Lag0006341 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006341
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description(R)-mandelonitrile lyase 1-like
Locationchr6: 41419814 .. 41420221 (+)
RNA-Seq ExpressionLag0006341
SyntenyLag0006341
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTCACCTCTTCAAAAGTCATCGGAATTTCAGAAAACAACACCTACTACTTCCAATCCATCGCCAGTACTTCACCATTTTCAATTCCCAAATCTTTTAGCCTCCTCCCTCCCCAATCCACCTCCGCCAATCTCAGCTTAGCCCCCATCGCCGGAAAATACTCCGCCGTCCGCTCCGTCGGGTCGCTTCGGCTGAATTCTTCCACCGATGTGAAGAAGAGTCCCATTGTCCGATTCAATTACTATTCTCATCCTGATGATGTTGCGTTGTGTGTAAAAGGGCTGAGAAAAGTCGGAGATTTGCTTAAAACTCAAATGATTGAAAATGTTAAGACGAGGGATTTGGAGGGTAATAAAGACAATTCAGTTTCTGGGGATTCCTTTGCCGGAGAACTTGTCGGATGA

mRNA sequence

ATGGCTTTCACCTCTTCAAAAGTCATCGGAATTTCAGAAAACAACACCTACTACTTCCAATCCATCGCCAGTACTTCACCATTTTCAATTCCCAAATCTTTTAGCCTCCTCCCTCCCCAATCCACCTCCGCCAATCTCAGCTTAGCCCCCATCGCCGGAAAATACTCCGCCGTCCGCTCCGTCGGGTCGCTTCGGCTGAATTCTTCCACCGATGTGAAGAAGAGTCCCATTGTCCGATTCAATTACTATTCTCATCCTGATGATGTTGCGTTGTGTGTAAAAGGGCTGAGAAAAGTCGGAGATTTGCTTAAAACTCAAATGATTGAAAATGTTAAGACGAGGGATTTGGAGGGTAATAAAGACAATTCAGTTTCTGGGGATTCCTTTGCCGGAGAACTTGTCGGATGA

Coding sequence (CDS)

ATGGCTTTCACCTCTTCAAAAGTCATCGGAATTTCAGAAAACAACACCTACTACTTCCAATCCATCGCCAGTACTTCACCATTTTCAATTCCCAAATCTTTTAGCCTCCTCCCTCCCCAATCCACCTCCGCCAATCTCAGCTTAGCCCCCATCGCCGGAAAATACTCCGCCGTCCGCTCCGTCGGGTCGCTTCGGCTGAATTCTTCCACCGATGTGAAGAAGAGTCCCATTGTCCGATTCAATTACTATTCTCATCCTGATGATGTTGCGTTGTGTGTAAAAGGGCTGAGAAAAGTCGGAGATTTGCTTAAAACTCAAATGATTGAAAATGTTAAGACGAGGGATTTGGAGGGTAATAAAGACAATTCAGTTTCTGGGGATTCCTTTGCCGGAGAACTTGTCGGATGA

Protein sequence

MAFTSSKVIGISENNTYYFQSIASTSPFSIPKSFSLLPPQSTSANLSLAPIAGKYSAVRSVGSLRLNSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEGNKDNSVSGDSFAGELVG
Homology
BLAST of Lag0006341 vs. NCBI nr
Match: XP_023546914.1 ((R)-mandelonitrile lyase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 187.2 bits (474), Expect = 9.4e-44
Identity = 94/118 (79.66%), Postives = 105/118 (88.98%), Query Frame = 0

Query: 3   FTSSKVIGISENNTYYFQSIASTSPFSIPKSFSLLPPQSTSANLSLAPIAGKYSAVRSVG 62
           FTSSKVIGIS NNTYYFQSIASTSPFSIP SFSLLPP ST+ NLS+A I GK+S + SVG
Sbjct: 339 FTSSKVIGISTNNTYYFQSIASTSPFSIPPSFSLLPPNSTTVNLSIATIGGKFSKLTSVG 398

Query: 63  SLRLNSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEGNK 121
           SLRLNSSTDVK SPIVRFNYYS+P D+ LCV G+RKVGD LKTQ+I+N+KTRDLEGN+
Sbjct: 399 SLRLNSSTDVKTSPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNR 456

BLAST of Lag0006341 vs. NCBI nr
Match: XP_022929724.1 ((R)-mandelonitrile lyase 1-like [Cucurbita moschata])

HSP 1 Score: 186.0 bits (471), Expect = 2.1e-43
Identity = 93/118 (78.81%), Postives = 105/118 (88.98%), Query Frame = 0

Query: 3   FTSSKVIGISENNTYYFQSIASTSPFSIPKSFSLLPPQSTSANLSLAPIAGKYSAVRSVG 62
           FTSSKVIGIS NNTYYFQSIASTSPFSIP SFSLLPP STS NL++A I GK+S + SVG
Sbjct: 339 FTSSKVIGISNNNTYYFQSIASTSPFSIPPSFSLLPPNSTSVNLTVATIGGKFSKLASVG 398

Query: 63  SLRLNSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEGNK 121
           SLRLNSSTDVK +PIVRFNYYS+P D+ LCV G+RKVGD LKTQ+I+N+KTRDLEGN+
Sbjct: 399 SLRLNSSTDVKTNPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNR 456

BLAST of Lag0006341 vs. NCBI nr
Match: KAG7029801.1 ((R)-mandelonitrile lyase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 186.0 bits (471), Expect = 2.1e-43
Identity = 93/118 (78.81%), Postives = 105/118 (88.98%), Query Frame = 0

Query: 3    FTSSKVIGISENNTYYFQSIASTSPFSIPKSFSLLPPQSTSANLSLAPIAGKYSAVRSVG 62
            FTSSKVIGIS NNTYYFQSIA+TSPFSIP SFSLLPP STS NL++A I GK+S + SVG
Sbjct: 1268 FTSSKVIGISNNNTYYFQSIATTSPFSIPPSFSLLPPNSTSVNLTVATIGGKFSKLTSVG 1327

Query: 63   SLRLNSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEGNK 121
            SLRLNSSTDVK SPIVRFNYYS+P D+ LCV G+RKVGD LKTQ+I+N+KTRDLEGN+
Sbjct: 1328 SLRLNSSTDVKTSPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNR 1385

BLAST of Lag0006341 vs. NCBI nr
Match: XP_022997400.1 ((R)-mandelonitrile lyase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 185.3 bits (469), Expect = 3.6e-43
Identity = 92/118 (77.97%), Postives = 105/118 (88.98%), Query Frame = 0

Query: 3   FTSSKVIGISENNTYYFQSIASTSPFSIPKSFSLLPPQSTSANLSLAPIAGKYSAVRSVG 62
           FTSSKVIGIS NNTYYFQSIA+TSPFSIP SFSLLPP STS NL++A I GK+S + SVG
Sbjct: 339 FTSSKVIGISNNNTYYFQSIATTSPFSIPPSFSLLPPNSTSVNLTIATIGGKFSKLTSVG 398

Query: 63  SLRLNSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEGNK 121
           SLRLNSSTDVK +PIVRFNYYS+P D+ LCV G+RKVGD LKTQ+I+N+KTRDLEGN+
Sbjct: 399 SLRLNSSTDVKTTPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNR 456

BLAST of Lag0006341 vs. NCBI nr
Match: XP_022997401.1 ((R)-mandelonitrile lyase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 185.3 bits (469), Expect = 3.6e-43
Identity = 92/118 (77.97%), Postives = 105/118 (88.98%), Query Frame = 0

Query: 3   FTSSKVIGISENNTYYFQSIASTSPFSIPKSFSLLPPQSTSANLSLAPIAGKYSAVRSVG 62
           FTSSKVIGIS NNTYYFQSIA+TSPFSIP SFSLLPP STS NL++A I GK+S + SVG
Sbjct: 335 FTSSKVIGISNNNTYYFQSIATTSPFSIPPSFSLLPPNSTSVNLTIATIGGKFSKLTSVG 394

Query: 63  SLRLNSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEGNK 121
           SLRLNSSTDVK +PIVRFNYYS+P D+ LCV G+RKVGD LKTQ+I+N+KTRDLEGN+
Sbjct: 395 SLRLNSSTDVKTTPIVRFNYYSNPADIQLCVGGVRKVGDFLKTQIIQNIKTRDLEGNR 452

BLAST of Lag0006341 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.1e-14
Identity = 49/112 (43.75%), Postives = 70/112 (62.50%), Query Frame = 0

Query: 8   VIGISENNTYYFQSIASTSPFSIPKSFSLLPPQS-TSANLSLAPIAGKYSAVRSVGSLRL 67
           V+GI  +   Y+Q   S+ PFS P  FSL P  S    N + A I  +     S GS+ L
Sbjct: 346 VLGIRSD---YYQVSLSSLPFSTP-PFSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTL 405

Query: 68  NSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEG 119
           NSS+DV+ +P ++FNYYS+  D+A CV G++K+GDLL+T+ +E  K RD+ G
Sbjct: 406 NSSSDVRIAPNIKFNYYSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVLG 453

BLAST of Lag0006341 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 8.0e-14
Identity = 48/120 (40.00%), Postives = 72/120 (60.00%), Query Frame = 0

Query: 8   VIGISENNTYYFQSIASTSPFSIPKSFSLLPPQS-TSANLSLAPIAGKYSAVRSVGSLRL 67
           V+GIS +   ++Q   S+ PF+ P  FS  P  S    N + A  A K +   S GSL L
Sbjct: 345 VLGISND---FYQCSFSSLPFTTP-PFSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTL 404

Query: 68  NSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEGNKDNSVSG 127
            SS++V+ SP V+FNYYS+P D++ CV G++K+G+LL T  ++  K  DL G +  ++ G
Sbjct: 405 KSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILG 460

BLAST of Lag0006341 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 3.4e-12
Identity = 46/120 (38.33%), Postives = 70/120 (58.33%), Query Frame = 0

Query: 8   VIGISENNTYYFQSIASTSPFSIPKSFSLLPPQS-TSANLSLAPIAGKYSAVRSVGSLRL 67
           V+GIS +   ++Q   S+ PF+ P  F   P  S    N + A  A K +   S GSL L
Sbjct: 345 VLGISND---FYQCSFSSLPFTTP-PFGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTL 404

Query: 68  NSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEGNKDNSVSG 127
            SS++V+ SP V+FNYYS+  D++ CV G++K+G+LL T  ++  K  DL G +  ++ G
Sbjct: 405 KSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILG 460

BLAST of Lag0006341 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 7.5e-12
Identity = 44/112 (39.29%), Postives = 65/112 (58.04%), Query Frame = 0

Query: 8   VIGISENNTYYFQSIASTSPFSIPKSFSLLPPQS-TSANLSLAPIAGKYSAVRSVGSLRL 67
           V+GI+ +   ++Q   S+ PF  P  FS  P  S    N + A I  K     S G++ L
Sbjct: 346 VLGITSD---FYQCSISSLPFDTP-PFSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTL 405

Query: 68  NSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEG 119
           NSS+DV+  P V+FNYYS+  D++ CV G++K+G++L T  +E  K  DL G
Sbjct: 406 NSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPG 453

BLAST of Lag0006341 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 4.9e-11
Identity = 36/114 (31.58%), Postives = 70/114 (61.40%), Query Frame = 0

Query: 7   KVIGISENNTYYFQSIASTSPFSIPKSFSLLPPQSTSANLSLAPIAGKYSAVRSVGSLRL 66
           +V+G++E+   + ++ ++  PF+ P     +   ++   + +  I  K     S+G LRL
Sbjct: 349 QVVGVTEDGA-FLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL 408

Query: 67  NSSTDVKKSPIVRFNYYSHPDDVALCVKGLRKVGDLLKTQMIENVKTRDLEGNK 121
            +STDV+ +P+VRFNY+S P D+  CV G RK+G++L+++ +++   R+  GN+
Sbjct: 409 -ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNR 460

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023546914.19.4e-4479.66(R)-mandelonitrile lyase 1-like [Cucurbita pepo subsp. pepo][more]
XP_022929724.12.1e-4378.81(R)-mandelonitrile lyase 1-like [Cucurbita moschata][more]
KAG7029801.12.1e-4378.81(R)-mandelonitrile lyase 1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022997400.13.6e-4377.97(R)-mandelonitrile lyase 1-like isoform X1 [Cucurbita maxima][more]
XP_022997401.13.6e-4377.97(R)-mandelonitrile lyase 1-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O242432.1e-1443.75(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
P527068.0e-1440.00(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K23.4e-1238.33(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527077.5e-1239.29(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
Q9SSM24.9e-1131.58(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.410.40coord: 1..131
e-value: 2.8E-20
score: 74.7
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 4..128
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 4..128
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 40..119
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 59..123
e-value: 2.2E-7
score: 31.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006341.1Lag0006341.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors