Lag0022623 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0022623 vs. NCBI nr
Match: XP_022145171.1 (putative disease resistance protein RGA3 [Momordica charantia] >XP_022145172.1 putative disease resistance protein RGA3 [Momordica charantia] >XP_022145173.1 putative disease resistance protein RGA3 [Momordica charantia] >XP_022145174.1 putative disease resistance protein RGA3 [Momordica charantia] >XP_022145175.1 putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 412.5 bits (1059), Expect = 3.3e-111
Identity = 225/315 (71.43%), Postives = 247/315 (78.41%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGL+KELSKLR+ LL AE IL DINRK+L HE
Sbjct: 1   MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLDKELSKLRKRLLKAETILGDINRKKLRHE 60

Query: 87  SVSMWVDNLQDIVYEADDLLDEL---------------------------------MAKQ 146
           SV +WV++LQ +VY+ADDLLDEL                                 MA  
Sbjct: 61  SVRLWVEDLQHLVYQADDLLDELVYEDLRRKVEIEKINKVRNFFSLTNNPFLFRSKMANS 120

Query: 147 MKDITETLDRHYLKASSLGVVGLVGEESTET-NKNIDLMQFRETTSILNFEVIGREVEAS 206
           MK I+ETL +HY +AS+L   GLVGEE+TET NKNI L Q RETTSILNFEVIGRE+E  
Sbjct: 121 MKAISETLYQHYYEASAL---GLVGEETTETDNKNI-LKQIRETTSILNFEVIGREIEVL 180

Query: 207 NIVKLVIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIIT 266
           NIVKLVIDSS +H MSIIPIVGMGG+GKTTLAK++FNHEL+K HFDK+IWVCVSE FII 
Sbjct: 181 NIVKLVIDSSYEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKSIWVCVSESFIII 240

Query: 267 NILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKE 308
           NILEGIFQSLTNTS GL  KE L++ LQKEMQGK YFLVLDDVWNEE SLWGELRDCL E
Sbjct: 241 NILEGIFQSLTNTSSGLNYKETLLQRLQKEMQGKKYFLVLDDVWNEEVSLWGELRDCLNE 300

BLAST of Lag0022623 vs. NCBI nr
Match: XP_038877313.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 409.1 bits (1050), Expect = 3.6e-110
Identity = 217/326 (66.56%), Postives = 254/326 (77.91%), Query Frame = 0

Query: 21  ESKPREMAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINR 80
           + K + MAEFLW FAVQEVLKK +K+AAE+I +AWG +KELSKLR+ LL +EAILRDIN+
Sbjct: 118 KQKAKRMAEFLWIFAVQEVLKKTVKVAAEEISMAWGFKKELSKLRKCLLKSEAILRDINK 177

Query: 81  KRLHHESVSMWVDNLQDIVYEADDLLDEL------------------------------- 140
           K+LHHESV MWV++LQ  VYE DDLLDEL                               
Sbjct: 178 KKLHHESVRMWVEDLQHFVYEVDDLLDELVYEHLRQKVDTEKRNKKVRNFFSSSNNPFPF 237

Query: 141 ---MAKQMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLMQFRETTSILNFE--V 200
              MAK+MKDITETL +HY +AS LG+V L+GEES ET+KNI+L Q RETTS LNFE  +
Sbjct: 238 RRKMAKRMKDITETLYKHYCEASPLGLVALLGEESIETHKNINLKQIRETTSNLNFEEVI 297

Query: 201 IGREVEASNIVKLVIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVC 260
           IGREVE SNIVK+VI+SSN+H MSIIPIVGMGG+G+TTLAK++FNHELVK HFD+TIWVC
Sbjct: 298 IGREVEVSNIVKMVIESSNEHPMSIIPIVGMGGLGETTLAKMVFNHELVKDHFDETIWVC 357

Query: 261 VSEPFIITNILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWG 311
           VSEPFII NILEG+FQSLT TS GL +KENL+R LQ+EMQGK+Y LVLD VWNEEA LWG
Sbjct: 358 VSEPFIILNILEGMFQSLTKTSSGL-NKENLLRKLQEEMQGKSYLLVLDYVWNEEARLWG 417

BLAST of Lag0022623 vs. NCBI nr
Match: XP_038896003.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 399.4 bits (1025), Expect = 2.9e-107
Identity = 213/316 (67.41%), Postives = 240/316 (75.95%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAEFLWTFAVQEVLKKVLK+AAE+IGVA G  KELS+LRE L +AEAILR IN K+ HHE
Sbjct: 1   MAEFLWTFAVQEVLKKVLKVAAEEIGVALGFHKELSELREWLCNAEAILRGINTKKPHHE 60

Query: 87  SVSMWVDNLQDIVYEADDLLDEL---------------------------------MAKQ 146
           SV MWV +LQ IVYEADDLLDEL                                 MAK+
Sbjct: 61  SVGMWVGDLQRIVYEADDLLDELVYEDLRRKVETEKRKKVRNFFSSSNNPFLFRLKMAKR 120

Query: 147 MKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLMQFRETTSILNFE--VIGREVEA 206
           MK I E L +HY KA  LG++   GEESTET+KNIDL Q RETTS       +IGREVE 
Sbjct: 121 MKYIIEALHKHYTKAGPLGLIAQAGEESTETHKNIDLKQIRETTSKFKSREVIIGREVEV 180

Query: 207 SNIVKLVIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFII 266
           SNIVKLV+DSSN+H MSIIPIVGMGG+GKTTLAK++FNHELV+GHFDKTIW+CVSEPFI+
Sbjct: 181 SNIVKLVVDSSNEHPMSIIPIVGMGGLGKTTLAKMVFNHELVQGHFDKTIWICVSEPFIV 240

Query: 267 TNILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLK 308
            NILEGIFQSLT T     +KENL++ LQ+EMQGKNYFLVLDDVWNEEA+LW +L+DCL 
Sbjct: 241 INILEGIFQSLTKTWSTGLNKENLLQRLQEEMQGKNYFLVLDDVWNEEATLWDDLKDCLN 300

BLAST of Lag0022623 vs. NCBI nr
Match: XP_022145177.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 397.9 bits (1021), Expect = 8.3e-107
Identity = 208/314 (66.24%), Postives = 244/314 (77.71%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAEFLWTFAVQEVL+K++K+AA+QIG+AWGLEKELSKL + LL AE IL DINR++LHH+
Sbjct: 1   MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHHD 60

Query: 87  SVSMWVDNLQDIVYEADDLLDEL---------------------------------MAKQ 146
           SV +W+++LQD+V+EA DLLDEL                                 MAK+
Sbjct: 61  SVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVEIGKMNKVRGSISRSYNLFSFRRKMAKK 120

Query: 147 MKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLMQFRETTSILNFEVIGREVEASN 206
           MKD+TETL +HY +AS L   GLVG+ES ETN+ I L Q RETTS LNFEV+GRE+E SN
Sbjct: 121 MKDVTETLYKHYCEASPL---GLVGDESIETNQ-IVLKQIRETTSNLNFEVVGREIEVSN 180

Query: 207 IVKLVIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITN 266
           IVKLVIDSSN+H  SIIPIVGMGG+GKTTLA++IFNHE++KGHFDKTIW+CVSEPFII N
Sbjct: 181 IVKLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIIIN 240

Query: 267 ILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEI 308
           ILEGIFQ LT TS GL SKE LV+ LQ+EM GK YFLVLDDVWNEE+ LW +L DCLK+I
Sbjct: 241 ILEGIFQGLTKTSSGLNSKETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQI 300

BLAST of Lag0022623 vs. NCBI nr
Match: XP_022145229.1 (putative disease resistance protein RGA4 [Momordica charantia])

HSP 1 Score: 397.9 bits (1021), Expect = 8.3e-107
Identity = 214/314 (68.15%), Postives = 239/314 (76.11%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAEFLWTFAVQEVLKKVLKLAAEQIGVAW L+KELSKLR+ LL AE IL DINR++L HE
Sbjct: 1   MAEFLWTFAVQEVLKKVLKLAAEQIGVAWALDKELSKLRKRLLKAETILGDINRRKLCHE 60

Query: 87  SVSMWVDNLQDIVYEADDLLDEL---------------------------------MAKQ 146
           SV +WV++LQ +VY+ADDLLD+L                                 MAK+
Sbjct: 61  SVRLWVEDLQHLVYQADDLLDDLVYEDLRQKVEMEKINKVRNFLSPSNNPFLFRFKMAKR 120

Query: 147 MKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLMQFRETTSILNFEVIGREVEASN 206
           MK I+ETL  HY +AS   V+G VGEESTET     L Q RETTSILNF+VIGRE+E   
Sbjct: 121 MKAISETLYEHYCEAS---VLGFVGEESTETTYKNILRQIRETTSILNFKVIGREIEVLK 180

Query: 207 IVKLVIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITN 266
           I+KLVIDSS++H MSIIPIVGMGG+GKTTLAK++FNHEL+K HFDKTIWVCVS+PFII N
Sbjct: 181 IMKLVIDSSHEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKTIWVCVSKPFIIVN 240

Query: 267 ILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEI 308
           ILEGIFQSLTNTS GL  KE L   LQKEMQGK YFLVLDDVWNEE +LW ELRDCL EI
Sbjct: 241 ILEGIFQSLTNTSSGLNYKETLFHRLQKEMQGKKYFLVLDDVWNEEVALWDELRDCLNEI 300

BLAST of Lag0022623 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 157.9 bits (398), Expect = 1.9e-37
Identity = 98/295 (33.22%), Postives = 157/295 (53.22%), Query Frame = 0

Query: 36  VQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHESVSMWVDNL 95
           +Q +L  +      ++G+ +G EKE  KL       +A+L D   K+L ++++  W+  L
Sbjct: 6   LQVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKL 65

Query: 96  QDIVYEADDLLDELMAKQMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLM---- 155
               YE DD+LD+   +  +     L R++ +  ++     VG+   E  + +D +    
Sbjct: 66  NVAAYEVDDILDDCKTEAARFKQAVLGRYHPR--TITFCYKVGKRMKEMMEKLDAIAEER 125

Query: 156 --------------QFRETTSILNF-EVIGREVEASNIVKLVIDS-SNQHLMSIIPIVGM 215
                           R+T  +L   +V GRE E   IVK++I++ S    + ++PI+GM
Sbjct: 126 RNFHLDERIIERQAARRQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPILGM 185

Query: 216 GGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITNILEGIFQSLTNTSGGLKSKENL 275
           GG+GKTTLA+++FN + +  HF+  IWVCVS+ F    +++ I +S+   S G      L
Sbjct: 186 GGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLAPL 245

Query: 276 VRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEIAGKSGNVIIVTTRSEKL 311
            + LQ+ + GK YFLVLDDVWNE+   W  LR  LK   G SG  I++TTR EK+
Sbjct: 246 QKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLK--IGASGASILITTRLEKI 296

BLAST of Lag0022623 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 2.3e-35
Identity = 92/293 (31.40%), Postives = 153/293 (52.22%), Query Frame = 0

Query: 36  VQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHESVSMWVDNL 95
           +Q VL  +      ++ + +G + E  +L       +A+L D   K+L+ + +  W+  L
Sbjct: 6   IQVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKL 65

Query: 96  QDIVYEADDLLDELMAKQMKDITETLDRHYLKA------------SSLGVVGLVGEESTE 155
               YE DD+LDE   K  + +     R++ K               +  +  + EE  +
Sbjct: 66  NAATYEVDDILDEYKTKATRFLQSEYGRYHPKVIPFRHKVGKRMDQVMKKLNAIAEERKK 125

Query: 156 TNKNIDLMQ----FRETTSILNF-EVIGREVEASNIVKLVID-SSNQHLMSIIPIVGMGG 215
            +    +++     RET S+L   +V GR+ E   IVK++I+ +S+   +S++PI+GMGG
Sbjct: 126 FHLQEKIIERQAATRETGSVLTEPQVYGRDKEKDEIVKILINTASDAQKLSVLPILGMGG 185

Query: 216 IGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITNILEGIFQSLTNTSGGLKSKENLVR 275
           +GKTTL++++FN + V   F   IW+C+S+ F    +++ I +S+   S        L +
Sbjct: 186 LGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGKSLSDMDLAPLQK 245

Query: 276 GLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEIAGKSGNVIIVTTRSEKL 311
            LQ+ + GK YFLVLDDVWNE+   W  LR  LK   G SG  ++ TTR EK+
Sbjct: 246 KLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLK--VGASGAFVLTTTRLEKV 296

BLAST of Lag0022623 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.0e-35
Identity = 100/295 (33.90%), Postives = 151/295 (51.19%), Query Frame = 0

Query: 36  VQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHESVSMWVDNL 95
           +Q +L  +      ++ + +G + E  +L       +A+L D   K+L+++ +  W+  L
Sbjct: 6   IQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKL 65

Query: 96  QDIVYEADDLLDELMAKQMKDITETLDRHYLK--------ASSLGVVGLVGEESTETNKN 155
               YE DD+LDE   K  +       R++ K           +  V    +   E  KN
Sbjct: 66  NAATYEVDDILDEYKTKATRFSQSEYGRYHPKVIPFRHKVGKRMDQVMKKLKAIAEERKN 125

Query: 156 IDLMQ--------FRETTSILNF-EVIGREVEASNIVKLVID--SSNQHLMSIIPIVGMG 215
             L +         RET S+L   +V GR+ E   IVK++I+  S  QHL S++PI+GMG
Sbjct: 126 FHLHEKIVERQAVRRETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQHL-SVLPILGMG 185

Query: 216 GIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITNILEGIFQSLTNTS-GGLKSKENL 275
           G+GKTTLA+++FN + V  HF   IW+CVSE F    +++ I +S+      G      L
Sbjct: 186 GLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGRPLLGEMDLAPL 245

Query: 276 VRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEIAGKSGNVIIVTTRSEKL 311
            + LQ+ + GK Y LVLDDVWNE+   W  LR  LK   G SG  ++ TTR EK+
Sbjct: 246 QKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLK--VGASGASVLTTTRLEKV 297

BLAST of Lag0022623 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 5.7e-34
Identity = 94/302 (31.13%), Postives = 151/302 (50.00%), Query Frame = 0

Query: 36  VQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHESVSMWVDNL 95
           +Q +L+ +     +++ + +G EKE  KL       +A+L+D   K+L  +++  W+  L
Sbjct: 6   LQVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKL 65

Query: 96  QDIVYEADDLLDEL-------------------------MAKQMKDITETLDRHYLKASS 155
               YE DD+L E                          + ++MK+I E LD    +   
Sbjct: 66  NSAAYEVDDILGECKNEAIRFEQSRLGFYHPGIINFRHKIGRRMKEIMEKLDAISEERRK 125

Query: 156 LGVVGLVGEESTETNKNIDLMQFRETTSILNF-EVIGREVEASNIVKLVIDSSN-QHLMS 215
              +  + E              RET  +L   +V GR+ E   IVK++I++ N    + 
Sbjct: 126 FHFLEKITERQAAA-------ATRETGFVLTEPKVYGRDKEEDEIVKILINNVNVAEELP 185

Query: 216 IIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITNILEGIFQSLTNTSGG 275
           + PI+GMGG+GKTTLA++IFN E V  HF+  IWVCVS+ F    +++ I  ++  +S  
Sbjct: 186 VFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPH 245

Query: 276 LKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEIAGKSGNVIIVTTRSE 311
           ++   +  + LQ+ + GK Y LVLDDVWN++   W +LR  L    G  G  I+ TTR E
Sbjct: 246 VEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL--TVGARGASILATTRLE 298

BLAST of Lag0022623 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.4e-24
Identity = 79/286 (27.62%), Postives = 146/286 (51.05%), Query Frame = 0

Query: 58  EKELSKLREGLLHAEAILRDINRKRLHHESVSMWVDNLQDIVYEADDLLDELMAKQMK-- 117
           E  L +L   LL   A+L D   K++ +  V  WV+ L+D+VY A+D LD++  + ++  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 118 ---------------------DITETLDRHY---LKASSLGVVGLVGEESTETNKNIDLM 177
                                D  +    H    L+  ++ +  L  + +    K +  M
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155

Query: 178 ---QFRETTSILN-FEVIGREVEASNIVKLVI-DSSNQHLMSIIPIVGMGGIGKTTLAKV 237
              Q   TTS+++  EV GR+ +   I++ +I ++   + ++++ IVG+GG+GKTTL+++
Sbjct: 156 IPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQL 215

Query: 238 IFNHELVKGHFDKTIWVCVSEPFIITNILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGK 297
           ++N + V+ +F   +W  VSE F +  I + +++S+T+        + L   L++ + G 
Sbjct: 216 LYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLTGT 275

Query: 298 N--YFLVLDDVWNEEASLWGELRDCLKEIAGKSGNVIIVTTRSEKL 311
              + LVLDD+WNE  + W  LR     I    G+ I+VTTRS+++
Sbjct: 276 GLPFLLVLDDLWNENFADWDLLRQPF--IHAAQGSQILVTTRSQRV 319

BLAST of Lag0022623 vs. ExPASy TrEMBL
Match: A0A6J1CVR3 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014682 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.6e-111
Identity = 225/315 (71.43%), Postives = 247/315 (78.41%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGL+KELSKLR+ LL AE IL DINRK+L HE
Sbjct: 1   MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLDKELSKLRKRLLKAETILGDINRKKLRHE 60

Query: 87  SVSMWVDNLQDIVYEADDLLDEL---------------------------------MAKQ 146
           SV +WV++LQ +VY+ADDLLDEL                                 MA  
Sbjct: 61  SVRLWVEDLQHLVYQADDLLDELVYEDLRRKVEIEKINKVRNFFSLTNNPFLFRSKMANS 120

Query: 147 MKDITETLDRHYLKASSLGVVGLVGEESTET-NKNIDLMQFRETTSILNFEVIGREVEAS 206
           MK I+ETL +HY +AS+L   GLVGEE+TET NKNI L Q RETTSILNFEVIGRE+E  
Sbjct: 121 MKAISETLYQHYYEASAL---GLVGEETTETDNKNI-LKQIRETTSILNFEVIGREIEVL 180

Query: 207 NIVKLVIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIIT 266
           NIVKLVIDSS +H MSIIPIVGMGG+GKTTLAK++FNHEL+K HFDK+IWVCVSE FII 
Sbjct: 181 NIVKLVIDSSYEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKSIWVCVSESFIII 240

Query: 267 NILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKE 308
           NILEGIFQSLTNTS GL  KE L++ LQKEMQGK YFLVLDDVWNEE SLWGELRDCL E
Sbjct: 241 NILEGIFQSLTNTSSGLNYKETLLQRLQKEMQGKKYFLVLDDVWNEEVSLWGELRDCLNE 300

BLAST of Lag0022623 vs. ExPASy TrEMBL
Match: A0A6J1CVR6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014685 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 4.0e-107
Identity = 208/314 (66.24%), Postives = 244/314 (77.71%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAEFLWTFAVQEVL+K++K+AA+QIG+AWGLEKELSKL + LL AE IL DINR++LHH+
Sbjct: 1   MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHHD 60

Query: 87  SVSMWVDNLQDIVYEADDLLDEL---------------------------------MAKQ 146
           SV +W+++LQD+V+EA DLLDEL                                 MAK+
Sbjct: 61  SVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVEIGKMNKVRGSISRSYNLFSFRRKMAKK 120

Query: 147 MKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLMQFRETTSILNFEVIGREVEASN 206
           MKD+TETL +HY +AS L   GLVG+ES ETN+ I L Q RETTS LNFEV+GRE+E SN
Sbjct: 121 MKDVTETLYKHYCEASPL---GLVGDESIETNQ-IVLKQIRETTSNLNFEVVGREIEVSN 180

Query: 207 IVKLVIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITN 266
           IVKLVIDSSN+H  SIIPIVGMGG+GKTTLA++IFNHE++KGHFDKTIW+CVSEPFII N
Sbjct: 181 IVKLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIIIN 240

Query: 267 ILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEI 308
           ILEGIFQ LT TS GL SKE LV+ LQ+EM GK YFLVLDDVWNEE+ LW +L DCLK+I
Sbjct: 241 ILEGIFQGLTKTSSGLNSKETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQI 300

BLAST of Lag0022623 vs. ExPASy TrEMBL
Match: A0A6J1CUL8 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111014734 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 4.0e-107
Identity = 214/314 (68.15%), Postives = 239/314 (76.11%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAEFLWTFAVQEVLKKVLKLAAEQIGVAW L+KELSKLR+ LL AE IL DINR++L HE
Sbjct: 1   MAEFLWTFAVQEVLKKVLKLAAEQIGVAWALDKELSKLRKRLLKAETILGDINRRKLCHE 60

Query: 87  SVSMWVDNLQDIVYEADDLLDEL---------------------------------MAKQ 146
           SV +WV++LQ +VY+ADDLLD+L                                 MAK+
Sbjct: 61  SVRLWVEDLQHLVYQADDLLDDLVYEDLRQKVEMEKINKVRNFLSPSNNPFLFRFKMAKR 120

Query: 147 MKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLMQFRETTSILNFEVIGREVEASN 206
           MK I+ETL  HY +AS   V+G VGEESTET     L Q RETTSILNF+VIGRE+E   
Sbjct: 121 MKAISETLYEHYCEAS---VLGFVGEESTETTYKNILRQIRETTSILNFKVIGREIEVLK 180

Query: 207 IVKLVIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITN 266
           I+KLVIDSS++H MSIIPIVGMGG+GKTTLAK++FNHEL+K HFDKTIWVCVS+PFII N
Sbjct: 181 IMKLVIDSSHEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKTIWVCVSKPFIIVN 240

Query: 267 ILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEI 308
           ILEGIFQSLTNTS GL  KE L   LQKEMQGK YFLVLDDVWNEE +LW ELRDCL EI
Sbjct: 241 ILEGIFQSLTNTSSGLNYKETLFHRLQKEMQGKKYFLVLDDVWNEEVALWDELRDCLNEI 300

BLAST of Lag0022623 vs. ExPASy TrEMBL
Match: A0A6J1CU86 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014619 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 2.8e-100
Identity = 201/314 (64.01%), Postives = 235/314 (74.84%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAEFLWTFAVQEVL+K++K+AA+QIG+A GLEKELS L + L  AE ILRDINRK+ H +
Sbjct: 1   MAEFLWTFAVQEVLRKIVKVAAQQIGLARGLEKELSNLTKWLQKAETILRDINRKKSHSD 60

Query: 87  SVSMWVDNLQDIVYEADDLLDEL---------------------------------MAKQ 146
           SV +WV++LQD+V+EADDLLDEL                                 MAK+
Sbjct: 61  SVRLWVEDLQDVVHEADDLLDELVYEHLRRKVEIGKINKVRDSFSTSHNVFLFRLKMAKK 120

Query: 147 MKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLMQFRETTSILNFEVIGREVEASN 206
           +KDIT+ L +HY +AS L   GLVG+ESTE N+ I L Q R+TTS LNFEV+GRE E SN
Sbjct: 121 IKDITQILHKHYCEASPL---GLVGQESTEANQ-IVLSQIRDTTSNLNFEVVGREDEVSN 180

Query: 207 IVKLVIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITN 266
           IVKLVIDSSN+H MSI+PIVGMGG+GKTT A+ IFNH+ +KGHFD  IWVCVSEPFII N
Sbjct: 181 IVKLVIDSSNEHHMSILPIVGMGGLGKTTFAQKIFNHDTIKGHFDNIIWVCVSEPFIIIN 240

Query: 267 ILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEI 308
           ILEGIFQSLT TS GL  K  L+  +QKEM GK YFLVLDDVWNEE+ LW +L DCLK+I
Sbjct: 241 ILEGIFQSLTKTSSGLNHKGVLLEKIQKEMHGKMYFLVLDDVWNEESGLWDDLGDCLKQI 300

BLAST of Lag0022623 vs. ExPASy TrEMBL
Match: A0A0A0KST5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 2.4e-91
Identity = 186/315 (59.05%), Postives = 223/315 (70.79%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAEFLWTFAVQEVLKK++   AEQI +AWGLEKELS L++ LL A+ IL DIN K+ HH 
Sbjct: 1   MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHHH 60

Query: 87  SVSMWVDNLQDIVYEADDLLDEL----------------------------------MAK 146
           SV +WV+ L DI+YEADDLLDE+                                  MAK
Sbjct: 61  SVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLRKVRDSISPSKNSFLFGLKMAK 120

Query: 147 QMKDITETLDRHYLKASSLGVVGLVGEESTETNKNIDLMQFRETTSILNFEVIGREVEAS 206
           +MK IT+TL  HY +AS L   GLVG+EST T     L Q RETTSIL+FEV GRE E  
Sbjct: 121 KMKKITKTLYEHYCEASPL---GLVGDEST-TESEAALNQIRETTSILDFEVEGREAEVL 180

Query: 207 NIVKLVIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIIT 266
            I+KLVIDS+++  +S+I IVGMGG+GKTTLAK++FNH+ +KGHFDKT+WVCVS+PFI+ 
Sbjct: 181 EILKLVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVM 240

Query: 267 NILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKE 308
            ILE IFQ LTNTS GL S+E L+  L++EMQGK YFLVLDDVW++E  LW EL   LK 
Sbjct: 241 KILEAIFQGLTNTSSGLNSREALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKY 300

BLAST of Lag0022623 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 113.2 bits (282), Expect = 3.8e-25
Identity = 79/286 (27.62%), Postives = 146/286 (51.05%), Query Frame = 0

Query: 58  EKELSKLREGLLHAEAILRDINRKRLHHESVSMWVDNLQDIVYEADDLLDELMAKQMK-- 117
           E  L +L   LL   A+L D   K++ +  V  WV+ L+D+VY A+D LD++  + ++  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 118 ---------------------DITETLDRHY---LKASSLGVVGLVGEESTETNKNIDLM 177
                                D  +    H    L+  ++ +  L  + +    K +  M
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGLKELTAM 155

Query: 178 ---QFRETTSILN-FEVIGREVEASNIVKLVI-DSSNQHLMSIIPIVGMGGIGKTTLAKV 237
              Q   TTS+++  EV GR+ +   I++ +I ++   + ++++ IVG+GG+GKTTL+++
Sbjct: 156 IPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTLSQL 215

Query: 238 IFNHELVKGHFDKTIWVCVSEPFIITNILEGIFQSLTNTSGGLKSKENLVRGLQKEMQGK 297
           ++N + V+ +F   +W  VSE F +  I + +++S+T+        + L   L++ + G 
Sbjct: 216 LYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERLTGT 275

Query: 298 N--YFLVLDDVWNEEASLWGELRDCLKEIAGKSGNVIIVTTRSEKL 311
              + LVLDD+WNE  + W  LR     I    G+ I+VTTRS+++
Sbjct: 276 GLPFLLVLDDLWNENFADWDLLRQPF--IHAAQGSQILVTTRSQRV 319

BLAST of Lag0022623 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 110.9 bits (276), Expect = 1.9e-24
Identity = 86/297 (28.96%), Postives = 150/297 (50.51%), Query Frame = 0

Query: 40  LKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHESVSMWVDNLQDIV 99
           L+K L +  E+        K+L  L+  L + ++ L+D  R++  +E++   V +L+++V
Sbjct: 10  LEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELV 69

Query: 100 YEADDLLDELMAKQMKDITE--------------TLDRHYLKASSL-----------GVV 159
           YEA+D+L +       D  E               +   Y K+  L             V
Sbjct: 70  YEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPARVPLQYKKSKRLQEINERITKIKSQV 129

Query: 160 GLVGEESTETNKNIDLMQFRETTSILNF-EVIGREVEASNIVKLVIDSSNQHLMSIIPIV 219
               E  T +N   D    R ++ + +  +V+G E +   I + +  S++  L+ I+  V
Sbjct: 130 EPYFEFITPSNVGRDNGTDRWSSPVYDHTQVVGLEGDKRKIKEWLFRSNDSQLL-IMAFV 189

Query: 220 GMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITNILEGIFQSLTNTSGGLKSKE 279
           GMGG+GKTT+A+ +FN + ++  F++ IWV VS+ F    I+  I ++L + S G     
Sbjct: 190 GMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVG-DDIG 249

Query: 280 NLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEIAGKSGNVIIVTTRSEKL 311
            L+R +Q+ + GK Y +V+DDVW++  S W ++   L    G+ G+V IVTTRSE +
Sbjct: 250 TLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPR--GQGGSV-IVTTRSESV 301

BLAST of Lag0022623 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 110.9 bits (276), Expect = 1.9e-24
Identity = 86/297 (28.96%), Postives = 150/297 (50.51%), Query Frame = 0

Query: 40  LKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHESVSMWVDNLQDIV 99
           L+K L +  E+        K+L  L+  L + ++ L+D  R++  +E++   V +L+++V
Sbjct: 10  LEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELV 69

Query: 100 YEADDLLDELMAKQMKDITE--------------TLDRHYLKASSL-----------GVV 159
           YEA+D+L +       D  E               +   Y K+  L             V
Sbjct: 70  YEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPARVPLQYKKSKRLQEINERITKIKSQV 129

Query: 160 GLVGEESTETNKNIDLMQFRETTSILNF-EVIGREVEASNIVKLVIDSSNQHLMSIIPIV 219
               E  T +N   D    R ++ + +  +V+G E +   I + +  S++  L+ I+  V
Sbjct: 130 EPYFEFITPSNVGRDNGTDRWSSPVYDHTQVVGLEGDKRKIKEWLFRSNDSQLL-IMAFV 189

Query: 220 GMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITNILEGIFQSLTNTSGGLKSKE 279
           GMGG+GKTT+A+ +FN + ++  F++ IWV VS+ F    I+  I ++L + S G     
Sbjct: 190 GMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVG-DDIG 249

Query: 280 NLVRGLQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEIAGKSGNVIIVTTRSEKL 311
            L+R +Q+ + GK Y +V+DDVW++  S W ++   L    G+ G+V IVTTRSE +
Sbjct: 250 TLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPR--GQGGSV-IVTTRSESV 301

BLAST of Lag0022623 vs. TAIR 10
Match: AT1G53350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 110.2 bits (274), Expect = 3.2e-24
Identity = 85/317 (26.81%), Postives = 161/317 (50.79%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAE + +F V+    K+ +L + +     G+++++  L+  L   +++L+D + K+   E
Sbjct: 1   MAEAVVSFGVE----KLWELLSRESARLNGIDEQVDGLKRQLGRLQSLLKDADAKKNETE 60

Query: 87  SVSMWVDNLQDIVYEADD-----LLDELMAKQ-----------------------MKDIT 146
            V  ++++++DIVY+ADD     LL+EL  K+                       ++ IT
Sbjct: 61  RVRNFLEDVKDIVYDADDIIESFLLNELRGKEKGIKKQVRTLACFLVDRRKFASDIEGIT 120

Query: 147 ETLDRHYLKASSLGVVGLVGEESTETNKNIDLMQFRETTS-ILNFEVIGREVEASNIVKL 206
           + +    +   SLG+  +        +      + R+T S     +++G +     +V  
Sbjct: 121 KRISEVIVGMQSLGIQHIADGGGRSLSLQERQREIRQTFSRNSESDLVGLDQSVEELVDH 180

Query: 207 VIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITNILEG 266
           ++++ +   + ++ + GMGGIGKTTLA+ +F+H++V+ HFD   WVCVS+ F   ++ + 
Sbjct: 181 LVENDS---VQVVSVSGMGGIGKTTLARQVFHHDIVRRHFDGFSWVCVSQQFTRKDVWQR 240

Query: 267 IFQSLTNTSGG-LKSKENLVRG-LQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEIAG 313
           I Q L     G ++  E  ++G L + ++   Y LVLDDVW EE   W  ++        
Sbjct: 241 ILQDLRPYDEGIIQMDEYTLQGELFELLESGRYLLVLDDVWKEED--WDRIKAVFPH--- 300

BLAST of Lag0022623 vs. TAIR 10
Match: AT5G35450.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 107.1 bits (266), Expect = 2.7e-23
Identity = 86/317 (27.13%), Postives = 169/317 (53.31%), Query Frame = 0

Query: 27  MAEFLWTFAVQEVLKKVLKLAAEQIGVAWGLEKELSKLREGLLHAEAILRDINRKRLHHE 86
           MAE + +F VQ+ L  +L   +E++    G+++++  L+  L   +++L+D + K+   +
Sbjct: 1   MAEGVVSFGVQK-LWALLNRESERLN---GIDEQVDGLKRQLRGLQSLLKDADAKKHGSD 60

Query: 87  SVSMWVDNLQDIVYEADDLLDELM-------AKQMKDITETL-----DRHYLKASSLGVV 146
            V  ++++++D+V++A+D+++  +        K +K+    L     DRH + +   G+ 
Sbjct: 61  RVRNFLEDVKDLVFDAEDIIESYVLNKLRGEGKGVKNHVRRLACFLTDRHKVASDIEGIT 120

Query: 147 ----GLVGE--------ESTETNKNIDLM----QFRET-TSILNFEVIGREVEASNIVKL 206
                ++GE        +  +  +++ L     + R+T  +    +++G E     +V  
Sbjct: 121 KRISKVIGEMQSLGIQQQIIDGGRSLSLQDIQREIRQTFPNSSESDLVGVEQSVEELVGP 180

Query: 207 VIDSSNQHLMSIIPIVGMGGIGKTTLAKVIFNHELVKGHFDKTIWVCVSEPFIITNILEG 266
           +++  N   + ++ I GMGGIGKTTLA+ IF+H+LV+ HFD   WVCVS+ F   ++ + 
Sbjct: 181 MVEIDN---IQVVSISGMGGIGKTTLARQIFHHDLVRRHFDGFAWVCVSQQFTQKHVWQR 240

Query: 267 IFQSLTNTSGG-LKSKENLVRG-LQKEMQGKNYFLVLDDVWNEEASLWGELRDCLKEIAG 313
           I Q L    G  L+  E  ++G L + ++   Y +VLDDVW EE   W  +++       
Sbjct: 241 ILQELRPHDGEILQMDEYTIQGKLFQLLETGRYLVVLDDVWKEED--WDRIKEVFPR--- 300