Lag0004932 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004932
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionpurine permease 1
Locationchr6: 8665282 .. 8669455 (+)
RNA-Seq ExpressionLag0004932
SyntenyLag0004932
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAGCCACCATAAAAGGGCCCTCCTCGCTTTCAACGTCGTCTTGTTATCCATCGGCAACTGCGGCGGCCCTCTGATTATGCACCTCTACTTCATCCACGGCGGCAACCACGTATGGCTCTCCAGTTGGCTTTTAACCGGTGGCTGGCCCATCATCCTCCTCCCCTTGGCCATCAGCTACTTCCATCGCCGCCGCACCGCCCCTACCGCTTCCACGAAGTTGGTCTTCATGAAGTCGCGTTTGTTTTTGGCCTCCGTCGTAATCGGCGTTCTCACCGAACTCGACGACTACCTGTACGCTTACGGCATGGCGCGGCTGTCGTTGTCGACTTCTTCGCTGATTGTTGCGTGCCAGTTGGCGTTCACGACAGTGTTCGCGTTTCTGCTGGTGAAGCAAAAGTTCACGAGCTACCCTATAAACGCCGTCGTTTTACTGACGGTTGGAGGGGCGGTTTTGGCACTCCACACCAGCGGAGACCGACCGGCTGGAGAATCGAATAAAGAGTACATCGCCGGATTTCTGATGATGGTGGCGGCGGCGGTTCTGTACGGATTTGTGTTGCCGTTAATCGAATTGACGAAGGCGAAGCAGAGAATTACATATACGTTGGTATTGGAAATTCAGTTGGTGATATCTTTATTTTCCACCATTCTCTGCACCATTGGAATGTTAATCAACAATGATTTCCAGGTCTATCTCTCTCCCTCTAACTTCCCCATCACTACTTTTTCAACCATATCCACCATAAAATAAAATATAAAATGTTTAAAATATCATTTTGAAAATTTTCTAAATTTACCCTTTTTTGATACCAGGAAAACTGAAGGAAATAATTTTAAATCTAGTGATTTGAAATTCACATTTTATTTTTAGGCCCCGTTTAATTCGTGACTTGAAAATTGTTTTCGAAAATAAAGAATTAATTTATTATTTCCATTATATCTAAAAACATGTTTGATTCCAATTTAAAAACTTTAAATTTGAATTAAAAATTTGAAAACTATGTTTGGTTATGAATTTGAAAATGCATGAAAATATTAATATGTGCTTGGTAAAATTTTTTTTTTTAGAAAAAGTTAATTTAATATGATTATTTTTCTACTTGACTTTTATTCATTTTTAGAGAGATTATAAATCAATGATGTCCTTTCAAAAAAAAAAAAAAAAATCAATGATGCTGGAATTGTTTCTTCAAAAAAAAAAAAAAATTGTTGGAATTGAAATTATAAGAAAAAAAAAAGATATAACATATAGTTGTATTATATGAATCTTTTGATTTGTAAAGTGGATGAGTATTGTTCACACGTAACTTCGGGGATGGAGACACGTGGAATAAATAATATATTTGTATTAATGTAATATGCGTGTGGGTGTACAATCTCTAACTTTAATCCTCTGATTCTCTCTCTTGTGTGTGAATGTGTTTCACTCAAGGGTTGGAACGCTTGATCCCGAGTCTGAAGCTTCAATGGATTCTTGGAGCTTGAAGTCTTCTAGTCTTGAAGGAGTCTTCAGTCTTCAATAAGTGTTGATGTCCTGAAGAGTCTTTGATCTTCAAGGAGTGTTGAAGTCTTGAAGAGTATTTGGTCTTCAAGAAATGTTGATGTCCTGAAGAGTTTTTGATCTTCGAGGAGTGTTGAGGTCTTGAAGAGTGTTTGGTCTTCAAGAAATGTTGTTGTCCTGAAGTGCTTTTTGATCTTCAAGGAGTGTTGAGGTCTTGAAGAGCGTTTGATCTTCAAGAAGTGTTAATGTCCTGAAGAGTATTTGATCTTCAAGGAGTGTTGAAGTCTTGAAGGGTGTTTGGTCTTCAAAGAGTGTTGAAGTCTTGAAGAGTGTTTGGTCTTCAAGAAGTGTTCAACACTTAGACTTGTGGATTGCAGAGAGTCTTCAGTCTTCAGACTTGGTGGAGTCTCTCTGAATTCTCTAGTTCTGCTTGAGAGCTTTGAAACTCTGAACTCTTGTCTTTGTGAGAGTGTAGAATTTCTGAACCCCTGAAATGGAGGAGAACCCCTCTATTTATAGAGTTCTCGCTGGATGGACCCTGGTGACTTTTGGGTCTAACAACTTGGGCTTGGGCTTTTATTGGGCTTGGGTCTGGTTTATATTTGACCCAAAACCATATGACCCAAATATTTAAGAGACCCAAATTTGATCCAATTAATTATTTTCTTTAATTAATTGGTCAAATCAATTAATCTGATCAAAATAATTTATTTTTAATTAATTGATCAAATGGACATTTGACCCTAAAAATTTATAATTGGCCAACTTTGTCGTCAAATTAAATTTGGGACACATGTCGATTTATAATTGGTCCCAAATTTAATTAATTATAATTTTGTCATTAATTTTAAATAATGACGTGGCAATTTGTCATTGGCCAAAATTTCTCCTTCAACAAGTATATCAAAATAGGTAGAATTATGTAAAAAGTAAAAATAACAACCAAAACATCAGTATCAATCAAACACACTATTTATACAATAGTAATTTTGTATTTTTTTTTCCTTAGTCGAAGAGAATTTGGATATCAAACATAAAAAGATGTATCCTGTGATTTTAAAGTTGTACTTAGTGTTTCCATTATTTTGAAAACAAAATTGGAAAACAATAAAATTAAAACAACATTTTATCGCTTTTGAAAACATACCATTTTTTTAGTTGTTTTCGAAAATTGCTTTTGAAAATAGGTATACCAAACACGTTTTCATGGTTTCCCTTGTATTTTTTATTTTCGAAAACATAAAAACGAAAACGGATCATGAACCAAATGGGGCCTTAAATTCTTTGTCTTGTTCAGAAGGAAAGATTTAAAATAAATATATTTAAAATTTTGTGTTTGAAATGGAAATTAATTGGATGAAGACTTTAAAAGAATTTACTAATATAGACTTTTAATTTGATTTATTAATAAAAAATTAAATATAATAAAAATAAATAAGTAATTTAACTCTCATTTCGATGAAATCCTGAAAAATGGATAGTTTTAGTTGGTAAAATTTGTCTTTTTAAAATCAGTTTTCTTTTAAAATCGAAAATCAAACCAAACCAAATTGGATTTTTTTGTTCGGTTCCAACCGAAATTGCGGCTCGGTTTGGTTTCAACTTTTTTTTTTTTGAATTTTCAATTCAATTTTGATTTAGTCCTCCAACGAAATCGAATCGTAAACACCTTTGAAATGTTAGTACTAAAAATATGTTAGACCATATAATTTCAAAATTCATATATCATCTCTTTTTCAACGTCTAGTCTAAGCACTTCGTTAAAAACATGAATTATTATCTCATATCTGGACGGTATGAATTTTTGTTCACCTAAATTTGGTCGTCCAATAGCAAGTTGGCATGATATGTTTCTTTACCAAAACACTAGAGAAAGAAAATATTTCAAAAAAAAAAAAAAACTAGAGAAAGAAAATAATCTCATACACATGATTAGCCAACACATACCTTACTAGTGTCAACTTGTTATTGGGCCACATCTCCAGCTATAGTAATACCATTCATAAGGACAAATTATTCAATGAGACTGTCACATTGTAGTTGTCTAACTATTTTTATGTAGATCTTAATATTTTTTTTTTGTCTCTTAATAACCGTTGAATAAAAATTGAGGTACTTTTACAAATTCTCTTCTTACATCCAACGAACGATGTAAAACTGCCAACCTTATTGTGACGACTCGACTCTCAATCAAATAATGCATGAGGTAGGTCTTAATTTCTCAATTAAAAAGAGAATGATAATATGATGAATGAAAAAAAATTCCTACTAGAGGATTTTCATACTTTCTTTGTTTACATTATAATTTTATATTTATAAATATTTCTTTATTGCTAATCCACCTCACATCAAATGAGAAAGTAAGTATCAGTCAGAAAATTTAGTTTGATAATTGGAACAGGTGATTCCAAGAGAGGGCAGAGAATTTGGACTTGGAGAGACTAAGTACTACGTGATATTAGTGTGCAGTCCCATAATCTGGCAAGCTTTCTTCGTCGGAGCCGTCGGAGTTATCTTCTACTCTTCGTCGCTGTTTTCAGGGATAGTCATTGCGCTGCTATTGCCGGCGACGGAAATTATCGCTGTCATTTTTTTTCGGGAAAATTTTCAGGCAGAAAAGGGAGTTTCTCTCGCTCTCAACCTTTGGAGTTTTATCTCTTATTTCTAA

mRNA sequence

ATGGAAAGCCACCATAAAAGGGCCCTCCTCGCTTTCAACGTCGTCTTGTTATCCATCGGCAACTGCGGCGGCCCTCTGATTATGCACCTCTACTTCATCCACGGCGGCAACCACGTATGGCTCTCCAGTTGGCTTTTAACCGGTGGCTGGCCCATCATCCTCCTCCCCTTGGCCATCAGCTACTTCCATCGCCGCCGCACCGCCCCTACCGCTTCCACGAAGTTGGTCTTCATGAAGTCGCGTTTGTTTTTGGCCTCCGTCGTAATCGGCGTTCTCACCGAACTCGACGACTACCTGTACGCTTACGGCATGGCGCGGCTGTCGTTGTCGACTTCTTCGCTGATTGTTGCGTGCCAGTTGGCGTTCACGACAGTGTTCGCGTTTCTGCTGGTGAAGCAAAAGTTCACGAGCTACCCTATAAACGCCGTCGTTTTACTGACGGTTGGAGGGGCGGTTTTGGCACTCCACACCAGCGGAGACCGACCGGCTGGAGAATCGAATAAAGAGTACATCGCCGGATTTCTGATGATGGTGGCGGCGGCGGTTCTGTACGGATTTGTGTTGCCGTTAATCGAATTGACGAAGGCGAAGCAGAGAATTACATATACGTTGGTATTGGAAATTCAGTTGGTGATATCTTTATTTTCCACCATTCTCTGCACCATTGGAATGTTAATCAACAATGATTTCCAGGTGATTCCAAGAGAGGGCAGAGAATTTGGACTTGGAGAGACTAAGTACTACGTGATATTAGTGTGCAGTCCCATAATCTGGCAAGCTTTCTTCGTCGGAGCCGTCGGAGTTATCTTCTACTCTTCGTCGCTGTTTTCAGGGATAGTCATTGCGCTGCTATTGCCGGCGACGGAAATTATCGCTGTCATTTTTTTTCGGGAAAATTTTCAGGCAGAAAAGGGAGTTTCTCTCGCTCTCAACCTTTGGAGTTTTATCTCTTATTTCTAA

Coding sequence (CDS)

ATGGAAAGCCACCATAAAAGGGCCCTCCTCGCTTTCAACGTCGTCTTGTTATCCATCGGCAACTGCGGCGGCCCTCTGATTATGCACCTCTACTTCATCCACGGCGGCAACCACGTATGGCTCTCCAGTTGGCTTTTAACCGGTGGCTGGCCCATCATCCTCCTCCCCTTGGCCATCAGCTACTTCCATCGCCGCCGCACCGCCCCTACCGCTTCCACGAAGTTGGTCTTCATGAAGTCGCGTTTGTTTTTGGCCTCCGTCGTAATCGGCGTTCTCACCGAACTCGACGACTACCTGTACGCTTACGGCATGGCGCGGCTGTCGTTGTCGACTTCTTCGCTGATTGTTGCGTGCCAGTTGGCGTTCACGACAGTGTTCGCGTTTCTGCTGGTGAAGCAAAAGTTCACGAGCTACCCTATAAACGCCGTCGTTTTACTGACGGTTGGAGGGGCGGTTTTGGCACTCCACACCAGCGGAGACCGACCGGCTGGAGAATCGAATAAAGAGTACATCGCCGGATTTCTGATGATGGTGGCGGCGGCGGTTCTGTACGGATTTGTGTTGCCGTTAATCGAATTGACGAAGGCGAAGCAGAGAATTACATATACGTTGGTATTGGAAATTCAGTTGGTGATATCTTTATTTTCCACCATTCTCTGCACCATTGGAATGTTAATCAACAATGATTTCCAGGTGATTCCAAGAGAGGGCAGAGAATTTGGACTTGGAGAGACTAAGTACTACGTGATATTAGTGTGCAGTCCCATAATCTGGCAAGCTTTCTTCGTCGGAGCCGTCGGAGTTATCTTCTACTCTTCGTCGCTGTTTTCAGGGATAGTCATTGCGCTGCTATTGCCGGCGACGGAAATTATCGCTGTCATTTTTTTTCGGGAAAATTTTCAGGCAGAAAAGGGAGTTTCTCTCGCTCTCAACCTTTGGAGTTTTATCTCTTATTTCTAA

Protein sequence

MESHHKRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRRRTAPTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTTVFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLYGFVLPLIELTKAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGLGETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQAEKGVSLALNLWSFISYF
Homology
BLAST of Lag0004932 vs. NCBI nr
Match: XP_022131268.1 (purine permease 3-like [Momordica charantia])

HSP 1 Score: 494.2 bits (1271), Expect = 8.5e-136
Identity = 251/316 (79.43%), Postives = 281/316 (88.92%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           KRALL FN VLL+IGNCGGPL+M LYF+HGG  VWLSSWL TGGWPII +PLAISYFHRR
Sbjct: 12  KRALLIFNCVLLAIGNCGGPLVMRLYFVHGGKRVWLSSWLETGGWPIIFIPLAISYFHRR 71

Query: 66  RTAPTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTTV 125
           R A   ST L+FMK RLFLA+ VIGV+T  DDYLYAYG+ARL +STS+LI+ACQLAFT V
Sbjct: 72  RAAAGESTTLIFMKPRLFLAATVIGVITGFDDYLYAYGVARLPVSTSALIIACQLAFTAV 131

Query: 126 FAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLYG 185
           FA+LLVKQKFTSY INAVVL+T+GGAVLALHTSGDRPAGES K Y+AGFLM VAAA+LYG
Sbjct: 132 FAYLLVKQKFTSYSINAVVLMTIGGAVLALHTSGDRPAGESTKAYVAGFLMTVAAALLYG 191

Query: 186 FVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGLG 245
           FVLPL+ELT  KAKQ+ITYTLVLEIQLV+SLF+T++CT+GMLINNDFQVIPRE REFGLG
Sbjct: 192 FVLPLVELTYKKAKQQITYTLVLEIQLVMSLFATLVCTVGMLINNDFQVIPREAREFGLG 251

Query: 246 ETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQAE 305
           ETKYYV+L  S IIWQ FF+GA+GVIF SSSLFSGIVIA+LLP TEI+AV+ F E FQAE
Sbjct: 252 ETKYYVVLAFSAIIWQGFFLGAIGVIFCSSSLFSGIVIAVLLPVTEILAVVIFSEKFQAE 311

Query: 306 KGVSLALNLWSFISYF 320
           KGVSLALNLW F+SYF
Sbjct: 312 KGVSLALNLWGFVSYF 327

BLAST of Lag0004932 vs. NCBI nr
Match: XP_022961838.1 (purine permease 3-like [Cucurbita moschata])

HSP 1 Score: 493.4 bits (1269), Expect = 1.4e-135
Identity = 257/321 (80.06%), Postives = 284/321 (88.47%), Query Frame = 0

Query: 2   ESHHKRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISY 61
           E+  K+ LLAFN VL+SIGNCGGPLI+ LYFIHGGN VWLSSWLLTGGWPIILLPL ISY
Sbjct: 43  EATVKKFLLAFNCVLMSIGNCGGPLILRLYFIHGGNRVWLSSWLLTGGWPIILLPLVISY 102

Query: 62  FHRRRT-APTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQL 121
           F RRRT A   +T+ +F+K RLF AS VIG+LT LD+YLYAYG+ARL +STSSLI+A QL
Sbjct: 103 FQRRRTSAAGTTTEFIFIKPRLFFASAVIGLLTGLDNYLYAYGVARLPVSTSSLIIASQL 162

Query: 122 AFTTVFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAA 181
           AFT  FAFLLVKQ+FTSY +NAVVLLT+GGA+LALH+SGDRPAGESNKEY+AGFLM V A
Sbjct: 163 AFTAGFAFLLVKQRFTSYSVNAVVLLTLGGAILALHSSGDRPAGESNKEYVAGFLMTVGA 222

Query: 182 AVLYGFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGR 241
           +VLYGFVLPLIELT  KAKQ +TYTLVLE Q VISLF+TILCTIGMLINNDFQ IPREG 
Sbjct: 223 SVLYGFVLPLIELTYKKAKQNLTYTLVLETQFVISLFATILCTIGMLINNDFQAIPREGT 282

Query: 242 EFGLGETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRE 301
           EFGLG+TKYYVIL  S +IWQ FFVGAVGVIFYSSSLFSG+VIALLLPATEI+AVIFFRE
Sbjct: 283 EFGLGKTKYYVILALSTVIWQCFFVGAVGVIFYSSSLFSGVVIALLLPATEILAVIFFRE 342

Query: 302 NFQAEKGVSLALNLWSFISYF 320
            FQAEKGVSLALNLW F+SYF
Sbjct: 343 KFQAEKGVSLALNLWGFVSYF 363

BLAST of Lag0004932 vs. NCBI nr
Match: KAG6598711.1 (Purine permease 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 492.7 bits (1267), Expect = 2.5e-135
Identity = 257/321 (80.06%), Postives = 284/321 (88.47%), Query Frame = 0

Query: 2   ESHHKRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISY 61
           E+  K+ LLAFN VL+SIGNCGGPLI+ LYFIHGGN VWLSSWLLTGGWPIILLPL ISY
Sbjct: 43  EAAVKKFLLAFNCVLMSIGNCGGPLILRLYFIHGGNRVWLSSWLLTGGWPIILLPLVISY 102

Query: 62  FHRRRT-APTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQL 121
           F RRRT A   +T+ +F+K RLF AS VIG+LT LD+YLYAYG+ARL +STSSLI+A QL
Sbjct: 103 FQRRRTSAAGTTTEFIFIKPRLFFASAVIGLLTGLDNYLYAYGVARLPVSTSSLIIASQL 162

Query: 122 AFTTVFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAA 181
           AFT  FAFLLVKQ+FTSY +NAVVLLT+GGA+LALH+SGDRPAGESN+EYIAGFLM V A
Sbjct: 163 AFTAGFAFLLVKQRFTSYSVNAVVLLTLGGAILALHSSGDRPAGESNREYIAGFLMTVGA 222

Query: 182 AVLYGFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGR 241
           +VLYGFVLPLIELT  KAKQ +TYTLVLE Q VISLF+TILCTIGMLINNDFQ IPREG 
Sbjct: 223 SVLYGFVLPLIELTYKKAKQNLTYTLVLETQFVISLFATILCTIGMLINNDFQAIPREGT 282

Query: 242 EFGLGETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRE 301
           EFGLG+TKYYVIL  S +IWQ FFVGAVGVIFYSSSLFSG+VIALLLPATEI+AVIFFRE
Sbjct: 283 EFGLGKTKYYVILALSTVIWQCFFVGAVGVIFYSSSLFSGVVIALLLPATEILAVIFFRE 342

Query: 302 NFQAEKGVSLALNLWSFISYF 320
            FQAEKGVSLALNLW F+SYF
Sbjct: 343 KFQAEKGVSLALNLWGFVSYF 363

BLAST of Lag0004932 vs. NCBI nr
Match: KAG7029652.1 (Purine permease 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 492.7 bits (1267), Expect = 2.5e-135
Identity = 257/321 (80.06%), Postives = 284/321 (88.47%), Query Frame = 0

Query: 2   ESHHKRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISY 61
           E+  K+ LLAFN VL+SIGNCGGPLI+ LYFIHGGN VWLSSWLLTGGWPIILLPL ISY
Sbjct: 422 EAAVKKFLLAFNCVLMSIGNCGGPLILRLYFIHGGNRVWLSSWLLTGGWPIILLPLVISY 481

Query: 62  FHRRRT-APTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQL 121
           F RRRT A   +T+ +F+K RLF AS VIG+LT LD+YLYAYG+ARL +STSSLI+A QL
Sbjct: 482 FQRRRTSAAGTTTEFIFIKPRLFFASAVIGLLTGLDNYLYAYGVARLPVSTSSLIIASQL 541

Query: 122 AFTTVFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAA 181
           AFT  FAFLLVKQ+FTSY +NAVVLLT+GGA+LALH+SGDRPAGESN+EYIAGFLM V A
Sbjct: 542 AFTAGFAFLLVKQRFTSYSVNAVVLLTLGGAILALHSSGDRPAGESNREYIAGFLMTVGA 601

Query: 182 AVLYGFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGR 241
           +VLYGFVLPLIELT  KAKQ +TYTLVLE Q VISLF+TILCTIGMLINNDFQ IPREG 
Sbjct: 602 SVLYGFVLPLIELTYKKAKQNLTYTLVLETQFVISLFATILCTIGMLINNDFQAIPREGT 661

Query: 242 EFGLGETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRE 301
           EFGLG+TKYYVIL  S +IWQ FFVGAVGVIFYSSSLFSG+VIALLLPATEI+AVIFFRE
Sbjct: 662 EFGLGKTKYYVILALSTVIWQCFFVGAVGVIFYSSSLFSGVVIALLLPATEILAVIFFRE 721

Query: 302 NFQAEKGVSLALNLWSFISYF 320
            FQAEKGVSLALNLW F+SYF
Sbjct: 722 KFQAEKGVSLALNLWGFVSYF 742

BLAST of Lag0004932 vs. NCBI nr
Match: XP_022997185.1 (purine permease 3-like [Cucurbita maxima])

HSP 1 Score: 491.9 bits (1265), Expect = 4.2e-135
Identity = 256/317 (80.76%), Postives = 282/317 (88.96%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           K+ LLAFN VL+SIGNCGGPLI+ LYFIHGGN VWLSSWLLTGGWPIILLPL ISYF R 
Sbjct: 28  KKFLLAFNCVLMSIGNCGGPLILRLYFIHGGNRVWLSSWLLTGGWPIILLPLVISYFQRL 87

Query: 66  RT-APTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTT 125
           RT A   +T+L+F+K RLF AS VIG+LT LD+YLYAYG+ARL +STSSLI+A QLAFT 
Sbjct: 88  RTSAAGTTTELIFIKPRLFFASAVIGLLTGLDNYLYAYGVARLPVSTSSLIIASQLAFTA 147

Query: 126 VFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLY 185
            FAFLLVKQ+FTSY +NAVVLLT+GGA+LALH+SGDRPAGESNKEYIAGFLM V A+VLY
Sbjct: 148 GFAFLLVKQRFTSYSVNAVVLLTLGGAILALHSSGDRPAGESNKEYIAGFLMTVGASVLY 207

Query: 186 GFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGL 245
           GFVLPLIELT  KAKQ +TYTLVLE Q VISLF+TILCTIGMLINNDFQ IPREG EFGL
Sbjct: 208 GFVLPLIELTYKKAKQNLTYTLVLETQFVISLFATILCTIGMLINNDFQAIPREGTEFGL 267

Query: 246 GETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQA 305
           G+T YYVIL  S ++WQ FFVGAVGVIFYSSSLFSG+VIALLLPATEI+AVIFFRENFQA
Sbjct: 268 GKTNYYVILALSTVVWQCFFVGAVGVIFYSSSLFSGVVIALLLPATEILAVIFFRENFQA 327

Query: 306 EKGVSLALNLWSFISYF 320
           EKGVSLALNLW F+SYF
Sbjct: 328 EKGVSLALNLWGFVSYF 344

BLAST of Lag0004932 vs. ExPASy Swiss-Prot
Match: Q9FZ96 (Purine permease 1 OS=Arabidopsis thaliana OX=3702 GN=PUP1 PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 7.3e-98
Identity = 187/324 (57.72%), Postives = 243/324 (75.00%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           K  L+  N ++L+IG CGGPL+  LYF +GG  +W  S+L T G+PIIL+PL +S+  RR
Sbjct: 2   KNGLIIINCIILTIGTCGGPLLTRLYFTNGGKRIWFMSFLSTAGFPIILIPLLVSFLSRR 61

Query: 66  RTAPTAS-------TKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVAC 125
           R+    +       TKL  M++ LF+AS+VIG+LT LD+YLY+YG+A L +STSSLI+  
Sbjct: 62  RSNRNPNNAENKRKTKLFLMETPLFIASIVIGLLTGLDNYLYSYGLAYLPVSTSSLIIGT 121

Query: 126 QLAFTTVFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMV 185
           QLAF  +FAFLLVKQKFT + INAVVLLTVG  +LALH+ GD+PA ES KEY+ GFLM V
Sbjct: 122 QLAFNALFAFLLVKQKFTPFSINAVVLLTVGIGILALHSDGDKPAKESKKEYVVGFLMTV 181

Query: 186 AAAVLYGFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPRE 245
            AA+LY F+LPL+ELT  KA+Q IT+ LVLEIQ+V+ L +T  C IGM I  DF+VI RE
Sbjct: 182 VAALLYAFILPLVELTYKKARQEITFPLVLEIQMVMCLAATFFCVIGMFIVGDFKVIARE 241

Query: 246 GREFGL-GETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIF 305
            REF + G   YY ++V + IIWQ FF+GA+G++F +SSL SG++I++LLP TE+ AV+ 
Sbjct: 242 AREFKIGGSVFYYALIVITGIIWQGFFLGAIGIVFCASSLASGVLISVLLPVTEVFAVVC 301

Query: 306 FRENFQAEKGVSLALNLWSFISYF 320
           FRE FQAEKGVSL L+LW F+SYF
Sbjct: 302 FREKFQAEKGVSLLLSLWGFVSYF 325

BLAST of Lag0004932 vs. ExPASy Swiss-Prot
Match: Q9FZ95 (Purine permease 3 OS=Arabidopsis thaliana OX=3702 GN=PUP3 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 4.7e-97
Identity = 179/316 (56.65%), Postives = 243/316 (76.90%), Query Frame = 0

Query: 7   RALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRRR 66
           +AL+  N ++L+IGNCGGPLIM LYF +GG  +W S++L T G+P+I +PL  SY  RRR
Sbjct: 3   KALVIINCIILAIGNCGGPLIMRLYFNNGGKRIWFSTFLETAGFPVIFIPLLFSYITRRR 62

Query: 67  TAPTA-STKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTTV 126
           +     ST    +K RL +A+V++G+L+  D+YLYAYG+A L +ST++LI+A QLAF  +
Sbjct: 63  SNNVGDSTSFFLIKPRLLIAAVIVGILSGFDNYLYAYGIAYLPVSTAALIIASQLAFIAI 122

Query: 127 FAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLYG 186
           F+F +VK KFT + INAVVLLTVG AVL +HT  D+P  E++K+YI GFL+ VAAAV+Y 
Sbjct: 123 FSFFMVKHKFTPFTINAVVLLTVGAAVLGMHTETDKPVHETHKQYITGFLITVAAAVMYA 182

Query: 187 FVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGLG 246
           F+LPL+EL   KAKQ ++YTLVLE QL++ L ++I+  IGM I  DF+ +P+E REF LG
Sbjct: 183 FILPLVELAYQKAKQTMSYTLVLEFQLILCLLASIVSVIGMFIAGDFKALPKEAREFKLG 242

Query: 247 ETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQAE 306
           E  +YV+ V S IIWQ FF+GA+G+IF +SSL SGI+I++LLP TE++AVIF+ E FQAE
Sbjct: 243 EALFYVVAVFSAIIWQGFFLGAIGLIFSTSSLVSGIMISVLLPITEVLAVIFYHEKFQAE 302

Query: 307 KGVSLALNLWSFISYF 320
           KG+SLAL+LW F+SYF
Sbjct: 303 KGLSLALSLWGFVSYF 318

BLAST of Lag0004932 vs. ExPASy Swiss-Prot
Match: Q94GB1 (Purine permease 2 OS=Arabidopsis thaliana OX=3702 GN=PUP2 PE=1 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 6.8e-88
Identity = 172/328 (52.44%), Postives = 234/328 (71.34%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           K  L+  N + L+IGNCGGPL+M LYF +GG  +W  S+L T G P+I  PL +S+  RR
Sbjct: 4   KTVLVIINCIFLAIGNCGGPLMMRLYFQNGGERIWFPSFLQTVGCPLIFFPLLLSFLRRR 63

Query: 66  RTAPTASTKLVF-MKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTT 125
           R      T   F MK  LF+A++V+G+L   D+YLY+YG+A + +ST+SLI++ QL FT 
Sbjct: 64  RCLEEQETTPFFLMKPPLFIAAIVVGLLVGFDNYLYSYGLAYIPVSTASLIISAQLGFTA 123

Query: 126 VFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLY 185
           +FAF +VKQKFT + INA+VLLT G  VLAL++  D+ A E++KEY+ GF+M + AA+LY
Sbjct: 124 LFAFFMVKQKFTPFTINAIVLLTGGAVVLALNSDSDKLANETHKEYVVGFIMTLGAALLY 183

Query: 186 GFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDF-----------Q 245
           GF+LPL+EL+  K+ QRITYTL LE Q+V+   +T +C +GML   DF           +
Sbjct: 184 GFILPLVELSYKKSGQRITYTLALEFQMVLCFAATCVCLVGMLAAGDFKVKHALFIFKNR 243

Query: 246 VIPREGREFGLGETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEII 305
           VI  E R+F LGE+ YYV++V + IIWQAFFVGA+G+IF +SSL SGI+++ LLP T I+
Sbjct: 244 VIAGEARDFKLGESLYYVVIVFTAIIWQAFFVGAIGLIFCASSLVSGIMVSALLPVTVIL 303

Query: 306 AVIFFRENFQAEKGVSLALNLWSFISYF 320
           AVI F+E FQA KGV+LAL+LW  +SYF
Sbjct: 304 AVICFQEKFQAGKGVALALSLWGSVSYF 331

BLAST of Lag0004932 vs. ExPASy Swiss-Prot
Match: Q9ZUH3 (Probable purine permease 5 OS=Arabidopsis thaliana OX=3702 GN=PUP5 PE=3 SV=2)

HSP 1 Score: 182.6 bits (462), Expect = 7.1e-45
Identity = 112/312 (35.90%), Postives = 176/312 (56.41%), Query Frame = 0

Query: 9   LLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRRRTA 68
           LL F+   + I      L+  LYF +GG   W+ SW+   GWPI  L L  +Y  ++   
Sbjct: 39  LLFFSGAAMLIAFPASSLLSRLYFSNGGKSKWIISWVAVAGWPITCLILLPTYIFQKIKP 98

Query: 69  PTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTTVFAF 128
              +TKLV       L+ VV+G L+  D+ +YAY  A L  STSSL+ +  LAF+ +F +
Sbjct: 99  TPLNTKLV-------LSYVVLGFLSAADNLMYAYAYAYLPASTSSLLASSSLAFSALFGY 158

Query: 129 LLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLYGFVL 188
           L+VK    +  IN++V++T   A++AL +S DR +  SN +Y AGF   +  + L+G + 
Sbjct: 159 LIVKNPLNASVINSIVVITGAMAIIALDSSSDRYSYISNSQYFAGFFWDIMGSALHGLIF 218

Query: 189 PLIEL--TKAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGLGETK 248
            L EL   K   R ++ + LE Q+++SL +    TIGM+++NDFQ +  E + F  GE+ 
Sbjct: 219 ALSELLFVKLLGRRSFHVALEQQVMVSLTAFAFTTIGMVVSNDFQGMSHEAKSFKGGESL 278

Query: 249 YYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQAEKGV 308
           Y  +LV S + +Q   +GA  V+F +S++ +G++ A+ +P T + AVI   +     K +
Sbjct: 279 YTQVLVWSAVTFQLGVLGATAVLFLASTVMAGVLNAVRVPITSVAAVILMHDPMSGFKIL 338

Query: 309 SLALNLWSFISY 319
           SL L  W F SY
Sbjct: 339 SLVLTFWGFSSY 343

BLAST of Lag0004932 vs. ExPASy Swiss-Prot
Match: Q9LPF6 (Probable purine permease 11 OS=Arabidopsis thaliana OX=3702 GN=PUP11 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.3e-43
Identity = 105/312 (33.65%), Postives = 179/312 (57.37%), Query Frame = 0

Query: 9   LLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRRRTA 68
           L++ N+  L  G     L+   Y+  GGN  W+++ + T  +PI+ +PL +        +
Sbjct: 45  LVSVNIFFLIGGQAASVLLGRFYYDEGGNSKWMATLVQTAAFPILYIPLLLLPSSASVES 104

Query: 69  PTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTTVFAF 128
             +S  L +    + L  V++GV+   D+ LY+ G+  LS ST SLI A QLAF  VF++
Sbjct: 105 SESSCSLKY----IVLIYVLLGVIIAGDNMLYSVGLLYLSASTYSLICATQLAFNAVFSY 164

Query: 129 LLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLYGFVL 188
            +  QKFT+  +N+VVLL+   A++AL+   D P+G S  +YI GF+  +AA+ LY  +L
Sbjct: 165 FINAQKFTALILNSVVLLSFSAALIALNDDADTPSGVSRSKYIVGFVCTLAASALYSLLL 224

Query: 189 PLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGLGETK 248
            L++ +  K  +R T+++VLE+Q+  SL +T +  IG+  + +++ +  E   +  G+  
Sbjct: 225 SLMQFSFEKILKRETFSVVLEMQIYTSLVATCVSVIGLFASGEWRTLHGEMEGYHKGQAS 284

Query: 249 YYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQAEKGV 308
           Y + LV + + WQ   VG VG+IF  +SLFS ++  L L  T + A++ FR+     K +
Sbjct: 285 YVLTLVWTAVTWQVCSVGVVGLIFLVTSLFSNVISTLSLAVTPLAALVVFRDKMSGVKIM 344

Query: 309 SLALNLWSFISY 319
           ++ + +W F SY
Sbjct: 345 AMLIAIWGFASY 352

BLAST of Lag0004932 vs. ExPASy TrEMBL
Match: A0A6J1BSV4 (Probable purine permease OS=Momordica charantia OX=3673 GN=LOC111004539 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 4.1e-136
Identity = 251/316 (79.43%), Postives = 281/316 (88.92%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           KRALL FN VLL+IGNCGGPL+M LYF+HGG  VWLSSWL TGGWPII +PLAISYFHRR
Sbjct: 12  KRALLIFNCVLLAIGNCGGPLVMRLYFVHGGKRVWLSSWLETGGWPIIFIPLAISYFHRR 71

Query: 66  RTAPTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTTV 125
           R A   ST L+FMK RLFLA+ VIGV+T  DDYLYAYG+ARL +STS+LI+ACQLAFT V
Sbjct: 72  RAAAGESTTLIFMKPRLFLAATVIGVITGFDDYLYAYGVARLPVSTSALIIACQLAFTAV 131

Query: 126 FAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLYG 185
           FA+LLVKQKFTSY INAVVL+T+GGAVLALHTSGDRPAGES K Y+AGFLM VAAA+LYG
Sbjct: 132 FAYLLVKQKFTSYSINAVVLMTIGGAVLALHTSGDRPAGESTKAYVAGFLMTVAAALLYG 191

Query: 186 FVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGLG 245
           FVLPL+ELT  KAKQ+ITYTLVLEIQLV+SLF+T++CT+GMLINNDFQVIPRE REFGLG
Sbjct: 192 FVLPLVELTYKKAKQQITYTLVLEIQLVMSLFATLVCTVGMLINNDFQVIPREAREFGLG 251

Query: 246 ETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQAE 305
           ETKYYV+L  S IIWQ FF+GA+GVIF SSSLFSGIVIA+LLP TEI+AV+ F E FQAE
Sbjct: 252 ETKYYVVLAFSAIIWQGFFLGAIGVIFCSSSLFSGIVIAVLLPVTEILAVVIFSEKFQAE 311

Query: 306 KGVSLALNLWSFISYF 320
           KGVSLALNLW F+SYF
Sbjct: 312 KGVSLALNLWGFVSYF 327

BLAST of Lag0004932 vs. ExPASy TrEMBL
Match: A0A6J1HCZ4 (Probable purine permease OS=Cucurbita moschata OX=3662 GN=LOC111462483 PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 7.0e-136
Identity = 257/321 (80.06%), Postives = 284/321 (88.47%), Query Frame = 0

Query: 2   ESHHKRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISY 61
           E+  K+ LLAFN VL+SIGNCGGPLI+ LYFIHGGN VWLSSWLLTGGWPIILLPL ISY
Sbjct: 43  EATVKKFLLAFNCVLMSIGNCGGPLILRLYFIHGGNRVWLSSWLLTGGWPIILLPLVISY 102

Query: 62  FHRRRT-APTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQL 121
           F RRRT A   +T+ +F+K RLF AS VIG+LT LD+YLYAYG+ARL +STSSLI+A QL
Sbjct: 103 FQRRRTSAAGTTTEFIFIKPRLFFASAVIGLLTGLDNYLYAYGVARLPVSTSSLIIASQL 162

Query: 122 AFTTVFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAA 181
           AFT  FAFLLVKQ+FTSY +NAVVLLT+GGA+LALH+SGDRPAGESNKEY+AGFLM V A
Sbjct: 163 AFTAGFAFLLVKQRFTSYSVNAVVLLTLGGAILALHSSGDRPAGESNKEYVAGFLMTVGA 222

Query: 182 AVLYGFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGR 241
           +VLYGFVLPLIELT  KAKQ +TYTLVLE Q VISLF+TILCTIGMLINNDFQ IPREG 
Sbjct: 223 SVLYGFVLPLIELTYKKAKQNLTYTLVLETQFVISLFATILCTIGMLINNDFQAIPREGT 282

Query: 242 EFGLGETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRE 301
           EFGLG+TKYYVIL  S +IWQ FFVGAVGVIFYSSSLFSG+VIALLLPATEI+AVIFFRE
Sbjct: 283 EFGLGKTKYYVILALSTVIWQCFFVGAVGVIFYSSSLFSGVVIALLLPATEILAVIFFRE 342

Query: 302 NFQAEKGVSLALNLWSFISYF 320
            FQAEKGVSLALNLW F+SYF
Sbjct: 343 KFQAEKGVSLALNLWGFVSYF 363

BLAST of Lag0004932 vs. ExPASy TrEMBL
Match: A0A6J1K8V9 (Probable purine permease OS=Cucurbita maxima OX=3661 GN=LOC111492160 PE=3 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 2.0e-135
Identity = 256/317 (80.76%), Postives = 282/317 (88.96%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           K+ LLAFN VL+SIGNCGGPLI+ LYFIHGGN VWLSSWLLTGGWPIILLPL ISYF R 
Sbjct: 28  KKFLLAFNCVLMSIGNCGGPLILRLYFIHGGNRVWLSSWLLTGGWPIILLPLVISYFQRL 87

Query: 66  RT-APTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTT 125
           RT A   +T+L+F+K RLF AS VIG+LT LD+YLYAYG+ARL +STSSLI+A QLAFT 
Sbjct: 88  RTSAAGTTTELIFIKPRLFFASAVIGLLTGLDNYLYAYGVARLPVSTSSLIIASQLAFTA 147

Query: 126 VFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLY 185
            FAFLLVKQ+FTSY +NAVVLLT+GGA+LALH+SGDRPAGESNKEYIAGFLM V A+VLY
Sbjct: 148 GFAFLLVKQRFTSYSVNAVVLLTLGGAILALHSSGDRPAGESNKEYIAGFLMTVGASVLY 207

Query: 186 GFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGL 245
           GFVLPLIELT  KAKQ +TYTLVLE Q VISLF+TILCTIGMLINNDFQ IPREG EFGL
Sbjct: 208 GFVLPLIELTYKKAKQNLTYTLVLETQFVISLFATILCTIGMLINNDFQAIPREGTEFGL 267

Query: 246 GETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQA 305
           G+T YYVIL  S ++WQ FFVGAVGVIFYSSSLFSG+VIALLLPATEI+AVIFFRENFQA
Sbjct: 268 GKTNYYVILALSTVVWQCFFVGAVGVIFYSSSLFSGVVIALLLPATEILAVIFFRENFQA 327

Query: 306 EKGVSLALNLWSFISYF 320
           EKGVSLALNLW F+SYF
Sbjct: 328 EKGVSLALNLWGFVSYF 344

BLAST of Lag0004932 vs. ExPASy TrEMBL
Match: A0A6J1BS92 (Probable purine permease OS=Momordica charantia OX=3673 GN=LOC111005083 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.2e-133
Identity = 255/316 (80.70%), Postives = 279/316 (88.29%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           K+ LLAFN VLLSIG CG PLIM LYFIHGGN VWL+S LLT  WP+IL PLAISYF RR
Sbjct: 23  KKLLLAFNSVLLSIGTCGSPLIMRLYFIHGGNRVWLTSCLLTAAWPVILFPLAISYFRRR 82

Query: 66  RTAPTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTTV 125
           R+   AS K +FMK RLFLA+ VIG+LT LDDYLYAYG+ARL +STSSLI+ACQL+FT V
Sbjct: 83  RS--RASAKFIFMKPRLFLAAAVIGLLTGLDDYLYAYGVARLPVSTSSLIIACQLSFTAV 142

Query: 126 FAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLYG 185
           FAFLLVKQKFTSY +NAVVLLT+GGAVLALHT+GDRP GESNKEYI GFLM VAAAVLYG
Sbjct: 143 FAFLLVKQKFTSYSVNAVVLLTIGGAVLALHTNGDRPPGESNKEYITGFLMTVAAAVLYG 202

Query: 186 FVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGLG 245
            VLPLIE+   KAKQRITYTLVLEIQLVISLF+T+LCTIGMLINNDFQVIPREGR FGLG
Sbjct: 203 LVLPLIEMAYKKAKQRITYTLVLEIQLVISLFATVLCTIGMLINNDFQVIPREGRVFGLG 262

Query: 246 ETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQAE 305
           E KYYV+LV + +I QAFF+GAVGVI+YSSSLFSGIVIA LLPATEI+AVIFFRE FQAE
Sbjct: 263 EAKYYVVLVATAVISQAFFLGAVGVIYYSSSLFSGIVIAALLPATEILAVIFFREKFQAE 322

Query: 306 KGVSLALNLWSFISYF 320
           KGVSLALNLW F+SYF
Sbjct: 323 KGVSLALNLWGFVSYF 336

BLAST of Lag0004932 vs. ExPASy TrEMBL
Match: A0A1S3BAX3 (Probable purine permease OS=Cucumis melo OX=3656 GN=LOC103488092 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.3e-129
Identity = 244/318 (76.73%), Postives = 276/318 (86.79%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           K+ LLAFN +L+S+GNCGGPLI+ LYFIHGGN VWLSSWL +GGWPIIL+PLAISY HRR
Sbjct: 11  KKVLLAFNSILMSVGNCGGPLILRLYFIHGGNRVWLSSWLFSGGWPIILIPLAISYIHRR 70

Query: 66  RTAPTAS-TKLVFMKSRLFL-ASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFT 125
           RTA   S TKL+FM+  L L  S VIG LT LD+YL+AYGMARL +STSSLI+A QLAFT
Sbjct: 71  RTATAGSKTKLIFMREPLLLFGSAVIGALTGLDNYLFAYGMARLPVSTSSLIIASQLAFT 130

Query: 126 TVFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVL 185
             FA+LLVKQKFTSY +N+VVLLT+GGA+LALH+SGDRPAGESN EYIAGFLM + AAVL
Sbjct: 131 AGFAYLLVKQKFTSYTVNSVVLLTMGGAILALHSSGDRPAGESNGEYIAGFLMTLGAAVL 190

Query: 186 YGFVLPLIEL--TKAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFG 245
           YG +LPLIEL   K KQ +TYTL+LEIQLV+++  T+LCTIGMLINNDFQ IPREGREFG
Sbjct: 191 YGLILPLIELMYKKTKQSLTYTLILEIQLVMAISGTVLCTIGMLINNDFQAIPREGREFG 250

Query: 246 LGETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQ 305
           LG TKYYV+LV S +IWQ FF+GAVGVIFYSSSLFSGIVIALLLPA EI+AVIFFRE FQ
Sbjct: 251 LGNTKYYVVLVMSCVIWQCFFIGAVGVIFYSSSLFSGIVIALLLPAVEILAVIFFREKFQ 310

Query: 306 AEKGVSLALNLWSFISYF 320
           AEKGVSLALNLW F+SYF
Sbjct: 311 AEKGVSLALNLWGFVSYF 328

BLAST of Lag0004932 vs. TAIR 10
Match: AT1G28230.1 (purine permease 1 )

HSP 1 Score: 358.6 bits (919), Expect = 5.2e-99
Identity = 187/324 (57.72%), Postives = 243/324 (75.00%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           K  L+  N ++L+IG CGGPL+  LYF +GG  +W  S+L T G+PIIL+PL +S+  RR
Sbjct: 2   KNGLIIINCIILTIGTCGGPLLTRLYFTNGGKRIWFMSFLSTAGFPIILIPLLVSFLSRR 61

Query: 66  RTAPTAS-------TKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVAC 125
           R+    +       TKL  M++ LF+AS+VIG+LT LD+YLY+YG+A L +STSSLI+  
Sbjct: 62  RSNRNPNNAENKRKTKLFLMETPLFIASIVIGLLTGLDNYLYSYGLAYLPVSTSSLIIGT 121

Query: 126 QLAFTTVFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMV 185
           QLAF  +FAFLLVKQKFT + INAVVLLTVG  +LALH+ GD+PA ES KEY+ GFLM V
Sbjct: 122 QLAFNALFAFLLVKQKFTPFSINAVVLLTVGIGILALHSDGDKPAKESKKEYVVGFLMTV 181

Query: 186 AAAVLYGFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPRE 245
            AA+LY F+LPL+ELT  KA+Q IT+ LVLEIQ+V+ L +T  C IGM I  DF+VI RE
Sbjct: 182 VAALLYAFILPLVELTYKKARQEITFPLVLEIQMVMCLAATFFCVIGMFIVGDFKVIARE 241

Query: 246 GREFGL-GETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIF 305
            REF + G   YY ++V + IIWQ FF+GA+G++F +SSL SG++I++LLP TE+ AV+ 
Sbjct: 242 AREFKIGGSVFYYALIVITGIIWQGFFLGAIGIVFCASSLASGVLISVLLPVTEVFAVVC 301

Query: 306 FRENFQAEKGVSLALNLWSFISYF 320
           FRE FQAEKGVSL L+LW F+SYF
Sbjct: 302 FREKFQAEKGVSLLLSLWGFVSYF 325

BLAST of Lag0004932 vs. TAIR 10
Match: AT1G28220.1 (purine permease 3 )

HSP 1 Score: 355.9 bits (912), Expect = 3.3e-98
Identity = 179/316 (56.65%), Postives = 243/316 (76.90%), Query Frame = 0

Query: 7   RALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRRR 66
           +AL+  N ++L+IGNCGGPLIM LYF +GG  +W S++L T G+P+I +PL  SY  RRR
Sbjct: 3   KALVIINCIILAIGNCGGPLIMRLYFNNGGKRIWFSTFLETAGFPVIFIPLLFSYITRRR 62

Query: 67  TAPTA-STKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTTV 126
           +     ST    +K RL +A+V++G+L+  D+YLYAYG+A L +ST++LI+A QLAF  +
Sbjct: 63  SNNVGDSTSFFLIKPRLLIAAVIVGILSGFDNYLYAYGIAYLPVSTAALIIASQLAFIAI 122

Query: 127 FAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLYG 186
           F+F +VK KFT + INAVVLLTVG AVL +HT  D+P  E++K+YI GFL+ VAAAV+Y 
Sbjct: 123 FSFFMVKHKFTPFTINAVVLLTVGAAVLGMHTETDKPVHETHKQYITGFLITVAAAVMYA 182

Query: 187 FVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGLG 246
           F+LPL+EL   KAKQ ++YTLVLE QL++ L ++I+  IGM I  DF+ +P+E REF LG
Sbjct: 183 FILPLVELAYQKAKQTMSYTLVLEFQLILCLLASIVSVIGMFIAGDFKALPKEAREFKLG 242

Query: 247 ETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQAE 306
           E  +YV+ V S IIWQ FF+GA+G+IF +SSL SGI+I++LLP TE++AVIF+ E FQAE
Sbjct: 243 EALFYVVAVFSAIIWQGFFLGAIGLIFSTSSLVSGIMISVLLPITEVLAVIFYHEKFQAE 302

Query: 307 KGVSLALNLWSFISYF 320
           KG+SLAL+LW F+SYF
Sbjct: 303 KGLSLALSLWGFVSYF 318

BLAST of Lag0004932 vs. TAIR 10
Match: AT2G33750.2 (purine permease 2 )

HSP 1 Score: 334.0 bits (855), Expect = 1.4e-91
Identity = 172/317 (54.26%), Postives = 234/317 (73.82%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           K  L+  N + L+IGNCGGPL+M LYF +GG  +W  S+L T G P+I  PL +S+  RR
Sbjct: 4   KTVLVIINCIFLAIGNCGGPLMMRLYFQNGGERIWFPSFLQTVGCPLIFFPLLLSFLRRR 63

Query: 66  RTAPTASTKLVF-MKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTT 125
           R      T   F MK  LF+A++V+G+L   D+YLY+YG+A + +ST+SLI++ QL FT 
Sbjct: 64  RCLEEQETTPFFLMKPPLFIAAIVVGLLVGFDNYLYSYGLAYIPVSTASLIISAQLGFTA 123

Query: 126 VFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLY 185
           +FAF +VKQKFT + INA+VLLT G  VLAL++  D+ A E++KEY+ GF+M + AA+LY
Sbjct: 124 LFAFFMVKQKFTPFTINAIVLLTGGAVVLALNSDSDKLANETHKEYVVGFIMTLGAALLY 183

Query: 186 GFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGL 245
           GF+LPL+EL+  K+ QRITYTL LE Q+V+   +T +C +GML   DF+VI  E R+F L
Sbjct: 184 GFILPLVELSYKKSGQRITYTLALEFQMVLCFAATCVCLVGMLAAGDFKVIAGEARDFKL 243

Query: 246 GETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQA 305
           GE+ YYV++V + IIWQAFFVGA+G+IF +SSL SGI+++ LLP T I+AVI F+E FQA
Sbjct: 244 GESLYYVVIVFTAIIWQAFFVGAIGLIFCASSLVSGIMVSALLPVTVILAVICFQEKFQA 303

Query: 306 EKGVSLALNLWSFISYF 320
            KGV+LAL+LW  +SYF
Sbjct: 304 GKGVALALSLWGSVSYF 320

BLAST of Lag0004932 vs. TAIR 10
Match: AT2G33750.1 (purine permease 2 )

HSP 1 Score: 325.5 bits (833), Expect = 4.8e-89
Identity = 172/328 (52.44%), Postives = 234/328 (71.34%), Query Frame = 0

Query: 6   KRALLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRR 65
           K  L+  N + L+IGNCGGPL+M LYF +GG  +W  S+L T G P+I  PL +S+  RR
Sbjct: 4   KTVLVIINCIFLAIGNCGGPLMMRLYFQNGGERIWFPSFLQTVGCPLIFFPLLLSFLRRR 63

Query: 66  RTAPTASTKLVF-MKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTT 125
           R      T   F MK  LF+A++V+G+L   D+YLY+YG+A + +ST+SLI++ QL FT 
Sbjct: 64  RCLEEQETTPFFLMKPPLFIAAIVVGLLVGFDNYLYSYGLAYIPVSTASLIISAQLGFTA 123

Query: 126 VFAFLLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLY 185
           +FAF +VKQKFT + INA+VLLT G  VLAL++  D+ A E++KEY+ GF+M + AA+LY
Sbjct: 124 LFAFFMVKQKFTPFTINAIVLLTGGAVVLALNSDSDKLANETHKEYVVGFIMTLGAALLY 183

Query: 186 GFVLPLIELT--KAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDF-----------Q 245
           GF+LPL+EL+  K+ QRITYTL LE Q+V+   +T +C +GML   DF           +
Sbjct: 184 GFILPLVELSYKKSGQRITYTLALEFQMVLCFAATCVCLVGMLAAGDFKVKHALFIFKNR 243

Query: 246 VIPREGREFGLGETKYYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEII 305
           VI  E R+F LGE+ YYV++V + IIWQAFFVGA+G+IF +SSL SGI+++ LLP T I+
Sbjct: 244 VIAGEARDFKLGESLYYVVIVFTAIIWQAFFVGAIGLIFCASSLVSGIMVSALLPVTVIL 303

Query: 306 AVIFFRENFQAEKGVSLALNLWSFISYF 320
           AVI F+E FQA KGV+LAL+LW  +SYF
Sbjct: 304 AVICFQEKFQAGKGVALALSLWGSVSYF 331

BLAST of Lag0004932 vs. TAIR 10
Match: AT2G24220.1 (purine permease 5 )

HSP 1 Score: 182.6 bits (462), Expect = 5.1e-46
Identity = 112/312 (35.90%), Postives = 176/312 (56.41%), Query Frame = 0

Query: 9   LLAFNVVLLSIGNCGGPLIMHLYFIHGGNHVWLSSWLLTGGWPIILLPLAISYFHRRRTA 68
           LL F+   + I      L+  LYF +GG   W+ SW+   GWPI  L L  +Y  ++   
Sbjct: 39  LLFFSGAAMLIAFPASSLLSRLYFSNGGKSKWIISWVAVAGWPITCLILLPTYIFQKIKP 98

Query: 69  PTASTKLVFMKSRLFLASVVIGVLTELDDYLYAYGMARLSLSTSSLIVACQLAFTTVFAF 128
              +TKLV       L+ VV+G L+  D+ +YAY  A L  STSSL+ +  LAF+ +F +
Sbjct: 99  TPLNTKLV-------LSYVVLGFLSAADNLMYAYAYAYLPASTSSLLASSSLAFSALFGY 158

Query: 129 LLVKQKFTSYPINAVVLLTVGGAVLALHTSGDRPAGESNKEYIAGFLMMVAAAVLYGFVL 188
           L+VK    +  IN++V++T   A++AL +S DR +  SN +Y AGF   +  + L+G + 
Sbjct: 159 LIVKNPLNASVINSIVVITGAMAIIALDSSSDRYSYISNSQYFAGFFWDIMGSALHGLIF 218

Query: 189 PLIEL--TKAKQRITYTLVLEIQLVISLFSTILCTIGMLINNDFQVIPREGREFGLGETK 248
            L EL   K   R ++ + LE Q+++SL +    TIGM+++NDFQ +  E + F  GE+ 
Sbjct: 219 ALSELLFVKLLGRRSFHVALEQQVMVSLTAFAFTTIGMVVSNDFQGMSHEAKSFKGGESL 278

Query: 249 YYVILVCSPIIWQAFFVGAVGVIFYSSSLFSGIVIALLLPATEIIAVIFFRENFQAEKGV 308
           Y  +LV S + +Q   +GA  V+F +S++ +G++ A+ +P T + AVI   +     K +
Sbjct: 279 YTQVLVWSAVTFQLGVLGATAVLFLASTVMAGVLNAVRVPITSVAAVILMHDPMSGFKIL 338

Query: 309 SLALNLWSFISY 319
           SL L  W F SY
Sbjct: 339 SLVLTFWGFSSY 343

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022131268.18.5e-13679.43purine permease 3-like [Momordica charantia][more]
XP_022961838.11.4e-13580.06purine permease 3-like [Cucurbita moschata][more]
KAG6598711.12.5e-13580.06Purine permease 3, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7029652.12.5e-13580.06Purine permease 3, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022997185.14.2e-13580.76purine permease 3-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FZ967.3e-9857.72Purine permease 1 OS=Arabidopsis thaliana OX=3702 GN=PUP1 PE=1 SV=1[more]
Q9FZ954.7e-9756.65Purine permease 3 OS=Arabidopsis thaliana OX=3702 GN=PUP3 PE=2 SV=1[more]
Q94GB16.8e-8852.44Purine permease 2 OS=Arabidopsis thaliana OX=3702 GN=PUP2 PE=1 SV=1[more]
Q9ZUH37.1e-4535.90Probable purine permease 5 OS=Arabidopsis thaliana OX=3702 GN=PUP5 PE=3 SV=2[more]
Q9LPF62.3e-4333.65Probable purine permease 11 OS=Arabidopsis thaliana OX=3702 GN=PUP11 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BSV44.1e-13679.43Probable purine permease OS=Momordica charantia OX=3673 GN=LOC111004539 PE=3 SV=... [more]
A0A6J1HCZ47.0e-13680.06Probable purine permease OS=Cucurbita moschata OX=3662 GN=LOC111462483 PE=3 SV=1[more]
A0A6J1K8V92.0e-13580.76Probable purine permease OS=Cucurbita maxima OX=3661 GN=LOC111492160 PE=3 SV=1[more]
A0A6J1BS924.2e-13380.70Probable purine permease OS=Momordica charantia OX=3673 GN=LOC111005083 PE=3 SV=... [more]
A0A1S3BAX31.3e-12976.73Probable purine permease OS=Cucumis melo OX=3656 GN=LOC103488092 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G28230.15.2e-9957.72purine permease 1 [more]
AT1G28220.13.3e-9856.65purine permease 3 [more]
AT2G33750.21.4e-9154.26purine permease 2 [more]
AT2G33750.14.8e-8952.44purine permease 2 [more]
AT2G24220.15.1e-4635.90purine permease 5 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF16913PUNUTcoord: 9..319
e-value: 4.2E-106
score: 354.8
NoneNo IPR availablePANTHERPTHR31376:SF1PURINE PERMEASE 2coord: 4..319
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 78..157
IPR030182Purine permease, plantPANTHERPTHR31376OS09G0467300 PROTEIN-RELATEDcoord: 4..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004932.1Lag0004932.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1904823 purine nucleobase transmembrane transport
biological_process GO:0015860 purine nucleoside transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005345 purine nucleobase transmembrane transporter activity
molecular_function GO:0015211 purine nucleoside transmembrane transporter activity