Lag0029895 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029895
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	CASP-like protein
Location	chr8: 42933063 .. 42933727 (+)
RNA-Seq Expression	Lag0029895
Synteny	Lag0029895

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAATTCAAGTGGTTCAAGGGTGACTTGTCTTGTATTAAGAATCCTAACGTTTGTGTTTCTTTTCATCTCCTTCTTCATTCTTGTCACAACTTCTCAAACTGTTGCCTCAGTTAAGCTCCATTTCAATAGTTTTCATACCTATAGGTAACTAAATTATGCCTTGTAAATTAATGATTTTCTTTGTAACATCCATCTCTATTCGATTTTACATTTTCTTTTTCTTTTTGGCAGATACGTGCTCGCCACCATCATCATCGGAATCATATTCAACCTCTTGCAAATCGCATTCTCGATTTTTAACATCGTTAAAAGTGGCAGCAACACTATACTATTCGACTTCTTTGGTGACAAGGTACGTTGTAAATTTAGTGGTCATCGAAACGATTATAATCTTGCCACTTTGTATAACAACTTAGCGGATAAAACACTTGTTATAACGAGAAACGTTTTGGTTTGGTGCAGTTTTTGTCCTACCTTTTGGCAACAGGCACAGCAGCTGGATTTGGTCTTTCAGTGGATCTGCACACAACAGATCCAGATGATTACTTTAGATCATTCTTTGATAAGGCTTATGCAGCATCAGCTCTGCTTTTATTTGCATTCTTTTGTGCTGCAGCTGTCTCTATTCTATCTTCCTTTGCTCTCTCCAAGAGAGTTTAA

mRNA sequence

ATGGAAAATTCAAGTGGTTCAAGGGTGACTTGTCTTGTATTAAGAATCCTAACGTTTGTGTTTCTTTTCATCTCCTTCTTCATTCTTGTCACAACTTCTCAAACTGTTGCCTCAGTTAAGCTCCATTTCAATAGTTTTCATACCTATAGATACGTGCTCGCCACCATCATCATCGGAATCATATTCAACCTCTTGCAAATCGCATTCTCGATTTTTAACATCGTTAAAAGTGGCAGCAACACTATACTATTCGACTTCTTTGGTGACAAGTTTTTGTCCTACCTTTTGGCAACAGGCACAGCAGCTGGATTTGGTCTTTCAGTGGATCTGCACACAACAGATCCAGATGATTACTTTAGATCATTCTTTGATAAGGCTTATGCAGCATCAGCTCTGCTTTTATTTGCATTCTTTTGTGCTGCAGCTGTCTCTATTCTATCTTCCTTTGCTCTCTCCAAGAGAGTTTAA

Coding sequence (CDS)

ATGGAAAATTCAAGTGGTTCAAGGGTGACTTGTCTTGTATTAAGAATCCTAACGTTTGTGTTTCTTTTCATCTCCTTCTTCATTCTTGTCACAACTTCTCAAACTGTTGCCTCAGTTAAGCTCCATTTCAATAGTTTTCATACCTATAGATACGTGCTCGCCACCATCATCATCGGAATCATATTCAACCTCTTGCAAATCGCATTCTCGATTTTTAACATCGTTAAAAGTGGCAGCAACACTATACTATTCGACTTCTTTGGTGACAAGTTTTTGTCCTACCTTTTGGCAACAGGCACAGCAGCTGGATTTGGTCTTTCAGTGGATCTGCACACAACAGATCCAGATGATTACTTTAGATCATTCTTTGATAAGGCTTATGCAGCATCAGCTCTGCTTTTATTTGCATTCTTTTGTGCTGCAGCTGTCTCTATTCTATCTTCCTTTGCTCTCTCCAAGAGAGTTTAA

Protein sequence

MENSSGSRVTCLVLRILTFVFLFISFFILVTTSQTVASVKLHFNSFHTYRYVLATIIIGIIFNLLQIAFSIFNIVKSGSNTILFDFFGDKFLSYLLATGTAAGFGLSVDLHTTDPDDYFRSFFDKAYAASALLLFAFFCAAAVSILSSFALSKRV

Homology

[Close]

BLAST of Lag0029895 vs. NCBI nr
Match: XP_022992767.1 (CASP-like protein 4D1 [Cucurbita maxima])

HSP 1 Score: 257.7 bits (657), Expect = 6.5e-65
Identity = 137/154 (88.96%), Postives = 146/154 (94.81%), Query Frame = 0


Query: 1   MENSSGSRVTCLVLRILTFVFLFISFFILVTTSQTVASVKLHFNSFHTYRYVLATIIIGI 60
           MENSSG+RVTCL+LRILTFVFLFISFF+LVTTSQTV SVK HFN+FHTYRYVLATIIIGI
Sbjct: 1   MENSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI 60

Query: 61  IFNLLQIAFSIFNIVKSGSNTILFDFFGDKFLSYLLATGTAAGFGLSVDLHTTDPDDYFR 120
           +FNLLQIAFS+FNIVK  + TILFDFFGDKFLSYLLATGTAAGFGLSVDL T DPDDYF 
Sbjct: 61  VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120

Query: 121 SFFDKAYAASALLLFAFFCAAAVSILSSFALSKR 155
           +FFDKAYAASALLLFAFFC+AAVSILSSFALSKR
Sbjct: 121 TFFDKAYAASALLLFAFFCSAAVSILSSFALSKR 154

[Close]

BLAST of Lag0029895 vs. NCBI nr
Match: XP_023549516.1 (CASP-like protein 4D1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 256.9 bits (655), Expect = 1.1e-64
Identity = 136/154 (88.31%), Postives = 146/154 (94.81%), Query Frame = 0


Query: 1   MENSSGSRVTCLVLRILTFVFLFISFFILVTTSQTVASVKLHFNSFHTYRYVLATIIIGI 60
           MENSSG+RVTCL+LRILTFVFLFISFF+LVTTSQTV SVK HFN+FHTYRYVLATIIIGI
Sbjct: 1   MENSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGI 60

Query: 61  IFNLLQIAFSIFNIVKSGSNTILFDFFGDKFLSYLLATGTAAGFGLSVDLHTTDPDDYFR 120
           +FNLLQIAFS+FNIVK  + TILFDFFGDKFLSYLLATGTAAGFGLSVDL T DPDDYF 
Sbjct: 61  VFNLLQIAFSLFNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120

Query: 121 SFFDKAYAASALLLFAFFCAAAVSILSSFALSKR 155
           +FFDKAYAASALLLFAFFC+AAVSI+SSFALSKR
Sbjct: 121 TFFDKAYAASALLLFAFFCSAAVSIISSFALSKR 154

[Close]

BLAST of Lag0029895 vs. NCBI nr
Match: XP_022939492.1 (CASP-like protein 4D1 [Cucurbita moschata])

HSP 1 Score: 252.3 bits (643), Expect = 2.7e-63
Identity = 133/154 (86.36%), Postives = 145/154 (94.16%), Query Frame = 0


Query: 1   MENSSGSRVTCLVLRILTFVFLFISFFILVTTSQTVASVKLHFNSFHTYRYVLATIIIGI 60
           ME+SSG+RVTCL+LRILTFVFLFISFF+LVTTSQTV SVK HFN+FHTYRYVLATIIIG+
Sbjct: 1   MESSSGARVTCLILRILTFVFLFISFFVLVTTSQTVESVKFHFNNFHTYRYVLATIIIGV 60

Query: 61  IFNLLQIAFSIFNIVKSGSNTILFDFFGDKFLSYLLATGTAAGFGLSVDLHTTDPDDYFR 120
           +FNLLQIAFS+ NIVK  + TILFDFFGDKFLSYLLATGTAAGFGLSVDL T DPDDYF 
Sbjct: 61  VFNLLQIAFSLLNIVKGATGTILFDFFGDKFLSYLLATGTAAGFGLSVDLQTLDPDDYFG 120

Query: 121 SFFDKAYAASALLLFAFFCAAAVSILSSFALSKR 155
           +FFDKAYAASALLLFAFFC+AAVSI+SSFALSKR
Sbjct: 121 TFFDKAYAASALLLFAFFCSAAVSIISSFALSKR 154

[Close]

BLAST of Lag0029895 vs. NCBI nr
Match: XP_038877616.1 (CASP-like protein 4D1 [Benincasa hispida])

HSP 1 Score: 246.5 bits (628), Expect = 1.5e-61
Identity = 132/154 (85.71%), Postives = 144/154 (93.51%), Query Frame = 0


Query: 1   MENSSGSRVTCLVLRILTFVFLFISFFILVTTSQTVASVKLHFNSFHTYRYVLATIIIGI 60
           MENSSGSRVT L+LRILTFVFLFISFFIL+TTSQTV SVKLHFN +H +RY+LATIIIGI
Sbjct: 1   MENSSGSRVTSLILRILTFVFLFISFFILITTSQTVQSVKLHFNYYHAFRYMLATIIIGI 60

Query: 61  IFNLLQIAFSIFNIVKSGSNTILFDFFGDKFLSYLLATGTAAGFGLSVDLHTTDPDDYFR 120
           +FNLLQIAFS+FNIVK+G++TILFDFFGDKFLSYLL TGTAAGFGLSVDL   DPDD+F 
Sbjct: 61  VFNLLQIAFSLFNIVKNGNSTILFDFFGDKFLSYLLGTGTAAGFGLSVDLKKIDPDDFFG 120

Query: 121 SFFDKAYAASALLLFAFFCAAAVSILSSFALSKR 155
           SFFDKAYAAS LLLFAFFC+AAVSILSSFALSKR
Sbjct: 121 SFFDKAYAASTLLLFAFFCSAAVSILSSFALSKR 154

[Close]

BLAST of Lag0029895 vs. NCBI nr
Match: XP_022134358.1 (CASP-like protein 4D1 [Momordica charantia])

HSP 1 Score: 243.4 bits (620), Expect = 1.3e-60
Identity = 132/154 (85.71%), Postives = 140/154 (90.91%), Query Frame = 0


Query: 1   MENSSGSRVTCLVLRILTFVFLFISFFILVTTSQTVASVKLHFNSFHTYRYVLATIIIGI 60
           MENSSGSRVTCL+LRI+TFVFLFISFFILVTTS+TV  V+LHFN FH YRYVLATIIIGI
Sbjct: 1   MENSSGSRVTCLILRIITFVFLFISFFILVTTSKTVMGVRLHFNQFHAYRYVLATIIIGI 60

Query: 61  IFNLLQIAFSIFNIVKSGSNTILFDFFGDKFLSYLLATGTAAGFGLSVDLHTTDPDDYFR 120
           I NLLQIAFS+FN VK+GS TILFDFFGDKFLSYLLATG AAGFGL+VDL TTD DD + 
Sbjct: 61  ILNLLQIAFSLFNTVKNGSGTILFDFFGDKFLSYLLATGMAAGFGLTVDLQTTDADDEYG 120

Query: 121 SFFDKAYAASALLLFAFFCAAAVSILSSFALSKR 155
            FFDKAYAASALLLFAFFCAAAVSILSSFALS R
Sbjct: 121 EFFDKAYAASALLLFAFFCAAAVSILSSFALSNR 154

[Close]

BLAST of Lag0029895 vs. ExPASy Swiss-Prot
Match: C6T1Z6 (CASP-like protein 4D1 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-27
Identity = 77/159 (48.43%), Postives = 105/159 (66.04%), Query Frame = 0


Query: 3   NSSGSRVTCLVLRILTFVFLFISFFILVTTSQT--VASVKLHFNSFHTYRYVLATIIIGI 62
           +S+GSR   L+LR+LTFVFL I+  ++    QT     V++ FN  + YRY+++TIIIG 
Sbjct: 13  SSTGSRTVLLLLRVLTFVFLLIALILIAIVKQTDDETGVEIKFNDIYAYRYMISTIIIGF 72

Query: 63  IFNLLQIAFSIFNIVK-----SGSNTILFDFFGDKFLSYLLATGTAAGFGLSVDLHTTDP 122
            +NLLQ+A SIF +V      SG    LFDFFGDK +SYLL +G+AAGFG++V+L    P
Sbjct: 73  AYNLLQMALSIFTVVSGNRVLSGDGGYLFDFFGDKIISYLLISGSAAGFGVTVELGRGVP 132

Query: 123 DDYFRSFFDKAYAASALLLFAFFCAAAVSILSSFALSKR 155
            +   SF DKA A+++LLL AF   A  S  +SFAL K+
Sbjct: 133 SN---SFMDKANASASLLLIAFLFTAVASTFTSFALPKK 168

[Close]

BLAST of Lag0029895 vs. ExPASy Swiss-Prot
Match: B9SXY8 (CASP-like protein 4D1 OS=Ricinus communis OX=3988 GN=RCOM_1206790 PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.2e-26
Identity = 73/162 (45.06%), Postives = 103/162 (63.58%), Query Frame = 0


Query: 4   SSGSRVTCLVLRILTFVFLFISFFILVTTSQT----VASVKLHFNSFHTYRYVLATIIIG 63
           S  SR+  L+LRILTF+FL  S  IL T + T    +  VK+HF   + YRY+LATI+IG
Sbjct: 7   SLASRMAALILRILTFIFLIASLVILTTNTATLELDLVEVKVHFKDVYAYRYMLATIVIG 66

Query: 64  IIFNLLQIAFSIF-----NIVKSGSNTILFDFFGDKFLSYLLATGTAAGFGLSVDLHTT- 123
           + + +LQIAF+++     N + SG   + FDFFGDK +SY+L TG AAGF  + D+    
Sbjct: 67  LAYTVLQIAFTLYYVATGNRMMSGDGNLAFDFFGDKVISYILVTGAAAGFASTKDIKPVF 126

Query: 124 DPDDYFRSFFDKAYAASALLLFAFFCAAAVSILSSFALSKRV 156
                F +F +K YA+++LLL  F C A +S+ SS+AL K+V
Sbjct: 127 SGSGDFDAFINKGYASASLLLIGFVCTAVLSVFSSYALPKQV 168

[Close]

BLAST of Lag0029895 vs. ExPASy Swiss-Prot
Match: A1XGB4 (CASP-like protein PIMP1 OS=Capsicum annuum OX=4072 GN=PIMP1 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.1e-25
Identity = 70/152 (46.05%), Postives = 99/152 (65.13%), Query Frame = 0


Query: 12  LVLRILTFVFLFISFFILVTTSQTVAS----VKLHFNSFHTYRYVLATIIIGIIFNLLQI 71
           L++RILT + L ISF ++ T +QTV++    VK+ F  F+ YRY++AT+IIG+ + LLQI
Sbjct: 15  LIVRILTLICLLISFIVIATNNQTVSTVAGDVKIKFKDFYAYRYLIATVIIGMAYTLLQI 74

Query: 72  AFSIFNIVK----SGSNTILFDFFGDKFLSYLLATGTAAGFGLSVDLHTTDPDDYFRSFF 131
           AFSI  +       G   +LFDF+GDKF+SY L TG AA FG++ DL   +  D +  F 
Sbjct: 75  AFSISLLTTGNRIGGEGFLLFDFYGDKFISYFLVTGAAASFGMTQDLKQLEGSDNYSKFL 134

Query: 132 DKAYAASALLLFAFFCAAAVSILSSFALSKRV 156
           + + AA++L L  FF A A SI SS+ L KR+
Sbjct: 135 NTSNAAASLCLIGFFFAVASSIFSSYNLPKRI 166

[Close]

BLAST of Lag0029895 vs. ExPASy Swiss-Prot
Match: Q56X75 (CASP-like protein 4D2 OS=Arabidopsis thaliana OX=3702 GN=At2g39518 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.4e-24
Identity = 70/160 (43.75%), Postives = 101/160 (63.12%), Query Frame = 0


Query: 8   RVTCLVLRILTFVFLFISFFILVTTSQTVAS----VKLHFNSFHTYRYVLATIIIGIIFN 67
           +V  L+LR+LT VFL I+  IL T S T+ S    +K HF   + YRY+L+  +IG+++ 
Sbjct: 14  KVLLLLLRVLTGVFLVIALIILSTNSVTIVSQGSALKFHFKDVYAYRYMLSAAVIGLVYA 73

Query: 68  LLQIAFSIFNIVKSGSNTILF--DFFGDKFLSYLLATGTAAGFGLSVDLHTT-------D 127
           ++Q+ F+I        N   +  DF+GDK +SYL+ATG+AAGFG++ DL  T       D
Sbjct: 74  VIQLFFTISEFATGVKNPFNYQLDFYGDKLISYLVATGSAAGFGVTKDLKDTFLALVALD 133

Query: 128 PDDYFRSFFDKAYAASALLLFAFFCAAAVSILSSFALSKR 155
             D    FF K YA+++LLLFAF C A +S+ SSFA++KR
Sbjct: 134 STDPVDKFFSKGYASASLLLFAFICLAVLSVFSSFAMAKR 173

[Close]

BLAST of Lag0029895 vs. ExPASy Swiss-Prot
Match: B9NBE5 (CASP-like protein 4D1 OS=Populus trichocarpa OX=3694 GN=POPTR_0010s21230g PE=2 SV=2)

HSP 1 Score: 109.4 bits (272), Expect = 3.7e-23
Identity = 68/159 (42.77%), Postives = 99/159 (62.26%), Query Frame = 0


Query: 7   SRVTCLVLRILTFVFLFISFFILVTTSQTVA----SVKLHFNSFHTYRYVLATIIIGIIF 66
           SR+T L LR+LTF FL +S  I+ T + T+       K+    F++YRY+LA I  G+ +
Sbjct: 19  SRMTALFLRVLTFAFLMVSLVIMTTNTGTIEIGIDEFKVRSKDFYSYRYMLAAIAFGLTY 78

Query: 67  NLLQIAFSIFNIVK------SGSNTILFDFFGDKFLSYLLATGTAAGFGLSVDLHTTDPD 126
            +LQIA ++ +I K      SG   ++FDF+GDK +SY+LATG AA FG + +L T    
Sbjct: 79  TILQIALTLNHISKRNGAQTSGDGNLVFDFYGDKVVSYILATGAAAAFGATKELKTQLAG 138

Query: 127 DYFRSFFDKAYAASALLLFAFFCAAAVSILSSFALSKRV 156
                FF+K YA+++LLL  F C A +S+ SS+AL K+V
Sbjct: 139 LGGDKFFNKGYASASLLLLGFVCTAILSVFSSYALPKKV 177

The following BLAST results are available for this feature:

BLAST of Lag0029895 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022992767.1	6.5e-65	88.96	CASP-like protein 4D1 [Cucurbita maxima]	[more]
XP_023549516.1	1.1e-64	88.31	CASP-like protein 4D1 [Cucurbita pepo subsp. pepo]	[more]
XP_022939492.1	2.7e-63	86.36	CASP-like protein 4D1 [Cucurbita moschata]	[more]
XP_038877616.1	1.5e-61	85.71	CASP-like protein 4D1 [Benincasa hispida]	[more]
XP_022134358.1	1.3e-60	85.71	CASP-like protein 4D1 [Momordica charantia]	[more]

BLAST of Lag0029895 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C6T1Z6	1.1e-27	48.43	CASP-like protein 4D1 OS=Glycine max OX=3847 PE=2 SV=1	[more]
B9SXY8	1.2e-26	45.06	CASP-like protein 4D1 OS=Ricinus communis OX=3988 GN=RCOM_1206790 PE=3 SV=1	[more]
A1XGB4	3.1e-25	46.05	CASP-like protein PIMP1 OS=Capsicum annuum OX=4072 GN=PIMP1 PE=2 SV=1	[more]
Q56X75	3.4e-24	43.75	CASP-like protein 4D2 OS=Arabidopsis thaliana OX=3702 GN=At2g39518 PE=2 SV=1	[more]
B9NBE5	3.7e-23	42.77	CASP-like protein 4D1 OS=Populus trichocarpa OX=3694 GN=POPTR_0010s21230g PE=2 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006702	Casparian strip membrane protein domain	PFAM	PF04535	DUF588	coord: 6..139 e-value: 1.4E-20 score: 73.6
None	No IPR available	PANTHER	PTHR11615:SF148	CASP-LIKE PROTEIN 4D2	coord: 5..155
None	No IPR available	PANTHER	PTHR11615	NITRATE, FORMATE, IRON DEHYDROGENASE	coord: 5..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0029895.1	Lag0029895.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding