Lag0020061 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020061
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein of unknown function (DUF3464)
Locationchr5: 47776403 .. 47776975 (-)
RNA-Seq ExpressionLag0020061
SyntenyLag0020061
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACTCTGGTGTGTTCCTTTCGTCAAGCTCTCGACCTCTCGAAGCCTCCGCCATGGAAACAGAGAAGCCGAATCAACGACTCAACACTCACAAAGAATCTCAGCAATTTCACGATAACATCCAGACGCCAAATCCAACTAAACGCCAAGGGCTTCACAGGTTCTTCGGCGACGGCGAAAGACAGAGAAACCGCCGCGGAAAAACTTTCAGGCCAAAACAGTAGCAAAGATGACGGGGGCGACTACGACGATGAAATTCCAGAGGCCGTAGCCAAGAGGATCATAACGAGAATCTTGGCTTTCGTCGGAATTCCGATGGCGTTCGGTGTGACGTTGCTGAAGATTTTCGAGGCGATCAAGGTGCAGAACCTGTGGGATGTGCCGATCTGGCTGCCATTTTTGACGATTTTTTTGACATTTGGAGCTTCGACGATGGGAATTGCTTACGGGACGTTATCGGCGAGTTTGGATCCGGAGAAGGAAGGGACTCTGTTTGGATGGGAACAGGTGGAGAAGAATTGGGTAGAGATGTGGAAGGAGGAAGATGAAGCCAATCGCCGAGGAAACTGA

mRNA sequence

ATGAAAACTCTGGTGTGTTCCTTTCGTCAAGCTCTCGACCTCTCGAAGCCTCCGCCATGGAAACAGAGAAGCCGAATCAACGACTCAACACTCACAAAGAATCTCAGCAATTTCACGATAACATCCAGACGCCAAATCCAACTAAACGCCAAGGGCTTCACAGGTTCTTCGGCGACGGCGAAAGACAGAGAAACCGCCGCGGAAAAACTTTCAGGCCAAAACAGTAGCAAAGATGACGGGGGCGACTACGACGATGAAATTCCAGAGGCCGTAGCCAAGAGGATCATAACGAGAATCTTGGCTTTCGTCGGAATTCCGATGGCGTTCGGTGTGACGTTGCTGAAGATTTTCGAGGCGATCAAGGTGCAGAACCTGTGGGATGTGCCGATCTGGCTGCCATTTTTGACGATTTTTTTGACATTTGGAGCTTCGACGATGGGAATTGCTTACGGGACGTTATCGGCGAGTTTGGATCCGGAGAAGGAAGGGACTCTGTTTGGATGGGAACAGGTGGAGAAGAATTGGGTAGAGATGTGGAAGGAGGAAGATGAAGCCAATCGCCGAGGAAACTGA

Coding sequence (CDS)

ATGAAAACTCTGGTGTGTTCCTTTCGTCAAGCTCTCGACCTCTCGAAGCCTCCGCCATGGAAACAGAGAAGCCGAATCAACGACTCAACACTCACAAAGAATCTCAGCAATTTCACGATAACATCCAGACGCCAAATCCAACTAAACGCCAAGGGCTTCACAGGTTCTTCGGCGACGGCGAAAGACAGAGAAACCGCCGCGGAAAAACTTTCAGGCCAAAACAGTAGCAAAGATGACGGGGGCGACTACGACGATGAAATTCCAGAGGCCGTAGCCAAGAGGATCATAACGAGAATCTTGGCTTTCGTCGGAATTCCGATGGCGTTCGGTGTGACGTTGCTGAAGATTTTCGAGGCGATCAAGGTGCAGAACCTGTGGGATGTGCCGATCTGGCTGCCATTTTTGACGATTTTTTTGACATTTGGAGCTTCGACGATGGGAATTGCTTACGGGACGTTATCGGCGAGTTTGGATCCGGAGAAGGAAGGGACTCTGTTTGGATGGGAACAGGTGGAGAAGAATTGGGTAGAGATGTGGAAGGAGGAAGATGAAGCCAATCGCCGAGGAAACTGA

Protein sequence

MKTLVCSFRQALDLSKPPPWKQRSRINDSTLTKNLSNFTITSRRQIQLNAKGFTGSSATAKDRETAAEKLSGQNSSKDDGGDYDDEIPEAVAKRIITRILAFVGIPMAFGVTLLKIFEAIKVQNLWDVPIWLPFLTIFLTFGASTMGIAYGTLSASLDPEKEGTLFGWEQVEKNWVEMWKEEDEANRRGN
Homology
BLAST of Lag0020061 vs. NCBI nr
Match: XP_023541387.1 (uncharacterized protein PAM68-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 311.6 bits (797), Expect = 4.6e-81
Identity = 158/190 (83.16%), Postives = 168/190 (88.42%), Query Frame = 0

Query: 1   MKTLVCSFRQALDLSKPPPWKQRSRINDSTLTKNLSNFTITSRRQIQLNAKGFTGSSATA 60
           MK LVCSFR ALDLSKPPPWKQRSRI+DST TKNL  FT TSR QIQLNAKGFTGS ATA
Sbjct: 1   MKALVCSFRHALDLSKPPPWKQRSRIDDSTATKNLRIFTRTSRGQIQLNAKGFTGSPATA 60

Query: 61  KDRETAAEKLSGQNSSKDDGGDYDDEIPEAVAKRIITRILAFVGIPMAFGVTLLKIFEAI 120
           KDRETA +K SGQN+S+  GG  DDEIPEAVAKRIITRILAFVG+PMAFGVTL+KI EA+
Sbjct: 61  KDRETAVKKPSGQNNSRGGGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKILEAV 120

Query: 121 KVQNLWDVPIWLPFLTIFLTFGASTMGIAYGTLSASLDPEKEGTLFGWEQVEKNWVEMWK 180
             Q LW VPIWLPFLTIFLTFGASTMGIAYGTLS SLDPEKEGTLFGW++ +KNWVEMWK
Sbjct: 121 HEQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWK 180

Query: 181 EEDEANRRGN 191
           EEDEANR GN
Sbjct: 181 EEDEANRGGN 190

BLAST of Lag0020061 vs. NCBI nr
Match: KAG7013265.1 (putative protein PAM68-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 311.2 bits (796), Expect = 6.0e-81
Identity = 160/190 (84.21%), Postives = 169/190 (88.95%), Query Frame = 0

Query: 1   MKTLVCSFRQALDLSKPPPWKQRSRINDSTLTKNLSNFTITSRRQIQLNAKGFTGSSATA 60
           MK LVCSFR ALDLSKPPPWKQRSRI+DST TKNL  FT TSR QIQLNAKGFTGS ATA
Sbjct: 1   MKALVCSFRHALDLSKPPPWKQRSRIDDSTATKNLRIFTRTSRGQIQLNAKGFTGSPATA 60

Query: 61  KDRETAAEKLSGQNSSKDDGGDYDDEIPEAVAKRIITRILAFVGIPMAFGVTLLKIFEAI 120
           KDRETA +K SGQN+S+D GG  DDEIPEAVAKRIITRILAFVGIPMAFGVTL+KI EA+
Sbjct: 61  KDRETAVKKPSGQNNSRDGGGG-DDEIPEAVAKRIITRILAFVGIPMAFGVTLVKILEAV 120

Query: 121 KVQNLWDVPIWLPFLTIFLTFGASTMGIAYGTLSASLDPEKEGTLFGWEQVEKNWVEMWK 180
             Q LW VPIWLPFLTIFLTFGASTMGIAYGTLS SLDPEKEGTLFGW++ +KNWVEMWK
Sbjct: 121 HEQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWK 180

Query: 181 EEDEANRRGN 191
           EEDEANR GN
Sbjct: 181 EEDEANRGGN 189

BLAST of Lag0020061 vs. NCBI nr
Match: XP_022944901.1 (uncharacterized protein PAM68-like [Cucurbita moschata])

HSP 1 Score: 310.8 bits (795), Expect = 7.9e-81
Identity = 159/190 (83.68%), Postives = 169/190 (88.95%), Query Frame = 0

Query: 1   MKTLVCSFRQALDLSKPPPWKQRSRINDSTLTKNLSNFTITSRRQIQLNAKGFTGSSATA 60
           MK LVCSFR AL LSKPPPWKQRSRI+DST TKNL  FT TSR QIQLNAKGFTGS ATA
Sbjct: 1   MKALVCSFRHALHLSKPPPWKQRSRIDDSTTTKNLRIFTRTSRGQIQLNAKGFTGSPATA 60

Query: 61  KDRETAAEKLSGQNSSKDDGGDYDDEIPEAVAKRIITRILAFVGIPMAFGVTLLKIFEAI 120
           KDRETA +K SGQN+S+D GGD DDEIPEAVAKRIITRILAFVG+PMAFGVTL+KI EA+
Sbjct: 61  KDRETAVKKPSGQNNSRDGGGD-DDEIPEAVAKRIITRILAFVGVPMAFGVTLVKILEAV 120

Query: 121 KVQNLWDVPIWLPFLTIFLTFGASTMGIAYGTLSASLDPEKEGTLFGWEQVEKNWVEMWK 180
             Q LW VPIWLPFLTIFLTFGASTMGIAYGTLS SLDPEKEGTLFGW++ +KNWVEMWK
Sbjct: 121 HEQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWK 180

Query: 181 EEDEANRRGN 191
           EEDEANR GN
Sbjct: 181 EEDEANRGGN 189

BLAST of Lag0020061 vs. NCBI nr
Match: KAG6574203.1 (putative protein PAM68-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 310.1 bits (793), Expect = 1.3e-80
Identity = 160/191 (83.77%), Postives = 169/191 (88.48%), Query Frame = 0

Query: 1   MKTLVCSFRQALDLSKPPPWKQRSRINDSTLTKNLSNFTITSRRQIQLNAKGFTGSSATA 60
           MK LVCSFR AL LSKPPPWKQRSRI+DST TKNL  FT TSR QIQLNAKGFTGS ATA
Sbjct: 1   MKALVCSFRHALHLSKPPPWKQRSRIDDSTATKNLRIFTRTSRGQIQLNAKGFTGSPATA 60

Query: 61  KDRETAAEKLSGQNSSKD-DGGDYDDEIPEAVAKRIITRILAFVGIPMAFGVTLLKIFEA 120
           KDRETA +K SGQN+S+D  GGD DDEIPEAVAKRIITRILAFVGIPMAFGVTL+KI EA
Sbjct: 61  KDRETAVKKPSGQNNSRDGGGGDDDDEIPEAVAKRIITRILAFVGIPMAFGVTLVKILEA 120

Query: 121 IKVQNLWDVPIWLPFLTIFLTFGASTMGIAYGTLSASLDPEKEGTLFGWEQVEKNWVEMW 180
           +  Q LW VPIWLPFLTIFLTFGASTMGIAYGTLS SLDPEKEGTLFGW++ +KNWVEMW
Sbjct: 121 VHEQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMW 180

Query: 181 KEEDEANRRGN 191
           KEEDEANR GN
Sbjct: 181 KEEDEANRGGN 191

BLAST of Lag0020061 vs. NCBI nr
Match: XP_022968628.1 (uncharacterized protein PAM68-like [Cucurbita maxima])

HSP 1 Score: 305.8 bits (782), Expect = 2.5e-79
Identity = 159/190 (83.68%), Postives = 170/190 (89.47%), Query Frame = 0

Query: 1   MKTLVCSFRQALDLSKPPPWKQRSRINDSTLTKNLSNFTITSRRQIQLNAKGFTGSSATA 60
           MK LVCSFR ALDLSKPPPWKQRSRI++ST TKNL  FT TSR QIQLNAKGFTGS ATA
Sbjct: 1   MKALVCSFRHALDLSKPPPWKQRSRIDNSTTTKNLRVFTGTSRGQIQLNAKGFTGSPATA 60

Query: 61  KDRETAAEKLSGQNSSKDDGGDYDDEIPEAVAKRIITRILAFVGIPMAFGVTLLKIFEAI 120
           KDRETA +KLS QN+S+ DGGD DDEIPEAVAKRIITRILAFVGIPMAFGVTL+KI EA+
Sbjct: 61  KDRETAVKKLSDQNNSR-DGGD-DDEIPEAVAKRIITRILAFVGIPMAFGVTLVKILEAV 120

Query: 121 KVQNLWDVPIWLPFLTIFLTFGASTMGIAYGTLSASLDPEKEGTLFGWEQVEKNWVEMWK 180
             Q LW VPIWLPFLTIFLTFGASTMGIAYGTLS SLDPEKEGTLFGW++ +KNWVEMWK
Sbjct: 121 HEQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWK 180

Query: 181 EEDEANRRGN 191
           EEDEANR G+
Sbjct: 181 EEDEANRGGD 188

BLAST of Lag0020061 vs. ExPASy Swiss-Prot
Match: Q9LTD9 (Uncharacterized protein PAM68-like OS=Arabidopsis thaliana OX=3702 GN=At5g52780 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.7e-25
Identity = 67/182 (36.81%), Postives = 102/182 (56.04%), Query Frame = 0

Query: 1   MKTLVCSFRQALDLSKPPPWKQRSRINDSTLTKNLSNFTITSRRQIQLNAKGFTGSSATA 60
           M+ L+CS R         P    SR    T + N         + +  N K    S   A
Sbjct: 1   MRALLCSHRLL-------PLSSLSRTTVKTKSHN--------PKTLYPNNKPRWESKLHA 60

Query: 61  KDRETAAEKLSGQNSSKDDGGDYDDEIPEAVAKRIITRILAFVGIPMAFGVTLLKIFEAI 120
             +   + + S +    D   + D  IP+ V +R++ RI+  VG P+  GV +LK+ E +
Sbjct: 61  GPKGFQSSRTSEKPGRPDPDPEDDPPIPQEVFERMMGRIVVSVGTPLGLGVAILKVLEVL 120

Query: 121 KVQNLWDVPIWLPFLTIFLTFGASTMGIAYGTLSASLDPEKEGTLFGWEQVEKNWVEMWK 180
           K +N+WDVP+W+P+LT  +TFG+S +GIAYG+LS +LDP K  +LFG ++ ++NWVEMWK
Sbjct: 121 KDRNVWDVPLWVPYLTTLVTFGSSALGIAYGSLSTNLDPAKTNSLFGLKEAKENWVEMWK 167

Query: 181 EE 183
           E+
Sbjct: 181 ED 167

BLAST of Lag0020061 vs. ExPASy Swiss-Prot
Match: O49668 (Protein PAM68, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM68 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.9e-17
Identity = 57/175 (32.57%), Postives = 88/175 (50.29%), Query Frame = 0

Query: 25  RINDSTLTKNLSNFTITSRRQIQLNA---------KGFTGSSATAKDRETAAEKLSGQNS 84
           R+ D T ++   + T  +R +I   A         KGF       K +++   K   Q+ 
Sbjct: 33  RLRDKTKSQVPKSITCINRLEISRIAPLHATMNSPKGF--GPPPKKTKKSKKPKPGNQSD 92

Query: 85  SKDDGGDYDDE----------IPEAVAKRIITRILAFVGIPMAFGVTLLKIFEAIKVQNL 144
             DD  D DD+          IPE V  R+I+R+   VG+P+  G+     F  +KV   
Sbjct: 93  EDDDDEDEDDDDEEDERERGVIPEIVTNRMISRMGFTVGLPLFIGLLFFPFFYYLKVGLK 152

Query: 145 WDVPIWLPFLTIFLTFGASTMGIAYGTLSASLDPEKEGTLFGWEQVEKNWVEMWK 181
            DVP W+PF+  F+ FG +  G++YG +S+S DP +EG+L GW + +KNW   W+
Sbjct: 153 VDVPTWVPFIVSFVFFGTALAGVSYGIVSSSWDPLREGSLLGWNEAKKNWPVFWQ 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023541387.14.6e-8183.16uncharacterized protein PAM68-like [Cucurbita pepo subsp. pepo][more]
KAG7013265.16.0e-8184.21putative protein PAM68-like protein, partial [Cucurbita argyrosperma subsp. argy... [more]
XP_022944901.17.9e-8183.68uncharacterized protein PAM68-like [Cucurbita moschata][more]
KAG6574203.11.3e-8083.77putative protein PAM68-like protein, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022968628.12.5e-7983.68uncharacterized protein PAM68-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LTD93.7e-2536.81Uncharacterized protein PAM68-like OS=Arabidopsis thaliana OX=3702 GN=At5g52780 ... [more]
O496682.9e-1732.57Protein PAM68, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM68 PE=1 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021855PAM68-likePFAMPF11947DUF3464coord: 60..181
e-value: 1.8E-38
score: 131.6
IPR021855PAM68-likePANTHERPTHR34575PROTEIN PAM68, CHLOROPLASTICcoord: 1..186
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 57..83
NoneNo IPR availablePANTHERPTHR34575:SF6EXPRESSED PROTEINcoord: 1..186

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020061.1Lag0020061.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane