HG10019417 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10019417 vs. NCBI nr
Match: XP_008461801.1 (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 833.9 bits (2153), Expect = 6.2e-238
Identity = 410/445 (92.13%), Postives = 421/445 (94.61%), Query Frame = 0

Query: 1   MHQQNHLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSM 60
           MHQQN LFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIY HYPTP+SLYTFRNTDF  S 
Sbjct: 3   MHQQNRLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYNHYPTPASLYTFRNTDFHSSK 62

Query: 61  TTKMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRT 120
           TTK R+ RGSS S   KVVNVDDFGAKGNG QDD+KAFELAWKEACSSRKATLLVPKGRT
Sbjct: 63  TTKKRRDRGSSFSSSTKVVNVDDFGAKGNG-QDDTKAFELAWKEACSSRKATLLVPKGRT 122

Query: 121 YYLKPITFSGPCRAPLTLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGIINGN 180
           YYLKPITFSGPCR+PLTL INGMIKAS  +SDY+NDRRHWLKFEGVDNFVVEGNGIINGN
Sbjct: 123 YYLKPITFSGPCRSPLTLKINGMIKASTRISDYENDRRHWLKFEGVDNFVVEGNGIINGN 182

Query: 181 GRKWWQNSCKVNKEL-------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNL 240
           GRKWWQNSCKVNKEL       AVTFYQCINLVV+NLMFK+AQQMHLTFQKCTNVKALNL
Sbjct: 183 GRKWWQNSCKVNKELPCKRAPTAVTFYQCINLVVSNLMFKDAQQMHLTFQKCTNVKALNL 242

Query: 241 RVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISI 300
           RVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNV+AMDITCGPGHGISI
Sbjct: 243 RVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVRAMDITCGPGHGISI 302

Query: 301 GSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPIII 360
           GSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVM NVSNPIII
Sbjct: 303 GSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMKNVSNPIII 362

Query: 361 DQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVG 420
           DQNYCDK+DSCPEQ SAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVG
Sbjct: 363 DQNYCDKEDSCPEQNSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVG 422

Query: 421 QGDEVATASCENVRLKNRWKVYPQC 439
           QG+EVATASCENVRLKNRWKVYPQC
Sbjct: 423 QGNEVATASCENVRLKNRWKVYPQC 446

BLAST of HG10019417 vs. NCBI nr
Match: XP_038905664.1 (polygalacturonase-like [Benincasa hispida])

HSP 1 Score: 809.3 bits (2089), Expect = 1.6e-230
Identity = 405/445 (91.01%), Postives = 411/445 (92.36%), Query Frame = 0

Query: 1   MHQQNHLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSM 60
           MHQQNHLFIFFIAFSLFSLCFG+YLEDP PVSQYHDQI+ HYP         NTDF   M
Sbjct: 1   MHQQNHLFIFFIAFSLFSLCFGNYLEDPLPVSQYHDQIFSHYPR-------WNTDFHSLM 60

Query: 61  TTKMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRT 120
           TTK R  RGSSLSG  KVVNVDDFGAKGNG  DDSKAFELAW+EACSSRKATLLVPKGRT
Sbjct: 61  TTKTRY-RGSSLSGSTKVVNVDDFGAKGNG-GDDSKAFELAWREACSSRKATLLVPKGRT 120

Query: 121 YYLKPITFSGPCRAPLTLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGIINGN 180
           YYLKPITFSGPCR+PLTL INGMIKASP MSDYDNDRRHWLKFEGVDNFVVEGNGII+GN
Sbjct: 121 YYLKPITFSGPCRSPLTLKINGMIKASPRMSDYDNDRRHWLKFEGVDNFVVEGNGIIDGN 180

Query: 181 GRKWWQNSCKVNKEL-------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNL 240
           GRKWWQNSCKVNKEL       AVTFYQCINLVV NLMFKNAQQMHLTFQKCTNVKALNL
Sbjct: 181 GRKWWQNSCKVNKELPCRRAPTAVTFYQCINLVVTNLMFKNAQQMHLTFQKCTNVKALNL 240

Query: 241 RVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISI 300
           RVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISI
Sbjct: 241 RVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISI 300

Query: 301 GSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPIII 360
           GSLGA NSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVM NVSNPIII
Sbjct: 301 GSLGAGNSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMKNVSNPIII 360

Query: 361 DQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVG 420
           DQNYCDKK+SCPEQ SAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVG
Sbjct: 361 DQNYCDKKESCPEQNSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVG 420

Query: 421 QGDEVATASCENVRLKNRWKVYPQC 439
           QGDEVATASCENVRLKNRWKVYPQC
Sbjct: 421 QGDEVATASCENVRLKNRWKVYPQC 436

BLAST of HG10019417 vs. NCBI nr
Match: XP_011651773.1 (polygalacturonase [Cucumis sativus] >KGN58587.1 hypothetical protein Csa_001963 [Cucumis sativus])

HSP 1 Score: 786.2 bits (2029), Expect = 1.5e-223
Identity = 395/447 (88.37%), Postives = 408/447 (91.28%), Query Frame = 0

Query: 1   MHQQNHLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSM 60
           MHQQN LFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIY  YPTP+SLYT RNTDF  SM
Sbjct: 3   MHQQNRLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYSDYPTPASLYTSRNTDFHSSM 62

Query: 61  TTKMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRT 120
            TK R  RGSS SG  KVVNVDDFGAKGNG QDD+KAFELAWKEACSSRKATLLVPKGRT
Sbjct: 63  MTKKRPDRGSSFSGSTKVVNVDDFGAKGNG-QDDTKAFELAWKEACSSRKATLLVPKGRT 122

Query: 121 YYLKPITFSGPCRAPLTLMINGMIKASPIMSDYDND-RRHWLKFEGVDNFVVEGNGIING 180
           YYLKPI FSGPCR+PLTL INGMIKAS  +SDY+ND RRHWLKF  VDNFVVEGNGIING
Sbjct: 123 YYLKPIIFSGPCRSPLTLKINGMIKASTRISDYENDGRRHWLKFHEVDNFVVEGNGIING 182

Query: 181 NGRKWWQNSCKVNKEL-------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALN 240
           NGRKWWQNSCKVNK L       AVTFYQCINLVV+NLMFK+AQQMHLTFQKCTNVKALN
Sbjct: 183 NGRKWWQNSCKVNKGLPCKRAPTAVTFYQCINLVVSNLMFKDAQQMHLTFQKCTNVKALN 242

Query: 241 LRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGIS 300
           LRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGIS
Sbjct: 243 LRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGIS 302

Query: 301 IGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPII 360
           IGSLGA+NSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVM NVSNPII
Sbjct: 303 IGSLGADNSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMRNVSNPII 362

Query: 361 IDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIV 420
           IDQNYCDKK+SCPEQ SAVKVSNVVYKNIRGTSAS DAIKFDCSKSFPCQ ISLLG+ IV
Sbjct: 363 IDQNYCDKKESCPEQKSAVKVSNVVYKNIRGTSASGDAIKFDCSKSFPCQGISLLGIRIV 422

Query: 421 GQGDEVA-TASCENVRLKNRWKVYPQC 439
           GQG+E A TASCENVR + + KVYPQC
Sbjct: 423 GQGNEEAVTASCENVRWETKLKVYPQC 448

BLAST of HG10019417 vs. NCBI nr
Match: KAA0048359.1 (polygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 777.7 bits (2007), Expect = 5.3e-221
Identity = 387/448 (86.38%), Postives = 399/448 (89.06%), Query Frame = 0

Query: 17  FSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSMTTKMRQGRGSSLSGLQ 76
           F     +YLEDPFPVSQYHDQIY HYPTP+SLYTFRNTDF  S TTK R+ RGSS S   
Sbjct: 27  FESTHNNYLEDPFPVSQYHDQIYNHYPTPASLYTFRNTDFHSSKTTKKRRDRGSSFSSST 86

Query: 77  KVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCRAPL 136
           KVVNVDDFGAKGNG QDD+KAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCR+PL
Sbjct: 87  KVVNVDDFGAKGNG-QDDTKAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCRSPL 146

Query: 137 TLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKELA 196
           TL INGMIKAS  +SDY+NDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKELA
Sbjct: 147 TLKINGMIKASTRISDYENDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKELA 206

Query: 197 VTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHITETQNAI 256
           VTFYQCINLVV+NLMFK+AQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHITETQNAI
Sbjct: 207 VTFYQCINLVVSNLMFKDAQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHITETQNAI 266

Query: 257 IRNCVIGT--------------------------GDDCISIVSGSRNVQAMDITCGPGHG 316
           IRNCVIGT                          GDDCISIVSGSRNV+AMDITCGPGHG
Sbjct: 267 IRNCVIGTGTLNEENETTSILSLLNCLSKICFFLGDDCISIVSGSRNVRAMDITCGPGHG 326

Query: 317 ISIGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNP 376
           ISIGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQ  SGYAKNIMFQNVVM NVSNP
Sbjct: 327 ISIGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQVCSGYAKNIMFQNVVMKNVSNP 386

Query: 377 IIIDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVH 436
           IIIDQNYCDK+DSCPEQ SAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVH
Sbjct: 387 IIIDQNYCDKEDSCPEQNSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVH 446

Query: 437 IVGQGDEVATASCENVRLKNRWKVYPQC 439
           IVGQG+EVATASCENVRLKNRWKVYPQC
Sbjct: 447 IVGQGNEVATASCENVRLKNRWKVYPQC 473

BLAST of HG10019417 vs. NCBI nr
Match: TYK01108.1 (polygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 772.7 bits (1994), Expect = 1.7e-219
Identity = 389/468 (83.12%), Postives = 401/468 (85.68%), Query Frame = 0

Query: 17  FSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSMTTKMRQGRGSSLSGLQ 76
           F     +YLEDPFPVSQYHDQIY HYPTP+SLYTFRNTDF  S TTK R+ RGSS S   
Sbjct: 27  FESTHNNYLEDPFPVSQYHDQIYNHYPTPASLYTFRNTDFHSSKTTKKRRDRGSSFSSST 86

Query: 77  KVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCRAPL 136
           KVVNVDDFGAKGNG QDD+KAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCR+PL
Sbjct: 87  KVVNVDDFGAKGNG-QDDTKAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCRSPL 146

Query: 137 TLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKEL- 196
           TL INGMIKAS  +SDY+NDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKEL 
Sbjct: 147 TLKINGMIKASTRISDYENDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKELP 206

Query: 197 ------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHI 256
                 AVTFYQCINLVV+NLMFK+AQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHI
Sbjct: 207 CKRAPTAVTFYQCINLVVSNLMFKDAQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHI 266

Query: 257 TETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISIGSLGAENSEAEVSNIR 316
           TETQNAIIRNCVIGTGDDCISIVSGSRNV+AMDITCGPGHGISIGSLGAENSEAEVSNIR
Sbjct: 267 TETQNAIIRNCVIGTGDDCISIVSGSRNVRAMDITCGPGHGISIGSLGAENSEAEVSNIR 326

Query: 317 VNRALISGTSNGVRIKTWQ---------------------------------------GG 376
           VNRALISGTSNGVRIKTWQ                                       GG
Sbjct: 327 VNRALISGTSNGVRIKTWQLNSQYLTEKSWPHFQTAVDPLNFNPTIPTPPPANLHDLHGG 386

Query: 377 SGYAKNIMFQNVVMNNVSNPIIIDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDA 436
           SGYAKNIMFQNVVM NVSNPIIIDQNYCDK+DSCPEQ SAVKVSNVVYKNIRGTSASDDA
Sbjct: 387 SGYAKNIMFQNVVMKNVSNPIIIDQNYCDKEDSCPEQNSAVKVSNVVYKNIRGTSASDDA 446

Query: 437 IKFDCSKSFPCQEISLLGVHIVGQGDEVATASCENVRLKNRWKVYPQC 439
           IKFDCSKSFPCQEISLLGVHIVGQG+EVATASCENVRLKNRWKVYPQC
Sbjct: 447 IKFDCSKSFPCQEISLLGVHIVGQGNEVATASCENVRLKNRWKVYPQC 493

BLAST of HG10019417 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.6e-127
Identity = 255/459 (55.56%), Postives = 304/459 (66.23%), Query Frame = 0

Query: 1   MHQQNHLFIFFIAFSL---FSLCFGSYLEDPFPVSQYHDQIYGH--YPTPSSLYTFRNTD 60
           M  Q   F F I   L   F L + S + +  P   YH + YG+     PS + T  + D
Sbjct: 1   MALQRRFFQFVIITLLIPSFILGYTSAVHED-PPHDYHLEEYGYDFKAYPSYITTIGDND 60

Query: 61  FPGSMT--------TKMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACS 120
           F  SM+         K+  G    L    K VNVDDFGAKG+G +DD+KAFE AWK ACS
Sbjct: 61  FGSSMSHENGIFGLRKVDYGMDRVLDA-SKTVNVDDFGAKGDG-RDDTKAFEKAWKAACS 120

Query: 121 S-RKATLLVPKGRTYYLKPITFSGPCRAPLTLMINGMIKASPIMSDYDNDRRHWLKFEGV 180
           S   A LLVPK + Y ++PI+FSGPC++ LT+ I G I+AS   SDY  D RHWL F+ V
Sbjct: 121 STSSAVLLVPK-KNYLVRPISFSGPCKSGLTMQIYGTIEASDDRSDYRKDGRHWLVFDSV 180

Query: 181 DNFVVEGNGIINGNGRKWWQNSCKVNKEL-------AVTFYQCINLVVANLMFKNAQQMH 240
            N  VEG G INGNG+ WWQNSCK NK L       A+TFY+  +++V NL  +NAQQ+H
Sbjct: 181 QNLRVEGGGTINGNGKIWWQNSCKTNKALPCKDAPTALTFYKSKHVIVKNLKIENAQQIH 240

Query: 241 LTFQKCTNVKALNLRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQ 300
           ++F  C NV+A NL V AP NSPNTDGIH+T TQN  I +CVIGTGDDCISIV+GSR V+
Sbjct: 241 VSFDNCVNVQASNLMVTAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVR 300

Query: 301 AMDITCGPGHGISIGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMF 360
             DITCGPGHGISIGSLG  NSEA VS++ VN A + GT+NGVRIKTWQGGSG A NI F
Sbjct: 301 VNDITCGPGHGISIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKF 360

Query: 361 QNVVMNNVSNPIIIDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSF 420
           QNV M+NV NPIIIDQNYCD+   C EQ+SAV+V NVVY+NI+GT AS+ AI FDCSK F
Sbjct: 361 QNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRF 420

Query: 421 PCQEISLLGVHIVGQGDEVATASCENVRLKNRWKVYPQC 439
           PCQ I L  V +  +G   A A C NV L     V P C
Sbjct: 421 PCQGIVLEDVDLEIEGGAAAKALCNNVELSETGVVSPHC 455

BLAST of HG10019417 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 431.4 bits (1108), Expect = 1.2e-119
Identity = 234/445 (52.58%), Postives = 301/445 (67.64%), Query Frame = 0

Query: 7   LFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSMTTK--- 66
           L +F + F     CF SY   PF    + D ++      S++Y   N        T+   
Sbjct: 8   LSVFLLTF--LPSCFSSY---PF---NHRDDLF----MSSNVYYETNRQHQHGHNTRNSH 67

Query: 67  MRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLV-PKGRTYY 126
           ++   G +     +  NV+ FGAK NG  DDSKAF  AW+ ACSS     +V PK R Y 
Sbjct: 68  LKNRHGYAPRSSPRSFNVNTFGAKANG-NDDSKAFMKAWEAACSSTGIVYIVAPKNRDYM 127

Query: 127 LKPITFSGPCRAPLTLM-INGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGIINGNG 186
           LK +TFSGPC++ L +  I G I+A    SDY  +RRHW+ FE V+N  VEG G I+GNG
Sbjct: 128 LKAVTFSGPCKSSLIIFKIYGRIEAWENPSDY-KERRHWIVFENVNNLRVEGGGRIDGNG 187

Query: 187 RKWWQNSCKVNKEL-------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNLR 246
             WW  SCK+N +L       AVTF +C NL V+N+  +NAQQMHLTFQ C NVKALNL 
Sbjct: 188 HIWWPKSCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLM 247

Query: 247 VVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISIG 306
           V +P +SPNTDGIH++ TQN +I++ ++ TGDDCISIVSGS NV+A  ITCGPGHGISIG
Sbjct: 248 VTSPADSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIG 307

Query: 307 SLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPIIID 366
           SLG +NSEA VSN+ VN+A + GT+NGVRIKTWQGG G AKNI+FQ+++M NV+NPIII+
Sbjct: 308 SLGEDNSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIIN 367

Query: 367 QNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVGQ 426
           Q+YCD+ ++CPEQ SAV+VSNV+YKNI+GTS+   A+KF CSK+ PC+ IS+  V +V Q
Sbjct: 368 QDYCDRVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQ 427

Query: 427 -GDEVATASCENVRLKNRWKVYPQC 439
              +V+ ASC NV+L  R  V P C
Sbjct: 428 TQQDVSKASCSNVKLDTRGNVSPLC 438

BLAST of HG10019417 vs. ExPASy Swiss-Prot
Match: P05117 (Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 2.3e-118
Identity = 228/448 (50.89%), Postives = 293/448 (65.40%), Query Frame = 0

Query: 3   QQNHLFIFFIAF-SLFSLCFGSYLEDPFPVSQYH---DQIYGHYPTPSSLYTFRNTDFPG 62
           Q+N + +  I F S  S C  + ++D      Y    +Q + H       Y  +N +   
Sbjct: 4   QRNSILLLIIIFASSISTCRSNVIDDNLFKQVYDNILEQEFAHDFQAYLSYLSKNIESNN 63

Query: 63  SMTTKMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSR-KATLLVPK 122
           ++    + G         KV+NV  FGAKG+G+  D+ AFE AW EACSSR     +VPK
Sbjct: 64  NIDKVDKNG--------IKVINVLSFGAKGDGKTYDNIAFEQAWNEACSSRTPVQFVVPK 123

Query: 123 GRTYYLKPITFSGPCRAPLTLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGII 182
            + Y LK ITFSGPCR+ +++ I G ++AS  +SDY  DRR W+ F+ V N VV G G I
Sbjct: 124 NKNYLLKQITFSGPCRSSISVKIFGSLEASSKISDY-KDRRLWIAFDSVQNLVVGGGGTI 183

Query: 183 NGNGRKWWQNSCKVNKEL-------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKA 242
           NGNG+ WW +SCK+NK L       A+TF+ C NL V NL  KNAQQ+H+ F+ CTNV A
Sbjct: 184 NGNGQVWWPSSCKINKSLPCRDAPTALTFWNCKNLKVNNLKSKNAQQIHIKFESCTNVVA 243

Query: 243 LNLRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHG 302
            NL + A   SPNTDG+H++ TQ   I + +IGTGDDCISIVSGS+NVQA +ITCGPGHG
Sbjct: 244 SNLMINASAKSPNTDGVHVSNTQYIQISDTIIGTGDDCISIVSGSQNVQATNITCGPGHG 303

Query: 303 ISIGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNP 362
           ISIGSLG+ NSEA VSN+ VN A I G  NGVRIKTWQGGSG A NI F NV M +V  P
Sbjct: 304 ISIGSLGSGNSEAYVSNVTVNEAKIIGAENGVRIKTWQGGSGQASNIKFLNVEMQDVKYP 363

Query: 363 IIIDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVH 422
           IIIDQNYCD+ + C +Q SAV+V NVVY+NI+GTSA+  AIKFDCS +FPC+ I +  ++
Sbjct: 364 IIIDQNYCDRVEPCIQQFSAVQVKNVVYENIKGTSATKVAIKFDCSTNFPCEGIIMENIN 423

Query: 423 IVGQGDEVATASCENVRLKNRWKVYPQC 439
           +VG+  + + A+C+NV   N   V P C
Sbjct: 424 LVGESGKPSEATCKNVHFNNAEHVTPHC 442

BLAST of HG10019417 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 1.6e-116
Identity = 210/356 (58.99%), Postives = 262/356 (73.60%), Query Frame = 0

Query: 79  VNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCRAPLTL 138
           ++VDDFGA+G+G  DD+KAFE AWK+ACSS  + L+VP+ + Y LK ITFSGPC++ L +
Sbjct: 85  ISVDDFGARGDG-TDDTKAFEKAWKDACSS-GSVLIVPENKNYLLKQITFSGPCKSDLRV 144

Query: 139 MINGMIKASPIMSDY-DNDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKEL-- 198
            I G I+AS   SD+  ++R+ W++FE + N  +EG G  NGNG  WW +SCK  K L  
Sbjct: 145 KIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWDSSCKRKKSLPC 204

Query: 199 -----AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHIT 258
                A+TF  C NL+V++L  K++Q+MHL+F KC +V A NL V AP +SPNTDGIHIT
Sbjct: 205 KSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSPNTDGIHIT 264

Query: 259 ETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISIGSLGAENSEAEVSNIRV 318
            TQ   + N VIGTGDDCISI SGS+ V A +ITCGPGHGISIGSLG  NSEA VS + V
Sbjct: 265 GTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSEAHVSGVLV 324

Query: 319 NRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPIIIDQNYCDKKDSCPEQTSA 378
           +   +  T+NG+RIKTWQGGSG AKNI FQN+VM+NV+NPIIIDQ YCD KD CPEQ SA
Sbjct: 325 DGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKDPCPEQESA 384

Query: 379 VKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVGQGDEVATASCENV 427
           VKVSNV Y NIRGTSAS+ A+KFDCSKS PCQ   +  +++VG G +  T SC N+
Sbjct: 385 VKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGKETTMSCSNI 438

BLAST of HG10019417 vs. ExPASy Swiss-Prot
Match: P48978 (Polygalacturonase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.8e-113
Identity = 233/455 (51.21%), Postives = 287/455 (63.08%), Query Frame = 0

Query: 9   IFFIAFSLFSLCFGSYLEDPFPVSQYHDQI------YGHYPTPSSLYTFR---------- 68
           +F ++FS  S C GS  ++   +  Y D +      Y     PS   T            
Sbjct: 14  VFVVSFSTTS-CSGSSFQEVNALHSYVDHVDDKESGYNSRAYPSYTDTIEGLKVMELIRP 73

Query: 69  NTDFPGSMTTKMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKAT 128
            T    S       G  ++ S   K ++VDDFGAKGNG  DD++AF  AWK ACSS  A 
Sbjct: 74  RTQLFSSRKLNTITGGIATSSAPAKTISVDDFGAKGNG-ADDTQAFVKAWKAACSSSGAM 133

Query: 129 LLVPKGRTYYLKPITFSGPCRAPLTLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVE 188
           +LV   + Y ++PI FSGPC++ LTL I G I+AS   S Y  D  HWL F+ V N +V 
Sbjct: 134 VLVVPQKNYLVRPIEFSGPCKSQLTLQIYGTIEASEDRSIY-KDIDHWLIFDNVQNLLVV 193

Query: 189 GNGIINGNGRKWWQNSCKVNKE--------LAVTFYQCINLVVANLMFKNAQQMHLTFQK 248
           G G INGNG  WW+NSCK+  +         AVTF +C NLVV NL  ++AQQ+H+ FQ 
Sbjct: 194 GPGTINGNGNIWWKNSCKIKPQPPCGTYAPTAVTFNRCNNLVVKNLNIQDAQQIHVIFQN 253

Query: 249 CTNVKALNLRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDIT 308
           C NV+A  L V AP +SPNTDGIH+T TQN  I + VIGTGDDCISIVSGS+ VQA DIT
Sbjct: 254 CINVQASCLTVTAPEDSPNTDGIHVTNTQNITISSSVIGTGDDCISIVSGSQRVQATDIT 313

Query: 309 CGPGHGISIGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVM 368
           CGPGHGISIGSLG + SE  VS + VN A +SGTSNG+RIKTW+GGSG A NI+FQNV M
Sbjct: 314 CGPGHGISIGSLGEDGSEDHVSGVFVNGAKLSGTSNGLRIKTWKGGSGSATNIVFQNVQM 373

Query: 369 NNVSNPIIIDQNYCD-KKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQE 428
           N+V+NPIIIDQNYCD K   C +Q SAV+V NV+Y+NIRGTSAS DAI  +CS+S PCQ 
Sbjct: 374 NDVTNPIIIDQNYCDHKTKDCKQQKSAVQVKNVLYQNIRGTSASGDAITLNCSQSVPCQG 433

Query: 429 ISLLGVHIVGQGDEVATASCENVRLKNRWKVYPQC 439
           I L  V +     +   A C NV+   +  V P+C
Sbjct: 434 IVLQSVQL-----QNGRAECNNVQPAYKGVVSPRC 460

BLAST of HG10019417 vs. ExPASy TrEMBL
Match: A0A1S3CG06 (polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103500316 PE=3 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.0e-238
Identity = 410/445 (92.13%), Postives = 421/445 (94.61%), Query Frame = 0

Query: 1   MHQQNHLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSM 60
           MHQQN LFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIY HYPTP+SLYTFRNTDF  S 
Sbjct: 3   MHQQNRLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYNHYPTPASLYTFRNTDFHSSK 62

Query: 61  TTKMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRT 120
           TTK R+ RGSS S   KVVNVDDFGAKGNG QDD+KAFELAWKEACSSRKATLLVPKGRT
Sbjct: 63  TTKKRRDRGSSFSSSTKVVNVDDFGAKGNG-QDDTKAFELAWKEACSSRKATLLVPKGRT 122

Query: 121 YYLKPITFSGPCRAPLTLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGIINGN 180
           YYLKPITFSGPCR+PLTL INGMIKAS  +SDY+NDRRHWLKFEGVDNFVVEGNGIINGN
Sbjct: 123 YYLKPITFSGPCRSPLTLKINGMIKASTRISDYENDRRHWLKFEGVDNFVVEGNGIINGN 182

Query: 181 GRKWWQNSCKVNKEL-------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNL 240
           GRKWWQNSCKVNKEL       AVTFYQCINLVV+NLMFK+AQQMHLTFQKCTNVKALNL
Sbjct: 183 GRKWWQNSCKVNKELPCKRAPTAVTFYQCINLVVSNLMFKDAQQMHLTFQKCTNVKALNL 242

Query: 241 RVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISI 300
           RVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNV+AMDITCGPGHGISI
Sbjct: 243 RVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVRAMDITCGPGHGISI 302

Query: 301 GSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPIII 360
           GSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVM NVSNPIII
Sbjct: 303 GSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMKNVSNPIII 362

Query: 361 DQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVG 420
           DQNYCDK+DSCPEQ SAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVG
Sbjct: 363 DQNYCDKEDSCPEQNSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVG 422

Query: 421 QGDEVATASCENVRLKNRWKVYPQC 439
           QG+EVATASCENVRLKNRWKVYPQC
Sbjct: 423 QGNEVATASCENVRLKNRWKVYPQC 446

BLAST of HG10019417 vs. ExPASy TrEMBL
Match: A0A0A0LD18 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G690840 PE=3 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 7.2e-224
Identity = 395/447 (88.37%), Postives = 408/447 (91.28%), Query Frame = 0

Query: 1   MHQQNHLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSM 60
           MHQQN LFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIY  YPTP+SLYT RNTDF  SM
Sbjct: 3   MHQQNRLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYSDYPTPASLYTSRNTDFHSSM 62

Query: 61  TTKMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRT 120
            TK R  RGSS SG  KVVNVDDFGAKGNG QDD+KAFELAWKEACSSRKATLLVPKGRT
Sbjct: 63  MTKKRPDRGSSFSGSTKVVNVDDFGAKGNG-QDDTKAFELAWKEACSSRKATLLVPKGRT 122

Query: 121 YYLKPITFSGPCRAPLTLMINGMIKASPIMSDYDND-RRHWLKFEGVDNFVVEGNGIING 180
           YYLKPI FSGPCR+PLTL INGMIKAS  +SDY+ND RRHWLKF  VDNFVVEGNGIING
Sbjct: 123 YYLKPIIFSGPCRSPLTLKINGMIKASTRISDYENDGRRHWLKFHEVDNFVVEGNGIING 182

Query: 181 NGRKWWQNSCKVNKEL-------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALN 240
           NGRKWWQNSCKVNK L       AVTFYQCINLVV+NLMFK+AQQMHLTFQKCTNVKALN
Sbjct: 183 NGRKWWQNSCKVNKGLPCKRAPTAVTFYQCINLVVSNLMFKDAQQMHLTFQKCTNVKALN 242

Query: 241 LRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGIS 300
           LRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGIS
Sbjct: 243 LRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGIS 302

Query: 301 IGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPII 360
           IGSLGA+NSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVM NVSNPII
Sbjct: 303 IGSLGADNSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMRNVSNPII 362

Query: 361 IDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIV 420
           IDQNYCDKK+SCPEQ SAVKVSNVVYKNIRGTSAS DAIKFDCSKSFPCQ ISLLG+ IV
Sbjct: 363 IDQNYCDKKESCPEQKSAVKVSNVVYKNIRGTSASGDAIKFDCSKSFPCQGISLLGIRIV 422

Query: 421 GQGDEVA-TASCENVRLKNRWKVYPQC 439
           GQG+E A TASCENVR + + KVYPQC
Sbjct: 423 GQGNEEAVTASCENVRWETKLKVYPQC 448

BLAST of HG10019417 vs. ExPASy TrEMBL
Match: A0A5A7U4J0 (Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G00780 PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 2.5e-221
Identity = 387/448 (86.38%), Postives = 399/448 (89.06%), Query Frame = 0

Query: 17  FSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSMTTKMRQGRGSSLSGLQ 76
           F     +YLEDPFPVSQYHDQIY HYPTP+SLYTFRNTDF  S TTK R+ RGSS S   
Sbjct: 27  FESTHNNYLEDPFPVSQYHDQIYNHYPTPASLYTFRNTDFHSSKTTKKRRDRGSSFSSST 86

Query: 77  KVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCRAPL 136
           KVVNVDDFGAKGNG QDD+KAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCR+PL
Sbjct: 87  KVVNVDDFGAKGNG-QDDTKAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCRSPL 146

Query: 137 TLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKELA 196
           TL INGMIKAS  +SDY+NDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKELA
Sbjct: 147 TLKINGMIKASTRISDYENDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKELA 206

Query: 197 VTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHITETQNAI 256
           VTFYQCINLVV+NLMFK+AQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHITETQNAI
Sbjct: 207 VTFYQCINLVVSNLMFKDAQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHITETQNAI 266

Query: 257 IRNCVIGT--------------------------GDDCISIVSGSRNVQAMDITCGPGHG 316
           IRNCVIGT                          GDDCISIVSGSRNV+AMDITCGPGHG
Sbjct: 267 IRNCVIGTGTLNEENETTSILSLLNCLSKICFFLGDDCISIVSGSRNVRAMDITCGPGHG 326

Query: 317 ISIGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNP 376
           ISIGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQ  SGYAKNIMFQNVVM NVSNP
Sbjct: 327 ISIGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQVCSGYAKNIMFQNVVMKNVSNP 386

Query: 377 IIIDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVH 436
           IIIDQNYCDK+DSCPEQ SAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVH
Sbjct: 387 IIIDQNYCDKEDSCPEQNSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVH 446

Query: 437 IVGQGDEVATASCENVRLKNRWKVYPQC 439
           IVGQG+EVATASCENVRLKNRWKVYPQC
Sbjct: 447 IVGQGNEVATASCENVRLKNRWKVYPQC 473

BLAST of HG10019417 vs. ExPASy TrEMBL
Match: A0A5D3BPX8 (Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold612G00020 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 8.2e-220
Identity = 389/468 (83.12%), Postives = 401/468 (85.68%), Query Frame = 0

Query: 17  FSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSMTTKMRQGRGSSLSGLQ 76
           F     +YLEDPFPVSQYHDQIY HYPTP+SLYTFRNTDF  S TTK R+ RGSS S   
Sbjct: 27  FESTHNNYLEDPFPVSQYHDQIYNHYPTPASLYTFRNTDFHSSKTTKKRRDRGSSFSSST 86

Query: 77  KVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCRAPL 136
           KVVNVDDFGAKGNG QDD+KAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCR+PL
Sbjct: 87  KVVNVDDFGAKGNG-QDDTKAFELAWKEACSSRKATLLVPKGRTYYLKPITFSGPCRSPL 146

Query: 137 TLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKEL- 196
           TL INGMIKAS  +SDY+NDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKEL 
Sbjct: 147 TLKINGMIKASTRISDYENDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNSCKVNKELP 206

Query: 197 ------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHI 256
                 AVTFYQCINLVV+NLMFK+AQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHI
Sbjct: 207 CKRAPTAVTFYQCINLVVSNLMFKDAQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHI 266

Query: 257 TETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISIGSLGAENSEAEVSNIR 316
           TETQNAIIRNCVIGTGDDCISIVSGSRNV+AMDITCGPGHGISIGSLGAENSEAEVSNIR
Sbjct: 267 TETQNAIIRNCVIGTGDDCISIVSGSRNVRAMDITCGPGHGISIGSLGAENSEAEVSNIR 326

Query: 317 VNRALISGTSNGVRIKTWQ---------------------------------------GG 376
           VNRALISGTSNGVRIKTWQ                                       GG
Sbjct: 327 VNRALISGTSNGVRIKTWQLNSQYLTEKSWPHFQTAVDPLNFNPTIPTPPPANLHDLHGG 386

Query: 377 SGYAKNIMFQNVVMNNVSNPIIIDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDA 436
           SGYAKNIMFQNVVM NVSNPIIIDQNYCDK+DSCPEQ SAVKVSNVVYKNIRGTSASDDA
Sbjct: 387 SGYAKNIMFQNVVMKNVSNPIIIDQNYCDKEDSCPEQNSAVKVSNVVYKNIRGTSASDDA 446

Query: 437 IKFDCSKSFPCQEISLLGVHIVGQGDEVATASCENVRLKNRWKVYPQC 439
           IKFDCSKSFPCQEISLLGVHIVGQG+EVATASCENVRLKNRWKVYPQC
Sbjct: 447 IKFDCSKSFPCQEISLLGVHIVGQGNEVATASCENVRLKNRWKVYPQC 493

BLAST of HG10019417 vs. ExPASy TrEMBL
Match: A0A6J1J4X9 (polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111481403 PE=3 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.5e-216
Identity = 379/446 (84.98%), Postives = 398/446 (89.24%), Query Frame = 0

Query: 1   MHQQNHLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGS- 60
           MHQ  H  IFFI+F LFSLCFGS+ E P PVSQYHD IYGHYP P       NT F GS 
Sbjct: 1   MHQPTHFLIFFISFPLFSLCFGSFHEYPLPVSQYHDHIYGHYPKPR-----WNTGFYGST 60

Query: 61  MTTKMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGR 120
           MTT  RQ R SSLSG  K+VNVDDFGAKGNG QDDSKAFELAWKEACSS KA +LVPKGR
Sbjct: 61  MTTTTRQNRVSSLSGSPKIVNVDDFGAKGNG-QDDSKAFELAWKEACSSSKAMILVPKGR 120

Query: 121 TYYLKPITFSGPCRAPLTLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGIING 180
           TYYLKPITFSGPC +PLTL I+GMIKASP MSDYD+DRRHWLKFEGVDNFVVEGNGIING
Sbjct: 121 TYYLKPITFSGPCSSPLTLKIDGMIKASPRMSDYDDDRRHWLKFEGVDNFVVEGNGIING 180

Query: 181 NGRKWWQNSCKVNKEL-------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALN 240
           NGRKWWQNSCKVNKEL       AVTFYQCINLVVANL  +NAQQMHLTFQKCTNVKALN
Sbjct: 181 NGRKWWQNSCKVNKELPCRGAPTAVTFYQCINLVVANLKLENAQQMHLTFQKCTNVKALN 240

Query: 241 LRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGIS 300
           LRVVAP NSPNTDGIH+TETQN IIRNCVIGTGDDCISIVSGSRNV+A+DITCGPGHGIS
Sbjct: 241 LRVVAPENSPNTDGIHVTETQNVIIRNCVIGTGDDCISIVSGSRNVRAVDITCGPGHGIS 300

Query: 301 IGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPII 360
           IGSLGA NSEAEVSNIRV+RALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNV NPII
Sbjct: 301 IGSLGAGNSEAEVSNIRVDRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVRNPII 360

Query: 361 IDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIV 420
           IDQNYCDK++SCPEQ SAVKVSNVVYKNIRGTSASDDAI+FDCSKS PCQ+IS+LGVH+V
Sbjct: 361 IDQNYCDKEESCPEQNSAVKVSNVVYKNIRGTSASDDAIRFDCSKSSPCQDISMLGVHLV 420

Query: 421 GQGDEVATASCENVRLKNRWKVYPQC 439
            QGD+VATASCENVRLKNRWKVYPQC
Sbjct: 421 RQGDDVATASCENVRLKNRWKVYPQC 440

BLAST of HG10019417 vs. TAIR 10
Match: AT3G07970.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 431.4 bits (1108), Expect = 8.6e-121
Identity = 234/445 (52.58%), Postives = 301/445 (67.64%), Query Frame = 0

Query: 7   LFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYGHYPTPSSLYTFRNTDFPGSMTTK--- 66
           L +F + F     CF SY   PF    + D ++      S++Y   N        T+   
Sbjct: 8   LSVFLLTF--LPSCFSSY---PF---NHRDDLF----MSSNVYYETNRQHQHGHNTRNSH 67

Query: 67  MRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLV-PKGRTYY 126
           ++   G +     +  NV+ FGAK NG  DDSKAF  AW+ ACSS     +V PK R Y 
Sbjct: 68  LKNRHGYAPRSSPRSFNVNTFGAKANG-NDDSKAFMKAWEAACSSTGIVYIVAPKNRDYM 127

Query: 127 LKPITFSGPCRAPLTLM-INGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEGNGIINGNG 186
           LK +TFSGPC++ L +  I G I+A    SDY  +RRHW+ FE V+N  VEG G I+GNG
Sbjct: 128 LKAVTFSGPCKSSLIIFKIYGRIEAWENPSDY-KERRHWIVFENVNNLRVEGGGRIDGNG 187

Query: 187 RKWWQNSCKVNKEL-------AVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNLR 246
             WW  SCK+N +L       AVTF +C NL V+N+  +NAQQMHLTFQ C NVKALNL 
Sbjct: 188 HIWWPKSCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLM 247

Query: 247 VVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISIG 306
           V +P +SPNTDGIH++ TQN +I++ ++ TGDDCISIVSGS NV+A  ITCGPGHGISIG
Sbjct: 248 VTSPADSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIG 307

Query: 307 SLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPIIID 366
           SLG +NSEA VSN+ VN+A + GT+NGVRIKTWQGG G AKNI+FQ+++M NV+NPIII+
Sbjct: 308 SLGEDNSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIIN 367

Query: 367 QNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVGQ 426
           Q+YCD+ ++CPEQ SAV+VSNV+YKNI+GTS+   A+KF CSK+ PC+ IS+  V +V Q
Sbjct: 368 QDYCDRVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQ 427

Query: 427 -GDEVATASCENVRLKNRWKVYPQC 439
              +V+ ASC NV+L  R  V P C
Sbjct: 428 TQQDVSKASCSNVKLDTRGNVSPLC 438

BLAST of HG10019417 vs. TAIR 10
Match: AT2G41850.1 (polygalacturonase abscission zone A. thaliana )

HSP 1 Score: 402.9 bits (1034), Expect = 3.3e-112
Identity = 208/370 (56.22%), Postives = 264/370 (71.35%), Query Frame = 0

Query: 79  VNVDDFGAKGNGQQDDSKAFELAWKEACSSRKA-TLLVPKGRTYYLKPITFSGPCRAPLT 138
           V+V DFGAKG+G+ DD++AF  AWK+ACSS  A  LLVPKG TY LK I  +GPC + LT
Sbjct: 68  VSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILT 127

Query: 139 LMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEG--NGIINGNGRKWWQNSCKVNK-- 198
           + I G + AS   SDY  D   W+ F+GV+N  V+G   G+++GNG  WWQNSCK NK  
Sbjct: 128 VQIFGTLSASQKRSDY-KDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRNKAK 187

Query: 199 -----ELAVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNLRVVAPGNSPNTDGIH 258
                  A+TFY   +L+V NL  +NAQQ+ ++ +KC+NV+  N+ V AP +SPNTDGIH
Sbjct: 188 PCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGIH 247

Query: 259 ITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISIGSLGAENSEAEVSNI 318
           IT TQN  +   +IGTGDDCISI SGS+NVQ  DITCGPGHGISIGSLG +NS+A VS +
Sbjct: 248 ITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSGV 307

Query: 319 RVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPIIIDQNYCDKKDSCPEQT 378
            V+ A +SGT NGVRIKT+QGGSG A NI+FQN+ M+NV NPIIIDQ+YCD K  C  + 
Sbjct: 308 TVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCD-KSKCTTEK 367

Query: 379 SAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVGQGDEVATASCENVRL 438
           SAV+V NVVY++I GTSAS++AI F+CSK++PCQ I L  V+I G       A+C N  +
Sbjct: 368 SAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIKG-----GKATCTNANV 427

BLAST of HG10019417 vs. TAIR 10
Match: AT3G57510.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 402.1 bits (1032), Expect = 5.6e-112
Identity = 223/446 (50.00%), Postives = 281/446 (63.00%), Query Frame = 0

Query: 6   HLFIFFIAFSLFSLCFGSYLEDPFPVSQYHDQIYGHYP---TPSSLYTFRNTDFPGSMTT 65
           HL +F     + SLC          +S   D  YGH        SL    N D    +++
Sbjct: 7   HLAVFLCVLLMLSLCKA--------LSSNVDDGYGHEDGSFESDSLLKLNNDDVLSLISS 66

Query: 66  KMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSR-KATLLVPKGRTY 125
                  S+       V+V +FGAKG+G+ DD++AF+ AWK+ACS+    T LVPKG+TY
Sbjct: 67  DETTLEAST-------VSVSNFGAKGDGKTDDTQAFKKAWKKACSTNGVTTFLVPKGKTY 126

Query: 126 YLKPITFSGPCRAPLTLMINGMIKASPIMSDYDNDRRHWLKFEGVDNFVVEG--NGIING 185
            LK   F GPC++     I G + AS   SDY  D+ HWL  E V+N  ++G   GIING
Sbjct: 127 LLKSTRFRGPCKSLRNFQILGTLSASTKRSDY-KDKNHWLILEDVNNLSIDGGSTGIING 186

Query: 186 NGRKWWQNSCKVNKE-------LAVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALN 245
           NG+ WWQNSCK++K         A+T Y   NL V NL  KNAQQ+ ++ +KC  V+  N
Sbjct: 187 NGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSN 246

Query: 246 LRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGIS 305
           + + APG+SPNTDGIHIT TQN  + N  IGTGDDCISI  G++N+Q  D+TCGPGHGIS
Sbjct: 247 VEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGIS 306

Query: 306 IGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPII 365
           IGSLG +NS+A VS I V+ A  S + NGVRIKT+QGGSG AKNI FQN+ M NV NPII
Sbjct: 307 IGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPII 366

Query: 366 IDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIV 425
           IDQ+YCD KD C +Q SAV+V NVVYKNI GTSA+D AI  +CS+ +PCQ I L  V I 
Sbjct: 367 IDQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKIK 426

Query: 426 GQGDEVATASCENVRLKNRWKVYPQC 439
           G      TASC+N  +KN+  V P+C
Sbjct: 427 G-----GTASCKNANVKNQGTVSPKC 430

BLAST of HG10019417 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 356.7 bits (914), Expect = 2.7e-98
Identity = 187/401 (46.63%), Postives = 249/401 (62.09%), Query Frame = 0

Query: 46  SSLYTFRNTDFPGSMTTKMRQGRGSSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEA 105
           SSL    N +   S+    R+   S+    +++++V +FGAKGNG  DD+KAF  AWK A
Sbjct: 20  SSLALTANANSFESLLQLPRRQSRSTRPRSERLLHVGNFGAKGNGVTDDTKAFADAWKTA 79

Query: 106 CSSR-KATLLVPKGRTYYLKPITFSGPCRAPLTLMINGMIKASPIMSDYDN-DRRHWLKF 165
           CSS+ K  +LVP+  T  L+PI  SGPC+A LTL I+G I A      ++  +RR WL F
Sbjct: 80  CSSKVKTRILVPENYTCLLRPIDLSGPCKARLTLQISGTIIAPNDPDVWEGLNRRKWLYF 139

Query: 166 EGVDNFVVEGNGIINGNGRKWWQNSCKVNKE-------LAVTFYQCINLVVANLMFKNAQ 225
            G+    VEG G +NG G++WW+ SCK N          A+TF++C N+ V NL   ++Q
Sbjct: 140 HGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQ 199

Query: 226 QMHLTFQKCTNVKALNLRVVAPGNSPNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSR 285
           QMH+    C  V    L+V+AP  SPNTDGIHI+ ++  +I N  + TGDDCISIV  S 
Sbjct: 200 QMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNST 259

Query: 286 NVQAMDITCGPGHGISIGSLGAENSEAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKN 345
            +   +I CGPGHGISIGSLG   S  EV +I V+ A+IS T+NGVRIKTWQGGSG    
Sbjct: 260 QISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSK 319

Query: 346 IMFQNVVMNNVSNPIIIDQNYCDKKDSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCS 405
           I+F+N+ MNNVSNPIIIDQ YCD +  C  QTSA+ + N+ + ++RGTSAS +AIK  CS
Sbjct: 320 IIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGTSASKEAIKISCS 379

Query: 406 KSFPCQEISLLGVHI-VGQGDEVATASCENVRLKNRWKVYP 437
            S PC+ I L  + +    GD    + C      +  +VYP
Sbjct: 380 DSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYP 420

BLAST of HG10019417 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 339.7 bits (870), Expect = 3.4e-93
Identity = 167/357 (46.78%), Postives = 231/357 (64.71%), Query Frame = 0

Query: 70  SSLSGLQKVVNVDDFGAKGNGQQDDSKAFELAWKEACSSRKATLLVPKGRTYYLKPITFS 129
           S  SG + +VNVD F A G+G  DD++AF  AW  ACS+  + LLVP+GR+Y +    F 
Sbjct: 59  SQYSG-KNLVNVDSFNASGDGVSDDTQAFIRAWTMACSAPNSVLLVPQGRSYLVNATKFD 118

Query: 130 GPCRAPLTLMINGMIKASPIMSDYD-NDRRHWLKFEGVDNFVVEGNGIINGNGRKWWQNS 189
           GPC+  L + I+G I A    S +D    R+WL+F  +   V +GNG+I+G+G KWW  S
Sbjct: 119 GPCQEKLIIQIDGTIIAPDEPSQWDPKFPRNWLQFSKLQGVVFQGNGVIDGSGTKWWAAS 178

Query: 190 CKVNKE-------LAVTFYQCINLVVANLMFKNAQQMHLTFQKCTNVKALNLRVVAPGNS 249
           CK NK         A+T Y   N+ V  L  +N+QQMHL  Q+ T V+   + V +PG+S
Sbjct: 179 CKKNKSNPCVGAPTALTIYSSSNVYVRGLTIRNSQQMHLIIQRSTTVRISRVMVTSPGDS 238

Query: 250 PNTDGIHITETQNAIIRNCVIGTGDDCISIVSGSRNVQAMDITCGPGHGISIGSLGAENS 309
           PNTDGIHIT + + ++++  I TGDDC+SIV+GS  ++   I CGPGHGISIGSLG  +S
Sbjct: 239 PNTDGIHITASTDVVVQDSKISTGDDCVSIVNGSAKIKMKRIYCGPGHGISIGSLGQGHS 298

Query: 310 EAEVSNIRVNRALISGTSNGVRIKTWQGGSGYAKNIMFQNVVMNNVSNPIIIDQNYCDKK 369
           +  V+ + +  A +  T+NG+RIKTWQGG+GY K + F+NVVM +V+NPIIIDQ YCD  
Sbjct: 299 KGTVTAVVLETAFLKNTTNGLRIKTWQGGNGYVKGVRFENVVMQDVANPIIIDQFYCDSP 358

Query: 370 DSCPEQTSAVKVSNVVYKNIRGTSASDDAIKFDCSKSFPCQEISLLGVHIVGQGDEV 419
            +C  QTSAV +S ++Y+NI GT+ S  AI F CS + PC  I L  +++ G   +V
Sbjct: 359 STCQNQTSAVHISEIMYRNITGTTKSSKAINFKCSDAVPCSHIVLNNINLEGNDGKV 414