IVF0000987 (gene) Melon (IVF77) v1

Overview
NameIVF0000987
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionSplicing factor YJU2
Locationchr10: 24582734 .. 24583142 (+)
RNA-Seq ExpressionIVF0000987
SyntenyIVF0000987
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGAGAGGAAGGTGCTTAACAAATACTACCCACCGGATTTTGATCCGTCAAAGCTACCAAGGGTTCGTAGACCCAAAAACCAGCAAATGAAGGTTCGTATGATGCTTCCTATGAGCATCCGGTGCAATACTTGTGGTAATTACATATACAAGGGCACAAAGTTCAATTCTCGCAAGGAGGACGTCATTGGTGAGGCAAGGGAATCCCTTTTATATATTTTTTATATTTTTAACCTAAAAGATGTTTATTCCTTTTGTAATTGATTTTCTGATTTCTCATGTATTTTTTTCCTTTTATTTCAGACATATTTGGGAATTCAAATTTTTAGGTTCTACTTCAAGTGTACCAGATGTTCTGCTGAGCTTACCATTAAAACAGACCCCCAAAACTCAGACTACGTTGTAG

mRNA sequence

ATGGGTGAGAGGAAGGTGCTTAACAAATACTACCCACCGGATTTTGATCCGTCAAAGCTACCAAGGGTTCGTAGACCCAAAAACCAGCAAATGAAGGTTCGTATGATGCTTCCTATGAGCATCCGGTGCAATACTTGTGGTAATTACATATACAAGGGCACAAAGTTCAATTCTCGCAAGGAGGACGTCATTGGTTCTACTTCAAGTGTACCAGATGTTCTGCTGAGCTTACCATTAAAACAGACCCCCAAAACTCAGACTACGTTGTAG

Coding sequence (CDS)

ATGGGTGAGAGGAAGGTGCTTAACAAATACTACCCACCGGATTTTGATCCGTCAAAGCTACCAAGGGTTCGTAGACCCAAAAACCAGCAAATGAAGGTTCGTATGATGCTTCCTATGAGCATCCGGTGCAATACTTGTGGTAATTACATATACAAGGGCACAAAGTTCAATTCTCGCAAGGAGGACGTCATTGGTTCTACTTCAAGTGTACCAGATGTTCTGCTGAGCTTACCATTAAAACAGACCCCCAAAACTCAGACTACGTTGTAG

Protein sequence

MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRKEDVIGSTSSVPDVLLSLPLKQTPKTQTTL
Homology
BLAST of IVF0000987 vs. ExPASy Swiss-Prot
Match: Q9BW85 (Splicing factor YJU2 OS=Homo sapiens OX=9606 GN=YJU2 PE=1 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 5.8e-21
Identity = 47/79 (59.49%), Postives = 59/79 (74.68%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          M ERKVLNKYYPPDFDPSK+P+++ PK++Q  VR+M P ++RC TCG YIYKG KFN+RK
Sbjct: 1  MSERKVLNKYYPPDFDPSKIPKLKLPKDRQYVVRLMAPFNMRCKTCGEYIYKGKKFNARK 60

Query: 61 EDVIGSTSSVPDVLLSLPL 80
          E V        +V L LP+
Sbjct: 61 ETVQN------EVYLGLPI 73

BLAST of IVF0000987 vs. ExPASy Swiss-Prot
Match: A8WHR3 (Splicing factor YJU2 OS=Danio rerio OX=7955 GN=yju2 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 7.6e-21
Identity = 43/63 (68.25%), Postives = 53/63 (84.13%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          M ERKVLNKYYPPDFDPSK+P+++ PK++Q  VR+M P ++RC TCG YIYKG KFN+RK
Sbjct: 1  MSERKVLNKYYPPDFDPSKIPKLKLPKDRQYVVRLMAPFNMRCKTCGEYIYKGKKFNARK 60

Query: 61 EDV 64
          E V
Sbjct: 61 ETV 63

BLAST of IVF0000987 vs. ExPASy Swiss-Prot
Match: Q9D6J3 (Splicing factor YJU2 OS=Mus musculus OX=10090 GN=Yju2 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 7.6e-21
Identity = 43/63 (68.25%), Postives = 53/63 (84.13%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          M ERKVLNKYYPPDFDPSK+P+++ PK++Q  VR+M P ++RC TCG YIYKG KFN+RK
Sbjct: 1  MSERKVLNKYYPPDFDPSKIPKLKLPKDRQYVVRLMAPFNMRCKTCGEYIYKGKKFNARK 60

Query: 61 EDV 64
          E V
Sbjct: 61 ETV 63

BLAST of IVF0000987 vs. ExPASy Swiss-Prot
Match: Q54WR5 (Splicing factor YJU2 OS=Dictyostelium discoideum OX=44689 GN=yju2 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 3.5e-18
Identity = 41/63 (65.08%), Postives = 51/63 (80.95%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKV++KYYPPDFDPSK+ +++  +    KV  MLPMSIRCNTCG YI +GTKFN++K
Sbjct: 1  MGERKVISKYYPPDFDPSKVAKIKVKRPTFTKVTTMLPMSIRCNTCGEYIGRGTKFNAKK 60

Query: 61 EDV 64
          E V
Sbjct: 61 ETV 63

BLAST of IVF0000987 vs. ExPASy Swiss-Prot
Match: Q9P7C5 (Splicing factor YJU2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=cwf16 PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 4.8e-15
Identity = 39/66 (59.09%), Postives = 48/66 (72.73%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQ-----QMKVRMMLPMSIRCNTCGNYIYKGTK 60
          M ERKVLNKY PPD+DPS  P  ++ K Q     ++ VR+M P S+RC+TCG YIYKG K
Sbjct: 1  MSERKVLNKYIPPDYDPSIRPPKKKKKFQGPNGGKLTVRLMTPFSMRCHTCGEYIYKGKK 60

Query: 61 FNSRKE 62
          FN+RKE
Sbjct: 61 FNARKE 66

BLAST of IVF0000987 vs. ExPASy TrEMBL
Match: A0A0B0MV50 (Splicing factor YJU2 OS=Gossypium arboreum OX=29729 GN=LOC108474488 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.4e-31
Identity = 66/67 (98.51%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGST 68
          EDVIG T
Sbjct: 61 EDVIGET 67

BLAST of IVF0000987 vs. ExPASy TrEMBL
Match: A0A6P9EB24 (Splicing factor YJU2 OS=Juglans regia OX=51240 GN=LOC109011793 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.4e-31
Identity = 66/67 (98.51%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGST 68
          EDVIG T
Sbjct: 61 EDVIGET 67

BLAST of IVF0000987 vs. ExPASy TrEMBL
Match: A0A6P9ED46 (Splicing factor YJU2 OS=Juglans regia OX=51240 GN=LOC118348244 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.4e-31
Identity = 66/67 (98.51%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGST 68
          EDVIG T
Sbjct: 61 EDVIGET 67

BLAST of IVF0000987 vs. ExPASy TrEMBL
Match: A0A6J1AVA5 (Splicing factor YJU2 OS=Herrania umbratica OX=108875 GN=LOC110421628 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.4e-31
Identity = 66/67 (98.51%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGST 68
          EDVIG T
Sbjct: 61 EDVIGET 67

BLAST of IVF0000987 vs. ExPASy TrEMBL
Match: A0A5D2RD26 (Splicing factor YJU2 OS=Gossypium tomentosum OX=34277 GN=ES332_A02G008700v1 PE=3 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 8.4e-31
Identity = 66/67 (98.51%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGST 68
          EDVIG T
Sbjct: 61 EDVIGET 67

BLAST of IVF0000987 vs. NCBI nr
Match: XP_008466970.1 (PREDICTED: coiled-coil domain-containing protein 94 homolog, partial [Cucumis melo])

HSP 1 Score: 140 bits (354), Expect = 4.59e-41
Identity = 66/67 (98.51%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGST 67
          EDVIG T
Sbjct: 61 EDVIGET 67

BLAST of IVF0000987 vs. NCBI nr
Match: KAF9840338.1 (hypothetical protein H0E87_008065 [Populus deltoides])

HSP 1 Score: 139 bits (349), Expect = 7.02e-40
Identity = 64/67 (95.52%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDPSKLPR+RRPKNQQ+KVRMMLPMSIRCNTCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPSKLPRIRRPKNQQIKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGST 67
          EDVIG T
Sbjct: 61 EDVIGET 67

BLAST of IVF0000987 vs. NCBI nr
Match: XP_016903347.1 (PREDICTED: coiled-coil domain-containing protein 94 homolog, partial [Cucumis melo])

HSP 1 Score: 137 bits (345), Expect = 9.27e-40
Identity = 65/67 (97.01%), Postives = 65/67 (97.01%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGN IYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNCIYKGTKFNSRK 60

Query: 61 EDVIGST 67
          EDVIG T
Sbjct: 61 EDVIGET 67

BLAST of IVF0000987 vs. NCBI nr
Match: KAF9839281.1 (hypothetical protein H0E87_007259 [Populus deltoides])

HSP 1 Score: 143 bits (360), Expect = 1.13e-39
Identity = 67/77 (87.01%), Postives = 72/77 (93.51%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDPSKLPR+RRPKNQQ+KVRMMLPMSIRCNTCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPSKLPRIRRPKNQQIKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGSTSSVPDVLLSL 77
          EDVIG +  VP  +L+L
Sbjct: 61 EDVIGESKVVPRGILNL 77

BLAST of IVF0000987 vs. NCBI nr
Match: CBI14833.3 (unnamed protein product, partial [Vitis vinifera])

HSP 1 Score: 139 bits (349), Expect = 1.98e-39
Identity = 64/67 (95.52%), Postives = 66/67 (98.51%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDPSKLPR+RRPKNQQ+KVRMMLPMSIRCNTCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPSKLPRIRRPKNQQIKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGST 67
          EDVIG T
Sbjct: 61 EDVIGET 67

BLAST of IVF0000987 vs. TAIR 10
Match: AT1G17130.1 (Family of unknown function (DUF572) )

HSP 1 Score: 132.9 bits (333), Expect = 1.3e-31
Identity = 60/67 (89.55%), Postives = 64/67 (95.52%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDP+KL R+RRPKNQQ+KVRMMLPMS+RC TCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPAKLQRLRRPKNQQIKVRMMLPMSVRCGTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGST 68
          EDVIG T
Sbjct: 61 EDVIGET 67

BLAST of IVF0000987 vs. TAIR 10
Match: AT1G17130.2 (Family of unknown function (DUF572) )

HSP 1 Score: 131.7 bits (330), Expect = 2.9e-31
Identity = 60/69 (86.96%), Postives = 64/69 (92.75%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERKVLNKYYPPDFDP+KL R+RRPKNQQ+KVRMMLPMS+RC TCGNYIYKGTKFNSRK
Sbjct: 1  MGERKVLNKYYPPDFDPAKLQRLRRPKNQQIKVRMMLPMSVRCGTCGNYIYKGTKFNSRK 60

Query: 61 EDVIGSTSS 70
          EDVIG   S
Sbjct: 61 EDVIGENFS 69

BLAST of IVF0000987 vs. TAIR 10
Match: AT3G43250.1 (Family of unknown function (DUF572) )

HSP 1 Score: 110.2 bits (274), Expect = 8.9e-25
Identity = 47/67 (70.15%), Postives = 57/67 (85.07%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERK LNKYYPPDFDP K+ R+++PKNQQ K+R MLP+ +RCNTCGNY+ +GTKFN R+
Sbjct: 1  MGERKNLNKYYPPDFDPKKIHRIKKPKNQQKKIRFMLPVRVRCNTCGNYMSEGTKFNCRQ 60

Query: 61 EDVIGST 68
          EDVI  T
Sbjct: 61 EDVITET 67

BLAST of IVF0000987 vs. TAIR 10
Match: AT2G32050.1 (Family of unknown function (DUF572) )

HSP 1 Score: 109.4 bits (272), Expect = 1.5e-24
Identity = 46/67 (68.66%), Postives = 58/67 (86.57%), Query Frame = 0

Query: 1  MGERKVLNKYYPPDFDPSKLPRVRRPKNQQMKVRMMLPMSIRCNTCGNYIYKGTKFNSRK 60
          MGERK LNKYYPP+FDP ++PR+R+PKNQQ K+R M+P+ IRCNTCGNY+ +GTK N R+
Sbjct: 1  MGERKGLNKYYPPNFDPKQIPRIRKPKNQQRKIRSMVPLRIRCNTCGNYMSEGTKINCRE 60

Query: 61 EDVIGST 68
          E+VIG T
Sbjct: 61 ENVIGET 67

BLAST of IVF0000987 vs. TAIR 10
Match: AT2G29430.1 (Family of unknown function (DUF572) )

HSP 1 Score: 47.4 bits (111), Expect = 7.1e-06
Identity = 20/33 (60.61%), Postives = 24/33 (72.73%), Query Frame = 0

Query: 35 MMLPMSIRCNTCGNYIYKGTKFNSRKEDVIGST 68
          + LPM ++CN C N + KGTKF SR EDVIG T
Sbjct: 8  LSLPMRLQCNNCDNIMSKGTKFTSRVEDVIGET 40

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9BW855.8e-2159.49Splicing factor YJU2 OS=Homo sapiens OX=9606 GN=YJU2 PE=1 SV=1[more]
A8WHR37.6e-2168.25Splicing factor YJU2 OS=Danio rerio OX=7955 GN=yju2 PE=1 SV=1[more]
Q9D6J37.6e-2168.25Splicing factor YJU2 OS=Mus musculus OX=10090 GN=Yju2 PE=1 SV=1[more]
Q54WR53.5e-1865.08Splicing factor YJU2 OS=Dictyostelium discoideum OX=44689 GN=yju2 PE=3 SV=1[more]
Q9P7C54.8e-1559.09Splicing factor YJU2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=2... [more]
Match NameE-valueIdentityDescription
A0A0B0MV508.4e-3198.51Splicing factor YJU2 OS=Gossypium arboreum OX=29729 GN=LOC108474488 PE=3 SV=1[more]
A0A6P9EB248.4e-3198.51Splicing factor YJU2 OS=Juglans regia OX=51240 GN=LOC109011793 PE=3 SV=1[more]
A0A6P9ED468.4e-3198.51Splicing factor YJU2 OS=Juglans regia OX=51240 GN=LOC118348244 PE=3 SV=1[more]
A0A6J1AVA58.4e-3198.51Splicing factor YJU2 OS=Herrania umbratica OX=108875 GN=LOC110421628 PE=3 SV=1[more]
A0A5D2RD268.4e-3198.51Splicing factor YJU2 OS=Gossypium tomentosum OX=34277 GN=ES332_A02G008700v1 PE=3... [more]
Match NameE-valueIdentityDescription
XP_008466970.14.59e-4198.51PREDICTED: coiled-coil domain-containing protein 94 homolog, partial [Cucumis me... [more]
KAF9840338.17.02e-4095.52hypothetical protein H0E87_008065 [Populus deltoides][more]
XP_016903347.19.27e-4097.01PREDICTED: coiled-coil domain-containing protein 94 homolog, partial [Cucumis me... [more]
KAF9839281.11.13e-3987.01hypothetical protein H0E87_007259 [Populus deltoides][more]
CBI14833.31.98e-3995.52unnamed protein product, partial [Vitis vinifera][more]
Match NameE-valueIdentityDescription
AT1G17130.11.3e-3189.55Family of unknown function (DUF572) [more]
AT1G17130.22.9e-3186.96Family of unknown function (DUF572) [more]
AT3G43250.18.9e-2570.15Family of unknown function (DUF572) [more]
AT2G32050.11.5e-2468.66Family of unknown function (DUF572) [more]
AT2G29430.17.1e-0660.61Family of unknown function (DUF572) [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007590Saf4/Yju2 proteinPFAMPF04502DUF572coord: 9..65
e-value: 4.5E-31
score: 108.7
IPR007590Saf4/Yju2 proteinPANTHERPTHR12111SPLICING FACTOR YJU2coord: 1..67
NoneNo IPR availablePANTHERPTHR12111:SF7BNAA02G14200D PROTEINcoord: 1..67

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0000987.1IVF0000987.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000349 generation of catalytic spliceosome for first transesterification step
cellular_component GO:0071006 U2-type catalytic step 1 spliceosome
molecular_function GO:0046872 metal ion binding