Lag0029304 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0029304
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	heavy metal-associated isoprenylated plant protein 24
Location	chr8: 37483539 .. 37484376 (-)
RNA-Seq Expression	Lag0029304
Synteny	Lag0029304

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGTTGAAGGCACTTGGGAGTACTTCTCCAATTTGATTAATAAGCACAAGAAGAAGAAGAAGCAGATGCAAACAGTGGCTTTAAAGGTTCGAATGGACTGTGAGGGCTGTGGCCGCAAGCTCAAGCAAATCATGTCTCGTGTAAAAGGTACTTAAGCTTTCGAGTTAAGTAGTGATTTTACATCGTATTGTAAACACAACCCTATATACTCCTGTATTTTCAAATCCCTTCCATCTCACTTGTCTTGTGATTGAAGTTTAATTTCTCCCCTCTTAAAATGTCAAAGTGCATAGAAACCCGTACAATATTCATGAATGTCCAGATCAACTTGCACACATCTCAATTAATCTAATGCAACAACAGTTCAACCCCAGATGTTAGAATCCTAAGCAGACAACCACCCATCGAGAGAGATTTGAATCCATTTTCTCTAAATCTCTCTACTTTTTAACTGATCCACTACGGCAACTCAAGGTGGTTTATAAAAGTCGAAACCCCGAATGATAAATTCATTGGTTTTCGTGGGGGTGTCGTGCAGGGGCGAAGCAAGTGGATGTGGATGTGAAGCAGATGAAGGTGACAGTGACAGGCTACATAGAGCCAAAGAAAGTGCTGAAGGCAGCACAAGCAACGAAGAAGAAGGTGGAGATGTGGCCGTACGTTCCAGTTGCATTGGAGCCATATCCTTATGTGTCTGCTTCGTACGACAAGAAAGCACCTCCAAACATGGTTAGAAGTGTTCCCAACACAGTCACCATTTCAGAGACTGTCATGGATGAAAACTATGTTCGAATGTTCAGTGATGACAATCCCTACGCTTGCTCTATCATGTAG

mRNA sequence

ATGGGAGTTGAAGGCACTTGGGAGTACTTCTCCAATTTGATTAATAAGCACAAGAAGAAGAAGAAGCAGATGCAAACAGTGGCTTTAAAGGTTCGAATGGACTGTGAGGGCTGTGGCCGCAAGCTCAAGCAAATCATGTCTCGTGTAAAAGGGGCGAAGCAAGTGGATGTGGATGTGAAGCAGATGAAGGTGACAGTGACAGGCTACATAGAGCCAAAGAAAGTGCTGAAGGCAGCACAAGCAACGAAGAAGAAGGTGGAGATGTGGCCGTACGTTCCAGTTGCATTGGAGCCATATCCTTATGTGTCTGCTTCGTACGACAAGAAAGCACCTCCAAACATGGTTAGAAGTGTTCCCAACACAGTCACCATTTCAGAGACTGTCATGGATGAAAACTATGTTCGAATGTTCAGTGATGACAATCCCTACGCTTGCTCTATCATGTAG

Coding sequence (CDS)

ATGGGAGTTGAAGGCACTTGGGAGTACTTCTCCAATTTGATTAATAAGCACAAGAAGAAGAAGAAGCAGATGCAAACAGTGGCTTTAAAGGTTCGAATGGACTGTGAGGGCTGTGGCCGCAAGCTCAAGCAAATCATGTCTCGTGTAAAAGGGGCGAAGCAAGTGGATGTGGATGTGAAGCAGATGAAGGTGACAGTGACAGGCTACATAGAGCCAAAGAAAGTGCTGAAGGCAGCACAAGCAACGAAGAAGAAGGTGGAGATGTGGCCGTACGTTCCAGTTGCATTGGAGCCATATCCTTATGTGTCTGCTTCGTACGACAAGAAAGCACCTCCAAACATGGTTAGAAGTGTTCCCAACACAGTCACCATTTCAGAGACTGTCATGGATGAAAACTATGTTCGAATGTTCAGTGATGACAATCCCTACGCTTGCTCTATCATGTAG

Protein sequence

MGVEGTWEYFSNLINKHKKKKKQMQTVALKVRMDCEGCGRKLKQIMSRVKGAKQVDVDVKQMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVALEPYPYVSASYDKKAPPNMVRSVPNTVTISETVMDENYVRMFSDDNPYACSIM

Homology

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BLAST of Lag0029304 vs. NCBI nr
Match: XP_022141582.1 (heavy metal-associated isoprenylated plant protein 24 [Momordica charantia])

HSP 1 Score: 285.8 bits (730), Expect = 2.1e-73
Identity = 139/148 (93.92%), Postives = 145/148 (97.97%), Query Frame = 0


Query: 1   MGVEGTWEYFSNLINKHKKKKKQMQTVALKVRMDCEGCGRKLKQIMSRVKGAKQVDVDVK 60
           MGVEGTWEYFSNLINKHKKKKKQMQTVALKVRMDCEGCGRK+KQIMSRVKGAKQVDVDVK
Sbjct: 1   MGVEGTWEYFSNLINKHKKKKKQMQTVALKVRMDCEGCGRKMKQIMSRVKGAKQVDVDVK 60

Query: 61  QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVALEPYPYVSASYDKKAPPNMVRSVPN 120
           QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPV++EPYPYVS +YDKKAPPNMVRSVPN
Sbjct: 61  QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVSIEPYPYVSVAYDKKAPPNMVRSVPN 120

Query: 121 TVTISETVMDENYVRMFSDDNPYACSIM 149
           T  I+ETV+DENYVRMFSDDNPYACSIM
Sbjct: 121 TANITETVVDENYVRMFSDDNPYACSIM 148

[Close]

BLAST of Lag0029304 vs. NCBI nr
Match: XP_008460493.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 22 [Cucumis melo] >XP_038906793.1 heavy metal-associated isoprenylated plant protein 24 [Benincasa hispida] >KAA0062412.1 heavy metal-associated isoprenylated plant protein 22 [Cucumis melo var. makuwa] >TYK26569.1 heavy metal-associated isoprenylated plant protein 22 [Cucumis melo var. makuwa])

HSP 1 Score: 283.9 bits (725), Expect = 8.1e-73
Identity = 137/148 (92.57%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 1   MGVEGTWEYFSNLINKHKKKKKQMQTVALKVRMDCEGCGRKLKQIMSRVKGAKQVDVDVK 60
           MGVEGTWEYFSNLINKH+KKKKQMQTV+LKVRMDCEGCGRK+KQIMSRVKGAK+VDVDVK
Sbjct: 1   MGVEGTWEYFSNLINKHRKKKKQMQTVSLKVRMDCEGCGRKMKQIMSRVKGAKKVDVDVK 60

Query: 61  QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVALEPYPYVSASYDKKAPPNMVRSVPN 120
           QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPV+LEPYPY+SASYDKKAPPNMVRSVPN
Sbjct: 61  QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVSLEPYPYISASYDKKAPPNMVRSVPN 120

Query: 121 TVTISETVMDENYVRMFSDDNPYACSIM 149
           T TI+ET+++ENYVRMFSDDNPYACSIM
Sbjct: 121 TATITETLVNENYVRMFSDDNPYACSIM 148

[Close]

BLAST of Lag0029304 vs. NCBI nr
Match: XP_004140315.1 (heavy metal-associated isoprenylated plant protein 24 [Cucumis sativus] >KGN51014.1 hypothetical protein Csa_008018 [Cucumis sativus])

HSP 1 Score: 283.5 bits (724), Expect = 1.1e-72
Identity = 136/148 (91.89%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 1   MGVEGTWEYFSNLINKHKKKKKQMQTVALKVRMDCEGCGRKLKQIMSRVKGAKQVDVDVK 60
           MGVEGTWEYFSNL+NKH+KKKKQMQTV+LKVRMDCEGCGRK+KQIMSRVKGAK+VDVDVK
Sbjct: 1   MGVEGTWEYFSNLVNKHRKKKKQMQTVSLKVRMDCEGCGRKMKQIMSRVKGAKKVDVDVK 60

Query: 61  QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVALEPYPYVSASYDKKAPPNMVRSVPN 120
           QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPV+LEPYPY+SASYDKKAPPNMVRSVPN
Sbjct: 61  QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVSLEPYPYISASYDKKAPPNMVRSVPN 120

Query: 121 TVTISETVMDENYVRMFSDDNPYACSIM 149
           T TI+ET+++ENYVRMFSDDNPYACSIM
Sbjct: 121 TATITETLVNENYVRMFSDDNPYACSIM 148

[Close]

BLAST of Lag0029304 vs. NCBI nr
Match: XP_022939381.1 (heavy metal-associated isoprenylated plant protein 24 [Cucurbita moschata] >XP_022992863.1 heavy metal-associated isoprenylated plant protein 24 [Cucurbita maxima] >XP_023550057.1 heavy metal-associated isoprenylated plant protein 24 [Cucurbita pepo subsp. pepo] >KAG6579015.1 Heavy metal-associated isoprenylated plant protein 24, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016538.1 Heavy metal-associated isoprenylated plant protein 24, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 282.3 bits (721), Expect = 2.3e-72
Identity = 137/148 (92.57%), Postives = 146/148 (98.65%), Query Frame = 0


Query: 1   MGVEGTWEYFSNLINKHKKKKKQMQTVALKVRMDCEGCGRKLKQIMSRVKGAKQVDVDVK 60
           MGVEGTWEYFSNLI KH+KKKKQMQTV+LKVRMDCEGCGRK+KQIMSRVKGAKQVDVDVK
Sbjct: 1   MGVEGTWEYFSNLIYKHRKKKKQMQTVSLKVRMDCEGCGRKMKQIMSRVKGAKQVDVDVK 60

Query: 61  QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVALEPYPYVSASYDKKAPPNMVRSVPN 120
           QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPV+LEPYPY+SASYDKKAPPNMVRSVPN
Sbjct: 61  QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVSLEPYPYISASYDKKAPPNMVRSVPN 120

Query: 121 TVTISETVMDENYVRMFSDDNPYACSIM 149
           T TI+ET+++ENYVRMFSDDNPYACSIM
Sbjct: 121 TATITETLVNENYVRMFSDDNPYACSIM 148

[Close]

BLAST of Lag0029304 vs. NCBI nr
Match: XP_023876385.1 (heavy metal-associated isoprenylated plant protein 24 [Quercus suber])

HSP 1 Score: 225.3 bits (573), Expect = 3.4e-55
Identity = 113/150 (75.33%), Postives = 129/150 (86.00%), Query Frame = 0


Query: 1   MGVEGTWEYFSNLIN--KHKKKKKQMQTVALKVRMDCEGCGRKLKQIMSRVKGAKQVDVD 60
           MGVEGT EY S L++  K +KKKKQMQTVALKVRMDCEGC RK+K ++S VKGAK VDVD
Sbjct: 1   MGVEGTLEYLSALLSSAKKRKKKKQMQTVALKVRMDCEGCERKVKNVLSGVKGAKSVDVD 60

Query: 61  VKQMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVALEPYPYVSASYDKKAPPNMVRSV 120
            KQ KVTVTGY+EPKKVLKAAQ+TKKKVEMWPYVP AL  +PYV+ +YDKKAPPNMVRS+
Sbjct: 61  RKQQKVTVTGYVEPKKVLKAAQSTKKKVEMWPYVPYALVAHPYVAQAYDKKAPPNMVRSI 120

Query: 121 PNTVTISETVMDENYVRMFSDDNPYACSIM 149
           PNTVTI+ET +D+ Y  MFSD+NP ACSIM
Sbjct: 121 PNTVTINETTVDDRYTTMFSDENPNACSIM 150

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BLAST of Lag0029304 vs. ExPASy Swiss-Prot
Match: O81464 (Heavy metal-associated isoprenylated plant protein 24 OS=Arabidopsis thaliana OX=3702 GN=HIPP24 PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 3.1e-51
Identity = 100/150 (66.67%), Postives = 125/150 (83.33%), Query Frame = 0


Query: 1   MGVEGTWEYFSNLINKHK-KKKKQMQTVALKV-RMDCEGCGRKLKQIMSRVKGAKQVDVD 60
           MGVEGT EY S+L+ K K KKKKQMQTVAL+V R+DCEGC RK+K ++S VKG K VDVD
Sbjct: 1   MGVEGTMEYISDLLKKRKRKKKKQMQTVALRVARIDCEGCERKIKHVLSGVKGVKSVDVD 60

Query: 61  VKQMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVALEPYPYVSASYDKKAPPNMVRSV 120
           VK  KVTVTGYI+PKKVL+AA++TKKKVE+WPYVP  +   PY+S +YDKKAPPNMVR V
Sbjct: 61  VKLQKVTVTGYIDPKKVLEAAKSTKKKVELWPYVPYTMVANPYISQAYDKKAPPNMVRKV 120

Query: 121 PNTVTISETVMDENYVRMFSDDNPYACSIM 149
           P+T +++ET +D++Y  MFSD+NP +C+IM
Sbjct: 121 PDTASVNETTVDDSYTIMFSDENPNSCAIM 150

[Close]

BLAST of Lag0029304 vs. ExPASy Swiss-Prot
Match: O65657 (Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX=3702 GN=HIPP23 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 1.9e-37
Identity = 87/158 (55.06%), Postives = 105/158 (66.46%), Query Frame = 0


Query: 1   MGVEGTWEYFSNLINK-------HKKKKKQMQTVALKVRMDCEGCGRKLKQIMSRVKGAK 60
           MGV GT EY S LI          +KKKKQ QTV LKVRMDC+GC  K+K  +S +KG K
Sbjct: 1   MGVGGTLEYISELIGNGGSHSYGKRKKKKQFQTVELKVRMDCDGCVLKIKNSLSSLKGVK 60

Query: 61  QVDVDVKQMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVALEPYPYVSASYDKKAPPN 120
            V+++ KQ KVTV+GY +  KVLK A+AT KK E+WPYVP  L   PY++ +YDKKAPP 
Sbjct: 61  TVEINKKQQKVTVSGYADASKVLKKAKATGKKAEIWPYVPYNLVAQPYIAQAYDKKAPPG 120

Query: 121 MVRSV-PNTVTISETVM--DENYVRMFSDDNPYACSIM 149
            VR V PN  T +  V   D +Y  +FSDDNP ACSIM
Sbjct: 121 YVRKVDPNVTTGTMAVYYDDPSYTSLFSDDNPNACSIM 158

[Close]

BLAST of Lag0029304 vs. ExPASy Swiss-Prot
Match: Q93VP2 (Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana OX=3702 GN=HIPP22 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 8.5e-33
Identity = 72/145 (49.66%), Postives = 100/145 (68.97%), Query Frame = 0


Query: 8   EYFSN---LINKHKKKKKQMQTVALKVRMDCEGCGRKLKQIMSRVKGAKQVDVDVKQMKV 67
           EYFSN   +  + +KK+K MQTV +KV++DC+GC RK+K  +S +KGAK V+V+ K  KV
Sbjct: 9   EYFSNHFYVSIRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVEVNRKMHKV 68

Query: 68  TVTGYIEPKKVLKAAQAT-KKKVEMWPYVPVALEPYPYVSASYDKKAPPNMVRSVPNTVT 127
           TV+GY++PKKVLK  Q+T KKK E+WPYVP  +  YPY + +YDK+APP  VR       
Sbjct: 69  TVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFVRK-SEQAQ 128

Query: 128 ISETVMDENYVRMFSDDNPYACSIM 149
                 D+  + +FSD+NP AC++M
Sbjct: 129 AQPGSTDDKLMSLFSDENPNACTVM 152

[Close]

BLAST of Lag0029304 vs. ExPASy Swiss-Prot
Match: Q9C9A3 (Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana OX=3702 GN=HIPP20 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 6.1e-31
Identity = 68/144 (47.22%), Postives = 99/144 (68.75%), Query Frame = 0


Query: 8   EYFSNL--INKHKKKKKQMQTVALKVRMDCEGCGRKLKQIMSRVKGAKQVDVDVKQMKVT 67
           EY S+   + + ++K+K MQTV +KV+MDC+GC R++K  +S +KG K V+V+ K  KVT
Sbjct: 9   EYISDYFRVTRKRRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEVNRKIHKVT 68

Query: 68  VTGYIEPKKVLKAAQATKKKVEMWPYVPVALEPYPYVSASYDKKAPPNMVR-SVPNTVTI 127
           V+GY+EPKKVLK  + T KK E+WPYVP  +  YPY   +YDKKAP   VR S  + + +
Sbjct: 69  VSGYVEPKKVLKRIERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVRKSEQSQLQL 128

Query: 128 SETVMDENYVRMFSDDNPYACSIM 149
                + +Y+ +FSD+NP AC++M
Sbjct: 129 LPGAPENHYISLFSDENPNACTVM 152

[Close]

BLAST of Lag0029304 vs. ExPASy Swiss-Prot
Match: Q9LF57 (Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana OX=3702 GN=HIPP21 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-28
Identity = 64/149 (42.95%), Postives = 98/149 (65.77%), Query Frame = 0


Query: 5   GTWEYFSNLIN----KHKKKKKQMQTVALKVRMDCEGCGRKLKQIMSRVKGAKQVDVDVK 64
           G ++Y S+  +      K K+K +QTV +KV+MDC+GC R+++ ++ R+KG K V+V+ K
Sbjct: 2   GAFDYISSFCSYTYANAKTKRKPLQTVDIKVKMDCDGCERRVRNVVRRMKGVKSVEVNRK 61

Query: 65  QMKVTVTGYIEPKKVLKAAQATKKKVEMWPYVPVALEPYPYVSASYDKKAPPNMVRSVPN 124
           Q ++TV G+++P KVLK  ++T KK E WPY+P  +  YP+    YDK+AP   +R+ P 
Sbjct: 62  QSRITVNGHVDPNKVLKRVKSTGKKAEFWPYIPQHMVYYPFAPGMYDKRAPAGHIRN-PT 121

Query: 125 TVTISETVMDENYVRMFSDDNPY-ACSIM 149
               +    +ENYV +FSDDN + ACSIM
Sbjct: 122 QSFPTANAPEENYVSLFSDDNVHAACSIM 149

The following BLAST results are available for this feature:

BLAST of Lag0029304 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141582.1	2.1e-73	93.92	heavy metal-associated isoprenylated plant protein 24 [Momordica charantia]	[more]
XP_008460493.1	8.1e-73	92.57	PREDICTED: heavy metal-associated isoprenylated plant protein 22 [Cucumis melo] ...	[more]
XP_004140315.1	1.1e-72	91.89	heavy metal-associated isoprenylated plant protein 24 [Cucumis sativus] >KGN5101...	[more]
XP_022939381.1	2.3e-72	92.57	heavy metal-associated isoprenylated plant protein 24 [Cucurbita moschata] >XP_0...	[more]
XP_023876385.1	3.4e-55	75.33	heavy metal-associated isoprenylated plant protein 24 [Quercus suber]	[more]

BLAST of Lag0029304 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81464	3.1e-51	66.67	Heavy metal-associated isoprenylated plant protein 24 OS=Arabidopsis thaliana OX...	[more]
O65657	1.9e-37	55.06	Heavy metal-associated isoprenylated plant protein 23 OS=Arabidopsis thaliana OX...	[more]
Q93VP2	8.5e-33	49.66	Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana OX...	[more]
Q9C9A3	6.1e-31	47.22	Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana OX...	[more]
Q9LF57	1.3e-28	42.95	Heavy metal-associated isoprenylated plant protein 21 OS=Arabidopsis thaliana OX...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 30..84 e-value: 1.1E-8 score: 35.4
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 33..88 score: 9.64562
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 33..87 e-value: 1.18191E-9 score: 49.5265
None	No IPR available	GENE3D	3.30.70.100		coord: 11..90 e-value: 4.8E-15 score: 57.5
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 3..148
None	No IPR available	PANTHER	PTHR22814:SF84	HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 24	coord: 3..148
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 23..90

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0029304.1	Lag0029304.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding