Lag0026185 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0026185
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	cysteine proteinase inhibitor 5-like
Location	chr10: 31769039 .. 31769395 (-)
RNA-Seq Expression	Lag0026185
Synteny	Lag0026185

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATCCCGCTTCGGTTCTCTGTTCCTCATCCTTGTCCTCCTCTCGATGGTGGTGGCCGGCATGGGCGTTTATCGAAAGGGGATTCCCGGCGGGTGGGGTCCGATTGAGGATGTGAAGGATCCGTACCTTCAAGACATCGCAAAGTTCGCGGTCACTGAGTACAACAAAATTTCCAATGGCGGACTCGTGTTCGAATCCATCCTCAGCGGCGAATCGCAGGCCGTCGATGGAGTCAACTACCGCTTCGTTCTCGACGTCAAGAATGGCGATTCGGATGCCAAATATGAGGCCGTCGTCTATGTCAGGGCATGGGAGAATTACAGGGAACTCACCTCCTTCAACCCCCTCGCCTGA

mRNA sequence

ATGAAATCCCGCTTCGGTTCTCTGTTCCTCATCCTTGTCCTCCTCTCGATGGTGGTGGCCGGCATGGGCGTTTATCGAAAGGGGATTCCCGGCGGGTGGGGTCCGATTGAGGATGTGAAGGATCCGTACCTTCAAGACATCGCAAAGTTCGCGGTCACTGAGTACAACAAAATTTCCAATGGCGGACTCGTGTTCGAATCCATCCTCAGCGGCGAATCGCAGGCCGTCGATGGAGTCAACTACCGCTTCGTTCTCGACGTCAAGAATGGCGATTCGGATGCCAAATATGAGGCCGTCGTCTATGTCAGGGCATGGGAGAATTACAGGGAACTCACCTCCTTCAACCCCCTCGCCTGA

Coding sequence (CDS)

ATGAAATCCCGCTTCGGTTCTCTGTTCCTCATCCTTGTCCTCCTCTCGATGGTGGTGGCCGGCATGGGCGTTTATCGAAAGGGGATTCCCGGCGGGTGGGGTCCGATTGAGGATGTGAAGGATCCGTACCTTCAAGACATCGCAAAGTTCGCGGTCACTGAGTACAACAAAATTTCCAATGGCGGACTCGTGTTCGAATCCATCCTCAGCGGCGAATCGCAGGCCGTCGATGGAGTCAACTACCGCTTCGTTCTCGACGTCAAGAATGGCGATTCGGATGCCAAATATGAGGCCGTCGTCTATGTCAGGGCATGGGAGAATTACAGGGAACTCACCTCCTTCAACCCCCTCGCCTGA

Protein sequence

MKSRFGSLFLILVLLSMVVAGMGVYRKGIPGGWGPIEDVKDPYLQDIAKFAVTEYNKISNGGLVFESILSGESQAVDGVNYRFVLDVKNGDSDAKYEAVVYVRAWENYRELTSFNPLA

Homology

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BLAST of Lag0026185 vs. NCBI nr
Match: XP_022973098.1 (cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine proteinase inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 130.2 bits (326), Expect = 1.2e-26
Identity = 64/118 (54.24%), Postives = 88/118 (74.58%), Query Frame = 0


Query: 1   MKSRFGSLFLILVLLSMVVAGMGVYRKGIPGGWGPIEDVKDPYLQDIAKFAVTEYNKISN 60
           MKSR  SLFLIL+L  +VVA   + R  + GGW  I+DVKDP++Q+I KFAV+EYNK S 
Sbjct: 1   MKSRSASLFLILLLPLLVVATARMGR--LVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSK 60

Query: 61  GGLVFESILSGESQAVDGVNYRFVLDVKNGDSDAKYEAVVYVRAWENYRELTSFNPLA 119
           G L F+ ++ GESQ V G+NYR V++ K G+S  KY+A+V+ +AW+++ ELTSF P+A
Sbjct: 61  GALEFKDVVKGESQVVSGMNYRLVIEAKKGESIGKYQALVWEKAWQHFMELTSFKPVA 116

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BLAST of Lag0026185 vs. NCBI nr
Match: XP_023520814.1 (cysteine proteinase inhibitor 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 129.8 bits (325), Expect = 1.6e-26
Identity = 64/118 (54.24%), Postives = 87/118 (73.73%), Query Frame = 0


Query: 1   MKSRFGSLFLILVLLSMVVAGMGVYRKGIPGGWGPIEDVKDPYLQDIAKFAVTEYNKISN 60
           MKSR  SLFLIL+L  +VVA   + R  + GGW  I+DVKDP++Q+I KFAV+EYNK S 
Sbjct: 1   MKSRSASLFLILLLPLLVVATARMGR--LVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSK 60

Query: 61  GGLVFESILSGESQAVDGVNYRFVLDVKNGDSDAKYEAVVYVRAWENYRELTSFNPLA 119
           G L F+ ++ GESQ V G NYR V++ K G+S  KY+A+V+ +AW+++ ELTSF P+A
Sbjct: 61  GALEFKDVVKGESQVVSGTNYRLVIEAKKGESIGKYQALVWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Lag0026185 vs. NCBI nr
Match: XP_022927193.1 (cysteine proteinase inhibitor 5-like [Cucurbita moschata])

HSP 1 Score: 129.8 bits (325), Expect = 1.6e-26
Identity = 63/118 (53.39%), Postives = 88/118 (74.58%), Query Frame = 0


Query: 1   MKSRFGSLFLILVLLSMVVAGMGVYRKGIPGGWGPIEDVKDPYLQDIAKFAVTEYNKISN 60
           MKSR  SLFLIL+L  +VVA   + R  + GGW  I+DVKDP++Q+I KFAV+EYNK S 
Sbjct: 1   MKSRSASLFLILLLPLLVVATARMGR--LVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSK 60

Query: 61  GGLVFESILSGESQAVDGVNYRFVLDVKNGDSDAKYEAVVYVRAWENYRELTSFNPLA 119
           G L F+ ++ GESQ V G+NYR V++ K G+S  KY+A+++ +AW+++ ELTSF P+A
Sbjct: 61  GALEFKDVVKGESQVVSGMNYRLVIEAKKGESIGKYQALIWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Lag0026185 vs. NCBI nr
Match: XP_038894857.1 (cysteine proteinase inhibitor 1 [Benincasa hispida])

HSP 1 Score: 122.9 bits (307), Expect = 1.9e-24
Identity = 66/121 (54.55%), Postives = 85/121 (70.25%), Query Frame = 0


Query: 1   MKSRFGSLFLILV--LLSMVVAGMGVYRKGIPGGWGPIEDVKDPYLQDIAKFAVTEYNKI 60
           M SR  SLFLIL+  LLS VVA  G     + GGW  I+DV DP++Q+IA FAVTEYNK 
Sbjct: 1   MNSRSASLFLILLLPLLSAVVARKG----ALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQ 60

Query: 61  SNGGLV-FESILSGESQAVDGVNYRFVLDVKNGDSDAKYEAVVYVRAWENYRELTSFNPL 119
           S G  + F+S++SGE+Q V G+NYR V+D K G+S  KY A+V+ + WEN ++LTSF P 
Sbjct: 61  SKGVTIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKPF 117

[Close]

BLAST of Lag0026185 vs. NCBI nr
Match: XP_022142402.1 (cysteine proteinase inhibitor 5-like [Momordica charantia])

HSP 1 Score: 120.9 bits (302), Expect = 7.2e-24
Identity = 62/120 (51.67%), Postives = 83/120 (69.17%), Query Frame = 0


Query: 1   MKSRFGSLFLILVLLSMVVAGMGVYRKG-IPGGWGPIEDVKDPYLQDIAKFAVTEYNKIS 60
           MKSR   L L+L+L +MV A     RKG + GGW PI DVKDP++Q+I KFAV  YN  S
Sbjct: 7   MKSRSAPLLLLLLLPAMVAAVAVAARKGPLVGGWTPITDVKDPHVQEIGKFAVAAYNTQS 66

Query: 61  NGGLVFESILSGESQAVDGVNYRFVLDVKN-GDSDAKYEAVVYVRAWENYRELTSFNPLA 119
            G + F+ + SGE+Q V G NYR V+  K+ G S AKY+A+V+ + WEN+++LTSF P+A
Sbjct: 67  KGAIKFDRVESGETQVVSGTNYRLVVVAKDGGGSTAKYQAIVWEKPWENFKKLTSFKPVA 126

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BLAST of Lag0026185 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 99.0 bits (245), Expect = 3.8e-20
Identity = 51/113 (45.13%), Postives = 72/113 (63.72%), Query Frame = 0


Query: 7   SLFLILVLLSMVVAGMGVYRKGIP--GGWGPIEDVKDPYLQDIAKFAVTEYNKISNGGLV 66
           S  + L+LLS+VV  + +Y       GGW PI +V DP + +I +FAV+EYNK S  GL 
Sbjct: 3   SKVVFLLLLSLVVVLLPLYASAAARVGGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLK 62

Query: 67  FESILSGESQAVDGVNYRFVLDVKNGDSDAK-YEAVVYVRAWENYRELTSFNP 117
           FE+++SGE+Q V G NYR  +   +GD  +K Y A+V+ + W  +R LTSF P
Sbjct: 63  FETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]

BLAST of Lag0026185 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 9.5e-19
Identity = 45/105 (42.86%), Postives = 71/105 (67.62%), Query Frame = 0


Query: 10  LILVLLSMVVAGMGVYRKGIPGGWGPIEDVKDPYLQDIAKFAVTEYNKISNGGLVFESIL 69
           L+L+LL++  A +G  ++   GGW PIE++    +QD+A+FAV+E+NK +N  L ++S++
Sbjct: 9   LLLLLLALSAAVVGGRKRVAAGGWRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVV 68

Query: 70  SGESQAVDGVNYRFVLDVKNGDSDAKYEAVVYVRAWENYRELTSF 115
            G +Q V G NYR V+  K+G     YEAVV+ + W ++R LTSF
Sbjct: 69  RGYTQVVAGTNYRLVIAAKDGAVVGNYEAVVWDKPWMHFRNLTSF 113

[Close]

BLAST of Lag0026185 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 8.0e-18
Identity = 47/107 (43.93%), Postives = 68/107 (63.55%), Query Frame = 0


Query: 8   LFLILVLLSMVVAGMGVYRKGIPGGWGPIEDVKDPYLQDIAKFAVTEYNKISNGGLVFES 67
           LFL+L L + VV G  +      GGW PIE +    +QD+A+FAV+E+NK +N  L ++S
Sbjct: 10  LFLLLALSAAVVGGRKLV---AAGGWRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQS 69

Query: 68  ILSGESQAVDGVNYRFVLDVKNGDSDAKYEAVVYVRAWENYRELTSF 115
           ++ G +Q V G NYR V+  K+G     YEAVV+ + W ++R LTSF
Sbjct: 70  VVRGYTQVVAGTNYRLVIAAKDGAVVGNYEAVVWDKPWMHFRNLTSF 113

[Close]

BLAST of Lag0026185 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 3.0e-17
Identity = 48/114 (42.11%), Postives = 68/114 (59.65%), Query Frame = 0


Query: 8   LFLILVLLSMVVAGMGVYRKGIP--GGWGPIEDVKDPYLQDIAKFAVTEYNKISNGGLVF 67
           L L+L + +   A +G  R   P  GGW PI DV DP++Q++  +AV  +  +S+ GL F
Sbjct: 8   LALLLAVSAAAAAQVGGNRGHGPLVGGWSPITDVGDPHIQELGGWAVERHASLSSDGLRF 67

Query: 68  ESILSGESQAVDGVNYRFVLDVKN-GDSDAKYEAVVYVRAWENYRELTSFNPLA 119
             + SGE Q V G+NYR V+   +   + A Y AVVY ++W N R+LTSF P A
Sbjct: 68  RRVTSGEQQVVSGMNYRLVVSASDPAGATASYVAVVYEQSWTNTRQLTSFKPAA 121

[Close]

BLAST of Lag0026185 vs. ExPASy Swiss-Prot
Match: Q10Q46 (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.1e-14
Identity = 35/90 (38.89%), Postives = 58/90 (64.44%), Query Frame = 0


Query: 29  IPGGWGPIEDVKDPYLQDIAKFAVTEYNKIS-NGGLVFESILSGESQAVDGVNYRFVLDV 88
           +P GW PI+++ DP++Q++ ++A+TE N++S +  L F  +  GE Q V G+NYR  ++ 
Sbjct: 19  LPRGWSPIKNIDDPHIQELGRWAITENNRVSPSDELTFHRVTGGEQQVVSGMNYRLEIEA 78

Query: 89  KNGDSD--AKYEAVVYVRAWENYRELTSFN 116
            +G  D    Y AVV+ + W N R+L SF+
Sbjct: 79  ASGGGDVTGSYGAVVFEQEWSNTRKLISFD 108

The following BLAST results are available for this feature:

BLAST of Lag0026185 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022973098.1	1.2e-26	54.24	cysteine proteinase inhibitor 5-like [Cucurbita maxima] >KAG6583727.1 Cysteine p...	[more]
XP_023520814.1	1.6e-26	54.24	cysteine proteinase inhibitor 5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022927193.1	1.6e-26	53.39	cysteine proteinase inhibitor 5-like [Cucurbita moschata]	[more]
XP_038894857.1	1.9e-24	54.55	cysteine proteinase inhibitor 1 [Benincasa hispida]	[more]
XP_022142402.1	7.2e-24	51.67	cysteine proteinase inhibitor 5-like [Momordica charantia]	[more]

BLAST of Lag0026185 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41916	3.8e-20	45.13	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
P86472	9.5e-19	42.86	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]
Q6TPK4	8.0e-18	43.93	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
Q10J94	3.0e-17	42.11	Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]
Q10Q46	1.1e-14	38.89	Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 28..117 e-value: 6.6E-15 score: 65.5
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 36..116 e-value: 1.6E-27 score: 95.7
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 31..116 e-value: 8.7851E-16 score: 65.5732
None	No IPR available	GENE3D	3.10.450.10		coord: 17..118 e-value: 2.1E-30 score: 106.8
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 1..116
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 26..116

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0026185.1	Lag0026185.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity