Lag0011782 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0011782
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptiontRNA-uridine aminocarboxypropyltransferase
Locationchr1: 32813211 .. 32813975 (+)
RNA-Seq ExpressionLag0011782
SyntenyLag0011782
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCCAACAAACCACCACCACCGCAACTCCACCGCCGCAGCCACCGGAACTTCGTCCTCCTCACCGCCACCACGGCGAACAATTTGCCCTAATTGCACTCGTCCCCAACCCGTCTGCCTCTGCAAATTCCTTCCATCTCCACCAATCGCAACCAAAACACAGATCATCATCCTCCAACATCCTCACGAGGCACAACACAAGCTCTCCACCACACCGATCCTCACCAAATGCCTCCAAAACGCAACCACCATCGTCGGTCGCCGTCTGAAGCCAGGCCTCTCCGAACTCCTCGACCGATCCCCTCCCGCCGTGTTCCTCTTCCCGCCGACGCCTCACTCGTCGCCGCCGACCAACATATCGGAGCTCGACCTAGCATCCTCGGACTCGGATCTGGTCTTGATAGCGTTCGACGGGACGTGGAAGCACGCGAAGGAGATGGTGCGATCGAGCGAGGAGTTCCTGTCGAAGTTCGCGAGGAGAGTTTGCTTGGACTTCGACGACGGCGTGGACGGCGGAAGCATCTTCGATTCGGAGTTGATTCTGAGGAAGGAGCCGCATGGCGGATGCGTTAGCACGATGGAGGCGGTGGCGAGGTGCTTGAGAGTGATGGAGTCGGAGGGGGCGGAGATTGAGAGGAGATTGATTGGAGTTTTGAGGGAAATGGTTAGGTTACAGGCTGCGTTCTTGAAGCCCATGAACCCTAGGCCCAAATTGTTGAAGATGAAAAAGATCATGAAGAAGAAGAAGGAAAGCGAGGATTAA

mRNA sequence

ATGGACCCAACAAACCACCACCACCGCAACTCCACCGCCGCAGCCACCGGAACTTCGTCCTCCTCACCGCCACCACGGCGAACAATTTGCCCTAATTGCACTCGTCCCCAACCCGTCTGCCTCTGCAAATTCCTTCCATCTCCACCAATCGCAACCAAAACACAGATCATCATCCTCCAACATCCTCACGAGGCACAACACAAGCTCTCCACCACACCGATCCTCACCAAATGCCTCCAAAACGCAACCACCATCGTCGGTCGCCGTCTGAAGCCAGGCCTCTCCGAACTCCTCGACCGATCCCCTCCCGCCGTGTTCCTCTTCCCGCCGACGCCTCACTCGTCGCCGCCGACCAACATATCGGAGCTCGACCTAGCATCCTCGGACTCGGATCTGGTCTTGATAGCGTTCGACGGGACGTGGAAGCACGCGAAGGAGATGGTGCGATCGAGCGAGGAGTTCCTGTCGAAGTTCGCGAGGAGAGTTTGCTTGGACTTCGACGACGGCGTGGACGGCGGAAGCATCTTCGATTCGGAGTTGATTCTGAGGAAGGAGCCGCATGGCGGATGCGTTAGCACGATGGAGGCGGTGGCGAGGTGCTTGAGAGTGATGGAGTCGGAGGGGGCGGAGATTGAGAGGAGATTGATTGGAGTTTTGAGGGAAATGGTTAGGTTACAGGCTGCGTTCTTGAAGCCCATGAACCCTAGGCCCAAATTGTTGAAGATGAAAAAGATCATGAAGAAGAAGAAGGAAAGCGAGGATTAA

Coding sequence (CDS)

ATGGACCCAACAAACCACCACCACCGCAACTCCACCGCCGCAGCCACCGGAACTTCGTCCTCCTCACCGCCACCACGGCGAACAATTTGCCCTAATTGCACTCGTCCCCAACCCGTCTGCCTCTGCAAATTCCTTCCATCTCCACCAATCGCAACCAAAACACAGATCATCATCCTCCAACATCCTCACGAGGCACAACACAAGCTCTCCACCACACCGATCCTCACCAAATGCCTCCAAAACGCAACCACCATCGTCGGTCGCCGTCTGAAGCCAGGCCTCTCCGAACTCCTCGACCGATCCCCTCCCGCCGTGTTCCTCTTCCCGCCGACGCCTCACTCGTCGCCGCCGACCAACATATCGGAGCTCGACCTAGCATCCTCGGACTCGGATCTGGTCTTGATAGCGTTCGACGGGACGTGGAAGCACGCGAAGGAGATGGTGCGATCGAGCGAGGAGTTCCTGTCGAAGTTCGCGAGGAGAGTTTGCTTGGACTTCGACGACGGCGTGGACGGCGGAAGCATCTTCGATTCGGAGTTGATTCTGAGGAAGGAGCCGCATGGCGGATGCGTTAGCACGATGGAGGCGGTGGCGAGGTGCTTGAGAGTGATGGAGTCGGAGGGGGCGGAGATTGAGAGGAGATTGATTGGAGTTTTGAGGGAAATGGTTAGGTTACAGGCTGCGTTCTTGAAGCCCATGAACCCTAGGCCCAAATTGTTGAAGATGAAAAAGATCATGAAGAAGAAGAAGGAAAGCGAGGATTAA

Protein sequence

MDPTNHHHRNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQHPHEAQHKLSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNISELDLASSDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFDSELILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRPKLLKMKKIMKKKKESED
Homology
BLAST of Lag0011782 vs. NCBI nr
Match: XP_023544253.1 (DTW domain-containing protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 429.9 bits (1104), Expect = 1.6e-116
Identity = 220/257 (85.60%), Postives = 235/257 (91.44%), Query Frame = 0

Query: 1   MDPTNHHHRNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60
           M+PTNHHH  ++AA   +++SSPP RR+ICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ
Sbjct: 1   MEPTNHHH--NSAAGYSSAASSPPARRSICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60

Query: 61  HPHEAQHKLSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNI 120
           HPHEAQHKLSTTPILTKCL NATTIV RRLKPGLS+LLDRSP AV+LFPPTPHSSPP NI
Sbjct: 61  HPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSSPPINI 120

Query: 121 SELDLASS---DSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFD 180
           SELDL SS   DS+LVLIAFDGTWKHAKEMVRSSEEFLS+FARR+CLDFD+ VDGGSIFD
Sbjct: 121 SELDLTSSSSPDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVDGGSIFD 180

Query: 181 SELILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRP 240
           SELILRKEPHGGCVSTMEAVARCLRVME++GAEIE RLIGVLREMVRLQA FLKPMNPRP
Sbjct: 181 SELILRKEPHGGCVSTMEAVARCLRVMEADGAEIEVRLIGVLREMVRLQAGFLKPMNPRP 240

Query: 241 KLLKMKKIMKKKKESED 255
           KLLK KK MK K E ED
Sbjct: 241 KLLKTKKKMKNKSERED 255

BLAST of Lag0011782 vs. NCBI nr
Match: XP_022928716.1 (DTW domain-containing protein 2 [Cucurbita moschata])

HSP 1 Score: 422.9 bits (1086), Expect = 1.9e-114
Identity = 217/257 (84.44%), Postives = 230/257 (89.49%), Query Frame = 0

Query: 1   MDPTNHHHRNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60
           M+PTNHHH         +++SSPP RR+ICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ
Sbjct: 1   MEPTNHHH--------NSAASSPPARRSICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60

Query: 61  HPHEAQHKLSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNI 120
           HPHEAQHKLSTTPILTKCL NATTIV RRLKPGLS+LLDRSP AV+LFPPTPHSSPP NI
Sbjct: 61  HPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSSPPINI 120

Query: 121 SELDLASS---DSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFD 180
           SELDL SS   DS+LVLIAFDGTWKHAKEMVRSSEEFLS+FARR+CLDFD+ VDGGSIFD
Sbjct: 121 SELDLTSSSSPDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVDGGSIFD 180

Query: 181 SELILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRP 240
           SELILRKEPHGGCVSTMEAVARCLRVME++GAEIE RLIGVLREMVRLQA FLKPMNPRP
Sbjct: 181 SELILRKEPHGGCVSTMEAVARCLRVMEADGAEIEVRLIGVLREMVRLQAGFLKPMNPRP 240

Query: 241 KLLKMKKIMKKKKESED 255
           KLLK KK  K K E ED
Sbjct: 241 KLLKTKKKTKNKSERED 249

BLAST of Lag0011782 vs. NCBI nr
Match: XP_022967888.1 (DTW domain-containing protein 2 [Cucurbita maxima])

HSP 1 Score: 421.8 bits (1083), Expect = 4.2e-114
Identity = 217/257 (84.44%), Postives = 234/257 (91.05%), Query Frame = 0

Query: 1   MDPTNHHHRNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60
           M+PTNHHH  ++AA   ++SSSPPPRR+ICPNCTRPQ VCLCKFLPSPPIATKTQIIILQ
Sbjct: 1   MEPTNHHH--NSAAGYSSASSSPPPRRSICPNCTRPQLVCLCKFLPSPPIATKTQIIILQ 60

Query: 61  HPHEAQHKLSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNI 120
           HPHEAQHKLSTTPILTKCL NATTIV RRLKPGLS+LLDRSP AV+LFPPTPHSSPP NI
Sbjct: 61  HPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSSPPINI 120

Query: 121 SELDL---ASSDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFD 180
           SELDL   +SSDS+LVLIAFDGTWKHAKEMVRSSEEFLS+FARR+CLDFD+ VDGG+IFD
Sbjct: 121 SELDLTSSSSSDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVDGGTIFD 180

Query: 181 SELILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRP 240
           SELILRKEPHGGCVSTMEAVARCLRVME++GAEIE RLIGVLREMVRLQA FLKPMNPRP
Sbjct: 181 SELILRKEPHGGCVSTMEAVARCLRVMEADGAEIELRLIGVLREMVRLQAGFLKPMNPRP 240

Query: 241 KLLKMKKIMKKKKESED 255
           KL+K    MK K E ED
Sbjct: 241 KLVK----MKNKSERED 251

BLAST of Lag0011782 vs. NCBI nr
Match: KAG7033522.1 (DTW domain-containing protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 418.3 bits (1074), Expect = 4.7e-113
Identity = 215/257 (83.66%), Postives = 228/257 (88.72%), Query Frame = 0

Query: 1   MDPTNHHHRNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60
           M+ TNHHH         +++S PP RR+ICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ
Sbjct: 1   MESTNHHH--------NSAASPPPARRSICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60

Query: 61  HPHEAQHKLSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNI 120
           HPHEAQHKLSTTPILTKCL NATTIV RRLKPGLS+LLDRSP AV+LFPPTPHSSPP NI
Sbjct: 61  HPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSSPPINI 120

Query: 121 SELDLASS---DSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFD 180
           SELDL SS   DS+LVLIAFDGTWKHAKEMVRSSEEFLS+FARR+CLDFD+ VDGGSIFD
Sbjct: 121 SELDLTSSSSPDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVDGGSIFD 180

Query: 181 SELILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRP 240
           SELILRKEPHGGCVSTMEAVARCLRVME++GAEIE RLIGVLREMVRLQA FLKPMNPRP
Sbjct: 181 SELILRKEPHGGCVSTMEAVARCLRVMEADGAEIELRLIGVLREMVRLQAGFLKPMNPRP 240

Query: 241 KLLKMKKIMKKKKESED 255
           KLLK KK  K K E ED
Sbjct: 241 KLLKTKKKTKNKSERED 249

BLAST of Lag0011782 vs. NCBI nr
Match: XP_038883027.1 (tRNA-uridine aminocarboxypropyltransferase 2 [Benincasa hispida])

HSP 1 Score: 404.4 bits (1038), Expect = 7.0e-109
Identity = 207/254 (81.50%), Postives = 227/254 (89.37%), Query Frame = 0

Query: 1   MDPTNHHHRNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60
           M+ TN+H   + AA   +SSSSPPPRR+ICPNC+RPQPVCLCKFLPS PIATKT+II+LQ
Sbjct: 1   MEATNNH---NPAAGNSSSSSSPPPRRSICPNCSRPQPVCLCKFLPSSPIATKTKIILLQ 60

Query: 61  HPHEAQHKLSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNI 120
           HPHEAQHKLSTTPILTKCL NATTIV RRLKPGLSELLDRSPPAVFLFPPTPHS+PP NI
Sbjct: 61  HPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSELLDRSPPAVFLFPPTPHSTPPINI 120

Query: 121 SELDLASSDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFDSEL 180
           SEL++    ++LVLIAFDGTWKHAKEMVRSSEEFLS+FARRVCLDFD+ V+GGSIFDSEL
Sbjct: 121 SELNIT---ANLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDFDESVEGGSIFDSEL 180

Query: 181 ILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRPKLL 240
           ILRKEPHGGCVSTMEAVARCLRVME +GA IE++LI VLREMVRLQA FLKPMNPRPKLL
Sbjct: 181 ILRKEPHGGCVSTMEAVARCLRVMEPDGAAIEKKLIEVLREMVRLQAGFLKPMNPRPKLL 240

Query: 241 KMKKIMKKKKESED 255
           K+K   KK  E ED
Sbjct: 241 KIKNRKKKMSEGED 248

BLAST of Lag0011782 vs. ExPASy Swiss-Prot
Match: Q8NBA8 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Homo sapiens OX=9606 GN=DTWD2 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 5.0e-17
Identity = 65/210 (30.95%), Postives = 97/210 (46.19%), Query Frame = 0

Query: 23  PPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQHPHEAQHKLSTTPILTKCL--Q 82
           P  RR  C  C+RPQ VCLC FLP+ P+   T + I+QHP E    L T P+L  CL   
Sbjct: 62  PAERRPECTRCSRPQKVCLCPFLPAHPLHISTHLYIIQHPAEENKVLRTVPLLAACLPQD 121

Query: 83  NATTIVGRRLK----PGLSELLDRSPPAVFLFPPTPHSSPPTNISELDLASSDSDLVLIA 142
                +GRR      P LS +  R    + L+P     +   N+ E  L S      +I 
Sbjct: 122 KCKVKIGRRFSEERDPELSTVC-RKSGTLILYP----GAEAANLEEFILDSPVYPSTIII 181

Query: 143 FDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFDSELILRKEPHGGCVSTMEA 202
            DGTW  AK++   +  F  +  ++V L         +   S+ ++R +P   C+ST+E 
Sbjct: 182 IDGTWSQAKDIFYKNSLF--RHPKQVQLK--------TSISSQYVIRMQPTNRCLSTLEC 241

Query: 203 VARCLRVMESEGAEIERRLIGVLREMVRLQ 227
            A  L ++E     I+  L+  L+ +   Q
Sbjct: 242 AAVALSILEKNN-YIQETLLRPLQALCSFQ 255

BLAST of Lag0011782 vs. ExPASy Swiss-Prot
Match: Q4R7M4 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Macaca fascicularis OX=9541 GN=DTWD2 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.6e-15
Identity = 74/251 (29.48%), Postives = 109/251 (43.43%), Query Frame = 0

Query: 23  PPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQHPHEAQHKLSTTPILTKCL--Q 82
           P  RR  C  C+RPQ VCLC FLP+ P+   T + I+QHP E    L T P+L  CL   
Sbjct: 62  PAKRRPECSRCSRPQKVCLCPFLPAHPLHISTHLYIIQHPAEENKVLRTVPLLAACLPQD 121

Query: 83  NATTIVGRRLK----PGLSELLDRSPPAVFLFPPTPHSSPPTNISELDLASSDSDLVLIA 142
                +GRR      P LS +  R    + L+P     +   N+ E  L S      +I 
Sbjct: 122 KCKVKIGRRFSEERDPELSTVC-RKSGTLILYP----GAEAANLEEFILDSPVYPSTIII 181

Query: 143 FDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFDSELILRKEPHGGCVSTMEA 202
            DGTW  AK++   +  F  +  ++V L         +   S+ ++R +P   C+ST+E 
Sbjct: 182 IDGTWSQAKDIFYKNSLF--RHPKQVQLK--------TSISSQYVIRMQPTNRCLSTLEC 241

Query: 203 VARCLRVMESEGAEIERRL----------------IGVLREMVRLQAAFLKPM-NPRPKL 251
            A  L ++E      E  L                I + +E +     + KPM   + KL
Sbjct: 242 AAVALSILEKNNYIQETLLRPLQALCSFQLQHGAQIRLSKEHLLKNGLYTKPMPKNKRKL 297

BLAST of Lag0011782 vs. ExPASy Swiss-Prot
Match: Q9D0U1 (tRNA-uridine aminocarboxypropyltransferase 2 OS=Mus musculus OX=10090 GN=Dtwd2 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.0e-14
Identity = 79/270 (29.26%), Postives = 118/270 (43.70%), Query Frame = 0

Query: 13  AAATGTSSSSP---------PPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQHPH 72
           A A+G S+S+            RR  C  C+RPQ VCLC +LP  P+   T + I+QHP 
Sbjct: 43  AGASGDSTSADGLWGLPVEHAERRPECGRCSRPQKVCLCPYLPVRPLQISTHLYIIQHPA 102

Query: 73  EAQHKLSTTPILTKCL--QNATTIVGRRLKPGLSELLD---RSPPAVFLFPPTPHSSPPT 132
           E    L T P+L  CL     T  +GRR        L    R    + L+P     +  T
Sbjct: 103 EESRVLRTVPLLAACLPPDRCTVKIGRRFSEERDVELATVCRDSGTLILYP----GAEAT 162

Query: 133 NISELDLASSDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFDS 192
           N+ E  L S      +I  DGTW  AK++   +  F  +  ++V L         +   S
Sbjct: 163 NLEEFILDSPVYPSTIILIDGTWSQAKDIFYKNSLF--RLPKQVQLK--------TSVCS 222

Query: 193 ELILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREM----------VRLQAA 251
           + ++R +P   C+ST+E  A  L ++E     I+  L+  L+ +          +RL   
Sbjct: 223 QYVIRMQPTNRCLSTLECAAVALSILEKNNC-IQETLLRPLQALCSFQLQHGAQIRLSKE 282

BLAST of Lag0011782 vs. ExPASy TrEMBL
Match: A0A6J1EL33 (tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111435542 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 9.2e-115
Identity = 217/257 (84.44%), Postives = 230/257 (89.49%), Query Frame = 0

Query: 1   MDPTNHHHRNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60
           M+PTNHHH         +++SSPP RR+ICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ
Sbjct: 1   MEPTNHHH--------NSAASSPPARRSICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60

Query: 61  HPHEAQHKLSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNI 120
           HPHEAQHKLSTTPILTKCL NATTIV RRLKPGLS+LLDRSP AV+LFPPTPHSSPP NI
Sbjct: 61  HPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSSPPINI 120

Query: 121 SELDLASS---DSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFD 180
           SELDL SS   DS+LVLIAFDGTWKHAKEMVRSSEEFLS+FARR+CLDFD+ VDGGSIFD
Sbjct: 121 SELDLTSSSSPDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVDGGSIFD 180

Query: 181 SELILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRP 240
           SELILRKEPHGGCVSTMEAVARCLRVME++GAEIE RLIGVLREMVRLQA FLKPMNPRP
Sbjct: 181 SELILRKEPHGGCVSTMEAVARCLRVMEADGAEIEVRLIGVLREMVRLQAGFLKPMNPRP 240

Query: 241 KLLKMKKIMKKKKESED 255
           KLLK KK  K K E ED
Sbjct: 241 KLLKTKKKTKNKSERED 249

BLAST of Lag0011782 vs. ExPASy TrEMBL
Match: A0A6J1HWG2 (tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111467264 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 2.1e-114
Identity = 217/257 (84.44%), Postives = 234/257 (91.05%), Query Frame = 0

Query: 1   MDPTNHHHRNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60
           M+PTNHHH  ++AA   ++SSSPPPRR+ICPNCTRPQ VCLCKFLPSPPIATKTQIIILQ
Sbjct: 1   MEPTNHHH--NSAAGYSSASSSPPPRRSICPNCTRPQLVCLCKFLPSPPIATKTQIIILQ 60

Query: 61  HPHEAQHKLSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNI 120
           HPHEAQHKLSTTPILTKCL NATTIV RRLKPGLS+LLDRSP AV+LFPPTPHSSPP NI
Sbjct: 61  HPHEAQHKLSTTPILTKCLLNATTIVNRRLKPGLSDLLDRSPTAVYLFPPTPHSSPPINI 120

Query: 121 SELDL---ASSDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFD 180
           SELDL   +SSDS+LVLIAFDGTWKHAKEMVRSSEEFLS+FARR+CLDFD+ VDGG+IFD
Sbjct: 121 SELDLTSSSSSDSNLVLIAFDGTWKHAKEMVRSSEEFLSRFARRICLDFDESVDGGTIFD 180

Query: 181 SELILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRP 240
           SELILRKEPHGGCVSTMEAVARCLRVME++GAEIE RLIGVLREMVRLQA FLKPMNPRP
Sbjct: 181 SELILRKEPHGGCVSTMEAVARCLRVMEADGAEIELRLIGVLREMVRLQAGFLKPMNPRP 240

Query: 241 KLLKMKKIMKKKKESED 255
           KL+K    MK K E ED
Sbjct: 241 KLVK----MKNKSERED 251

BLAST of Lag0011782 vs. ExPASy TrEMBL
Match: A0A5A7TJW5 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold335G00690 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 6.6e-105
Identity = 202/254 (79.53%), Postives = 219/254 (86.22%), Query Frame = 0

Query: 1   MDPTNHHHRNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60
           M+ TN+H+      A G SSSSPPPRR ICPNC RPQPVCLCKFLPS PIATKT+IIILQ
Sbjct: 1   MESTNNHN-----FAAGYSSSSPPPRRPICPNCNRPQPVCLCKFLPSSPIATKTKIIILQ 60

Query: 61  HPHEAQHKLSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNI 120
           HPHE+QHKLSTTPILTKCL NATTIV RRLKPGLS+LLD+SPPAVFLFPPTPHSSPP NI
Sbjct: 61  HPHESQHKLSTTPILTKCLLNATTIVNRRLKPGLSKLLDQSPPAVFLFPPTPHSSPPINI 120

Query: 121 SELDLASSDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFDSEL 180
           S+L++     DLVLIAFDGTWKHAKEMVRSSEEFLS+FARRVCLD D+ V+GGSIFDSEL
Sbjct: 121 SDLNIT---EDLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDIDESVEGGSIFDSEL 180

Query: 181 ILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRPKLL 240
           ILRKEPHGGCVSTMEAVARCLRVME +GA +E +LIGVLREMVRLQA FLKPMNPRPKLL
Sbjct: 181 ILRKEPHGGCVSTMEAVARCLRVMELDGAAVEEKLIGVLREMVRLQAGFLKPMNPRPKLL 240

Query: 241 KMKKIMKKKKESED 255
           K  K      E E+
Sbjct: 241 KNFKQKTSVMEDEN 246

BLAST of Lag0011782 vs. ExPASy TrEMBL
Match: A0A5D3D883 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold307G00010 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 7.3e-104
Identity = 201/254 (79.13%), Postives = 218/254 (85.83%), Query Frame = 0

Query: 1   MDPTNHHHRNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQ 60
           M+ TN+H+      A G SSSS PPRR ICPNC RPQPVCLCKFLPS PIATKT+IIILQ
Sbjct: 1   MESTNNHN-----FAAGYSSSSRPPRRPICPNCNRPQPVCLCKFLPSSPIATKTKIIILQ 60

Query: 61  HPHEAQHKLSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNI 120
           HPHE+QHKLSTTPILTKCL NATTIV RRLKPGLS+LLD+SPPAVFLFPPTPHSSPP NI
Sbjct: 61  HPHESQHKLSTTPILTKCLLNATTIVNRRLKPGLSKLLDQSPPAVFLFPPTPHSSPPINI 120

Query: 121 SELDLASSDSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFDSEL 180
           S+L++     DLVLIAFDGTWKHAKEMVRSSEEFLS+FARRVCLD D+ V+GGSIFDSEL
Sbjct: 121 SDLNIT---EDLVLIAFDGTWKHAKEMVRSSEEFLSRFARRVCLDIDESVEGGSIFDSEL 180

Query: 181 ILRKEPHGGCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRPKLL 240
           ILRKEPHGGCVSTMEAVARCLRVME +GA +E +LIGVLREMVRLQA FLKPMNPRPKLL
Sbjct: 181 ILRKEPHGGCVSTMEAVARCLRVMELDGAAVEEKLIGVLREMVRLQAGFLKPMNPRPKLL 240

Query: 241 KMKKIMKKKKESED 255
           K  K      E E+
Sbjct: 241 KNFKQKTSVMEDEN 246

BLAST of Lag0011782 vs. ExPASy TrEMBL
Match: A0A0A0KW36 (tRNA-uridine aminocarboxypropyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G165850 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.8e-103
Identity = 197/246 (80.08%), Postives = 214/246 (86.99%), Query Frame = 0

Query: 9   RNSTAAATGTSSSSPPPRRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQHPHEAQHK 68
           RN+   A G SSSS PPRR+IC NC RPQPVCLCKFLPSPPIATKT+IIILQHPHE+QHK
Sbjct: 4   RNNHDLAAGYSSSSTPPRRSICSNCNRPQPVCLCKFLPSPPIATKTKIIILQHPHESQHK 63

Query: 69  LSTTPILTKCLQNATTIVGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNISELDLASS 128
           LSTTPILTKCL NATTIV RRLKPGLS+LLD+SP AVFLFPPTPHSSPP NIS+L++   
Sbjct: 64  LSTTPILTKCLLNATTIVNRRLKPGLSKLLDQSPHAVFLFPPTPHSSPPINISDLNIT-- 123

Query: 129 DSDLVLIAFDGTWKHAKEMVRSSEEFLSKFARRVCLDFDDGVDGGSIFDSELILRKEPHG 188
             DLVLIAFDGTWKHAKEMVRSSEE+LS+FARRVCLD D+ V+GGSIFDSELILRKEPHG
Sbjct: 124 -EDLVLIAFDGTWKHAKEMVRSSEEYLSRFARRVCLDIDESVEGGSIFDSELILRKEPHG 183

Query: 189 GCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRPKLLKMKKIMKK 248
           GCVSTMEAVARCLRVME +GA +E +LIGVLREMVRLQA FLKPMNPRPKLLK  K    
Sbjct: 184 GCVSTMEAVARCLRVMELDGAAVEEKLIGVLREMVRLQAGFLKPMNPRPKLLKSLKKQTS 243

Query: 249 KKESED 255
             E E+
Sbjct: 244 VTEDEN 246

BLAST of Lag0011782 vs. TAIR 10
Match: AT2G41750.1 (DTW domain-containing protein )

HSP 1 Score: 223.0 bits (567), Expect = 2.7e-58
Identity = 134/246 (54.47%), Postives = 165/246 (67.07%), Query Frame = 0

Query: 26  RRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQHPHEAQHKLSTTPILTKCLQNATTI 85
           RR IC NC RP  +CLC  LP+  I T T+IIIL HPHE++HKL+TTP+LTK L N T I
Sbjct: 10  RRQICDNCDRPNAICLCHVLPADLIPTNTEIIILHHPHESRHKLNTTPLLTKSLLNVTRI 69

Query: 86  VGRRL------KPGLSELLDRSPPAVFLFPPTPHSSPPTNISE---LDL-----ASSDSD 145
             RRL        G + L   +PP ++LFP +P SSP   ISE   L+L      S+   
Sbjct: 70  PARRLLRRHISTAGGTAL--PAPPTIYLFPSSP-SSPAVTISEFKSLNLLNHREISNPPP 129

Query: 146 LVLIAFDGTWKHAKEMVRSSEEFLSKF-ARRVCLD--FDDGVDGGSIFDSELILRKEPHG 205
           L LI FD TWKHAKEMV++SE  L +  A RVCLD   D  V GG+I+DSEL+LRKEP G
Sbjct: 130 LRLIVFDATWKHAKEMVKASEVVLREAGAVRVCLDTEIDASVSGGTIYDSELVLRKEPFG 189

Query: 206 GCVSTMEAVARCLRVMESEGAEIERRLIGVLREMVRLQAAFLKPMNPRPKLLKMKKIMKK 255
           GCV+T EAVARCL  +E +G EIER+LI VL+EMVR Q+ +LKPM PRPKLLK +   ++
Sbjct: 190 GCVTTAEAVARCLGAIEPDGEEIERKLISVLKEMVRFQSKYLKPMKPRPKLLKKRFQNQQ 249

BLAST of Lag0011782 vs. TAIR 10
Match: AT5G54880.1 (DTW domain-containing protein )

HSP 1 Score: 47.0 bits (110), Expect = 2.6e-05
Identity = 32/117 (27.35%), Postives = 52/117 (44.44%), Query Frame = 0

Query: 26  RRTICPNCTRPQPVCLCKFLPSPPIATKTQIIILQHPHEAQHKLSTTPILTKCLQNATTI 85
           +R  CP+C +P  +CLCK + SP    +  I ILQH  E +H L++T I    L+N    
Sbjct: 44  KRPTCPSCDKPSQLCLCKKMRSPCFDNQVSITILQHSLERKHALNSTRIARLGLKNV--- 103

Query: 86  VGRRLKPGLSELLDRSPPAVFLFPPTPHSSPPTNISELDLASSDSDLVLIAFDGTWK 143
                  G++ + D    A FL           + + LD      ++  +  D ++K
Sbjct: 104 -------GVTTVFDVHDEAEFLIRVIGSGCSKIDTNHLDSEYRVENVASLELDDSFK 150

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023544253.11.6e-11685.60DTW domain-containing protein 2 [Cucurbita pepo subsp. pepo][more]
XP_022928716.11.9e-11484.44DTW domain-containing protein 2 [Cucurbita moschata][more]
XP_022967888.14.2e-11484.44DTW domain-containing protein 2 [Cucurbita maxima][more]
KAG7033522.14.7e-11383.66DTW domain-containing protein 2, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_038883027.17.0e-10981.50tRNA-uridine aminocarboxypropyltransferase 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8NBA85.0e-1730.95tRNA-uridine aminocarboxypropyltransferase 2 OS=Homo sapiens OX=9606 GN=DTWD2 PE... [more]
Q4R7M41.6e-1529.48tRNA-uridine aminocarboxypropyltransferase 2 OS=Macaca fascicularis OX=9541 GN=D... [more]
Q9D0U11.0e-1429.26tRNA-uridine aminocarboxypropyltransferase 2 OS=Mus musculus OX=10090 GN=Dtwd2 P... [more]
Match NameE-valueIdentityDescription
A0A6J1EL339.2e-11584.44tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1HWG22.1e-11484.44tRNA-uridine aminocarboxypropyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5A7TJW56.6e-10579.53tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=119469... [more]
A0A5D3D8837.3e-10479.13tRNA-uridine aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=119469... [more]
A0A0A0KW364.8e-10380.08tRNA-uridine aminocarboxypropyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G1... [more]
Match NameE-valueIdentityDescription
AT2G41750.12.7e-5854.47DTW domain-containing protein [more]
AT5G54880.12.6e-0527.35DTW domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005636DTWSMARTSM01144DTW_2acoord: 26..231
e-value: 3.7E-46
score: 169.3
IPR005636DTWPFAMPF03942DTWcoord: 28..226
e-value: 3.0E-42
score: 144.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR21392:SF0DTW DOMAIN-CONTAINING PROTEIN 2coord: 5..242
IPR039262DTW domain-containing protein DTWD2/YfiPPANTHERPTHR21392UNCHARACTERIZEDcoord: 5..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0011782.1Lag0011782.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008033 tRNA processing
molecular_function GO:0016432 tRNA-uridine aminocarboxypropyltransferase activity