Lag0011661 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0011661
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Endoglucanase
Location	chr1: 30130879 .. 30131439 (-)
RNA-Seq Expression	Lag0011661
Synteny	Lag0011661

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGCGACGGCGATGGACGACGGCTTCTCCATCCTCCCCCACCACCGAATCCTCCCTTTCTTTCTTTCTTTCCCTTTCTTTCTTTCCCCTTTCCCCTCCCTTCTCCGAAATGGAAAATCTCCTCCTTGCCATTACTCTGCTCCTCGCAATCCTCGCCCCCTGTGCCAAGCTCTTTCCAAGAGCATTCTCTTCTTCGAAGCTCAGAGATCCGGCTACCTCCCCAACAACCAGTGCGTCACTTGGCGCGCCAACTCCGGCCTCCAAAACGGCAAGCCCAGCGGGGTAACTCCTTCCCCTTCTCTCAATTTCCTCGATTGGGAAACTGATTTGGGGATTTTATATATCATTCTGTCGTGTGCTGTATAGGTGGATCTGATCGGAGGTTTCTACGACGCCAACGAAAATGTGAAGTTCGGCCTACCGATGGCCTTCACCGTCACGATGATGAGTTGGAGCATTGTCGAATACGGGAAGCAAATGGCCGCCAATGGCGAACTCGACCATGCCCTAGACGCCGTCAAATGGGGCACCGGTTACCTCATCAAAGCCCACCAGTAA

mRNA sequence

ATGGACGCGACGGCGATGGACGACGGCTTCTCCATCCTCCCCCACCACCGAATCCTCCCTTTCTTTCTTTCTTTCCCTTTCTTTCTTTCCCCTTTCCCCTCCCTTCTCCGAAATGGAAAATCTCCTCCTTGCCATTACTCTGCTCCTCGCAATCCTCGCCCCCTGTGCCAAGCTCTTTCCAAGAGCATTCTCTTCTTCGAAGCTCAGAGATCCGGCTACCTCCCCAACAACCAGTGCGTCACTTGGCGCGCCAACTCCGGCCTCCAAAACGGCAAGCCCAGCGGGGTGGATCTGATCGGAGGTTTCTACGACGCCAACGAAAATGTGAAGTTCGGCCTACCGATGGCCTTCACCGTCACGATGATGAGTTGGAGCATTGTCGAATACGGGAAGCAAATGGCCGCCAATGGCGAACTCGACCATGCCCTAGACGCCGTCAAATGGGGCACCGGTTACCTCATCAAAGCCCACCAGTAA

Coding sequence (CDS)

ATGGACGCGACGGCGATGGACGACGGCTTCTCCATCCTCCCCCACCACCGAATCCTCCCTTTCTTTCTTTCTTTCCCTTTCTTTCTTTCCCCTTTCCCCTCCCTTCTCCGAAATGGAAAATCTCCTCCTTGCCATTACTCTGCTCCTCGCAATCCTCGCCCCCTGTGCCAAGCTCTTTCCAAGAGCATTCTCTTCTTCGAAGCTCAGAGATCCGGCTACCTCCCCAACAACCAGTGCGTCACTTGGCGCGCCAACTCCGGCCTCCAAAACGGCAAGCCCAGCGGGGTGGATCTGATCGGAGGTTTCTACGACGCCAACGAAAATGTGAAGTTCGGCCTACCGATGGCCTTCACCGTCACGATGATGAGTTGGAGCATTGTCGAATACGGGAAGCAAATGGCCGCCAATGGCGAACTCGACCATGCCCTAGACGCCGTCAAATGGGGCACCGGTTACCTCATCAAAGCCCACCAGTAA

Protein sequence

MDATAMDDGFSILPHHRILPFFLSFPFFLSPFPSLLRNGKSPPCHYSAPRNPRPLCQALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMAFTVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAHQ

Homology

[Close]

BLAST of Lag0011661 vs. NCBI nr
Match: KAG7031161.1 (Endoglucanase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 192.2 bits (487), Expect = 3.4e-45
Identity = 91/101 (90.10%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 57  QALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMA 116
           QALSKSILFFEAQRSGYLP+NQ VTWRANSGLQ+GK SGVDL GG+YDA +NVKFGLPMA
Sbjct: 27  QALSKSILFFEAQRSGYLPHNQRVTWRANSGLQDGKASGVDLTGGYYDAGDNVKFGLPMA 86

Query: 117 FTVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAH 158
           FTVTMMSWSIVEYGKQMAANGEL HALDAVKWGT YLIKAH
Sbjct: 87  FTVTMMSWSIVEYGKQMAANGELGHALDAVKWGTDYLIKAH 127

[Close]

BLAST of Lag0011661 vs. NCBI nr
Match: XP_023522541.1 (endoglucanase 6-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 192.2 bits (487), Expect = 3.4e-45
Identity = 91/101 (90.10%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 57  QALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMA 116
           QALSKSILFFEAQRSGYLP+NQ VTWRANSGLQ+GK SGVDL GG+YDA +NVKFGLPMA
Sbjct: 27  QALSKSILFFEAQRSGYLPHNQRVTWRANSGLQDGKASGVDLTGGYYDAGDNVKFGLPMA 86

Query: 117 FTVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAH 158
           FTVTMMSWSIVEYGKQMAANGEL HALDAVKWGT YLIKAH
Sbjct: 87  FTVTMMSWSIVEYGKQMAANGELGHALDAVKWGTDYLIKAH 127

[Close]

BLAST of Lag0011661 vs. NCBI nr
Match: KAG6600521.1 (Endoglucanase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 192.2 bits (487), Expect = 3.4e-45
Identity = 91/101 (90.10%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 57  QALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMA 116
           QALSKSILFFEAQRSGYLP+NQ VTWRANSGLQ+GK SGVDL GG+YDA +NVKFGLPMA
Sbjct: 27  QALSKSILFFEAQRSGYLPHNQRVTWRANSGLQDGKASGVDLTGGYYDAGDNVKFGLPMA 86

Query: 117 FTVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAH 158
           FTVTMMSWSIVEYGKQMAANGEL HALDAVKWGT YLIKAH
Sbjct: 87  FTVTMMSWSIVEYGKQMAANGELGHALDAVKWGTDYLIKAH 127

[Close]

BLAST of Lag0011661 vs. NCBI nr
Match: XP_022942087.1 (endoglucanase 6-like [Cucurbita moschata])

HSP 1 Score: 192.2 bits (487), Expect = 3.4e-45
Identity = 91/101 (90.10%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 57  QALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMA 116
           QALSKSILFFEAQRSGYLP+NQ VTWRANSGLQ+GK SGVDL GG+YDA +NVKFGLPMA
Sbjct: 27  QALSKSILFFEAQRSGYLPHNQRVTWRANSGLQDGKASGVDLTGGYYDAGDNVKFGLPMA 86

Query: 117 FTVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAH 158
           FTVTMMSWSIVEYGKQMAANGEL HALDAVKWGT YLIKAH
Sbjct: 87  FTVTMMSWSIVEYGKQMAANGELGHALDAVKWGTDYLIKAH 127

[Close]

BLAST of Lag0011661 vs. NCBI nr
Match: XP_023548395.1 (endoglucanase 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 192.2 bits (487), Expect = 3.4e-45
Identity = 91/101 (90.10%), Postives = 95/101 (94.06%), Query Frame = 0


Query: 57  QALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMA 116
           QALSKSILFFEAQRSGYLP+NQ VTWRANSGLQ+GK SGVDL GG+YDA +NVKFGLPMA
Sbjct: 27  QALSKSILFFEAQRSGYLPHNQRVTWRANSGLQDGKASGVDLTGGYYDAGDNVKFGLPMA 86

Query: 117 FTVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAH 158
           FTVTMMSWSIVEYGKQMAANGEL HALDAVKWGT YLIKAH
Sbjct: 87  FTVTMMSWSIVEYGKQMAANGELGHALDAVKWGTDYLIKAH 127

[Close]

BLAST of Lag0011661 vs. ExPASy Swiss-Prot
Match: Q8L7I0 (Endoglucanase 19 OS=Arabidopsis thaliana OX=3702 GN=At4g11050 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.4e-41
Identity = 77/101 (76.24%), Postives = 89/101 (88.12%), Query Frame = 0


Query: 57  QALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMA 116
           QALSKSILFFEAQRSG+LP NQ V+WR++SGL +GK SGVDL+GG+YDA +NVKFGLPMA
Sbjct: 29  QALSKSILFFEAQRSGHLPPNQRVSWRSHSGLYDGKSSGVDLVGGYYDAGDNVKFGLPMA 88

Query: 117 FTVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAH 158
           FTVT M WSI+EYG Q+ +NGEL HA+DAVKWGT Y IKAH
Sbjct: 89  FTVTTMCWSIIEYGGQLESNGELGHAIDAVKWGTDYFIKAH 129

[Close]

BLAST of Lag0011661 vs. ExPASy Swiss-Prot
Match: Q42059 (Endoglucanase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g64390 PE=2 SV=2)

HSP 1 Score: 168.7 bits (426), Expect = 5.3e-41
Identity = 74/101 (73.27%), Postives = 90/101 (89.11%), Query Frame = 0


Query: 57  QALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMA 116
           QALSKS+LFFEAQRSG LP NQ VTWR++SGL +GK SGV+L+GG+YDA +NVKFGLPMA
Sbjct: 28  QALSKSLLFFEAQRSGVLPRNQRVTWRSHSGLTDGKSSGVNLVGGYYDAGDNVKFGLPMA 87

Query: 117 FTVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAH 158
           FTVTMM+WS++EYG Q+ ANGEL +++DA+KWGT Y IKAH
Sbjct: 88  FTVTMMAWSVIEYGNQLQANGELGNSIDAIKWGTDYFIKAH 128

[Close]

BLAST of Lag0011661 vs. ExPASy Swiss-Prot
Match: Q5NAT8 (Endoglucanase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU7 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 6.9e-41
Identity = 74/100 (74.00%), Postives = 87/100 (87.00%), Query Frame = 0


Query: 58  ALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMAF 117
           ALSKSIL+FEAQRSG LP NQ + WRANSGL +GK +GVDL+GG+YDA +NVKFG PMAF
Sbjct: 45  ALSKSILYFEAQRSGVLPGNQRIAWRANSGLADGKANGVDLVGGYYDAGDNVKFGFPMAF 104

Query: 118 TVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAH 158
           TVTMM+WS++EYGKQMAA GEL HA+DAV+WG  Y +KAH
Sbjct: 105 TVTMMAWSVLEYGKQMAAAGELGHAMDAVRWGADYFVKAH 144

[Close]

BLAST of Lag0011661 vs. ExPASy Swiss-Prot
Match: Q6L4I2 (Endoglucanase 15 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0212300 PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 3.4e-40
Identity = 77/100 (77.00%), Postives = 89/100 (89.00%), Query Frame = 0


Query: 58  ALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMAF 117
           AL KSIL+F+AQRSG LP NQ V+WRA+SGL +GK +GVDL+GG+YDA +NVKFGLPMAF
Sbjct: 38  ALRKSILYFQAQRSGVLPPNQRVSWRASSGLFDGKANGVDLVGGYYDAGDNVKFGLPMAF 97

Query: 118 TVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAH 158
           TVTMMSWSI+EYGKQMAA GEL +A+DAVKWGT Y IKAH
Sbjct: 98  TVTMMSWSILEYGKQMAAAGELRNAMDAVKWGTDYFIKAH 137

[Close]

BLAST of Lag0011661 vs. ExPASy Swiss-Prot
Match: Q5NAT0 (Endoglucanase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU5 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 5.8e-40
Identity = 72/100 (72.00%), Postives = 88/100 (88.00%), Query Frame = 0


Query: 58  ALSKSILFFEAQRSGYLPNNQCVTWRANSGLQNGKPSGVDLIGGFYDANENVKFGLPMAF 117
           ALSKSIL+FEAQRSG LP +Q + WRANSGL +GK +GVDL+GG+YDA +NVKFGLPMAF
Sbjct: 44  ALSKSILYFEAQRSGVLPGSQRIAWRANSGLADGKANGVDLVGGYYDAGDNVKFGLPMAF 103

Query: 118 TVTMMSWSIVEYGKQMAANGELDHALDAVKWGTGYLIKAH 158
           TVTMM+WS++EYG++MAA GEL HA++A+KWGT Y  KAH
Sbjct: 104 TVTMMAWSVIEYGEEMAAAGELGHAVEAIKWGTDYFAKAH 143

The following BLAST results are available for this feature:

BLAST of Lag0011661 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7031161.1	3.4e-45	90.10	Endoglucanase 6 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023522541.1	3.4e-45	90.10	endoglucanase 6-like, partial [Cucurbita pepo subsp. pepo]	[more]
KAG6600521.1	3.4e-45	90.10	Endoglucanase 6, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022942087.1	3.4e-45	90.10	endoglucanase 6-like [Cucurbita moschata]	[more]
XP_023548395.1	3.4e-45	90.10	endoglucanase 6-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0011661 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L7I0	2.4e-41	76.24	Endoglucanase 19 OS=Arabidopsis thaliana OX=3702 GN=At4g11050 PE=2 SV=1	[more]
Q42059	5.3e-41	73.27	Endoglucanase 6 OS=Arabidopsis thaliana OX=3702 GN=At1g64390 PE=2 SV=2	[more]
Q5NAT8	6.9e-41	74.00	Endoglucanase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU7 PE=2 SV=1	[more]
Q6L4I2	3.4e-40	77.00	Endoglucanase 15 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0212300 PE=2 S...	[more]
Q5NAT0	5.8e-40	72.00	Endoglucanase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLU5 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012341	Six-hairpin glycosidase-like superfamily	GENE3D	1.50.10.10		coord: 54..158 e-value: 3.0E-42 score: 147.3
IPR001701	Glycoside hydrolase family 9	PFAM	PF00759	Glyco_hydro_9	coord: 57..157 e-value: 1.6E-34 score: 120.1
None	No IPR available	PANTHER	PTHR22298	ENDO-1,4-BETA-GLUCANASE	coord: 56..157
None	No IPR available	PANTHER	PTHR22298:SF133	ENDOGLUCANASE	coord: 56..157
IPR008928	Six-hairpin glycosidase superfamily	SUPERFAMILY	48208	Six-hairpin glycosidases	coord: 56..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0011661.1	Lag0011661.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030245	cellulose catabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0008810	cellulase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds