Lag0027196 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0027196
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Dynein light chain
Location	chr10: 45758436 .. 45760344 (+)
RNA-Seq Expression	Lag0027196
Synteny	Lag0027196

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCTTTATGTCTCCGGGAGTCAAGAAAAGTATTCATAGGAAAATAAAAGGTGCCACACTCTAATCGCCTGTAATCTCAATTCAGTAAGGCCATCCTCTTGAATCTATTGGGGAACAGAACACGTTTTTCTTCCAGCGCCCCCCTCCCTCCTTCCCCCAATCCCATAGTGTAACTTATGCTTCGACATCTACTGTAATATCTACTCAGAACTACTGTAAAATTAATATCCTGCAGCAATAAAAGGTTAACAAAATTCATGAATAGCCAACTTATTTGGTTCATTGCACTCTTTGTTATTAACATGCTGACATCAAGGCTTTGATCATCAAAACATCTGCTTGAAACACTTATGCTGAGAGATTTCCACCCACAAGTTTTAAGATTCTAATCTTCCATATATTTGTCTAAAGTATATACCTTCAACATTCTTATTTTAGATGTTTGGCCTTTCCCTGTGTATTCAATTATAGCTGTGAGTTGTGAGGTGCAACTCCCAACACAAACGCGAACCCAAGCAAAACAAACATCCCTTTCTGTGATTTCTGAGGCCACCAATCAAAACGTCCAAGAATTAAATCCCAACTTTGAAATAATCTTAGATTAGTATTGGTGTATCATACAAAAAGTATGTGTTTCTCAGAAGATATTGTTGTGTCACAACTGTATTGATTTAACTCCTGCTAATGCAAACAAAGTTTGCTAATTTTGTTGTTTTAGAGGTAAAAGAAATGGGGTTTTCCTGAGTAGAGCAGAGAGCAACCACGGAAATATCATCATTTGGCTTTGAAGCACAGCCACCAGATTACCCGAGTATCCTCCTTTTATCAAAAATACTCTTCACTTCTCTGACATTGGGCTATAAATTTGCAGAGGTTCGTGCTAGCAGGCTTTTGCAGGCTGGTTGGTACAGAAAAGCCAACCTGCCATCGCTTTTGAAGATGTTGGAGGGCAAAGCTGTGGTCCGCGAAACAGACATGCCAGAGGGGATCCAGGGCCATGTCCTTGGGTTGGCTTATAAAGCTCTTGATCAACACGAAGTTTCCGACTGTCAATCCATTGCACGTTTCATCAAACAGGTTTATTTTCTTAAACACTCAGAATCTTCTTTATGTAGTGGTTTAAATTTCAATTTTATTAAATTTAAAATATCCCCTTTTTCCCTTTTATTTCAAAAAAGGAAGACAAGTCCTTTCATTGCAATAATAGATTGAAAAGATTCATTTTTGTAACCTGTAAAAGTTGAGGAGACCCTTTTATGATATAAAGAAGATATGCTTCATCATTCACAGTAACCACATCAATAAACAAAGTTACCTTTTCCTTTTAAAATATTCGAGTGTGTGTCATTTTCAGATACTATAAATTTCTACACAAAAATATAGCTGATCTATGTACACGTCATCCACCATCTGATCTGATATGATGAGACTAAATCTGGAATATACTTGTGTGGATGATGTTTGAATCAACTTGTCTGAAGAGTAAAGGGACATCATTTTGAATGAAATGACCCAGAAGTTCCCATGTTCATTATTTCTATCTTTTTGCTGGTTTGTTGATCGAACAATCTCAGTATAATAATTAATAATGAGAAAAAGCTGCTAGATATCAAATTGTTTAGTATCGAATGGACCAACCCGTGACATATTAATGAAAGATTACAAAATAATGTCTGTAAGAACTGCATACTGATATGACTTTGAGCTTTGATAATTAGAGGTTTGATGAAGCTTATGGCCCGGCTTGGCATTGTGTGGTGGGCAGAGCGTTTGGAGGTTGCATTACGCATTTATCTGGTAGCTTCATCTTCTTCCATGTGGATACCATGGAGTTTCTCATCTTTAAGGACGGAAGAGACTGCTCCAAAAGCATTGAAGAGACCGTCGGAGTTCGCCAGAAAAGTTAA

mRNA sequence

ATGTCCTTTATGTCTCCGGGAGTCAAGAAAAGTATTCATAGGAAAATAAAAGAGGTTCGTGCTAGCAGGCTTTTGCAGGCTGGTTGGTACAGAAAAGCCAACCTGCCATCGCTTTTGAAGATGTTGGAGGGCAAAGCTGTGGTCCGCGAAACAGACATGCCAGAGGGGATCCAGGGCCATGTCCTTGGGTTGGCTTATAAAGCTCTTGATCAACACGAAGTTTCCGACTGTCAATCCATTGCACGTTTCATCAAACAGAGGTTTGATGAAGCTTATGGCCCGGCTTGGCATTGTGTGGTGGGCAGAGCGTTTGGAGGTTGCATTACGCATTTATCTGGTAGCTTCATCTTCTTCCATGTGGATACCATGGAGTTTCTCATCTTTAAGGACGGAAGAGACTGCTCCAAAAGCATTGAAGAGACCGTCGGAGTTCGCCAGAAAAGTTAA

Coding sequence (CDS)

ATGTCCTTTATGTCTCCGGGAGTCAAGAAAAGTATTCATAGGAAAATAAAAGAGGTTCGTGCTAGCAGGCTTTTGCAGGCTGGTTGGTACAGAAAAGCCAACCTGCCATCGCTTTTGAAGATGTTGGAGGGCAAAGCTGTGGTCCGCGAAACAGACATGCCAGAGGGGATCCAGGGCCATGTCCTTGGGTTGGCTTATAAAGCTCTTGATCAACACGAAGTTTCCGACTGTCAATCCATTGCACGTTTCATCAAACAGAGGTTTGATGAAGCTTATGGCCCGGCTTGGCATTGTGTGGTGGGCAGAGCGTTTGGAGGTTGCATTACGCATTTATCTGGTAGCTTCATCTTCTTCCATGTGGATACCATGGAGTTTCTCATCTTTAAGGACGGAAGAGACTGCTCCAAAAGCATTGAAGAGACCGTCGGAGTTCGCCAGAAAAGTTAA

Protein sequence

MSFMSPGVKKSIHRKIKEVRASRLLQAGWYRKANLPSLLKMLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWHCVVGRAFGGCITHLSGSFIFFHVDTMEFLIFKDGRDCSKSIEETVGVRQKS

Homology

[Close]

BLAST of Lag0027196 vs. NCBI nr
Match: XP_022145583.1 (dynein light chain-like [Momordica charantia])

HSP 1 Score: 208.8 bits (530), Expect = 3.3e-50
Identity = 99/108 (91.67%), Postives = 101/108 (93.52%), Query Frame = 0


Query: 41  MLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWHCVV 100
           MLEGKA VRETDMPEGIQ HVL LAYKALD HEVSDCQSIARFIKQRFDEAYGPAWHCVV
Sbjct: 1   MLEGKAAVRETDMPEGIQSHVLELAYKALDHHEVSDCQSIARFIKQRFDEAYGPAWHCVV 60

Query: 101 GRAFGGCITHLSGSFIFFHVDTMEFLIFKDGRDCSKSIEETVGVRQKS 149
           G+AFGGCITHL GSFIFFHVD MEFLIFKDGRD SKSIEET+GVRQKS
Sbjct: 61  GKAFGGCITHLCGSFIFFHVDIMEFLIFKDGRDFSKSIEETLGVRQKS 108

[Close]

BLAST of Lag0027196 vs. NCBI nr
Match: XP_008442502.1 (PREDICTED: dynein light chain 1, cytoplasmic-like [Cucumis melo])

HSP 1 Score: 208.8 bits (530), Expect = 3.3e-50
Identity = 96/107 (89.72%), Postives = 101/107 (94.39%), Query Frame = 0


Query: 41  MLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWHCVV 100
           MLEGKAVVRETDMP+GIQ HVL LAYKALDQHEVSDCQSIA FIKQRFDEAYGPAWHCV 
Sbjct: 1   MLEGKAVVRETDMPQGIQSHVLELAYKALDQHEVSDCQSIAHFIKQRFDEAYGPAWHCVA 60

Query: 101 GRAFGGCITHLSGSFIFFHVDTMEFLIFKDGRDCSKSIEETVGVRQK 148
           G+AFGGCITHL GSFIFFHVDTMEFL+FKDGRDCSKS+EETVGV +K
Sbjct: 61  GKAFGGCITHLCGSFIFFHVDTMEFLVFKDGRDCSKSVEETVGVCRK 107

[Close]

BLAST of Lag0027196 vs. NCBI nr
Match: KGN58830.1 (hypothetical protein Csa_001625 [Cucumis sativus])

HSP 1 Score: 205.3 bits (521), Expect = 3.6e-49
Identity = 94/107 (87.85%), Postives = 100/107 (93.46%), Query Frame = 0


Query: 41  MLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWHCVV 100
           MLEGKAVVRETDMP+GIQ HVL LAYKALDQHEVSDCQSIA FIKQRFDEAYGPAWHCV 
Sbjct: 1   MLEGKAVVRETDMPQGIQSHVLELAYKALDQHEVSDCQSIAHFIKQRFDEAYGPAWHCVA 60

Query: 101 GRAFGGCITHLSGSFIFFHVDTMEFLIFKDGRDCSKSIEETVGVRQK 148
           G+AFGGCITHL GS+IFFHVDTMEFL+FKDGRDC KS+EETVGV +K
Sbjct: 61  GKAFGGCITHLCGSYIFFHVDTMEFLVFKDGRDCFKSVEETVGVCRK 107

[Close]

BLAST of Lag0027196 vs. NCBI nr
Match: KAG7027933.1 (putative receptor protein kinase ZmPK1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 181.4 bits (459), Expect = 5.6e-42
Identity = 85/106 (80.19%), Postives = 93/106 (87.74%), Query Frame = 0


Query: 38  LLKMLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWH 97
           +LKMLEGKAVVRETDM E IQGH L LAYKALDQHEVSD QSIARFIKQR DEAYGPAW 
Sbjct: 805 VLKMLEGKAVVRETDMAEVIQGHGLALAYKALDQHEVSDSQSIARFIKQRLDEAYGPAWQ 864

Query: 98  CVVGRAFGGCITHLSGSFIFFHVDTMEFLIFKDGRDCSKSIEETVG 144
           CVVG+AFGGCITH+ G+FI FHVDTMEFLIFKD  DC K+++ T+G
Sbjct: 865 CVVGKAFGGCITHVCGTFISFHVDTMEFLIFKDATDCFKTVQHTLG 910

[Close]

BLAST of Lag0027196 vs. NCBI nr
Match: XP_023540066.1 (dynein light chain-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 180.3 bits (456), Expect = 1.3e-41
Identity = 84/103 (81.55%), Postives = 90/103 (87.38%), Query Frame = 0


Query: 41  MLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWHCVV 100
           MLEGK VVRETDM E IQGH L LAYKALDQHEVSD QSIARFIKQR DEAYGPAW CVV
Sbjct: 1   MLEGKGVVRETDMAEVIQGHGLALAYKALDQHEVSDSQSIARFIKQRLDEAYGPAWQCVV 60

Query: 101 GRAFGGCITHLSGSFIFFHVDTMEFLIFKDGRDCSKSIEETVG 144
           G+AFGGCITH+ G+FIFFHVDTMEFLIFKD  DC KS++ T+G
Sbjct: 61  GKAFGGCITHVCGTFIFFHVDTMEFLIFKDATDCFKSLQHTLG 103

[Close]

BLAST of Lag0027196 vs. ExPASy Swiss-Prot
Match: Q94758 (Dynein light chain OS=Schistosoma mansoni OX=6183 GN=DLC PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.5e-16
Identity = 40/91 (43.96%), Postives = 59/91 (64.84%), Query Frame = 0


Query: 41  MLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWHCVV 100
           M E KAV++  DM E +Q   + +A  A+D+H++   + IA  IK+ FD  Y P WHC+V
Sbjct: 1   MSERKAVIKNADMSEEMQEDAIHIAAGAIDKHDLE--KDIAANIKKDFDRKYHPTWHCIV 60

Query: 101 GRAFGGCITHLSGSFIFFHVDTMEFLIFKDG 132
           GR FG  +TH + +FI+F++D   FL+FK G
Sbjct: 61  GRHFGSYVTHETHNFIYFYLDDRAFLLFKSG 89

[Close]

BLAST of Lag0027196 vs. ExPASy Swiss-Prot
Match: O02414 (Dynein light chain LC6, flagellar outer arm OS=Heliocidaris crassispina OX=1043166 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.5e-16
Identity = 38/91 (41.76%), Postives = 58/91 (63.74%), Query Frame = 0


Query: 41  MLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWHCVV 100
           M E KAV++  DMPE +Q   +  A +AL++  +   + IA +IK+ FD+ Y P WHC+V
Sbjct: 1   MSERKAVIKNADMPEDMQQDAVDCATQALEKFNIE--KDIAAYIKKEFDKKYNPTWHCIV 60

Query: 101 GRAFGGCITHLSGSFIFFHVDTMEFLIFKDG 132
           GR FG  +TH +  FI+F++  +  L+FK G
Sbjct: 61  GRNFGSYVTHETKHFIYFYLGQVAVLLFKSG 89

[Close]

BLAST of Lag0027196 vs. ExPASy Swiss-Prot
Match: Q94748 (Probable dynein light chain OS=Schistosoma mansoni OX=6183 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.6e-15
Identity = 38/91 (41.76%), Postives = 57/91 (62.64%), Query Frame = 0


Query: 41  MLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWHCVV 100
           M E KAV++  DM E +Q   +  A  ALD++E+   + +A +IK+ FD  Y P WHC+V
Sbjct: 1   MGERKAVIKNADMHEDMQETAVHTAAAALDKYEIE--KDVAAYIKKEFDRKYNPNWHCIV 60

Query: 101 GRAFGGCITHLSGSFIFFHVDTMEFLIFKDG 132
           G+ FG  +TH +  FI+F++    FL+FK G
Sbjct: 61  GKHFGSYVTHETQHFIYFYLQERAFLLFKSG 89

[Close]

BLAST of Lag0027196 vs. ExPASy Swiss-Prot
Match: O96860 (Dynein light chain 2, cytoplasmic OS=Drosophila melanogaster OX=7227 GN=Cdlc2 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.1e-15
Identity = 37/91 (40.66%), Postives = 58/91 (63.74%), Query Frame = 0


Query: 41  MLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWHCVV 100
           M + KAV++  DM E +Q   +  A +AL+++ +   + IA FIK+ FD+ Y P WHC+V
Sbjct: 1   MSDRKAVIKNADMSEEMQQDAVDCATQALEKYNIE--KDIAAFIKKEFDKKYNPTWHCIV 60

Query: 101 GRAFGGCITHLSGSFIFFHVDTMEFLIFKDG 132
           GR FG  +TH +  FI+F++  +  L+FK G
Sbjct: 61  GRNFGSYVTHETRHFIYFYLGQVAILLFKSG 89

[Close]

BLAST of Lag0027196 vs. ExPASy Swiss-Prot
Match: Q22799 (Dynein light chain 1, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=dlc-1 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-15
Identity = 35/91 (38.46%), Postives = 59/91 (64.84%), Query Frame = 0


Query: 41  MLEGKAVVRETDMPEGIQGHVLGLAYKALDQHEVSDCQSIARFIKQRFDEAYGPAWHCVV 100
           M++ KAV++  DM + +Q   +  A +AL+++ +   + IA +IK+ FD+ Y P WHC+V
Sbjct: 1   MVDRKAVIKNADMSDDMQQDAIDCATQALEKYNIE--KDIAAYIKKEFDKKYNPTWHCIV 60

Query: 101 GRAFGGCITHLSGSFIFFHVDTMEFLIFKDG 132
           GR FG  +TH +  FI+F++  +  L+FK G
Sbjct: 61  GRNFGSYVTHETKHFIYFYLGQVAILLFKSG 89

The following BLAST results are available for this feature:

BLAST of Lag0027196 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145583.1	3.3e-50	91.67	dynein light chain-like [Momordica charantia]	[more]
XP_008442502.1	3.3e-50	89.72	PREDICTED: dynein light chain 1, cytoplasmic-like [Cucumis melo]	[more]
KGN58830.1	3.6e-49	87.85	hypothetical protein Csa_001625 [Cucumis sativus]	[more]
KAG7027933.1	5.6e-42	80.19	putative receptor protein kinase ZmPK1, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_023540066.1	1.3e-41	81.55	dynein light chain-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0027196 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94758	1.5e-16	43.96	Dynein light chain OS=Schistosoma mansoni OX=6183 GN=DLC PE=1 SV=1	[more]
O02414	5.5e-16	41.76	Dynein light chain LC6, flagellar outer arm OS=Heliocidaris crassispina OX=10431...	[more]
Q94748	1.6e-15	41.76	Probable dynein light chain OS=Schistosoma mansoni OX=6183 PE=3 SV=1	[more]
O96860	2.1e-15	40.66	Dynein light chain 2, cytoplasmic OS=Drosophila melanogaster OX=7227 GN=Cdlc2 PE...	[more]
Q22799	2.7e-15	38.46	Dynein light chain 1, cytoplasmic OS=Caenorhabditis elegans OX=6239 GN=dlc-1 PE=...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001372	Dynein light chain, type 1/2	SMART	SM01375	Dynein_light_2	coord: 41..130 e-value: 6.7E-38 score: 141.9
IPR001372	Dynein light chain, type 1/2	PFAM	PF01221	Dynein_light	coord: 45..129 e-value: 6.0E-29 score: 100.3
IPR001372	Dynein light chain, type 1/2	PANTHER	PTHR11886	DYNEIN LIGHT CHAIN	coord: 41..147
IPR037177	Dynein light chain superfamily	GENE3D	3.30.740.10	Protein Inhibitor Of Neuronal Nitric Oxide Synthase;	coord: 35..130 e-value: 2.2E-29 score: 103.3
IPR037177	Dynein light chain superfamily	SUPERFAMILY	54648	DLC	coord: 40..130
None	No IPR available	PANTHER	PTHR11886:SF85	DYNEIN LIGHT CHAIN	coord: 41..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0027196.1	Lag0027196.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007017	microtubule-based process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0030286	dynein complex
cellular_component	GO:0005874	microtubule
molecular_function	GO:0045505	dynein intermediate chain binding
molecular_function	GO:0051959	dynein light intermediate chain binding