 120
           PTLTV  TPLAIKSHLLPP LHSFSAVAFTSRSGITA+LDAATE+G+PLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLN 180
           ALGKDSELLDHEFL+ ICPN SRI++VVPE+ATP+GLVEALG GNHRRVLCPVPRV+GLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEA GWVP R DAYETRWAGP+CARKLVER +DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300
           LLKSL HLGLEW+MM+KRWPEMVVAAHGPVTAAGAERLGVKVDLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 307
            RWQSL+
Sbjct: 301 WRWQSLD 307

BLAST of Lag0033711 vs. NCBI nr
Match: TYK03635.1 (Tetrapyrrole biosynthesis, uroporphyrinogen III synthase [Cucumis melo var. makuwa])

HSP 1 Score: 550.4 bits (1417), Expect = 9.7e-153
Identity = 268/307 (87.30%), Postives = 286/307 (93.16%), Query Frame = 0

Query: 1   MSTGAAHLS-PIHQNPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAH + P H +PLTSSPSPAI THS PRT  FTTPQNYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIA 120
           PTLTV  TPLAIKSHLLPP LHSFSAVAFTSRSGITA+LDAATE+G+PLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLN 180
           ALGKDSELLDHEFL+ ICPN SRI++VVPE+ATP+GLVEALG GNHRRVLCPVPRV+GLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEA GWVP R DAYETRWAGP+CARKLVER +DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300
           LLKSL HLGLEW++M+KRWPEMVVAAHGPVTAAGAERLGVKVDLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDIMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 307
            RWQSL+
Sbjct: 301 WRWQSLD 307

BLAST of Lag0033711 vs. NCBI nr
Match: KAG6587847.1 (hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035661.1 hypothetical protein SDJN02_02459, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 542.7 bits (1397), Expect = 2.0e-150
Identity = 265/310 (85.48%), Postives = 285/310 (91.94%), Query Frame = 0

Query: 1   MSTGAAHLSPIHQ-NPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAH SPIH  NPL SSPSPAI T+SP RT  FTTPQNYAGSLS+LLSLKGF+PLWC
Sbjct: 1   MSTGAAHPSPIHHLNPLASSPSPAIPTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIA 120
           PT+TVH TPLAIKSHLLPPNLH +SAVAFTSRSGITA+LDAATE+ +PLL   GDTFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLN 180
           ALGKDSELLDH  LSK CPNASRI++VVP++A+PSGLVEALG GNHRRVLCPVPRV+GLN
Sbjct: 121 ALGKDSELLDHGVLSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A+GWVP R DAYETRW GPECARKL ER +DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300
           LLKSLR LGL+WEMMRK+WPEMV+AAHGPVTAAGAERLGVK+DLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 310
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

BLAST of Lag0033711 vs. NCBI nr
Match: XP_011648476.1 (uncharacterized protein LOC105434481 [Cucumis sativus] >KGN65221.1 hypothetical protein Csa_019699 [Cucumis sativus])

HSP 1 Score: 542.3 bits (1396), Expect = 2.6e-150
Identity = 266/307 (86.64%), Postives = 283/307 (92.18%), Query Frame = 0

Query: 1   MSTGAAHLS-PIHQNPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAH + P H N LTSSPSPAI TH  PRTA FTTP NYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHLNSLTSSPSPAIPTHFSPRTAAFTTPPNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIA 120
           PTLTV  TPLAIKSHLLPP LHSFSAVAFTSRSGITA+LDAATE+G+PLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLN 180
           ALGKDSELLDHEFL+ IC N SRI++VVPE+ATP+GLVEALG GNHR VLCPVPRV+GLN
Sbjct: 121 ALGKDSELLDHEFLTTICHNTSRIRVVVPEIATPTGLVEALGVGNHRSVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEA GWVP R DAYETRWAGP+CARKLVER +DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300
           LLKSL HLGLEW++M++RWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLAHLGLEWDVMKRRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLE 307
            RWQ+LE
Sbjct: 301 WRWQNLE 307

BLAST of Lag0033711 vs. NCBI nr
Match: XP_022926996.1 (uncharacterized protein LOC111433956 [Cucurbita moschata])

HSP 1 Score: 542.3 bits (1396), Expect = 2.6e-150
Identity = 265/310 (85.48%), Postives = 285/310 (91.94%), Query Frame = 0

Query: 1   MSTGAAHLSPI-HQNPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAH SPI H NPL SSPSPAIST+SP RT  FTTPQNYAGSLS+LLSLKGF+PLWC
Sbjct: 1   MSTGAAHPSPIRHLNPLASSPSPAISTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIA 120
           PT+TVH TPLAIKSHLLPPNLH +SAVAFTSRSGITA+LDAATE+ +PLL   GDTFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLN 180
           ALGKDSELLDH   SK CPNASRI++VVP++A+PSGLVEALG GNHRRVLCPVPRV+GLN
Sbjct: 121 ALGKDSELLDHGVFSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A+GWVP R DAYETRW GPECARKL ER +DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300
           LLKSLR LGL+WEMMRK+WPEMV+AAHGPVTAAGAERLGVK+DLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 310
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

BLAST of Lag0033711 vs. ExPASy TrEMBL
Match: A0A5A7U8V3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00050 PE=3 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.6e-153
Identity = 269/307 (87.62%), Postives = 286/307 (93.16%), Query Frame = 0

Query: 1   MSTGAAHLS-PIHQNPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAH + P H +PLTSSPSPAI THS PRT  FTTPQNYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIA 120
           PTLTV  TPLAIKSHLLPP LHSFSAVAFTSRSGITA+LDAATE+G+PLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLN 180
           ALGKDSELLDHEFL+ ICPN SRI++VVPE+ATP+GLVEALG GNHRRVLCPVPRV+GLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEA GWVP R DAYETRWAGP+CARKLVER +DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300
           LLKSL HLGLEW+MM+KRWPEMVVAAHGPVTAAGAERLGVKVDLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 307
            RWQSL+
Sbjct: 301 WRWQSLD 307

BLAST of Lag0033711 vs. ExPASy TrEMBL
Match: A0A1S3BA78 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo OX=3656 GN=LOC103487492 PE=3 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.6e-153
Identity = 269/307 (87.62%), Postives = 286/307 (93.16%), Query Frame = 0

Query: 1   MSTGAAHLS-PIHQNPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAH + P H +PLTSSPSPAI THS PRT  FTTPQNYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIA 120
           PTLTV  TPLAIKSHLLPP LHSFSAVAFTSRSGITA+LDAATE+G+PLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLN 180
           ALGKDSELLDHEFL+ ICPN SRI++VVPE+ATP+GLVEALG GNHRRVLCPVPRV+GLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEA GWVP R DAYETRWAGP+CARKLVER +DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300
           LLKSL HLGLEW+MM+KRWPEMVVAAHGPVTAAGAERLGVKVDLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 307
            RWQSL+
Sbjct: 301 WRWQSLD 307

BLAST of Lag0033711 vs. ExPASy TrEMBL
Match: A0A5D3BX13 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00630 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 4.7e-153
Identity = 268/307 (87.30%), Postives = 286/307 (93.16%), Query Frame = 0

Query: 1   MSTGAAHLS-PIHQNPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAH + P H +PLTSSPSPAI THS PRT  FTTPQNYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHISPLTSSPSPAIPTHSSPRTVAFTTPQNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIA 120
           PTLTV  TPLAIKSHLLPP LHSFSAVAFTSRSGITA+LDAATE+G+PLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLN 180
           ALGKDSELLDHEFL+ ICPN SRI++VVPE+ATP+GLVEALG GNHRRVLCPVPRV+GLN
Sbjct: 121 ALGKDSELLDHEFLTTICPNTSRIRVVVPEIATPTGLVEALGVGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEA GWVP R DAYETRWAGP+CARKLVER +DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300
           LLKSL HLGLEW++M+KRWPEMVVAAHGPVTAAGAERLGVKVDLVS KFDSFNGVVD+LH
Sbjct: 241 LLKSLEHLGLEWDIMKKRWPEMVVAAHGPVTAAGAERLGVKVDLVSPKFDSFNGVVDSLH 300

Query: 301 SRWQSLE 307
            RWQSL+
Sbjct: 301 WRWQSLD 307

BLAST of Lag0033711 vs. ExPASy TrEMBL
Match: A0A0A0LZ08 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis sativus OX=3659 GN=Csa_1G266180 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.3e-150
Identity = 266/307 (86.64%), Postives = 283/307 (92.18%), Query Frame = 0

Query: 1   MSTGAAHLS-PIHQNPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAH + P H N LTSSPSPAI TH  PRTA FTTP NYAGSLSHLLSLKGFEPLWC
Sbjct: 1   MSTGAAHPNGPFHLNSLTSSPSPAIPTHFSPRTAAFTTPPNYAGSLSHLLSLKGFEPLWC 60

Query: 61  PTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIA 120
           PTLTV  TPLAIKSHLLPP LHSFSAVAFTSRSGITA+LDAATE+G+PLLPSHGDTFLIA
Sbjct: 61  PTLTVQPTPLAIKSHLLPPILHSFSAVAFTSRSGITALLDAATEIGEPLLPSHGDTFLIA 120

Query: 121 ALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLN 180
           ALGKDSELLDHEFL+ IC N SRI++VVPE+ATP+GLVEALG GNHR VLCPVPRV+GLN
Sbjct: 121 ALGKDSELLDHEFLTTICHNTSRIRVVVPEIATPTGLVEALGVGNHRSVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDLEA GWVP R DAYETRWAGP+CARKLVER +DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLEAKGWVPVRVDAYETRWAGPDCARKLVERGKDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300
           LLKSL HLGLEW++M++RWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLAHLGLEWDVMKRRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLE 307
            RWQ+LE
Sbjct: 301 WRWQNLE 307

BLAST of Lag0033711 vs. ExPASy TrEMBL
Match: A0A6J1EGG3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita moschata OX=3662 GN=LOC111433956 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.3e-150
Identity = 265/310 (85.48%), Postives = 285/310 (91.94%), Query Frame = 0

Query: 1   MSTGAAHLSPI-HQNPLTSSPSPAISTHSPPRTAVFTTPQNYAGSLSHLLSLKGFEPLWC 60
           MSTGAAH SPI H NPL SSPSPAIST+SP RT  FTTPQNYAGSLS+LLSLKGF+PLWC
Sbjct: 1   MSTGAAHPSPIRHLNPLASSPSPAISTNSPSRTVAFTTPQNYAGSLSNLLSLKGFDPLWC 60

Query: 61  PTLTVHHTPLAIKSHLLPPNLHSFSAVAFTSRSGITAVLDAATEVGKPLLPSHGDTFLIA 120
           PT+TVH TPLAIKSHLLPPNLH +SAVAFTSRSGITA+LDAATE+ +PLL   GDTFLIA
Sbjct: 61  PTVTVHPTPLAIKSHLLPPNLHFYSAVAFTSRSGITALLDAATEIDEPLLSPQGDTFLIA 120

Query: 121 ALGKDSELLDHEFLSKICPNASRIKIVVPELATPSGLVEALGAGNHRRVLCPVPRVIGLN 180
           ALGKDSELLDH   SK CPNASRI++VVP++A+PSGLVEALG GNHRRVLCPVPRV+GLN
Sbjct: 121 ALGKDSELLDHGVFSKFCPNASRIRVVVPKIASPSGLVEALGIGNHRRVLCPVPRVVGLN 180

Query: 181 EPPVVPNFLRDLEANGWVPARADAYETRWAGPECARKLVERRQDEKLDAIVFTSTGEVEG 240
           EPPVVPNFLRDL A+GWVP R DAYETRW GPECARKL ER +DEKLDAIVFTSTGEVEG
Sbjct: 181 EPPVVPNFLRDLAASGWVPVRVDAYETRWVGPECARKLAERGEDEKLDAIVFTSTGEVEG 240

Query: 241 LLKSLRHLGLEWEMMRKRWPEMVVAAHGPVTAAGAERLGVKVDLVSSKFDSFNGVVDALH 300
           LLKSLR LGL+WEMMRK+WPEMV+AAHGPVTAAGAERLGVK+DLVSSKFDSFNGVVDALH
Sbjct: 241 LLKSLRALGLKWEMMRKKWPEMVLAAHGPVTAAGAERLGVKIDLVSSKFDSFNGVVDALH 300

Query: 301 SRWQSLEQNP 310
           SRWQSLEQNP
Sbjct: 301 SRWQSLEQNP 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008444040.13.3e-15387.62PREDICTED: uncharacterized protein LOC103487492 [Cucumis melo] >KAA0050031.1 Tet... [more]
TYK03635.19.7e-15387.30Tetrapyrrole biosynthesis, uroporphyrinogen III synthase [Cucumis melo var. maku... [more]
KAG6587847.12.0e-15085.48hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_011648476.12.6e-15086.64uncharacterized protein LOC105434481 [Cucumis sativus] >KGN65221.1 hypothetical ... [more]
XP_022926996.12.6e-15085.48uncharacterized protein LOC111433956 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7U8V31.6e-15387.62Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3BA781.6e-15387.62Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo OX=3656 GN=LOC1034874... [more]
A0A5D3BX134.7e-15387.30Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469... [more]
A0A0A0LZ081.3e-15086.64Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis sativus OX=3659 GN=Csa_1G2... [more]
A0A6J1EGG31.3e-15085.48Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003754Tetrapyrrole biosynthesis, uroporphyrinogen III synthasePFAMPF02602HEM4coord: 45..292
e-value: 1.0E-24
score: 87.2
IPR003754Tetrapyrrole biosynthesis, uroporphyrinogen III synthaseCDDcd06578HemDcoord: 34..298
e-value: 1.21376E-24
score: 97.7631
IPR036108Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamilyGENE3D3.40.50.10090coord: 183..304
e-value: 3.7E-15
score: 57.8
IPR036108Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamilySUPERFAMILY69618HemD-likecoord: 33..302
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR38020UROPORPHYRINOGEN-III SYNTHASEcoord: 14..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0033711.1Lag0033711.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006782 protoporphyrinogen IX biosynthetic process
biological_process GO:0033014 tetrapyrrole biosynthetic process
molecular_function GO:0004852 uroporphyrinogen-III synthase activity