IVF0001299 (gene) Melon (IVF77) v1

BLAST of IVF0001299 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 6.5e-126
Identity = 273/450 (60.67%), Postives = 302/450 (67.11%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLDD---------------------- 60
           MAT N  TG+ +    ++   DRL+S + V D F  D                       
Sbjct: 1   MATMNLPTGLHVAAKPAS--LDRLSSAKNVGDLFFSDSRHRKRVNTSNQIMAVQSLEHIH 60

Query: 61  -VSNS------TFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMN 120
            V+N+       F + S+ +   +  +   S RKTKIVCTIGPSTSSREMIWKLAE GMN
Sbjct: 61  GVNNNVYANYVNFNVPSSGYSLGQESVYLNSPRKTKIVCTIGPSTSSREMIWKLAEAGMN 120

Query: 121 VARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQE 180
           VARLNMSHGDH+SHQ+TIDLVKEYNAQF DKVIAIMLDTKGPEV SGDVPKPILLKEGQE
Sbjct: 121 VARLNMSHGDHASHQRTIDLVKEYNAQFEDKVIAIMLDTKGPEVISGDVPKPILLKEGQE 180

Query: 181 FNFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGE 240
           FNF+I+RGVSTEDTVSVNYDDF+NDVE GD LLVDGGMMSLAV+SKT D VKC VIDGGE
Sbjct: 181 FNFSIKRGVSTEDTVSVNYDDFINDVEAGDILLVDGGMMSLAVKSKTSDIVKCEVIDGGE 240

Query: 241 LKSRRHLNVRGKSATF-----LGW-IIRL-------IYAVSFVKDARVVHELKDYLKSTF 300
           LKSRRHLNVRGKSAT        W  I+         YAVSFVKDA+VVHELKDYLK   
Sbjct: 241 LKSRRHLNVRGKSATLPSITEKDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYLK--- 300

Query: 301 LYSCDC------KIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAELPIEEVPLL 360
             SC+       KIESADSIPNLH       G        AMVARGDLGAELPIEEVPLL
Sbjct: 301 --SCNADIHVIVKIESADSIPNLHSIISASDG--------AMVARGDLGAELPIEEVPLL 360

Query: 361 QVKTLTHHITVTFLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVSDIAIAVREG 403
           Q                 I+RC+SMQK VIVATNMLESMIDHPTPTRAEVSDI+IAVREG
Sbjct: 361 Q--------------EDIIRRCQSMQKPVIVATNMLESMIDHPTPTRAEVSDISIAVREG 416

BLAST of IVF0001299 vs. ExPASy Swiss-Prot
Match: Q93Z53 (Plastidial pyruvate kinase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP3 PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 1.2e-116
Identity = 263/457 (57.55%), Postives = 306/457 (66.96%), Query Frame = 0

Query: 33  AFGLDDVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNV 92
           AF +  + +S++ L       SRT  +  SRRKTKIVCTIGPS+SSREMIWKLAE GMNV
Sbjct: 74  AFDVGVLDSSSYRLAD-----SRTS-SNDSRRKTKIVCTIGPSSSSREMIWKLAEAGMNV 133

Query: 93  ARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEF 152
           ARLNMSHGDH+SHQ TIDLVKEYN+ F DK IAIMLDTKGPEVRSGDVP+PI L+EGQEF
Sbjct: 134 ARLNMSHGDHASHQITIDLVKEYNSLFVDKAIAIMLDTKGPEVRSGDVPQPIFLEEGQEF 193

Query: 153 NFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGEL 212
           NFTI+RGVS +DTVSVNYDDFVNDVEVGD LLVDGGMMSLAV+SKT D VKCVVIDGGEL
Sbjct: 194 NFTIKRGVSLKDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGEL 253

Query: 213 KSRRHLNVRGKSATF-----LGW-IIRL-------IYAVSFVKDARVVHELKDYLKSTFL 272
           +SRRHLNVRGKSAT        W  I+         YAVSFVKDA+VVHELK+YLK+   
Sbjct: 254 QSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSA 313

Query: 273 -YSCDCKIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAELPIEEVPLLQVKTLT 332
             S   KIESADSI NL        G        AMVARGDLGAELPIEEVPLLQ +   
Sbjct: 314 DISVIVKIESADSIKNLPSIISACDG--------AMVARGDLGAELPIEEVPLLQEE--- 373

Query: 333 HHITVTFLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVSF 392
                       I+RCRS+ K VIVATNMLESMI+HPTPTRAEVSDIAIAVREGADA+  
Sbjct: 374 -----------IIRRCRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAI-- 433

Query: 393 QEKLLMGSTFLAHYLGRRQKNIH-LFKRNGSTVHMNTKINVWNYIVDHLLYSIFFKTSFL 452
              +L G T    +  +    +H +  R  +++ + T  +       H+     F  S +
Sbjct: 434 ---MLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTAYKGHMGQMFAFHASIM 493

Query: 453 -QIICGYFLYLSRKGRVGMYKSHLYGRPTIIV-TDQK 473
              +    +  +R G + +  SH     TI   T+Q+
Sbjct: 494 ANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQR 497

BLAST of IVF0001299 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.6e-111
Identity = 226/328 (68.90%), Postives = 245/328 (74.70%), Query Frame = 0

Query: 63  RRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDK 122
           RRKTKIVCT+GPST++REMIWKLAE GMNVAR+NMSHGDH+SH+K IDLVKEYNAQ  D 
Sbjct: 109 RRKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDN 168

Query: 123 VIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVNDVEVGDT 182
            IAIMLDTKGPEVRSGD+P+PI+L  GQEF FTI RGVST   VSVNYDDFVNDVE GD 
Sbjct: 169 TIAIMLDTKGPEVRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVNDVEAGDM 228

Query: 183 LLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGKSATF------------LGW 242
           LLVDGGMMS  V+SKT DSVKC V+DGGELKSRRHLNVRGKSAT              G 
Sbjct: 229 LLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGV 288

Query: 243 IIRL-IYAVSFVKDARVVHELKDYLKSTFL-YSCDCKIESADSIPNLHRYFQHQMGLAFY 302
             ++  YAVSFVKDA+VVHELK YL+++        KIESADSIPNLH       G    
Sbjct: 289 ENKVDFYAVSFVKDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITASDG---- 348

Query: 303 TIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQKTVIVATNML 362
               AMVARGDLGAELPIEEVP+LQ +               I  CRSM K VIVATNML
Sbjct: 349 ----AMVARGDLGAELPIEEVPILQEE--------------IINLCRSMGKAVIVATNML 408

Query: 363 ESMIDHPTPTRAEVSDIAIAVREGADAV 377
           ESMI HPTPTRAEVSDIAIAVREGADAV
Sbjct: 409 ESMIVHPTPTRAEVSDIAIAVREGADAV 414

BLAST of IVF0001299 vs. ExPASy Swiss-Prot
Match: P55964 (Pyruvate kinase isozyme G, chloroplastic (Fragment) OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 3.5e-87
Identity = 210/372 (56.45%), Postives = 237/372 (63.71%), Query Frame = 0

Query: 116 NAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVN 175
           NAQ +D V++IMLDTKGPEVRSGDVP+P +LKEGQEFN TIRRGVST+DTVSVNYDDFVN
Sbjct: 1   NAQSHDNVVSIMLDTKGPEVRSGDVPQP-MLKEGQEFNPTIRRGVSTQDTVSVNYDDFVN 60

Query: 176 DVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGKSA-----TFLGW 235
           DV VGD LLVDGGMMSLAV+SKT D VKCVV+DGGELKSRRHLNVRGKSA     T   W
Sbjct: 61  DVVVGDILLVDGGMMSLAVKSKTSDLVKCVVVDGGELKSRRHLNVRGKSARLPSITDKDW 120

Query: 236 -IIRL-------IYAVSFVKDARVVHELKDYLKSTFLYSCDC------KIESADSIPNLH 295
             I+         YAVSFVKDA+VVHELK+YLK      C+       KIESADSIPNLH
Sbjct: 121 GDIKFGVDNQVDFYAVSFVKDAKVVHELKEYLK-----RCNADIHVIVKIESADSIPNLH 180

Query: 296 RYFQHQMGLAFYTIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRS 355
                  G        AMVARGDLGAELPIEEVPLLQ                 I+RC S
Sbjct: 181 SIISASDG--------AMVARGDLGAELPIEEVPLLQ--------------EDIIRRCHS 240

Query: 356 MQKTVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVSFQEKLLMGSTFLAHYLGRR 415
           MQK VIVATNMLESMI+HPTPTRAEVSDIAIAVREGADAV     +L G T    Y  + 
Sbjct: 241 MQKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAV-----MLSGETAHGKYPLKA 300

Query: 416 QKNIH-LFKRNGSTVHMNTKINVWNYIVDHL-LYSIFFKTSFLQIICGYFLYLSRKGRVG 467
            + +H +  R  S+  +NT          H+     F  T     +    +  +R G + 
Sbjct: 301 VRVMHTVALRTESSSPVNTTPPAQGAYKGHMGEMFAFHATIMANTLNTPIIVFTRTGSMA 339

BLAST of IVF0001299 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.9e-54
Identity = 151/399 (37.84%), Postives = 215/399 (53.88%), Query Frame = 0

Query: 15  SDSTRVADRLASCRIVSDAFGLDDVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGP 74
           S +T+   R A+    S +  +D V+ +  EL+ N F         +S R+TK++CTIGP
Sbjct: 76  SSATQEYGRNAAPGTDSSSIEVDTVTEA--ELKENGF---------RSTRRTKLICTIGP 135

Query: 75  STSSREMIWKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPE 134
           +T   E + +LAE GMNVAR+NM HG    H+  I+ ++  N +     +AIM+DT+G E
Sbjct: 136 ATCGFEQLERLAEGGMNVARINMCHGTREWHRMVIERLRRLNEE-KGFAVAIMMDTEGSE 195

Query: 135 VRSGDV--PKPILLKEGQEFNFTIRR--GVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMM 194
           +  GD+        ++G+ +NFT+R       E TV+VNYD F  DV+VGD LLVDGGM+
Sbjct: 196 IHMGDLGGASSAKAEDGEIWNFTVRSFDPPLPERTVTVNYDGFAEDVKVGDELLVDGGMV 255

Query: 195 SLAVQSKTDDSVKCVVIDGGELKSRRHLN-------VRGKSA-----TFLGWI------- 254
              V  K    VKC+  D G L  R +L        VR ++A     +   W+       
Sbjct: 256 RFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGKLVRERNAMLPTISSKDWLDIDFGIA 315

Query: 255 --IRLIYAVSFVKDARVVHELKDYLKSTFL---YSCDCKIESADSIPNLHRYFQHQMGLA 314
             +  I AVSFVK A V+  LK Y+++       S   KIES DS+ NL    Q   G  
Sbjct: 316 EGVDFI-AVSFVKSAEVIKHLKSYIQARARDSDISVIAKIESIDSLKNLEEIIQASDG-- 375

Query: 315 FYTIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQKTVIVATN 374
                 AMVARGDLGA++P+E+VP  Q K               ++ CR + + VIVA+ 
Sbjct: 376 ------AMVARGDLGAQIPLEQVPSEQQK--------------IVQICRQLNRPVIVASQ 435

Query: 375 MLESMIDHPTPTRAEVSDIAIAVREGADAVSFQEKLLMG 386
           +LESMI++P PTRAEV+D++ AVR+  DA+    +  MG
Sbjct: 436 LLESMIEYPIPTRAEVADVSEAVRQRGDALMLSGESAMG 439

BLAST of IVF0001299 vs. ExPASy TrEMBL
Match: A0A5D3E4W6 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004170 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 1.2e-162
Identity = 335/437 (76.66%), Postives = 340/437 (77.80%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD-----------------DVSNST 60
           MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD                 DVSNST
Sbjct: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLDVKSGKMCLQSKQIALDQDVSNST 60

Query: 61  FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS 120
           FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS
Sbjct: 61  FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS 120

Query: 121 SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTE 180
           SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTE
Sbjct: 121 SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTE 180

Query: 181 DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK 240
           DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK
Sbjct: 181 DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK 240

Query: 241 SATF-----LGW-IIRL-------IYAVSFVKDARVVHELKDYLKSTFLYSCDC------ 300
           SAT        W  I+         YAVSFVKDARVVHELKDYLK     SC+       
Sbjct: 241 SATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHELKDYLK-----SCNADIHVIV 300

Query: 301 KIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVT 360
           KIESADSIPNLH       G        AMVARGDLGAELPIEEVPLLQ           
Sbjct: 301 KIESADSIPNLHSILSASDG--------AMVARGDLGAELPIEEVPLLQ----------- 360

Query: 361 FLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVSFQEKLLM 402
                 IKRCRSMQK VIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAV     +L 
Sbjct: 361 ---EDIIKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAV-----MLS 405

BLAST of IVF0001299 vs. ExPASy TrEMBL
Match: A0A5A7T5H5 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00670 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 2.6e-162
Identity = 334/437 (76.43%), Postives = 340/437 (77.80%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD-----------------DVSNST 60
           MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD                 DVSNST
Sbjct: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLDVKSGKMCLQSKQIALDQDVSNST 60

Query: 61  FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS 120
           FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS
Sbjct: 61  FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS 120

Query: 121 SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTE 180
           SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTI+RGVSTE
Sbjct: 121 SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIKRGVSTE 180

Query: 181 DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK 240
           DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK
Sbjct: 181 DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK 240

Query: 241 SATF-----LGW-IIRL-------IYAVSFVKDARVVHELKDYLKSTFLYSCDC------ 300
           SAT        W  I+         YAVSFVKDARVVHELKDYLK     SC+       
Sbjct: 241 SATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHELKDYLK-----SCNADIHVIV 300

Query: 301 KIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVT 360
           KIESADSIPNLH       G        AMVARGDLGAELPIEEVPLLQ           
Sbjct: 301 KIESADSIPNLHSILSASDG--------AMVARGDLGAELPIEEVPLLQ----------- 360

Query: 361 FLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVSFQEKLLM 402
                 IKRCRSMQK VIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAV     +L 
Sbjct: 361 ---EDIIKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAV-----MLS 405

BLAST of IVF0001299 vs. ExPASy TrEMBL
Match: A0A0A0LHC9 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_3G904080 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 4.1e-155
Identity = 326/458 (71.18%), Postives = 336/458 (73.36%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD----------------------- 60
           MATFN STGVPLLKSDSTR+ADRLASC IVSDAFGL+                       
Sbjct: 1   MATFNLSTGVPLLKSDSTRLADRLASCIIVSDAFGLEVKSGNMCLQSKQIGLVRCLRIVE 60

Query: 61  ---------------DVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
                          DVSNST ELQSNAFH SRTKLTTKSRRKTK+VCTIGPSTSSREMI
Sbjct: 61  QREVASYNGSLDTDQDVSNSTLELQSNAFHRSRTKLTTKSRRKTKVVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNFTI+RGVST+DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV
Sbjct: 181 PILLKEGQEFNFTIKRGVSTKDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATF-----LGW-IIRL-------IYAVSFVKDARVVHE 300
           KCVVIDGGELKSRRHLNVRGKSAT        W  I+         YAVSFVKDARVVHE
Sbjct: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSTFLYSCDC------KIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAE 360
           LKDYLK     SC+       KIESADSIPNLH       G        AMVARGDLGAE
Sbjct: 301 LKDYLK-----SCNADIRVIVKIESADSIPNLHSILSASDG--------AMVARGDLGAE 360

Query: 361 LPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVS 402
           LPIEEVPLLQ                 IKRCRSMQK VIVATNMLESMIDHPTPTRAEVS
Sbjct: 361 LPIEEVPLLQ--------------EDIIKRCRSMQKPVIVATNMLESMIDHPTPTRAEVS 420

BLAST of IVF0001299 vs. ExPASy TrEMBL
Match: A0A6J1DQ72 (Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111023306 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 4.1e-147
Identity = 312/453 (68.87%), Postives = 327/453 (72.19%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD----------------------- 60
           MATFN STGVP+L+SDS RV+DRLASCR V+DAF L+                       
Sbjct: 1   MATFNLSTGVPILRSDSARVSDRLASCRTVADAFALEPKNGRMCLQSKISPIRCRSIAEQ 60

Query: 61  --------------DVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIW 120
                         DVSNS  +LQSN F  SRTKLTT SRRKTKIVCTIGPSTSSREMIW
Sbjct: 61  REVETCDGSLDTDQDVSNSPIKLQSNGFPLSRTKLTTNSRRKTKIVCTIGPSTSSREMIW 120

Query: 121 KLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKP 180
           KLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQF DKVIAIMLDTKGPEVRSGDVP+P
Sbjct: 121 KLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFKDKVIAIMLDTKGPEVRSGDVPQP 180

Query: 181 ILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVK 240
           ILLKEGQEFNFTI+RGVSTEDTVSVNYDDFVNDVEVGD LLVDGGMMSLAV+SKT D VK
Sbjct: 181 ILLKEGQEFNFTIKRGVSTEDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTKDLVK 240

Query: 241 CVVIDGGELKSRRHLNVRGKSATF-----LGW-IIRL-------IYAVSFVKDARVVHEL 300
           CVVIDGGELKSRRHLNVRGKSAT        W  I+         YAVSFVKDARVVHEL
Sbjct: 241 CVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHEL 300

Query: 301 KDYLKS--TFLYSCDCKIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAELPIEE 360
           KDYLKS    +Y    KIESADSIPNLH       G        AMVARGDLGAELPIEE
Sbjct: 301 KDYLKSCNADIYVI-VKIESADSIPNLHSILSASDG--------AMVARGDLGAELPIEE 360

Query: 361 VPLLQVKTLTHHITVTFLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVSDIAIA 402
           VPLLQ +               IKRCRSMQK VIVATNMLESMIDHPTPTRAEVSDIAIA
Sbjct: 361 VPLLQEE--------------IIKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIA 420

BLAST of IVF0001299 vs. ExPASy TrEMBL
Match: A0A6J1GMS3 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111455395 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 2.2e-145
Identity = 305/449 (67.93%), Postives = 326/449 (72.61%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD----------------------- 60
           MATF  STGVPLLKSDSTRVAD LASCR VSDAFG++                       
Sbjct: 1   MATFYLSTGVPLLKSDSTRVADHLASCRNVSDAFGIEAKTGRMYVQSKQIALIRCSRIAE 60

Query: 61  ---------------DVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
                          D+SNST + QSN F  SR KLTTKSRRKTKIVCTIGPSTSSREMI
Sbjct: 61  KQDVASYKGSLDTDQDISNSTLDHQSNGFPLSRMKLTTKSRRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAE GMNVARLNMSHGDHS+HQKTIDLVKEYNAQF DKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAEMGMNVARLNMSHGDHSTHQKTIDLVKEYNAQFKDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLK+GQEFNFTI+RGVSTEDTVSVNYDDF+NDVEVGD++LVDGGMMSL V+SKT+D V
Sbjct: 181 PILLKDGQEFNFTIKRGVSTEDTVSVNYDDFINDVEVGDSVLVDGGMMSLTVKSKTNDLV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATF-----LGW-IIRL-------IYAVSFVKDARVVHE 300
           KCVV+DGGELKSRRHLNVRGKSAT        W  I+         YAVSFVKDARVVHE
Sbjct: 241 KCVVVDGGELKSRRHLNVRGKSATLPSITDKDWDDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSTFLYSCDC------KIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAE 360
           LKDYLK     SC+       KIESADSIPNLH       G        AMVARGDLGAE
Sbjct: 301 LKDYLK-----SCNADIHVIVKIESADSIPNLHSILSASDG--------AMVARGDLGAE 360

Query: 361 LPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVS 393
           LPIEEVPLLQ +T+             IKRC+SMQKTVIVATNMLESMIDHPTPTRAEVS
Sbjct: 361 LPIEEVPLLQ-ETI-------------IKRCQSMQKTVIVATNMLESMIDHPTPTRAEVS 417

BLAST of IVF0001299 vs. NCBI nr
Match: TYK31137.1 (pyruvate kinase isozyme G [Cucumis melo var. makuwa])

HSP 1 Score: 583 bits (1502), Expect = 3.36e-201
Identity = 338/451 (74.94%), Postives = 347/451 (76.94%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD-----------------DVSNST 60
           MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD                 DVSNST
Sbjct: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLDVKSGKMCLQSKQIALDQDVSNST 60

Query: 61  FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS 120
           FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS
Sbjct: 61  FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS 120

Query: 121 SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTE 180
           SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTE
Sbjct: 121 SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTE 180

Query: 181 DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK 240
           DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK
Sbjct: 181 DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK 240

Query: 241 SATFLG-----WI-IRL-------IYAVSFVKDARVVHELKDYLKSTFLYSCDC------ 300
           SAT        W  I+         YAVSFVKDARVVHELKDYLKS     C+       
Sbjct: 241 SATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHELKDYLKS-----CNADIHVIV 300

Query: 301 KIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVT 360
           KIESADSIPNLH       G        AMVARGDLGAELPIEEVPLLQ           
Sbjct: 301 KIESADSIPNLHSILSASDG--------AMVARGDLGAELPIEEVPLLQ----------- 360

Query: 361 FLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVSFQEKLLM 414
                 IKRCRSMQK VIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAV     +L 
Sbjct: 361 ---EDIIKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAV-----MLS 419

BLAST of IVF0001299 vs. NCBI nr
Match: KAA0038540.1 (pyruvate kinase isozyme G [Cucumis melo var. makuwa])

HSP 1 Score: 582 bits (1499), Expect = 9.57e-201
Identity = 337/451 (74.72%), Postives = 347/451 (76.94%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD-----------------DVSNST 60
           MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD                 DVSNST
Sbjct: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLDVKSGKMCLQSKQIALDQDVSNST 60

Query: 61  FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS 120
           FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS
Sbjct: 61  FELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHS 120

Query: 121 SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTE 180
           SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTI+RGVSTE
Sbjct: 121 SHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIKRGVSTE 180

Query: 181 DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK 240
           DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK
Sbjct: 181 DTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGK 240

Query: 241 SATFLG-----WI-IRL-------IYAVSFVKDARVVHELKDYLKSTFLYSCDC------ 300
           SAT        W  I+         YAVSFVKDARVVHELKDYLKS     C+       
Sbjct: 241 SATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHELKDYLKS-----CNADIHVIV 300

Query: 301 KIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVT 360
           KIESADSIPNLH       G        AMVARGDLGAELPIEEVPLLQ           
Sbjct: 301 KIESADSIPNLHSILSASDG--------AMVARGDLGAELPIEEVPLLQ----------- 360

Query: 361 FLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVSFQEKLLM 414
                 IKRCRSMQK VIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAV     +L 
Sbjct: 361 ---EDIIKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAV-----MLS 419

BLAST of IVF0001299 vs. NCBI nr
Match: XP_038877819.1 (pyruvate kinase isozyme G, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 551 bits (1421), Expect = 1.27e-188
Identity = 324/458 (70.74%), Postives = 333/458 (72.71%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD----------------------- 60
           MAT N STGVPLLKSDSTRVADRLASCRIVSDAFGL+                       
Sbjct: 1   MATSNLSTGVPLLKSDSTRVADRLASCRIVSDAFGLEAKSGRMCLQSKQIASVRCLRIAE 60

Query: 61  ---------------DVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
                          DVSNSTFELQSNAF  SRTKLTTKSRRKTKIVCTIGPST+SREMI
Sbjct: 61  QREIASYNGSLDTDQDVSNSTFELQSNAFPLSRTKLTTKSRRKTKIVCTIGPSTNSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQF DKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFTDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLKEGQEFNFTI+RGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSL VQSKTDD V
Sbjct: 181 PILLKEGQEFNFTIKRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLTVQSKTDDLV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATFLG-----WI-IRL-------IYAVSFVKDARVVHE 300
           KCVVIDGGELKSRRHLNVRGKSAT        W  I+         YAVSFVKDARVVHE
Sbjct: 241 KCVVIDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSTFLYSCDC------KIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAE 360
           LKDYLKS     C+       KIESADSIPNLH       G        AMVARGDLGAE
Sbjct: 301 LKDYLKS-----CNADIHVIVKIESADSIPNLHSILSASDG--------AMVARGDLGAE 360

Query: 361 LPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVS 401
           LPIEEVPLLQ                 IKRCR+MQK VIVATNMLESMIDHPTPTRAEVS
Sbjct: 361 LPIEEVPLLQ--------------EDIIKRCRTMQKPVIVATNMLESMIDHPTPTRAEVS 420

BLAST of IVF0001299 vs. NCBI nr
Match: XP_022156410.1 (pyruvate kinase isozyme G, chloroplastic [Momordica charantia])

HSP 1 Score: 531 bits (1367), Expect = 1.81e-180
Identity = 312/457 (68.27%), Postives = 327/457 (71.55%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD----------------------- 60
           MATFN STGVP+L+SDS RV+DRLASCR V+DAF L+                       
Sbjct: 1   MATFNLSTGVPILRSDSARVSDRLASCRTVADAFALEPKNGRMCLQSKISPIRCRSIAEQ 60

Query: 61  --------------DVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIW 120
                         DVSNS  +LQSN F  SRTKLTT SRRKTKIVCTIGPSTSSREMIW
Sbjct: 61  REVETCDGSLDTDQDVSNSPIKLQSNGFPLSRTKLTTNSRRKTKIVCTIGPSTSSREMIW 120

Query: 121 KLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKP 180
           KLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQF DKVIAIMLDTKGPEVRSGDVP+P
Sbjct: 121 KLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFKDKVIAIMLDTKGPEVRSGDVPQP 180

Query: 181 ILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVK 240
           ILLKEGQEFNFTI+RGVSTEDTVSVNYDDFVNDVEVGD LLVDGGMMSLAV+SKT D VK
Sbjct: 181 ILLKEGQEFNFTIKRGVSTEDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTKDLVK 240

Query: 241 CVVIDGGELKSRRHLNVRGKSATFLG-----WI-IRL-------IYAVSFVKDARVVHEL 300
           CVVIDGGELKSRRHLNVRGKSAT        W  I+         YAVSFVKDARVVHEL
Sbjct: 241 CVVIDGGELKSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDARVVHEL 300

Query: 301 KDYLKSTFLYSCDC------KIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAEL 360
           KDYLKS     C+       KIESADSIPNLH       G        AMVARGDLGAEL
Sbjct: 301 KDYLKS-----CNADIYVIVKIESADSIPNLHSILSASDG--------AMVARGDLGAEL 360

Query: 361 PIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVSD 401
           PIEEVPLLQ +               IKRCRSMQK VIVATNMLESMIDHPTPTRAEVSD
Sbjct: 361 PIEEVPLLQEEI--------------IKRCRSMQKPVIVATNMLESMIDHPTPTRAEVSD 420

BLAST of IVF0001299 vs. NCBI nr
Match: KAG6571832.1 (hypothetical protein SDJN03_28560, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 526 bits (1355), Expect = 1.22e-178
Identity = 306/449 (68.15%), Postives = 327/449 (72.83%), Query Frame = 0

Query: 1   MATFNFSTGVPLLKSDSTRVADRLASCRIVSDAFGLD----------------------- 60
           MATF  STGVPLLKSDSTRVADRLASCR VSDAFG++                       
Sbjct: 1   MATFYLSTGVPLLKSDSTRVADRLASCRNVSDAFGIEAKSGRMYVQSKQIALIRCSRIAE 60

Query: 61  ---------------DVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMI 120
                          D+SNSTF+ QSN F  SR KLTTKSRRKTKIVCTIGPSTSSREMI
Sbjct: 61  KQDVASYKGSLDTDQDISNSTFDHQSNGFPLSRMKLTTKSRRKTKIVCTIGPSTSSREMI 120

Query: 121 WKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPK 180
           WKLAE GMNVARLNMSHGDHS+HQKTIDLVKEYNAQF DKVIAIMLDTKGPEVRSGDVPK
Sbjct: 121 WKLAEMGMNVARLNMSHGDHSTHQKTIDLVKEYNAQFKDKVIAIMLDTKGPEVRSGDVPK 180

Query: 181 PILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSV 240
           PILLK+GQEFNFTI+RGVSTEDTVSVNYDDF+NDVEVGD++LVDGGMMSL V+SKT+D V
Sbjct: 181 PILLKDGQEFNFTIKRGVSTEDTVSVNYDDFINDVEVGDSVLVDGGMMSLTVKSKTNDLV 240

Query: 241 KCVVIDGGELKSRRHLNVRGKSATFLG-----WI-IRL-------IYAVSFVKDARVVHE 300
           KCVV+DGGELKSRRHLNVRGKSAT        W  I+         YAVSFVKDARVVHE
Sbjct: 241 KCVVVDGGELKSRRHLNVRGKSATLPSITDKDWDDIKFGVDNQVDFYAVSFVKDARVVHE 300

Query: 301 LKDYLKSTFLYSCDC------KIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAE 360
           LKDYLKS     C+       KIESADSIPNLH       G        AMVARGDLGAE
Sbjct: 301 LKDYLKS-----CNADIHVIVKIESADSIPNLHSILSASDG--------AMVARGDLGAE 360

Query: 361 LPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVS 392
           LPIEEVPLLQ +T+             IKRC+SMQK VIVATNMLESMIDHPTPTRAEVS
Sbjct: 361 LPIEEVPLLQ-ETI-------------IKRCQSMQKPVIVATNMLESMIDHPTPTRAEVS 417

BLAST of IVF0001299 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 421.8 bits (1083), Expect = 8.8e-118
Identity = 263/457 (57.55%), Postives = 306/457 (66.96%), Query Frame = 0

Query: 33  AFGLDDVSNSTFELQSNAFHFSRTKLTTKSRRKTKIVCTIGPSTSSREMIWKLAETGMNV 92
           AF +  + +S++ L       SRT  +  SRRKTKIVCTIGPS+SSREMIWKLAE GMNV
Sbjct: 74  AFDVGVLDSSSYRLAD-----SRTS-SNDSRRKTKIVCTIGPSSSSREMIWKLAEAGMNV 133

Query: 93  ARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIMLDTKGPEVRSGDVPKPILLKEGQEF 152
           ARLNMSHGDH+SHQ TIDLVKEYN+ F DK IAIMLDTKGPEVRSGDVP+PI L+EGQEF
Sbjct: 134 ARLNMSHGDHASHQITIDLVKEYNSLFVDKAIAIMLDTKGPEVRSGDVPQPIFLEEGQEF 193

Query: 153 NFTIRRGVSTEDTVSVNYDDFVNDVEVGDTLLVDGGMMSLAVQSKTDDSVKCVVIDGGEL 212
           NFTI+RGVS +DTVSVNYDDFVNDVEVGD LLVDGGMMSLAV+SKT D VKCVVIDGGEL
Sbjct: 194 NFTIKRGVSLKDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGEL 253

Query: 213 KSRRHLNVRGKSATF-----LGW-IIRL-------IYAVSFVKDARVVHELKDYLKSTFL 272
           +SRRHLNVRGKSAT        W  I+         YAVSFVKDA+VVHELK+YLK+   
Sbjct: 254 QSRRHLNVRGKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCSA 313

Query: 273 -YSCDCKIESADSIPNLHRYFQHQMGLAFYTIRIAMVARGDLGAELPIEEVPLLQVKTLT 332
             S   KIESADSI NL        G        AMVARGDLGAELPIEEVPLLQ +   
Sbjct: 314 DISVIVKIESADSIKNLPSIISACDG--------AMVARGDLGAELPIEEVPLLQEE--- 373

Query: 333 HHITVTFLSGGYIKRCRSMQKTVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVSF 392
                       I+RCRS+ K VIVATNMLESMI+HPTPTRAEVSDIAIAVREGADA+  
Sbjct: 374 -----------IIRRCRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAI-- 433

Query: 393 QEKLLMGSTFLAHYLGRRQKNIH-LFKRNGSTVHMNTKINVWNYIVDHLLYSIFFKTSFL 452
              +L G T    +  +    +H +  R  +++ + T  +       H+     F  S +
Sbjct: 434 ---MLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTTAYKGHMGQMFAFHASIM 493

Query: 453 -QIICGYFLYLSRKGRVGMYKSHLYGRPTIIV-TDQK 473
              +    +  +R G + +  SH     TI   T+Q+
Sbjct: 494 ANTLSSPLIVFTRTGSMAVLLSHYRPSATIFAFTNQR 497

BLAST of IVF0001299 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 404.8 bits (1039), Expect = 1.1e-112
Identity = 226/328 (68.90%), Postives = 245/328 (74.70%), Query Frame = 0

Query: 63  RRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDK 122
           RRKTKIVCT+GPST++REMIWKLAE GMNVAR+NMSHGDH+SH+K IDLVKEYNAQ  D 
Sbjct: 109 RRKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDN 168

Query: 123 VIAIMLDTKGPEVRSGDVPKPILLKEGQEFNFTIRRGVSTEDTVSVNYDDFVNDVEVGDT 182
            IAIMLDTKGPEVRSGD+P+PI+L  GQEF FTI RGVST   VSVNYDDFVNDVE GD 
Sbjct: 169 TIAIMLDTKGPEVRSGDLPQPIMLDPGQEFTFTIERGVSTPSCVSVNYDDFVNDVEAGDM 228

Query: 183 LLVDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLNVRGKSATF------------LGW 242
           LLVDGGMMS  V+SKT DSVKC V+DGGELKSRRHLNVRGKSAT              G 
Sbjct: 229 LLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLNVRGKSATLPSITEKDWEDIKFGV 288

Query: 243 IIRL-IYAVSFVKDARVVHELKDYLKSTFL-YSCDCKIESADSIPNLHRYFQHQMGLAFY 302
             ++  YAVSFVKDA+VVHELK YL+++        KIESADSIPNLH       G    
Sbjct: 289 ENKVDFYAVSFVKDAQVVHELKKYLQNSGADIHVIVKIESADSIPNLHSIITASDG---- 348

Query: 303 TIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQKTVIVATNML 362
               AMVARGDLGAELPIEEVP+LQ +               I  CRSM K VIVATNML
Sbjct: 349 ----AMVARGDLGAELPIEEVPILQEE--------------IINLCRSMGKAVIVATNML 408

Query: 363 ESMIDHPTPTRAEVSDIAIAVREGADAV 377
           ESMI HPTPTRAEVSDIAIAVREGADAV
Sbjct: 409 ESMIVHPTPTRAEVSDIAIAVREGADAV 414

BLAST of IVF0001299 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 213.0 bits (541), Expect = 6.2e-55
Identity = 152/405 (37.53%), Postives = 214/405 (52.84%), Query Frame = 0

Query: 9   GVPLLKSDSTRVADRLASCRIVSDAFGLDDVSNSTFELQSNAFHFSRTKLTTKSRRKTKI 68
           G   +KSD   V     +  + +D  G++  + +  EL+ N F         +S R+TK+
Sbjct: 75  GTGAVKSDERSV----VATAVTTDTSGIEVDTVTEAELKENGF---------RSTRRTKL 134

Query: 69  VCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVIAIML 128
           +CTIGP+T   E +  LA  GMNVARLNM HG    H+  I  V+  N +     +AIM+
Sbjct: 135 ICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEE-KGFAVAIMM 194

Query: 129 DTKGPEVRSGDVPKPILLK--EGQEFNFTIRRGVST--EDTVSVNYDDFVNDVEVGDTLL 188
           DT+G E+  GD+      K  +G+ + FT+R   S+  E T+SV+YD F  DV VGD LL
Sbjct: 195 DTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVGDELL 254

Query: 189 VDGGMMSLAVQSKTDDSVKCVVIDGGELKSRRHLN-------VRGKSA-----TFLGWI- 248
           VDGGM+   V  K    VKC+  D G L  R +L        VR ++A     +   W+ 
Sbjct: 255 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDWLD 314

Query: 249 --------IRLIYAVSFVKDARVVHELKDYLKSTFL---YSCDCKIESADSIPNLHRYFQ 308
                   +  I AVSFVK A V++ LK YL +           KIES DS+ NL     
Sbjct: 315 IDFGIAEGVDFI-AVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEEIIL 374

Query: 309 HQMGLAFYTIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQKT 368
              G        AMVARGDLGA++P+E+VP  Q +               ++ CR++ K 
Sbjct: 375 ASDG--------AMVARGDLGAQIPLEQVPAAQQR--------------IVQVCRALNKP 434

Query: 369 VIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVSFQEKLLMG 386
           VIVA+ +LESMI++PTPTRAEV+D++ AVR+ +DA+    +  MG
Sbjct: 435 VIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMG 442

BLAST of IVF0001299 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 173.7 bits (439), Expect = 4.2e-43
Identity = 128/346 (36.99%), Postives = 179/346 (51.73%), Query Frame = 0

Query: 65  KTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDKVI 124
           KTKIVCT+GP++ +  MI KL + GMNVAR N SHG H  HQ+T+D ++  +A  N  ++
Sbjct: 21  KTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLR--SAMHNTGIL 80

Query: 125 -AIMLDTKGPEVRSGDVP--KPILLKEGQEFNFTIRRGV-STEDTVSVNYDDFVNDVEVG 184
            A+MLDTKGPE+R+G +    PI LKEGQE   T    +   E T+S++Y     DV+ G
Sbjct: 81  AAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITTDYDIQGDESTISMSYKKLPLDVKPG 140

Query: 185 DTLLVDGGMMSLAVQSKTDDS--VKCVVIDGGELKSRRHLNVRG-----------KSATF 244
           +T+L   G +SLAV S   +S  V+C   +   L  R+++N+ G                
Sbjct: 141 NTILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKNVNLPGVVVDLPTLTDKDIEDI 200

Query: 245 LGWIIRL---IYAVSFVKD----ARVVHELKDYLKSTFLYSCDCKIESADSIPNLHRYFQ 304
           LGW +     + A+SFV+       V   L  + KS  L S   K+E+ + + N     +
Sbjct: 201 LGWGVPNSIDMIALSFVRKGSDLVNVRKVLGSHAKSIMLMS---KVENQEGVINFDEILR 260

Query: 305 HQMGLAFYTIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQKT 364
                        MVARGDLG E+PIE++ L Q                 I +C    K 
Sbjct: 261 ETDAF--------MVARGDLGMEIPIEKIFLAQKL--------------MIYKCNLAGKP 320

Query: 365 VIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVSFQEKLLMGS 387
           V+ AT MLESMI  P PTRAE +D+A AV +G D V    +   G+
Sbjct: 321 VVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGA 339

BLAST of IVF0001299 vs. TAIR 10
Match: AT3G55650.1 (Pyruvate kinase family protein )

HSP 1 Score: 173.3 bits (438), Expect = 5.4e-43
Identity = 125/348 (35.92%), Postives = 186/348 (53.45%), Query Frame = 0

Query: 63  RRKTKIVCTIGPSTSSREMIWKLAETGMNVARLNMSHGDHSSHQKTIDLVKEYNAQFNDK 122
           R KTKI+CT+GP + S EMI KL + GMNVAR N SHG HS HQ+T+D ++   A  N  
Sbjct: 15  RSKTKIICTLGPVSRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLR--TAMDNTG 74

Query: 123 VI-AIMLDTKGPEVRSGDVP--KPILLKEGQEFNFTIRRGVSTE-DTVSVNYDDFVNDVE 182
           ++ A+MLDTKGPE+R+G +   KPI L +GQE   +I   +  + + +S++Y     DV+
Sbjct: 75  ILSAVMLDTKGPEIRTGFLKEGKPIQLNQGQEITISIDYMIEGDSNVISMSYKKLAEDVK 134

Query: 183 VGDTLLVDGGMMSLAVQS--KTDDSVKCVVIDGGELKSRRHLNVRG-----------KSA 242
            GD +L   G +SL V S  K+   V+C   +   L  R+++N+ G              
Sbjct: 135 PGDVILCSDGTISLTVLSCDKSFGLVRCRCENSAILGERKNVNLPGIVVDLPTLTEKDKE 194

Query: 243 TFLGWIIRL---IYAVSFVKDARVVHELK----DYLKSTFLYSCDCKIESADSIPNLHRY 302
             + W +     I A+SFV+    + E++    ++ K+  L S   K+E+ + + N  + 
Sbjct: 195 DIIQWGVPNKIDIIALSFVRKGSDLTEVRRLLGEHSKNIMLMS---KVENQEGVMNCEKI 254

Query: 303 FQHQMGLAFYTIRIAMVARGDLGAELPIEEVPLLQVKTLTHHITVTFLSGGYIKRCRSMQ 362
            ++            MVARGDLG E+PIE++ L Q KT+             IK   ++ 
Sbjct: 255 LENSDAF--------MVARGDLGMEIPIEKMFLAQ-KTM-------------IKMANALG 314

Query: 363 KTVIVATNMLESMIDHPTPTRAEVSDIAIAVREGADAVSFQEKLLMGS 387
           K V+ AT MLESM   P PTRAE +D+A AV +G D V    +   G+
Sbjct: 315 KPVVTATQMLESMTVSPRPTRAEATDVANAVLDGTDCVMLSGETAAGA 335