Lag0005405 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005405
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionNLR family CARD domain-containing protein 3
Locationchr6: 16893335 .. 16896192 (+)
RNA-Seq ExpressionLag0005405
SyntenyLag0005405
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTACGAGGAACTGAAGAGCATACCTTGGGCATCCATGAGTATGGTATTTATGGGCATGAAAGTTTGTACCATATCACAGAGTATGGTATTTATACGTCTCTGTTTCTTATTGAAAAAAATAAGGCATGAAACTTTGTACGTTATGTCTTTGTAAGGGATCAGGGATGAGCAAGGATCTTGCCTAGGATCAAGCAAGCAACAGCGTTGCGTCTTGAAAGATGAGATCTTATGATCTGTTGAGCTAGTTGAATGATTGGGAAGCATAGACATCCTACTACTTCCCTAACAAGATCTTGAGTTGATGTACTGAACCCATATGCCTCATTTAGATTGAGTTTTTTTTTCCTTCTCATTTTTAATGAGAAATTGAGCTTTTGTTTTGTCTGGATTGAGTTGTGTTGCAACAAATTGATGGGTAGCATTGTTGATACTTAGATCGTCTCTGGGGTTGGTTCAATCATGAATTGAGCAGCTAGTTCGGAGACAATCCATGCAATAACAACTTTTATTCTTTGGGGGAACTATTTGTTAGCTGTGCTTCTTTAGAGATCCAATTAGGACTTCTTTTAAGACCATTTTTCAATTTTATCCTAACATCAGGAGTGGACAATGTTAGTCTTATTTATCATATAGTTTACACCTCACCTAAATAAAGCAAACGACTCAGGTTGGTCCTTGGCTCCAAGGAGGATTTGTGCCATATGATAAAACATAACTTGAGAATTGTTTAACCCAACAGCAATGTGCCATCAGTCAGGGGCTCAATTTACACTTGTAACGTATGATAAAACATAATTTGGAAACATGAAGTTCCTTTCTAACATTTGTGGATATGTATATTTTTAATTTGTTTAATTGACATGCACTCCCTTGTCACCTATTGAATTTCTTAAATTTGTTTAATTGACAGGAACTTCACATCAATGGAAATTCAATTGGTGATGAGGGAGTGCGTGCTTTAATTTCAGGTTTATCTTCACGTAAAGGTATGCCATATGATGCCATTCTACGTTCTTTCTGTTGTTGAAGTTTGAGCTTGATCAAATTCACATGCACGATATTTTATGCATTTGCCATTCGTTAACTTCAACAACATTCTTACTTCCTTGTAGGAAAACTTGCACTTCTTGACATTGGCAACAACTCAATCACTGCCAAGGGTGTCTTTCATGTTGCCGAATTCATTAAGAGAACTAAGAGCTTGGTTTTATTGAATTTGTTCATGAATGATATTGGTGATGAGGTAACGGGCAGAGCTTCTAAAATTACTCTTAGTATTTAACTTTTATAATATTTAAGTATTCTGTTAAGTTGTTGAATTTCAGTATTATGGGCTTCTTCCCTTGAGGAAGTTACATGAATAACACAAAATACCAAATCCAAGGTAGCATGATACTTGAACTTCTCTCTTAATCAAAGACAAAAAGAGAAAAAAAACCATGGAGATCCAAATGCATCTGAAAGCCACTGCTATCTGTGAGGGGCAGTCTATGGGATGAGCCATAGTGAATAAATCAGTTCTTTTTTCCCTTTTGTGGGTTGGTTTTTTGTATGCCTGCATATTCTTTCATTTTTCTCCATGAAAGTTTGGTTCCTTATCTGAAAAAAAACTTGTAAAGAGTGAAAATCATAAAACAAACCAATAGAAATGCAAAGTAGCCTATAAAGATTGAATAAAAGCACCCCAATTAATACAAATATCCCAAGCAGAATAAGACTCAAATAAATTAGAAAAAGGACACTAATGAGAAACCTTAAACTTAACAATATCAAATCATTCCATCAATGATTTACTTTTACCTTAAAAAATTATTTGGTTTCACTCAAACTGTAATAAGATAAAATGGATTTGACAGCGTTGACCCATAAAAGATGAGCTTGAGAAGATAAGGACGACCCATGAAGAAGCTGAAATAAATTATTTCTGGAATCCTTCGAGAATACCCAATCAAGATTAAAAATAGTAAACGAAGTGAACCAGCTTGATCACAAGTTTGTGAGGATGAAATCCAGGAATTTTTTATAGATGCATGTTTCCCGCTGGAGAGATTGTATAGCCTTGAGATTATCATCTAGAAAGGGTCACCACCACAAGTTGGTGGGAAAATCCTTGCCCACTGATTTCTTGCTACTTATATTTGATAATTGACTTCCAAAGACCTTTGGAGGTTTTATTATTATTATTTTTAATGAAATATCGAACTTTCAATGAGAAAAAGATGAAAGAATACAAGGATATACAAAAATAAAGCTCACAAAAAGGAGCCAAACTAAAAAAGGGCTCTAATCAAGTAAAATAAGACCCAGAGGATAATTACAAAATAACTTCGTTATCAAAGCCTGCAAAGGAATATGAAAGCTAACAAGGTACCAAACATTATCTGAAGGTCTCTCCAACCCTCTAATAATGTGTGAGACCTCCAATTATAAAAACGTACGAAAGAAGGGGCAAAATGGTCAAGGAGGACCAAACTAAGAAAGATAACAGAAATGGAGTGGGGACCAGCAAAGAATGATCGTGGGAACGTGTTATAGGCGTACGGGAGCAGAGCAAATGTCCTTATTAGGAGGATGGTTGTCTTTTTCGTTTTCTTTATGCATTTTGTGAGATTTAGAAAGTTTGTGTGCTTTCTGTGGAGAGGTACCAGGGCTCTCGAATCCCCTGTGTGTTAGTGTGTTTTCTTCTTTCGTTGTAATCGTTGAACCTTGGTTTTCGGGCTGTTTTCACTGTTCATCTCAATAGAACAGTGCTCCATTTGCCTATTGTGTGTGTTCTTTGGTTGTGTTTCGTTTTTCTTGCAGGAAAGCAGTTTGGATACCCAACATAATGCTATCATTTTGTTCTTCTCATAG

mRNA sequence

ATGACTTACGAGGAACTGAAGAGCATACCTTGGGCATCCATGAGTATGGAACTTCACATCAATGGAAATTCAATTGGTGATGAGGGAGTGCGTGCTTTAATTTCAGGTTTATCTTCACGTAAAGGAAAACTTGCACTTCTTGACATTGGCAACAACTCAATCACTGCCAAGGGTGTCTTTCATGTTGCCGAATTCATTAAGAGAACTAAGAGCTTGGTTTTATTGAATTTGTTCATGAATGATATTGGTGATGAGGAAAGCAGTTTGGATACCCAACATAATGCTATCATTTTGTTCTTCTCATAG

Coding sequence (CDS)

ATGACTTACGAGGAACTGAAGAGCATACCTTGGGCATCCATGAGTATGGAACTTCACATCAATGGAAATTCAATTGGTGATGAGGGAGTGCGTGCTTTAATTTCAGGTTTATCTTCACGTAAAGGAAAACTTGCACTTCTTGACATTGGCAACAACTCAATCACTGCCAAGGGTGTCTTTCATGTTGCCGAATTCATTAAGAGAACTAAGAGCTTGGTTTTATTGAATTTGTTCATGAATGATATTGGTGATGAGGAAAGCAGTTTGGATACCCAACATAATGCTATCATTTTGTTCTTCTCATAG

Protein sequence

MTYEELKSIPWASMSMELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLNLFMNDIGDEESSLDTQHNAIILFFS
Homology
[Close]
BLAST of Lag0005405 vs. NCBI nr
Match: XP_022999141.1 (NLR family CARD domain-containing protein 3 [Cucurbita maxima])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 66/69 (95.65%), Postives = 69/69 (100.00%), Query Frame = 0 

Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           ELH+NGNS+GDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEF+KRTKSLVLLN
Sbjct: 348 ELHMNGNSVGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFMKRTKSLVLLN 407

Query: 77  LFMNDIGDE 86
           LFMNDIGDE
Sbjct: 408 LFMNDIGDE 416

[Close]
BLAST of Lag0005405 vs. NCBI nr
Match: KAG6599019.1 (NLR family CARD domain-containing protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 66/69 (95.65%), Postives = 69/69 (100.00%), Query Frame = 0 

Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           ELH+NGNS+GDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEF+KRTKSLVLLN
Sbjct: 348 ELHMNGNSVGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFMKRTKSLVLLN 407

Query: 77  LFMNDIGDE 86
           LFMNDIGDE
Sbjct: 408 LFMNDIGDE 416

[Close]
BLAST of Lag0005405 vs. NCBI nr
Match: XP_023520466.1 (NLR family CARD domain-containing protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 66/69 (95.65%), Postives = 69/69 (100.00%), Query Frame = 0 

Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           ELH+NGNS+GDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEF+KRTKSLVLLN
Sbjct: 348 ELHMNGNSVGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFMKRTKSLVLLN 407

Query: 77  LFMNDIGDE 86
           LFMNDIGDE
Sbjct: 408 LFMNDIGDE 416

[Close]
BLAST of Lag0005405 vs. NCBI nr
Match: KAG7029980.1 (NLR family CARD domain-containing protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 66/69 (95.65%), Postives = 69/69 (100.00%), Query Frame = 0 

Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           ELH+NGNS+GDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEF+KRTKSLVLLN
Sbjct: 396 ELHMNGNSVGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFMKRTKSLVLLN 455

Query: 77  LFMNDIGDE 86
           LFMNDIGDE
Sbjct: 456 LFMNDIGDE 464

[Close]
BLAST of Lag0005405 vs. NCBI nr
Match: XP_022946525.1 (NLR family CARD domain-containing protein 3 [Cucurbita moschata])

HSP 1 Score: 133.3 bits (334), Expect = 1.2e-27
Identity = 66/69 (95.65%), Postives = 69/69 (100.00%), Query Frame = 0 

Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           ELH+NGNS+GDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEF+KRTKSLVLLN
Sbjct: 348 ELHMNGNSVGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFMKRTKSLVLLN 407

Query: 77  LFMNDIGDE 86
           LFMNDIGDE
Sbjct: 408 LFMNDIGDE 416

[Close]
BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match: A6QLE5 (NACHT, LRR and PYD domains-containing protein 3 OS=Bos taurus OX=9913 GN=NLRP3 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 6.7e-05
Identity = 25/67 (37.31%), Postives = 41/67 (61.19%), Query Frame = 0 

Query: 18  LHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLNL 77
           L++ GN++GD GV+ L  GL  R  KL +L++ N S+T+   + ++  +   +SL  L L
Sbjct: 912 LYLQGNALGDMGVKLLCEGLLHRNCKLQVLELDNCSLTSHCCWDLSTLLTSNQSLRKLCL 971

Query: 78  FMNDIGD 85
             ND+GD
Sbjct: 972 GNNDLGD 978

[Close]
BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match: Q96P20 (NACHT, LRR and PYD domains-containing protein 3 OS=Homo sapiens OX=9606 GN=NLRP3 PE=1 SV=3)

HSP 1 Score: 46.6 bits (109), Expect = 1.9e-04
Identity = 22/67 (32.84%), Postives = 43/67 (64.18%), Query Frame = 0 

Query: 18  LHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLNL 77
           L++ GN++GD+G++ L  GL     KL +L++ N ++T+   + ++  +  ++SL  L+L
Sbjct: 917 LYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSL 976

Query: 78  FMNDIGD 85
             ND+GD
Sbjct: 977 GNNDLGD 983

[Close]
BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match: B0FPE9 (NACHT, LRR and PYD domains-containing protein 3 OS=Macaca mulatta OX=9544 GN=NLRP3 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 1.9e-04
Identity = 22/67 (32.84%), Postives = 43/67 (64.18%), Query Frame = 0 

Query: 18  LHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLNL 77
           L++ GN++GD+G++ L  GL     KL +L++ N ++T+   + ++  +  ++SL  L+L
Sbjct: 916 LYLRGNTLGDKGIKLLCEGLLHPDCKLQVLELDNCNLTSHCCWDLSTLLTSSQSLRKLSL 975

Query: 78  FMNDIGD 85
             ND+GD
Sbjct: 976 GNNDLGD 982

[Close]
BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match: A6H639 (Dynein regulatory complex subunit 5 OS=Mus musculus OX=10090 GN=Tcte1 PE=1 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 7.4e-04
Identity = 27/69 (39.13%), Postives = 38/69 (55.07%), Query Frame = 0 

Query: 17  ELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIKRTKSLVLLN 76
           EL ++ N IGD G RA    LS    +L +L++ NN + A G   +A  +    +LV LN
Sbjct: 309 ELDLSHNLIGDRGARAAAKLLS--HSRLRVLNLANNQLQAPGAQSLAHALAHNTNLVFLN 368

Query: 77  LFMNDIGDE 86
           L +N I DE
Sbjct: 369 LRLNCIEDE 375

[Close]
BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match: O75864 (Protein phosphatase 1 regulatory subunit 37 OS=Homo sapiens OX=9606 GN=PPP1R37 PE=1 SV=4)

HSP 1 Score: 44.3 bits (103), Expect = 9.6e-04
Identity = 27/75 (36.00%), Postives = 42/75 (56.00%), Query Frame = 0 

Query: 8   SIPWASMSMELHINGNSIGDEGVRALISGLSSRKGKLALLDIGNNSITAKGVFHVAEFIK 67
           ++P       L++  N IG+EGVR L +GL S +  L  L + +  +T +G   VAEFI 
Sbjct: 328 TLPHTQSLETLNLGHNPIGNEGVRHLKNGLISNRSVLR-LGLASTKLTCEGAVAVAEFIA 387

Query: 68  RTKSLVLLNLFMNDI 83
            +  L+ L+L  N+I
Sbjct: 388 ESPRLLRLDLRENEI 401

The following BLAST results are available for this feature:
BLAST of Lag0005405 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022999141.11.2e-2795.65NLR family CARD domain-containing protein 3 [Cucurbita maxima][more]
KAG6599019.11.2e-2795.65NLR family CARD domain-containing protein 3, partial [Cucurbita argyrosperma sub... [more]
XP_023520466.11.2e-2795.65NLR family CARD domain-containing protein 3 [Cucurbita pepo subsp. pepo][more]
KAG7029980.11.2e-2795.65NLR family CARD domain-containing protein 3, partial [Cucurbita argyrosperma sub... [more]
XP_022946525.11.2e-2795.65NLR family CARD domain-containing protein 3 [Cucurbita moschata][more]
BLAST of Lag0005405 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
A6QLE56.7e-0537.31NACHT, LRR and PYD domains-containing protein 3 OS=Bos taurus OX=9913 GN=NLRP3 P... [more]
Q96P201.9e-0432.84NACHT, LRR and PYD domains-containing protein 3 OS=Homo sapiens OX=9606 GN=NLRP3... [more]
B0FPE91.9e-0432.84NACHT, LRR and PYD domains-containing protein 3 OS=Macaca mulatta OX=9544 GN=NLR... [more]
A6H6397.4e-0439.13Dynein regulatory complex subunit 5 OS=Mus musculus OX=10090 GN=Tcte1 PE=1 SV=1[more]
O758649.6e-0436.00Protein phosphatase 1 regulatory subunit 37 OS=Homo sapiens OX=9606 GN=PPP1R37 P... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00368LRR_RI_2coord: 41..68
e-value: 4.3
score: 12.7
coord: 13..39
e-value: 1.3
score: 16.3
NoneNo IPR availablePANTHERPTHR24106:SF267LEUCINE RICH REPEAT FAMILY PROTEINcoord: 16..85
NoneNo IPR availablePANTHERPTHR24106NACHT, LRR AND CARD DOMAINS-CONTAININGcoord: 16..85
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 17..85
IPR001611Leucine-rich repeatPFAMPF13516LRR_6coord: 72..85
e-value: 1.5
score: 9.1
coord: 17..33
e-value: 0.0037
score: 17.2
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 6..96
e-value: 5.0E-15
score: 57.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005405.1Lag0005405.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding
molecular_function GO:0016740 transferase activity