IVF0024089 (gene) Melon (IVF77) v1

Overview


Name	IVF0024089
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	beta-amyrin 11-oxidase-like
Location	chr04: 600470 .. 604011 (-)
RNA-Seq Expression	IVF0024089
Synteny	IVF0024089

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATGATAAGTGTTTGTGTAACTATTGGAGTTGTCTTAGGAGTTTCTTTGTTGGTGTTTAGGCGACTCAATGAATTATGGTATTTGGTTAAGTTAGGAGGAAAAGCTTACAAGTCTCTTCCTCCTGGTGACTTGGGATGGCCTGTTATTGGCTCTTCCTTCTCCTCTTACAAAACATTCATGGTTCAAGAAGATCCAATCTCTTTTATTCAATCTCTTCACTCAAGGTATCTCTTTCAATCTCTTTCTTCTTAAATCGGAAGATTCTAAGCCGATAATCATTTCGATCGGAAGAAGCAATTTTGATCTTATTTCTTAATTGTGGACTTCTTTATGCAGATATGGAGAAGGGGGAATATATAAAACTCACCTTTATGGAAAGCCAATAGTGATTGCCACAGATTCAGAAATATGTCGACGGATATACTTAGACGAAGCAAAGTTCAAACAACATAATCCAGAGTCGGTGAAAATCCTAGAAGGATCAAGTGGTGATTTCTCAAAGATGGATCACAAAATTGCATATAAAGTAGTGGCAACACCCATGAATGGGTCTGAGGTATTATCTAACTATGTGGATTTCATTGAGGAAGTAATAGCGAAAGGGTTGGAGGAATGGAGTAGCATGTTGAGAGAGCCTATTAAATTGTTGGATGAAATTGGAAGTCTCTTTTTCAAAGTCATTACAAAAGTTTTCTTAGGGTCTCGACTTGATGCCAAAACCATGGTAGAGCTTCATGCATTGTACAAAGAATTGAGTTTTGCAATGGTCATGTCCACTTTCCCTTATGACTTCCCTGGATTCACTTTTCACCAAGCACTCAAGGTACACAATTTTTTGTTTTTTCTTCTTTTAATATATATATATCAGTCCCATCTTTTCTTGACACCATCTTTGTTTTATCAGCACTCAACATCGTCTTTTTTTGTACAAAATTGTTGGTAGGCAAGAAAGAAGATAGAGAATATCATTCAAGGTGTGGTTGAGGAGAAAAGAAGAAGATTTGAAAATGATAAAACAAGTGAAGTGCAATGTCAAGTGGACAAAATGGTTGTAGCCATTGATGAGAATGGGGCAAAATTATATAATAACAATCTCATTAGAGATTTGCTTCTTGGGGTATTATTCGCTGGTCATAGTACTCCTGCCATTGCTGCTTTTTGGGCTCTGCTACATATCTCACAAAACCCTCATGTCTTCCAGAAGGCCAAGGTTAGGTTATTATATATTTTCATCCCTACACTTTCGATCAAAGGTTTAATTAATACTAAGATGTTTAATAAAATGAGTTAATTATTATTAATTAATTATTTACAGGAAGAGCAAGAGTCGATCATAAGACAAAGACCATCTAATCAAAAAGGCTTAACTCTTAACGAAATCAAACAAATGAAATATCTCACAAAGGTAACGTTTCTTTCATTTGATTTGAAGTTTACCTAATTATATCGGCGATAAGCTCTTATTTTCTTGACTTTCTAAATGATTATAAACATTTTCTTAATTCAAACCCACCCTTAATTCATCCCAATTATAACCAACCTTATTATAATTATAAATGGTAGAATCATATCACTTCTATATCAATAATTCTTAATAGAAGCCTATTAAATGATGATGACCTTGTATTACGATAAATTTCTATGATGGACAAAATTATATCACAAATTTAAGTTGTGTCATTGTAGCTAACAAATAATCCCTTCTGTCTATTAACAAAGTTGTCCATTTCTAATTGTTCACAAAAGACTAAACATGATATAAAAGATATAATGTTTAATGGAATGATTGAAACTTATAAAAAATTAGTGTTTTGTTAATGATAAACCATATCACCAATATGATCTACTAGTCGTAAGTAGAAGTACATTAGTGATAGAATCTTATACAGTAAGATAATTTCATAATTTTTAAAATTGATTCTTCAATGTCAAAAATTGTTTTTAATTGCGGTAGATTTTAATTATTGTTGTCTTGCTAATTTTCTATTGTTTTAGGTCATTAACGAAGTTTTGAGAAGAAATACAATTACTCCCACAAATTTTCGTGAGGCAAAAACAGATGTAAACATCAATGGTAAGTGTTTTCTTCTTCTTATTATTATTATTTTATGGCATATAAGTATTTAAAAATTCATTCCCATTTTATATTATACTATTGGTTCAAATTTGACTTATTTGTTTTTTTAGGGAAATTAAAACTTTTATTAAAAAGCCACAAGGGAAGAAGAATGAAAATTTTCCTTTAAGGAATCATGAGAAATTATTATTATTATTAATACATAATATTTGTTACTAATTATGTATTTAGATTGAAATTATTATTTTGTTGATGATAAATAATTAACTATTTTTTTTCCTATTGTTTTGTTTTTCTTTTTTGTTTTAGGTTATTTTATTCCAAAAGGATGGACGATACAAATTTGGAATATAGCTGTTCATATGGACCCTCAAATTCATTCTAATCCACAAGAATTTAATCCTTCAAGATGGGAAGTATGTTTTATGATTATTTTAATCAACCATTAAAATAAACTATTGTGCTTTTGAGTTTGATTCGTTTTAGTTATTAAACTTTTAAAAAGTAACCATTTAATTTCCTTTTCATTTTAAGGAGAAAAAACATATAAATCTAAATAGTAACATTTTCAAAAATACAAGAACCCAAATTGAAGTTGAGACCAAAATGAATAAAAACCAAAGGGCCGTTTGCAAAAATAGCAAAAAAAATTATGATAAGAGTGCTTATGTCATTACATTTTTTAAATTGCAAAAATATCAAATTTCAAAGTGGATAGTCCTCTTATAGCTCTTTGATAGCCCCTGATAACCGTATGATATATTTAATTACTTGTCATATTTGTAATATACAAAAAAAAAAAAAAGTGTCATGGACCATTTTTTTTCCCTAAAGATTTATTATTTGATACACAAGCACCACACATGCACACACGAGCAGGGGTGAACATCCACGCACCTTCACACAAGCAAAATGTTCTCATACTAATGGATTCGATTTTGACCTGATTCACCGTTGTTCCTCAGTAGTTTAGGACACGCACACATGTAAAAAATCTTAAGAATAGTGTTGGTGAATGCAAATATTTTCTTGAGAAGGAAAGTAGAGAGAAATAATAACAAAATTAAGAATATTAATGTTAATAGTATTTGTAAAATGTGATGGGCAGAATTATACACCAAAGCCAGGAACATTCATCCCTTTTGGACTTGGAAGTAGATTTTGTCCTGGAAGTGAACTTGCAAGGCTTGAGATTACTATTCTACTTCATCATTTCATCCTCAATTACAAGTATGTATATATATATATATATATATATATATATATATATATATATGCTTCTTCATTTTTTTTCCAAAATTGATTTATTTTATTTTGCTAAAATAAAAAAGATATTGAATTGTTATTTTGTTGGTGTATTTATTGTAGAATGGAGGGTGTCAACCTAGAAAGCTACATCACTCAAATGCCTTCCCCTCGGCCAGAAAATTGCCTTTGCAAAATTATTAGGCTGTCGTAG

mRNA sequence

ATGGAAATGATAAGTGTTTGTGTAACTATTGGAGTTGTCTTAGGAGTTTCTTTGTTGGTGTTTAGGCGACTCAATGAATTATGGTATTTGGTTAAGTTAGGAGGAAAAGCTTACAAGTCTCTTCCTCCTGGTGACTTGGGATGGCCTGTTATTGGCTCTTCCTTCTCCTCTTACAAAACATTCATGGTTCAAGAAGATCCAATCTCTTTTATTCAATCTCTTCACTCAAGATATGGAGAAGGGGGAATATATAAAACTCACCTTTATGGAAAGCCAATAGTGATTGCCACAGATTCAGAAATATGTCGACGGATATACTTAGACGAAGCAAAGTTCAAACAACATAATCCAGAGTCGGTGAAAATCCTAGAAGGATCAAGTGGTGATTTCTCAAAGATGGATCACAAAATTGCATATAAAGTAGTGGCAACACCCATGAATGGGTCTGAGGTATTATCTAACTATGTGGATTTCATTGAGGAAGTAATAGCGAAAGGGTTGGAGGAATGGAGTAGCATGTTGAGAGAGCCTATTAAATTGTTGGATGAAATTGGAAGTCTCTTTTTCAAAGTCATTACAAAAGTTTTCTTAGGGTCTCGACTTGATGCCAAAACCATGGTAGAGCTTCATGCATTGTACAAAGAATTGAGTTTTGCAATGGTCATGTCCACTTTCCCTTATGACTTCCCTGGATTCACTTTTCACCAAGCACTCAAGGCAAGAAAGAAGATAGAGAATATCATTCAAGGTGTGGTTGAGGAGAAAAGAAGAAGATTTGAAAATGATAAAACAAGTGAAGTGCAATGTCAAGTGGACAAAATGGTTGTAGCCATTGATGAGAATGGGGCAAAATTATATAATAACAATCTCATTAGAGATTTGCTTCTTGGGGTATTATTCGCTGGTCATAGTACTCCTGCCATTGCTGCTTTTTGGGCTCTGCTACATATCTCACAAAACCCTCATGTCTTCCAGAAGGCCAAGGAAGAGCAAGAGTCGATCATAAGACAAAGACCATCTAATCAAAAAGGCTTAACTCTTAACGAAATCAAACAAATGAAATATCTCACAAAGGTCATTAACGAAGTTTTGAGAAGAAATACAATTACTCCCACAAATTTTCGTGAGGCAAAAACAGATGTAAACATCAATGGTTATTTTATTCCAAAAGGATGGACGATACAAATTTGGAATATAGCTGTTCATATGGACCCTCAAATTCATTCTAATCCACAAGAATTTAATCCTTCAAGATGGGAAAATTATACACCAAAGCCAGGAACATTCATCCCTTTTGGACTTGGAAGTAGATTTTGTCCTGGAAGTGAACTTGCAAGGCTTGAGATTACTATTCTACTTCATCATTTCATCCTCAATTACAAAATGGAGGGTGTCAACCTAGAAAGCTACATCACTCAAATGCCTTCCCCTCGGCCAGAAAATTGCCTTTGCAAAATTATTAGGCTGTCGTAG

Coding sequence (CDS)

ATGGAAATGATAAGTGTTTGTGTAACTATTGGAGTTGTCTTAGGAGTTTCTTTGTTGGTGTTTAGGCGACTCAATGAATTATGGTATTTGGTTAAGTTAGGAGGAAAAGCTTACAAGTCTCTTCCTCCTGGTGACTTGGGATGGCCTGTTATTGGCTCTTCCTTCTCCTCTTACAAAACATTCATGGTTCAAGAAGATCCAATCTCTTTTATTCAATCTCTTCACTCAAGATATGGAGAAGGGGGAATATATAAAACTCACCTTTATGGAAAGCCAATAGTGATTGCCACAGATTCAGAAATATGTCGACGGATATACTTAGACGAAGCAAAGTTCAAACAACATAATCCAGAGTCGGTGAAAATCCTAGAAGGATCAAGTGGTGATTTCTCAAAGATGGATCACAAAATTGCATATAAAGTAGTGGCAACACCCATGAATGGGTCTGAGGTATTATCTAACTATGTGGATTTCATTGAGGAAGTAATAGCGAAAGGGTTGGAGGAATGGAGTAGCATGTTGAGAGAGCCTATTAAATTGTTGGATGAAATTGGAAGTCTCTTTTTCAAAGTCATTACAAAAGTTTTCTTAGGGTCTCGACTTGATGCCAAAACCATGGTAGAGCTTCATGCATTGTACAAAGAATTGAGTTTTGCAATGGTCATGTCCACTTTCCCTTATGACTTCCCTGGATTCACTTTTCACCAAGCACTCAAGGCAAGAAAGAAGATAGAGAATATCATTCAAGGTGTGGTTGAGGAGAAAAGAAGAAGATTTGAAAATGATAAAACAAGTGAAGTGCAATGTCAAGTGGACAAAATGGTTGTAGCCATTGATGAGAATGGGGCAAAATTATATAATAACAATCTCATTAGAGATTTGCTTCTTGGGGTATTATTCGCTGGTCATAGTACTCCTGCCATTGCTGCTTTTTGGGCTCTGCTACATATCTCACAAAACCCTCATGTCTTCCAGAAGGCCAAGGAAGAGCAAGAGTCGATCATAAGACAAAGACCATCTAATCAAAAAGGCTTAACTCTTAACGAAATCAAACAAATGAAATATCTCACAAAGGTCATTAACGAAGTTTTGAGAAGAAATACAATTACTCCCACAAATTTTCGTGAGGCAAAAACAGATGTAAACATCAATGGTTATTTTATTCCAAAAGGATGGACGATACAAATTTGGAATATAGCTGTTCATATGGACCCTCAAATTCATTCTAATCCACAAGAATTTAATCCTTCAAGATGGGAAAATTATACACCAAAGCCAGGAACATTCATCCCTTTTGGACTTGGAAGTAGATTTTGTCCTGGAAGTGAACTTGCAAGGCTTGAGATTACTATTCTACTTCATCATTTCATCCTCAATTACAAAATGGAGGGTGTCAACCTAGAAAGCTACATCACTCAAATGCCTTCCCCTCGGCCAGAAAATTGCCTTTGCAAAATTATTAGGCTGTCGTAG

Protein sequence

MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESVKILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKARKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLFAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVINEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPRPENCLCKIIRLS

Homology

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BLAST of IVF0024089 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 3.5e-115
Identity = 221/498 (44.38%), Postives = 327/498 (65.66%), Query Frame = 0


Query: 1   MEMISVCVTIGVVLGVSLL---VFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSS 60
           ME+  VC++   +L   +      R LN  +Y VKL  K +  LPPGD+GWP+IG   S 
Sbjct: 1   MEVHWVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEH-PLPPGDMGWPLIGDLLSF 60

Query: 61  YKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNP 120
            K F     P SFI +L  +YG  GIYKTHL+G P +I  + ++CRR+  D+  FK   P
Sbjct: 61  IKDFS-SGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYP 120

Query: 121 ESVKILEGSSG--DFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLR 180
           +S+K L       D S  +H++  +++ +P+ G + L+ Y++ +EE++   LEE SSM +
Sbjct: 121 KSIKELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSM-K 180

Query: 181 EPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFH 240
            P++LL E+  + FK I  VF+GS  +   + ++ + + +L   M   + P + PGFTFH
Sbjct: 181 HPVELLKEMKKVSFKAIVHVFMGSS-NQDIIKKIGSSFTDLYNGMF--SIPINVPGFTFH 240

Query: 241 QALKARKKIENIIQGVVEEKRRRFEN--DKTSEVQCQVDKMVVAIDENGAKLYNNNLIRD 300
           +AL+ARKK+  I+Q VV+E+R   EN   + S+ +  +D ++   DENG KL + + I D
Sbjct: 241 KALEARKKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDED-ISD 300

Query: 301 LLLGVLFAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQM 360
           LL+G+LFAGH + A +  W++ +++Q+PH+ +KAKEEQE I R R S+QK L+L EIKQM
Sbjct: 301 LLIGLLFAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQM 360

Query: 361 KYLTKVINEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQE 420
            YL++VI+E LR   I    FREA  DVNINGY IPKGW + IW  A+HMD + + NP+E
Sbjct: 361 VYLSQVIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEE 420

Query: 421 FNPSRWENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQ 480
           FNPSRW++Y  K GTF+PFG GSR CPG++LA+LEI+I LH+F+ NY++E +N E ++T 
Sbjct: 421 FNPSRWDDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECHVTS 480

Query: 481 MPSPRP-ENCLCKIIRLS 491
           +P  +P +NCL K+I++S
Sbjct: 481 LPVSKPTDNCLAKVIKVS 491

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BLAST of IVF0024089 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 378.3 bits (970), Expect = 1.4e-103
Identity = 212/482 (43.98%), Postives = 299/482 (62.03%), Query Frame = 0


Query: 12  VVLGVSLL--VFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPIS 71
           ++LG+ +L  V +R+N   Y+ KLG K +  LPPGDLGWPVIG+ +S  + F    DP S
Sbjct: 14  IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 73

Query: 72  FIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESVKILEG--SS 131
           FIQS  +RYG  GIYK H++G P V+ T  E CRR+  D+  F    P+S   L G  S 
Sbjct: 74  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133

Query: 132 GDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSL 191
              S  +HK   ++ + P+NG E LS Y+ FIEE +   LE+WS M    I+ L  +  L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193

Query: 192 FFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKARKKIENI 251
            FKVI  +FL S  +   M  L   Y  L++   +     + PGF +H+ALKARKK+   
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYG--VRAMGINLPGFAYHRALKARKKLVAA 253

Query: 252 IQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLFAGHSTPA 311
            Q +V  +R + + + +S  +  +D ++   DENG  L +  +I DLLL  L AGH +  
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEII-DLLLMYLNAGHESSG 313

Query: 312 IAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVINEVLRRN 371
               WA + + ++P + QKAKEEQE I+++R   QK LTL E ++M YL++VI+E LR  
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373

Query: 372 TITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWENYTPKPG 431
           T + T FREAK+DV ++GY IPKGW +  W   VH+DP+I+ +P++F+PSRWE YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433

Query: 432 TFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKI 489
           TF+PFGLGS  CPG++LA+LEI+I LHHF+L Y++E  N    +  +P  RP +NCL +I
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486

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BLAST of IVF0024089 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 3.5e-99
Identity = 184/492 (37.40%), Postives = 304/492 (61.79%), Query Frame = 0


Query: 3   MISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKTFM 62
           +++  V  GVVL  +  V RR ++   +  LG +    LPPG++GWP++GS ++  + F 
Sbjct: 19  VLAAAVVAGVVLVDA--VVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFK 78

Query: 63  VQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESVKI 122
              +P +FI S   R+G  G+Y+T ++  P ++A   E C+++ +D+  F    P++   
Sbjct: 79  -SGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVT 138

Query: 123 LEG--SSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 182
           L G  S  + S  DH+   K+ A P+NG + L+ Y+ FI++ +   L  WSS     ++ 
Sbjct: 139 LIGPKSFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEF 198

Query: 183 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 242
           L E+  + FK+I ++F+    DA TM  L   Y +L++   M     + PGF +++AL+A
Sbjct: 199 LTELRRMTFKIIVQIFMSGADDA-TMEALERSYTDLNYG--MRAMAINLPGFAYYRALRA 258

Query: 243 RKKIENIIQGVVEEKR-RRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVL 302
           R+K+ +++QGV++ +R    +  K S     +D+++ A DE G +L ++ ++ D+L+  L
Sbjct: 259 RRKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIV-DVLIMYL 318

Query: 303 FAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKV 362
            AGH +      WA + + +NP +F +AK EQE I+R  P+ Q GLTL + K+M +L++V
Sbjct: 319 NAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQV 378

Query: 363 INEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRW 422
           ++E LR   I+  +FR+A  D+ +NGY IPKGW +Q+W  +VHMD Q++ +P+ FNPSRW
Sbjct: 379 VDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRW 438

Query: 423 ENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPRP 482
           E   PK GTF+PFGLG+R CPG++LA+LEI++ LHHF+L YK++  N +  +  +P PRP
Sbjct: 439 EGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRP 498

Query: 483 -ENCLCKIIRLS 491
            +NCL  I ++S
Sbjct: 499 VDNCLATITKVS 503

[Close]

BLAST of IVF0024089 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 5.5e-97
Identity = 185/495 (37.37%), Postives = 295/495 (59.60%), Query Frame = 0


Query: 5   SVCVTIGVVLGVSLLVFRRLNELWY-LVKLGGKAYKSLPPGDLGWPVIGSSFSSYKTFMV 64
           +V   +  +  V+L    R    WY    LG      LPPGD+GWP++G  ++  + F  
Sbjct: 8   AVAAVVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFK- 67

Query: 65  QEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESVKIL 124
              P SFI S   R+G  G+Y+  ++  P ++AT  E C+++ +D+  F    P++   L
Sbjct: 68  SGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVAL 127

Query: 125 EG--SSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLRE-PIKL 184
            G  S  +    +H+   K+ A P+NG + L++Y+ FI++ +   L  WS    +   + 
Sbjct: 128 IGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEF 187

Query: 185 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 244
           L E+  + F++I ++F+G   D +T  EL   Y EL++   M     D PGF +H+A++A
Sbjct: 188 LTELRRMTFRIIVQIFMGG-ADERTAAELERTYTELNYG--MRAMAIDLPGFAYHKAIRA 247

Query: 245 RKKIENIIQGVVEEKRRRFENDKTSEVQC----QVDKMVVAIDENGAKLYNNNLIRDLLL 304
           R+++   +Q V++E+R R    KT+         +D+++   DE G +L ++ +I D+L+
Sbjct: 248 RRRLVAALQRVLDERRAR--GGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEII-DVLV 307

Query: 305 GVLFAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYL 364
             L AGH +      WA + + +NP +  KAK EQE+I+R  P  QKGLTL + ++M YL
Sbjct: 308 MYLNAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYL 367

Query: 365 TKVINEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNP 424
           ++V++E LR   I+  +FR+A  DV +NGY IPKGW +Q+W  +VHMDPQ++ +P++F+P
Sbjct: 368 SQVVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDP 427

Query: 425 SRWENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPS 484
           SRWE   P+ GTF+PFGLG+R CPG++LA+LEI++ LHHF+L YK+   N    +  +P 
Sbjct: 428 SRWEGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLPH 487

Query: 485 PRP-ENCLCKIIRLS 491
           PRP +NCL KI RLS
Sbjct: 488 PRPVDNCLAKITRLS 495

[Close]

BLAST of IVF0024089 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.6e-96
Identity = 193/476 (40.55%), Postives = 287/476 (60.29%), Query Frame = 0


Query: 15  GVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPISFIQSL 74
           G++ LV R++N   Y   LG   +  LPPGDLGWP IG+  S  + F    DP SF ++L
Sbjct: 21  GLNWLV-RKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTL 80

Query: 75  HSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESVKILEG--SSGDFSK 134
             RYG  GIYK H++G P +I T S+ CRR+  D+  FK   P S   L G  S    S 
Sbjct: 81  IKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISF 140

Query: 135 MDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSLFFKVI 194
            +HK   ++ A P+NG E LS Y+ +IEE +   L++W+ M     + L  +  L F++I
Sbjct: 141 EEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM--GEFEFLTHLRKLTFRII 200

Query: 195 TKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKARKKIENIIQGVV 254
             +FL S  +   M  L   Y  L++   +     + PGF +H+ALKARK +    Q +V
Sbjct: 201 MYIFLSSESE-NVMDALEREYTALNYG--VRAMAVNIPGFAYHRALKARKTLVAAFQSIV 260

Query: 255 EEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLFAGHSTPAIAAFW 314
            E+R + + +  S  +  +D ++   DE+G  L +  +I D+LL  L AGH +      W
Sbjct: 261 TERRNQRKQNILSNKKDMLDNLLNVKDEDGKTLDDEEII-DVLLMYLNAGHESSGHTIMW 320

Query: 315 ALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVINEVLRRNTITPT 374
           A + + ++P V Q+AK EQE I++ RP  QKGL+L E ++M++L++V++E LR  T + T
Sbjct: 321 ATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLT 380

Query: 375 NFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWEN-YTPKPGTFIP 434
            FREAKTDV +NGY IPKGW +  W   VH+DP++  +P++F+P+RW+N + PK G F+P
Sbjct: 381 AFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLP 440

Query: 435 FGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKI 487
           FG GS  CPG++LA+LEI+I LHHF+L Y+++  N E  +  +P  RP +NCL +I
Sbjct: 441 FGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of IVF0024089 vs. ExPASy TrEMBL
Match: A0A1S3CRX8 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103504002 PE=3 SV=1)

HSP 1 Score: 923.3 bits (2385), Expect = 4.2e-265
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0


Query: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
           MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT
Sbjct: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60

Query: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
           FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV
Sbjct: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120

Query: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
           KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL
Sbjct: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180

Query: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
           LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA
Sbjct: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300
           RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF
Sbjct: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300

Query: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360
           AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI
Sbjct: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360

Query: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420
           NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE
Sbjct: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 460
           NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN
Sbjct: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 459

[Close]

BLAST of IVF0024089 vs. ExPASy TrEMBL
Match: A0A097IYP6 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.8e-255
Identity = 438/490 (89.39%), Postives = 462/490 (94.29%), Query Frame = 0


Query: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
           MEMISVCV IGVVLGV LLV RRLNELWYLVKLGG+AYKSLPPGDLGWPVIG SFSSYKT
Sbjct: 1   MEMISVCVIIGVVLGVCLLV-RRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKT 60

Query: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
           F+VQEDPISFIQSLHSRYG+GG+YKTHLYG P VI T+ EICRRIYLDEA FKQH P+SV
Sbjct: 61  FIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSV 120

Query: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
           KILEGSSGDFS MDHKIAYKV+A+PMNG EVLSNYVDFIEEVIAKGLEEWSSM+REPIKL
Sbjct: 121 KILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKL 180

Query: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
           +DEIG LFFKVITK+FLGS+LDAKTMVELH LYKELSF MVMSTFPYDFPGFTFHQ LKA
Sbjct: 181 VDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300
           R+KIEN IQGVVEEK RRFENDKTSEVQCQVDK+VVAI+ENGAKLY+NN IRDL+LG+ F
Sbjct: 241 RRKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFF 300

Query: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360
           AGHSTPAIAA WALLHISQNPHVFQKAKEEQESIIRQRPS QKGLTLNEIKQMKYLTK+I
Sbjct: 301 AGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKII 360

Query: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420
           NEVLRRNTIT TNFRE KTDVNING+FIPKGWT+QIWNIAVHMDPQI+SNPQEFNPSRW+
Sbjct: 361 NEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWD 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPRPE 480
           NYTPKPG FIPFGLGSRFCPGSELA+LEITILLHHFILNYKMEGVNLES   QMPSPRPE
Sbjct: 421 NYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRPE 480

Query: 481 NCLCKIIRLS 491
           NCLCKIIRLS
Sbjct: 481 NCLCKIIRLS 489

[Close]

BLAST of IVF0024089 vs. ExPASy TrEMBL
Match: A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 3.9e-186
Identity = 329/490 (67.14%), Postives = 396/490 (80.82%), Query Frame = 0


Query: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
           ME I+VCV IG VLGV + V R+LNE+WYLVKLG KAYKSLPPGD+GWP++GSSFS Y  
Sbjct: 1   METINVCVIIGFVLGVYMFV-RKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNA 60

Query: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
           F V  DP SFI  L SR G  G+YK+H YG+P +I TD EICRRIYLD+  FK + P+SV
Sbjct: 61  FTVGGDPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSV 120

Query: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
           KILE  +G F++ DHK AY+++A PMNG EVLS+YVDFI++V+AKGLEEWSSM +EPI+L
Sbjct: 121 KILE-VNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIEL 180

Query: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
           L EIGSLFFKVI+++FLG+ + A  M EL  LYK+L  A ++S  PYD PG TFH ALKA
Sbjct: 181 LGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPA-ILSILPYDLPGLTFHGALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300
           RKKIENI+Q V+EEKRR  E ++  EV+ Q+DK+++A DENG KLY+NN I DLLLG+L 
Sbjct: 241 RKKIENILQCVIEEKRRALEKEE-REVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLH 300

Query: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360
           AGH TPA AA WAL+HIS+ PHVF KAKEEQESII+QRPS QKGLT  EIKQMKYL K I
Sbjct: 301 AGHHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFI 360

Query: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420
           NE+LRRNTI   +FR+AK DVNIN Y IPKGW + IW  A+HMDPQ++SNPQEF+PSRWE
Sbjct: 361 NELLRRNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWE 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPR-P 480
           NY+PKPGTF+PFG+G+RFCPGSEL +LEITILLHHFILNYKME VN +  IT +P+P+  
Sbjct: 421 NYSPKPGTFLPFGIGTRFCPGSELTKLEITILLHHFILNYKMERVNPKCCITHLPAPKLV 480

Query: 481 ENCLCKIIRL 490
           +NCLC I +L
Sbjct: 481 DNCLCTITKL 485

[Close]

BLAST of IVF0024089 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 5.1e-186
Identity = 323/490 (65.92%), Postives = 397/490 (81.02%), Query Frame = 0


Query: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
           MEMI+VC  IG VLGV + V R+LNE+WYLVKLG KAYKSLPPGD+GWP++GSSFS Y  
Sbjct: 1   MEMINVCTIIGAVLGVYMFV-RKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNA 60

Query: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
           F V  DP SFI  L SR G  G+YK+HLYG+P +I TD EICRRIYLD+  FK + P+SV
Sbjct: 61  FTVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSV 120

Query: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
           KILE  +G F++ DHK AY+++A PMNG EVLS+YVDFI++V+AKGLEEWSSM +EPI+L
Sbjct: 121 KILE-VNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIEL 180

Query: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
           L EIGSLFFKVI+++FLG+ + A  M EL  LYK+L  A ++S  PYD PG  +H+ALKA
Sbjct: 181 LGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPA-ILSILPYDVPGLVYHRALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300
           RKKIEN+++ V+EEKRR  E  +T EV  Q+DK+++A DENG KLY+NN I DLLLG+L 
Sbjct: 241 RKKIENVLECVIEEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLH 300

Query: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360
           AGH TPA AA WAL+HIS+ PH+F KAKEEQESI++QRPS QKGLT  EIKQMKYL K I
Sbjct: 301 AGHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFI 360

Query: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420
           NE+LRRNTI   +FR+AKTD+NIN Y IPKGW++ IW  AVHMDPQ++SNP+EF+PSRWE
Sbjct: 361 NELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWE 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPR-P 480
           N++PKPGTFIPFG+G+RFCPGSEL +LEITILLHHFILNYKME  N +  I+ +P+P+  
Sbjct: 421 NHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYKMERTNPKCCISHLPAPKLV 480

Query: 481 ENCLCKIIRL 490
           +NCLC I +L
Sbjct: 481 DNCLCTITKL 486

[Close]

BLAST of IVF0024089 vs. ExPASy TrEMBL
Match: A0A5D3E6B2 (Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004210 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.8e-183
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
           MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT
Sbjct: 12  MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 71

Query: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
           FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV
Sbjct: 72  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 131

Query: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
           KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL
Sbjct: 132 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 191

Query: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
           LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA
Sbjct: 192 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 251

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300
           RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF
Sbjct: 252 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 311

Query: 301 AGHSTPAIAAFWALLHISQNPHVFQKAK 329
           AGHSTPAIAAFWALLHISQNPHVFQKAK
Sbjct: 312 AGHSTPAIAAFWALLHISQNPHVFQKAK 339

[Close]

BLAST of IVF0024089 vs. NCBI nr
Match: XP_008466645.1 (PREDICTED: beta-amyrin 11-oxidase-like, partial [Cucumis melo])

HSP 1 Score: 920 bits (2378), Expect = 0.0
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0


Query: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
           MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT
Sbjct: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60

Query: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
           FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV
Sbjct: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120

Query: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
           KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL
Sbjct: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180

Query: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
           LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA
Sbjct: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300
           RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF
Sbjct: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300

Query: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360
           AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI
Sbjct: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360

Query: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420
           NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE
Sbjct: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 459
           NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN
Sbjct: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 459

[Close]

BLAST of IVF0024089 vs. NCBI nr
Match: XP_004148515.2 (beta-amyrin 11-oxidase [Cucumis sativus] >AIT72036.1 cytochrome P450 [Cucumis sativus] >KGN60415.1 hypothetical protein Csa_001840 [Cucumis sativus])

HSP 1 Score: 887 bits (2293), Expect = 0.0
Identity = 438/490 (89.39%), Postives = 462/490 (94.29%), Query Frame = 0


Query: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
           MEMISVCV IGVVLGV LLV RRLNELWYLVKLGG+AYKSLPPGDLGWPVIG SFSSYKT
Sbjct: 1   MEMISVCVIIGVVLGVCLLV-RRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKT 60

Query: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
           F+VQEDPISFIQSLHSRYG+GG+YKTHLYG P VI T+ EICRRIYLDEA FKQH P+SV
Sbjct: 61  FIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSV 120

Query: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
           KILEGSSGDFS MDHKIAYKV+A+PMNG EVLSNYVDFIEEVIAKGLEEWSSM+REPIKL
Sbjct: 121 KILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKL 180

Query: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
           +DEIG LFFKVITK+FLGS+LDAKTMVELH LYKELSF MVMSTFPYDFPGFTFHQ LKA
Sbjct: 181 VDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300
           R+KIEN IQGVVEEK RRFENDKTSEVQCQVDK+VVAI+ENGAKLY+NN IRDL+LG+ F
Sbjct: 241 RRKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFF 300

Query: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360
           AGHSTPAIAA WALLHISQNPHVFQKAKEEQESIIRQRPS QKGLTLNEIKQMKYLTK+I
Sbjct: 301 AGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKII 360

Query: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420
           NEVLRRNTIT TNFRE KTDVNING+FIPKGWT+QIWNIAVHMDPQI+SNPQEFNPSRW+
Sbjct: 361 NEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWD 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPRPE 480
           NYTPKPG FIPFGLGSRFCPGSELA+LEITILLHHFILNYKMEGVNLES   QMPSPRPE
Sbjct: 421 NYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRPE 480

Query: 481 NCLCKIIRLS 490
           NCLCKIIRLS
Sbjct: 481 NCLCKIIRLS 489

[Close]

BLAST of IVF0024089 vs. NCBI nr
Match: XP_011652660.1 (beta-amyrin 11-oxidase [Cucumis sativus])

HSP 1 Score: 694 bits (1791), Expect = 3.11e-247
Identity = 339/493 (68.76%), Postives = 410/493 (83.16%), Query Frame = 0


Query: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
           ME+++VCV IGVVLG  + V RR NE+WYL KLG K  KSLPPGDLGWP +GS+FS YK 
Sbjct: 1   MELMNVCVIIGVVLGFYVFV-RRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKA 60

Query: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
           F V+ DP +FI +L  RYG  G+YK+HLYG P +I T+ EICRRIYLD+ +F+ + P+SV
Sbjct: 61  FKVEGDPYTFIHTLLLRYGRVGMYKSHLYGMPTIIVTNPEICRRIYLDDERFEPNYPKSV 120

Query: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
           KILE ++GDFSK+DHK  YK++A+PMNGSEVLS +V+FIE+ + KGLEEWSSM REPI+L
Sbjct: 121 KILE-TNGDFSKIDHKSGYKIMASPMNGSEVLSKHVEFIEQTVEKGLEEWSSMRREPIEL 180

Query: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
           +DEIG L FK+I  +FLG+ +D + M ELH LYKEL   ++MS  PYD PGFT+ +ALKA
Sbjct: 181 VDEIGGLLFKIILHIFLGNEIDGQAMAELHTLYKELGL-VIMSFLPYDLPGFTYRRALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDK-TSEV-QCQVDKMVVAIDENGAKLYNNNLIRDLLLGV 300
           RKKIE I+  V+E+KR+RFE D  T+EV  CQVDK++VA +ENG+K YNN+ I DL+LG+
Sbjct: 241 RKKIEKILHCVIEKKRKRFEKDDGTNEVVHCQVDKLIVATNENGSKPYNNSTIIDLILGI 300

Query: 301 LFAGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTK 360
            FAGH+TPAIAA WALLHISQNPH+FQ AKEEQESIIRQRPS QKGLT  EIKQMKYLTK
Sbjct: 301 FFAGHNTPAIAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKYLTK 360

Query: 361 VINEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSR 420
            INEVLRRNT+ PTNFR+A+T VNINGY IPKGWT+QIW++A+HMD QI+SN QEF+PSR
Sbjct: 361 FINEVLRRNTVAPTNFRKARTYVNINGYTIPKGWTVQIWSVAIHMDSQIYSNSQEFDPSR 420

Query: 421 WENYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPR 480
           W+NYTPKPG FIPFGLGSRFCPGSELA+LEITILLHHFILNYKME V+    +T +PSP+
Sbjct: 421 WDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMELVDQNCKVTHLPSPK 480

Query: 481 P-ENCLCKIIRLS 490
           P +NCLCKIIR+S
Sbjct: 481 PRDNCLCKIIRVS 490

[Close]

BLAST of IVF0024089 vs. NCBI nr
Match: KAE8651450.1 (hypothetical protein Csa_001467 [Cucumis sativus])

HSP 1 Score: 679 bits (1752), Expect = 4.16e-233
Identity = 327/472 (69.28%), Postives = 394/472 (83.47%), Query Frame = 0


Query: 22   RRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPISFIQSLHSRYGEG 81
            RR NE+WYL KLG K  KSLPPGDLGWP +GS+FS YK F V+ DP +FI +L  RYG  
Sbjct: 587  RRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKAFKVEGDPYTFIHTLLLRYGRV 646

Query: 82   GIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESVKILEGSSGDFSKMDHKIAYKV 141
            G+YK+HLYG P +I T+ EICRRIYLD+ +F+ + P+SVKILE ++GDFSK+DHK  YK+
Sbjct: 647  GMYKSHLYGMPTIIVTNPEICRRIYLDDERFEPNYPKSVKILE-TNGDFSKIDHKSGYKI 706

Query: 142  VATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSLFFKVITKVFLGSRL 201
            +A+PMNGSEVLS +V+FIE+ + KGLEEWSSM REPI+L+DEIG L FK+I  +FLG+ +
Sbjct: 707  MASPMNGSEVLSKHVEFIEQTVEKGLEEWSSMRREPIELVDEIGGLLFKIILHIFLGNEI 766

Query: 202  DAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKARKKIENIIQGVVEEKRRRFEN 261
            D + M ELH LYKEL   ++MS  PYD PGFT+ +ALKARKKIE I+  V+E+KR+RFE 
Sbjct: 767  DGQAMAELHTLYKELGL-VIMSFLPYDLPGFTYRRALKARKKIEKILHCVIEKKRKRFEK 826

Query: 262  DK-TSEV-QCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLFAGHSTPAIAAFWALLHISQ 321
            D  T+EV  CQVDK++VA +ENG+K YNN+ I DL+LG+ FAGH+TPAIAA WALLHISQ
Sbjct: 827  DDGTNEVVHCQVDKLIVATNENGSKPYNNSTIIDLILGIFFAGHNTPAIAAMWALLHISQ 886

Query: 322  NPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVINEVLRRNTITPTNFREAKT 381
            NPH+FQ AKEEQESIIRQRPS QKGLT  EIKQMKYLTK INEVLRRNT+ PTNFR+A+T
Sbjct: 887  NPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKYLTKFINEVLRRNTVAPTNFRKART 946

Query: 382  DVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWENYTPKPGTFIPFGLGSRFC 441
             VNINGY IPKGWT+QIW++A+HMD QI+SN QEF+PSRW+NYTPKPG FIPFGLGSRFC
Sbjct: 947  YVNINGYTIPKGWTVQIWSVAIHMDSQIYSNSQEFDPSRWDNYTPKPGEFIPFGLGSRFC 1006

Query: 442  PGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKIIRLS 490
            PGSELA+LEITILLHHFILNYKME V+    +T +PSP+P +NCLCKIIR+S
Sbjct: 1007 PGSELAKLEITILLHHFILNYKMELVDQNCKVTHLPSPKPRDNCLCKIIRVS 1056

[Close]

BLAST of IVF0024089 vs. NCBI nr
Match: XP_022952843.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 657 bits (1695), Expect = 1.07e-232
Identity = 329/490 (67.14%), Postives = 396/490 (80.82%), Query Frame = 0


Query: 1   MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
           ME I+VCV IG VLGV + V R+LNE+WYLVKLG KAYKSLPPGD+GWP++GSSFS Y  
Sbjct: 1   METINVCVIIGFVLGVYMFV-RKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNA 60

Query: 61  FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
           F V  DP SFI  L SR G  G+YK+H YG+P +I TD EICRRIYLD+  FK + P+SV
Sbjct: 61  FTVGGDPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSV 120

Query: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
           KILE  +G F++ DHK AY+++A PMNG EVLS+YVDFI++V+AKGLEEWSSM +EPI+L
Sbjct: 121 KILE-VNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIEL 180

Query: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
           L EIGSLFFKVI+++FLG+ + A  M EL  LYK+L  A ++S  PYD PG TFH ALKA
Sbjct: 181 LGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPA-ILSILPYDLPGLTFHGALKA 240

Query: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300
           RKKIENI+Q V+EEKRR  E ++  EV+ Q+DK+++A DENG KLY+NN I DLLLG+L 
Sbjct: 241 RKKIENILQCVIEEKRRALEKEER-EVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLH 300

Query: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360
           AGH TPA AA WAL+HIS+ PHVF KAKEEQESII+QRPS QKGLT  EIKQMKYL K I
Sbjct: 301 AGHHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFI 360

Query: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420
           NE+LRRNTI   +FR+AK DVNIN Y IPKGW + IW  A+HMDPQ++SNPQEF+PSRWE
Sbjct: 361 NELLRRNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWE 420

Query: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPR-P 480
           NY+PKPGTF+PFG+G+RFCPGSEL +LEITILLHHFILNYKME VN +  IT +P+P+  
Sbjct: 421 NYSPKPGTFLPFGIGTRFCPGSELTKLEITILLHHFILNYKMERVNPKCCITHLPAPKLV 480

Query: 481 ENCLCKIIRL 489
           +NCLC I +L
Sbjct: 481 DNCLCTITKL 485

[Close]

BLAST of IVF0024089 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 378.3 bits (970), Expect = 9.7e-105
Identity = 212/482 (43.98%), Postives = 299/482 (62.03%), Query Frame = 0


Query: 12  VVLGVSLL--VFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPIS 71
           ++LG+ +L  V +R+N   Y+ KLG K +  LPPGDLGWPVIG+ +S  + F    DP S
Sbjct: 14  IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 73

Query: 72  FIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESVKILEG--SS 131
           FIQS  +RYG  GIYK H++G P V+ T  E CRR+  D+  F    P+S   L G  S 
Sbjct: 74  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133

Query: 132 GDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSL 191
              S  +HK   ++ + P+NG E LS Y+ FIEE +   LE+WS M    I+ L  +  L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193

Query: 192 FFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKARKKIENI 251
            FKVI  +FL S  +   M  L   Y  L++   +     + PGF +H+ALKARKK+   
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYG--VRAMGINLPGFAYHRALKARKKLVAA 253

Query: 252 IQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLFAGHSTPA 311
            Q +V  +R + + + +S  +  +D ++   DENG  L +  +I DLLL  L AGH +  
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEII-DLLLMYLNAGHESSG 313

Query: 312 IAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVINEVLRRN 371
               WA + + ++P + QKAKEEQE I+++R   QK LTL E ++M YL++VI+E LR  
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373

Query: 372 TITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWENYTPKPG 431
           T + T FREAK+DV ++GY IPKGW +  W   VH+DP+I+ +P++F+PSRWE YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433

Query: 432 TFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKI 489
           TF+PFGLGS  CPG++LA+LEI+I LHHF+L Y++E  N    +  +P  RP +NCL +I
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486

[Close]

BLAST of IVF0024089 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 378.3 bits (970), Expect = 9.7e-105
Identity = 212/482 (43.98%), Postives = 299/482 (62.03%), Query Frame = 0


Query: 12  VVLGVSLL--VFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPIS 71
           ++LG+ +L  V +R+N   Y+ KLG K +  LPPGDLGWPVIG+ +S  + F    DP S
Sbjct: 14  IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 73

Query: 72  FIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESVKILEG--SS 131
           FIQS  +RYG  GIYK H++G P V+ T  E CRR+  D+  F    P+S   L G  S 
Sbjct: 74  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133

Query: 132 GDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSL 191
              S  +HK   ++ + P+NG E LS Y+ FIEE +   LE+WS M    I+ L  +  L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193

Query: 192 FFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKARKKIENI 251
            FKVI  +FL S  +   M  L   Y  L++   +     + PGF +H+ALKARKK+   
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYG--VRAMGINLPGFAYHRALKARKKLVAA 253

Query: 252 IQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLFAGHSTPA 311
            Q +V  +R + + + +S  +  +D ++   DENG  L +  +I DLLL  L AGH +  
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEII-DLLLMYLNAGHESSG 313

Query: 312 IAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVINEVLRRN 371
               WA + + ++P + QKAKEEQE I+++R   QK LTL E ++M YL++VI+E LR  
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373

Query: 372 TITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWENYTPKPG 431
           T + T FREAK+DV ++GY IPKGW +  W   VH+DP+I+ +P++F+PSRWE YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433

Query: 432 TFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKI 489
           TF+PFGLGS  CPG++LA+LEI+I LHHF+L Y++E  N    +  +P  RP +NCL +I
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486

[Close]

BLAST of IVF0024089 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 353.6 bits (906), Expect = 2.6e-97
Identity = 193/476 (40.55%), Postives = 287/476 (60.29%), Query Frame = 0


Query: 15  GVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPISFIQSL 74
           G++ LV R++N   Y   LG   +  LPPGDLGWP IG+  S  + F    DP SF ++L
Sbjct: 21  GLNWLV-RKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAFKT-SDPDSFTRTL 80

Query: 75  HSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESVKILEG--SSGDFSK 134
             RYG  GIYK H++G P +I T S+ CRR+  D+  FK   P S   L G  S    S 
Sbjct: 81  IKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISF 140

Query: 135 MDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSLFFKVI 194
            +HK   ++ A P+NG E LS Y+ +IEE +   L++W+ M     + L  +  L F++I
Sbjct: 141 EEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM--GEFEFLTHLRKLTFRII 200

Query: 195 TKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKARKKIENIIQGVV 254
             +FL S  +   M  L   Y  L++   +     + PGF +H+ALKARK +    Q +V
Sbjct: 201 MYIFLSSESE-NVMDALEREYTALNYG--VRAMAVNIPGFAYHRALKARKTLVAAFQSIV 260

Query: 255 EEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLFAGHSTPAIAAFW 314
            E+R + + +  S  +  +D ++   DE+G  L +  +I D+LL  L AGH +      W
Sbjct: 261 TERRNQRKQNILSNKKDMLDNLLNVKDEDGKTLDDEEII-DVLLMYLNAGHESSGHTIMW 320

Query: 315 ALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVINEVLRRNTITPT 374
           A + + ++P V Q+AK EQE I++ RP  QKGL+L E ++M++L++V++E LR  T + T
Sbjct: 321 ATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLT 380

Query: 375 NFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWEN-YTPKPGTFIP 434
            FREAKTDV +NGY IPKGW +  W   VH+DP++  +P++F+P+RW+N + PK G F+P
Sbjct: 381 AFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLP 440

Query: 435 FGLGSRFCPGSELARLEITILLHHFILNYKMEGVNLESYITQMPSPRP-ENCLCKI 487
           FG GS  CPG++LA+LEI+I LHHF+L Y+++  N E  +  +P  RP +NCL +I
Sbjct: 441 FGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of IVF0024089 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 215.7 bits (548), Expect = 8.3e-56
Identity = 142/451 (31.49%), Postives = 217/451 (48.12%), Query Frame = 0


Query: 41  LPPGDLGWPVIGSSFSSYKTFMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSE 100
           LPPG +GWP +G +   Y      ++P  F  S   RYGE  I+KT + G P V+    E
Sbjct: 34  LPPGSMGWPYLGETLQLY-----SQNPNVFFTSKQKRYGE--IFKTRILGYPCVMLASPE 93

Query: 101 ICRRIYLDEA-KFKQHNPESVKILEGSSG-DFSKMDHKIAYKVVATPMNGSEVLSNYVDF 160
             R + +  A  FK   P S + L G S   F + D+    + +       E +   +  
Sbjct: 94  AARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYPETIRKLIPD 153

Query: 161 IEEVIAKGLEEWSSMLREPIKLLDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKEL-- 220
           IE +    L+ W++M    +    E+    F V      G          L + YKE+  
Sbjct: 154 IEHIALSSLQSWANM--PIVSTYQEMKKFAFDVGILAIFG---------HLESSYKEILK 213

Query: 221 -SFAMV---MSTFPYDFPGFTFHQALKARKKIENIIQGVVEEKRRRFENDKTSEVQCQVD 280
            ++ +V    ++FP   PG ++H+AL ARK+++ I+  ++ E+R +        +Q    
Sbjct: 214 HNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREK------RALQTDFL 273

Query: 281 KMVVAIDENGAKLYNNNLIRDLLLGVLFAGHSTPAIAAFWALLHISQNPHVFQKAKEEQE 340
             ++       ++     I D ++GVLFA   T A    W L ++  +  + +  K EQ+
Sbjct: 274 GHLLNFKNEKGRVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQK 333

Query: 341 SIIRQRPSNQKGLTLNEIKQMKYLTKVINEVLRRNTITPTNFREAKTDVNINGYFIPKGW 400
           +I  +    +K LT  + + M    KVI E LR  +I    FREA  DV   GY IPKGW
Sbjct: 334 AIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGW 393

Query: 401 TIQIWNIAVHMDPQIHSNPQEFNPSRWENYTPKPGTFIPFGLGSRFCPGSELARLEITIL 460
            +      +H +P+  SNP+ F+PSR+E   PKP TF+PFG G   CPG+ELA+L+I I 
Sbjct: 394 KVMPLFRNIHHNPKYFSNPEVFDPSRFE-VNPKPNTFMPFGSGVHACPGNELAKLQILIF 453

Query: 461 LHHFILNYKMEGVNLESYITQMPSPRPENCL 484
           LHH + N++ E    E  I   P P P+N L
Sbjct: 454 LHHLVSNFRWEVKGGEKGIQYSPFPIPQNGL 459

[Close]

BLAST of IVF0024089 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 209.5 bits (532), Expect = 6.0e-54
Identity = 135/459 (29.41%), Postives = 234/459 (50.98%), Query Frame = 0


Query: 12  VVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKTFMVQEDPISFI 71
           +++ VSLL+    +  W       K    LPPG +G+P++G S   +K     + P  FI
Sbjct: 4   LLIWVSLLLISITH--WVYSWRNPKCRGKLPPGSMGFPLLGESIQFFKPNKTSDIP-PFI 63

Query: 72  QSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAK-FKQHNPESVKILEGSS--G 131
           +    +YG   I+KT+L G+P++++TD+++   ++  E + F+   P++   + G    G
Sbjct: 64  KERVKKYGP--IFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVG 123

Query: 132 DFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKLLDEIGSLF 191
                 +K    +V T + G + L   +  +E    K LE WS+  ++ ++L D   S+ 
Sbjct: 124 SLHGFMYKYLKNMVLT-LFGHDGLKKMLPQVEMTANKRLELWSN--QDSVELKDATASMI 183

Query: 192 FKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKARKKIENII 251
           F +  K  +    D K+   L A +  ++F   + +FP+D PG  +H+ L+ R K   ++
Sbjct: 184 FDLTAKKLISHDPD-KSSENLRANF--VAFIQGLISFPFDIPGTAYHKCLQGRAKAMKML 243

Query: 252 QGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLFAGHSTPAI 311
           + +++E+R   EN + +      D ++  I + G  +    +  DL+  +LFA   T ++
Sbjct: 244 RNMLQERR---ENPRKNPSDF-FDYVIEEIQKEGT-ILTEEIALDLMFVLLFASFETTSL 303

Query: 312 AAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVINEVLRRNT 371
           A   A+  +S +P V ++  EE E+I+R R     GLT  E K M Y  + INE  R   
Sbjct: 304 ALTLAIKFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLAN 363

Query: 372 ITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE--NYTPKP 431
           I P  FR+A  D+    Y IP GW + +   AVH++P+++ +P  FNPSRWE    T   
Sbjct: 364 IVPAIFRKALRDIKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNAS 423

Query: 432 GTFIPFGLGSRFCPGSELARLEITILLHHFILNYKMEGV 466
             F+ FG G RFC G++  +L++   LH  +  Y+ E +
Sbjct: 424 KHFMAFGGGMRFCVGTDFTKLQMAAFLHSLVTKYRWEEI 446

The following BLAST results are available for this feature:

BLAST of IVF0024089 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	3.5e-115	44.38	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	1.4e-103	43.98	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q5VRM7	3.5e-99	37.40	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q9AXH9	5.5e-97	37.37	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]
O23051	3.6e-96	40.55	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]

BLAST of IVF0024089 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CRX8	4.2e-265	100.00	beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103504002 PE=3 SV=1	[more]
A0A097IYP6	1.8e-255	89.39	Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1	[more]
A0A6J1GLR1	3.9e-186	67.14	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 S...	[more]
A0A6J1GJM1	5.1e-186	65.92	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S...	[more]
A0A5D3E6B2	1.8e-183	100.00	Beta-amyrin 11-oxidase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]

BLAST of IVF0024089 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008466645.1	0.0	100.00	PREDICTED: beta-amyrin 11-oxidase-like, partial [Cucumis melo]	[more]
XP_004148515.2	0.0	89.39	beta-amyrin 11-oxidase [Cucumis sativus] >AIT72036.1 cytochrome P450 [Cucumis sa...	[more]
XP_011652660.1	3.11e-247	68.76	beta-amyrin 11-oxidase [Cucumis sativus]	[more]
KAE8651450.1	4.16e-233	69.28	hypothetical protein Csa_001467 [Cucumis sativus]	[more]
XP_022952843.1	1.07e-232	67.14	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]

BLAST of IVF0024089 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	9.7e-105	43.98	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	9.7e-105	43.98	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	2.6e-97	40.55	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT3G19270.1	8.3e-56	31.49	cytochrome P450, family 707, subfamily A, polypeptide 4	[more]
AT1G12740.1	6.0e-54	29.41	cytochrome P450, family 87, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 301..318 score: 27.65 coord: 358..369 score: 36.04 coord: 430..439 score: 56.28 coord: 439..450 score: 41.8
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 42..465 e-value: 1.8E-72 score: 244.6
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 310..336 score: 25.71 coord: 439..462 score: 27.88 coord: 357..375 score: 29.16 coord: 187..205 score: 20.38 coord: 429..439 score: 54.21 coord: 77..96 score: 20.33 coord: 397..421 score: 27.65
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 35..487 e-value: 1.9E-92 score: 312.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 39..466
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 9..471 e-value: 2.0E-22 score: 77.6
None	No IPR available	PANTHER	PTHR24286:SF289	ENT-KAURENOIC ACID OXIDASE 2	coord: 15..475
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 15..475
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 432..441

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0024089.1	IVF0024089.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0051777	ent-kaurenoate oxidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen