IVF0012436 (gene) Melon (IVF77) v1

Overview
NameIVF0012436
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionEukaryotic aspartyl protease family protein
Locationchr02: 19502193 .. 19503764 (+)
RNA-Seq ExpressionIVF0012436
SyntenyIVF0012436
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGATTCTGTTAGCTTCTCTCCATCATCTTTTACCTTCCCTAACCTTGGCATTTTATTTGTCAACAGCCATAATCTTGTCCACATCGATCATGACAAAGCCTTCACGTTTGGCAACCAAGCTGATCCACCGCAACTCATATCTACATCCACTCTATGACCCAAATGAGACAGTTGAGGATCGATCAAAGAGAGAGCAGGCAAGCTCAATCGAACGCTTTGCTTTTCTCGAGTCAAAGATTAAAGAACTGAAGTCTGTTGGTAATGAAGCTCGATCAAGTCTCATTCCTTTCAATCGAGGTAGTGGGTTTCTTGTTAATTTGTCTATCGGTTCGCCGCCCGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAATGTTTGCCTTGTATCAACTGTTTTCAACAATCGACCTCATGGTTCGATCCTTTGAAATCAGCAAGTTTCAAAACATTGGGTTGTGGATTTCCTGGGTATAACTACATTAATGGTTACAAATGCAATGGTAATCAAGCTGAGTACAAGTTGAGATACCTTGGTGGGGATTCCTCACAAGGAATTCTTGCTAAGGAATCACTTCTCTTTGAGACACTTGATGAAGGTGGAGTTTTTCAATACAATGCTCTATTTCAACACAAACAAGTTAATAAATAAATTTGACATTATTAATTGATTTAGTAATATATTTTGCCGTTTTTATAATTATTATTTTGTGATATCATATGATTTGTGATTATAACAAAGAAAAACTATGACTTTAAATTTGTCGAAGTCACAATGGCTAAAATCAGTATTCGATTGTTGTAGGCAAAATAAAAAAGACGAATTTAACATTTGGGTGTGGTCATATGAACTTCAAAACCAACAATGACGATACCTACAATGGCGTATTTGGATTAGGAGCATATCCCCACATAACAATGGCCACTCAATTAGGCAACAAATTTCCTATTGCATTGGCGATATAAACAATCCTCTCTACACTCACAATCATCTCGTCTTAGGACAAGGATCTTACATTGAAGGCGATTCCACTCCTCTTCAAATCCACTTTGGCCATTATTATGTCACTTTACAAACCATCAGTGTTGGCTCTAAGACCCTCAAAATCGACCCAAATGCCTTCAAGATCTCATCGGACGGCAGCGGTGGAGTTCTGATCGACTCTGGAATGACCTACACTAAGCTTGCGAATGGTGGGTTTGAATTACTTTATGATGAGATTGTTGATTTGATGAAGGGTTTGTTGGAACGAATCCCAACTCAGAGGAAATTTGAAGGGTTGTGTTTTAAAGGAGTTGTGAGTCGAGATCTTGTTGGTTTCCGGCGGTGACGTTTCATTTCGCCGGCGGTGCTGATTTGGTGTTGGAATCGGGGAGTTTGTTCCGGCAACACGGTGGGGATCGGTTTTGTTTAGCTATTTTGCCGAGTAATTCCGAGTTATTGAATCTGTCTGTGATTGGGATTTTGGCTCAACAGAATTATAATGTAGGTTTTGATCTTGAACAAATGAAAGTGTTCTTTCGTAGGATTGATTGTCAACTTCTTGATGAGTAA

mRNA sequence

ATGATGATTCTGTTAGCTTCTCTCCATCATCTTTTACCTTCCCTAACCTTGGCATTTTATTTGTCAACAGCCATAATCTTGTCCACATCGATCATGACAAAGCCTTCACGTTTGGCAACCAAGCTGATCCACCGCAACTCATATCTACATCCACTCTATGACCCAAATGAGACAGTTGAGGATCGATCAAAGAGAGAGCAGGCAAGCTCAATCGAACGCTTTGCTTTTCTCGAGTCAAAGATTAAAGAACTGAAGTCTGTTGGTAATGAAGCTCGATCAAGTCTCATTCCTTTCAATCGAGGTAGTGGGTTTCTTGTTAATTTGTCTATCGGTTCGCCGCCCGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAATGTTTGCCTTGTATCAACTGTTTTCAACAATCGACCTCATGGTTCGATCCTTTGAAATCAGCAAGTTTCAAAACATTGGGTTGTGGATTTCCTGGGTATAACTACATTAATGGTTACAAATGCAATGGTAATCAAGCTGAGTACAAGTTGAGATACCTTGGTGGGGATTCCTCACAAGGAATTCTTGCTAAGGAATCACTTCTCTTTGAGACACTTGATGAAGGCAAAATAAAAAAGACGAATTTAACATTTGGGTGTGGTCATATGAACTTCAAAACCAACAATGACGATACCTACAATGGCGTATTTGGATTAGGAGCATATCCCCACATAACAATGGCCACTCAATTAGGACAAGGATCTTACATTGAAGGCGATTCCACTCCTCTTCAAATCCACTTTGGCCATTATTATGTCACTTTACAAACCATCAGTGTTGGCTCTAAGACCCTCAAAATCGACCCAAATGCCTTCAAGATCTCATCGGACGGCAGCGGTGGAGTTCTGATCGACTCTGGAATGACCTACACTAAGCTTGCGAATGGTGGGTTTGAATTACTTTATGATGAGATTGTTGATTTGATGAAGGGTTTGTTGGAACGAATCCCAACTCAGAGGAAATTTGAAGGATCTTGTTGGTTTCCGGCGGTGACGTTTCATTTCGCCGGCGGTGCTGATTTGGTGTTGGAATCGGGGAGTTTGTTCCGGCAACACGGTGGGGATCGGTTTTGTTTAGCTATTTTGCCGAGTAATTCCGAGTTATTGAATCTGTCTGTGATTGGGATTTTGGCTCAACAGAATTATAATGTAGGTTTTGATCTTGAACAAATGAAAGTGTTCTTTCGTAGGATTGATTGTCAACTTCTTGATGAGTAA

Coding sequence (CDS)

ATGATGATTCTGTTAGCTTCTCTCCATCATCTTTTACCTTCCCTAACCTTGGCATTTTATTTGTCAACAGCCATAATCTTGTCCACATCGATCATGACAAAGCCTTCACGTTTGGCAACCAAGCTGATCCACCGCAACTCATATCTACATCCACTCTATGACCCAAATGAGACAGTTGAGGATCGATCAAAGAGAGAGCAGGCAAGCTCAATCGAACGCTTTGCTTTTCTCGAGTCAAAGATTAAAGAACTGAAGTCTGTTGGTAATGAAGCTCGATCAAGTCTCATTCCTTTCAATCGAGGTAGTGGGTTTCTTGTTAATTTGTCTATCGGTTCGCCGCCCGTGACACAGCTCGTAGTGGTCGACACTGGTAGCTCCCTCCTGTGGGTGCAATGTTTGCCTTGTATCAACTGTTTTCAACAATCGACCTCATGGTTCGATCCTTTGAAATCAGCAAGTTTCAAAACATTGGGTTGTGGATTTCCTGGGTATAACTACATTAATGGTTACAAATGCAATGGTAATCAAGCTGAGTACAAGTTGAGATACCTTGGTGGGGATTCCTCACAAGGAATTCTTGCTAAGGAATCACTTCTCTTTGAGACACTTGATGAAGGCAAAATAAAAAAGACGAATTTAACATTTGGGTGTGGTCATATGAACTTCAAAACCAACAATGACGATACCTACAATGGCGTATTTGGATTAGGAGCATATCCCCACATAACAATGGCCACTCAATTAGGACAAGGATCTTACATTGAAGGCGATTCCACTCCTCTTCAAATCCACTTTGGCCATTATTATGTCACTTTACAAACCATCAGTGTTGGCTCTAAGACCCTCAAAATCGACCCAAATGCCTTCAAGATCTCATCGGACGGCAGCGGTGGAGTTCTGATCGACTCTGGAATGACCTACACTAAGCTTGCGAATGGTGGGTTTGAATTACTTTATGATGAGATTGTTGATTTGATGAAGGGTTTGTTGGAACGAATCCCAACTCAGAGGAAATTTGAAGGATCTTGTTGGTTTCCGGCGGTGACGTTTCATTTCGCCGGCGGTGCTGATTTGGTGTTGGAATCGGGGAGTTTGTTCCGGCAACACGGTGGGGATCGGTTTTGTTTAGCTATTTTGCCGAGTAATTCCGAGTTATTGAATCTGTCTGTGATTGGGATTTTGGCTCAACAGAATTATAATGTAGGTTTTGATCTTGAACAAATGAAAGTGTTCTTTCGTAGGATTGATTGTCAACTTCTTGATGAGTAA

Protein sequence

MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVEDRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYKLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITMATQLGQGSYIEGDSTPLQIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQRKFEGSCWFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Homology
BLAST of IVF0012436 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 2.2e-41
Identity = 132/456 (28.95%), Postives = 217/456 (47.59%), Query Frame = 0

Query: 15  LTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVEDRSKREQASSIERF 74
           + L F+L  ++ LS+S    P   + +LIHR+S L P+Y+P  TV DR       S+ R 
Sbjct: 5   ILLCFFLFFSVTLSSS--GHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRS 64

Query: 75  AFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLP 134
                ++ +      + +S LI       F ++++IG+PP+    + DTGS L WVQC P
Sbjct: 65  RRFNHQLSQ-----TDLQSGLI--GADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKP 124

Query: 135 CINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYK--CNGNQAEYKLRYLGGDS--SQ 194
           C  C++++   FD  KS+++K+  C       ++  +  C+ +    K RY  GD   S+
Sbjct: 125 CQQCYKENGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSK 184

Query: 195 GILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITMATQLGQ 254
           G +A E++  ++     +      FGCG+ N  T  D+T +G+ GLG   H+++ +QLG 
Sbjct: 185 GDVATETVSIDSASGSPVSFPGTVFGCGYNNGGT-FDETGSGIIGLGG-GHLSLISQLGS 244

Query: 255 G------------------------------SYIEGD----STPL--QIHFGHYYVTLQT 314
                                          S +  D    STPL  +    +YY+TL+ 
Sbjct: 245 SISKKFSYCLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEA 304

Query: 315 ISVGSKTL-----KIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGL 374
           ISVG K +       +PN   I S+ SG ++IDSG T T L  G F+     + + + G 
Sbjct: 305 ISVGKKKIPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTG- 364

Query: 375 LERIPTQRKFEGSCW--------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPS 418
            +R+   +     C+         P +T HF  GAD+ L   + F +   D  CL+++P+
Sbjct: 365 AKRVSDPQGLLSHCFKSGSAEIGLPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMVPT 424

BLAST of IVF0012436 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 4.5e-39
Identity = 134/450 (29.78%), Postives = 199/450 (44.22%), Query Frame = 0

Query: 11  LLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVEDRSKREQASS 70
           L  S+ L+  L +++ LS +           LIHR+S   P Y+P ET   R +     S
Sbjct: 4   LFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRS 63

Query: 71  IER-FAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLW 130
           + R F F E          N  +  +   +    +L+N+SIG+PP   + + DTGS LLW
Sbjct: 64  VNRVFHFTEK--------DNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLW 123

Query: 131 VQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYI-NGYKC--NGNQAEYKLRYLGG 190
            QC PC +C+ Q    FDP  S+++K + C       + N   C  N N   Y L Y   
Sbjct: 124 TQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDN 183

Query: 191 DSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITMAT 250
             ++G +A ++L   + D   ++  N+  GCGH N  T N    +G+ GLG  P +++  
Sbjct: 184 SYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKG-SGIVGLGGGP-VSLIK 243

Query: 251 QLGQG-----SYI------EGDSTPLQIHFG-----------------------HYYVTL 310
           QLG       SY       + D T  +I+FG                        YY+TL
Sbjct: 244 QLGDSIDGKFSYCLVPLTSKKDQTS-KINFGTNAIVSGSGVVSTPLIAKASQETFYYLTL 303

Query: 311 QTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLER 370
           ++ISVGSK ++   +    S    G ++IDSG T T L    +  L D +   +    ++
Sbjct: 304 KSISVGSKQIQYSGSD---SESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQ 363

Query: 371 IPTQ-----RKFEGSCWFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 418
            P           G    P +T HF  GAD+ L+S + F Q   D  C A   S S    
Sbjct: 364 DPQSGLSLCYSATGDLKVPVITMHF-DGADVKLDSSNAFVQVSEDLVCFAFRGSPS---- 423

BLAST of IVF0012436 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.5e-37
Identity = 115/356 (32.30%), Postives = 163/356 (45.79%), Query Frame = 0

Query: 104 FLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPG 163
           +L+NLSIG+P      ++DTGS L+W QC PC  CF QST  F+P  S+SF TL C    
Sbjct: 95  YLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQL 154

Query: 164 YNYINGYKCNGNQAEYKLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMN-- 223
              ++   C+ N  +Y   Y  G  +QG +  E+L F     G +   N+TFGCG  N  
Sbjct: 155 CQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTF-----GSVSIPNITFGCGENNQG 214

Query: 224 FKTNNDDTYNGVFGLGAYPHITMATQLG--QGSYIE---GDSTPLQIHFG---------- 283
           F   N     G+ G+G  P +++ +QL   + SY     G STP  +  G          
Sbjct: 215 FGQGNG---AGLVGMGRGP-LSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGS 274

Query: 284 -------------HYYVTLQTISVGSKTLKIDPNAFKI-SSDGSGGVLIDSGMTYTKLAN 343
                         YY+TL  +SVGS  L IDP+AF + S++G+GG++IDSG T T   N
Sbjct: 275 PNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVN 334

Query: 344 GGFELLYDEIVDLMK-----------GLLERIPTQRKFEGSCWFPAVTFHFAGGADLVLE 403
             ++ +  E +  +             L  + P+      +   P    HF GG DL L 
Sbjct: 335 NAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSD---PSNLQIPTFVMHFDGG-DLELP 394

Query: 404 SGSLFRQHGGDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDC 418
           S + F        CLA+    S    +S+ G + QQN  V +D     V F    C
Sbjct: 395 SENYFISPSNGLICLAM---GSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of IVF0012436 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.8e-35
Identity = 130/458 (28.38%), Postives = 205/458 (44.76%), Query Frame = 0

Query: 12  LPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE-DRSKREQASS 71
           L S+ L   + +AI+  TS  ++ +     L+H     H    P   +  D  + +   +
Sbjct: 5   LYSVVLGLAIVSAIVAPTSSTSRGT-----LLH-----HGQKRPQPGLRVDLEQVDSGKN 64

Query: 72  IERFAFLESKIK----ELKSVGNEARSSL---IPFNRGSG-FLVNLSIGSPPVTQLVVVD 131
           + ++  ++  IK     ++S+    +SS     P   G G +L+N++IG+P  +   ++D
Sbjct: 65  LTKYELIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMD 124

Query: 132 TGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYKLR 191
           TGS L+W QC PC  CF Q T  F+P  S+SF TL C       +    CN N+ +Y   
Sbjct: 125 TGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYG 184

Query: 192 YLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMN--FKTNNDDTYNGVFGLGAYP 251
           Y  G ++QG +A E+  FET         N+ FGCG  N  F   N     G+ G+G  P
Sbjct: 185 YGDGSTTQGYMATETFTFET-----SSVPNIAFGCGEDNQGFGQGNG---AGLIGMGWGP 244

Query: 252 HITMATQLGQGSY-----IEGDSTPLQIHFG-----------------------HYYVTL 311
            +++ +QLG G +       G S+P  +  G                       +YY+TL
Sbjct: 245 -LSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITL 304

Query: 312 QTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLER 371
           Q I+VG   L I  + F++  DG+GG++IDSG T T L    +  +     D +      
Sbjct: 305 QGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQI-----N 364

Query: 372 IPT------------QRKFEGS-CWFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAIL 418
           +PT            Q+  +GS    P ++  F GG   + E   L     G   CLA+ 
Sbjct: 365 LPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGGVLNLGEQNILISPAEG-VICLAM- 424

BLAST of IVF0012436 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.4e-32
Identity = 102/348 (29.31%), Postives = 163/348 (46.84%), Query Frame = 0

Query: 99  NRGSG-FLVNLSIGSPPVTQLVVVDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTL 158
           ++GSG +   + +G+P     +V+DTGS + W+QC PC +C+QQS   F+P  S+++K+L
Sbjct: 156 SQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSL 215

Query: 159 GCGFPGYNYINGYKCNGNQAEYKLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGC 218
            C  P  + +    C  N+  Y++ Y  G  + G LA +++ F   + GKI   N+  GC
Sbjct: 216 TCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFG--NSGKI--NNVALGC 275

Query: 219 GHMNFKTNNDDTYNGVFGL----GAYPHITMATQLGQGSY--IEGDS--------TPLQI 278
           GH     +N+  + G  GL    G    IT   +    SY  ++ DS          +Q+
Sbjct: 276 GH-----DNEGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLDFNSVQL 335

Query: 279 HFG--------------HYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTK 338
             G               YYV L   SVG + + +    F + + GSGGV++D G   T+
Sbjct: 336 GGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTR 395

Query: 339 LANGGFELLYDEIVDLMKGLLERIPTQRKFEGSCW---------FPAVTFHFAGGADLVL 398
           L    +  L D  + L   L +   +   F+ +C+          P V FHF GG  L L
Sbjct: 396 LQTQAYNSLRDAFLKLTVNLKKGSSSISLFD-TCYDFSSLSTVKVPTVAFHFTGGKSLDL 455

Query: 399 ESGS-LFRQHGGDRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQ 408
            + + L        FC A  P++S   +LS+IG + QQ   + +DL +
Sbjct: 456 PAKNYLIPVDDSGTFCFAFAPTSS---SLSIIGNVQQQGTRITYDLSK 490

BLAST of IVF0012436 vs. ExPASy TrEMBL
Match: A0A5D3DZ20 (Peptidase A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G002350 PE=3 SV=1)

HSP 1 Score: 822.4 bits (2123), Expect = 8.7e-235
Identity = 420/455 (92.31%), Postives = 421/455 (92.53%), Query Frame = 0

Query: 1   MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 390 MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 449

Query: 61  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 450 DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 509

Query: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 180
           VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK
Sbjct: 510 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 569

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 240
           LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP
Sbjct: 570 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 629

Query: 241 HITMATQ-----------------------LGQGSYIEGDSTPLQIHFGHYYVTLQTISV 300
           HITMATQ                       LGQGSYIEGDSTPLQIHFGHYYVTLQTISV
Sbjct: 630 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTISV 689

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR
Sbjct: 690 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 749

Query: 361 KFEGSCW----------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEG C+          FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN
Sbjct: 750 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 809

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 423
           LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 810 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

BLAST of IVF0012436 vs. ExPASy TrEMBL
Match: A0A5A7UU11 (Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G005510 PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.1e-233
Identity = 418/455 (91.87%), Postives = 420/455 (92.31%), Query Frame = 0

Query: 1   MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILLASLHHLLPSLTLAFYLSTAIILSTSI TKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 390 MMILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVE 449

Query: 61  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 450 DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 509

Query: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 180
           VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK
Sbjct: 510 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 569

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 240
           LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP
Sbjct: 570 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 629

Query: 241 HITMATQ-----------------------LGQGSYIEGDSTPLQIHFGHYYVTLQTISV 300
           +ITMATQ                       LGQGSYIEGDSTPLQIHFGHYYVTLQTISV
Sbjct: 630 YITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTISV 689

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR
Sbjct: 690 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 749

Query: 361 KFEGSCW----------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEG C+          FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN
Sbjct: 750 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 809

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 423
           LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 810 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

BLAST of IVF0012436 vs. ExPASy TrEMBL
Match: A0A1S3BY28 (aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103494349 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.4e-229
Identity = 416/471 (88.32%), Postives = 419/471 (88.96%), Query Frame = 0

Query: 1   MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILLASLHHLLPSLTLAFYLSTAIILSTSI TKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 14  MMILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVE 73

Query: 61  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 74  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 133

Query: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 180
           VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK
Sbjct: 134 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 193

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKI----------------KKTNLTFGCGHMNFKT 240
           LRYLGGDSSQGILAKESLLFETLDEG +                KKTNLTFGCGHMNFKT
Sbjct: 194 LRYLGGDSSQGILAKESLLFETLDEGGVFQYNALFQHKQVNKQNKKTNLTFGCGHMNFKT 253

Query: 241 NNDDTYNGVFGLGAYPHITMATQ-----------------------LGQGSYIEGDSTPL 300
           NNDDTYNGVFGLGAYP+ITMATQ                       LGQGSYIEGDSTPL
Sbjct: 254 NNDDTYNGVFGLGAYPYITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPL 313

Query: 301 QIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE 360
           QIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE
Sbjct: 314 QIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE 373

Query: 361 IVDLMKGLLERIPTQRKFEGSCW----------FPAVTFHFAGGADLVLESGSLFRQHGG 420
           IVDLMKGLLERIPTQRKFEG C+          FPAVTFHFAGGADLVLESGSLFRQHGG
Sbjct: 374 IVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGG 433

Query: 421 DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 423
           DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 434 DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 484

BLAST of IVF0012436 vs. ExPASy TrEMBL
Match: A0A0A0LUP5 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G424350 PE=3 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.1e-225
Identity = 407/456 (89.25%), Postives = 411/456 (90.13%), Query Frame = 0

Query: 1   MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILLASLHHLLPSLTLAFYLSTAII ST I TKPSRLATKLIHRNSYLHPLYD NETVE
Sbjct: 1   MMILLASLHHLLPSLTLAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVE 60

Query: 61  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           DRSKREQ SSIERF FLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 61  DRSKREQTSSIERFDFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120

Query: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEY 180
           VDTGSSLLWVQCLPCINCFQQSTSWFDPLKS SFKTLGCGFPGYNYINGYKCN  NQAEY
Sbjct: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEY 180

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 240
           KLRYLGGDSSQGILAKESLLFETLDEGKIKK+N+TFGCGHMN KTNNDD YNGVFGLGAY
Sbjct: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKSNITFGCGHMNIKTNNDDAYNGVFGLGAY 240

Query: 241 PHITMATQ-----------------------LGQGSYIEGDSTPLQIHFGHYYVTLQTIS 300
           PHITMATQ                       LGQGSYIEGDSTPLQIHFGHYYVTLQ+IS
Sbjct: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSIS 300

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
           VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ
Sbjct: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360

Query: 361 RKFEGSCW----------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420
           RKFEG C+          FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL
Sbjct: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420

Query: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 423
           NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456

BLAST of IVF0012436 vs. ExPASy TrEMBL
Match: A0A6J1C0L1 (probable aspartic protease At2g35615 OS=Momordica charantia OX=3673 GN=LOC111006977 PE=3 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 8.5e-198
Identity = 353/457 (77.24%), Postives = 385/457 (84.25%), Query Frame = 0

Query: 1   MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           M+ LL SLHHLLP LT AFYLSTA+I ST + TKPSRL TKLIHRNSYLHPLYDPNETVE
Sbjct: 1   MVTLLLSLHHLLPFLTSAFYLSTAVISSTDVTTKPSRLVTKLIHRNSYLHPLYDPNETVE 60

Query: 61  DRSKREQASSIERFAFLESKIKELKSV-GNEARSSLIPFNRGSGFLVNLSIGSPPVTQLV 120
           DRSKRE+ SS ERFA+LESKIKEL SV GNEAR++LIPFN+GSGFLVN SIG PPVTQL 
Sbjct: 61  DRSKREETSSTERFAYLESKIKELNSVGGNEARANLIPFNQGSGFLVNFSIGQPPVTQLA 120

Query: 121 VVDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAE 180
           VVDTGSSLLWVQCLPC+NCF+QS SWFDPLKS SFK L C FPG+NY+ GYKCN  +QAE
Sbjct: 121 VVDTGSSLLWVQCLPCVNCFRQSGSWFDPLKSWSFKILDCDFPGHNYVRGYKCNDFHQAE 180

Query: 181 YKLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGA 240
           YKLRYLGGD+S+GILAKESLLFET DEGKI+K NLTFGCGHMN KTN DDTYNGVFGLG 
Sbjct: 181 YKLRYLGGDTSEGILAKESLLFETPDEGKIRKANLTFGCGHMNTKTNRDDTYNGVFGLGG 240

Query: 241 YPHITMATQ-----------------------LGQGSYIEGDSTPLQIHFGHYYVTLQTI 300
           YPHITMATQ                       LG G++IEGDSTPLQI FGHYYV+L+ I
Sbjct: 241 YPHITMATQLGNKFSYCIGDITDPLYAHNHLFLGHGAFIEGDSTPLQIIFGHYYVSLEGI 300

Query: 301 SVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPT 360
           SVGSK LKIDPNAF+++SDG GGVLIDSGMTYTKL NGGFELL+DEI DLMKG+LERIPT
Sbjct: 301 SVGSKRLKIDPNAFQMTSDGRGGVLIDSGMTYTKLTNGGFELLFDEIADLMKGVLERIPT 360

Query: 361 QRKFEGSCW----------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSEL 420
           +RKFEG C+           P VTFHFAGGADLVLESGSLFRQHGGDRFCLA+LPSNSE+
Sbjct: 361 RRKFEGLCYKGVVGRDLVGLPPVTFHFAGGADLVLESGSLFRQHGGDRFCLAVLPSNSEM 420

Query: 421 LNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 423
           +NLSVIG+LAQQNYNVGFDLEQMKVFFRRIDCQLL +
Sbjct: 421 MNLSVIGVLAQQNYNVGFDLEQMKVFFRRIDCQLLGD 457

BLAST of IVF0012436 vs. NCBI nr
Match: TYK28585.1 (Peptidase A1 [Cucumis melo var. makuwa])

HSP 1 Score: 820 bits (2118), Expect = 1.22e-292
Identity = 420/455 (92.31%), Postives = 421/455 (92.53%), Query Frame = 0

Query: 1   MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 390 MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 449

Query: 61  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 450 DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 509

Query: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 180
           VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK
Sbjct: 510 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 569

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 240
           LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP
Sbjct: 570 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 629

Query: 241 HITMATQLG-----------------------QGSYIEGDSTPLQIHFGHYYVTLQTISV 300
           HITMATQLG                       QGSYIEGDSTPLQIHFGHYYVTLQTISV
Sbjct: 630 HITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTISV 689

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR
Sbjct: 690 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 749

Query: 361 KFEGSCW----------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEG C+          FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN
Sbjct: 750 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 809

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 422
           LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 810 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

BLAST of IVF0012436 vs. NCBI nr
Match: XP_008453701.1 (PREDICTED: aspartic proteinase CDR1-like [Cucumis melo])

HSP 1 Score: 802 bits (2071), Expect = 4.05e-291
Identity = 416/471 (88.32%), Postives = 419/471 (88.96%), Query Frame = 0

Query: 1   MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILLASLHHLLPSLTLAFYLSTAIILSTSI TKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 14  MMILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVE 73

Query: 61  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 74  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 133

Query: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 180
           VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK
Sbjct: 134 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 193

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKI----------------KKTNLTFGCGHMNFKT 240
           LRYLGGDSSQGILAKESLLFETLDEG +                KKTNLTFGCGHMNFKT
Sbjct: 194 LRYLGGDSSQGILAKESLLFETLDEGGVFQYNALFQHKQVNKQNKKTNLTFGCGHMNFKT 253

Query: 241 NNDDTYNGVFGLGAYPHITMATQLG-----------------------QGSYIEGDSTPL 300
           NNDDTYNGVFGLGAYP+ITMATQLG                       QGSYIEGDSTPL
Sbjct: 254 NNDDTYNGVFGLGAYPYITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPL 313

Query: 301 QIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE 360
           QIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE
Sbjct: 314 QIHFGHYYVTLQTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDE 373

Query: 361 IVDLMKGLLERIPTQRKFEGSCW----------FPAVTFHFAGGADLVLESGSLFRQHGG 420
           IVDLMKGLLERIPTQRKFEG C+          FPAVTFHFAGGADLVLESGSLFRQHGG
Sbjct: 374 IVDLMKGLLERIPTQRKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGG 433

Query: 421 DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 422
           DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 434 DRFCLAILPSNSELLNLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 484

BLAST of IVF0012436 vs. NCBI nr
Match: KAA0058227.1 (aspartic proteinase CDR1-like [Cucumis melo var. makuwa])

HSP 1 Score: 815 bits (2106), Expect = 8.11e-291
Identity = 418/455 (91.87%), Postives = 420/455 (92.31%), Query Frame = 0

Query: 1   MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILLASLHHLLPSLTLAFYLSTAIILSTSI TKPSRLATKLIHRNSYLHPLYDPNETVE
Sbjct: 390 MMILLASLHHLLPSLTLAFYLSTAIILSTSITTKPSRLATKLIHRNSYLHPLYDPNETVE 449

Query: 61  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 450 DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 509

Query: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 180
           VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK
Sbjct: 510 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNGNQAEYK 569

Query: 181 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 240
           LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP
Sbjct: 570 LRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYP 629

Query: 241 HITMATQLG-----------------------QGSYIEGDSTPLQIHFGHYYVTLQTISV 300
           +ITMATQLG                       QGSYIEGDSTPLQIHFGHYYVTLQTISV
Sbjct: 630 YITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQTISV 689

Query: 301 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 360
           GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR
Sbjct: 690 GSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQR 749

Query: 361 KFEGSCW----------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 420
           KFEG C+          FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN
Sbjct: 750 KFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 809

Query: 421 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 422
           LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 810 LSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 844

BLAST of IVF0012436 vs. NCBI nr
Match: XP_031742409.1 (aspartic proteinase CDR1 [Cucumis sativus] >KGN65478.1 hypothetical protein Csa_020065 [Cucumis sativus])

HSP 1 Score: 789 bits (2037), Expect = 2.15e-286
Identity = 407/456 (89.25%), Postives = 411/456 (90.13%), Query Frame = 0

Query: 1   MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           MMILLASLHHLLPSLTLAFYLSTAII ST I TKPSRLATKLIHRNSYLHPLYD NETVE
Sbjct: 1   MMILLASLHHLLPSLTLAFYLSTAIISSTLITTKPSRLATKLIHRNSYLHPLYDQNETVE 60

Query: 61  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           DRSKREQ SSIERF FLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV
Sbjct: 61  DRSKREQTSSIERFDFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120

Query: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEY 180
           VDTGSSLLWVQCLPCINCFQQSTSWFDPLKS SFKTLGCGFPGYNYINGYKCN  NQAEY
Sbjct: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSVSFKTLGCGFPGYNYINGYKCNRFNQAEY 180

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 240
           KLRYLGGDSSQGILAKESLLFETLDEGKIKK+N+TFGCGHMN KTNNDD YNGVFGLGAY
Sbjct: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKSNITFGCGHMNIKTNNDDAYNGVFGLGAY 240

Query: 241 PHITMATQLG-----------------------QGSYIEGDSTPLQIHFGHYYVTLQTIS 300
           PHITMATQLG                       QGSYIEGDSTPLQIHFGHYYVTLQ+IS
Sbjct: 241 PHITMATQLGNKFSYCIGDINNPLYTHNHLVLGQGSYIEGDSTPLQIHFGHYYVTLQSIS 300

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
           VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ
Sbjct: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360

Query: 361 RKFEGSCW----------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420
           RKFEG C+          FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL
Sbjct: 361 RKFEGLCFKGVVSRDLVGFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420

Query: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 422
           NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE
Sbjct: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 456

BLAST of IVF0012436 vs. NCBI nr
Match: XP_038878960.1 (aspartic proteinase CDR1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 740 bits (1910), Expect = 4.75e-267
Identity = 377/456 (82.68%), Postives = 399/456 (87.50%), Query Frame = 0

Query: 1   MMILLASLHHLLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVE 60
           M+ILL S +HLLPSLTLAFYLSTAII S +  TKPSRLATKLIHRNSYLHPLYDP ET+E
Sbjct: 1   MVILLVSQYHLLPSLTLAFYLSTAIISSMAFTTKPSRLATKLIHRNSYLHPLYDPTETIE 60

Query: 61  DRSKREQASSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 120
           DRSKRE+ SSIERFA+LESKIKELKSVGNEARSSLIPFN+GSGFLVNLSIGSPPVTQLVV
Sbjct: 61  DRSKREETSSIERFAYLESKIKELKSVGNEARSSLIPFNQGSGFLVNLSIGSPPVTQLVV 120

Query: 121 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYKCNG-NQAEY 180
            DTGSSLLWVQCLPCI+CF+QS SWFDPLKS SFK LGCGF GYNYI+GY+CNG NQAEY
Sbjct: 121 ADTGSSLLWVQCLPCIDCFRQSNSWFDPLKSTSFKILGCGFAGYNYISGYRCNGFNQAEY 180

Query: 181 KLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAY 240
           KLRYLGGD+SQG+LAKESLLFETLDEGKIKKTNLTFGCGHMN KTNNDDTYNGVFGLGAY
Sbjct: 181 KLRYLGGDTSQGVLAKESLLFETLDEGKIKKTNLTFGCGHMNSKTNNDDTYNGVFGLGAY 240

Query: 241 PHITMATQLG-----------------------QGSYIEGDSTPLQIHFGHYYVTLQTIS 300
           P+ITMATQLG                       +GSYIEGDSTPLQIHFGHYYVTLQ IS
Sbjct: 241 PYITMATQLGNKFSYCIGDINNPVYTHNHLVLGEGSYIEGDSTPLQIHFGHYYVTLQGIS 300

Query: 301 VGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ 360
           VGSK LKIDP AF+++ DG GGVLIDSGMTYTKLANGGFELLYDEI+DLM GLLERIPT+
Sbjct: 301 VGSKRLKIDPKAFQMTWDGRGGVLIDSGMTYTKLANGGFELLYDEILDLMTGLLERIPTE 360

Query: 361 RKFEGSCW----------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELL 420
           RKFEG C+          FP VTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSE+L
Sbjct: 361 RKFEGLCFKGVVSRDLIGFPTVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSEML 420

Query: 421 NLSVIGILAQQNYNVGFDLEQMKVFFRRIDCQLLDE 422
           NLSVIGILAQQNYNV FDLEQMKVFFRRIDCQLLDE
Sbjct: 421 NLSVIGILAQQNYNVAFDLEQMKVFFRRIDCQLLDE 456

BLAST of IVF0012436 vs. TAIR 10
Match: AT4G30030.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 250.8 bits (639), Expect = 2.0e-66
Identity = 154/399 (38.60%), Postives = 220/399 (55.14%), Query Frame = 0

Query: 62  RSKREQASSIERFAFLESKIKELKSVGN-EARSSLIPFNRGSGFLVNLSIGSPPVTQLVV 121
           R+K +++S I +  +L SK      + N    S + P    + FL N+SIG+PPV QL++
Sbjct: 36  RTKTQESSKI-KIGYLHSKSTPASRLDNLWTVSHVTPIPNPAAFLANISIGNPPVPQLLL 95

Query: 122 VDTGSSLLWVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYK--CNGNQAE 181
           +DTGS L W+ CLPC  C+ Q+  +F P +S++++   C    +     ++    GN  +
Sbjct: 96  IDTGSDLTWIHCLPC-KCYPQTIPFFHPSRSSTYRNASCVSAPHAMPQIFRDEKTGN-CQ 155

Query: 182 YKLRYLGGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLG- 241
           Y LRY    +++GILA+E L FET D+G I K N+ FGCG  N   +    Y+GV GLG 
Sbjct: 156 YHLRYRDFSNTRGILAEEKLTFETSDDGLISKQNIVFGCGQDN---SGFTKYSGVLGLGP 215

Query: 242 ------------------------AYPHITMATQLGQGSYIEGDSTPLQIHFGHYYVTLQ 301
                                    YPH  +   LG G+ IEGD TPLQI    YY+ LQ
Sbjct: 216 GTFSIVTRNFGSKFSYCFGSLTNPTYPHNILI--LGNGAKIEGDPTPLQIFQDRYYLDLQ 275

Query: 302 TISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERI 361
            IS G K L I+P  F+      GG +ID+G + T LA   +E L +EI  L+  +L R+
Sbjct: 276 AISFGEKLLDIEPGTFQ-RYRSQGGTVIDTGCSPTILAREAYETLSEEIDFLLGEVLRRV 335

Query: 362 PTQRKFEGSCW----------FPAVTFHFAGGADLVLESGSLF-RQHGGDRFCLAILPSN 421
               ++   C+          FP VTFHFAGGA+L L+  SLF     GD FCLA+  + 
Sbjct: 336 KDWDQYTTPCYEGNLKLDLYGFPVVTFHFAGGAELALDVESLFVSSESGDSFCLAMTMNT 395

BLAST of IVF0012436 vs. TAIR 10
Match: AT2G23945.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 225.7 bits (574), Expect = 6.9e-59
Identity = 161/457 (35.23%), Postives = 240/457 (52.52%), Query Frame = 0

Query: 11  LLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSY--LHPLYDPNETVEDRSKREQA 70
           LL  +T+++++ T  I       KP+R+A KLIHR S   L+P      T ED  K    
Sbjct: 9   LLLFITVSYFVVTESI-------KPNRMAMKLIHRESVARLNPNARVPITPEDHIKHLTD 68

Query: 71  SSIERFAFLESKI-KELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSL 130
            S  RF +L++ I KEL S  +  +  +    + S FLVN S+G PPV QL ++DTGSSL
Sbjct: 69  ISSARFKYLQNSIDKELGS--SNFQVDVEQAIKTSLFLVNFSVGQPPVPQLTIMDTGSSL 128

Query: 131 LWVQCLPCINCFQQST--SWFDPLKSASFKTLGCGFPGYNYI-NGYKCNGNQAEYKLRYL 190
           LW+QC PC +C         F+P  S++F    C      Y  NG+  + N+  Y+  Y+
Sbjct: 129 LWIQCQPCKHCSSDHMIHPVFNPALSSTFVECSCDDRFCRYAPNGHCGSSNKCVYEQVYI 188

Query: 191 GGDSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITM 250
            G  S+G+LAKE L F T +   +    + FGCG+ N +   +  + G+ GLGA P  ++
Sbjct: 189 SGTGSKGVLAKERLTFTTPNGNTVVTQPIAFGCGYENGE-QLESHFTGILGLGAKP-TSL 248

Query: 251 ATQ-----------------------LGQGSYIEGDSTPLQIHFGH--YYVTLQTISVGS 310
           A Q                       LG+ + I GD TP++    +  YY+ L+ ISVG 
Sbjct: 249 AVQLGSKFSYCIGDLANKNYGYNQLVLGEDADILGDPTPIEFETENSIYYMNLEGISVGD 308

Query: 311 KTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLERIPTQ--- 370
             L I+P  FK     + GV++DSG  YT LA+  +  LY+EI  ++   LER   +   
Sbjct: 309 TQLNIEPVVFKRRGPRT-GVILDSGTLYTWLADIAYRELYNEIKSILDPKLERFWFRDFL 368

Query: 371 ----RKFEGSCWFPAVTFHFAGGADLVLESGSLF----RQHGGDRFCLAILPS---NSEL 423
               R  E    FP VTFHFAGGA+L +E+ S+F      +  + FC+++ P+     E 
Sbjct: 369 CYHGRVSEELIGFPVVTFHFAGGAELAMEATSMFYPLSEPNTFNVFCMSVKPTKEHGGEY 428

BLAST of IVF0012436 vs. TAIR 10
Match: AT4G30040.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 222.2 bits (565), Expect = 7.6e-58
Identity = 143/387 (36.95%), Postives = 201/387 (51.94%), Query Frame = 0

Query: 69  SSIERFAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLL 128
           +S+ER  +L++K              +IP      FLVN+SIGSPP+TQL+ +DT S LL
Sbjct: 54  ASVERLEYLKAKTTGDIIAHLSPNVPIIP----QAFLVNISIGSPPITQLLHMDTASDLL 113

Query: 129 WVQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYIN-GYKCNGNQAEYKLRYLGGD 188
           W+QCLPCINC+ QS   FDP +S + +   C    Y+  +  +  N    EY +RY+   
Sbjct: 114 WIQCLPCINCYAQSLPIFDPSRSYTHRNETCRTSQYSMPSLKFNANTRSCEYSMRYVDDT 173

Query: 189 SSQGILAKESLLFETL--DEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLG-------- 248
            S+GILA+E LLF T+  +       ++ FGCGH N+      T  G+ GLG        
Sbjct: 174 GSKGILAREMLLFNTIYDESSSAALHDVVFGCGHDNYGEPLVGT--GILGLGYGEFSLVH 233

Query: 249 ----------------AYPHITMATQLG-QGSYIEGDSTPLQIHFGHYYVTLQTISVGSK 308
                           +YPH  +   LG  G+ I GD+TPL+IH G YYVT++ ISV   
Sbjct: 234 RFGKKFSYCFGSLDDPSYPHNVLV--LGDDGANILGDTTPLEIHNGFYYVTIEAISVDGI 293

Query: 309 TLKIDPNAF-KISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLER--IPTQR 368
            L IDP  F +    G GG +ID+G + T L    ++ L + I D+ +G      +    
Sbjct: 294 ILPIDPRVFNRNHQTGLGGTIIDTGNSLTSLVEEAYKPLKNRIEDIFEGRFTAADVSQDD 353

Query: 369 KFEGSCW------------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSEL 413
             +  C+            FP VTFHF+ GA+L L+  SLF +   + FCLA+ P     
Sbjct: 354 MIKMECYNGNFERDLVESGFPIVTFHFSEGAELSLDVKSLFMKLSPNVFCLAVTPG---- 413

BLAST of IVF0012436 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 171.4 bits (433), Expect = 1.5e-42
Identity = 132/456 (28.95%), Postives = 217/456 (47.59%), Query Frame = 0

Query: 15  LTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVEDRSKREQASSIERF 74
           + L F+L  ++ LS+S    P   + +LIHR+S L P+Y+P  TV DR       S+ R 
Sbjct: 5   ILLCFFLFFSVTLSSS--GHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRS 64

Query: 75  AFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLWVQCLP 134
                ++ +      + +S LI       F ++++IG+PP+    + DTGS L WVQC P
Sbjct: 65  RRFNHQLSQ-----TDLQSGLI--GADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKP 124

Query: 135 CINCFQQSTSWFDPLKSASFKTLGCGFPGYNYINGYK--CNGNQAEYKLRYLGGDS--SQ 194
           C  C++++   FD  KS+++K+  C       ++  +  C+ +    K RY  GD   S+
Sbjct: 125 CQQCYKENGPIFDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSK 184

Query: 195 GILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITMATQLGQ 254
           G +A E++  ++     +      FGCG+ N  T  D+T +G+ GLG   H+++ +QLG 
Sbjct: 185 GDVATETVSIDSASGSPVSFPGTVFGCGYNNGGT-FDETGSGIIGLGG-GHLSLISQLGS 244

Query: 255 G------------------------------SYIEGD----STPL--QIHFGHYYVTLQT 314
                                          S +  D    STPL  +    +YY+TL+ 
Sbjct: 245 SISKKFSYCLSHKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEA 304

Query: 315 ISVGSKTL-----KIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGL 374
           ISVG K +       +PN   I S+ SG ++IDSG T T L  G F+     + + + G 
Sbjct: 305 ISVGKKKIPYTGSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTG- 364

Query: 375 LERIPTQRKFEGSCW--------FPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPS 418
            +R+   +     C+         P +T HF  GAD+ L   + F +   D  CL+++P+
Sbjct: 365 AKRVSDPQGLLSHCFKSGSAEIGLPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMVPT 424

BLAST of IVF0012436 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 163.7 bits (413), Expect = 3.2e-40
Identity = 134/450 (29.78%), Postives = 199/450 (44.22%), Query Frame = 0

Query: 11  LLPSLTLAFYLSTAIILSTSIMTKPSRLATKLIHRNSYLHPLYDPNETVEDRSKREQASS 70
           L  S+ L+  L +++ LS +           LIHR+S   P Y+P ET   R +     S
Sbjct: 4   LFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRS 63

Query: 71  IER-FAFLESKIKELKSVGNEARSSLIPFNRGSGFLVNLSIGSPPVTQLVVVDTGSSLLW 130
           + R F F E          N  +  +   +    +L+N+SIG+PP   + + DTGS LLW
Sbjct: 64  VNRVFHFTEK--------DNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLW 123

Query: 131 VQCLPCINCFQQSTSWFDPLKSASFKTLGCGFPGYNYI-NGYKC--NGNQAEYKLRYLGG 190
            QC PC +C+ Q    FDP  S+++K + C       + N   C  N N   Y L Y   
Sbjct: 124 TQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDN 183

Query: 191 DSSQGILAKESLLFETLDEGKIKKTNLTFGCGHMNFKTNNDDTYNGVFGLGAYPHITMAT 250
             ++G +A ++L   + D   ++  N+  GCGH N  T N    +G+ GLG  P +++  
Sbjct: 184 SYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKG-SGIVGLGGGP-VSLIK 243

Query: 251 QLGQG-----SYI------EGDSTPLQIHFG-----------------------HYYVTL 310
           QLG       SY       + D T  +I+FG                        YY+TL
Sbjct: 244 QLGDSIDGKFSYCLVPLTSKKDQTS-KINFGTNAIVSGSGVVSTPLIAKASQETFYYLTL 303

Query: 311 QTISVGSKTLKIDPNAFKISSDGSGGVLIDSGMTYTKLANGGFELLYDEIVDLMKGLLER 370
           ++ISVGSK ++   +    S    G ++IDSG T T L    +  L D +   +    ++
Sbjct: 304 KSISVGSKQIQYSGSD---SESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQ 363

Query: 371 IPTQ-----RKFEGSCWFPAVTFHFAGGADLVLESGSLFRQHGGDRFCLAILPSNSELLN 418
            P           G    P +T HF  GAD+ L+S + F Q   D  C A   S S    
Sbjct: 364 DPQSGLSLCYSATGDLKVPVITMHF-DGADVKLDSSNAFVQVSEDLVCFAFRGSPS---- 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3EBM52.2e-4128.95Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561... [more]
Q6XBF84.5e-3929.78Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q766C32.5e-3732.30Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q766C21.8e-3528.38Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LS402.4e-3229.31Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Match NameE-valueIdentityDescription
A0A5D3DZ208.7e-23592.31Peptidase A1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G002350 ... [more]
A0A5A7UU112.1e-23391.87Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S3BY282.4e-22988.32aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103494349 PE=3 SV=1[more]
A0A0A0LUP52.1e-22589.25Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G42435... [more]
A0A6J1C0L18.5e-19877.24probable aspartic protease At2g35615 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
Match NameE-valueIdentityDescription
TYK28585.11.22e-29292.31Peptidase A1 [Cucumis melo var. makuwa][more]
XP_008453701.14.05e-29188.32PREDICTED: aspartic proteinase CDR1-like [Cucumis melo][more]
KAA0058227.18.11e-29191.87aspartic proteinase CDR1-like [Cucumis melo var. makuwa][more]
XP_031742409.12.15e-28689.25aspartic proteinase CDR1 [Cucumis sativus] >KGN65478.1 hypothetical protein Csa_... [more]
XP_038878960.14.75e-26782.68aspartic proteinase CDR1-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G30030.12.0e-6638.60Eukaryotic aspartyl protease family protein [more]
AT2G23945.16.9e-5935.23Eukaryotic aspartyl protease family protein [more]
AT4G30040.17.6e-5836.95Eukaryotic aspartyl protease family protein [more]
AT2G35615.11.5e-4228.95Eukaryotic aspartyl protease family protein [more]
AT5G33340.13.2e-4029.78Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 86..255
e-value: 3.4E-33
score: 117.2
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 256..420
e-value: 1.5E-35
score: 124.4
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 102..418
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 267..413
e-value: 7.1E-24
score: 84.4
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 104..250
e-value: 4.7E-30
score: 105.1
NoneNo IPR availablePANTHERPTHR47967:SF14EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 27..251
coord: 248..419
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 27..251
coord: 248..419
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 119..130
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 104..413
score: 23.257528
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 103..417
e-value: 2.79889E-60
score: 195.174

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0012436.2IVF0012436.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0016757 glycosyltransferase activity