Lag0026972 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0026972
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr10: 43858790 .. 43859719 (+)
RNA-Seq Expression	Lag0026972
Synteny	Lag0026972

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTTGAAATTGCAGGTTTTATTTTTGTCTCCATTCTGTGGGGTTTCTGGGTATGGCGCCGCCGTGGATCGGCGGCGACGGAGCAGCTTCCGCCGGGGCCACCTCGTTGGCCAGTGGTCGGAAACTTGCCCCAACTCAGCTTCTCCGCTCACGAATCCTTCACCAAACTCGCCACCAAGTACGGCCCAATCATGACCCTCCATCTCGGGTCGATGAGCACGGTGGTCGTCTCGTCGAGCACGGCGGCGCGTGAGATGTTCAAAAACCACGACGCGCCGCTGGCAGGACGCATGATTTACGAGGCAATGAAAGGGAACCACGGCACCGAAGGCTCCCTAATCACCTCCCAGTACGGGCCCCACTGGCGGATGCTGAGACGCCTCGCCACCACCGAGTTCTTCGTCACGCGCCGCCTCGACGCCATGCGACACATCCGCCGGCGCTGCGTGGACGCGATGATCCGCCACGTGGGCGACAACGGCGCGTGTGGGGACGCGCCGCCGATCGAGTTGGGGAGATTGTTCTTCATAATGAGCTTTAACCTGATCGGGAATCTGATATTTTCGAAAGATCTAGTGGAGCGGAATTCGACGAGGGGGAAGGGATTTTACTACCATGCGAGACGGGTGATGGAGATGGCCGGAAAGCCGAACGTGGCGGACTTTTTGCCGGCGTTCCGGTGGGTGGATCCGCAGGGGATAAGGAGGAAGACGCAGTTTCATGTGAAGAAAGCGTTTGAAATTGCAGGGGAGTTTATAAGAGAGAGATTGAAGGAAAAAGAAAGTGAAGAGAAGAGAAAAGAAGAAGGAAGAAAGGATTATTTGGATGTGCTTTTGGAGTTTCGTGGCGATGGCGTCGAGGAGCCTTCATGTTTCTCTTCTTGGATCATCAACGTTATCGTCTTCGTAAGCTATTATTATTCATAA

mRNA sequence

ATGGAGTTTGAAATTGCAGGTTTTATTTTTGTCTCCATTCTGTGGGGTTTCTGGGTATGGCGCCGCCGTGGATCGGCGGCGACGGAGCAGCTTCCGCCGGGGCCACCTCGTTGGCCAGTGGTCGGAAACTTGCCCCAACTCAGCTTCTCCGCTCACGAATCCTTCACCAAACTCGCCACCAAGTACGGCCCAATCATGACCCTCCATCTCGGGTCGATGAGCACGGTGGTCGTCTCGTCGAGCACGGCGGCGCGTGAGATGTTCAAAAACCACGACGCGCCGCTGGCAGGACGCATGATTTACGAGGCAATGAAAGGGAACCACGGCACCGAAGGCTCCCTAATCACCTCCCAGTACGGGCCCCACTGGCGGATGCTGAGACGCCTCGCCACCACCGAGTTCTTCGTCACGCGCCGCCTCGACGCCATGCGACACATCCGCCGGCGCTGCGTGGACGCGATGATCCGCCACGTGGGCGACAACGGCGCGTGTGGGGACGCGCCGCCGATCGAGTTGGGGAGATTGTTCTTCATAATGAGCTTTAACCTGATCGGGAATCTGATATTTTCGAAAGATCTAGTGGAGCGGAATTCGACGAGGGGGAAGGGATTTTACTACCATGCGAGACGGGTGATGGAGATGGCCGGAAAGCCGAACGTGGCGGACTTTTTGCCGGCGTTCCGGTGGGTGGATCCGCAGGGGATAAGGAGGAAGACGCAGTTTCATGTGAAGAAAGCGTTTGAAATTGCAGGGGAGTTTATAAGAGAGAGATTGAAGGAAAAAGAAAGTGAAGAGAAGAGAAAAGAAGAAGGAAGAAAGGATTATTTGGATGTGCTTTTGGAGTTTCGTGGCGATGGCGTCGAGGAGCCTTCATGTTTCTCTTCTTGGATCATCAACGTTATCGTCTTCGTAAGCTATTATTATTCATAA

Coding sequence (CDS)

ATGGAGTTTGAAATTGCAGGTTTTATTTTTGTCTCCATTCTGTGGGGTTTCTGGGTATGGCGCCGCCGTGGATCGGCGGCGACGGAGCAGCTTCCGCCGGGGCCACCTCGTTGGCCAGTGGTCGGAAACTTGCCCCAACTCAGCTTCTCCGCTCACGAATCCTTCACCAAACTCGCCACCAAGTACGGCCCAATCATGACCCTCCATCTCGGGTCGATGAGCACGGTGGTCGTCTCGTCGAGCACGGCGGCGCGTGAGATGTTCAAAAACCACGACGCGCCGCTGGCAGGACGCATGATTTACGAGGCAATGAAAGGGAACCACGGCACCGAAGGCTCCCTAATCACCTCCCAGTACGGGCCCCACTGGCGGATGCTGAGACGCCTCGCCACCACCGAGTTCTTCGTCACGCGCCGCCTCGACGCCATGCGACACATCCGCCGGCGCTGCGTGGACGCGATGATCCGCCACGTGGGCGACAACGGCGCGTGTGGGGACGCGCCGCCGATCGAGTTGGGGAGATTGTTCTTCATAATGAGCTTTAACCTGATCGGGAATCTGATATTTTCGAAAGATCTAGTGGAGCGGAATTCGACGAGGGGGAAGGGATTTTACTACCATGCGAGACGGGTGATGGAGATGGCCGGAAAGCCGAACGTGGCGGACTTTTTGCCGGCGTTCCGGTGGGTGGATCCGCAGGGGATAAGGAGGAAGACGCAGTTTCATGTGAAGAAAGCGTTTGAAATTGCAGGGGAGTTTATAAGAGAGAGATTGAAGGAAAAAGAAAGTGAAGAGAAGAGAAAAGAAGAAGGAAGAAAGGATTATTTGGATGTGCTTTTGGAGTTTCGTGGCGATGGCGTCGAGGAGCCTTCATGTTTCTCTTCTTGGATCATCAACGTTATCGTCTTCGTAAGCTATTATTATTCATAA

Protein sequence

MEFEIAGFIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDNGACGDAPPIELGRLFFIMSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFRGDGVEEPSCFSSWIINVIVFVSYYYS

Homology

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BLAST of Lag0026972 vs. NCBI nr
Match: XP_038905939.1 (iridoid oxidase [Benincasa hispida])

HSP 1 Score: 490.0 bits (1260), Expect = 1.5e-134
Identity = 239/309 (77.35%), Postives = 265/309 (85.76%), Query Frame = 0


Query: 1   MEFEIAGFIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLAT 60
           ME +IAG I +  LWG WVWR RG+ A EQLPPGPPRWPVVGNL QLSFSAHESFTKLA 
Sbjct: 1   MEIQIAGLILLLFLWGLWVWRHRGAVADEQLPPGPPRWPVVGNLLQLSFSAHESFTKLAA 60

Query: 61  KYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYG 120
           KYGPIMTL LGSM TVVVSSS AAREMFK HDAPL+GRMIYEAMKG HGTEGSLITSQYG
Sbjct: 61  KYGPIMTLQLGSMKTVVVSSSAAAREMFKTHDAPLSGRMIYEAMKGEHGTEGSLITSQYG 120

Query: 121 PHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDNGACG-DAPPIELGRLFFIM 180
           PHWRMLRRLAT+EFFVTR+L+AM+H+RRRC+D MIRH+ +    G  APP+++G+  FIM
Sbjct: 121 PHWRMLRRLATSEFFVTRQLEAMKHVRRRCIDTMIRHIEEEIEGGVHAPPLDVGKFMFIM 180

Query: 181 SFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKT 240
           SFNLIGNLIFSKDL+E NS RGK FYYH R+VMEMAGKPNVADFLPA RW+DPQGIR+KT
Sbjct: 181 SFNLIGNLIFSKDLLEANSKRGKEFYYHTRKVMEMAGKPNVADFLPALRWMDPQGIRKKT 240

Query: 241 QFHVKKAFEIAGEFIRERLKEKES-----EEKRKEEGRKDYLDVLLEFRGDGVEEPSCFS 300
           QFHV KAFEIAGEFI+ERL  +E       +++KEE +KDYLDVLLEFRGDGV EPS FS
Sbjct: 241 QFHVNKAFEIAGEFIKERLLLREKGNIIISDEKKEERKKDYLDVLLEFRGDGVVEPSQFS 300

Query: 301 SWIINVIVF 304
           SWIINVIVF
Sbjct: 301 SWIINVIVF 309

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BLAST of Lag0026972 vs. NCBI nr
Match: KAG7017413.1 (Cytochrome P450 76A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 486.1 bits (1250), Expect = 2.2e-133
Identity = 247/308 (80.19%), Postives = 264/308 (85.71%), Query Frame = 0


Query: 1   MEFEIAGFIFVSILWGFWVWRRRGSAAT-EQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIA  I VS LWG WVWRRRG+AA  EQLPPGP RWPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MEIEIAALILVSFLWGLWVWRRRGAAALGEQLPPGPTRWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFKNHDAPL+GRMIYEAMKG+HGTEGSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTIAREMFKNHDAPLSGRMIYEAMKGDHGTEGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGD---NGACGDAPPIELGRLF 180
           G HWRMLRRLAT+EFFVTRRLDAM+H+RRRCVDAMI++V D    GA   APP+++G+ F
Sbjct: 121 GSHWRMLRRLATSEFFVTRRLDAMQHVRRRCVDAMIQYVEDEIKGGAYWYAPPMDVGKFF 180

Query: 181 FIMSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           FIMSFNLIGNLIFSKDLV  NS RGK FYYH RRVME AG PNVADFL  FRWVDPQGIR
Sbjct: 181 FIMSFNLIGNLIFSKDLVGPNSKRGKEFYYHTRRVMETAGTPNVADFLRVFRWVDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFRGDGVEEPSCFSSW 300
           R TQFHVK+AFEIAGEFI+ER  EKE    +KEE +KDYLDVLLEFRGDGV EPS FSSW
Sbjct: 241 RTTQFHVKEAFEIAGEFIKERSMEKE----KKEERKKDYLDVLLEFRGDGVVEPSRFSSW 300

Query: 301 IINVIVFV 305
           IINVIV +
Sbjct: 301 IINVIVLL 304

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BLAST of Lag0026972 vs. NCBI nr
Match: XP_022982917.1 (cytochrome P450 76A2 [Cucurbita maxima])

HSP 1 Score: 484.6 bits (1246), Expect = 6.5e-133
Identity = 246/306 (80.39%), Postives = 263/306 (85.95%), Query Frame = 0


Query: 1   MEFEIAGFIFVSILWGFWVWRRRGSAAT-EQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIA  I VS LWG WVWRRRG+AA  EQLPPGPPRWPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MEIEIAALILVSFLWGLWVWRRRGAAALGEQLPPGPPRWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFKNHDA L+GRMIYEAMKG+HGTEGSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTIAREMFKNHDASLSGRMIYEAMKGDHGTEGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGD---NGACGDAPPIELGRLF 180
           G HWRMLRRLAT+EFFVTRRLDAM+H+RRRCVDAMI++V D     A   APP+++G+ F
Sbjct: 121 GSHWRMLRRLATSEFFVTRRLDAMQHVRRRCVDAMIQYVEDEIKGSAYWYAPPMDVGKFF 180

Query: 181 FIMSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           FIMSFNLIGNLIFSKDLV  NS RGK FYYH RRVME+AG PNVADFL  FRWVDPQGIR
Sbjct: 181 FIMSFNLIGNLIFSKDLVGPNSKRGKEFYYHTRRVMEIAGTPNVADFLRVFRWVDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFRGDGVEEPSCFSSW 300
           R TQFHVK+AFEIAGEFI+ER  EKE    +KEE +KDYLDVLLEFRGDGV EPS FSSW
Sbjct: 241 RTTQFHVKEAFEIAGEFIKERSTEKE----KKEERKKDYLDVLLEFRGDGVVEPSRFSSW 300

Query: 301 IINVIV 303
           IINVIV
Sbjct: 301 IINVIV 302

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BLAST of Lag0026972 vs. NCBI nr
Match: XP_004138005.1 (iridoid oxidase [Cucumis sativus] >KGN59047.1 hypothetical protein Csa_001475 [Cucumis sativus])

HSP 1 Score: 483.8 bits (1244), Expect = 1.1e-132
Identity = 237/312 (75.96%), Postives = 266/312 (85.26%), Query Frame = 0


Query: 1   MEFE-IAGFIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME E IAGFI +  LWG W+WR RG+ A  QLPPGPP WPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MELEIIAGFILLLFLWGLWLWRCRGAGADRQLPPGPPCWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFK HD PL+GRMIYEAMKGNHGT+GSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTVAREMFKTHDVPLSGRMIYEAMKGNHGTQGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDN-GACGDAPPIELGRLFFI 180
           GPHWRMLRRLAT+EFFV R+L++M+H+RR C+DAMIRHV +    CG+AP +++G+  F+
Sbjct: 121 GPHWRMLRRLATSEFFVARQLESMKHVRRHCIDAMIRHVEEGCDPCGNAPALDVGKFVFV 180

Query: 181 MSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRK 240
           M FNLIGNLIFSKDL+E NS RGK FYYH R+VMEMAGKPNVADFLPA RW+DPQGIRRK
Sbjct: 181 MGFNLIGNLIFSKDLLEANSKRGKEFYYHTRKVMEMAGKPNVADFLPALRWLDPQGIRRK 240

Query: 241 TQFHVKKAFEIAGEFIRERL--KEK------ESEEKRKEEGRKDYLDVLLEFRGDGVEEP 300
           TQFHV KAFEIAGEFI+ERL  +EK      + +EK++E G+KDYLDVLLEFRGDGVEEP
Sbjct: 241 TQFHVNKAFEIAGEFIKERLLIREKRDIINDDDDEKKEESGKKDYLDVLLEFRGDGVEEP 300

Query: 301 SCFSSWIINVIV 303
           S FSSWIINVIV
Sbjct: 301 SRFSSWIINVIV 312

[Close]

BLAST of Lag0026972 vs. NCBI nr
Match: XP_023528102.1 (cytochrome P450 76A2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 482.3 bits (1240), Expect = 3.2e-132
Identity = 244/306 (79.74%), Postives = 261/306 (85.29%), Query Frame = 0


Query: 1   MEFEIAGFIFVSILWGFWVWRRRGSAAT-EQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIA  I VS LWG WVWRRRG+AA  EQLPPGPPRWPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MEIEIAAVILVSFLWGLWVWRRRGAAALGEQLPPGPPRWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFKNHDAPL+GRMIYEAMKG+HGTEGSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTIAREMFKNHDAPLSGRMIYEAMKGDHGTEGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGD---NGACGDAPPIELGRLF 180
           G HWRMLRRLAT+EFFVTRRLDAM+H+RRRCVDAM+++V D     A   APP+++G+ F
Sbjct: 121 GSHWRMLRRLATSEFFVTRRLDAMQHVRRRCVDAMVQYVEDEIKGSAYWYAPPMDVGKFF 180

Query: 181 FIMSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           FIMSFNLIGNLIFSKDLV  NS RGK FYYH RRVME AG PNVADFL  FRW DPQGIR
Sbjct: 181 FIMSFNLIGNLIFSKDLVGPNSKRGKEFYYHTRRVMETAGTPNVADFLRVFRWFDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFRGDGVEEPSCFSSW 300
           R TQFHVK+AFEIAGEFI+ER  EKE    +KEE +KDYLDVLLEF GDGV EPS FSSW
Sbjct: 241 RTTQFHVKEAFEIAGEFIKERSMEKE----KKEERKKDYLDVLLEFHGDGVVEPSRFSSW 300

Query: 301 IINVIV 303
           IINVIV
Sbjct: 301 IINVIV 302

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BLAST of Lag0026972 vs. ExPASy Swiss-Prot
Match: W8JIS5 (Iridoid oxidase OS=Catharanthus roseus OX=4058 GN=IO PE=1 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.9e-59
Identity = 112/306 (36.60%), Postives = 193/306 (63.07%), Query Frame = 0


Query: 6   AGFIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPI 65
           AGF+ + I+   +V  R GS  +++ PPGPP WP+ GN+  L    H++  KL +KYGPI
Sbjct: 18  AGFLLLLII---FVKSRTGS--SKRKPPGPPGWPIFGNMFDLGDLPHQTLYKLKSKYGPI 77

Query: 66  MTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRM 125
           + L LGS++T+VV ++ +A E+FK HD P   R + + +   +  +GSL  + YG HWR+
Sbjct: 78  VWLQLGSINTMVVQNAVSAAELFKKHDVPFCDRKVPDTLTAFNFNQGSLGMNTYGGHWRV 137

Query: 126 LRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDNG----ACGDAPPIELGRLFFIMSF 185
           LRRL + EF V +R++    +RRR  D M+R + ++     A G    ++L R  F+M+F
Sbjct: 138 LRRLCSMEFLVNKRMNETTDLRRRIEDNMVRWIEEDSLASKAQGGTGAVQLSRFLFLMAF 197

Query: 186 NLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQF 245
           NL+GNL+ S+DL++     G+ F+     ++E+AG PN+ADFLP  + +DP G++++   
Sbjct: 198 NLVGNLMLSRDLMDNKDPEGREFFDCMNEILELAGTPNIADFLPLLKKLDPLGMKKRMVD 257

Query: 246 HVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFRGDGVEEPSCFSSWIINVI 305
           ++ +  +I+ +F++ERL   ++ +  K   +KD+LDV+LE++GDG + P  F+   +N++
Sbjct: 258 NMSRTMKISSKFVQERL---DNRKAGKINEKKDFLDVMLEYQGDGKDGPDKFTEQHVNIV 315

Query: 306 VFVSYY 308
           +   ++
Sbjct: 318 IMEMFF 315

[Close]

BLAST of Lag0026972 vs. ExPASy Swiss-Prot
Match: P37122 (Cytochrome P450 76A2 OS=Solanum melongena OX=4111 GN=CYP76A2 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 6.5e-51
Identity = 112/304 (36.84%), Postives = 172/304 (56.58%), Query Frame = 0


Query: 2   EFEIAGFIFVSILWGFWVW--RRRGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 61
           E+    F  + IL  F ++  ++  + ++ + PPGPP  P+ GN+ +L    ++    L 
Sbjct: 4   EWSYVFFSAIIILPAFILFFSQKNTTKSSYKFPPGPPGLPIFGNMFELGTEPYKKMAVLR 63

Query: 62  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 121
            KYGP++ L LGS  T+VV ++ A+ E+FKNHD   A R+I +  + +   +GSL  + Y
Sbjct: 64  QKYGPVLWLKLGSTYTMVVQTAQASEELFKNHDISFANRVIPDVNQAHSYYQGSLAIAPY 123

Query: 122 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHV-GDNGACGDAPPIELGRLFFI 181
           GP WR  RR+ T E FV +++     +RR+CVD M++ +  +  +      IE+ R  F+
Sbjct: 124 GPFWRFQRRICTIEMFVHKKISETEPVRRKCVDNMLKWIEKEANSAEKGSGIEVTRFVFL 183

Query: 182 MSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRK 241
            SFN++GNLI SKDL +  S     F+   +R+ E +G  NV+D  P  +  D Q +R+K
Sbjct: 184 ASFNMLGNLILSKDLADLESEEASEFFIAMKRINEWSGIANVSDIFPFLKKFDLQSLRKK 243

Query: 242 TQFHVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFRGDGVEEPSCFSSWII 301
               + KA EI   F    LKE+E E K+  E  KD+LDVLLEF+G G +EP+  S   I
Sbjct: 244 MARDMGKAVEIMSMF----LKEREEERKKGTEKGKDFLDVLLEFQGTGKDEPAKLSEHEI 303

Query: 302 NVIV 303
            + V
Sbjct: 304 KIFV 303

[Close]

BLAST of Lag0026972 vs. ExPASy Swiss-Prot
Match: Q8VWZ7 (Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.4e-44
Identity = 100/265 (37.74%), Postives = 147/265 (55.47%), Query Frame = 0


Query: 25  SAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPIMTLHLGSMSTVVVSSSTAA 84
           S  T+ LPPGP   P +G+L  L    H+S  KL+ K+GPIM+L LG ++T+V+SSST A
Sbjct: 24  SRRTKNLPPGPSPLPFIGSLHLLGDQPHKSLAKLSKKHGPIMSLKLGQITTIVISSSTMA 83

Query: 85  REMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRMLRRLATTEFFVTRRLDAMR 144
           +E+ +  D   + R +  A+  ++  + S++       WR LR++  +  F   RLDA +
Sbjct: 84  KEVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVASRWRSLRKVLNSNIFSGNRLDANQ 143

Query: 145 HIRRRCVDAMIRHVGDNGACGDAPPIELGRLFFIMSFNLIGNLIFSKDLVERNSTRGKGF 204
           H+R R V  +I +   N   G+A  +++GR  F  S NL+ NLIFSKDL +  S   K F
Sbjct: 144 HLRTRKVQELIAYCRKNSQSGEA--VDVGRAAFRTSLNLLSNLIFSKDLTDPYSDSAKEF 203

Query: 205 YYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFIRERLKEKESE 264
                 +M  AGKPN+ DF P    VDPQGIR +   H  +  ++ G  + ERL     E
Sbjct: 204 KDLVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEVLKLFGGLVNERL-----E 263

Query: 265 EKRKEEGRKDYLDVLLEFRGDGVEE 290
           ++R +  + D LDVLL    +  EE
Sbjct: 264 QRRSKGEKNDVLDVLLTTSQESPEE 281

[Close]

BLAST of Lag0026972 vs. ExPASy Swiss-Prot
Match: P37121 (Cytochrome P450 76A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP76A1 PE=2 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.5e-44
Identity = 102/266 (38.35%), Postives = 159/266 (59.77%), Query Frame = 0


Query: 42  GNLPQLSFSA-HESFTKLATKYGPIMTLHLG-SMSTVVVSSSTAAREMFKNHDAPLAGRM 101
           GN+  L+ SA ++    L  KYGPI+ L +G SM+T+V+ ++ +A E+F+NHD   + R 
Sbjct: 2   GNMFDLAGSAPYKKIACLKEKYGPILWLKIGSSMNTMVIQTANSASELFRNHDVSFSDRP 61

Query: 102 IYEAMKGNHGTEGSLITSQYGPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVG 161
           I +    ++  +GS+  + YG +WR  RR+ T E FV +R++   +IR+  VD M+R   
Sbjct: 62  IVDVNLAHNYYKGSMALAPYGNYWRFSRRICTVEMFVHKRINETTNIRQESVDKMLRLDE 121

Query: 162 DNGAC--GDAPPIELGRLFFIMSFNLIGNLIFSKDLV-ERNSTRGKGFYYHARRVMEMAG 221
           +  +   G    IE+ R  F+ SFN++GN+IFSKDLV +  S +G  F+     +ME AG
Sbjct: 122 EKASSSGGGGEGIEVTRYMFLASFNMVGNMIFSKDLVTDPESKQGSEFFNAMIGIMEWAG 181

Query: 222 KPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYL 281
            PN++D  P  +  D QG+R+K +  + K  EI  +FI ER++E++  EK +    KD L
Sbjct: 182 VPNISDIFPCLKMFDVQGLRKKMERDMGKGKEITKKFIEERIEERKKGEKNR--SIKDLL 241

Query: 282 DVLLEFRGDGVEEPSCFSSWIINVIV 303
           DVL++F G G +EP   S   I VI+
Sbjct: 242 DVLIDFEGSGKDEPDKLSEDEITVII 265

[Close]

BLAST of Lag0026972 vs. ExPASy Swiss-Prot
Match: D1MI46 (Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 1.2e-41
Identity = 108/283 (38.16%), Postives = 151/283 (53.36%), Query Frame = 0


Query: 7   GFIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPIM 66
           GF+F   L+    +  R S   + LPPGP   P++GNL  L    H+S  KLA K+GPIM
Sbjct: 11  GFLFTITLYQALNFFSRKS---KNLPPGPSPLPLIGNLHLLGDQPHKSLAKLAKKHGPIM 70

Query: 67  TLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRML 126
            L LG ++T+VV+SS  A+E+ +  D   + R I  A+  +   + S+I       WR L
Sbjct: 71  GLQLGQVTTIVVTSSGMAKEVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVASRWRGL 130

Query: 127 RRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDNGACGDAPPIELGRLFFIMSFNLIGN 186
           R+   +  F   RLDA +H+R R V  +I +   +   GDA  I++GR  F  S NL+ N
Sbjct: 131 RKALNSNMFSGNRLDANQHLRSRKVQELIAYCRKSSQTGDA--IDVGRAAFRTSLNLLSN 190

Query: 187 LIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKA 246
            +FSKDL +  S   K F      VM  AGKPN+ D+ P    VDPQGIR++   H  K 
Sbjct: 191 TMFSKDLTDPYSDSAKEFKDLVWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKI 250

Query: 247 FEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFRGDGVEE 290
            E+ G  I ERL++K+++         D LDVLL    +  EE
Sbjct: 251 LELFGGLIDERLQQKKAKGV-----NDDVLDVLLTTSEESPEE 283

[Close]

BLAST of Lag0026972 vs. ExPASy TrEMBL
Match: A0A6J1IXV7 (cytochrome P450 76A2 OS=Cucurbita maxima OX=3661 GN=LOC111481619 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 3.1e-133
Identity = 246/306 (80.39%), Postives = 263/306 (85.95%), Query Frame = 0


Query: 1   MEFEIAGFIFVSILWGFWVWRRRGSAAT-EQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIA  I VS LWG WVWRRRG+AA  EQLPPGPPRWPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MEIEIAALILVSFLWGLWVWRRRGAAALGEQLPPGPPRWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFKNHDA L+GRMIYEAMKG+HGTEGSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTIAREMFKNHDASLSGRMIYEAMKGDHGTEGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGD---NGACGDAPPIELGRLF 180
           G HWRMLRRLAT+EFFVTRRLDAM+H+RRRCVDAMI++V D     A   APP+++G+ F
Sbjct: 121 GSHWRMLRRLATSEFFVTRRLDAMQHVRRRCVDAMIQYVEDEIKGSAYWYAPPMDVGKFF 180

Query: 181 FIMSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           FIMSFNLIGNLIFSKDLV  NS RGK FYYH RRVME+AG PNVADFL  FRWVDPQGIR
Sbjct: 181 FIMSFNLIGNLIFSKDLVGPNSKRGKEFYYHTRRVMEIAGTPNVADFLRVFRWVDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFRGDGVEEPSCFSSW 300
           R TQFHVK+AFEIAGEFI+ER  EKE    +KEE +KDYLDVLLEFRGDGV EPS FSSW
Sbjct: 241 RTTQFHVKEAFEIAGEFIKERSTEKE----KKEERKKDYLDVLLEFRGDGVVEPSRFSSW 300

Query: 301 IINVIV 303
           IINVIV
Sbjct: 301 IINVIV 302

[Close]

BLAST of Lag0026972 vs. ExPASy TrEMBL
Match: A0A0A0LEE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G748270 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 5.4e-133
Identity = 237/312 (75.96%), Postives = 266/312 (85.26%), Query Frame = 0


Query: 1   MEFE-IAGFIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME E IAGFI +  LWG W+WR RG+ A  QLPPGPP WPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MELEIIAGFILLLFLWGLWLWRCRGAGADRQLPPGPPCWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFK HD PL+GRMIYEAMKGNHGT+GSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTVAREMFKTHDVPLSGRMIYEAMKGNHGTQGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDN-GACGDAPPIELGRLFFI 180
           GPHWRMLRRLAT+EFFV R+L++M+H+RR C+DAMIRHV +    CG+AP +++G+  F+
Sbjct: 121 GPHWRMLRRLATSEFFVARQLESMKHVRRHCIDAMIRHVEEGCDPCGNAPALDVGKFVFV 180

Query: 181 MSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRK 240
           M FNLIGNLIFSKDL+E NS RGK FYYH R+VMEMAGKPNVADFLPA RW+DPQGIRRK
Sbjct: 181 MGFNLIGNLIFSKDLLEANSKRGKEFYYHTRKVMEMAGKPNVADFLPALRWLDPQGIRRK 240

Query: 241 TQFHVKKAFEIAGEFIRERL--KEK------ESEEKRKEEGRKDYLDVLLEFRGDGVEEP 300
           TQFHV KAFEIAGEFI+ERL  +EK      + +EK++E G+KDYLDVLLEFRGDGVEEP
Sbjct: 241 TQFHVNKAFEIAGEFIKERLLIREKRDIINDDDDEKKEESGKKDYLDVLLEFRGDGVEEP 300

Query: 301 SCFSSWIINVIV 303
           S FSSWIINVIV
Sbjct: 301 SRFSSWIINVIV 312

[Close]

BLAST of Lag0026972 vs. ExPASy TrEMBL
Match: A0A6J1F4L5 (cytochrome P450 76A1 OS=Cucurbita moschata OX=3662 GN=LOC111442113 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 2.0e-132
Identity = 245/306 (80.07%), Postives = 261/306 (85.29%), Query Frame = 0


Query: 1   MEFEIAGFIFVSILWGFWVWRRRGSAAT-EQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIA  I VS LWG WVWRRRG+AA  EQLPPGP RWPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MEIEIAALILVSFLWGLWVWRRRGAAALGEQLPPGPTRWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFKNHDAPL+GRMIYEAMKG+HGTEGSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTIAREMFKNHDAPLSGRMIYEAMKGDHGTEGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGD---NGACGDAPPIELGRLF 180
           G HWRMLRRLAT+EFFVTRRLDAM+H+RRRCVDAMI++V D     A   APP+++G+ F
Sbjct: 121 GSHWRMLRRLATSEFFVTRRLDAMQHVRRRCVDAMIQYVEDEIKGSAYWYAPPMDVGKFF 180

Query: 181 FIMSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           FIMSFNLIGNLIFSKDLV  NS RGK FYYH RRVME AG PNVADFL  FRW DPQGIR
Sbjct: 181 FIMSFNLIGNLIFSKDLVGPNSKRGKEFYYHTRRVMETAGTPNVADFLRVFRWFDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFRGDGVEEPSCFSSW 300
           R TQFHVK+AFEIAGEFI+ER  EKE    +KEE +KDYLDVLLEFRGDGV EPS FSSW
Sbjct: 241 RTTQFHVKEAFEIAGEFIKERSMEKE----KKEERKKDYLDVLLEFRGDGVVEPSRFSSW 300

Query: 301 IINVIV 303
           IINVIV
Sbjct: 301 IINVIV 302

[Close]

BLAST of Lag0026972 vs. ExPASy TrEMBL
Match: A0A6J1CU75 (cytochrome P450 76A2 OS=Momordica charantia OX=3673 GN=LOC111014272 PE=3 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.6e-129
Identity = 237/311 (76.21%), Postives = 263/311 (84.57%), Query Frame = 0


Query: 1   MEFEIAGFIFVSILWGFWVWRRRGSA-ATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIAGFI VS LW  WVWRRRG+A  T QLPPGPPRWPVVGNLPQL +SAHESFTKLA
Sbjct: 1   MEIEIAGFILVSFLWALWVWRRRGAAEETGQLPPGPPRWPVVGNLPQLGWSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIM+LHLGSMSTVV+SSS AAREMFKNHDAPLAGR IYEAM+G+HGTEGS+I SQ+
Sbjct: 61  AKYGPIMSLHLGSMSTVVISSSEAAREMFKNHDAPLAGRKIYEAMRGDHGTEGSVIASQH 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDN---GACGDAPPIELGRLF 180
           GPHWRMLRRL TTEFFVTRRLDA +++RRRC+DAM+R + ++   GACG    I++G+L 
Sbjct: 121 GPHWRMLRRLLTTEFFVTRRLDATQNVRRRCIDAMLRFMEESSGGGACG----IDVGKLI 180

Query: 181 FIMSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           F+MSFNLIGNLIFS+DL+  NS RG  FYYHAR+VME AGKPNVADFLP FR VDPQGIR
Sbjct: 181 FVMSFNLIGNLIFSRDLLNPNSKRGNEFYYHARKVMETAGKPNVADFLPFFRRVDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFRGDGVEEPSCFSSW 300
           RKTQFHV KAFEIAGEFI ERL   E   +    G+KDYLDVLLEFRGDG E+PS FSSW
Sbjct: 241 RKTQFHVNKAFEIAGEFIGERLLAGEDGRR----GKKDYLDVLLEFRGDGEEQPSSFSSW 300

Query: 301 IINVIVFVSYY 308
           IINVIVFVS Y
Sbjct: 301 IINVIVFVSQY 303

[Close]

BLAST of Lag0026972 vs. ExPASy TrEMBL
Match: A0A5D3DNN5 (Cytochrome P450 76A2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003380 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.6e-127
Identity = 232/315 (73.65%), Postives = 264/315 (83.81%), Query Frame = 0


Query: 1   MEFEIAGFIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLAT 60
           ME EI    F+ +L+  W+WR R + A EQLPPGPP +PVVGNL QLSFSAHESFTKLA 
Sbjct: 1   MELEIIA-CFLLLLF-LWLWRCRRAGAREQLPPGPPCFPVVGNLLQLSFSAHESFTKLAA 60

Query: 61  KYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYG 120
           KYGPIMTL LGSM TVV+SSSTAAREMFK HDAPL+GRMIYEAMKG+HGT+GSLITSQYG
Sbjct: 61  KYGPIMTLQLGSMKTVVISSSTAAREMFKTHDAPLSGRMIYEAMKGDHGTQGSLITSQYG 120

Query: 121 PHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDN-GACGDAPPIELGRLFFIM 180
           PHWRMLRRLAT+EFFV R+L++M+H+RR C+DAMIRHV +    CG+A  +++G+  F+M
Sbjct: 121 PHWRMLRRLATSEFFVARQLESMKHVRRHCIDAMIRHVQEGCDPCGNAQALDVGKFMFVM 180

Query: 181 SFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKT 240
           SFNLIGNLIFSKDL+E NS RGK FYYH R+VMEMAGKPNVADFLPA RW+DPQGIRRKT
Sbjct: 181 SFNLIGNLIFSKDLLEANSKRGKEFYYHTRKVMEMAGKPNVADFLPALRWLDPQGIRRKT 240

Query: 241 QFHVKKAFEIAGEFIRERL---------KEKESEEKRKEEGRKDYLDVLLEFRGDGVEEP 300
           QFHV KAFEIAGEFI+ERL            + +EK +E  +KDYLDVLLEFRGDGVEEP
Sbjct: 241 QFHVNKAFEIAGEFIKERLLIRGKKDIINNDDDDEKIEEIRKKDYLDVLLEFRGDGVEEP 300

Query: 301 SCFSSWIINVIVFVS 306
           SCFSSWIINVIVFVS
Sbjct: 301 SCFSSWIINVIVFVS 313

[Close]

BLAST of Lag0026972 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 352.1 bits (902), Expect = 4.7e-97
Identity = 190/302 (62.91%), Postives = 230/302 (76.16%), Query Frame = 0


Query: 5   IAGFIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLS-FSAHESFTKLATKYG 64
           IA  I+V+ L  F+  R R      +LPPGP  WPV+GN+ QL+    H+S TKL+ ++G
Sbjct: 17  IAVLIYVTCL--FYTKRCR-----TRLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHG 76

Query: 65  PIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHW 124
           PIMTL +GSM TVV+SSS  ARE+FK HDA LAGR IYEAMKG   ++GSLIT+QYG +W
Sbjct: 77  PIMTLRIGSMLTVVISSSEVAREIFKKHDAALAGRKIYEAMKGGKSSDGSLITAQYGAYW 136

Query: 125 RMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDNGACGDAPPIELGRLFFIMSFNL 184
           RMLRRL TT+FFVTRRLDAM  +R RCVD M+R V + G  G    I++GR FF+M+FNL
Sbjct: 137 RMLRRLCTTQFFVTRRLDAMSDVRSRCVDQMLRFVEEGGQNG-TKTIDVGRYFFLMAFNL 196

Query: 185 IGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHV 244
           IGNL+FS+DL++ +S RG  F+YH  +VME AGKPNVADF P  R++DPQGIRRKTQFHV
Sbjct: 197 IGNLMFSRDLLDPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHV 256

Query: 245 KKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFR-GDGV-EEPSCFSSWIINVI 304
           +KAFEIAGEFIRER + +E E  + +E  KDYLDVLLEFR GDGV EEPS FSS  INVI
Sbjct: 257 EKAFEIAGEFIRERTEVRERE--KSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVI 308

[Close]

BLAST of Lag0026972 vs. TAIR 10
Match: AT3G52970.2 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 346.3 bits (887), Expect = 2.6e-95
Identity = 190/315 (60.32%), Postives = 230/315 (73.02%), Query Frame = 0


Query: 5   IAGFIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLS-FSAHESFTKLATKYG 64
           IA  I+V+ L  F+  R R      +LPPGP  WPV+GN+ QL+    H+S TKL+ ++G
Sbjct: 17  IAVLIYVTCL--FYTKRCR-----TRLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHG 76

Query: 65  PIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHW 124
           PIMTL +GSM TVV+SSS  ARE+FK HDA LAGR IYEAMKG   ++GSLIT+QYG +W
Sbjct: 77  PIMTLRIGSMLTVVISSSEVAREIFKKHDAALAGRKIYEAMKGGKSSDGSLITAQYGAYW 136

Query: 125 RMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDNGACG-------------DAPPI 184
           RMLRRL TT+FFVTRRLDAM  +R RCVD M+R V + G  G                 I
Sbjct: 137 RMLRRLCTTQFFVTRRLDAMSDVRSRCVDQMLRFVEEGGQNGMYYTINNIMTCIICTKTI 196

Query: 185 ELGRLFFIMSFNLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWV 244
           ++GR FF+M+FNLIGNL+FS+DL++ +S RG  F+YH  +VME AGKPNVADF P  R++
Sbjct: 197 DVGRYFFLMAFNLIGNLMFSRDLLDPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFL 256

Query: 245 DPQGIRRKTQFHVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEFR-GDGV-E 304
           DPQGIRRKTQFHV+KAFEIAGEFIRER + +E E  + +E  KDYLDVLLEFR GDGV E
Sbjct: 257 DPQGIRRKTQFHVEKAFEIAGEFIRERTEVRERE--KSDEKTKDYLDVLLEFRGGDGVDE 316

[Close]

BLAST of Lag0026972 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 162.2 bits (409), Expect = 6.9e-40
Identity = 94/288 (32.64%), Postives = 155/288 (53.82%), Query Frame = 0


Query: 3   FEIAGFIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKY 62
           F I  FI   +L+      RR      + PPGPPR P++GN+  +  + H SFT L+  Y
Sbjct: 10  FLIFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTY 69

Query: 63  GPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSL-ITSQYGP 122
           GP+M+L LG +++VV++S  A RE+ K HD  L+GR I EA K N+  E S+        
Sbjct: 70  GPVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNNHHEFSVGWIHPSSS 129

Query: 123 HWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDNGACGDAPPIELGRLFFIMSF 182
            +RMLR+L+ T+ F  + + A + +R + V  ++  + +  +C     +++  + F+ + 
Sbjct: 130 RFRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSE--SCEREEAVDISHVSFVTAL 189

Query: 183 NLIGNLIFSKDLVERNSTRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQF 242
           N+I N++FS +L   +S     F        E  G P++A+F P  R++D QG  +K + 
Sbjct: 190 NIISNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKKMRE 249

Query: 243 HVKKAFEIAGEFIRERLKEKESEEKRKEEGRKDYLDVLLEF-RGDGVE 289
              +  ++  EF   R+ EK S    K+   KD+LDVL++  +GD  E
Sbjct: 250 SSGRLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETE 295

[Close]

BLAST of Lag0026972 vs. TAIR 10
Match: AT2G45580.1 (cytochrome P450, family 76, subfamily C, polypeptide 3 )

HSP 1 Score: 160.2 bits (404), Expect = 2.6e-39
Identity = 95/277 (34.30%), Postives = 151/277 (54.51%), Query Frame = 0


Query: 8   FIFVSILWGFWVWRRRGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPIMT 67
           F+     + F   + R S++T  LPPGPP  P+VGN+ QL F+ H S    +  YGPIM+
Sbjct: 15  FLLTLFFFFFATAKTRRSSSTGTLPPGPPILPLVGNIFQLGFNPHRSLAAFSKTYGPIMS 74

Query: 68  LHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRMLR 127
           L LG ++ VV+SS  AA+E  + HD  ++ R   +A++     + S++       WR L+
Sbjct: 75  LKLGRLTAVVISSPEAAKEALRTHDHVMSARTFNDALRAFDHHKHSIVWIPPSARWRFLK 134

Query: 128 RLATTEFFVTRRLDAMRHIRRRCVDAMIRHVGDNGACGDAPPIELGRLFFIMSFNLIGNL 187
           +  T      + LDA++ +R R V+ ++  V +    G+A  I+L R  F+ SFN+I N 
Sbjct: 135 KTITKYLLSPQNLDAIQSLRMRKVEELVSLVNEFRERGEA--IDLARASFVTSFNIISNA 194

Query: 188 IFSKDLVERNSTRGK-GFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKA 247
           +FS DL   +S      F+     + ++AG PNV D+    R++D QG R+K    ++K 
Sbjct: 195 LFSVDLATYDSNSSSYEFHNTVVHLTDIAGIPNVGDYFQYMRFLDLQGTRKKAVLCIEKL 254

Query: 248 FEIAGEFIRERLKEK--ESEEKRKEEGRKDYLDVLLE 282
           F +  EFI  RL ++   +E++ KE    D LD LL+
Sbjct: 255 FRVFQEFIDARLAKRFSRTEKEPKEASSIDMLDSLLD 289

[Close]

BLAST of Lag0026972 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 159.1 bits (401), Expect = 5.8e-39
Identity = 86/268 (32.09%), Postives = 151/268 (56.34%), Query Frame = 0


Query: 23  RGSAATEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPIMTLHLGSMSTVVVSSST 82
           R S     LPPGPPR P++GN+  +    H SF +L+  YGP+M+L LGS++TVV++S  
Sbjct: 30  RISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPVMSLKLGSLNTVVIASPE 89

Query: 83  AAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLI-TSQYGPHWRMLRRLATTEFFVTRRLD 142
           AARE+ + HD  L+ R    A++  +  + SL+        WR+LRRL+ T+    +R++
Sbjct: 90  AAREVLRTHDQILSARSPTNAVRSINHQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIE 149

Query: 143 AMRHIRRRCVDAMIRHVGDNGACGDAPPIELGRLFFIMSFNLIGNLIFSKDLVERNSTRG 202
           A + +R   V  ++  + ++    ++  +++ R+ FI + N+I N++FS DL   N+   
Sbjct: 150 ATKALRMNKVKELVSFISESSDREES--VDISRVAFITTLNIISNILFSVDLGSYNAKAS 209

Query: 203 -KGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFIRERLKE 262
             G       VM+ AG P+ A++ P  R++D QG  +  +   ++   +   FI  ++ E
Sbjct: 210 INGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCTERLVRVFRGFIDAKIAE 269

Query: 263 KESEEKRKEEGRKDYLDVLLEFRGDGVE 289
           K S+   K+  + D++D LL+++GD  E
Sbjct: 270 KSSQNNPKDVSKNDFVDNLLDYKGDESE 295

The following BLAST results are available for this feature:

BLAST of Lag0026972 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905939.1	1.5e-134	77.35	iridoid oxidase [Benincasa hispida]	[more]
KAG7017413.1	2.2e-133	80.19	Cytochrome P450 76A2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022982917.1	6.5e-133	80.39	cytochrome P450 76A2 [Cucurbita maxima]	[more]
XP_004138005.1	1.1e-132	75.96	iridoid oxidase [Cucumis sativus] >KGN59047.1 hypothetical protein Csa_001475 [C...	[more]
XP_023528102.1	3.2e-132	79.74	cytochrome P450 76A2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0026972 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JIS5	2.9e-59	36.60	Iridoid oxidase OS=Catharanthus roseus OX=4058 GN=IO PE=1 SV=1	[more]
P37122	6.5e-51	36.84	Cytochrome P450 76A2 OS=Solanum melongena OX=4111 GN=CYP76A2 PE=2 SV=1	[more]
Q8VWZ7	3.4e-44	37.74	Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1	[more]
P37121	4.5e-44	38.35	Cytochrome P450 76A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP76A1 PE=2 SV=...	[more]
D1MI46	1.2e-41	38.16	Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1	[more]

BLAST of Lag0026972 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IXV7	3.1e-133	80.39	cytochrome P450 76A2 OS=Cucurbita maxima OX=3661 GN=LOC111481619 PE=3 SV=1	[more]
A0A0A0LEE2	5.4e-133	75.96	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G748270 PE=3 SV=1	[more]
A0A6J1F4L5	2.0e-132	80.07	cytochrome P450 76A1 OS=Cucurbita moschata OX=3662 GN=LOC111442113 PE=3 SV=1	[more]
A0A6J1CU75	1.6e-129	76.21	cytochrome P450 76A2 OS=Momordica charantia OX=3673 GN=LOC111014272 PE=3 SV=1	[more]
A0A5D3DNN5	2.6e-127	73.65	Cytochrome P450 76A2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352...	[more]

BLAST of Lag0026972 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52970.1	4.7e-97	62.91	cytochrome P450, family 76, subfamily G, polypeptide 1	[more]
AT3G52970.2	2.6e-95	60.32	cytochrome P450, family 76, subfamily G, polypeptide 1	[more]
AT1G33720.1	6.9e-40	32.64	cytochrome P450, family 76, subfamily C, polypeptide 6	[more]
AT2G45580.1	2.6e-39	34.30	cytochrome P450, family 76, subfamily C, polypeptide 3	[more]
AT2G45560.1	5.8e-39	32.09	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 59..78 score: 39.26 coord: 83..104 score: 20.17 coord: 179..197 score: 18.7
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 32..283 e-value: 9.1E-31 score: 107.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 25..295 e-value: 3.6E-47 score: 163.0
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 18..285
None	No IPR available	PANTHER	PTHR24299:SF22	CYTOCHROME P450 76A1-LIKE	coord: 1..304
None	No IPR available	PANTHER	PTHR24299	CYTOCHROME P450 FAMILY 1	coord: 1..304

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0026972.1	Lag0026972.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen