IVF0008879 (gene) Melon (IVF77) v1

Overview
NameIVF0008879
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionAdoMet-dependent rRNA methyltransferase SPB1
Locationchr04: 31641496 .. 31646889 (-)
RNA-Seq ExpressionIVF0008879
SyntenyIVF0008879
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACATGATGACTCTTCCTTCGCTTCTTCAATCACCTATTTCGTCTTGGGAGGAGAGATGTGAGAGTCTAGAGCCCCAGTCCTTTGGACGGGTTTTCGTGTAAGTCTTTTTTTCAATGCTTGGTTAATCCTTCACCTTCAGTTGAGTTGGTCTTTTCATCGATTTGGAGGATTAAGATCCCTAAAAAGGTTCTTACATGGCTTGTCCTTCATTGCCGTATCATCACGTTGGATAGGTTTTCGAGGAAGATGTTGTTGGTTGGGCCTTTTTGCTGTATTCTGTGGAAGGTGGAGGAAGACTTTAGACAATATCTTTTGCGATTGTGAGATGGTGAGCCATGTTATATTTGGGATGCCTTTTTCTAGACTTTTTTCTTGAGCAGTGTTCGTCACAGAATTGTTAAAGATATGATAGAGGATTTCCTCCTCAATCCTCCGTTTGGGAAGAAAGACCGATTTCTCTGGTGTGCTAGTGTCTGCTATTTTGTGGGTGTTATGGGACGAGAGGAATTGTAGGGTGTTTAGTGGAGGCTTATAGTCTCTTGTTTGTTTTCATGTTTCTCATTGAGCTTTGATTTCAAAGATTTTTTGTAATTATCCTATGAACATGATTTTTTATAGTTGGAGTCACTTCTTGTAGAGGGATTCCTCTTTTTTGAGGGCTTGGTTTTATCTGTGCATTCTGTCTTAATGAAAGTTGTGGTTTTTATAAATTAAAAGGGCTAATTTTCAAAACAAAAAATTGAAAAGCTCAATGGTTATCAATCGGAGCTTGAACTTTTCAATTAAATATTTGCTCAATTTTGATTTTATCCTTGAATGTGGCAAACCTCTTGTTATTATTGGAAATTAGGAATTTGATGATTGTGCTCAATTGGATTATCAATGAGGCTTGTTGGATTTATAAAAAAATTGTGAATTTTTTTTATTGAGGGTTCGATATTTTTATTTAATATTAAATACACTTAGACTTCCTTTGATGCCCAACTTTAACTTTCTTGCTGGGTTTGAATGTTGCCAGGAGGCCTGTCTGCTCTGCACTACCGGTAACTCTTGCTTGGTTCTTCAATCATGGGCAAAGCTTCAAGAGATAAGAGGGTTTAAGCTCTATTCTATTTGATTATTTGTATTCCTGATTCATTATCATTTGTTTACTTGGTCTCATTCCTTGGTCTCATTTGCAGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTAGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGTGTGTTCTTGTATTGAGCTCCTCTATTAAAATATTGTTATGTTTCTGGTAGTATGTCAATCACTGAGTTGGTCATGATAATTAATCTTTCTGCAACATTTTGGATTAACCTTTCTAACAGAGTTGTTTTGTGTGATCTTTATAACTTTATAATCATCTTGTTTTATCATGTATAAGTGTTTTGAAATGCTTCTAAGTACATCGTCTACAATGAAATTTTATATGCAGGAGTCAAGAGAGTGGTAGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTAATTTCTTTTCAGCGTCTTCTCGTTGCAATTTGTTTATCAAACTAGTGGTCAATGACTCAATGCCTTATGGGGAATTTTTTTTTTTTGTATAAAACAATAAACCTAAGAAGAATTCCTCTTGCGATAGTCTTTGCAAGCATCTTTTTTGTTTGGAAAGCATGCCTATATAGTGTATATGGGGGTGCAATCCTCTCCTCCCTTCATTGACCCTGGATCTTGGCACACTGAGTATGTTTTGATAAACGCTAAACATAATGAGGGCTGATAAAAGCAATGTAGTATGTGGTGATGCAACTATTGCTTACAATATAGTTTATTGCTGTTAATTATTTCAAAGTTGTTCTGACATTTTCTTGCCTACTAATCATATATAGGTCCTCAGTAGAAAATTGTATCTCCCAGCAAAGCTTTCTCCAGATTTAAAGTAAGCATGGTAGATTATTATATGAAAATGTAGGCCTCATTTATTAATCATCTTGTTTTTGGTTTTTGGTTTTTGAACAGCAAACTTGTTTTCTCTCAATTCCTGACAATGATTTACATTTTTCTTAAGTGAAAGAGTTGAGTTCTTAACCAAATTCCAAAACAAAAACAAGTTTTTCAAGACTATTTTTTTAGTTTCAGAATTTGGCTTGGTTTTTAAAAACAATAGTAAAAACTGAAAAGTAGTCCACGAAGTAGGAAATTTAGAAGTGGAAGGGGTAATAGGCTTAATTTTTAAAAACTGAACACTAAAACCAGATGGTTACCAAATGGGGCATAACTTTTTCCTTCCCATCCTTCTCTCCATTGTCCCCTTGTTATCTCCCTCTTTTATTGATTCTCTAGCTTCATTTGCAGGGATGGCGATCTTCCTCTTATTGTTGCTATTGATTTACAGCCGATGGCTCCAATTGAAGGTGTTATTGAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTAAGCAGTGTGTATCATGTACACACTATTTTTAACATTTGATATGGTAAACGAAGACATATCCAGATCCTAATGCACCAACATGGTCAATTTTGTTTTAAAATTTCCATTTGTGATAATTTTCCTACGTTTTTCTTCATACTGGATTACTTTTTCTTTTGAATGAGTACTCACGCTGTGTTGTAAGTCCTTTTTCTTCTATTAAAAAAATTGTCACATGAAATTGTGATATCCGTTTGTTCATGGTTTATCAAATATTTAATTTTACCAGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGACGGTGCACCTGATGGTAGATATTTGTGTTTTTTGAATTCTCTCTTGGTATTCTACCTGATGATATTTTGTTACTGATTATGTGCTTGTGTTCGTAGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTTCCAGCTCATACTAGCTGTAAGTGCAGTGTTTTTTTGTACAGACTGTTTAATTTCTCTAGATGGTGCTGATGAATAGTTTAAAATATTTTTGAAAATGCATGAGATGCATGCACATTGACAGTAATGATGTTGCTCCAGTTCTCGTTTACAGTGCTGGAGAGGATAAAAAAAATAAAACCAACAAGAAGCACTAATGAGTGTCAAATTGTTTGAATCTACAAATAGAAAATACCGTGTGACAAGTGAGCAAGGTAGATGAGATCCTTTAGGTTAATCGTGGTTGCTGCATATTATGAAGTTATATTGAAATCTTCTAAAGGTTTTTCTATTGCATGTTGCCTGCACTGGTTGACCTTTTTCCTTTATGGATTGAATTTTGTTGCTGTCATCAGTGTCCTCTGCTGCTGCCTCGTTTTCTCTATTTCTCTCTCTTCCTTCAGCTAGTTCTCAATGCAGTAGGAGATATATGAGTTTTACAGAGATGAGCTGGACTTTTTTTCCTTTTCTTCTCAGGGTTTAACAATTGTTACGCACATACTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATAGTCAGTGAATGGTTGTTACAGGACCCAATAGAAATATGTAGCATACTCGTGTTGTAAAGAAATTTATGGTGTTCTCACTAAATGCATTTGCAATATGAATCATAATCTCTCTTTCAACTTTTCTTCTTCCAATCAAAGGGAAATTTTGCTTTGAGCTCCTTTTCCCTGTAATTATTGCATTGACTATATGATTTTTTCAAGTATTATCATGGATTAGATAAGCAGCTTAATCAAGGTGTTTGTTGTTCCATACATGGTTATGAAGCCGCCAATGATTTTTACATTTTTGAGTTTTTCTGTTGCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAGCCAAAAAGCAGTCGGAACTCGAGTATAGGTAACCTTTCGGTTTTCCACTTTTGTTGATTGGTTGCTATAGAAGTCCATCTTTCGATTATAATTGATTTTGTTTCCAGAAAATGATATTCTCTTAATTGAAAATGGCCTCTCTCTCTCTTATTCTTATTTTTTAATCCCTTTATCGCTTCTGGTTCATAGGATTCTTGCGCATGAATTTTTAGATACCCATTGTACTTAGATTCCTTAAATCAACTTTAGATTGTTATCGATTAATACTAGCTGGTCGGACTGGTGTCTGCCCAACAGCAAATTGTGGAGTTGGTACATTTAGGGGTGGTTTGTAATATAAAAAAAGGTGTGCAAGAGGACAAAATAGTCAAGAGTAAAAGTAAAATTTTTGAACTTTCACTGTTGCTTTTGTGGACATCCATGAATCCAAATAGTCCATTATGGTAAGTCTCCTTTTATCACTTGCTTTAATGTTTTAAACAGGTAAGCTGTGATTATTTGGCATTGTGTACCAGAAATAGAGTTTTTTGGTTTTTCTGCCCTTACTTAGTTGGCTTTCTCTTTTATATCACACGGTATATCATACAAGATCATTATTTGTTAACGTTAGTCAAATCAAAGTTCCAAGAAAACATTTAAGCTACGGTATTAACCATAAGAAATTACTAGGATAAAAAATCAAGAGTTCAAGAACATAATTAGAACGTCTGAAAGTTGAATGGACTAAGTGGGCACAAGTATTAAAATTATTGAACTAAATTTGTAATTCATTCCAGTGCTTTCTTTTAGTTGCCTAGGTGAACGCACAGTAACAACTTCTAAACAAAGTTGAGTTAGATTATGATTTAACTTTATGTCGAAGCTCATACGTTGACTGGGTGCAAATGCAGAGGCATTTGCAGTTTGTGAGAACTATTCTCCTCCCGAAGGTTTCAATCCAAAAGATCTGCATCGCTTGCTGGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGGTAAACTCAATATCCTACCTTGATTTGGAGTTCACATTTCGATAGACGTATCCTAAGATCAAGCATGTTCTTATCCTATTTTTAAAGATTAATTTGTTTTACAGCTGGCGCTATGACCGAGCTGTCTACTTGTCTCTAGGCTTTTAATAATTAGTGTGTCTGCTGATTTTGATGTGGTCAATGCTCTGGTATTTTTGGTTACATAAGAGGAAGTAATAGCATTTCTATTTTGCTACATTTTGTCCCTCTCAGATTGCAGTAGTGGATGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATATCCACTGCCCAGAGGTGCTGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATTGCCCCTCCATACAAGAGGGCTCTCGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGA

mRNA sequence

ATGGACATGATGACTCTTCCTTCGCTTCTTCAATCACCTATTTCGTCTTGGGAGGAGAGATGTGAGAGTCTAGAGCCCCAGTCCTTTGGACGGGTTTTCGTGAGGCCTGTCTGCTCTGCACTACCGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTAGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGAGTCAAGAGAGTGGTAGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTCAGTAGAAAATTGTATCTCCCAGCAAAGCTTTCTCCAGATTTAAAGGATGGCGATCTTCCTCTTATTGTTGCTATTGATTTACAGCCGATGGCTCCAATTGAAGGTGTTATTGAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGACGGTGCACCTGATGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTTCCAGCTCATACTAGCTGGTTTAACAATTGTTACGCACATACTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATATTTTTCTGTTGCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAGCCAAAAAGCAGTCGGAACTCGAGTATAGAGGCATTTGCAGTTTGTGAGAACTATTCTCCTCCCGAAGGTTTCAATCCAAAAGATCTGCATCGCTTGCTGGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGATTGCAGTAGTGGATGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATATCCACTGCCCAGAGGTGCTGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATTGCCCCTCCATACAAGAGGGCTCTCGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGA

Coding sequence (CDS)

ATGGACATGATGACTCTTCCTTCGCTTCTTCAATCACCTATTTCGTCTTGGGAGGAGAGATGTGAGAGTCTAGAGCCCCAGTCCTTTGGACGGGTTTTCGTGAGGCCTGTCTGCTCTGCACTACCGGATATATACTACCGTAAAGCAAAAGAAGAAGGTTGGCGTGCTAGAAGTGCATTTAAACTTCTTCAGATAGATGAGGAATTTAACATATTTGAAGGAGTCAAGAGAGTGGTAGATTTATGTGCAGCCCCCGGTAGCTGGAGCCAGGTCCTCAGTAGAAAATTGTATCTCCCAGCAAAGCTTTCTCCAGATTTAAAGGATGGCGATCTTCCTCTTATTGTTGCTATTGATTTACAGCCGATGGCTCCAATTGAAGGTGTTATTGAAGTGCAGGGTGATATAACAAATGCTAGGACAGCTGAAGTGGTCATTAGGCATTTTGATGGGTGCAAGGCTGACCTGGTTGTTTGTGACGGTGCACCTGATGTTACTGGTCTTCATGACATGGACGAGTTTGTTCAGTTCCAGCTCATACTAGCTGGTTTAACAATTGTTACGCACATACTCAGAGAAGGTGGTAAGTTCATAGCAAAGATATTCCGTGGAAAGGATACCAGCCTTTTGTATATTTTTCTGTTGCAGCTGAAGTTATTTTTCCCTGTAGTGACTTTTGCAAAGCCAAAAAGCAGTCGGAACTCGAGTATAGAGGCATTTGCAGTTTGTGAGAACTATTCTCCTCCCGAAGGTTTCAATCCAAAAGATCTGCATCGCTTGCTGGAAAAGGTGGGAAGTCCGTCAGGAGGAGATGACTTAGATTGCAGTAGTGGATGGCTGGAAGGTCCCAATAAAGTTTACATCCCATTTCTTGCCTGTGGGGACCTCAGTGGATATGACTCCGACCGTTCATATCCACTGCCCAGAGGTGCTGAGGGAACTTATCGGAGCTTAGATCCTGTGCAGCCTCCTATTGCCCCTCCATACAAGAGGGCTCTCGAAATGAAGAAAGCTTCCAGTCAAGGAATTCGAGAACTTGAAAAACTTTCCTTGGATTCCTGA

Protein sequence

MDMMTLPSLLQSPISSWEERCESLEPQSFGRVFVRPVCSALPDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGIRELEKLSLDS
Homology
BLAST of IVF0008879 vs. ExPASy Swiss-Prot
Match: Q9UET6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapiens OX=9606 GN=FTSJ1 PE=1 SV=2)

HSP 1 Score: 337.0 bits (863), Expect = 2.5e-91
Identity = 167/294 (56.80%), Postives = 207/294 (70.41%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           D+YYR AKE GWRARSAFKLLQ+D+EF +F+GV R VDLCAAPGSWSQVLS+K       
Sbjct: 10  DVYYRLAKENGWRARSAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVLSQK------- 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
              +       +VA+DLQ MAP+ GV+++QGDIT   TA+ +I+HF GC ADLVVCDGAP
Sbjct: 70  ---IGGQGSGHVVAVDLQAMAPLPGVVQIQGDITQLSTAKEIIQHFKGCPADLVVCDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHD+DE++Q QL+LA L I TH+L+ GG F+AKIFRG+D +LLY    QL++FF  
Sbjct: 130 DVTGLHDVDEYMQAQLLLAALNIATHVLKPGGCFVAKIFRGRDVTLLY---SQLQVFFSS 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           V  AKP+SSRNSSIEAFAVC+ Y PPEGF P     LL+    P            L+GP
Sbjct: 190 VLCAKPRSSRNSSIEAFAVCQGYDPPEGFIPDLSKPLLDHSYDPDFNQ--------LDGP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 337
            ++ +PF+ CGDLS YDSDRSYPL       Y+   P QPPI+PPY+ A  +K+
Sbjct: 250 TRIIVPFVTCGDLSSYDSDRSYPLDLEGGSEYKYTPPTQPPISPPYQEACTLKR 282

BLAST of IVF0008879 vs. ExPASy Swiss-Prot
Match: O36015 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4F10.03c PE=3 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.2e-82
Identity = 159/293 (54.27%), Postives = 206/293 (70.31%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           D YYR AKE+GWRARSAFKLLQ++E+FN+FEG KRVVDLCAAPGSWSQVLSR+L     +
Sbjct: 10  DAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSWSQVLSRELL--KNI 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
              +   + P+IVA+DLQPMAPI+GV  +Q DIT+  T  +++ HF    ADLVV DGAP
Sbjct: 70  DTSIAADEKPMIVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNEPADLVVSDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHD+DE++Q Q++LA   +   +L+ GGKF+AKIFRG+D SLLY    QL+L F  
Sbjct: 130 DVTGLHDLDEYIQAQILLAAFNLAVCVLKPGGKFVAKIFRGRDVSLLY---SQLRLMFRK 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           V+ AKP+SSR SSIE+F VCE+++PP  F P DL + L  V  P+   ++          
Sbjct: 190 VSCAKPRSSRASSIESFVVCEDFNPPSNFQP-DLTKPL-CVIDPTNAHEI---------- 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMK 336
                PF+ACGDL GYD+D +YP+    +    +LD +QPP APPYKRA+E+K
Sbjct: 250 ----APFIACGDLDGYDADATYPVEINMKKA--TLDVIQPPTAPPYKRAIELK 279

BLAST of IVF0008879 vs. ExPASy Swiss-Prot
Match: Q22031 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabditis elegans OX=6239 GN=R74.7 PE=3 SV=3)

HSP 1 Score: 303.9 bits (777), Expect = 2.3e-81
Identity = 163/306 (53.27%), Postives = 205/306 (66.99%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYR AKE  WRARSAFKL+QID+EF I +GV+R VDLCAAPGSWSQVLS++LY     
Sbjct: 10  DIYYRLAKENKWRARSAFKLMQIDDEFQILKGVRRAVDLCAAPGSWSQVLSKRLY----- 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
               ++     IVAIDLQPMAPI GVI++QGDIT+  TA  VI+HF G K+D+V+CDGAP
Sbjct: 70  ----EEDQEAKIVAIDLQPMAPIPGVIQLQGDITSVDTANQVIKHFSGEKSDIVICDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTG+H +DEF+Q +LILA   I +H+L+EGG F+AKIFR +++SLLY    Q+K +F  
Sbjct: 130 DVTGIHSLDEFMQAELILAAFNITSHVLKEGGNFLAKIFRSRNSSLLY---AQMKKYFKK 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           V  AKP+SSR SS EAF +C +YSPPEGF P              G   LD +      P
Sbjct: 190 VYLAKPRSSRQSSCEAFVLCLDYSPPEGFVP------------TMGKTSLDATDASAISP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPL------PRG-----AEGTYRSLDPVQPPIAPPYKRA 338
           + +   F+ CGDLSG+DS++SYPL      P+G      +  Y   D VQPP  P YK A
Sbjct: 250 D-IIDGFVTCGDLSGWDSEKSYPLDIDACFPKGEIDEEQKKRYEFKDVVQPPTDPAYKAA 290

BLAST of IVF0008879 vs. ExPASy Swiss-Prot
Match: Q54VA8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=fsjA PE=3 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 5.1e-76
Identity = 148/268 (55.22%), Postives = 192/268 (71.64%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYRKAKEEGWRARSAFKL+QIDEE+ IFEGVKRVVDLCAAPGSWSQVLSR++Y   K 
Sbjct: 10  DIYYRKAKEEGWRARSAFKLIQIDEEYQIFEGVKRVVDLCAAPGSWSQVLSRRIYGDGK- 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
            PD+K      IVA+DLQ MAP++GV++++GDIT   T++ +I HFDG  ADL++ DGAP
Sbjct: 70  DPDVK------IVAVDLQEMAPLKGVVQIKGDITKYETSKQIISHFDGSLADLIISDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHD+D + Q QLIL+ L I TH L+ GG F+AK+F+G D SL+Y    Q+KLFF  
Sbjct: 130 DVTGLHDIDFYGQSQLILSALNITTHTLKIGGTFVAKMFKGDDMSLMY---SQMKLFFEH 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLE---KVGSPSGGDDLDCSSGWL 282
           V+F KP SSR SS+E F +C NY PP  +NPK +   LE   K+ + +  +  + ++  +
Sbjct: 190 VSFVKPSSSRESSLENFILCRNYQPPLNYNPKIIDPALENHNKILNNNNNNSNNNNNCNI 249

Query: 283 EGPN--------KVYIPFLACGDLS-GY 299
           +  N         + +PF+ACGDL+ GY
Sbjct: 250 DNENDNEFLKTDSLIVPFIACGDLNLGY 267

BLAST of IVF0008879 vs. ExPASy Swiss-Prot
Match: P38238 (tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TRM7 PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 4.4e-72
Identity = 150/302 (49.67%), Postives = 202/302 (66.89%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFE--GVKRVVDLCAAPGSWSQVLSRKLYLPA 102
           D+YYRKAKE+G+RARSAFKLLQ++++F+  +   +KRVVDLCAAPGSWSQVLSRKL+  +
Sbjct: 10  DLYYRKAKEQGYRARSAFKLLQLNDQFHFLDDPNLKRVVDLCAAPGSWSQVLSRKLFDES 69

Query: 103 KLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDG 162
             S    D +   IV++DLQPM+PI  V  +Q DIT+ +T   +++ F   KAD V  DG
Sbjct: 70  PSS----DKEDRKIVSVDLQPMSPIPHVTTLQADITHPKTLARILKLFGNEKADFVCSDG 129

Query: 163 APDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFF 222
           APDVTGLHD+DE+VQ QLI++ L +   IL++GG F+AKIFRG+D  +LY    QL   F
Sbjct: 130 APDVTGLHDLDEYVQQQLIMSALQLTACILKKGGTFVAKIFRGRDIDMLY---SQLGYLF 189

Query: 223 PVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPK-DLHRLLEKVGSPSGGDDL---DCSS 282
             +  AKP+SSR +S+EAF VC  Y+PP  + PK D++  +++       + L   D  S
Sbjct: 190 DKIVCAKPRSSRGTSLEAFIVCLGYNPPSNWTPKLDVNTSVDEFFQGCFLNKLCISDKLS 249

Query: 283 GWLEGPNKVYIPFLACGDLSGYDSDRSY-PLPRGAEGTYRSLDPVQPPIAPPYKRALEMK 338
            W E    +   F+ACG L  +DSD +Y  LP    GT  SLDPVQ P  PPYK+ALE+K
Sbjct: 250 HWNEEERNI-AEFMACGSLQSFDSDATYHDLPSSVAGTSSSLDPVQSPTNPPYKKALELK 303

BLAST of IVF0008879 vs. ExPASy TrEMBL
Match: A0A5A7TQN9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00950 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.3e-194
Identity = 348/379 (91.82%), Postives = 348/379 (91.82%), Query Frame = 0

Query: 1   MDMMTLPSLLQSPISSWEERCESLEPQSFGRVFV-------------------------- 60
           MDMMTLPSLLQSPISSWEERCESLEPQSFGRVFV                          
Sbjct: 1   MDMMTLPSLLQSPISSWEERCESLEPQSFGRVFVVRHRIVKDMIEDFLLNPPFGKKDRFL 60

Query: 61  -RPVCSALPDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 120
            RPVCSALPDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 61  WRPVCSALPDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 120

Query: 121 RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 180
           RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA
Sbjct: 121 RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 180

Query: 181 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFL 240
           DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLY   
Sbjct: 181 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLY--- 240

Query: 241 LQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLD 300
            QLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLD
Sbjct: 241 SQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLD 300

Query: 301 CSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALE 353
           CSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALE
Sbjct: 301 CSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALE 360

BLAST of IVF0008879 vs. ExPASy TrEMBL
Match: A0A1S3B6C8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 2.7e-173
Identity = 306/310 (98.71%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
           SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLY    QLKLFFPV
Sbjct: 130 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLY---SQLKLFFPV 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP
Sbjct: 190 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 342
           NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI
Sbjct: 250 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 309

Query: 343 RELEKLSLDS 353
           RELEKLSLDS
Sbjct: 310 RELEKLSLDS 316

BLAST of IVF0008879 vs. ExPASy TrEMBL
Match: A0A0A0LCV6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G732420 PE=3 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.7e-172
Identity = 304/310 (98.06%), Postives = 305/310 (98.39%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
           SPDLKDG+LPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLY    QLKLFFPV
Sbjct: 130 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLY---SQLKLFFPV 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           VTFAKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSSGWLEGP
Sbjct: 190 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 342
           NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI
Sbjct: 250 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 309

Query: 343 RELEKLSLDS 353
           RELEKLSLDS
Sbjct: 310 RELEKLSLDS 316

BLAST of IVF0008879 vs. ExPASy TrEMBL
Match: A0A6J1F1P7 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111441398 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.8e-169
Identity = 299/310 (96.45%), Postives = 302/310 (97.42%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
           SPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY    QLKLFFPV
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLY---CQLKLFFPV 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP
Sbjct: 190 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 342
           NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI
Sbjct: 250 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 309

Query: 343 RELEKLSLDS 353
           RELEKLS DS
Sbjct: 310 RELEKLSSDS 316

BLAST of IVF0008879 vs. ExPASy TrEMBL
Match: A0A6J1IYY9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111481931 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.3e-167
Identity = 297/310 (95.81%), Postives = 300/310 (96.77%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYRKAKEEGWRARSAFKLLQID+EFNI EGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDDEFNILEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
           SPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY    QLKLFFPV
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLY---CQLKLFFPV 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           VTFAKPKSSRNSSIEAFAVCENYSP EGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP
Sbjct: 190 VTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 342
           NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI
Sbjct: 250 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 309

Query: 343 RELEKLSLDS 353
           RELEKLS DS
Sbjct: 310 RELEKLSSDS 316

BLAST of IVF0008879 vs. NCBI nr
Match: KAA0044157.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo var. makuwa] >TYK24978.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 686 bits (1769), Expect = 4.39e-248
Identity = 348/379 (91.82%), Postives = 348/379 (91.82%), Query Frame = 0

Query: 1   MDMMTLPSLLQSPISSWEERCESLEPQSFGRVFV-------------------------- 60
           MDMMTLPSLLQSPISSWEERCESLEPQSFGRVFV                          
Sbjct: 1   MDMMTLPSLLQSPISSWEERCESLEPQSFGRVFVVRHRIVKDMIEDFLLNPPFGKKDRFL 60

Query: 61  -RPVCSALPDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 120
            RPVCSALPDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS
Sbjct: 61  WRPVCSALPDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLS 120

Query: 121 RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 180
           RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA
Sbjct: 121 RKLYLPAKLSPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKA 180

Query: 181 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFL 240
           DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLY   
Sbjct: 181 DLVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYS-- 240

Query: 241 LQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLD 300
            QLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLD
Sbjct: 241 -QLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLD 300

Query: 301 CSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALE 352
           CSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALE
Sbjct: 301 CSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALE 360

BLAST of IVF0008879 vs. NCBI nr
Match: XP_008442455.1 (PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442456.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442457.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 615 bits (1586), Expect = 3.62e-221
Identity = 306/310 (98.71%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
           SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLY    QLKLFFPV
Sbjct: 130 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYS---QLKLFFPV 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP
Sbjct: 190 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 342
           NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI
Sbjct: 250 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 309

Query: 343 RELEKLSLDS 352
           RELEKLSLDS
Sbjct: 310 RELEKLSLDS 316

BLAST of IVF0008879 vs. NCBI nr
Match: XP_004137730.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >XP_011651872.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >XP_031738324.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >KGN58784.1 hypothetical protein Csa_000714 [Cucumis sativus])

HSP 1 Score: 610 bits (1574), Expect = 2.44e-219
Identity = 304/310 (98.06%), Postives = 305/310 (98.39%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
           SPDLKDG+LPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDLKDGELPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLY    QLKLFFPV
Sbjct: 130 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYS---QLKLFFPV 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           VTFAKPKSSRNSSIEAFAVCENYSPPEGFN KDLHRLLEKVGSPSGGDDLDCSSGWLEGP
Sbjct: 190 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 342
           NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI
Sbjct: 250 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 309

Query: 343 RELEKLSLDS 352
           RELEKLSLDS
Sbjct: 310 RELEKLSLDS 316

BLAST of IVF0008879 vs. NCBI nr
Match: XP_038903231.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa hispida])

HSP 1 Score: 603 bits (1555), Expect = 1.92e-216
Identity = 299/310 (96.45%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGV+RVVDLCAAPGSWSQVLSRKL+LPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVERVVDLCAAPGSWSQVLSRKLHLPAKL 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
           SPD KDGDLPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDSKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY    QLKLFFPV
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYC---QLKLFFPV 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP
Sbjct: 190 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 342
           NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYR LDPVQPPIAPPYKRALEMKKASSQGI
Sbjct: 250 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRCLDPVQPPIAPPYKRALEMKKASSQGI 309

Query: 343 RELEKLSLDS 352
           RELEKLSLDS
Sbjct: 310 RELEKLSLDS 316

BLAST of IVF0008879 vs. NCBI nr
Match: XP_022934133.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_022934134.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_023528668.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >XP_023528669.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >KAG7017237.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 602 bits (1553), Expect = 3.86e-216
Identity = 299/310 (96.45%), Postives = 302/310 (97.42%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYRKAKEEGWRARSAFKLLQID+EFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDDEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
           SPD KDGD PLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SPDSKDGDHPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHDMDEFVQ QLILAGLTIVTH+LREGGKFIAKIFRGKDTSLLY    QLKLFFPV
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHVLREGGKFIAKIFRGKDTSLLYC---QLKLFFPV 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP
Sbjct: 190 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 342
           NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI
Sbjct: 250 NKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKKASSQGI 309

Query: 343 RELEKLSLDS 352
           RELEKLS DS
Sbjct: 310 RELEKLSSDS 316

BLAST of IVF0008879 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 560.1 bits (1442), Expect = 1.3e-159
Identity = 275/299 (91.97%), Postives = 287/299 (95.99%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSR+LYLPAK 
Sbjct: 10  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRQLYLPAKS 69

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 162
           S + KDGDLPLIVAIDLQPMAPIEGVI+VQGDITNARTAEVVIRHFDGCKADLVVCDGAP
Sbjct: 70  SAESKDGDLPLIVAIDLQPMAPIEGVIQVQGDITNARTAEVVIRHFDGCKADLVVCDGAP 129

Query: 163 DVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKDTSLLYIFLLQLKLFFPV 222
           DVTGLHDMDEFVQ QLILAGLTIVTHIL+EGGKFIAKIFRGKDTSLLY    QLKLFFP 
Sbjct: 130 DVTGLHDMDEFVQSQLILAGLTIVTHILKEGGKFIAKIFRGKDTSLLY---CQLKLFFPT 189

Query: 223 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWLEGP 282
           VTFAKPKSSRNSSIEAFAVCENYSPPEGFNP+DLHRLLEKVGSPSGG DLDCSSGWLEGP
Sbjct: 190 VTFAKPKSSRNSSIEAFAVCENYSPPEGFNPRDLHRLLEKVGSPSGGSDLDCSSGWLEGP 249

Query: 283 NKVYIPFLACGDLSGYDSDRSYPLPRGAEG-TYRSLDPVQPPIAPPYKRALEMKKASSQ 341
           NKVYIPFLACGDL+GYDSDRSYPLPR A+G +Y+SLDP+QPPIAPPYKRALE+KKAS+Q
Sbjct: 250 NKVYIPFLACGDLTGYDSDRSYPLPREADGSSYQSLDPIQPPIAPPYKRALELKKASAQ 305

BLAST of IVF0008879 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 122.9 bits (307), Expect = 5.3e-28
Identity = 76/233 (32.62%), Postives = 123/233 (52.79%), Query Frame = 0

Query: 43  DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYLPAKL 102
           D YYR AKE G+R+R+++KLLQ+D ++++      V+DLCAAPG W QV   K+ + +  
Sbjct: 11  DKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQVAVEKVPVGS-- 70

Query: 103 SPDLKDGDLPLIVAIDLQPMAPIEGVIEVQGDIT----NARTAEVVIRHFDGCKA-DLVV 162
                     L++ IDL P+ P+ G + +  DIT     ++  +V+ +H  G  A +LV+
Sbjct: 71  ----------LVLGIDLVPILPVRGCVTMTQDITRTECKSKIKQVMEQH--GVSAFNLVL 130

Query: 163 CDGAPDVTGLHDMDEFVQFQLILAGLTIVTHILREGGKFIAKIFRGKD-TSLLYIFLLQL 222
            DG+P+V G    +   Q  L++  + + T  L   G  + K+FR +D  S+LY     L
Sbjct: 131 HDGSPNVGGAWAQEAMSQNALVIDSVRLATEFLARNGNLVTKVFRSRDYNSVLYC----L 190

Query: 223 KLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPK--DLHRLLEKVGSPS 268
              F  V   KP +SR++S E + V   Y  P   +P+  D   L ++   P+
Sbjct: 191 GRLFEKVEVFKPPASRSASAETYLVGLKYLAPAKIDPRLLDYRHLFKESAEPT 225

BLAST of IVF0008879 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 1.5e-22
Identity = 48/48 (100.00%), Postives = 48/48 (100.00%), Query Frame = 0

Query: 43 DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 91
          DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ
Sbjct: 10 DIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57

BLAST of IVF0008879 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 74.7 bits (182), Expect = 1.6e-13
Identity = 56/232 (24.14%), Postives = 102/232 (43.97%), Query Frame = 0

Query: 39  SALPDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVLSRKLYL 98
           + +PD +YR+A+  G+ ARSAFKLLQI +++ + +    V+DL  APG+W QV  + L  
Sbjct: 4   AGVPDFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQSL-- 63

Query: 99  PAKLSPDLKDGDLPLIVAIDLQ----PMAPIEGVIEVQGDITNARTAEVVIRHFDGCKAD 158
                  L+ G   ++V +D++    P      V  +  D+ N    ++           
Sbjct: 64  -----GPLRSGG--IVVGMDIKKVKVPPQCDSRVQTIAADVLNFPRQKIRELSPQQLGFS 123

Query: 159 LVVCDGAPDVTGLHDMDEFVQFQLILAGLTIVT---------------------HILREG 218
           +++ D    V+G+   D  +  +L +  L +                        +LR G
Sbjct: 124 VILSDMCHSVSGITTRDAALSAELGMRALDLAVGQAAISQSPNDDDGGPNESRPGVLRHG 183

Query: 219 GKFIAKIFRGKDTSLLYIFLLQLKLFFPVVTFAKPKSSRNSSIEAFAVCENY 246
           G  + K+   +D      F    K  F   ++ +PK++R SS E + +C+ +
Sbjct: 184 GHLVIKLLESEDAQ---DFARICKPIFNKASWLRPKATRPSSREIYLICQGF 223

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9UET62.5e-9156.80Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Homo sapien... [more]
O360151.2e-8254.27Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosacch... [more]
Q220312.3e-8153.27Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Caenorhabdi... [more]
Q54VA85.1e-7655.22Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Dictyosteli... [more]
P382384.4e-7249.67tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Saccharomyces cerevi... [more]
Match NameE-valueIdentityDescription
A0A5A7TQN91.3e-19491.82Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel... [more]
A0A1S3B6C82.7e-17398.71Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel... [more]
A0A0A0LCV66.7e-17298.06Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sat... [more]
A0A6J1F1P71.8e-16996.45Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m... [more]
A0A6J1IYY91.3e-16795.81Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m... [more]
Match NameE-valueIdentityDescription
KAA0044157.14.39e-24891.82putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cu... [more]
XP_008442455.13.62e-22198.71PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase iso... [more]
XP_004137730.12.44e-21998.06putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativ... [more]
XP_038903231.11.92e-21696.45putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa his... [more]
XP_022934133.13.86e-21696.45putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita mos... [more]
Match NameE-valueIdentityDescription
AT5G01230.11.3e-15991.97S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G25730.15.3e-2832.62FtsJ-like methyltransferase family protein [more]
AT5G01230.21.5e-22100.00S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G13830.11.6e-1324.14FtsJ-like methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 53..251
e-value: 4.1E-70
score: 237.5
NoneNo IPR availablePANTHERPTHR10920RIBOSOMAL RNA METHYLTRANSFERASEcoord: 43..344
NoneNo IPR availablePANTHERPTHR10920:SF25TRNA (CYTIDINE(32)/GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATEDcoord: 43..344
IPR002877Ribosomal RNA methyltransferase FtsJ domainPFAMPF01728FtsJcoord: 54..245
e-value: 4.0E-61
score: 206.2
IPR028590tRNA (cytidine(32)/guanosine(34)-2-O)-methyltransferase, Trm7HAMAPMF_03162RNA_methyltr_E_TRM7coord: 34..304
score: 30.96648
IPR015507Ribosomal RNA large subunit methyltransferase EHAMAPMF_01547RNA_methyltr_Ecoord: 38..246
score: 30.438572
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 53..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0008879.1IVF0008879.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002181 cytoplasmic translation
biological_process GO:0002128 tRNA nucleoside ribose methylation
biological_process GO:0032259 methylation
biological_process GO:0001510 RNA methylation
biological_process GO:0008033 tRNA processing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008175 tRNA methyltransferase activity
molecular_function GO:0008168 methyltransferase activity