Lag0004945 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0004945 vs. NCBI nr
Match: KAG6598715.1 (hypothetical protein SDJN03_08493, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 360.9 bits (925), Expect = 7.6e-96
Identity = 168/217 (77.42%), Postives = 191/217 (88.02%), Query Frame = 0

Query: 1   MASEGKIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEG 60
           M SE  IQ KP+ILCLHGFRTSGAILKK +EKWPTSVL+QFDFHF+DAPFPS G SEVEG
Sbjct: 1   MGSESNIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLDQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQE 120
           + DPPYFEWF+F EDT  + N++ECLQFI+N+MLEHGPFDGLLGFSQGAAL+A LPG Q 
Sbjct: 61  LHDPPYFEWFQFHEDTADFANVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 KGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLLE 180
           KG+ALT+VPKIKFVIIISG K  SPS+ A++AY+SSI CPSLHFLSEEDF MPSGL+LLE
Sbjct: 121 KGVALTRVPKIKFVIIISGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMPSGLQLLE 180

Query: 181 SFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISKM 218
           SF EP VINH KGHTVP+LD+K LK+MENFI RISK+
Sbjct: 181 SFVEPFVINHLKGHTVPKLDQKSLKIMENFIDRISKL 217

BLAST of Lag0004945 vs. NCBI nr
Match: XP_022131300.1 (dihydrofolate reductase-like [Momordica charantia])

HSP 1 Score: 360.5 bits (924), Expect = 9.9e-96
Identity = 171/216 (79.17%), Postives = 190/216 (87.96%), Query Frame = 0

Query: 1   MASEGKIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEG 60
           M SE KIQ KP+ LCLHGFRTSG ILKKQ+ KWP+SVL+ FD HFLDAPFP+ G S+VEG
Sbjct: 35  MGSEAKIQRKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEG 94

Query: 61  IFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQE 120
           IFDPPYFEWF+FS D   Y N  ECL FI++YML+HGPFDG LGFSQGA LSAALPGLQE
Sbjct: 95  IFDPPYFEWFQFSADMMEYQNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQE 154

Query: 121 KGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLLE 180
           KGIALTKVPKIKFV+I+SG+KFRSPS+ AEKAYS SI CPSLHFL +EDFLMP GLKLLE
Sbjct: 155 KGIALTKVPKIKFVVIVSGAKFRSPSL-AEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLE 214

Query: 181 SFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISK 217
           S+ EPLVINHPKGHTVPRLDEKGLKVME+FI+RIS+
Sbjct: 215 SYIEPLVINHPKGHTVPRLDEKGLKVMESFIERISQ 249

BLAST of Lag0004945 vs. NCBI nr
Match: XP_022997265.1 (dihydrofolate reductase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 359.0 bits (920), Expect = 2.9e-95
Identity = 166/217 (76.50%), Postives = 191/217 (88.02%), Query Frame = 0

Query: 1   MASEGKIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEG 60
           M S+ +IQ KP+ILCLHGFRTSGAILKK +EKWPTSVLNQFDFHF+DAPFPS G SEVEG
Sbjct: 1   MGSKSRIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQE 120
           + DPPY+EWF+F EDT  + N++ECLQFI+N+MLEHGPFDGLLGFSQGAAL+A LPG Q 
Sbjct: 61  LHDPPYYEWFQFHEDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 KGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLLE 180
           KG+ALT+VPKIKFVIII+G K  SPS+ A++AY+SSI CPSLHFLSEEDF MPSGL+LLE
Sbjct: 121 KGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMPSGLQLLE 180

Query: 181 SFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISKM 218
           SF EP VINH KGH VPRLD+K LK+MENFI RISK+
Sbjct: 181 SFVEPFVINHLKGHAVPRLDQKSLKIMENFIDRISKL 217

BLAST of Lag0004945 vs. NCBI nr
Match: XP_022961840.1 (dihydrofolate reductase-like [Cucurbita moschata])

HSP 1 Score: 357.5 bits (916), Expect = 8.4e-95
Identity = 167/217 (76.96%), Postives = 190/217 (87.56%), Query Frame = 0

Query: 1   MASEGKIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEG 60
           M SE  IQ KP+ILCLHGFRTSGAILKK +EKWPTSVL+QFDFHF+DAPFPS G SEVEG
Sbjct: 1   MGSESNIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLDQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQE 120
           + DPPYFEWF+F EDT  + N++ECLQFI+N+MLEHGPFDGLLGFSQGAAL+A LPG Q 
Sbjct: 61  LHDPPYFEWFQFHEDTADFANVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 KGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLLE 180
           KG+ALT+VPKIKFVIIISG K  SPS+ A++A +SSI CPSLHFLSEEDF MPSGL+LLE
Sbjct: 121 KGVALTRVPKIKFVIIISGVKLESPSLDAQEASASSIACPSLHFLSEEDFFMPSGLQLLE 180

Query: 181 SFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISKM 218
           SF EP VINH KGHTVP+LD+K LK+MENFI RISK+
Sbjct: 181 SFVEPFVINHLKGHTVPKLDQKSLKIMENFIDRISKL 217

BLAST of Lag0004945 vs. NCBI nr
Match: XP_022997264.1 (esterase CG5412-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 354.4 bits (908), Expect = 7.1e-94
Identity = 166/218 (76.15%), Postives = 191/218 (87.61%), Query Frame = 0

Query: 1   MASEGKIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEG 60
           M S+ +IQ KP+ILCLHGFRTSGAILKK +EKWPTSVLNQFDFHF+DAPFPS G SEVEG
Sbjct: 1   MGSKSRIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQE 120
           + DPPY+EWF+F EDT  + N++ECLQFI+N+MLEHGPFDGLLGFSQGAAL+A LPG Q 
Sbjct: 61  LHDPPYYEWFQFHEDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 K-GIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLL 180
           K G+ALT+VPKIKFVIII+G K  SPS+ A++AY+SSI CPSLHFLSEEDF MPSGL+LL
Sbjct: 121 KQGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMPSGLQLL 180

Query: 181 ESFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISKM 218
           ESF EP VINH KGH VPRLD+K LK+MENFI RISK+
Sbjct: 181 ESFVEPFVINHLKGHAVPRLDQKSLKIMENFIDRISKL 218

BLAST of Lag0004945 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 96.3 bits (238), Expect = 4.6e-19
Identity = 64/208 (30.77%), Postives = 98/208 (47.12%), Query Frame = 0

Query: 9   TKP-KILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSE---------- 68
           +KP K+LCLHG+  SG +  K++      +    + HF   P  +   ++          
Sbjct: 2   SKPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRL 61

Query: 69  --VEGIFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAAL 128
             + G  +   F WF   +  N+Y +  E L+ I+ YM E GPFDGL+GFSQGA + A L
Sbjct: 62  AALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAML 121

Query: 129 PGLQEKG---IALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLM 188
             + + G       + P  KFV+ + G  FR+     +  Y+  +  PSLH     D L+
Sbjct: 122 AQMLQPGQPPNPYVQHPPFKFVVFVGG--FRAEKPEFDHFYNPKLTTPSLHIAGTSDTLV 181

Query: 189 P--SGLKLLESFFEPLVINHPKGHTVPR 199
           P     +L+E      V+ HP  H VP+
Sbjct: 182 PLARSKQLVERCENAHVLLHPGQHIVPQ 207

BLAST of Lag0004945 vs. ExPASy Swiss-Prot
Match: Q503Y4 (Esterase OVCA2 OS=Danio rerio OX=7955 GN=ovca2 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.1e-16
Identity = 64/208 (30.77%), Postives = 101/208 (48.56%), Query Frame = 0

Query: 12  KILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPF-------PSSGT---SEVEGI 71
           +ILC+HG+R +G   +++       +  Q +  F+ AP         + GT   S+   +
Sbjct: 7   RILCIHGYRQNGNSFREKTGALRKLLKKQVELVFISAPHQVPAIQEENCGTNQQSQTVSV 66

Query: 72  FDPPYFEW---------FRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALS 131
            D     W         F   +D  S + L+E ++ +   + + GPF G+LGFSQGAAL 
Sbjct: 67  GDEDQRGWWFSDVQARSFNAKQDCESSLGLEESIEAVKAALKDLGPFSGILGFSQGAALV 126

Query: 132 AALPGLQEKGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSS-IKCPSLHFLSEEDFL 191
           A L  LQE+   L      +F I+++G  FRS  +  ++ Y    I  PSLH   ++D +
Sbjct: 127 AMLCALQEQ--KLEPDFNFRFAILVAG--FRSACLEHQRFYEGPVITIPSLHVFGQDDRV 186

Query: 192 MPSGLK--LLESFFEPLVINHPKGHTVP 198
           +P  +   LL +F    V+ HP GH VP
Sbjct: 187 IPEEMSRDLLPAFDGAQVLLHPGGHFVP 210

BLAST of Lag0004945 vs. ExPASy Swiss-Prot
Match: O13897 (Uncharacterized hydrolase C22A12.06c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC22A12.06c PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-15
Identity = 59/211 (27.96%), Postives = 96/211 (45.50%), Query Frame = 0

Query: 6   KIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFP---------SSGTS 65
           K  TK KILC+HG+  SG +   ++      + +  DF+F   P            SG  
Sbjct: 2   KSATKSKILCIHGYAESGELFSVKLRALRERMADSVDFYFPTGPIELDKAKDELNGSGFD 61

Query: 66  EVEGIFDPPYFE----WFRFSE--DTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAA 125
            +  +F          W+R +E  DT      K   +++ +Y+ EHGPFDG+LGFSQG  
Sbjct: 62  ALSTVFSSSPASHRRGWWRINEYADTKQLEPTK-AFEYLASYIKEHGPFDGILGFSQGTN 121

Query: 126 LSAALPGLQ--EKGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEE 185
           L+A L  L    K       P  +F +  SG  +  P +     +++ +  P+LH L + 
Sbjct: 122 LAANLAALVTIPKYQEYFSQPPFRFALFFSG--YFRPLLMDGAVHATKLDLPTLHLLGKY 181

Query: 186 DFLM--PSGLKLLESFFEPLVINHPKGHTVP 198
           D ++   +   L+ +  +  V+ HP  H +P
Sbjct: 182 DTVLSTETSTTLVRACKDAQVLFHPAAHQIP 209

BLAST of Lag0004945 vs. ExPASy Swiss-Prot
Match: Q61YZ4 (Esterase CBG03338 OS=Caenorhabditis briggsae OX=6238 GN=CBG03338 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 3.4e-14
Identity = 65/203 (32.02%), Postives = 96/203 (47.29%), Query Frame = 0

Query: 8   QTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLD-----APFPSSGTSEVEGIF 67
           Q K +ILCLHG+R      +++       V    DF F++     A    S TS      
Sbjct: 4   QPKLRILCLHGYRQCDQSFRQKTGSTRKLVKALADFEFVNGIHSVAVDEHSETSRAWWFS 63

Query: 68  DPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQEKG 127
           +      F   E T+  V   E +  +  ++ ++GPFDGLLGFSQGA++   L  + +  
Sbjct: 64  NADQMS-FSSREPTDVSVGFDESVNAVVKFIEDNGPFDGLLGFSQGASMVHLL--IAKAQ 123

Query: 128 IALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIK-CPSLHFLSEEDFLM--PSGLKLL 187
           +   K+P I+F I  SG  F S S   +   S  IK  PS+H   + D ++  P   KL 
Sbjct: 124 LGEIKLPGIRFAIFFSG--FLSLSSKHDTLTSLRIKDFPSMHVFGDSDEIVARPKSEKLA 183

Query: 188 ESF-FEPLVINHPKGHTVPRLDE 202
           + F  EP+ I H  GH VP + +
Sbjct: 184 DQFDMEPIRIAHEGGHLVPSMSK 201

BLAST of Lag0004945 vs. ExPASy Swiss-Prot
Match: Q18169 (Esterase C25G4.2 OS=Caenorhabditis elegans OX=6239 GN=C25G4.2 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 3.4e-14
Identity = 63/207 (30.43%), Postives = 97/207 (46.86%), Query Frame = 0

Query: 8   QTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEGI----FD 67
           Q K +ILCLHG+R      +++       V +  +F F++     +    V+      F 
Sbjct: 4   QPKLRILCLHGYRQCDQSFRQKTGSTRKLVKSLAEFEFVNGVHSVAVDEHVDSSRAWWFS 63

Query: 68  PPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQEKGI 127
                 F   E T   V  +E +  +  ++ E+GPFDGLLGFSQGA++   L  + +  +
Sbjct: 64  NNEAMSFSSRESTEVAVGFEESVAAVVKFIEENGPFDGLLGFSQGASMVHLL--IAKAQL 123

Query: 128 ALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIK-CPSLHFLSEEDFLM--PSGLKLLE 187
              K+P I+F I  SG  F S S   +      IK  PS+H   + D ++  P   K+ +
Sbjct: 124 GEIKLPGIRFAIFFSG--FLSLSSKHDSLTLLRIKEFPSMHVFGDADEIVARPKSEKMAD 183

Query: 188 SF-FEPLVINHPKGHTVPRLDEKGLKV 207
            F  EPL I H  GH VP + +   K+
Sbjct: 184 MFDVEPLRIAHDGGHVVPSMSKHKEKI 206

BLAST of Lag0004945 vs. ExPASy TrEMBL
Match: A0A6J1BPA7 (dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111004563 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.8e-96
Identity = 171/216 (79.17%), Postives = 190/216 (87.96%), Query Frame = 0

Query: 1   MASEGKIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEG 60
           M SE KIQ KP+ LCLHGFRTSG ILKKQ+ KWP+SVL+ FD HFLDAPFP+ G S+VEG
Sbjct: 35  MGSEAKIQRKPRFLCLHGFRTSGQILKKQVGKWPSSVLDNFDLHFLDAPFPAEGKSDVEG 94

Query: 61  IFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQE 120
           IFDPPYFEWF+FS D   Y N  ECL FI++YML+HGPFDG LGFSQGA LSAALPGLQE
Sbjct: 95  IFDPPYFEWFQFSADMMEYQNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQE 154

Query: 121 KGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLLE 180
           KGIALTKVPKIKFV+I+SG+KFRSPS+ AEKAYS SI CPSLHFL +EDFLMP GLKLLE
Sbjct: 155 KGIALTKVPKIKFVVIVSGAKFRSPSL-AEKAYSPSIGCPSLHFLGDEDFLMPHGLKLLE 214

Query: 181 SFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISK 217
           S+ EPLVINHPKGHTVPRLDEKGLKVME+FI+RIS+
Sbjct: 215 SYIEPLVINHPKGHTVPRLDEKGLKVMESFIERISQ 249

BLAST of Lag0004945 vs. ExPASy TrEMBL
Match: A0A6J1K941 (dihydrofolate reductase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492222 PE=4 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.4e-95
Identity = 166/217 (76.50%), Postives = 191/217 (88.02%), Query Frame = 0

Query: 1   MASEGKIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEG 60
           M S+ +IQ KP+ILCLHGFRTSGAILKK +EKWPTSVLNQFDFHF+DAPFPS G SEVEG
Sbjct: 1   MGSKSRIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQE 120
           + DPPY+EWF+F EDT  + N++ECLQFI+N+MLEHGPFDGLLGFSQGAAL+A LPG Q 
Sbjct: 61  LHDPPYYEWFQFHEDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 KGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLLE 180
           KG+ALT+VPKIKFVIII+G K  SPS+ A++AY+SSI CPSLHFLSEEDF MPSGL+LLE
Sbjct: 121 KGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMPSGLQLLE 180

Query: 181 SFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISKM 218
           SF EP VINH KGH VPRLD+K LK+MENFI RISK+
Sbjct: 181 SFVEPFVINHLKGHAVPRLDQKSLKIMENFIDRISKL 217

BLAST of Lag0004945 vs. ExPASy TrEMBL
Match: A0A6J1HDC8 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462485 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 4.1e-95
Identity = 167/217 (76.96%), Postives = 190/217 (87.56%), Query Frame = 0

Query: 1   MASEGKIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEG 60
           M SE  IQ KP+ILCLHGFRTSGAILKK +EKWPTSVL+QFDFHF+DAPFPS G SEVEG
Sbjct: 1   MGSESNIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLDQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQE 120
           + DPPYFEWF+F EDT  + N++ECLQFI+N+MLEHGPFDGLLGFSQGAAL+A LPG Q 
Sbjct: 61  LHDPPYFEWFQFHEDTADFANVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 KGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLLE 180
           KG+ALT+VPKIKFVIIISG K  SPS+ A++A +SSI CPSLHFLSEEDF MPSGL+LLE
Sbjct: 121 KGVALTRVPKIKFVIIISGVKLESPSLDAQEASASSIACPSLHFLSEEDFFMPSGLQLLE 180

Query: 181 SFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISKM 218
           SF EP VINH KGHTVP+LD+K LK+MENFI RISK+
Sbjct: 181 SFVEPFVINHLKGHTVPKLDQKSLKIMENFIDRISKL 217

BLAST of Lag0004945 vs. ExPASy TrEMBL
Match: A0A6J1KAZ1 (esterase CG5412-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492222 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.4e-94
Identity = 166/218 (76.15%), Postives = 191/218 (87.61%), Query Frame = 0

Query: 1   MASEGKIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEG 60
           M S+ +IQ KP+ILCLHGFRTSGAILKK +EKWPTSVLNQFDFHF+DAPFPS G SEVEG
Sbjct: 1   MGSKSRIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNQFDFHFMDAPFPSRGKSEVEG 60

Query: 61  IFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQE 120
           + DPPY+EWF+F EDT  + N++ECLQFI+N+MLEHGPFDGLLGFSQGAAL+A LPG Q 
Sbjct: 61  LHDPPYYEWFQFHEDTADFTNVEECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQA 120

Query: 121 K-GIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLL 180
           K G+ALT+VPKIKFVIII+G K  SPS+ A++AY+SSI CPSLHFLSEEDF MPSGL+LL
Sbjct: 121 KQGVALTRVPKIKFVIIINGVKLESPSLDAQEAYASSIACPSLHFLSEEDFFMPSGLQLL 180

Query: 181 ESFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISKM 218
           ESF EP VINH KGH VPRLD+K LK+MENFI RISK+
Sbjct: 181 ESFVEPFVINHLKGHAVPRLDQKSLKIMENFIDRISKL 218

BLAST of Lag0004945 vs. ExPASy TrEMBL
Match: A0A6J1BP70 (dihydrofolate reductase-like OS=Momordica charantia OX=3673 GN=LOC111004564 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.7e-93
Identity = 166/217 (76.50%), Postives = 189/217 (87.10%), Query Frame = 0

Query: 1   MASEGKIQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVEG 60
           M SEG+IQ KPK LCLHGFRTSG ILKKQ+ KWP+SVL+  D HF D PFPS+G SEVEG
Sbjct: 1   MGSEGEIQRKPKFLCLHGFRTSGEILKKQVGKWPSSVLDLLDLHFPDGPFPSAGRSEVEG 60

Query: 61  IFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQE 120
           IFDPP+FEWF+FS D   Y+N  ECL FI++YML++GPFDGLLGFSQGA LSAALPGLQE
Sbjct: 61  IFDPPFFEWFQFSADMTEYINFDECLSFIEDYMLKNGPFDGLLGFSQGAVLSAALPGLQE 120

Query: 121 KGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLLE 180
           KG+ALTKVPKIKFVII+SG+KF SP + AEKAYS S  CPSLHFL EEDFLMP+GL+LLE
Sbjct: 121 KGVALTKVPKIKFVIIVSGAKFLSP-LLAEKAYSPSFTCPSLHFLGEEDFLMPAGLELLE 180

Query: 181 SFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISKM 218
           SF +PLVINHPKGHTVPRLDEK LK+ME+FI++ISKM
Sbjct: 181 SFVDPLVINHPKGHTVPRLDEKSLKIMESFIEKISKM 216

BLAST of Lag0004945 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 274.6 bits (701), Expect = 6.7e-74
Identity = 129/218 (59.17%), Postives = 167/218 (76.61%), Query Frame = 0

Query: 1   MASEGK-IQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVE 60
           M SEG+ I  KP+ LCLHGFRTSG I+K Q+ KWP SV+++ D  FLDAPFP  G S+VE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GIFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQ 120
           GIFDPPY+EWF+F+++   Y N ++CL+++++ M++ GPFDGL+GFSQGA LS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 121 EKGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPSGLKLL 180
            KGIA  KVPKIKFVIII G+K +S  + AE AYSSS++  SLHFL E DFL P G +L+
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKL-AENAYSSSLETLSLHFLGETDFLKPYGTQLI 180

Query: 181 ESFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISKM 218
           ES+  P+V++HPKGHTVPRLDEK L+ +  FI  +  +
Sbjct: 181 ESYKNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHL 217

BLAST of Lag0004945 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 255.0 bits (650), Expect = 5.5e-68
Identity = 123/223 (55.16%), Postives = 164/223 (73.54%), Query Frame = 0

Query: 1   MASEGKIQTK-PKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVE 60
           MA E  ++ K P+ILCLHGFRTSG IL+  I KWP ++L   D  FLDAPFP++G S+VE
Sbjct: 19  MAPEIHLKPKNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAPFPATGKSDVE 78

Query: 61  GIFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQ 120
             FDPPY+EW++ ++    Y N +ECL +I++YM+++GPFDGLLGFSQGA L+AA+PG+Q
Sbjct: 79  RFFDPPYYEWYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQ 138

Query: 121 EKGIALTKVPKIKFVIIISGSK-----FRSPSVAAEKAYSSSIKCPSLHFLSEEDFLMPS 180
           E+G ALTKVPK+KF++IISG+K     F  P  AA  A+SS ++CPSLHF+ E DFL   
Sbjct: 139 EQGSALTKVPKVKFLVIISGAKIPGLMFGEPK-AAVNAFSSPVRCPSLHFIGERDFLKIE 198

Query: 181 GLKLLESFFEPLVINHPKGHTVPRLDEKGLKVMENFIQRISKM 218
           G  L+ESF EP+VI+H  GH +P+LD K  + M +F Q I +M
Sbjct: 199 GEVLVESFVEPVVIHHSGGHIIPKLDTKAEETMLSFFQSIRQM 240

BLAST of Lag0004945 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 216.5 bits (550), Expect = 2.2e-56
Identity = 102/166 (61.45%), Postives = 130/166 (78.31%), Query Frame = 0

Query: 1   MASEGK-IQTKPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPFPSSGTSEVE 60
           M SEG+ I  KP+ LCLHGFRTSG I+K Q+ KWP SV+++ D  FLDAPFP  G S+VE
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 61  GIFDPPYFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLLGFSQGAALSAALPGLQ 120
           GIFDPPY+EWF+F+++   Y N ++CL+++++ M++ GPFDGL+GFSQGA LS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 121 EKGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLHFL 166
            KGIA  KVPKIKFVIII G+K +S  + AE AYSSS++  SLHFL
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKL-AENAYSSSLETLSLHFL 165

BLAST of Lag0004945 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 79.0 bits (193), Expect = 5.4e-15
Identity = 65/221 (29.41%), Postives = 95/221 (42.99%), Query Frame = 0

Query: 10  KPKILCLHGFRTSGAILKKQIEKWPTSVLNQFDFHFLDAPF----------PSSGTSE-- 69
           K +ILCLHGFR + +  K +       + N  +  F+DAP           P SG     
Sbjct: 353 KLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCNKK 412

Query: 70  ----VEGIFDPP----------YFEWFRFSEDTNSYVNLKECLQFIDNYMLEHGPFDGLL 129
               V   FD P           F+  ++   T  +    + L ++     E GPFDG+L
Sbjct: 413 FAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGF---DKSLTYLKTAFEEKGPFDGIL 472

Query: 130 GFSQGAALSAALPGLQEKGIALTKVPKIKFVIIISGSKFRSPSVAAEKAYSSSIKCPSLH 189
           GFSQGAA++AA+ G QE+   L      +F ++ SG    +P    E     SIKCPSLH
Sbjct: 473 GFSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSGF---TPWPLLEMKEKRSIKCPSLH 532

Query: 190 FL----SEEDFLMPSGLKLLESFFE---PLVINHPKGHTVP 198
                  ++  ++      L   FE     ++ H  GH +P
Sbjct: 533 IFGSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564