Lag0005436 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005436
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionkinetochore protein SPC24 homolog
Locationchr6: 17645898 .. 17647250 (-)
RNA-Seq ExpressionLag0005436
SyntenyLag0005436
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATTTGCCTGTCACATGTATGGTGCAACTGTTTTTCTTCACCACACTTGTCACCAATGAGATTAATGAGCTAGACCGACAGAGAATTTCTGTTCAAGAAAGGAAGCAGGCGATGAAGAAACTTGAGAAACAAGAACTTAGGGCGCAGTGAGTATAATCTCTTTTTGTAGATGCCTTGTCATCTGTAATTGTCACTGTTAATTAGTAGCAATTAACTAATTCCTGTGAAATTTGAATCTATTTCATTATTGTGCAGTATCAAGTATTATCTGACAAGCACAATTTTTATGTATTTTGTTGGAAGTACAGAAGGTCTACCTTTTTATTTGTTTAAATTAAATTTAGTCCATATGCAGCTTTTTTGCTCCAATCGATTGCTTATGGTAGTTAGGTTACACTCCTATGAAGATGGATGATTATTTTTTAAAGCAATGAGCGTTTATAGTCTAATTTTGATGCATGATATGGATAATGTCAACCTAATTGTTTAACCTTTTGATCTTAAAGGCATGGAATCCCACCCCAACCAACAAGAAAGTAATATCCTTCTGTTATAGCTGTTACCTAGTTCTTTTTAATTTGTTCTTCTGAAGTAATTTAGTTCTGTCTGTGTCTGCATTTGAATTTCTGTCCTGTGTTGAAAACGGGAATTTTGTCTACAAGGTATTTGAAATTTGCTAAAAGATCAGAGTAGAAATAAAGGTAAGGTAACAACGATTAGTAAACAACACTACCAAGTGACAAAAGTTGTCGAAGTCGTTATTTATAGTTCCTCTATAGCAACTTGCAATTACCAATTACTTGTGTATACGGAGGTGGACTAGACTAATATCAGTAACTGTAGTCCATTCCAGAACTGAATTTACAATAGTTGCAATTACCATATCCTTTTTTTTAACTATGAACTTATAAACTGAGTGGCTGTCGAAGATCCTTGACAACCCAAGTCTCAGTATCTTTAATTGACATTAACCTGATAGTTCTCTATCTTCCAACAACCCAAGTCTCAGTATCTTTAATTGACATTAACCTGATAGTTCTCTATCTTCCAATTTGATGCATATTTTCTTGTTGTTTGCAGGAGGAAGCTTTCGATGTATGCTTCGGTCACTGATATAATACCAAACATGGACGATCTGGCTAAAATTTCTGGCCGTATCCTTTGTCTGAATTTATACTCTTTTCTGTTATATTTTCTCTTTCCTTTGCTATTGCTTTACCTTAACTTTAATCAACTACAGATATTGTGGATAGAAATAAACGGGTGGTACAGAAGTTTGAATTGGACCCGACTAAGATGTCTGCATTTGATATATGCAACGACATTTGGAATACGATTAATTCCCCATAG

mRNA sequence

ATGTATTTGCCTGTCACATGTATGGTGCAACTGTTTTTCTTCACCACACTTGTCACCAATGAGATTAATGAGCTAGACCGACAGAGAATTTCTGTTCAAGAAAGGAAGCAGGCGATGAAGAAACTTGAGAAACAAGAACTTAGGGCGCAGAGGAAGCTTTCGATGTATGCTTCGGTCACTGATATAATACCAAACATGGACGATCTGGCTAAAATTTCTGGCCATATTGTGGATAGAAATAAACGGGTGGTACAGAAGTTTGAATTGGACCCGACTAAGATGTCTGCATTTGATATATGCAACGACATTTGGAATACGATTAATTCCCCATAG

Coding sequence (CDS)

ATGTATTTGCCTGTCACATGTATGGTGCAACTGTTTTTCTTCACCACACTTGTCACCAATGAGATTAATGAGCTAGACCGACAGAGAATTTCTGTTCAAGAAAGGAAGCAGGCGATGAAGAAACTTGAGAAACAAGAACTTAGGGCGCAGAGGAAGCTTTCGATGTATGCTTCGGTCACTGATATAATACCAAACATGGACGATCTGGCTAAAATTTCTGGCCATATTGTGGATAGAAATAAACGGGTGGTACAGAAGTTTGAATTGGACCCGACTAAGATGTCTGCATTTGATATATGCAACGACATTTGGAATACGATTAATTCCCCATAG

Protein sequence

MYLPVTCMVQLFFFTTLVTNEINELDRQRISVQERKQAMKKLEKQELRAQRKLSMYASVTDIIPNMDDLAKISGHIVDRNKRVVQKFELDPTKMSAFDICNDIWNTINSP
Homology
BLAST of Lag0005436 vs. NCBI nr
Match: XP_023518318.1 (uncharacterized protein LOC111781837 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 172.2 bits (435), Expect = 2.5e-39
Identity = 87/94 (92.55%), Postives = 91/94 (96.81%), Query Frame = 0

Query: 17  LVTNEINELDRQRISVQERKQAMKKLEKQELRAQRKLSMYASVTDIIPNMDDLAKISGHI 76
           LVTN+INELDRQRISVQERKQA KKLE+QELRAQRKLSMYASVTDIIPNMDD +KISGHI
Sbjct: 108 LVTNQINELDRQRISVQERKQATKKLEQQELRAQRKLSMYASVTDIIPNMDDHSKISGHI 167

Query: 77  VDRNKRVVQKFELDPTKMSAFDICNDIWNTINSP 111
           VDRNKRVVQKFELDPTKMS+FDICNDIWN INSP
Sbjct: 168 VDRNKRVVQKFELDPTKMSSFDICNDIWNMINSP 201

BLAST of Lag0005436 vs. NCBI nr
Match: XP_022966574.1 (uncharacterized protein LOC111466216 [Cucurbita maxima])

HSP 1 Score: 169.9 bits (429), Expect = 1.3e-38
Identity = 86/94 (91.49%), Postives = 90/94 (95.74%), Query Frame = 0

Query: 17  LVTNEINELDRQRISVQERKQAMKKLEKQELRAQRKLSMYASVTDIIPNMDDLAKISGHI 76
           LVTN+INELDRQRISVQERKQA KKLE+QELRAQRKLSMYASVTDIIPNMDD +KISGHI
Sbjct: 107 LVTNQINELDRQRISVQERKQATKKLEQQELRAQRKLSMYASVTDIIPNMDDHSKISGHI 166

Query: 77  VDRNKRVVQKFELDPTKMSAFDICNDIWNTINSP 111
           VDRNKRVVQKFELDPTK S+FDICNDIWN INSP
Sbjct: 167 VDRNKRVVQKFELDPTKTSSFDICNDIWNMINSP 200

BLAST of Lag0005436 vs. NCBI nr
Match: XP_022931907.1 (uncharacterized protein LOC111438184 [Cucurbita moschata] >XP_022931908.1 uncharacterized protein LOC111438184 [Cucurbita moschata])

HSP 1 Score: 169.5 bits (428), Expect = 1.6e-38
Identity = 85/94 (90.43%), Postives = 91/94 (96.81%), Query Frame = 0

Query: 17  LVTNEINELDRQRISVQERKQAMKKLEKQELRAQRKLSMYASVTDIIPNMDDLAKISGHI 76
           LV+N+INELDRQRISVQE+KQA KKLE+QELRAQRKLSMYASVTDIIPNMDD +KISGHI
Sbjct: 108 LVSNQINELDRQRISVQEKKQATKKLEQQELRAQRKLSMYASVTDIIPNMDDHSKISGHI 167

Query: 77  VDRNKRVVQKFELDPTKMSAFDICNDIWNTINSP 111
           VDRNKRVVQKFELDPTKMS+FDICNDIWN INSP
Sbjct: 168 VDRNKRVVQKFELDPTKMSSFDICNDIWNMINSP 201

BLAST of Lag0005436 vs. NCBI nr
Match: KAG6603567.1 (Kinetochore protein SPC24-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 167.5 bits (423), Expect = 6.3e-38
Identity = 83/103 (80.58%), Postives = 93/103 (90.29%), Query Frame = 0

Query: 8   MVQLFFFTTLVTNEINELDRQRISVQERKQAMKKLEKQELRAQRKLSMYASVTDIIPNMD 67
           M+ L F  T++T+EIN+LDRQRISVQERKQAMKKLE+QELR QRKLSMYASVTDIIPNMD
Sbjct: 1   MMHLLFVNTIITSEINDLDRQRISVQERKQAMKKLEQQELRTQRKLSMYASVTDIIPNMD 60

Query: 68  DLAKISGHIVDRNKRVVQKFELDPTKMSAFDICNDIWNTINSP 111
           D +KISGHIVDRNKRVVQ+FE DPT +S+FDICN IWN INSP
Sbjct: 61  DQSKISGHIVDRNKRVVQEFEFDPTNISSFDICNGIWNMINSP 103

BLAST of Lag0005436 vs. NCBI nr
Match: KAG7027420.1 (hypothetical protein SDJN02_11433, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 166.8 bits (421), Expect = 1.1e-37
Identity = 84/93 (90.32%), Postives = 88/93 (94.62%), Query Frame = 0

Query: 18  VTNEINELDRQRISVQERKQAMKKLEKQELRAQRKLSMYASVTDIIPNMDDLAKISGHIV 77
           +  EINELDRQRISVQERKQA KKLE+QELRAQRKLSMYASVTDIIPNMDD +KISGHIV
Sbjct: 92  IEEEINELDRQRISVQERKQATKKLEQQELRAQRKLSMYASVTDIIPNMDDHSKISGHIV 151

Query: 78  DRNKRVVQKFELDPTKMSAFDICNDIWNTINSP 111
           DRNKRVVQKFELDPTKMS+FDICNDIWN INSP
Sbjct: 152 DRNKRVVQKFELDPTKMSSFDICNDIWNMINSP 184

BLAST of Lag0005436 vs. ExPASy Swiss-Prot
Match: Q67XT3 (Kinetochore protein SPC24 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC24 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 2.5e-21
Identity = 46/92 (50.00%), Postives = 73/92 (79.35%), Query Frame = 0

Query: 17  LVTNEINELDRQRISVQERKQAMKKLEKQELRAQRKLSMYASVTDIIPNMDDLAKISGHI 76
           LV +E+ +L+ Q  S+ E KQ+ K+  + +LRA++KLSMYASVT++IP++DD +KISG++
Sbjct: 108 LVADELKDLNAQLSSIDEHKQSTKRKVRDDLRAEKKLSMYASVTNVIPDIDDPSKISGYM 167

Query: 77  VDRNKRVVQKFELDPTKMSAFDICNDIWNTIN 109
           VDR KR+++KF+ +  KM+A++ CN IW+ IN
Sbjct: 168 VDREKRLIEKFQFETNKMTAYETCNSIWSIIN 199

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023518318.12.5e-3992.55uncharacterized protein LOC111781837 [Cucurbita pepo subsp. pepo][more]
XP_022966574.11.3e-3891.49uncharacterized protein LOC111466216 [Cucurbita maxima][more]
XP_022931907.11.6e-3890.43uncharacterized protein LOC111438184 [Cucurbita moschata] >XP_022931908.1 unchar... [more]
KAG6603567.16.3e-3880.58Kinetochore protein SPC24-like protein, partial [Cucurbita argyrosperma subsp. s... [more]
KAG7027420.11.1e-3790.32hypothetical protein SDJN02_11433, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
Q67XT32.5e-2150.00Kinetochore protein SPC24 homolog OS=Arabidopsis thaliana OX=3702 GN=SPC24 PE=1 ... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 22..52
NoneNo IPR availableGENE3D3.30.160.570Ncd80 complex, Spc24 subunitcoord: 48..109
e-value: 3.3E-10
score: 41.6
IPR013252Kinetochore-Ndc80 subunit Spc24PFAMPF08286Spc24coord: 20..105
e-value: 6.2E-6
score: 26.4
IPR044951Kinetochore protein SPC24-likePANTHERPTHR35730KINETOCHORE PROTEIN SPC24 HOMOLOG-RELATEDcoord: 18..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005436.1Lag0005436.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051983 regulation of chromosome segregation