Lag0029379 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0029379 vs. NCBI nr
Match: XP_022923630.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 1057.7 bits (2734), Expect = 3.5e-305
Identity = 512/576 (88.89%), Postives = 542/576 (94.10%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M  LSKCDANYASLTPITFL+RAS+FY+DRTSIIY+GTRFTWRQTYQRCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           + KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDVNNIA ILRHSEAKILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVKVDDE 180
           +IQ+AK ALR L+A S P+P VVVIDDIDSPTGARFG LEYEQL+H+GD GF PV+VDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGWPVNV 300
           WGIAARGGTNVCMRNTTA DI+RNINLHHVTHMCCAPIVFRIIL+A+GG+RRR GW VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDGRTTGEIVLRGSSIMKGYFKDPAATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVH--GGGMSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKL 540
           CAFV VH  GG +SEAE+I FCR+N+SHYM PKKVVFVAELPKT TGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 FAISEKRVVGGNKSGRQDGSRRPDYDGEHVLAMSRL 575
              SEKRV GGNKSGR DG R+PDY G+HV+AMSRL
Sbjct: 541 LVGSEKRVSGGNKSGRHDGGRQPDY-GQHVMAMSRL 575

BLAST of Lag0029379 vs. NCBI nr
Match: KAG6602004.1 (Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1053.9 bits (2724), Expect = 5.0e-304
Identity = 511/576 (88.72%), Postives = 541/576 (93.92%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M  LSKCDANYASLTPITFL+RAS+FY+DRTSIIY+GTRFTWRQTYQRCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           + KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDVNNIA ILRHSEAKILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVKVDDE 180
           +IQ+AK ALR L+A S P+P VVVIDDIDSPTGARFG LEYEQL+H+GD GF PV+VDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGWPVNV 300
           WGIAARGGTNVCMRNTTA DI+RNINLHHVTHMCCAPIVFRIIL+A+GG+RRR GW VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDGRTTGEIVLRGSSIMKGYFKD AATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDLAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVH--GGGMSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKL 540
           CAFV VH  GG +SEAE+I FCR+N+SHYM PKKVVFVAELPKT TGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 FAISEKRVVGGNKSGRQDGSRRPDYDGEHVLAMSRL 575
              SEKRV GGNKSGR DG R+PDY G+HV+AMSRL
Sbjct: 541 LVGSEKRVSGGNKSGRHDGGRQPDY-GQHVMAMSRL 575

BLAST of Lag0029379 vs. NCBI nr
Match: XP_023513711.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.5e-303
Identity = 508/576 (88.19%), Postives = 542/576 (94.10%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M  LSKCDANYASLTPITFL+RAS+FY+DRTSIIY+GTRFTWRQTY+RCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLSRLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           + KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV+NIA ILRHSEAKILFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVKVDDE 180
           +IQ+AK ALR L+A S P+P VVVIDDIDSPTGARFG LEYEQL+H+GD GF PV+VDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLV+GWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVVGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGWPVNV 300
           WGIAARGGTNVCMRNTTA DI+RNINLHHVTHMCCAPIVFRIIL+A+GG+RRR GW VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDGRTTGEIVLRGSSIMKGYFKDPAATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVH--GGGMSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKL 540
           CAFV VH  GG +SEAE+I FCR+N+SHYM PKKVVFVAELPKT TGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 FAISEKRVVGGNKSGRQDGSRRPDYDGEHVLAMSRL 575
              SEKRV GGNKSGR DG R+P+Y G+HV+AMSRL
Sbjct: 541 LVGSEKRVSGGNKSGRHDGGRQPEY-GQHVMAMSRL 575

BLAST of Lag0029379 vs. NCBI nr
Match: XP_022990938.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 1043.5 bits (2697), Expect = 6.7e-301
Identity = 504/576 (87.50%), Postives = 538/576 (93.40%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M  LSKCDAN+ASLTPITFL+RAS+FY+DRTSIIY+GT FTWRQTY+RCC LASSL RLN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           + KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV+NIA ILRHSEAKILFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVKVDDE 180
           +IQ+AK ALR L+A S P+P VVVIDDIDSPTGARFG LEYEQL+H+GD GF PV+VDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGWPVNV 300
           WGIAARGGTNVC+RNTTA DI+RNINLHHVTHMCCAPIVFRIIL+A+GG+RRR GW VNV
Sbjct: 241 WGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDGRTTGEIVLRGSSIMKGYFKDPAATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVH--GGGMSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKL 540
           CAFV VH   G +SEAE+I FCR+N+SHYM PKKV+FVAELPKT TGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 FAISEKRVVGGNKSGRQDGSRRPDYDGEHVLAMSRL 575
              SEKRV GGNKSGR DG R P+Y GEHV+AMSRL
Sbjct: 541 LVASEKRVSGGNKSGRHDGGRLPEY-GEHVMAMSRL 575

BLAST of Lag0029379 vs. NCBI nr
Match: XP_038906606.1 (trans-cinnamate:CoA ligase, peroxisomal-like [Benincasa hispida])

HSP 1 Score: 1041.6 bits (2692), Expect = 2.6e-300
Identity = 508/578 (87.89%), Postives = 538/578 (93.08%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M+ L KCDANY SLTPITFL RAS+FY+DRTSIIY+GTRFTW QTY+RCCRLASSL  LN
Sbjct: 1   MDMLLKCDANYTSLTPITFLTRASTFYADRTSIIYEGTRFTWHQTYERCCRLASSLRHLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           I+KNDVVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDVNNIA ILRHSEAKILFVDYQ
Sbjct: 61  ISKNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIAVILRHSEAKILFVDYQ 120

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVKVDDE 180
           YIQ+AK ALRLL+A+SFP+PLVV+IDDIDSPTG RFGN EYE+LIH+GD  F P++VDDE
Sbjct: 121 YIQEAKDALRLLMAKSFPMPLVVIIDDIDSPTGIRFGNFEYEKLIHDGDVNFTPLEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGWPVNV 300
           WGIAARGGTN+CMRNTTA+DIFRNINLHHVTHMCCAPIVFRIILDA+  DR RV WP+NV
Sbjct: 241 WGIAARGGTNICMRNTTAIDIFRNINLHHVTHMCCAPIVFRIILDANSHDRCRVAWPINV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLKARQG 360
           LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN LPAD+QAKLKARQG
Sbjct: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNTLPADKQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNLDTMESVPHDG TTGEIVLRGS +MKGYFKDP ATAAA KNGWFLTG
Sbjct: 361 ISILTLADVDVKNLDTMESVPHDGHTTGEIVLRGSGLMKGYFKDPVATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLY HPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYSHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVHG--GGMSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKL 540
           CAFVAVHG  G +SEAEIIAFCR+NL HYMVPKKVVFV ELPK + GKIQKFKLREQAK 
Sbjct: 481 CAFVAVHGDAGRVSEAEIIAFCRKNLPHYMVPKKVVFVGELPK-ANGKIQKFKLREQAKY 540

Query: 541 FAISEKRVV--GGNKSGRQDGSRRPDYDGEHVLAMSRL 575
             +SEKRVV  G NKS RQDG+RR DY GEHVLAMSRL
Sbjct: 541 LEVSEKRVVGTGWNKSSRQDGNRRSDY-GEHVLAMSRL 576

BLAST of Lag0029379 vs. ExPASy Swiss-Prot
Match: I3PB36 (Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 3.7e-217
Identity = 367/553 (66.37%), Postives = 430/553 (77.76%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M++L KC ANY  LTP+TFL RA   Y++RTSIIY G RFTW QTY+RCCRLASSL  LN
Sbjct: 1   MDELPKCGANYVPLTPLTFLTRAFKSYANRTSIIYAGARFTWEQTYKRCCRLASSLQSLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           I KNDVVSVLAPNVPA YEMHFAVPMAGAVLNTINTRLD  NIA IL+HSEAK+LFVDY+
Sbjct: 61  IVKNDVVSVLAPNVPATYEMHFAVPMAGAVLNTINTRLDPMNIAIILKHSEAKLLFVDYE 120

Query: 121 YIQQAKSALRLLVAESF---------PIPLVVVIDDIDSPTG-ARFGNLEYEQLIHNGDA 180
           Y+++A+ AL LL++ +F          +P V++IDD+ SPT   +   LEYEQL+H G+ 
Sbjct: 121 YLEKARKALELLMSTNFITAQNSKKISMPQVILIDDLYSPTRIQQQDQLEYEQLVHQGNP 180

Query: 181 GFVPVK-VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTL 240
            + P   VDDEWD I LNYTSGTTS PKGVVYSHRGAFLSTL+ +MGWEMG  PVYLW+L
Sbjct: 181 EYAPENIVDDEWDPIVLNYTSGTTSEPKGVVYSHRGAFLSTLNTIMGWEMGTEPVYLWSL 240

Query: 241 PMFHCNGWTFTWGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGG 300
           PMFH NGWT TWGIAARGGTNVC+RNTTA +I+ NI LH VTHMCCAP VF I+L+A   
Sbjct: 241 PMFHINGWTLTWGIAARGGTNVCIRNTTAQEIYSNITLHKVTHMCCAPTVFNILLEAKPH 300

Query: 301 DRRRVGWPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPA 360
           +RR +  PV V+ GGAPPP  L+ K+E LGFH+ H YG+TEA G  LVCEWQ +WN L  
Sbjct: 301 ERREITTPVQVMVGGAPPPTTLIGKIEELGFHVVHCYGITEAGGTTLVCEWQSEWNKLSR 360

Query: 361 DEQAKLKARQGISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATA 420
           ++QA LKARQGIS+L L DVDVKN  TM+SVPH+G+T GEI LRGSSIMKGYFK+  A +
Sbjct: 361 EDQANLKARQGISVLALEDVDVKNSKTMQSVPHNGKTMGEICLRGSSIMKGYFKNDKANS 420

Query: 421 AALKNGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVV 480
              KNGWFLTGDV VIH DGYLEIKDR KD+IISGGENISS+E+E  + +HP V EAAVV
Sbjct: 421 QVFKNGWFLTGDVAVIHQDGYLEIKDRCKDIIISGGENISSIEVENAILKHPSVIEAAVV 480

Query: 481 AMPHPRWGESPCAFV-AVHGGGMSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQ 540
           AMPHPRWGE+PCAFV       + EA+II  C++ L  +MVPK V F+ ELPKT TGK++
Sbjct: 481 AMPHPRWGETPCAFVIKTKNPEIKEADIIVHCKKELPGFMVPKHVQFLEELPKTGTGKVK 540

Query: 541 KFKLREQAKLFAI 542
           K +LRE AK F I
Sbjct: 541 KLQLREMAKSFGI 553

BLAST of Lag0029379 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 3.7e-201
Identity = 342/552 (61.96%), Postives = 417/552 (75.54%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M+ L  C+AN   LTPITFL+RAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           IT+NDVVS+LAPNVPA+YEMHF+VPM GAVLN INTRLD   IA ILRH+E KILFVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIQQAKSALRLL-VAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGD----AGFVPV 180
           +    +  LRL+   +S P P +++I++IDS T      L+YE LI  G+    +     
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 KVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
           +V +E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVG 300
           GWT TW +AARGGTNVC+R+ TA +I++NI LH VTHM C P VFR +L+    D+    
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 WPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKL 360
            PV VLTGG+ PPA L++K+E LGFH+ H YGLTEATGP L CEWQ++WN LP  +Q +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           + RQG+  LTLADVDVKN  T+ESVP DG+T GEIV++GSS+MKGY K+P AT+ A K+G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGD+GVIHPDGY+EIKDRSKD+IISGGENISS+E+E VLY + +V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFVAVHGGG----MSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFK 540
           WGE+PCAFV +  G      SE ++I +CREN+ H+M PKKVVF  ELPK S GKI K K
Sbjct: 481 WGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSK 540

Query: 541 LREQAKLFAISE 544
           LR+ AK   + E
Sbjct: 541 LRDIAKALVVRE 552

BLAST of Lag0029379 vs. ExPASy Swiss-Prot
Match: Q9SS01 (Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 2.6e-199
Identity = 343/561 (61.14%), Postives = 420/561 (74.87%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M+ L+ C+AN   LTP+TFL+RAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           I+KNDVVSV+APN PALYEMHFAVPMAGAVLN INTRLD  +IA ILRH++ KILF+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIQQAKSALRLLVAESFPIPL-VVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPV---- 180
           +   A+ +L LL +E   + L V+ I + D P  A F  L+YE LI  G+     V    
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 KVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
           ++ DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVG 300
           GWTFTWG AARGGT+VCMR+ TA +I++NI +H+VTHMCC P VF I+L  +  D     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 WPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKL 360
            PV+VLTGG+PPPA L++K++ LGF + HAYG TEATGP L CEWQ++WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           KARQGISIL LADVDVKN +T +S P DG+T GEI+++GSSIMKGY K+P AT  A K+G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E VLY++P+V E AVVAMPHP 
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAVHGGGMS------------EAEIIAFCRENLSHYMVPKKVVFVAELPKTS 540
           WGE+PCAFV +     +            E  +I +CRENL H+M P+KVVF+ ELPK  
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540

Query: 541 TGKIQKFKLREQAKLFAISEK 545
            GKI K KLR+ AK   + ++
Sbjct: 541 NGKILKPKLRDIAKGLVVEDE 561

BLAST of Lag0029379 vs. ExPASy Swiss-Prot
Match: Q9SS00 (Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE12 PE=2 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 1.1e-197
Identity = 338/553 (61.12%), Postives = 417/553 (75.41%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M+ L+ C+AN   LTPITFL+RAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           I KNDVVSV+APN PA+YEMHFAVPMAGAVLN INTRLD  +IA ILRH++ KILF+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIQQAKSALRLLVAESFPIPL-VVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVK--- 180
           +   A+  L+LL +E   + L V+ I +ID P        +YE LI  G+   + +    
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARMF 180

Query: 181 -VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 CIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVG 300
           GWTFTWG AARGGT+VCMR+ TA +I++NI +H+VTHMCC P VF I+L  +  D     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 300

Query: 301 WPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKL 360
            PV+VLTGG+PPPA L++K++ LGF + HAYGLTEATGP L CEWQ++WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           KARQG+SIL L +VDV+N +T ESVP DG+T GEIV++GSSIMKGY K+P AT  A K+G
Sbjct: 361 KARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  +GDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E ++Y++P+V E AVVAMPHP 
Sbjct: 421 WLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAVHGGGMS-----------EAEIIAFCRENLSHYMVPKKVVFVAELPKTST 538
           WGE+PCAFV +  G  +           E ++I +CRENL H+M P+KVVF+ ELPK   
Sbjct: 481 WGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNGN 540

BLAST of Lag0029379 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 7.0e-168
Identity = 286/550 (52.00%), Postives = 397/550 (72.18%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           ME   K  ANY  LTPI+FL R++  Y+DR SI+Y   ++TWRQT  RC R+AS+L +L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           I+  DVVSVLAPNVPA+ E+HF VPMAGA+L T+N R D + +A +LRHS  K++F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGN----LEYEQLIHNGDAGFVPVK 180
           ++Q A+ A  +L  +   +P++V+I +  + + +R       +EYE ++  G + F  ++
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGW 300
           W   WG+ A GGTN+C+RN TA  IF NI+ H VTHM  AP +  +I++A   +++ +  
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 PVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLK 360
            V+ +TG APPPA ++ KME LGF + H+YGLTE  GP  +C W+ +W+ LP +EQAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGW 420
           ARQG++ L L ++ VK+  TM ++P DG T GE+V RG+++M GY K+P AT  A K GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRW 480
           F +GD+GV HPDGY+E+KDRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFVAVHGGGMSEA-EIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
           GE+ CAFV +  G  + A E+I++CR+ L HYM P+ +VF  +LPKTSTGK+QKF LR +
Sbjct: 483 GETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRTK 542

Query: 541 AK-LFAISEK 545
           AK L ++S+K
Sbjct: 543 AKALVSLSKK 551

BLAST of Lag0029379 vs. ExPASy TrEMBL
Match: A0A6J1ECD9 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431279 PE=4 SV=1)

HSP 1 Score: 1057.7 bits (2734), Expect = 1.7e-305
Identity = 512/576 (88.89%), Postives = 542/576 (94.10%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M  LSKCDANYASLTPITFL+RAS+FY+DRTSIIY+GTRFTWRQTYQRCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           + KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDVNNIA ILRHSEAKILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVKVDDE 180
           +IQ+AK ALR L+A S P+P VVVIDDIDSPTGARFG LEYEQL+H+GD GF PV+VDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGWPVNV 300
           WGIAARGGTNVCMRNTTA DI+RNINLHHVTHMCCAPIVFRIIL+A+GG+RRR GW VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDGRTTGEIVLRGSSIMKGYFKDPAATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVH--GGGMSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKL 540
           CAFV VH  GG +SEAE+I FCR+N+SHYM PKKVVFVAELPKT TGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 FAISEKRVVGGNKSGRQDGSRRPDYDGEHVLAMSRL 575
              SEKRV GGNKSGR DG R+PDY G+HV+AMSRL
Sbjct: 541 LVGSEKRVSGGNKSGRHDGGRQPDY-GQHVMAMSRL 575

BLAST of Lag0029379 vs. ExPASy TrEMBL
Match: A0A6J1JUR4 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111487678 PE=4 SV=1)

HSP 1 Score: 1043.5 bits (2697), Expect = 3.3e-301
Identity = 504/576 (87.50%), Postives = 538/576 (93.40%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M  LSKCDAN+ASLTPITFL+RAS+FY+DRTSIIY+GT FTWRQTY+RCC LASSL RLN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           + KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV+NIA ILRHSEAKILFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVKVDDE 180
           +IQ+AK ALR L+A S P+P VVVIDDIDSPTGARFG LEYEQL+H+GD GF PV+VDDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGWPVNV 300
           WGIAARGGTNVC+RNTTA DI+RNINLHHVTHMCCAPIVFRIIL+A+GG+RRR GW VNV
Sbjct: 241 WGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWN+LPADEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+SVPHDGRTTGEIVLRGSSIMKGYFKDPAATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVH--GGGMSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKL 540
           CAFV VH   G +SEAE+I FCR+N+SHYM PKKV+FVAELPKT TGKIQKFKLREQAKL
Sbjct: 481 CAFVVVHSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLREQAKL 540

Query: 541 FAISEKRVVGGNKSGRQDGSRRPDYDGEHVLAMSRL 575
              SEKRV GGNKSGR DG R P+Y GEHV+AMSRL
Sbjct: 541 LVASEKRVSGGNKSGRHDGGRLPEY-GEHVMAMSRL 575

BLAST of Lag0029379 vs. ExPASy TrEMBL
Match: A0A5A7TXW0 (Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold284G00160 PE=4 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 5.4e-296
Identity = 500/578 (86.51%), Postives = 534/578 (92.39%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           ME L KCDANY +LTPITFL+RAS+FY+DRTSIIY+GTRFTW QTY+RCCRLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           ++KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV NIA ILRHSEAKI FVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVKVDDE 180
           YIQ+AK ALRLLVAESF IPLVVVIDDID+PTGAR GNLEYEQLIH+GDA F PV+VDDE
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGWPVNV 300
           WGIAARGGTN+CMRNTTA DIFRNINLH VTHMCCAPIVFRIIL+AD GDRRRV WPVNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPAD+QA LKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL TMESVPHDGRTTGEIVLRGSS+MKGYFKDP ATAAA KNGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVHGGG----MSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQA 540
           CAFV +HGGG    +SEAE+IAFCR+NLSHYMVPKKVVF+AELPK + GK+QKF LREQA
Sbjct: 481 CAFVVLHGGGGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLREQA 540

Query: 541 KLFAISEKRVVGGNKSGRQDGSRRPDYDGEHVLAMSRL 575
           K   +SEKR V  +  G + G+RR DY  +HV+AMSRL
Sbjct: 541 KYLEVSEKRRV--SNLGNKSGNRRSDYGDQHVIAMSRL 575

BLAST of Lag0029379 vs. ExPASy TrEMBL
Match: A0A1S3CIM1 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103501376 PE=4 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 5.4e-296
Identity = 500/578 (86.51%), Postives = 534/578 (92.39%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           ME L KCDANY +LTPITFL+RAS+FY+DRTSIIY+GTRFTW QTY+RCCRLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           ++KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV NIA ILRHSEAKI FVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVKVDDE 180
           YIQ+AK ALRLLVAESF IPLVVVIDDID+PTGAR GNLEYEQLIH+GDA F PV+VDDE
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGWPVNV 300
           WGIAARGGTN+CMRNTTA DIFRNINLH VTHMCCAPIVFRIIL+AD GDRRRV WPVNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLKARQG 360
           LTGGAPPPA LLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPAD+QA LKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 ISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL TMESVPHDGRTTGEIVLRGSS+MKGYFKDP ATAAA KNGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAVHGGG----MSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQA 540
           CAFV +HGGG    +SEAE+IAFCR+NLSHYMVPKKVVF+AELPK + GK+QKF LREQA
Sbjct: 481 CAFVVLHGGGGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLREQA 540

Query: 541 KLFAISEKRVVGGNKSGRQDGSRRPDYDGEHVLAMSRL 575
           K   +SEKR V  +  G + G+RR DY  +HV+AMSRL
Sbjct: 541 KYLEVSEKRRV--SNLGNKSGNRRSDYGDQHVIAMSRL 575

BLAST of Lag0029379 vs. ExPASy TrEMBL
Match: A0A0A0KT40 (Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G319910 PE=4 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 2.0e-295
Identity = 497/575 (86.43%), Postives = 536/575 (93.22%), Query Frame = 0

Query: 4   LSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLNITK 63
           L KCDANY++LTPITFL+RAS+FY+DRTSIIY+GTRFTW+QTY+RCCRLASSLCRL ++K
Sbjct: 2   LLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLTVSK 61

Query: 64  NDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQYIQ 123
           NDVVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD+ NIA ILRHSEAKI FVDYQYIQ
Sbjct: 62  NDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQYIQ 121

Query: 124 QAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVKVDDEWDS 183
           +AK ALRLLVAES PIPLVVVIDDID+PTGAR GNLEYEQLI++GDA F PV+VDDEWDS
Sbjct: 122 EAKDALRLLVAESSPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDEWDS 181

Query: 184 IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI 243
           IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI
Sbjct: 182 IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI 241

Query: 244 AARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGWPVNVLTG 303
           AARGGTN+CMRNTTA DIFRNINLH VTHMCCAPIVF IIL+AD  DRRRV WPVNVLTG
Sbjct: 242 AARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNVLTG 301

Query: 304 GAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLKARQGISI 363
           GAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPAD+QA LKARQGISI
Sbjct: 302 GAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQGISI 361

Query: 364 LTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTGDVG 423
           LTLADVDVKNL TMESVPHDGRTTGEIVLRGSS+MKGYFKDPAATAAALKNGWFLTGD+G
Sbjct: 362 LTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPAATAAALKNGWFLTGDIG 421

Query: 424 VIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESPCAF 483
           VI+PDGY+EIKDRSKDVIISGGENISSVE+ETVLYRHPQV+EAAVVAMPHPRWGESPCAF
Sbjct: 422 VIYPDGYVEIKDRSKDVIISGGENISSVEVETVLYRHPQVSEAAVVAMPHPRWGESPCAF 481

Query: 484 VAVHGGG----MSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQAKLF 543
           V +  GG    +SEAE++AFCR+NLSHYMVPKKVVFVAELPK + GK+QKF LREQAK  
Sbjct: 482 VVLQDGGGSGRVSEAEMVAFCRKNLSHYMVPKKVVFVAELPK-ANGKVQKFMLREQAKYL 541

Query: 544 AISEKRVVGGNKSGRQDGSRRPDYDGEHVLAMSRL 575
            +SEKR+V  +  G + G+RR DY GEHV+AMSRL
Sbjct: 542 EVSEKRIV--SNMGNKSGNRRSDY-GEHVIAMSRL 572

BLAST of Lag0029379 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 702.6 bits (1812), Expect = 2.6e-202
Identity = 342/552 (61.96%), Postives = 417/552 (75.54%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M+ L  C+AN   LTPITFL+RAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           IT+NDVVS+LAPNVPA+YEMHF+VPM GAVLN INTRLD   IA ILRH+E KILFVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIQQAKSALRLL-VAESFPIPLVVVIDDIDSPTGARFGNLEYEQLIHNGD----AGFVPV 180
           +    +  LRL+   +S P P +++I++IDS T      L+YE LI  G+    +     
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 KVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
           +V +E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVG 300
           GWT TW +AARGGTNVC+R+ TA +I++NI LH VTHM C P VFR +L+    D+    
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 WPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKL 360
            PV VLTGG+ PPA L++K+E LGFH+ H YGLTEATGP L CEWQ++WN LP  +Q +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           + RQG+  LTLADVDVKN  T+ESVP DG+T GEIV++GSS+MKGY K+P AT+ A K+G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGD+GVIHPDGY+EIKDRSKD+IISGGENISS+E+E VLY + +V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFVAVHGGG----MSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFK 540
           WGE+PCAFV +  G      SE ++I +CREN+ H+M PKKVVF  ELPK S GKI K K
Sbjct: 481 WGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKSK 540

Query: 541 LREQAKLFAISE 544
           LR+ AK   + E
Sbjct: 541 LRDIAKALVVRE 552

BLAST of Lag0029379 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 696.4 bits (1796), Expect = 1.9e-200
Identity = 343/561 (61.14%), Postives = 420/561 (74.87%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M+ L+ C+AN   LTP+TFL+RAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           I+KNDVVSV+APN PALYEMHFAVPMAGAVLN INTRLD  +IA ILRH++ KILF+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIQQAKSALRLLVAESFPIPL-VVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPV---- 180
           +   A+ +L LL +E   + L V+ I + D P  A F  L+YE LI  G+     V    
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 KVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
           ++ DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVG 300
           GWTFTWG AARGGT+VCMR+ TA +I++NI +H+VTHMCC P VF I+L  +  D     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 WPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKL 360
            PV+VLTGG+PPPA L++K++ LGF + HAYG TEATGP L CEWQ++WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           KARQGISIL LADVDVKN +T +S P DG+T GEI+++GSSIMKGY K+P AT  A K+G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E VLY++P+V E AVVAMPHP 
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAVHGGGMS------------EAEIIAFCRENLSHYMVPKKVVFVAELPKTS 540
           WGE+PCAFV +     +            E  +I +CRENL H+M P+KVVF+ ELPK  
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540

Query: 541 TGKIQKFKLREQAKLFAISEK 545
            GKI K KLR+ AK   + ++
Sbjct: 541 NGKILKPKLRDIAKGLVVEDE 561

BLAST of Lag0029379 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 691.0 bits (1782), Expect = 7.9e-199
Identity = 338/553 (61.12%), Postives = 417/553 (75.41%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           M+ L+ C+AN   LTPITFL+RAS  Y +RTSIIY  TRFTW QTY RCCRLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           I KNDVVSV+APN PA+YEMHFAVPMAGAVLN INTRLD  +IA ILRH++ KILF+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIQQAKSALRLLVAESFPIPL-VVVIDDIDSPTGARFGNLEYEQLIHNGDAGFVPVK--- 180
           +   A+  L+LL +E   + L V+ I +ID P        +YE LI  G+   + +    
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARMF 180

Query: 181 -VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 CIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVG 300
           GWTFTWG AARGGT+VCMR+ TA +I++NI +H+VTHMCC P VF I+L  +  D     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 300

Query: 301 WPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKL 360
            PV+VLTGG+PPPA L++K++ LGF + HAYGLTEATGP L CEWQ++WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           KARQG+SIL L +VDV+N +T ESVP DG+T GEIV++GSSIMKGY K+P AT  A K+G
Sbjct: 361 KARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  +GDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E ++Y++P+V E AVVAMPHP 
Sbjct: 421 WLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAVHGGGMS-----------EAEIIAFCRENLSHYMVPKKVVFVAELPKTST 538
           WGE+PCAFV +  G  +           E ++I +CRENL H+M P+KVVF+ ELPK   
Sbjct: 481 WGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNGN 540

BLAST of Lag0029379 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 592.0 bits (1525), Expect = 5.0e-169
Identity = 286/550 (52.00%), Postives = 397/550 (72.18%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIYDGTRFTWRQTYQRCCRLASSLCRLN 60
           ME   K  ANY  LTPI+FL R++  Y+DR SI+Y   ++TWRQT  RC R+AS+L +L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDYQ 120
           I+  DVVSVLAPNVPA+ E+HF VPMAGA+L T+N R D + +A +LRHS  K++F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIQQAKSALRLLVAESFPIPLVVVIDDIDSPTGARFGN----LEYEQLIHNGDAGFVPVK 180
           ++Q A+ A  +L  +   +P++V+I +  + + +R       +EYE ++  G + F  ++
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRRVGW 300
           W   WG+ A GGTN+C+RN TA  IF NI+ H VTHM  AP +  +I++A   +++ +  
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 PVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQAKLK 360
            V+ +TG APPPA ++ KME LGF + H+YGLTE  GP  +C W+ +W+ LP +EQAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALKNGW 420
           ARQG++ L L ++ VK+  TM ++P DG T GE+V RG+++M GY K+P AT  A K GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRW 480
           F +GD+GV HPDGY+E+KDRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFVAVHGGGMSEA-EIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKFKLREQ 540
           GE+ CAFV +  G  + A E+I++CR+ L HYM P+ +VF  +LPKTSTGK+QKF LR +
Sbjct: 483 GETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKFVLRTK 542

Query: 541 AK-LFAISEK 545
           AK L ++S+K
Sbjct: 543 AKALVSLSKK 551

BLAST of Lag0029379 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 578.9 bits (1491), Expect = 4.4e-165
Identity = 291/547 (53.20%), Postives = 380/547 (69.47%), Query Frame = 0

Query: 1   MEKLSKCDANYASLTPITFLQRASSFYSDRTSIIY-DGTRFTWRQTYQRCCRLASSLCRL 60
           ME++  C AN   LTPI FL+RA++ Y D TSI+Y   T +TWR+T  RC R+ASSL  +
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  NITKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVNNIATILRHSEAKILFVDY 120
            I ++DVVSVL+PN PA+YE+ FAVPM+GA+LN INTRLD   ++ +LRH  +K+LFVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 QYIQQAKSALRLLVAESFPIPLVVVID------DIDSPTGARFGNLEYEQLIHNGDAGFV 180
             +  A  A+ ++  +  P  LV + D      D D     +F +  Y+ LIH GD  F 
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIADKEEEGGDADVADRTKF-SYTYDDLIHRGDLDFK 180

Query: 181 PVKVDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFH 240
            ++ + EWD + LNYTSGTTSAPKGVV+ HRG F+ ++  ++ W +   PVYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 CNGWTFTWGIAARGGTNVCMRNTTAVDIFRNINLHHVTHMCCAPIVFRIILDADGGDRRR 300
            NGW++ WGIAA GGTNVC+R   A  I+R I  H VTHMC AP+V  ++  +   + + 
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNML--SATNEFQP 300

Query: 301 VGWPVNVLTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADEQA 360
           +  PVN+LT GAPPPA +L + E++GF I+H YGLTE  G  + C W+ +WN LPA ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 KLKARQGISILTLADVDVKNLDTMESVPHDGRTTGEIVLRGSSIMKGYFKDPAATAAALK 420
           +LKARQG+  +   ++DV + ++  SV  +G T GEIV+RGSSIM GY KDP  T  ALK
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALK 420

Query: 421 NGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPH 480
           NGWF TGDVGVIH DGYLEIKDRSKD+II+GGEN+SSVE+ETVLY +P V E AVVA P 
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 481 PRWGESPCAFVAVHGG---GMSEAEIIAFCRENLSHYMVPKKVVFVAELPKTSTGKIQKF 538
             WGE+PCAFV++  G     +E E+I +CR+ +  YMVPK V FV ELPKTSTGK+ KF
Sbjct: 481 VFWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKF 540