Lag0000367 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0000367
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	LINE-1 retrotransposable element ORF2 protein
Location	chr4: 5142627 .. 5142947 (+)
RNA-Seq Expression	Lag0000367
Synteny	Lag0000367

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTACACTCATCGGAAAATTACTCAATGATCATAAATGGCAAGCCAAGGGAAAAAATTATAGCTACAAGGGGTCTTAGAAAAGGAGACCCGCTTTCCCCCTTTTTATTTATCATGGTGGTTGATTGTCTAAGTAGAATGCTCCTATCGACTGATTGCACTGGCACCATTGAAGGCTTTGGAGTTGGAGATGGTCCTCAATCCTTATCAATCACTCACCTACAGTTTGCCGACGACACTATATTGTTCTCCTCCCCAAACGAAACCCATATCGTTAACCTCTTCAACATTATCCGCTCCTTTGAGACTACTTCGGGCTAA

mRNA sequence

ATGGTACACTCATCGGAAAATTACTCAATGATCATAAATGGCAAGCCAAGGGAAAAAATTATAGCTACAAGGGGTCTTAGAAAAGGAGACCCGCTTTCCCCCTTTTTATTTATCATGGTGGTTGATTGTCTAAGTAGAATGCTCCTATCGACTGATTGCACTGGCACCATTGAAGGCTTTGGAGTTGGAGATGGTCCTCAATCCTTATCAATCACTCACCTACAGTTTGCCGACGACACTATATTGTTCTCCTCCCCAAACGAAACCCATATCGTTAACCTCTTCAACATTATCCGCTCCTTTGAGACTACTTCGGGCTAA

Coding sequence (CDS)

ATGGTACACTCATCGGAAAATTACTCAATGATCATAAATGGCAAGCCAAGGGAAAAAATTATAGCTACAAGGGGTCTTAGAAAAGGAGACCCGCTTTCCCCCTTTTTATTTATCATGGTGGTTGATTGTCTAAGTAGAATGCTCCTATCGACTGATTGCACTGGCACCATTGAAGGCTTTGGAGTTGGAGATGGTCCTCAATCCTTATCAATCACTCACCTACAGTTTGCCGACGACACTATATTGTTCTCCTCCCCAAACGAAACCCATATCGTTAACCTCTTCAACATTATCCGCTCCTTTGAGACTACTTCGGGCTAA

Protein sequence

MVHSSENYSMIINGKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFGVGDGPQSLSITHLQFADDTILFSSPNETHIVNLFNIIRSFETTSG

Homology

[Close]

BLAST of Lag0000367 vs. NCBI nr
Match: XP_028121753.1 (uncharacterized protein LOC114318966 [Camellia sinensis])

HSP 1 Score: 106.3 bits (264), Expect = 1.6e-19
Identity = 55/103 (53.40%), Postives = 69/103 (66.99%), Query Frame = 0


Query: 4   SSENYSMIINGKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFGVG 63
           SS N S+I+NGK R+  +A+RGLR+GDPLSPFLFI+V D L R++      G IEGF V 
Sbjct: 352 SSCNMSVIVNGKARDSFLASRGLRQGDPLSPFLFILVTDVLGRIIDLAKDKGVIEGFVV- 411

Query: 64  DGPQSLSITHLQFADDTILFSSPNETHIVNLFNIIRSFETTSG 107
            G   + +THLQFADDTI FSS  E  +  L  ++R FE  SG
Sbjct: 412 -GRDRIEVTHLQFADDTIFFSSKEENKVGCLLQVLRIFEIVSG 452

[Close]

BLAST of Lag0000367 vs. NCBI nr
Match: RVW52726.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 99.8 bits (247), Expect = 1.5e-17
Identity = 53/103 (51.46%), Postives = 68/103 (66.02%), Query Frame = 0


Query: 4   SSENYSMIINGKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFGVG 63
           SS +Y++I+NG  +  + ATRGLR+GDPLSPFLF +V D LSRMLL  +    +EGF V 
Sbjct: 122 SSVSYAIIVNGNAKGWVKATRGLRQGDPLSPFLFTIVADVLSRMLLKAEERNLLEGFRV- 181

Query: 64  DGPQSLSITHLQFADDTILFSSPNETHIVNLFNIIRSFETTSG 107
            G     +THLQFADDTILF+SP E  +  L  ++  F   SG
Sbjct: 182 -GRNRCRVTHLQFADDTILFASPREDELQTLKCLLLVFGQISG 222

[Close]

BLAST of Lag0000367 vs. NCBI nr
Match: CAN78983.1 (hypothetical protein VITISV_038305 [Vitis vinifera])

HSP 1 Score: 99.0 bits (245), Expect = 2.6e-17
Identity = 51/103 (49.51%), Postives = 68/103 (66.02%), Query Frame = 0


Query: 4   SSENYSMIINGKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFGVG 63
           SS +Y++++NG  +  + A RGLR+GDPLSPFLF +V D LSRMLL  +    +EGF V 
Sbjct: 449 SSVSYAILVNGNAKGWVKAARGLRQGDPLSPFLFTIVADVLSRMLLKAEERNLLEGFRV- 508

Query: 64  DGPQSLSITHLQFADDTILFSSPNETHIVNLFNIIRSFETTSG 107
            G     +THLQFADDTILF+SP E  +  L +++  F   SG
Sbjct: 509 -GRNRCKVTHLQFADDTILFASPREEELQTLKSLLLVFGQISG 549

[Close]

BLAST of Lag0000367 vs. NCBI nr
Match: RVW53253.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 98.6 bits (244), Expect = 3.4e-17
Identity = 51/103 (49.51%), Postives = 68/103 (66.02%), Query Frame = 0


Query: 4   SSENYSMIINGKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFGVG 63
           SS +Y++++NG  +  + A RGLR+GDPLSPFLF +V D LSRMLL  +    +EGF V 
Sbjct: 431 SSVSYAILVNGNAKGWVKAARGLRQGDPLSPFLFTIVADVLSRMLLKAEERNLLEGFRV- 490

Query: 64  DGPQSLSITHLQFADDTILFSSPNETHIVNLFNIIRSFETTSG 107
            G     +THLQFADDTILF+SP E  +  L +++  F   SG
Sbjct: 491 -GRNRCRVTHLQFADDTILFASPREEELQTLKSLLLVFGQISG 531

[Close]

BLAST of Lag0000367 vs. NCBI nr
Match: RVW15726.1 (putative ribonuclease H protein [Vitis vinifera])

HSP 1 Score: 97.4 bits (241), Expect = 7.7e-17
Identity = 50/103 (48.54%), Postives = 68/103 (66.02%), Query Frame = 0


Query: 4   SSENYSMIINGKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFGVG 63
           SS +Y++++NG  +  + A RGLR+GDPLSPFLF +V D LSRMLL  +    +EGF V 
Sbjct: 352 SSVSYAILVNGNAKGWVKAARGLRQGDPLSPFLFTIVADVLSRMLLKAEERNLLEGFRV- 411

Query: 64  DGPQSLSITHLQFADDTILFSSPNETHIVNLFNIIRSFETTSG 107
            G     ++HLQFADDTILF+SP E  +  L +++  F   SG
Sbjct: 412 -GRNRCRVSHLQFADDTILFASPREDEVQTLKSLLLVFGQISG 452

[Close]

BLAST of Lag0000367 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 8.0e-09
Identity = 32/70 (45.71%), Postives = 42/70 (60.00%), Query Frame = 0


Query: 11 IINGKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFGVGDGPQSLS 70
          IING P+  +  +RGLR+GDPLSP+LFI+  + LS +       G + G  V +   S  
Sbjct: 13 IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSN--NSPR 72

Query: 71 ITHLQFADDT 81
          I HL FADDT
Sbjct: 73 INHLLFADDT 80

[Close]

BLAST of Lag0000367 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.1e-05
Identity = 32/105 (30.48%), Postives = 51/105 (48.57%), Query Frame = 0


Query: 2   VHSSENYSMIINGKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFG 61
           ++     ++I+NG+  E      G R+G PLSP LF +V++ L+R +        I+G  
Sbjct: 633 IYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIRQEK---EIKGIQ 692

Query: 62  VGDGPQSLSITHLQFADDTILFSSPNETHIVNLFNIIRSFETTSG 107
           +G     LS+    FADD I++         NL  +I +F   SG
Sbjct: 693 LGKEEVKLSL----FADDMIVYLENPIVSAQNLLKLISNFSKVSG 730

[Close]

BLAST of Lag0000367 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 3.1e-05
Identity = 32/105 (30.48%), Postives = 53/105 (50.48%), Query Frame = 0


Query: 2   VHSSENYSMIINGKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFG 61
           ++S    ++ +NG+  E I    G R+G PLSP+LF +V++ L+R +        I+G  
Sbjct: 640 IYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQK---EIKGIQ 699

Query: 62  VGDGPQSLSITHLQFADDTILFSSPNETHIVNLFNIIRSFETTSG 107
           +G     +S+     ADD I++ S  +     L N+I SF    G
Sbjct: 700 IGKEEVKISL----LADDMIVYISDPKNSTRELLNLINSFGEVVG 737

[Close]

BLAST of Lag0000367 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.6e-04
Identity = 29/105 (27.62%), Postives = 49/105 (46.67%), Query Frame = 0


Query: 2   VHSSENYSMIINGKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFG 61
           ++S    ++I+NG   +      G R+G PLSP LF +V++ L+  +        I+G  
Sbjct: 632 IYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIREEK---AIKGIH 691

Query: 62  VGDGPQSLSITHLQFADDTILFSSPNETHIVNLFNIIRSFETTSG 107
           +G     LS+    FADD I++          L  +I+ +   SG
Sbjct: 692 IGSEEIKLSL----FADDMIVYLENTRDSTTKLLEVIKEYSNVSG 729

[Close]

BLAST of Lag0000367 vs. ExPASy Swiss-Prot
Match: Q03274 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 4.5e-04
Identity = 29/69 (42.03%), Postives = 35/69 (50.72%), Query Frame = 0


Query: 14  GKPREKIIATRGLRKGDPLSPFLFIMVVDCLSRMLLSTDCTGTIEGFGVGDGPQSLSITH 73
           G    KI   RG+++GDPLSPFLF  V+D L   L ST         G+G       I  
Sbjct: 189 GSQTRKICIRRGVKQGDPLSPFLFNAVLDELLCSLQSTP--------GIGGTIGEEKIPV 248

Query: 74  LQFADDTIL 83
           L FADD +L
Sbjct: 249 LAFADDLLL 249

The following BLAST results are available for this feature:

BLAST of Lag0000367 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_028121753.1	1.6e-19	53.40	uncharacterized protein LOC114318966 [Camellia sinensis]	[more]
RVW52726.1	1.5e-17	51.46	LINE-1 retrotransposable element ORF2 protein [Vitis vinifera]	[more]
CAN78983.1	2.6e-17	49.51	hypothetical protein VITISV_038305 [Vitis vinifera]	[more]
RVW53253.1	3.4e-17	49.51	LINE-1 retrotransposable element ORF2 protein [Vitis vinifera]	[more]
RVW15726.1	7.7e-17	48.54	putative ribonuclease H protein [Vitis vinifera]	[more]

BLAST of Lag0000367 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P92555	8.0e-09	45.71	Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ...	[more]
O00370	3.1e-05	30.48	LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1	[more]
P11369	3.1e-05	30.48	LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE...	[more]
P08548	1.6e-04	27.62	LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1	[more]
Q03274	4.5e-04	42.03	Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 6..99 e-value: 1.5E-10 score: 41.0
IPR000477	Reverse transcriptase domain	PROSITE	PS50878	RT_POL	coord: 1..106 score: 8.711625
None	No IPR available	PANTHER	PTHR46890	NON-LTR RETROLELEMENT REVERSE TRANSCRIPTASE-LIKE PROTEIN-RELATED	coord: 6..103
None	No IPR available	PANTHER	PTHR46890:SF5	SUBFAMILY NOT NAMED	coord: 6..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0000367.1	Lag0000367.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048583	regulation of response to stimulus
cellular_component	GO:0110165	cellular anatomical entity