IVF0023874 (gene) Melon (IVF77) v1

Overview
NameIVF0023874
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionGRAS domain-containing protein
Locationchr03: 12412243 .. 12413688 (+)
RNA-Seq ExpressionIVF0023874
SyntenyIVF0023874
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTTGGCCATTAACAACCCTTTTAATGATATCGCTCTCTCCAATTACAGCACCTCCACCAACAACTCCGACGACCACCGCCACCTCGCCGGTAACTGGAATTATAGCTCTCCAATTGTCGACTGGGAAACCTTTTCCGGCACCCACAACGACTTTCTCGACGTCTTCGACTCCACGATATGCATCGAAAATCCACCATTTCTGACGGAGGATCGAGATGAAGAGGATGAATTTAAAGGCTTAAGGCTTTATCACCTCCTCATAGCGGCAGCCGACGCCGTGTTCGGCGACCATAGGAGTTGCGATTTGGCTCATGTCATATTGGTTCGGCTCAATGAATTGGTTTCCCCTTCACATGGGACAAATCTTGAACGCCTCACTGCGTATTACGCCCAAGCTTTTCAGGATTTGCTCGATTCAGCACCAGTATCGAATAAAACCCATCATCACAACCACCATATCCACCACCGTGATGATCATACTCCAACGGACGTTCTTGCAGCGTTTCAGTTGCTGCAGGAAATGTCCCCTTATGTGAAATTCGGCCATTTCACTGCAAATCAAGCGATTCTTGAGGCTGTTGCAGAGGATCGAAGAGTTCACATTGTGGATTATGATATTATGGAAGGGATTCAATGGGCGTCGTTAATGCAAGCTTTCGTCTCGAGCCCATCGGCTCCACATCTCAGAATCACTGCTATTTCTAGAGGAGCTAACGGGCGGAGATCGATTGGGACGGTTCAAGAAACAGGGCGGCGATTAGTGGCGTTTGCAGCTTCAATTGGTCAACCCTTTTCGTTTCATCAATGTAAATTGGATTCTGATGAAAGTTTTCGTCCTTCTGGATTGAAATTGGTAAAAGGTGAAGCGCTTGTTGTGAATTGTATGCTTCATCTTCCTCATTTTAGTTACCGGTCACCGGAATCTATTGCATCTTTTCTCTCCGGCGCCAAGAGTTTGAGTCCGAGGATTGTGACTTTGGTGGAAGAGGAAATAGGTCATGGACCCACCATTGACGGCGATTACAAGGTTCAATTTCTTGATTCATTAGAGCGTTACTCGGCGATATATGACTCACTGGAAGCGGTGATTCCGATGAAAAACAGAGCAAGAGCATTGGTGGAGAGGGTTTTTCTCGGGCCAAGGATCTCAGCGACTCTGAGGCGAATCGGACAGCGTAGGTGGTCAACGGCGGCAATGGAGGAGAATTGCTTGTGGGGAGAGAAATTGGAGAAGATGGGATTGAAGACAGTGAAGATTAGCTTTGCGAATCATTGCCAAGCGAGATTGTTGTTGGGTTTGTTCAATGACGGGTACAGAGTTGAGGAATTGGGCAATAATAAGCTTGTTTTGGGATGGAAATCGAAGCGTTTGCTTTCGGTTTCAATTTGGGGTTCTTCAACTTCTTCATCTTCTTCGTCTTCTTTATCTGATTCTGAGTAG

mRNA sequence

ATGGCCTTGGCCATTAACAACCCTTTTAATGATATCGCTCTCTCCAATTACAGCACCTCCACCAACAACTCCGACGACCACCGCCACCTCGCCGGTAACTGGAATTATAGCTCTCCAATTGTCGACTGGGAAACCTTTTCCGGCACCCACAACGACTTTCTCGACGTCTTCGACTCCACGATATGCATCGAAAATCCACCATTTCTGACGGAGGATCGAGATGAAGAGGATGAATTTAAAGGCTTAAGGCTTTATCACCTCCTCATAGCGGCAGCCGACGCCGTGTTCGGCGACCATAGGAGTTGCGATTTGGCTCATGTCATATTGGTTCGGCTCAATGAATTGGTTTCCCCTTCACATGGGACAAATCTTGAACGCCTCACTGCGTATTACGCCCAAGCTTTTCAGGATTTGCTCGATTCAGCACCAGTATCGAATAAAACCCATCATCACAACCACCATATCCACCACCGTGATGATCATACTCCAACGGACGTTCTTGCAGCGTTTCAGTTGCTGCAGGAAATGTCCCCTTATGTGAAATTCGGCCATTTCACTGCAAATCAAGCGATTCTTGAGGCTGTTGCAGAGGATCGAAGAGTTCACATTGTGGATTATGATATTATGGAAGGGATTCAATGGGCGTCGTTAATGCAAGCTTTCGTCTCGAGCCCATCGGCTCCACATCTCAGAATCACTGCTATTTCTAGAGGAGCTAACGGGCGGAGATCGATTGGGACGGTTCAAGAAACAGGGCGGCGATTAGTGGCGTTTGCAGCTTCAATTGGTCAACCCTTTTCGTTTCATCAATGTAAATTGGATTCTGATGAAAGTTTTCGTCCTTCTGGATTGAAATTGGTAAAAGGTGAAGCGCTTGTTGTGAATTGTATGCTTCATCTTCCTCATTTTAGTTACCGGTCACCGGAATCTATTGCATCTTTTCTCTCCGGCGCCAAGAGTTTGAGTCCGAGGATTGTGACTTTGGTGGAAGAGGAAATAGGTCATGGACCCACCATTGACGGCGATTACAAGGTTCAATTTCTTGATTCATTAGAGCGTTACTCGGCGATATATGACTCACTGGAAGCGGTGATTCCGATGAAAAACAGAGCAAGAGCATTGGTGGAGAGGGTTTTTCTCGGGCCAAGGATCTCAGCGACTCTGAGGCGAATCGGACAGCGTAGGTGGTCAACGGCGGCAATGGAGGAGAATTGCTTGTGGGGAGAGAAATTGGAGAAGATGGGATTGAAGACAGTGAAGATTAGCTTTGCGAATCATTGCCAAGCGAGATTGTTGTTGGGTTTGTTCAATGACGGGTACAGAGTTGAGGAATTGGGCAATAATAAGCTTGTTTTGGGATGGAAATCGAAGCGTTTGCTTTCGGTTTCAATTTGGGGTTCTTCAACTTCTTCATCTTCTTCGTCTTCTTTATCTGATTCTGAGTAG

Coding sequence (CDS)

ATGGCCTTGGCCATTAACAACCCTTTTAATGATATCGCTCTCTCCAATTACAGCACCTCCACCAACAACTCCGACGACCACCGCCACCTCGCCGGTAACTGGAATTATAGCTCTCCAATTGTCGACTGGGAAACCTTTTCCGGCACCCACAACGACTTTCTCGACGTCTTCGACTCCACGATATGCATCGAAAATCCACCATTTCTGACGGAGGATCGAGATGAAGAGGATGAATTTAAAGGCTTAAGGCTTTATCACCTCCTCATAGCGGCAGCCGACGCCGTGTTCGGCGACCATAGGAGTTGCGATTTGGCTCATGTCATATTGGTTCGGCTCAATGAATTGGTTTCCCCTTCACATGGGACAAATCTTGAACGCCTCACTGCGTATTACGCCCAAGCTTTTCAGGATTTGCTCGATTCAGCACCAGTATCGAATAAAACCCATCATCACAACCACCATATCCACCACCGTGATGATCATACTCCAACGGACGTTCTTGCAGCGTTTCAGTTGCTGCAGGAAATGTCCCCTTATGTGAAATTCGGCCATTTCACTGCAAATCAAGCGATTCTTGAGGCTGTTGCAGAGGATCGAAGAGTTCACATTGTGGATTATGATATTATGGAAGGGATTCAATGGGCGTCGTTAATGCAAGCTTTCGTCTCGAGCCCATCGGCTCCACATCTCAGAATCACTGCTATTTCTAGAGGAGCTAACGGGCGGAGATCGATTGGGACGGTTCAAGAAACAGGGCGGCGATTAGTGGCGTTTGCAGCTTCAATTGGTCAACCCTTTTCGTTTCATCAATGTAAATTGGATTCTGATGAAAGTTTTCGTCCTTCTGGATTGAAATTGGTAAAAGGTGAAGCGCTTGTTGTGAATTGTATGCTTCATCTTCCTCATTTTAGTTACCGGTCACCGGAATCTATTGCATCTTTTCTCTCCGGCGCCAAGAGTTTGAGTCCGAGGATTGTGACTTTGGTGGAAGAGGAAATAGGTCATGGACCCACCATTGACGGCGATTACAAGGTTCAATTTCTTGATTCATTAGAGCGTTACTCGGCGATATATGACTCACTGGAAGCGGTGATTCCGATGAAAAACAGAGCAAGAGCATTGGTGGAGAGGGTTTTTCTCGGGCCAAGGATCTCAGCGACTCTGAGGCGAATCGGACAGCGTAGGTGGTCAACGGCGGCAATGGAGGAGAATTGCTTGTGGGGAGAGAAATTGGAGAAGATGGGATTGAAGACAGTGAAGATTAGCTTTGCGAATCATTGCCAAGCGAGATTGTTGTTGGGTTTGTTCAATGACGGGTACAGAGTTGAGGAATTGGGCAATAATAAGCTTGTTTTGGGATGGAAATCGAAGCGTTTGCTTTCGGTTTCAATTTGGGGTTCTTCAACTTCTTCATCTTCTTCGTCTTCTTTATCTGATTCTGAGTAG

Protein sequence

MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDSTICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDSE
Homology
BLAST of IVF0023874 vs. ExPASy Swiss-Prot
Match: Q5NE24 (Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 6.3e-146
Identity = 279/513 (54.39%), Postives = 360/513 (70.18%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFD-- 60
           M L   +  ND+  S +S+ TN           WN+ SPIV+W+TF+G  +DF  + D  
Sbjct: 1   MDLMDMDAINDLHFSGHSSLTNTPTSDEDYGCTWNHWSPIVNWDTFTGAPDDFHHLMDTI 60

Query: 61  ----STICIENPPFL--------TEDRDEE-------------------DEFKGLRLYHL 120
               +T+  +  P +        T D +EE                   D+ KGL+L HL
Sbjct: 61  IEDRTTVLEQLSPSITTTTTTTTTTDEEEEEMETTTTTTTTAIKTHEVGDDSKGLKLVHL 120

Query: 121 LIAAADAVFGDHRSCDLAHVILVRLNELVSP-SHGTNLERLTAYYAQAFQDLLDSA-PVS 180
           L+A A+A+ G  ++ DLA VIL+RL ELVS  ++G+N+ERL A++ +A   LL+ A    
Sbjct: 121 LMAGAEALTGSTKNRDLARVILIRLKELVSQHANGSNMERLAAHFTEALHGLLEGAGGAH 180

Query: 181 NKTHHHNHHIHHRDDHTP----TDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRV 240
           N  HHHN++ H+   + P     D LAAFQLLQ+MSPYVKFGHFTANQAI+EAVA +RRV
Sbjct: 181 NNHHHHNNNKHYLTTNGPHDNQNDTLAAFQLLQDMSPYVKFGHFTANQAIIEAVAHERRV 240

Query: 241 HIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGANGRRSIGTVQETGRRLVAFAAS 300
           H++DYDIMEG+QWASL+Q+  S+ + PHLRITA+SR   GRRSI TVQETGRRL +FAAS
Sbjct: 241 HVIDYDIMEGVQWASLIQSLASNNNGPHLRITALSRTGTGRRSIATVQETGRRLTSFAAS 300

Query: 301 IGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSL 360
           +GQPFSFH C+LDSDE+FRPS LKLV+GEALV NCML+LPH SYR+PES+ASFL+GAK+L
Sbjct: 301 LGQPFSFHHCRLDSDETFRPSALKLVRGEALVFNCMLNLPHLSYRAPESVASFLNGAKTL 360

Query: 361 SPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLG 420
           +P++VTLVEEE+G   ++ G +  +F+DSL  YSA++DSLEA  PM+NRAR LVERVF G
Sbjct: 361 NPKLVTLVEEEVG---SVIGGFVERFMDSLHHYSAVFDSLEAGFPMQNRARTLVERVFFG 420

Query: 421 PRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFNDGYR 473
           PRI+ +L RI    + T   EE   WGE L ++G + V +SFANHCQA+LLLGLFNDGYR
Sbjct: 421 PRIAGSLGRI----YRTGGEEERRSWGEWLGEVGFRGVPVSFANHCQAKLLLGLFNDGYR 480

BLAST of IVF0023874 vs. ExPASy Swiss-Prot
Match: Q2PEG7 (Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 5.6e-142
Identity = 275/490 (56.12%), Postives = 345/490 (70.41%), Query Frame = 0

Query: 15  SNYSTSTNN-SDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDSTICIEN--PPF--- 74
           S +ST TN  S D+ +   +WN+ SP+V+W+ F+G  +DF  + DS I   N  P F   
Sbjct: 14  SGHSTLTNTPSSDNDNYGCSWNHWSPVVNWDAFTGNQDDFHHLIDSMIDDNNTGPAFSDH 73

Query: 75  ----LTEDRDEE----------------------DEFKGLRLYHLLIAAADAVFGDHRSC 134
                +E+ +EE                      D+FKGLRL HLL+A A+A+ G +++ 
Sbjct: 74  TASTTSEEEEEEEATTTTMTTTTTTTTTTPEAADDDFKGLRLVHLLMAGAEALTGANKNR 133

Query: 135 DLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHT 194
           +LA VILVRL ELVS + GTN+ERL AY+ +A Q LL+ A  +  +    HH+     H 
Sbjct: 134 ELARVILVRLKELVSHTDGTNMERLAAYFTEALQGLLEGAGGAYNS-SSKHHVIGGPHHE 193

Query: 195 P-TDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAF 254
           P  D LAAFQLLQ+MSPYVKFGHFTANQAI+EAVA +RRVHIVDYDIMEG+QWASLMQA 
Sbjct: 194 PQNDALAAFQLLQDMSPYVKFGHFTANQAIVEAVAHERRVHIVDYDIMEGVQWASLMQAL 253

Query: 255 VSSPSAPHLRITAISRGANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRP 314
            S+P+ PHLRITA+SR   GRRS+ TVQETGRRL AFA S+GQPFSFH  +L+SDE+FRP
Sbjct: 254 ASNPNGPHLRITALSRSGVGRRSMATVQETGRRLTAFATSLGQPFSFHHSRLESDETFRP 313

Query: 315 SGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDG 374
           +GLKLV+GEALV NCML+LPH +YRSP S+ASFL+ AK+L PR+VT+VEEE+G   +  G
Sbjct: 314 AGLKLVRGEALVFNCMLNLPHLTYRSPNSVASFLTAAKALRPRLVTVVEEEVG---SALG 373

Query: 375 DYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAM 434
            +  +F+DSL  +SA++DSLEA  PM+ RARALVERVFLGPRI  +L RI +   +    
Sbjct: 374 GFVERFMDSLHHFSAVFDSLEAGFPMQGRARALVERVFLGPRIVGSLARIYR---TGGGG 433

Query: 435 EENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLS 472
           EE   W E L   G   V +S ANHCQ+ LLLGLFNDGYRVEELG+NKLVL WK++RLLS
Sbjct: 434 EERGSWREWLRAAGFSGVAVSSANHCQSNLLLGLFNDGYRVEELGSNKLVLHWKTRRLLS 493

BLAST of IVF0023874 vs. ExPASy Swiss-Prot
Match: Q9SUF5 (Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.2e-114
Identity = 220/396 (55.56%), Postives = 281/396 (70.96%), Query Frame = 0

Query: 77  DEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQ 136
           DE KGLRL HLL+AAADA  G ++S +L  VIL RL +LVSP   TN+ERL A++     
Sbjct: 97  DESKGLRLVHLLVAAADASTGANKSRELTRVILARLKDLVSPGDRTNMERLAAHFTNGLS 156

Query: 137 DLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVA 196
            LL+   V     H +      D +   DV++AF+LLQ MSPYV FG+ TA QAILEAV 
Sbjct: 157 KLLERDSVLCPQQHRD------DVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVK 216

Query: 197 EDRRVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISRGANGRRSIGTVQETGR 256
            +RR+HIVDYDI EG+QWASLMQA VS    PSA HLRITA+SR  NG++S+  VQETGR
Sbjct: 217 YERRIHIVDYDINEGVQWASLMQALVSRNTGPSAQHLRITALSRATNGKKSVAAVQETGR 276

Query: 257 RLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIAS 316
           RL AFA SIGQPFS+  CKLD++ +F  S LKLV+GEA+V+NCMLHLP FS+++P S+ S
Sbjct: 277 RLTAFADSIGQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSHQTPSSVIS 336

Query: 317 FLSGAKSLSPRIVTLVEEEIGHGPTIDGD--YKVQFLDSLERYSAIYDSLEAVIPMKNRA 376
           FLS AK+L+P++VTLV EE+G    + G+  +  +F+D L ++SAI+DSLEA + + N A
Sbjct: 337 FLSEAKTLNPKLVTLVHEEVG----LMGNQGFLYRFMDLLHQFSAIFDSLEAGLSIANPA 396

Query: 377 RALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARL 436
           R  VERVF+GP ++  L RI     + A +E    W + LE  G K +++SF N CQA+L
Sbjct: 397 RGFVERVFIGPWVANWLTRITA---NDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKL 456

Query: 437 LLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGS 468
           LL LFNDG+RVEELG N LVLGWKS+RL+S S W S
Sbjct: 457 LLSLFNDGFRVEELGQNGLVLGWKSRRLVSASFWAS 478

BLAST of IVF0023874 vs. ExPASy Swiss-Prot
Match: Q84Q92 (Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 GN=NSP2 PE=1 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 9.9e-107
Identity = 224/408 (54.90%), Postives = 271/408 (66.42%), Query Frame = 0

Query: 80  KGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVS-----PSHGTNLERLTAYYAQA 139
           KGLRL HLL+AAA+A+ G H+S +LA VILVRL E+VS      +  +N+ERL A++  A
Sbjct: 128 KGLRLLHLLMAAAEALSGPHKSRELARVILVRLKEMVSHTASANAAASNMERLAAHFTDA 187

Query: 140 FQDLLD-SAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILE 199
            Q LLD S PV            H   H   DVL AFQ+LQ+MSPY+KFGHFTANQAILE
Sbjct: 188 LQGLLDGSHPVGGSGRQAAAAASH---HHAGDVLTAFQMLQDMSPYMKFGHFTANQAILE 247

Query: 200 AVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPS---APHLRITAISR-GANGRRSIGTVQ 259
           AV+ DRRVHIVDYDI EGIQWASLMQA  S      APHLRITA+SR G  G R+   VQ
Sbjct: 248 AVSGDRRVHIVDYDIAEGIQWASLMQAMTSRADGVPAPHLRITAVSRSGGGGARA---VQ 307

Query: 260 ETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFS---YR 319
           E GRRL AFAASIGQPFSF QC+LDSDE FRP+ +++VKGEALV NC+LH    +    R
Sbjct: 308 EAGRRLSAFAASIGQPFSFGQCRLDSDERFRPATVRMVKGEALVANCVLHQAAATTTIRR 367

Query: 320 SPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGD---------YKVQFLDSLERYSAI 379
              S+ASFLSG  +L  ++VT+VEEE       DGD         +  QF++ L RYSA+
Sbjct: 368 PTGSVASFLSGMAALGAKLVTVVEEEGEAEKDDDGDSAGDAAAGGFVRQFMEELHRYSAV 427

Query: 380 YDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLK 439
           +DSLEA  P ++R R LVERV L P I+  +     R +     E  C WG+ +   G  
Sbjct: 428 WDSLEAGFPTQSRVRGLVERVILAPNIAGAV----SRAYRGVDGEGRCGWGQWMRGSGFT 487

Query: 440 TVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIW 466
            V +S  NH QARLLLGLFNDGY VEE G NK+VLGWK++RL+S S+W
Sbjct: 488 AVPLSCFNHSQARLLLGLFNDGYTVEETGPNKIVLGWKARRLMSASVW 525

BLAST of IVF0023874 vs. ExPASy Swiss-Prot
Match: G7L166 (GRAS family protein RAM1 OS=Medicago truncatula OX=3880 GN=RAM1 PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.2e-50
Identity = 144/408 (35.29%), Postives = 219/408 (53.68%), Query Frame = 0

Query: 75  EEDEFKGLRLYHLLIAAADAVF-GDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQ 134
           E+++  GL+L HLL+A A+AV  G++    LA   L +LN +V+P  G +++R+ + + +
Sbjct: 288 EQEQDSGLQLVHLLLACAEAVAKGEYM---LARRYLHQLNRVVTPL-GDSMQRVASCFTE 347

Query: 135 AFQDLLDS------------APVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVK 194
           +    L +            AP S  +   +  +     + P +VL  +Q++ +  PY+K
Sbjct: 348 SLSARLAATLTTKSSSTKKLAPSSLSSSSSSSCLSTFPSN-PMEVLKIYQIVYQACPYIK 407

Query: 195 FGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSP-SAPHLRITAISRGAN 254
           F HFTANQAI EA   + RVH++D DI++G QW + MQA  + P  AP LRIT +     
Sbjct: 408 FAHFTANQAIFEAFEAEERVHVIDLDILQGYQWPAFMQALAARPGGAPFLRITGVG---- 467

Query: 255 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 314
               I +V+ETGR L   A S+  PF FH    +  E  +P       GEAL VN +  L
Sbjct: 468 --PCIESVRETGRCLTELAHSLRIPFEFHPVG-EQLEDLKPHMFNRRVGEALAVNTVNRL 527

Query: 315 PHFSYRSP-ESIASFLSGAKSLSPRIVTLVEEEIGH-GPTIDGDYKVQFLDSLERYSAIY 374
               +R P   + + LS  +  +P IVTLVE+E  H GP   G    +FL++L  YSAI+
Sbjct: 528 ----HRVPGNHLGNLLSMIRDQAPNIVTLVEQEASHNGPYFLG----RFLEALHYYSAIF 587

Query: 375 DSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKT 434
           DSL+A  P+++  RA VE+    P I   +   G+ R       E   W + +E  G K 
Sbjct: 588 DSLDATFPVESAPRAKVEQYIFAPEIRNIVACEGEERIERHERLEK--WRKIMEGKGFKG 647

Query: 435 VKISFANHCQARLLLGLFN-DGYRVEELGNNKLVLGWKSKRLLSVSIW 466
           V +S     Q+R+LLGL++ DGYR+ E     L+LGW+ + +++ S W
Sbjct: 648 VPLSPNAVTQSRILLGLYSCDGYRLTE-DKGCLLLGWQDRAIIAASAW 672

BLAST of IVF0023874 vs. ExPASy TrEMBL
Match: A0A5A7V767 (Nodulation-signaling pathway 2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1415G00580 PE=3 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 4.7e-277
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60
           MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH 120
           ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH
Sbjct: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH 120

Query: 121 GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV 180
           GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV
Sbjct: 121 GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV 180

Query: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240
           KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN
Sbjct: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240

Query: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300
           GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL
Sbjct: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300

Query: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360
           PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS
Sbjct: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360

Query: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK 420
           LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK
Sbjct: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK 420

Query: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS 480
           ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS
Sbjct: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS 480

Query: 481 E 482
           E
Sbjct: 481 E 481

BLAST of IVF0023874 vs. ExPASy TrEMBL
Match: A0A1S3BE54 (nodulation-signaling pathway 2 protein OS=Cucumis melo OX=3656 GN=LOC103488636 PE=3 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 4.7e-277
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60
           MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH 120
           ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH
Sbjct: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH 120

Query: 121 GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV 180
           GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV
Sbjct: 121 GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV 180

Query: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240
           KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN
Sbjct: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240

Query: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300
           GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL
Sbjct: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300

Query: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360
           PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS
Sbjct: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360

Query: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK 420
           LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK
Sbjct: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK 420

Query: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS 480
           ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS
Sbjct: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS 480

Query: 481 E 482
           E
Sbjct: 481 E 481

BLAST of IVF0023874 vs. ExPASy TrEMBL
Match: A0A0A0KDX4 (GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040600 PE=3 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 3.4e-267
Identity = 467/481 (97.09%), Postives = 470/481 (97.71%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60
           MALAINNP  DIALSNYSTSTNNSDDH HLAGNWNY SPIVDWETFSGTHNDFLDVFDST
Sbjct: 1   MALAINNPPKDIALSNYSTSTNNSDDHCHLAGNWNYGSPIVDWETFSGTHNDFLDVFDST 60

Query: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH 120
           ICIENPPFLTEDRDEEDEFKGLRLYHLL AAADAV GDH+SCDLAHVILVRLNELVSPSH
Sbjct: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLTAAADAVLGDHKSCDLAHVILVRLNELVSPSH 120

Query: 121 GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV 180
           GTNLERLTAYYAQAFQDLLDSAPV+NKTHHHNHHIH RDDHTPTDVLAAFQLLQEMSPYV
Sbjct: 121 GTNLERLTAYYAQAFQDLLDSAPVANKTHHHNHHIHQRDDHTPTDVLAAFQLLQEMSPYV 180

Query: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240
           KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN
Sbjct: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240

Query: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300
           GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL
Sbjct: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300

Query: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360
           PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS
Sbjct: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360

Query: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK 420
           LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTA ME+NCLWGEKLEKMGLKTVK
Sbjct: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAEMEDNCLWGEKLEKMGLKTVK 420

Query: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS 480
           ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWG   SSSSSSSLSDS
Sbjct: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWG---SSSSSSSLSDS 478

Query: 481 E 482
           E
Sbjct: 481 E 478

BLAST of IVF0023874 vs. ExPASy TrEMBL
Match: A0A6J1JUC4 (nodulation-signaling pathway 2 protein OS=Cucurbita maxima OX=3661 GN=LOC111489805 PE=3 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 4.8e-197
Identity = 375/485 (77.32%), Postives = 414/485 (85.36%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60
           MA+A++N FN +A+SNYSTSTNNSDD  H   +   S   +D   +S      ++V   T
Sbjct: 1   MAMAVDNAFNGVAVSNYSTSTNNSDDDFH---DLFDSIMSIDALPYS---PQVVEVDGGT 60

Query: 61  ICIENPPFLTEDR-DEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPS 120
            C  +P    EDR +EE+EFK LRLYHLL+A ADA+FGD++S +LA VIL+RLNELVS S
Sbjct: 61  NC-NSPSNPAEDREEEEEEFKSLRLYHLLMAVADAMFGDNKSRELAQVILIRLNELVSTS 120

Query: 121 HGTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPY 180
           HGTNLERLTAYYAQAFQDLLD A VS    +  HH+ HRDDH+PTDVLAAFQLLQ+MSPY
Sbjct: 121 HGTNLERLTAYYAQAFQDLLDCAAVSGGVPNKPHHL-HRDDHSPTDVLAAFQLLQDMSPY 180

Query: 181 VKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAIS 240
           VKFGHFTANQAILEAVA+DRRVHIVDY+IMEGIQWASLMQAFVS   SP APHLRIT IS
Sbjct: 181 VKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQWASLMQAFVSRKDSPPAPHLRITVIS 240

Query: 241 RGANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNC 300
           RG + RR IGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNC
Sbjct: 241 RGPSSRRWIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC 300

Query: 301 MLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSA 360
           +LHLPHFS  SPESIASFL+GAK+L+PR+VTLVEEEI HGPTIDGDYKVQFLDSLERYSA
Sbjct: 301 VLHLPHFSNSSPESIASFLAGAKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSA 360

Query: 361 IYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGL 420
           IYDSLEA IPMKNRAR LVE+VFLGPRISATLRRIGQ + +  A EENCLWGE+LEKMGL
Sbjct: 361 IYDSLEAGIPMKNRARGLVEKVFLGPRISATLRRIGQPQRAATAAEENCLWGERLEKMGL 420

Query: 421 KTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSS 480
           K   ISFANHCQARLL+ LFNDGYRVEELG+NKLVLGWKSKRLLSVSIW SS+SSSSSSS
Sbjct: 421 KAAAISFANHCQARLLVDLFNDGYRVEELGSNKLVLGWKSKRLLSVSIWASSSSSSSSSS 476

Query: 481 LSDSE 482
            SDSE
Sbjct: 481 -SDSE 476

BLAST of IVF0023874 vs. ExPASy TrEMBL
Match: A0A6J1FK48 (nodulation-signaling pathway 2 protein OS=Cucurbita moschata OX=3662 GN=LOC111444761 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 6.5e-194
Identity = 368/503 (73.16%), Postives = 408/503 (81.11%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60
           MA+A++N FN +A+S+YSTSTNNS+D                         DF D+FDS 
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTNNSND-------------------------DFHDLFDSI 60

Query: 61  ICIENPPFLTE-------------------DRDEEDEFKGLRLYHLLIAAADAVFGDHRS 120
           + I+  P+  +                     +EE+EF+ LRLYHLL+A ADA+FGDH+S
Sbjct: 61  MSIDALPYSPQVVEIDGGTNCNSPSNPAEGREEEEEEFRSLRLYHLLMAVADAMFGDHKS 120

Query: 121 CDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDH 180
            +LA VIL+RLNELVS SHGTNLER+TAYYAQAFQDLLD A VS    +  HH+ HRDDH
Sbjct: 121 RELAQVILIRLNELVSTSHGTNLERITAYYAQAFQDLLDCAAVSGGGPNKPHHL-HRDDH 180

Query: 181 TPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAF 240
           +PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVA+DRRVHIVDY+IMEGIQWASLMQA 
Sbjct: 181 SPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQWASLMQAL 240

Query: 241 VS---SPSAPHLRITAISRGANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDES 300
           VS   SP APHLRITAISRG + RR IGTVQETGRRLVAFAASIGQPFSFHQCKLDSDES
Sbjct: 241 VSRKDSPPAPHLRITAISRGPSSRRWIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDES 300

Query: 301 FRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPT 360
           FRPSGLKLV+GEALVVNC+LHLPHFS  SPESIASFL+GAK+L+PR+VTLVEEEI HGPT
Sbjct: 301 FRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLVEEEIEHGPT 360

Query: 361 IDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWST 420
           IDGDYKVQFLDSLERYSAIYDSLEA IPMKNRAR LVE+VFLGPRISATLRRI Q + + 
Sbjct: 361 IDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLRRIEQPQRAA 420

Query: 421 AAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKR 480
            A EENCLWGE+LEKMGLK   ISFANHCQARLL+ LFNDGYRVEELG+NKLVLGWKSKR
Sbjct: 421 TAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNKLVLGWKSKR 476

Query: 481 LLSVSIWGSSTSSSSSSSLSDSE 482
           LLSVSIW SS+ SSSSSS SDSE
Sbjct: 481 LLSVSIWASSSLSSSSSS-SDSE 476

BLAST of IVF0023874 vs. NCBI nr
Match: XP_008445682.1 (PREDICTED: nodulation-signaling pathway 2 protein [Cucumis melo] >KAA0063953.1 nodulation-signaling pathway 2 protein [Cucumis melo var. makuwa] >TYK05186.1 nodulation-signaling pathway 2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60
           MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST
Sbjct: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60

Query: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH 120
           ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH
Sbjct: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH 120

Query: 121 GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV 180
           GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV
Sbjct: 121 GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV 180

Query: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240
           KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN
Sbjct: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240

Query: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300
           GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL
Sbjct: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300

Query: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360
           PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS
Sbjct: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360

Query: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK 420
           LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK
Sbjct: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK 420

Query: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS 480
           ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS
Sbjct: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS 480

BLAST of IVF0023874 vs. NCBI nr
Match: XP_004140976.1 (protein NODULATION SIGNALING PATHWAY 2 [Cucumis sativus] >KGN45971.1 hypothetical protein Csa_004902 [Cucumis sativus])

HSP 1 Score: 925 bits (2391), Expect = 0.0
Identity = 466/481 (96.88%), Postives = 470/481 (97.71%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60
           MALAINNP  DIALSNYSTSTNNSDDH HLAGNWNY SPIVDWETFSGTHNDFLDVFDST
Sbjct: 1   MALAINNPPKDIALSNYSTSTNNSDDHCHLAGNWNYGSPIVDWETFSGTHNDFLDVFDST 60

Query: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH 120
           ICIENPPFLTEDRDEEDEFKGLRLYHLL AAADAV GDH+SCDLAHVILVRLNELVSPSH
Sbjct: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLTAAADAVLGDHKSCDLAHVILVRLNELVSPSH 120

Query: 121 GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV 180
           GTNLERLTAYYAQAFQDLLDSAPV+NKTHHHNHHIH RDDHTPTDVLAAFQLLQEMSPYV
Sbjct: 121 GTNLERLTAYYAQAFQDLLDSAPVANKTHHHNHHIHQRDDHTPTDVLAAFQLLQEMSPYV 180

Query: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240
           KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN
Sbjct: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240

Query: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300
           GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL
Sbjct: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300

Query: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360
           PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS
Sbjct: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360

Query: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK 420
           LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTA ME+NCLWGEKLEKMGLKTVK
Sbjct: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAEMEDNCLWGEKLEKMGLKTVK 420

Query: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS 480
           ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSS+SSSS   LSDS
Sbjct: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSSSSSS---LSDS 478

BLAST of IVF0023874 vs. NCBI nr
Match: XP_038884439.1 (protein NODULATION SIGNALING PATHWAY 2 [Benincasa hispida])

HSP 1 Score: 873 bits (2256), Expect = 0.0
Identity = 440/481 (91.48%), Postives = 456/481 (94.80%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60
           MALA++N FN+I LSNYSTSTNNSDDHRHLAGNWNY SPIVDWETF  THNDF D+ DS 
Sbjct: 23  MALAMDNLFNEITLSNYSTSTNNSDDHRHLAGNWNYGSPIVDWETFPDTHNDFHDLVDSM 82

Query: 61  ICIENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSH 120
           ICI+NPP  TEDR+EE++FKGLRLYHLL+AAADAVFGDH+S DLAHVILVRLNELVSPSH
Sbjct: 83  ICIDNPPSPTEDREEEEDFKGLRLYHLLVAAADAVFGDHKSRDLAHVILVRLNELVSPSH 142

Query: 121 GTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYV 180
           GTNL+RLTAYYAQAFQDLLDS PVSNKTHHHNHH+H RDDHTPTDVLAAFQLLQEMSPYV
Sbjct: 143 GTNLQRLTAYYAQAFQDLLDSTPVSNKTHHHNHHLH-RDDHTPTDVLAAFQLLQEMSPYV 202

Query: 181 KFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 240
           KF HFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN
Sbjct: 203 KFSHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSAPHLRITAISRGAN 262

Query: 241 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHL 300
           GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNC+LHL
Sbjct: 263 GRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCILHL 322

Query: 301 PHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDS 360
           PHFSYRSPESI SFLSG KSL+PRIVTLVEEEIGHGPTID DYKVQFLDSLERYSAIYDS
Sbjct: 323 PHFSYRSPESIGSFLSGTKSLNPRIVTLVEEEIGHGPTIDVDYKVQFLDSLERYSAIYDS 382

Query: 361 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVK 420
           LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRW+TA  EENCLWGEKLEKMGLK   
Sbjct: 383 LEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWATAGAEENCLWGEKLEKMGLKPAT 442

Query: 421 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSSLSDS 480
           ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLS SIW SS+ SSSSSSLSDS
Sbjct: 443 ISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSASIWVSSSLSSSSSSLSDS 502

BLAST of IVF0023874 vs. NCBI nr
Match: XP_022993952.1 (nodulation-signaling pathway 2 protein [Cucurbita maxima])

HSP 1 Score: 693 bits (1788), Expect = 3.76e-247
Identity = 375/485 (77.32%), Postives = 414/485 (85.36%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60
           MA+A++N FN +A+SNYSTSTNNSDD  H   +   S   +D   +S      ++V   T
Sbjct: 1   MAMAVDNAFNGVAVSNYSTSTNNSDDDFHDLFD---SIMSIDALPYSPQ---VVEVDGGT 60

Query: 61  ICIENPPFLTEDRDEEDE-FKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPS 120
            C  +P    EDR+EE+E FK LRLYHLL+A ADA+FGD++S +LA VIL+RLNELVS S
Sbjct: 61  NC-NSPSNPAEDREEEEEEFKSLRLYHLLMAVADAMFGDNKSRELAQVILIRLNELVSTS 120

Query: 121 HGTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPY 180
           HGTNLERLTAYYAQAFQDLLD A VS    +  HH+H RDDH+PTDVLAAFQLLQ+MSPY
Sbjct: 121 HGTNLERLTAYYAQAFQDLLDCAAVSGGVPNKPHHLH-RDDHSPTDVLAAFQLLQDMSPY 180

Query: 181 VKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAIS 240
           VKFGHFTANQAILEAVA+DRRVHIVDY+IMEGIQWASLMQAFVS   SP APHLRIT IS
Sbjct: 181 VKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQWASLMQAFVSRKDSPPAPHLRITVIS 240

Query: 241 RGANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNC 300
           RG + RR IGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLV+GEALVVNC
Sbjct: 241 RGPSSRRWIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVRGEALVVNC 300

Query: 301 MLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSA 360
           +LHLPHFS  SPESIASFL+GAK+L+PR+VTLVEEEI HGPTIDGDYKVQFLDSLERYSA
Sbjct: 301 VLHLPHFSNSSPESIASFLAGAKTLNPRLVTLVEEEIEHGPTIDGDYKVQFLDSLERYSA 360

Query: 361 IYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGL 420
           IYDSLEA IPMKNRAR LVE+VFLGPRISATLRRIGQ + +  A EENCLWGE+LEKMGL
Sbjct: 361 IYDSLEAGIPMKNRARGLVEKVFLGPRISATLRRIGQPQRAATAAEENCLWGERLEKMGL 420

Query: 421 KTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGSSTSSSSSSS 480
           K   ISFANHCQARLL+ LFNDGYRVEELG+NKLVLGWKSKRLLSVSIW SS+SSSSSSS
Sbjct: 421 KAAAISFANHCQARLLVDLFNDGYRVEELGSNKLVLGWKSKRLLSVSIWASSSSSSSSSS 476

BLAST of IVF0023874 vs. NCBI nr
Match: KAG6578977.1 (Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 687 bits (1773), Expect = 7.20e-245
Identity = 371/503 (73.76%), Postives = 409/503 (81.31%), Query Frame = 0

Query: 1   MALAINNPFNDIALSNYSTSTNNSDDHRHLAGNWNYSSPIVDWETFSGTHNDFLDVFDST 60
           MA+A++N FN +A+S+YSTSTNNSDD                         DF D+FDS 
Sbjct: 1   MAMAVDNAFNGVAVSDYSTSTNNSDD-------------------------DFHDLFDSI 60

Query: 61  ICIENPPFLTE-------------------DRDEEDEFKGLRLYHLLIAAADAVFGDHRS 120
           + I+  P+  +                     +EE+EF+ LRLYHLL+A ADA+FGDH+S
Sbjct: 61  MSIDALPYSPQVVEIDGGTNCNSPSNPAEGREEEEEEFRSLRLYHLLMAVADAMFGDHKS 120

Query: 121 CDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLDSAPVSNKTHHHNHHIHHRDDH 180
            +LA VIL+RLNELVS SHGTNLERLTAYYAQAFQDLLD A VS    +  HH+H RDDH
Sbjct: 121 RELAQVILIRLNELVSTSHGTNLERLTAYYAQAFQDLLDCAAVSGGGPNKPHHLH-RDDH 180

Query: 181 TPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAF 240
           +PTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVA+DRRVHIVDY+IMEGIQWASLMQA 
Sbjct: 181 SPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVADDRRVHIVDYNIMEGIQWASLMQAL 240

Query: 241 VS---SPSAPHLRITAISRGANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDES 300
           VS   SP APHLRITAISRG + RR IGTVQETGRRLVAFAASIGQPFSFHQCKLDSDES
Sbjct: 241 VSRKDSPPAPHLRITAISRGPSSRRWIGTVQETGRRLVAFAASIGQPFSFHQCKLDSDES 300

Query: 301 FRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPT 360
           FRPSGLKLV+GEALVVNC+LHLPHFS  SPESIASFL+GAK+L+PR+VTLVEEEI HGPT
Sbjct: 301 FRPSGLKLVRGEALVVNCVLHLPHFSNTSPESIASFLAGAKTLNPRLVTLVEEEIEHGPT 360

Query: 361 IDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRISATLRRIGQRRWST 420
           IDGDYKVQFLDSLERYSAIYDSLEA IPMKNRAR LVE+VFLGPRISATLRRIGQ + + 
Sbjct: 361 IDGDYKVQFLDSLERYSAIYDSLEAGIPMKNRARGLVEKVFLGPRISATLRRIGQPQRAA 420

Query: 421 AAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLFNDGYRVEELGNNKLVLGWKSKR 480
            A EENCLWGE+LEKMGLK   ISFANHCQARLL+ LFNDGYRVEELG+NKLVLGWKSKR
Sbjct: 421 TAAEENCLWGERLEKMGLKAAAISFANHCQARLLVDLFNDGYRVEELGSNKLVLGWKSKR 476

BLAST of IVF0023874 vs. TAIR 10
Match: AT4G08250.1 (GRAS family transcription factor )

HSP 1 Score: 414.1 bits (1063), Expect = 1.6e-115
Identity = 220/396 (55.56%), Postives = 281/396 (70.96%), Query Frame = 0

Query: 77  DEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQ 136
           DE KGLRL HLL+AAADA  G ++S +L  VIL RL +LVSP   TN+ERL A++     
Sbjct: 97  DESKGLRLVHLLVAAADASTGANKSRELTRVILARLKDLVSPGDRTNMERLAAHFTNGLS 156

Query: 137 DLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQAILEAVA 196
            LL+   V     H +      D +   DV++AF+LLQ MSPYV FG+ TA QAILEAV 
Sbjct: 157 KLLERDSVLCPQQHRD------DVYDQADVISAFELLQNMSPYVNFGYLTATQAILEAVK 216

Query: 197 EDRRVHIVDYDIMEGIQWASLMQAFVS---SPSAPHLRITAISRGANGRRSIGTVQETGR 256
            +RR+HIVDYDI EG+QWASLMQA VS    PSA HLRITA+SR  NG++S+  VQETGR
Sbjct: 217 YERRIHIVDYDINEGVQWASLMQALVSRNTGPSAQHLRITALSRATNGKKSVAAVQETGR 276

Query: 257 RLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIAS 316
           RL AFA SIGQPFS+  CKLD++ +F  S LKLV+GEA+V+NCMLHLP FS+++P S+ S
Sbjct: 277 RLTAFADSIGQPFSYQHCKLDTN-AFSTSSLKLVRGEAVVINCMLHLPRFSHQTPSSVIS 336

Query: 317 FLSGAKSLSPRIVTLVEEEIGHGPTIDGD--YKVQFLDSLERYSAIYDSLEAVIPMKNRA 376
           FLS AK+L+P++VTLV EE+G    + G+  +  +F+D L ++SAI+DSLEA + + N A
Sbjct: 337 FLSEAKTLNPKLVTLVHEEVG----LMGNQGFLYRFMDLLHQFSAIFDSLEAGLSIANPA 396

Query: 377 RALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARL 436
           R  VERVF+GP ++  L RI     + A +E    W + LE  G K +++SF N CQA+L
Sbjct: 397 RGFVERVFIGPWVANWLTRITA---NDAEVESFASWPQWLETNGFKPLEVSFTNRCQAKL 456

Query: 437 LLGLFNDGYRVEELGNNKLVLGWKSKRLLSVSIWGS 468
           LL LFNDG+RVEELG N LVLGWKS+RL+S S W S
Sbjct: 457 LLSLFNDGFRVEELGQNGLVLGWKSRRLVSASFWAS 478

BLAST of IVF0023874 vs. TAIR 10
Match: AT3G54220.1 (GRAS family transcription factor )

HSP 1 Score: 191.0 bits (484), Expect = 2.2e-48
Identity = 137/402 (34.08%), Postives = 216/402 (53.73%), Query Frame = 0

Query: 73  RDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYA 132
           R ++DE +GL L  LL+  A+AV  D  + + A+ +L+ +++L +P +GT+ +R+ AY++
Sbjct: 280 RQKQDE-EGLHLLTLLLQCAEAVSAD--NLEEANKLLLEISQLSTP-YGTSAQRVAAYFS 339

Query: 133 QAFQ-DLLDS-----APVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFT 192
           +A    LL+S     A + ++     H +          +++AFQ+   +SP VKF HFT
Sbjct: 340 EAMSARLLNSCLGIYAALPSRWMPQTHSL---------KMVSAFQVFNGISPLVKFSHFT 399

Query: 193 ANQAILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSA-PHLRITAISRGANGRRSI 252
           ANQAI EA  ++  VHI+D DIM+G+QW  L     S P   PH+R+T +        S+
Sbjct: 400 ANQAIQEAFEKEDSVHIIDLDIMQGLQWPGLFHILASRPGGPPHVRLTGLG------TSM 459

Query: 253 GTVQETGRRLVAFAASIGQPFSFHQCKL-DSDESFRPSGLKLVKGEALVVNCMLHLPHFS 312
             +Q TG+RL  FA  +G PF F  C L +   +     L + K EA+ V+    L H  
Sbjct: 460 EALQATGKRLSDFADKLGLPFEF--CPLAEKVGNLDTERLNVRKREAVAVH---WLQHSL 519

Query: 313 YRSPESIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAV 372
           Y    S A  L   + L+P++VT+VE+++ H     G +  +F++++  YSA++DSL A 
Sbjct: 520 YDVTGSDAHTLWLLQRLAPKVVTVVEQDLSHA----GSFLGRFVEAIHYYSALFDSLGAS 579

Query: 373 IPMKNRARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFA 432
              ++  R +VE+  L   I   L   G  R      E    W EK+++ G K + ++  
Sbjct: 580 YGEESEERHVVEQQLLSKEIRNVLAVGGPSRSGEVKFES---WREKMQQCGFKGISLAGN 639

Query: 433 NHCQARLLLGLF-NDGYRVEELGNNKLVLGWKSKRLLSVSIW 466
              QA LLLG+F +DGY + +  N  L LGWK   LL+ S W
Sbjct: 640 AATQATLLLGMFPSDGYTLVD-DNGTLKLGWKDLSLLTASAW 649

BLAST of IVF0023874 vs. TAIR 10
Match: AT1G55580.1 (GRAS family transcription factor )

HSP 1 Score: 177.6 bits (449), Expect = 2.5e-44
Identity = 145/445 (32.58%), Postives = 211/445 (47.42%), Query Frame = 0

Query: 64  ENPPFLTEDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTN 123
           ENPP L              L  LL  AA+ V    +S   A   L+ +  L S  HG +
Sbjct: 21  ENPPPLCIASSSAATSASHHLRRLLFTAANFV---SQSNFTAAQNLLSILSLNSSPHGDS 80

Query: 124 LERLTAYYAQAF--------QD---------LLDSAPVSNKTHHHN----HHIHHRDDHT 183
            ERL   + +A         QD           +   +SN T   +         R  + 
Sbjct: 81  TERLVHLFTKALSVRINRQQQDQTAETVATWTTNEMTMSNSTVFTSSVCKEQFLFRTKNN 140

Query: 184 PTDVLAAFQL-LQEMSPYVKFGHFTANQAILEAVA--EDRRVHIVDYDIMEGIQWASLMQ 243
            +D  + + L L +++P+++FGH TANQAIL+A    ++  +HI+D DI +G+QW  LMQ
Sbjct: 141 NSDFESCYYLWLNQLTPFIRFGHLTANQAILDATETNDNGALHILDLDISQGLQWPPLMQ 200

Query: 244 AFV---SSPSA--PHLRITAISRGANGRRSIGTVQETGRRLVAFAASIGQPFSFHQCKLD 303
           A     S+PS+  P LRIT   R   G      +  TG RL  FA S+G  F FH   + 
Sbjct: 201 ALAERSSNPSSPPPSLRITGCGRDVTG------LNRTGDRLTRFADSLGLQFQFHTLVIV 260

Query: 304 SDE------SFRPSGLKLVKGEALVVNCMLHLPHFSYRSPESIASFLSGAKSLSPRIVTL 363
            ++        R   L  V+GE + VNC+  L        + I  FLS  KSL+ RIVT+
Sbjct: 261 EEDLAGLLLQIRLLALSAVQGETIAVNCVHFLHKIFNDDGDMIGHFLSAIKSLNSRIVTM 320

Query: 364 VEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKNRARALVERVFLGPRI---- 423
            E E  HG   D  +  +F ++++ Y AI+DSLEA +P  +R R  +E+ + G  I    
Sbjct: 321 AEREANHG---DHSFLNRFSEAVDHYMAIFDSLEATLPPNSRERLTLEQRWFGKEILDVV 380

Query: 424 --SATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQARLLLGLF--NDGY 466
               T R+   RR+         +W E +++ G   V I      QA+LLL L   ++GY
Sbjct: 381 AAEETERKQRHRRFE--------IWEEMMKRFGFVNVPIGSFALSQAKLLLRLHYPSEGY 440

BLAST of IVF0023874 vs. TAIR 10
Match: AT3G03450.1 (RGA-like 2 )

HSP 1 Score: 177.2 bits (448), Expect = 3.2e-44
Identity = 129/398 (32.41%), Postives = 200/398 (50.25%), Query Frame = 0

Query: 81  GLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAYYAQAFQDLLD 140
           G+RL H L+A A+A+     + +LA  ++ R+  L     G  + ++  Y+AQA    + 
Sbjct: 177 GVRLVHALVACAEAI--HQENLNLADALVKRVGTLAGSQAGA-MGKVATYFAQALARRI- 236

Query: 141 SAPVSNKTHHHNHHIHHRDDHTPTDVLAA----FQLLQEM-----SPYVKFGHFTANQAI 200
                           +RD    TDV AA    F+ + EM      PY+KF HFTANQAI
Sbjct: 237 ----------------YRDYTAETDVCAAVNPSFEEVLEMHFYESCPYLKFAHFTANQAI 296

Query: 201 LEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSPSA-PHLRITAISRGANGRRSIGTVQE 260
           LEAV   RRVH++D  + +G+QW +LMQA    P   P  R+T I  G     +  ++Q+
Sbjct: 297 LEAVTTARRVHVIDLGLNQGMQWPALMQALALRPGGPPSFRLTGI--GPPQTENSDSLQQ 356

Query: 261 TGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKL-VKGEALVVNCMLHLPHFSYRSPE 320
            G +L  FA ++G  F F     +S     P   +   + E LVVN +  L     RS  
Sbjct: 357 LGWKLAQFAQNMGVEFEFKGLAAESLSDLEPEMFETRPESETLVVNSVFELHRLLARS-G 416

Query: 321 SIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKN 380
           SI   L+  K++ P IVT+VE+E  H   +  D   +F ++L  YS+++DSLE    + +
Sbjct: 417 SIEKLLNTVKAIKPSIVTVVEQEANHNGIVFLD---RFNEALHYYSSLFDSLEDSYSLPS 476

Query: 381 RARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQA 440
           + R + E V+LG +I   +   G  R      E    W  +++  G   + +  +   QA
Sbjct: 477 QDRVMSE-VYLGRQILNVVAAEGSDR--VERHETAAQWRIRMKSAGFDPIHLGSSAFKQA 536

Query: 441 RLLLGLF--NDGYRVEELGNNKLVLGWKSKRLLSVSIW 466
            +LL L+   DGYRVEE  +  L++GW+++ L++ S W
Sbjct: 537 SMLLSLYATGDGYRVEE-NDGCLMIGWQTRPLITTSAW 544

BLAST of IVF0023874 vs. TAIR 10
Match: AT5G41920.1 (GRAS family transcription factor )

HSP 1 Score: 173.7 bits (439), Expect = 3.6e-43
Identity = 128/399 (32.08%), Postives = 199/399 (49.87%), Query Frame = 0

Query: 71  EDRDEEDEFKGLRLYHLLIAAADAVFGDHRSCDLAHVILVRLNELVSPSHGTNLERLTAY 130
           E+  E D    ++L  LL+  A+ V  DH     A  +L  ++E+ SP  G++ ER+ AY
Sbjct: 27  EETLENDGAAAIKLLSLLLQCAEYVATDH--LREASTLLSEISEICSP-FGSSPERVVAY 86

Query: 131 YAQAFQDLLDSAPVSNKTHHHNHHIHHRDDHTPTDVLAAFQLLQEMSPYVKFGHFTANQA 190
           +AQA Q  + S+ +S      +             + +A Q    +SP +KF HFTANQA
Sbjct: 87  FAQALQTRVISSYLSGACSPLSE--KPLTVVQSQKIFSALQTYNSVSPLIKFSHFTANQA 146

Query: 191 ILEAVAEDRRVHIVDYDIMEGIQWASLMQAFVSSP-SAPHLRITAISRGANGRRSIGTVQ 250
           I +A+  +  VHI+D D+M+G+QW +L     S P     +RIT     ++       + 
Sbjct: 147 IFQALDGEDSVHIIDLDVMQGLQWPALFHILASRPRKLRSIRITGFGSSSD------LLA 206

Query: 251 ETGRRLVAFAASIGQPFSFHQCKLDSDESFRPSGLKLVKGEALVVNCMLHLPHFSYRSPE 310
            TGRRL  FA+S+  PF FH  +        PS L   +GEA+VV+ M    H  Y    
Sbjct: 207 STGRRLADFASSLNLPFEFHPIEGIIGNLIDPSQLATRQGEAVVVHWM---QHRLYDVTG 266

Query: 311 SIASFLSGAKSLSPRIVTLVEEEIGHGPTIDGDYKVQFLDSLERYSAIYDSLEAVIPMKN 370
           +    L   + L P ++T+VE+E+ +     G +  +F+++L  YSA++D+L   +  ++
Sbjct: 267 NNLETLEILRRLKPNLITVVEQELSYDD--GGSFLGRFVEALHYYSALFDALGDGLGEES 326

Query: 371 RARALVERVFLGPRISATLRRIGQRRWSTAAMEENCLWGEKLEKMGLKTVKISFANHCQA 430
             R  VE++ LG  I   +   G RR       +   W E+L ++G + V +      QA
Sbjct: 327 GERFTVEQIVLGTEIRNIVAHGGGRR-------KRMKWKEELSRVGFRPVSLRGNPATQA 386

Query: 431 RLLLGLFN-DGYRVEELGNNKLVLGWKSKRLLSVSIWGS 468
            LLLG+   +GY + E  N  L LGWK   LL+ S W S
Sbjct: 387 GLLLGMLPWNGYTLVE-ENGTLRLGWKDLSLLTASAWKS 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5NE246.3e-14654.39Protein NODULATION SIGNALING PATHWAY 2 OS=Medicago truncatula OX=3880 GN=NSP2 PE... [more]
Q2PEG75.6e-14256.12Protein NODULATION SIGNALING PATHWAY 2 OS=Lotus japonicus OX=34305 GN=NSP2 PE=1 ... [more]
Q9SUF52.2e-11455.56Scarecrow-like protein 26 OS=Arabidopsis thaliana OX=3702 GN=SCL26 PE=2 SV=1[more]
Q84Q929.9e-10754.90Protein NODULATION SIGNALING PATHWAY 2 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
G7L1662.2e-5035.29GRAS family protein RAM1 OS=Medicago truncatula OX=3880 GN=RAM1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7V7674.7e-277100.00Nodulation-signaling pathway 2 protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BE544.7e-277100.00nodulation-signaling pathway 2 protein OS=Cucumis melo OX=3656 GN=LOC103488636 P... [more]
A0A0A0KDX43.4e-26797.09GRAS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040600 PE=3 S... [more]
A0A6J1JUC44.8e-19777.32nodulation-signaling pathway 2 protein OS=Cucurbita maxima OX=3661 GN=LOC1114898... [more]
A0A6J1FK486.5e-19473.16nodulation-signaling pathway 2 protein OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
XP_008445682.10.0100.00PREDICTED: nodulation-signaling pathway 2 protein [Cucumis melo] >KAA0063953.1 n... [more]
XP_004140976.10.096.88protein NODULATION SIGNALING PATHWAY 2 [Cucumis sativus] >KGN45971.1 hypothetica... [more]
XP_038884439.10.091.48protein NODULATION SIGNALING PATHWAY 2 [Benincasa hispida][more]
XP_022993952.13.76e-24777.32nodulation-signaling pathway 2 protein [Cucurbita maxima][more]
KAG6578977.17.20e-24573.76Protein NODULATION SIGNALING PATHWAY 2, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
AT4G08250.11.6e-11555.56GRAS family transcription factor [more]
AT3G54220.12.2e-4834.08GRAS family transcription factor [more]
AT1G55580.12.5e-4432.58GRAS family transcription factor [more]
AT3G03450.13.2e-4432.41RGA-like 2 [more]
AT5G41920.13.6e-4332.08GRAS family transcription factor [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005202Transcription factor GRASPFAMPF03514GRAScoord: 85..465
e-value: 1.6E-95
score: 320.3
IPR005202Transcription factor GRASPANTHERPTHR31636OSJNBA0084A10.13 PROTEIN-RELATEDcoord: 72..467
IPR005202Transcription factor GRASPROSITEPS50985GRAScoord: 75..466
score: 70.790001
IPR030028Scarecrow-like protein 26/nodulation signalling pathway 2PANTHERPTHR31636:SF25SCARECROW-LIKE PROTEIN 26coord: 72..467

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0023874.1IVF0023874.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding