HG10021721 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021721 vs. NCBI nr
Match: XP_031739140.1 (secoisolariciresinol dehydrogenase [Cucumis sativus] >XP_031745375.1 secoisolariciresinol dehydrogenase-like [Cucumis sativus])

HSP 1 Score: 463.8 bits (1192), Expect = 1.1e-126
Identity = 228/266 (85.71%), Postives = 247/266 (92.86%), Query Frame = 0

Query: 17  DDLRLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVH 76
           +D RL+GK AIITG ARGIGES A+HFF  GAKVVIADILDDLG SLSNHLSSSS+SFVH
Sbjct: 11  EDNRLEGKAAIITGGARGIGESIAKHFFNPGAKVVIADILDDLGNSLSNHLSSSSTSFVH 70

Query: 77  CDVTKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAF 136
           C+VTKETDVEN +NTA+SKYGKLDIMFNNAGI G LKFNILENE SDF+NVLNVNL+GAF
Sbjct: 71  CNVTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLVGAF 130

Query: 137 LGTKHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIR 196
           LGTKHAAKAMIP GQGSI+++ASVCS IGGIGPYAYTSSKHGLLGLMRNA +DLGRYGI+
Sbjct: 131 LGTKHAAKAMIPAGQGSIVITASVCSSIGGIGPYAYTSSKHGLLGLMRNATIDLGRYGIK 190

Query: 197 VNCVSPHVVPTQMTRELFKVEDGDEFPNVYSH-FKCGDILRDKDVAEVALYLASDASRFV 256
           VNCVSPHVVPTQMTRE FK++DGDEFP+VYSH FKCGDILR +DVAEV LYLASDASRFV
Sbjct: 191 VNCVSPHVVPTQMTREHFKLKDGDEFPDVYSHNFKCGDILRKEDVAEVGLYLASDASRFV 250

Query: 257 SGHNFILDGGFTAGNQGLCSYQQSGN 282
           SGHNF+LDGGFTAGNQGLCSYQQ GN
Sbjct: 251 SGHNFVLDGGFTAGNQGLCSYQQFGN 276

BLAST of HG10021721 vs. NCBI nr
Match: KAE8637559.1 (hypothetical protein CSA_004626 [Cucumis sativus])

HSP 1 Score: 462.2 bits (1188), Expect = 3.1e-126
Identity = 227/263 (86.31%), Postives = 245/263 (93.16%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDV 79
           RL+GK AIITG ARGIGES A+HFF  GAKVVIADILDDLG SLSNHLSSSS+SFVHC+V
Sbjct: 48  RLEGKAAIITGGARGIGESIAKHFFNPGAKVVIADILDDLGNSLSNHLSSSSTSFVHCNV 107

Query: 80  TKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGT 139
           TKETDVEN +NTA+SKYGKLDIMFNNAGI G LKFNILENE SDF+NVLNVNL+GAFLGT
Sbjct: 108 TKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLVGAFLGT 167

Query: 140 KHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVNC 199
           KHAAKAMIP GQGSI+++ASVCS IGGIGPYAYTSSKHGLLGLMRNA +DLGRYGI+VNC
Sbjct: 168 KHAAKAMIPAGQGSIVITASVCSSIGGIGPYAYTSSKHGLLGLMRNATIDLGRYGIKVNC 227

Query: 200 VSPHVVPTQMTRELFKVEDGDEFPNVYSH-FKCGDILRDKDVAEVALYLASDASRFVSGH 259
           VSPHVVPTQMTRE FK++DGDEFP+VYSH FKCGDILR +DVAEV LYLASDASRFVSGH
Sbjct: 228 VSPHVVPTQMTREHFKLKDGDEFPDVYSHNFKCGDILRKEDVAEVGLYLASDASRFVSGH 287

Query: 260 NFILDGGFTAGNQGLCSYQQSGN 282
           NF+LDGGFTAGNQGLCSYQQ GN
Sbjct: 288 NFVLDGGFTAGNQGLCSYQQFGN 310

BLAST of HG10021721 vs. NCBI nr
Match: KAE8652818.1 (hypothetical protein Csa_022860 [Cucumis sativus])

HSP 1 Score: 457.2 bits (1175), Expect = 1.0e-124
Identity = 225/263 (85.55%), Postives = 244/263 (92.78%), Query Frame = 0

Query: 19  LRLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCD 78
           L+ +GK AIITG ARGIGES A+HFF  GAKVVIADILDDLG SLSNHLSSSS+SFVHC+
Sbjct: 9   LQEEGKAAIITGGARGIGESIAKHFFNPGAKVVIADILDDLGNSLSNHLSSSSTSFVHCN 68

Query: 79  VTKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLG 138
           VTKETDVEN +NTA+SKYGKLDIMFNNAGI G LKFNILENE SDF+NVLNVNL+GAFLG
Sbjct: 69  VTKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLVGAFLG 128

Query: 139 TKHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVN 198
           TKHAAKAMIP GQGSI+++ASVCS IGGIGPYAYTSSKHGLLGLMRNA +DLGRYGI+VN
Sbjct: 129 TKHAAKAMIPAGQGSIVITASVCSSIGGIGPYAYTSSKHGLLGLMRNATIDLGRYGIKVN 188

Query: 199 CVSPHVVPTQMTRELFKVEDGDEFPNVYSH-FKCGDILRDKDVAEVALYLASDASRFVSG 258
           CVSPHVVPTQMTRE FK++DGDEFP+VYSH FKCGDILR +DVAEV LYLASDASRFVSG
Sbjct: 189 CVSPHVVPTQMTREHFKLKDGDEFPDVYSHNFKCGDILRKEDVAEVGLYLASDASRFVSG 248

Query: 259 HNFILDGGFTAGNQGLCSYQQSG 281
           HNF+LDGGFTAGNQGLCSYQQ G
Sbjct: 249 HNFVLDGGFTAGNQGLCSYQQFG 271

BLAST of HG10021721 vs. NCBI nr
Match: XP_022973162.1 (secoisolariciresinol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 456.8 bits (1174), Expect = 1.3e-124
Identity = 220/258 (85.27%), Postives = 242/258 (93.80%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDV 79
           RL+GKVA+ITGAARGIGESTA+ FFKHGAKVV+ADILDD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 80  TKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGT 139
           TKE D+ENA+NTAI+KYGKLDIM NNAGI+GTLKFN LENESSDF+NVLNVNLLG FLGT
Sbjct: 72  TKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVLNVNLLGTFLGT 131

Query: 140 KHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVNC 199
           KHAA+AMIP  QGSIIM+ASVCS+IGGIGPY YTSSKHG+LGLM+NA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 200 VSPHVVPTQMTRELFKVEDGDEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFVSGHN 259
           VSPHVVP+QMTR+LFK+EDGDEFPNVYSHFKCGDILR +D+AE ALYLASDASRFVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLASDASRFVSGHN 251

Query: 260 FILDGGFTAGNQGLCSYQ 278
            I+DGGFT+ NQGLCSYQ
Sbjct: 252 LIVDGGFTSANQGLCSYQ 269

BLAST of HG10021721 vs. NCBI nr
Match: XP_022927564.1 (secoisolariciresinol dehydrogenase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 454.9 bits (1169), Expect = 5.0e-124
Identity = 222/258 (86.05%), Postives = 241/258 (93.41%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDV 79
           RL+GKVA+ITGAARGIGESTA+ FFKHGAKVV+ADILDD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 80  TKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGT 139
           TKE D+ENA+NTAISKYGKLDIM NNAGI+GTLKFNILENE SDF+ VLNVNLLGAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 140 KHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVNC 199
           KHAAKAMIP  QGSII+ AS CSIIGGIGPYAYTSSKHGLLGLM+NA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 200 VSPHVVPTQMTRELFKVEDGDEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFVSGHN 259
           VSPHVVP+QMTR+LFK+EDGDEFPNVYSHFKCGDILR +D+AE ALYLASDASRFVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 260 FILDGGFTAGNQGLCSYQ 278
            I+DGGFT+ NQGLCSY+
Sbjct: 252 LIVDGGFTSANQGLCSYE 269

BLAST of HG10021721 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.1e-68
Identity = 129/260 (49.62%), Postives = 185/260 (71.15%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDV 79
           RL+GKVA+ITG A GIGE+TA+ F +HGAKV IAD+ D+LG S+   + +S+S+++HCDV
Sbjct: 14  RLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCDV 73

Query: 80  TKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGT 139
           T E  V+NA++  +S YGKLDIMF+NAGI+   +  I++NE +DF+ V +VN+ G FL  
Sbjct: 74  TNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFLCM 133

Query: 140 KHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVNC 199
           KHAA+ MIP   G+II +AS+ S +GG   +AY  SKH +LGL RN AV+LG++GIRVNC
Sbjct: 134 KHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRVNC 193

Query: 200 VSPHVVPTQMTRELFKVEDGDEFPNV--YSHFKCGDILRDKDVAEVALYLASDASRFVSG 259
           +SP  +PT + ++   +++ +EF NV  ++    G     +DVA  ALYLASD +++VSG
Sbjct: 194 LSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALYLASDEAKYVSG 253

Query: 260 HNFILDGGFTAGNQGLCSYQ 278
           HN  +DGGF+  N  +  +Q
Sbjct: 254 HNLFIDGGFSVCNSVIKVFQ 273

BLAST of HG10021721 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.6e-64
Identity = 127/255 (49.80%), Postives = 178/255 (69.80%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSS-SSFVHCD 79
           RL  KVAIITG A GIGE+TA+ F ++GAKVVIADI DD G+ + N++ S    SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 80  VTKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLG 139
           VTK+ DV N ++T I+K+GKLDIMF N G+  T  ++ILE  + DFK V+++N+ GAFL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 140 TKHAAKAMIPTGQGSIIMSASVCSIIGGIG-PYAYTSSKHGLLGLMRNAAVDLGRYGIRV 199
            KHAA+ MIP  +GSI+ +AS+ S   G G  + YT++KH +LGL  +   +LG YGIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIRV 192

Query: 200 NCVSPHVVPTQMTRELFKVEDG--DEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFV 259
           NCVSP++V + +  ++F V+    +E  +  ++ K G +LR +DVA+   YLA D S++V
Sbjct: 193 NCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLK-GTLLRAEDVADAVAYLAGDESKYV 252

Query: 260 SGHNFILDGGFTAGN 271
           SG N ++DGG+T  N
Sbjct: 253 SGLNLVIDGGYTRTN 266

BLAST of HG10021721 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 4.6e-64
Identity = 130/266 (48.87%), Postives = 184/266 (69.17%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILD----DLGRSLSNHLSSSSSSFV 79
           RL+GKVAIITG A GIG++T   F +HGA VVIAD+ +     L +SLS+H +S   +F+
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 80  HCDVTKETDVENAINTAISKYGKLDIMFNNAGITGTLK--FNILENESSDFKNVLNVNLL 139
            CDV+ E DVEN +N  +++YG+LDI+FNNAG+ G  K   +IL+ ++ +F +V+ VN+ 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 140 GAFLGTKHAAKAMIPTG-QGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGR 199
           G  LG KH A+AMI  G +G II +ASV  ++GG+GP+AYT+SKH ++GL +NAA +LG+
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 200 YGIRVNCVSPHVVPTQMTRELFK-----------VEDGDEFPNVYSHFKCGDILRDKDVA 259
           YGIRVNC+SP  V T M    ++           VE+ +EF    ++ K G+ LR  D+A
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLK-GETLRANDIA 270

Query: 260 EVALYLASDASRFVSGHNFILDGGFT 268
           E ALYLASD S++V+GHN ++DGG T
Sbjct: 271 EAALYLASDESKYVNGHNLVVDGGVT 295

BLAST of HG10021721 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 6.1e-64
Identity = 130/256 (50.78%), Postives = 171/256 (66.80%), Query Frame = 0

Query: 17  DDLRLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVH 76
           D  +L GKVA+ITG A GIG  TAR F KHGA+VV+ADI D+LG SL   L   +SS+VH
Sbjct: 11  DARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVH 70

Query: 77  CDVTKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAF 136
           CDVT E DV  A++ A++++GKLD+MFNNAG++G   F + E    DF+ VL VNL+G F
Sbjct: 71  CDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPF 130

Query: 137 LGTKHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIR 196
           LGTKHAA+ M P  +GSII +AS+ S + G   +AYT+SKH L+G   NAA +LGR+GIR
Sbjct: 131 LGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIR 190

Query: 197 VNCVSPHVVPTQMTRELFKVEDG--DEFPNVYSHFKCGDILRDKDVAEVALYLASDASRF 256
           VNCVSP  V T + R    ++D   +      ++ K    L+  D+A  AL+LASD  R+
Sbjct: 191 VNCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGRY 250

Query: 257 VSGHNFILDGGFTAGN 271
           VSG N  +DGG +  N
Sbjct: 251 VSGQNLRVDGGLSVVN 266

BLAST of HG10021721 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 7.9e-64
Identity = 127/255 (49.80%), Postives = 179/255 (70.20%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSS-SSFVHCD 79
           RL  KVAIITG A GIGE+TA+ F ++GAKVVIADI DD G+ + N++ S    SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFVHCD 72

Query: 80  VTKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLG 139
           VTK+ DV N ++T I+K+GKLDIMF N G+  T  ++ILE  + DFK V+++N+ GAFL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 140 TKHAAKAMIPTGQGSIIMSASVCSIIGGIG-PYAYTSSKHGLLGLMRNAAVDLGRYGIRV 199
            KHAA+ MIP  +GSI+ +AS+ S   G G  + YT++KH +LGL  +   +LG++GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHGIRV 192

Query: 200 NCVSPHVVPTQMTRELFKVEDG--DEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFV 259
           NCVSP+VV + +  ++F V+    +E  +  ++ K G +LR +DVA+   YLA D S++V
Sbjct: 193 NCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLK-GILLRAEDVADAVAYLAGDESKYV 252

Query: 260 SGHNFILDGGFTAGN 271
           SG N ++DGG+T  N
Sbjct: 253 SGLNLVIDGGYTRTN 266

BLAST of HG10021721 vs. ExPASy TrEMBL
Match: A0A0A0LT69 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G168890 PE=4 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.5e-126
Identity = 227/263 (86.31%), Postives = 245/263 (93.16%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDV 79
           RL+GK AIITG ARGIGES A+HFF  GAKVVIADILDDLG SLSNHLSSSS+SFVHC+V
Sbjct: 12  RLEGKAAIITGGARGIGESIAKHFFNPGAKVVIADILDDLGNSLSNHLSSSSTSFVHCNV 71

Query: 80  TKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGT 139
           TKETDVEN +NTA+SKYGKLDIMFNNAGI G LKFNILENE SDF+NVLNVNL+GAFLGT
Sbjct: 72  TKETDVENVVNTAVSKYGKLDIMFNNAGIPGALKFNILENEYSDFQNVLNVNLVGAFLGT 131

Query: 140 KHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVNC 199
           KHAAKAMIP GQGSI+++ASVCS IGGIGPYAYTSSKHGLLGLMRNA +DLGRYGI+VNC
Sbjct: 132 KHAAKAMIPAGQGSIVITASVCSSIGGIGPYAYTSSKHGLLGLMRNATIDLGRYGIKVNC 191

Query: 200 VSPHVVPTQMTRELFKVEDGDEFPNVYSH-FKCGDILRDKDVAEVALYLASDASRFVSGH 259
           VSPHVVPTQMTRE FK++DGDEFP+VYSH FKCGDILR +DVAEV LYLASDASRFVSGH
Sbjct: 192 VSPHVVPTQMTREHFKLKDGDEFPDVYSHNFKCGDILRKEDVAEVGLYLASDASRFVSGH 251

Query: 260 NFILDGGFTAGNQGLCSYQQSGN 282
           NF+LDGGFTAGNQGLCSYQQ GN
Sbjct: 252 NFVLDGGFTAGNQGLCSYQQFGN 274

BLAST of HG10021721 vs. ExPASy TrEMBL
Match: A0A6J1I6S5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471670 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 6.4e-125
Identity = 220/258 (85.27%), Postives = 242/258 (93.80%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDV 79
           RL+GKVA+ITGAARGIGESTA+ FFKHGAKVV+ADILDD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 80  TKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGT 139
           TKE D+ENA+NTAI+KYGKLDIM NNAGI+GTLKFN LENESSDF+NVLNVNLLG FLGT
Sbjct: 72  TKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVLNVNLLGTFLGT 131

Query: 140 KHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVNC 199
           KHAA+AMIP  QGSIIM+ASVCS+IGGIGPY YTSSKHG+LGLM+NA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 200 VSPHVVPTQMTRELFKVEDGDEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFVSGHN 259
           VSPHVVP+QMTR+LFK+EDGDEFPNVYSHFKCGDILR +D+AE ALYLASDASRFVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLASDASRFVSGHN 251

Query: 260 FILDGGFTAGNQGLCSYQ 278
            I+DGGFT+ NQGLCSYQ
Sbjct: 252 LIVDGGFTSANQGLCSYQ 269

BLAST of HG10021721 vs. ExPASy TrEMBL
Match: A0A6J1EID0 (secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.4e-124
Identity = 222/258 (86.05%), Postives = 241/258 (93.41%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDV 79
           RL+GKVA+ITGAARGIGESTA+ FFKHGAKVV+ADILDD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 80  TKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGT 139
           TKE D+ENA+NTAISKYGKLDIM NNAGI+GTLKFNILENE SDF+ VLNVNLLGAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 140 KHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVNC 199
           KHAAKAMIP  QGSII+ AS CSIIGGIGPYAYTSSKHGLLGLM+NA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 200 VSPHVVPTQMTRELFKVEDGDEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFVSGHN 259
           VSPHVVP+QMTR+LFK+EDGDEFPNVYSHFKCGDILR +D+AE ALYLASDASRFVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 260 FILDGGFTAGNQGLCSYQ 278
            I+DGGFT+ NQGLCSY+
Sbjct: 252 LIVDGGFTSANQGLCSYE 269

BLAST of HG10021721 vs. ExPASy TrEMBL
Match: A0A6J1EI14 (secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.4e-124
Identity = 222/258 (86.05%), Postives = 241/258 (93.41%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDV 79
           RL+GKVA+ITGAARGIGESTA+ FFKHGAKVV+ADILDD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 80  TKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGT 139
           TKE D+ENA+NTAISKYGKLDIM NNAGI+GTLKFNILENE SDF+ VLNVNLLGAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 140 KHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVNC 199
           KHAAKAMIP  QGSII+ AS CSIIGGIGPYAYTSSKHGLLGLM+NA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 200 VSPHVVPTQMTRELFKVEDGDEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFVSGHN 259
           VSPHVVP+QMTR+LFK+EDGDEFPNVYSHFKCGDILR +D+AE ALYLASDASRFVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 260 FILDGGFTAGNQGLCSYQ 278
            I+DGGFT+ NQGLCSY+
Sbjct: 252 LIVDGGFTSANQGLCSYE 269

BLAST of HG10021721 vs. ExPASy TrEMBL
Match: A0A6J1EPB9 (secoisolariciresinol dehydrogenase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 9.2e-124
Identity = 221/257 (85.99%), Postives = 240/257 (93.39%), Query Frame = 0

Query: 21  LDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDVT 80
           L+GKVA+ITGAARGIGESTA+ FFKHGAKVV+ADILDD+G SLS HL SSSSSFVHCDVT
Sbjct: 13  LEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDVT 72

Query: 81  KETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGTK 140
           KE D+ENA+NTAISKYGKLDIM NNAGI+GTLKFNILENE SDF+ VLNVNLLGAFLGTK
Sbjct: 73  KEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGTK 132

Query: 141 HAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVNCV 200
           HAAKAMIP  QGSII+ AS CSIIGGIGPYAYTSSKHGLLGLM+NA VDLGRYGIRVNCV
Sbjct: 133 HAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNCV 192

Query: 201 SPHVVPTQMTRELFKVEDGDEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFVSGHNF 260
           SPHVVP+QMTR+LFK+EDGDEFPNVYSHFKCGDILR +D+AE ALYLASDASRFVSGHN 
Sbjct: 193 SPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHNL 252

Query: 261 ILDGGFTAGNQGLCSYQ 278
           I+DGGFT+ NQGLCSY+
Sbjct: 253 IVDGGFTSANQGLCSYE 269

BLAST of HG10021721 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 3.3e-65
Identity = 130/266 (48.87%), Postives = 184/266 (69.17%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILD----DLGRSLSNHLSSSSSSFV 79
           RL+GKVAIITG A GIG++T   F +HGA VVIAD+ +     L +SLS+H +S   +F+
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 80  HCDVTKETDVENAINTAISKYGKLDIMFNNAGITGTLK--FNILENESSDFKNVLNVNLL 139
            CDV+ E DVEN +N  +++YG+LDI+FNNAG+ G  K   +IL+ ++ +F +V+ VN+ 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 140 GAFLGTKHAAKAMIPTG-QGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGR 199
           G  LG KH A+AMI  G +G II +ASV  ++GG+GP+AYT+SKH ++GL +NAA +LG+
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 200 YGIRVNCVSPHVVPTQMTRELFK-----------VEDGDEFPNVYSHFKCGDILRDKDVA 259
           YGIRVNC+SP  V T M    ++           VE+ +EF    ++ K G+ LR  D+A
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLK-GETLRANDIA 270

Query: 260 EVALYLASDASRFVSGHNFILDGGFT 268
           E ALYLASD S++V+GHN ++DGG T
Sbjct: 271 EAALYLASDESKYVNGHNLVVDGGVT 295

BLAST of HG10021721 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 8.7e-58
Identity = 125/282 (44.33%), Postives = 171/282 (60.64%), Query Frame = 0

Query: 1   MPKNDNSSILKTIDGDDDLRLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLG 60
           M  N  SS   ++      RL GKVA+ITG A GIGES  R F KHGAKV I D+ DDLG
Sbjct: 1   MSTNTESSSYSSLPSQ---RLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLG 60

Query: 61  ----RSLSNHLSSSSSSFVHCDVTKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNI 120
               +SL    S  ++ F+H DV  E D+ NA++ A+  +G LDI+ NNAG+ G    +I
Sbjct: 61  GEVCKSLLRGESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDI 120

Query: 121 LENESSDFKNVLNVNLLGAFLGTKHAAKAMIPTGQGSIIMSASVCSIIGGIGPYAYTSSK 180
                S+F+   +VN+ GAFL  KHAA+ MIP  +GSI+   SV  ++GG+GP++Y  SK
Sbjct: 121 RNYSLSEFEMTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSK 180

Query: 181 HGLLGLMRNAAVDLGRYGIRVNCVSPHVVPTQMTRELFKVEDGDE--------FPNVYSH 240
           H +LGL R+ A +LG++GIRVNCVSP+ V T++       E+  E        F    ++
Sbjct: 181 HAVLGLTRSVAAELGQHGIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANAN 240

Query: 241 FKCGDILRDKDVAEVALYLASDASRFVSGHNFILDGGFTAGN 271
            K  ++  D DVA   L+LASD SR++SG N ++DGGFT  N
Sbjct: 241 LKGVELTVD-DVANAVLFLASDDSRYISGDNLMIDGGFTCTN 278

BLAST of HG10021721 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 219.5 bits (558), Expect = 3.3e-57
Identity = 117/252 (46.43%), Postives = 171/252 (67.86%), Query Frame = 0

Query: 19  LRLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCD 78
           LRLDGK+AIITG A GIG    R F  HGAKVVI DI ++LG++L+  +    +SF  C+
Sbjct: 4   LRLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCN 63

Query: 79  VTKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLG 138
           VT ETDVENA+   + K+GKLD++F+NAG+      ++L+ +   F   + VN+ GA   
Sbjct: 64  VTDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFG-SVLDLDLEAFDRTMAVNVRGAAAF 123

Query: 139 TKHAAKAMIPTG-QGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRV 198
            KHAA++M+ +G +GSI+ + S+ + IGG GP++YT+SKH LLGL+R+A   LG+YGIRV
Sbjct: 124 IKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRV 183

Query: 199 NCVSPHVVPTQMTRELFK--VEDGDEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFV 258
           N V+P+ V T MT    +  V+  +E+     + K G +L+ + +AE AL+LASD S ++
Sbjct: 184 NGVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLK-GVVLKARHIAEAALFLASDDSVYI 243

Query: 259 SGHNFILDGGFT 268
           SG N ++DGGF+
Sbjct: 244 SGQNLVVDGGFS 253

BLAST of HG10021721 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 216.1 bits (549), Expect = 3.6e-56
Identity = 113/251 (45.02%), Postives = 168/251 (66.93%), Query Frame = 0

Query: 20  RLDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDV 79
           RLDGK+ IITG A GIG  + R F +HGA+VVI D+ D+LG++++  +    +S+ HCDV
Sbjct: 5   RLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCDV 64

Query: 80  TKETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGT 139
           T ET+VENA+   + KYGKLD++F+NAG+      +IL+   ++    + +NL G     
Sbjct: 65  TNETEVENAVKFTVEKYGKLDVLFSNAGVIEPF-VSILDLNLNELDRTIAINLRGTAAFI 124

Query: 140 KHAAKAMIPTG-QGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVN 199
           KHAA+AM+  G +GSI+ + SV + I G  P+ YT+SKHGLLGL+++A+  LG+YGIRVN
Sbjct: 125 KHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIRVN 184

Query: 200 CVSPHVVPTQMTRELFKVEDG--DEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFVS 259
            V+P  V T +    FK+E    ++  +  ++ K G +L+ + VAE AL+LASD S +VS
Sbjct: 185 GVAPFGVATPLVCNGFKMEPNVVEQNTSASANLK-GIVLKARHVAEAALFLASDESAYVS 244

Query: 260 GHNFILDGGFT 268
           G N  +DGG++
Sbjct: 245 GQNLAVDGGYS 253

BLAST of HG10021721 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 215.7 bits (548), Expect = 4.8e-56
Identity = 115/250 (46.00%), Postives = 169/250 (67.60%), Query Frame = 0

Query: 21  LDGKVAIITGAARGIGESTARHFFKHGAKVVIADILDDLGRSLSNHLSSSSSSFVHCDVT 80
           LDGK+AIITG A GIG    R F  HGAKVVI DI ++LG++L+  +    +SF  C+VT
Sbjct: 44  LDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNVT 103

Query: 81  KETDVENAINTAISKYGKLDIMFNNAGITGTLKFNILENESSDFKNVLNVNLLGAFLGTK 140
            ETDVENA+   + K+GKLD++F+NAG+      ++L+ +   F   + VN+ GA    K
Sbjct: 104 DETDVENAVKFTVEKHGKLDVLFSNAGVLEAFG-SVLDLDLEAFDRTMAVNVRGAAAFIK 163

Query: 141 HAAKAMIPTG-QGSIIMSASVCSIIGGIGPYAYTSSKHGLLGLMRNAAVDLGRYGIRVNC 200
           HAA++M+ +G +GSI+ + S+ + IGG GP++YT+SKH LLGL+R+A   LG+YGIRVN 
Sbjct: 164 HAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVNG 223

Query: 201 VSPHVVPTQMTRELFK--VEDGDEFPNVYSHFKCGDILRDKDVAEVALYLASDASRFVSG 260
           V+P+ V T MT    +  V+  +E+     + K G +L+ + +AE AL+LASD S ++SG
Sbjct: 224 VAPYGVATGMTSAYNEEAVKMLEEYGEALGNLK-GVVLKARHIAEAALFLASDDSVYISG 283

Query: 261 HNFILDGGFT 268
            N ++DGGF+
Sbjct: 284 QNLVVDGGFS 291