Lag0005221 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0005221 vs. NCBI nr
Match: XP_038885582.1 (gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 258.5 bits (659), Expect = 3.8e-65
Identity = 123/155 (79.35%), Postives = 135/155 (87.10%), Query Frame = 0

Query: 1   MAD-DQELPPPSQIVPLNFSSIQTIPESHLWTGSNELVPAIDLDEIFSLPLIDLLDDDAS 60
           MAD DQELPPPSQ VPL+FSSIQT+PESHLW  SNEL  A++LDE  SLP+IDLLD+ AS
Sbjct: 1   MADHDQELPPPSQFVPLDFSSIQTVPESHLWPDSNELSLAVELDERVSLPIIDLLDNGAS 60

Query: 61  EHLGRACEQWGMFQLINHGVPKTLIAETEGEAQRLFALPARQKMKTLQSPSHFTGYGMAG 120
           E LGRACE+WGMFQLINHGVPKTLI E E E +RLF LPARQKMK LQSPS  TGYGMAG
Sbjct: 61  ELLGRACEEWGMFQLINHGVPKTLIDEAEEETRRLFTLPARQKMKALQSPSSITGYGMAG 120

Query: 121 ISKYYSKYMWHEGFTILGSPVDDAKKLWPSDFKRF 155
           ISKY+ K MWHEGFT+LGSPVDDAKKLWP+D+KRF
Sbjct: 121 ISKYHPKLMWHEGFTVLGSPVDDAKKLWPNDYKRF 155

BLAST of Lag0005221 vs. NCBI nr
Match: AAF80661.1 (putative gibberellin 3 beta hydroxylase [Citrullus lanatus subsp. vulgaris])

HSP 1 Score: 246.5 bits (628), Expect = 1.5e-61
Identity = 118/152 (77.63%), Postives = 131/152 (86.18%), Query Frame = 0

Query: 4   DQELPPPSQIVPLNFSSIQTIPESHLWTGSNELVPAIDLDEIFSLPLIDLLDDDASEHLG 63
           DQELPPPSQ+VPL+F+SIQT+PESHLW  S EL PAI LDE  SLP+ID LDD ASE LG
Sbjct: 3   DQELPPPSQLVPLDFNSIQTVPESHLWPNSIELSPAIKLDERISLPIIDFLDDGASELLG 62

Query: 64  RACEQWGMFQLINHGVPKTLIAETEGEAQRLFALPARQKMKTLQSPS-HFTGYGMAGISK 123
           RACEQWGMFQLINHGVPKTLI E E E +RLF+LPARQKMKTL++ S   TGYGMAGISK
Sbjct: 63  RACEQWGMFQLINHGVPKTLIDEAEEETRRLFSLPARQKMKTLRTDSLTATGYGMAGISK 122

Query: 124 YYSKYMWHEGFTILGSPVDDAKKLWPSDFKRF 155
           Y+ K MWHEGFT+ GSPVDDAKKLWP+D+KRF
Sbjct: 123 YHQKLMWHEGFTMSGSPVDDAKKLWPNDYKRF 154

BLAST of Lag0005221 vs. NCBI nr
Match: KAA0064969.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 237.7 bits (605), Expect = 6.9e-59
Identity = 111/155 (71.61%), Postives = 130/155 (83.87%), Query Frame = 0

Query: 4   DQELPPPSQIVPLNFSSIQT-IPESHLWTGSNELVPAIDLDEIFSLPLIDLLDDDASEHL 63
           DQE+PPPSQ+VPLNF+S+QT +PESHLW    EL  A+ LD+  SLPLIDL++DDASE L
Sbjct: 3   DQEIPPPSQLVPLNFASVQTHVPESHLWPNIKELSAAVKLDKRVSLPLIDLVNDDASELL 62

Query: 64  GRACEQWGMFQLINHGVPKTLIAETEGEAQRLFALPARQKMKTLQSPSHF--TGYGMAGI 123
           GRACE+WGMFQLINHGV +TLI E E E QRLF LPA QKMKTL+SP     TGYGMAGI
Sbjct: 63  GRACEEWGMFQLINHGVSQTLIEEAEEETQRLFTLPAAQKMKTLRSPGSLTATGYGMAGI 122

Query: 124 SKYYSKYMWHEGFTILGSPVDDAKKLWPSDFKRFW 156
           SKY+ K MWHEGFTI+GSP+++AKKLWP+D+KRFW
Sbjct: 123 SKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRFW 157

BLAST of Lag0005221 vs. NCBI nr
Match: NP_001275523.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >KAE8652189.1 hypothetical protein Csa_021993 [Cucumis sativus] >CCG14217.1 GA 3-oxidase [Cucumis sativus])

HSP 1 Score: 235.0 bits (598), Expect = 4.5e-58
Identity = 109/153 (71.24%), Postives = 128/153 (83.66%), Query Frame = 0

Query: 4   DQELPPPSQIVPLNFSSIQTIPESHLWTGSNELVPAIDLDEIFSLPLIDLLDDDASEHLG 63
           DQE+PPPSQ+VPL+F+S+QT+PESHLW  + EL  AI LD+  SLPLIDL+ D ASE LG
Sbjct: 3   DQEIPPPSQLVPLDFTSVQTVPESHLWPNATELSAAIKLDKRVSLPLIDLVSDGASELLG 62

Query: 64  RACEQWGMFQLINHGVPKTLIAETEGEAQRLFALPARQKMKTLQSPSHF--TGYGMAGIS 123
           RACE+WGMFQLINHG+ KTLI E E E +RLF LPA QKMKTL+SP     TGYGMAGIS
Sbjct: 63  RACEEWGMFQLINHGISKTLIEEAEEETRRLFTLPATQKMKTLRSPGSLTATGYGMAGIS 122

Query: 124 KYYSKYMWHEGFTILGSPVDDAKKLWPSDFKRF 155
           KY+ K MWHEGFTILGSP+++AKKLWP+D+KRF
Sbjct: 123 KYHPKLMWHEGFTILGSPINEAKKLWPNDYKRF 155

BLAST of Lag0005221 vs. NCBI nr
Match: XP_008445138.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 231.9 bits (590), Expect = 3.8e-57
Identity = 109/154 (70.78%), Postives = 129/154 (83.77%), Query Frame = 0

Query: 4   DQELPPPSQIVPLNFSSIQT-IPESHLWTGSNELVPAIDLDEIFSLPLIDLLDDDASEHL 63
           DQE+PPPSQ+VPLNF+S+QT +PESHLW    EL  A+ LD+  SLPLIDL++DDASE L
Sbjct: 3   DQEIPPPSQLVPLNFASVQTHVPESHLWPNVKELSAAVKLDKRVSLPLIDLVNDDASELL 62

Query: 64  GRACEQWGMFQLINHGVPKTLIAETEGEAQRLFALPARQKMKTLQSPSHF--TGYGMAGI 123
           GRACE+WGMFQLINHGV +TLI E E E +RLF LPA QKMKTL+SP     TGYGMAGI
Sbjct: 63  GRACEEWGMFQLINHGVSQTLIEEAEEETRRLFTLPAAQKMKTLRSPGSLTATGYGMAGI 122

Query: 124 SKYYSKYMWHEGFTILGSPVDDAKKLWPSDFKRF 155
           SKY+ K MWHEGFTI+GSP+++AKKLWP+D+KRF
Sbjct: 123 SKYHPKLMWHEGFTIIGSPINEAKKLWPNDYKRF 156

BLAST of Lag0005221 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.5e-32
Identity = 63/143 (44.06%), Postives = 100/143 (69.93%), Query Frame = 0

Query: 17  NFSSIQTIPESHLWTGSNELVPAIDLDEIF-----SLPLIDLLDDDASEHLGRACEQWGM 76
           +F+S+Q +PES+ WT  ++    ID + I      ++P+IDL D +AS+ +G AC+ WG+
Sbjct: 21  DFNSLQELPESYNWTHLDDHT-LIDSNNIMKESTTTVPVIDLNDPNASKLIGLACKTWGV 80

Query: 77  FQLINHGVPKTLIAETEGEAQRLFALPARQKMKTLQSPSHFTGYGMAGISKYYSKYMWHE 136
           +Q++NHG+P +L+ + +   Q LF+LP+ QK K  +SP   +GYG+A IS ++ K MW+E
Sbjct: 81  YQVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVSGYGIARISSFFPKLMWYE 140

Query: 137 GFTILGSPVDDAKKLWPSDFKRF 155
           GFTI+GSP+D  ++LWP D+ RF
Sbjct: 141 GFTIVGSPLDHFRELWPQDYTRF 162

BLAST of Lag0005221 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 124.8 bits (312), Expect = 8.6e-28
Identity = 60/146 (41.10%), Postives = 93/146 (63.70%), Query Frame = 0

Query: 10  PSQIVPLNFSSIQTIPESHLWTGSNELV-------PAIDLDEIFSLPLIDLLDDDASEHL 69
           P   +P +F+S++ +P+S+ WT  ++L+       PA       ++PLIDL   DA+  +
Sbjct: 17  PHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATG----ENIPLIDLDHPDATNQI 76

Query: 70  GRACEQWGMFQLINHGVPKTLIAETEGEAQRLFALPARQKMKTLQSPSHFTGYGMAGISK 129
           G AC  WG FQ+ NHGVP  L+ + E     LF LP ++K+K+ +S +  +GYG+A I+ 
Sbjct: 77  GHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGYGVARIAS 136

Query: 130 YYSKYMWHEGFTILGSPVDDAKKLWP 149
           +++K MW EGFTI GSP++D +KLWP
Sbjct: 137 FFNKQMWSEGFTITGSPLNDFRKLWP 157

BLAST of Lag0005221 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-27
Identity = 60/146 (41.10%), Postives = 91/146 (62.33%), Query Frame = 0

Query: 10  PSQIVPLNFSSIQTIPESHLWTGSNELV-PAIDLDEIFSLPLIDLLDDDASEHLGRACEQ 69
           P  I   N    +++P+S+ WT  ++L+  A   DE  +LPLIDL D   +  +G AC  
Sbjct: 13  PIHIPLSNPPDFKSLPDSYTWTPKDDLLFSASASDE--TLPLIDLSDIHVATLVGHACTT 72

Query: 70  WGMFQLINHGVPKTLIAETEGEAQRLFALPARQKMKTLQSPSHFTGYGMAGISKYYSKYM 129
           WG FQ+ NHGVP  L+ + E     LF LP ++K+K  +S +  +GYG+A I+ +++K M
Sbjct: 73  WGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGVARIASFFNKKM 132

Query: 130 WHEGFTILGSPVDDAKKLWPSDFKRF 155
           W EGFT++GSP+ D +KLWPS   ++
Sbjct: 133 WSEGFTVIGSPLHDFRKLWPSHHLKY 156

BLAST of Lag0005221 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 5.2e-25
Identity = 52/144 (36.11%), Postives = 88/144 (61.11%), Query Frame = 0

Query: 12  QIVPLNFSSIQTIPESHLWTGSNELVPAIDLDEIFSLPLIDLLDDDASEHLGRACEQWGM 71
           +I+PL+F++ +T+P+SH+W+       +        +P+I L + +    L +ACE+WG+
Sbjct: 17  RIIPLDFTNTKTLPDSHVWSKPEPETTS------GPIPVISLSNPEEHGLLRQACEEWGV 76

Query: 72  FQLINHGVPKTLIAETEGEAQRLFALPARQKMKTLQSPSHFTGYGMAGISKYYSKYMWHE 131
           F + +HGV  +L+   + + +RLF+LP  +K+  ++SP   TGYG+  IS +Y K MW E
Sbjct: 77  FHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGVVRISMFYDKLMWSE 136

Query: 132 GFTILGSPV-DDAKKLWPSDFKRF 155
           GF+++GS +   A  LWP D   F
Sbjct: 137 GFSVMGSSLRRHATLLWPDDHAEF 154

BLAST of Lag0005221 vs. ExPASy Swiss-Prot
Match: Q3I409 (Gibberellin 3-beta-dioxygenase 2-3 OS=Triticum aestivum OX=4565 GN=GA3ox2-3 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 6.4e-23
Identity = 55/148 (37.16%), Postives = 85/148 (57.43%), Query Frame = 0

Query: 17  NFSSIQTIPESHLWTGSNELVPAIDLD----EIFSLPLIDLLDDDASEHLGRACEQWGMF 76
           +F + + +PE+H W G ++  P +D      E  ++P++D+ D  A+E +G A + WG F
Sbjct: 15  DFRAARRVPETHAWPGLHD-HPVVDGSGAGGEPDAVPVVDMRDPFAAEAVGLAAQDWGAF 74

Query: 77  QLINHGVPKTLIAETEGEAQRLFALPARQKMKTLQSPSHFTGYGMAGISKYYSKYMWHEG 136
            L+ HGVP  L+   E     +FALPA +KM+ ++ P    GYG   IS ++SK MW EG
Sbjct: 75  LLVGHGVPLDLLVRVEAAIAGMFALPASEKMRAVRRPGDSCGYGSPPISSFFSKCMWSEG 134

Query: 137 FTILGSPVD---DAKKLWPS---DFKRF 155
           +T   SP +   D +KLWP    D++ F
Sbjct: 135 YTF--SPANLRSDLRKLWPKAGHDYRHF 159