Lag0005405 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005405
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	NLR family CARD domain-containing protein 3
Location	chr6: 16893335 .. 16896192 (+)
RNA-Seq Expression	Lag0005405
Synteny	Lag0005405

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTACGAGGAACTGAAGAGCATACCTTGGGCATCCATGAGTATGGTATTTATGGGCATGAAAGTTTGTACCATATCACAGAGTATGGTATTTATACGTCTCTGTTTCTTATTGAAAAAAATAAGGCATGAAACTTTGTACGTTATGTCTTTGTAAGGGATCAGGGATGAGCAAGGATCTTGCCTAGGATCAAGCAAGCAACAGCGTTGCGTCTTGAAAGATGAGATCTTATGATCTGTTGAGCTAGTTGAATGATTGGGAAGCATAGACATCCTACTACTTCCCTAACAAGATCTTGAGTTGATGTACTGAACCCATATGCCTCATTTAGATTGAGTTTTTTTTTCCTTCTCATTTTTAATGAGAAATTGAGCTTTTGTTTTGTCTGGATTGAGTTGTGTTGCAACAAATTGATGGGTAGCATTGTTGATACTTAGATCGTCTCTGGGGTTGGTTCAATCATGAATTGAGCAGCTAGTTCGGAGACAATCCATGCAATAACAACTTTTATTCTTTGGGGGAACTATTTGTTAGCTGTGCTTCTTTAGAGATCCAATTAGGACTTCTTTTAAGACCATTTTTCAATTTTATCCTAACATCAGGAGTGGACAATGTTAGTCTTATTTATCATATAGTTTACACCTCACCTAAATAAAGCAAACGACTCAGGTTGGTCCTTGGCTCCAAGGAGGATTTGTGCCATATGATAAAACATAACTTGAGAATTGTTTAACCCAACAGCAATGTGCCATCAGTCAGGGGCTCAATTTACACTTGTAACGTATGATAAAACATAATTTGGAAACATGAAGTTCCTTTCTAACATTTGTGGATATGTATATTTTTAATTTGTTTAATTGACATGCACTCCCTTGTCACCTATTGAATTTCTTAAATTTGTTTAATTGACAGGAACTTCACATCAATGGAAATTCAATTGGTGATGAGGGAGTGCGTGCTTTAATTTCAGGTTTATCTTCACGTAAAGGTATGCCATATGATGCCATTCTACGTTCTTTCTGTTGTTGAAGTTTGAGCTTGATCAAATTCACATGCACGATATTTTATGCATTTGCCATTCGTTAACTTCAACAACATTCTTACTTCCTTGTAGGAAAACTTGCACTTCTTGACATTGGCAACAACTCAATCACTGCCAAGGGTGTCTTTCATGTTGCCGAATTCATTAAGAGAACTAAGAGCTTGGTTTTATTGAATTTGTTCATGAATGATATTGGTGATGAGGTAACGGGCAGAGCTTCTAAAATTACTCTTAGTATTTAACTTTTATAATATTTAAGTATTCTGTTAAGTTGTTGAATTTCAGTATTATGGGCTTCTTCCCTTGAGGAAGTTACATGAATAACACAAAATACCAAATCCAAGGTAGCATGATACTTGAACTTCTCTCTTAATCAAAGACAAAAAGAGAAAAAAAACCATGGAGATCCAAATGCATCTGAAAGCCACTGCTATCTGTGAGGGGCAGTCTATGGGATGAGCCATAGTGAATAAATCAGTTCTTTTTTCCCTTTTGTGGGTTGGTTTTTTGTATGCCTGCATATTCTTTCATTTTTCTCCATGAAAGTTTGGTTCCTTATCTGAAAAAAAACTTGTAAAGAGTGAAAATCATAAAACAAACCAATAGAAATGCAAAGTAGCCTATAAAGATTGAATAAAAGCACCCCAATTAATACAAATATCCCAAGCAGAATAAGACTCAAATAAATTAGAAAAAGGACACTAATGAGAAACCTTAAACTTAACAATATCAAATCATTCCATCAATGATTTACTTTTACCTTAAAAAATTATTTGGTTTCACTCAAACTGTAATAAGATAAAATGGATTTGACAGCGTTGACCCATAAAAGATGAGCTTGAGAAGATAAGGACGACCCATGAAGAAGCTGAAATAAATTATTTCTGGAATCCTTCGAGAATACCCAATCAAGATTAAAAATAGTAAACGAAGTGAACCAGCTTGATCACAAGTTTGTGAGGATGAAATCCAGGAATTTTTTATAGATGCATGTTTCCCGCTGGAGAGATTGTATAGCCTTGAGATTATCATCTAGAAAGGGTCACCACCACAAGTTGGTGGGAAAATCCTTGCCCACTGATTTCTTGCTACTTATATTTGATAATTGACTTCCAAAGACCTTTGGAGGTTTTATTATTATTATTTTTAATGAAATATCGAACTTTCAATGAGAAAAAGATGAAAGAATACAAGGATATACAAAAATAAAGCTCACAAAAAGGAGCCAAACTAAAAAAGGGCTCTAATCAAGTAAAATAAGACCCAGAGGATAATTACAAAATAACTTCGTTATCAAAGCCTGCAAAGGAATATGAAAGCTAACAAGGTACCAAACATTATCTGAAGGTCTCTCCAACCCTCTAATAATGTGTGAGACCTCCAATTATAAAAACGTACGAAAGAAGGGGCAAAATGGTCAAGGAGGACCAAACTAAGAAAGATAACAGAAATGGAGTGGGGACCAGCAAAGAATGATCGTGGGAACGTGTTATAGGCGTACGGGAGCAGAGCAAATGTCCTTATTAGGAGGATGGTTGTCTTTTTCGTTTTCTTTATGCATTTTGTGAGATTTAGAAAGTTTGTGTGCTTTCTGTGGAGAGGTACCAGGGCTCTCGAATCCCCTGTGTGTTAGTGTGTTTTCTTCTTTCGTTGTAATCGTTGAACCTTGGTTTTCGGGCTGTTTTCACTGTTCATCTCAATAGAACAGTGCTCCATTTGCCTATTGTGTGTGTTCTTTGGTTGTGTTTCGTTTTTCTTGCAGGAAAGCAGTTTGGATACCCAACATAATGCTATCATTTTGTTCTTCTCATAG

mRNA sequence

ATGACTTACGAGGAACTGAAGAGCATACCTTGGGCATCCATGAGTATGGAACTTCACATCAATGGAAATTCAATTGGTGATGAGGGAGTGCGTGCTTTAATTTCAGGTTTATCTTCACGTAAAGGAAAACTTGCACTTCTTGACATTGGCAACAACTCAATCACTGCCAAGGGTGTCTTTCATGTTGCCGAATTCATTAAGAGAACTAAGAGCTTGGTTTTATTGAATTTGTTCATGAATGATATTGGTGATGAGGAAAGCAGTTTGGATACCCAACATAATGCTATCATTTTGTTCTTCTCATAG

Coding sequence (CDS)

ATGACTTACGAGGAACTGAAGAGCATACCTTGGGCATCCATGAGTATGGAACTTCACATCAATGGAAATTCAATTGGTGATGAGGGAGTGCGTGCTTTAATTTCAGGTTTATCTTCACGTAAAGGAAAACTTGCACTTCTTGACATTGGCAACAACTCAATCACTGCCAAGGGTGTCTTTCATGTTGCCGAATTCATTAAGAGAACTAAGAGCTTGGTTTTATTGAATTTGTTCATGAATGATATTGGTGATGAGGAAAGCAGTTTGGATACCCAACATAATGCTATCATTTTGTTCTTCTCATAG

Protein sequence

MTYEELKSIPWASMSMELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLNLFMNDIGDEESSLDTQHNAIILFFS

Homology

[Close]

BLAST of Lag0005405 vs. NCBI nr
Match: XP_022999141.1 (NLR family CARD domain-containing protein 3 [Cucurbita maxima])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 66/69 (95.65%), Postives = 69/69 (100.00%), Query Frame = 0


Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           ELH+NGNS+GDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEF+KRTKSLVLLN
Sbjct: 348 ELHMNGNSVGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFMKRTKSLVLLN 407

Query: 77  LFMNDIGDE 86
           LFMNDIGDE
Sbjct: 408 LFMNDIGDE 416

[Close]

BLAST of Lag0005405 vs. NCBI nr
Match: KAG6599019.1 (NLR family CARD domain-containing protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 66/69 (95.65%), Postives = 69/69 (100.00%), Query Frame = 0


Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           ELH+NGNS+GDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEF+KRTKSLVLLN
Sbjct: 348 ELHMNGNSVGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFMKRTKSLVLLN 407

Query: 77  LFMNDIGDE 86
           LFMNDIGDE
Sbjct: 408 LFMNDIGDE 416

[Close]

BLAST of Lag0005405 vs. NCBI nr
Match: XP_023520466.1 (NLR family CARD domain-containing protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 66/69 (95.65%), Postives = 69/69 (100.00%), Query Frame = 0


Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           ELH+NGNS+GDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEF+KRTKSLVLLN
Sbjct: 348 ELHMNGNSVGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFMKRTKSLVLLN 407

Query: 77  LFMNDIGDE 86
           LFMNDIGDE
Sbjct: 408 LFMNDIGDE 416

[Close]

BLAST of Lag0005405 vs. NCBI nr
Match: KAG7029980.1 (NLR family CARD domain-containing protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 66/69 (95.65%), Postives = 69/69 (100.00%), Query Frame = 0


Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           ELH+NGNS+GDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEF+KRTKSLVLLN
Sbjct: 396 ELHMNGNSVGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFMKRTKSLVLLN 455

Query: 77  LFMNDIGDE 86
           LFMNDIGDE
Sbjct: 456 LFMNDIGDE 464

[Close]

BLAST of Lag0005405 vs. NCBI nr
Match: XP_022946525.1 (NLR family CARD domain-containing protein 3 [Cucurbita moschata])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 66/69 (95.65%), Postives = 69/69 (100.00%), Query Frame = 0


Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           ELH+NGNS+GDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEF+KRTKSLVLLN
Sbjct: 348 ELHMNGNSVGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFMKRTKSLVLLN 407

Query: 77  LFMNDIGDE 86
           LFMNDIGDE
Sbjct: 408 LFMNDIGDE 416

[Close]

BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match: A6QLE5 (NACHT, LRR and PYD domains-containing protein 3 OS=Bos taurus OX=9913 GN=NLRP3 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 6.7e-05
Identity = 25/67 (37.31%), Postives = 41/67 (61.19%), Query Frame = 0


Query: 18  LHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLNL 77
           L++ GN++GD GV+ L  GL  R  KL +L++ N S+T+   + ++  +   +SL  L L
Sbjct: 912 LYLQGNALGDMGVKLLCEGLLHRNCKLQVLELDNCSLTSHCCWDLSTLLTSNQSLRKLCL 971

Query: 78  FMNDIGD 85
             ND+GD
Sbjct: 972 GNNDLGD 978

[Close]

BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match: Q96P20 (NACHT, LRR and PYD domains-containing protein 3 OS=Homo sapiens OX=9606 GN=NLRP3 PE=1 SV=3)

HSP 1 Score: 46.6 bits (109), Expect = 1.9e-04
Identity = 22/67 (32.84%), Postives = 43/67 (64.18%), Query Frame = 0


Query: 18  LHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLNL 77
           L++ GN++GD+G++ L  GL     KL +L++ N ++T+   + ++  +  ++SL  L+L
Sbjct: 917 LYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSL 976

Query: 78  FMNDIGD 85
             ND+GD
Sbjct: 977 GNNDLGD 983

[Close]

BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match: B0FPE9 (NACHT, LRR and PYD domains-containing protein 3 OS=Macaca mulatta OX=9544 GN=NLRP3 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.9e-04
Identity = 22/67 (32.84%), Postives = 43/67 (64.18%), Query Frame = 0


Query: 18  LHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLNL 77
           L++ GN++GD+G++ L  GL     KL +L++ N ++T+   + ++  +  ++SL  L+L
Sbjct: 916 LYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSL 975

Query: 78  FMNDIGD 85
             ND+GD
Sbjct: 976 GNNDLGD 982

[Close]

BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match: A6H639 (Dynein regulatory complex subunit 5 OS=Mus musculus OX=10090 GN=Tcte1 PE=1 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 7.4e-04
Identity = 27/69 (39.13%), Postives = 38/69 (55.07%), Query Frame = 0


Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           EL ++ N IGD G RA    LS    +L +L++ NN + A G   +A  +    +LV LN
Sbjct: 309 ELDLSHNLIGDRGARAAAKLLS--HSRLRVLNLANNQLQAPGAQSLAHALAHNTNLVFLN 368

Query: 77  LFMNDIGDE 86
           L +N I DE
Sbjct: 369 LRLNCIEDE 375

[Close]

BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match: O75864 (Protein phosphatase 1 regulatory subunit 37 OS=Homo sapiens OX=9606 GN=PPP1R37 PE=1 SV=4)

HSP 1 Score: 44.3 bits (103), Expect = 9.6e-04
Identity = 27/75 (36.00%), Postives = 42/75 (56.00%), Query Frame = 0


Query: 8   SIPWASMSMELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIK 67
           ++P       L++  N IG+EGVR L +GL S +  L  L + +  +T +G   VAEFI 
Sbjct: 328 TLPHTQSLETLNLGHNPIGNEGVRHLKNGLISNRSVLR-LGLASTKLTCEGAVAVAEFIA 387

Query: 68  RTKSLVLLNLFMNDI 83
            +  L+ L+L  N+I
Sbjct: 388 ESPRLLRLDLRENEI 401

The following BLAST results are available for this feature:

BLAST of Lag0005405 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022999141.1	1.2e-27	95.65	NLR family CARD domain-containing protein 3 [Cucurbita maxima]	[more]
KAG6599019.1	1.2e-27	95.65	NLR family CARD domain-containing protein 3, partial [Cucurbita argyrosperma sub...	[more]
XP_023520466.1	1.2e-27	95.65	NLR family CARD domain-containing protein 3 [Cucurbita pepo subsp. pepo]	[more]
KAG7029980.1	1.2e-27	95.65	NLR family CARD domain-containing protein 3, partial [Cucurbita argyrosperma sub...	[more]
XP_022946525.1	1.2e-27	95.65	NLR family CARD domain-containing protein 3 [Cucurbita moschata]	[more]

BLAST of Lag0005405 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A6QLE5	6.7e-05	37.31	NACHT, LRR and PYD domains-containing protein 3 OS=Bos taurus OX=9913 GN=NLRP3 P...	[more]
Q96P20	1.9e-04	32.84	NACHT, LRR and PYD domains-containing protein 3 OS=Homo sapiens OX=9606 GN=NLRP3...	[more]
B0FPE9	1.9e-04	32.84	NACHT, LRR and PYD domains-containing protein 3 OS=Macaca mulatta OX=9544 GN=NLR...	[more]
A6H639	7.4e-04	39.13	Dynein regulatory complex subunit 5 OS=Mus musculus OX=10090 GN=Tcte1 PE=1 SV=1	[more]
O75864	9.6e-04	36.00	Protein phosphatase 1 regulatory subunit 37 OS=Homo sapiens OX=9606 GN=PPP1R37 P...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00368	LRR_RI_2	coord: 41..68 e-value: 4.3 score: 12.7 coord: 13..39 e-value: 1.3 score: 16.3
None	No IPR available	PANTHER	PTHR24106:SF267	LEUCINE RICH REPEAT FAMILY PROTEIN	coord: 16..85
None	No IPR available	PANTHER	PTHR24106	NACHT, LRR AND CARD DOMAINS-CONTAINING	coord: 16..85
None	No IPR available	SUPERFAMILY	52047	RNI-like	coord: 17..85
IPR001611	Leucine-rich repeat	PFAM	PF13516	LRR_6	coord: 72..85 e-value: 1.5 score: 9.1 coord: 17..33 e-value: 0.0037 score: 17.2
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 6..96 e-value: 5.0E-15 score: 57.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005405.1	Lag0005405.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005515	protein binding
molecular_function	GO:0016740	transferase activity