HG10017905 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10017905
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionSerine proteinase inhibitor
LocationChr03: 25770709 .. 25770930 (-)
RNA-Seq ExpressionHG10017905
SyntenyHG10017905
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATATTATTTTGATCCATTAGGAAAATCATCATGGCCAGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCTTGTCATACAGAAAGACAATCCTAGAGTGGAAATAATTGACATTATATTAGCCGGTAGTCCAGTGCCTCGTGACTTTAGACAGGATCGAGTTCGAATTTTTGTGAACATACGAAATGTTGCAGTTGAGATACCAATCATTGGTTAA

mRNA sequence

ATGAAATATTATTTTGATCCATTAGGAAAATCATCATGGCCAGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCTTGTCATACAGAAAGACAATCCTAGAGTGGAAATAATTGACATTATATTAGCCGGTAGTCCAGTGCCTCGTGACTTTAGACAGGATCGAGTTCGAATTTTTGTGAACATACGAAATGTTGCAGTTGAGATACCAATCATTGGTTAA

Coding sequence (CDS)

ATGAAATATTATTTTGATCCATTAGGAAAATCATCATGGCCAGAACTTGTGGGAATTGAAGCTGAAATTGCAAAGCTTGTCATACAGAAAGACAATCCTAGAGTGGAAATAATTGACATTATATTAGCCGGTAGTCCAGTGCCTCGTGACTTTAGACAGGATCGAGTTCGAATTTTTGTGAACATACGAAATGTTGCAGTTGAGATACCAATCATTGGTTAA

Protein sequence

MKYYFDPLGKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEIPIIG
Homology
BLAST of HG10017905 vs. NCBI nr
Match: KAG6571499.1 (Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 87.0 bits (214), Expect = 7.1e-14
Identity = 42/69 (60.87%), Postives = 50/69 (72.46%), Query Frame = 0

Query: 5  FDPLGKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRN 64
          F P GK+ WPELVGIEA I + +I+K+NP V I+ I LAGSPVP DF   RVR+FVNI +
Sbjct: 4  FCPPGKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNIND 63

Query: 65 VAVEIPIIG 74
          V V IP  G
Sbjct: 64 VVVSIPWTG 72

BLAST of HG10017905 vs. NCBI nr
Match: KAG6571494.1 (hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 85.1 bits (209), Expect = 2.7e-13
Identity = 41/69 (59.42%), Postives = 52/69 (75.36%), Query Frame = 0

Query: 5  FDPLGKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRN 64
          F P GK+ WPELVGI++ IA+ +I+KDN  VE ++ ILAGS V  D R DRVRIFVNI+ 
Sbjct: 4  FCPPGKNRWPELVGIKSTIAEHIIRKDNSNVEHVETILAGSAVTEDLRCDRVRIFVNIKG 63

Query: 65 VAVEIPIIG 74
          + V+IP IG
Sbjct: 64 IIVQIPTIG 72

BLAST of HG10017905 vs. NCBI nr
Match: XP_004247658.1 (wound-induced proteinase inhibitor 1 [Solanum lycopersicum])

HSP 1 Score: 83.2 bits (204), Expect = 1.0e-12
Identity = 40/65 (61.54%), Postives = 49/65 (75.38%), Query Frame = 0

Query: 9   GKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVE 68
           GK SWPEL+G+ A+ AK +IQK+NP V  + I+L GSPV  DFR  RVRI VNI NVAV 
Sbjct: 47  GKLSWPELIGVPAQYAKGIIQKENPFVTDVQIVLNGSPVTADFRCFRVRIVVNILNVAVS 106

Query: 69  IPIIG 74
           IP++G
Sbjct: 107 IPVVG 111

BLAST of HG10017905 vs. NCBI nr
Match: TMW88525.1 (hypothetical protein EJD97_018437 [Solanum chilense])

HSP 1 Score: 80.9 bits (198), Expect = 5.1e-12
Identity = 38/65 (58.46%), Postives = 49/65 (75.38%), Query Frame = 0

Query: 9   GKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVE 68
           GK SWPEL+G+ A+ AK +IQK+NP +  + I+L GSPV  DFR  RVRI VNI +VAV 
Sbjct: 47  GKLSWPELIGVPAQYAKGIIQKENPFITDVQIVLNGSPVTADFRCFRVRIVVNILDVAVS 106

Query: 69  IPIIG 74
           IP++G
Sbjct: 107 IPVVG 111

BLAST of HG10017905 vs. NCBI nr
Match: KGN50840.1 (hypothetical protein Csa_004718 [Cucumis sativus])

HSP 1 Score: 80.5 bits (197), Expect = 6.7e-12
Identity = 36/67 (53.73%), Postives = 49/67 (73.13%), Query Frame = 0

Query: 7  PLGKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVA 66
          P GK+ WPELVG++A  AK +I+KDNP VE + ++LAGS    D R DRV +FVNI ++ 
Sbjct: 6  PPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHDLV 65

Query: 67 VEIPIIG 74
          VE+P +G
Sbjct: 66 VEVPKVG 72

BLAST of HG10017905 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.7e-13
Identity = 35/62 (56.45%), Postives = 45/62 (72.58%), Query Frame = 0

Query: 9   GKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVE 68
           GK  WPEL+G+ A  AK +I+K+NP +  I I+L GSPV +DFR DRVR+FVNI    V+
Sbjct: 47  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 69  IP 71
           IP
Sbjct: 107 IP 108

BLAST of HG10017905 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.4e-12
Identity = 30/64 (46.88%), Postives = 48/64 (75.00%), Query Frame = 0

Query: 10 KSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEI 69
          K +WPEL+G+ A+ A+ +IQK+N ++  +  +L GSPV +DFR +RVR+FVN+ +  V+I
Sbjct: 31 KETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQI 90

Query: 70 PIIG 74
          P +G
Sbjct: 91 PRVG 94

BLAST of HG10017905 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 4.1e-12
Identity = 32/69 (46.38%), Postives = 46/69 (66.67%), Query Frame = 0

Query: 5   FDPLGKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRN 64
           F   GK  WPEL+G+  ++AK +I+K+N  +  + I+L GSPV  D R DRVR+F NI  
Sbjct: 39  FQCKGKLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILG 98

Query: 65  VAVEIPIIG 74
             V+IP++G
Sbjct: 99  YVVDIPVVG 107

BLAST of HG10017905 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 6.9e-12
Identity = 29/64 (45.31%), Postives = 46/64 (71.88%), Query Frame = 0

Query: 9   GKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVE 68
           GK  WPEL+G+  ++AK +I+K+NP +  I I+L+GSP+  D+  DRVR+F NI    V+
Sbjct: 47  GKQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQ 106

Query: 69  IPII 73
           +P++
Sbjct: 107 MPVV 110

BLAST of HG10017905 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 2.6e-11
Identity = 33/65 (50.77%), Postives = 46/65 (70.77%), Query Frame = 0

Query: 9  GKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVE 68
          GKSSWP LVG+   +AK +I++ NP V+ + I+  G+PV +DFR +RVRI+VN R + V 
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAV-ILEEGTPVTKDFRCNRVRIWVNKRGLVVS 64

Query: 69 IPIIG 74
           P IG
Sbjct: 65 PPRIG 68

BLAST of HG10017905 vs. ExPASy TrEMBL
Match: A0A6N2B0Y4 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_018437 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.5e-12
Identity = 38/65 (58.46%), Postives = 49/65 (75.38%), Query Frame = 0

Query: 9   GKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVE 68
           GK SWPEL+G+ A+ AK +IQK+NP +  + I+L GSPV  DFR  RVRI VNI +VAV 
Sbjct: 47  GKLSWPELIGVPAQYAKGIIQKENPFITDVQIVLNGSPVTADFRCFRVRIVVNILDVAVS 106

Query: 69  IPIIG 74
           IP++G
Sbjct: 107 IPVVG 111

BLAST of HG10017905 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.2e-12
Identity = 36/67 (53.73%), Postives = 49/67 (73.13%), Query Frame = 0

Query: 7  PLGKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVA 66
          P GK+ WPELVG++A  AK +I+KDNP VE + ++LAGS    D R DRV +FVNI ++ 
Sbjct: 6  PPGKNQWPELVGVKATTAKYIIKKDNPNVENVVVLLAGSGTTEDIRCDRVWVFVNIHDLV 65

Query: 67 VEIPIIG 74
          VE+P +G
Sbjct: 66 VEVPKVG 72

BLAST of HG10017905 vs. ExPASy TrEMBL
Match: A0A6N2AU12 (Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023458 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.1e-11
Identity = 36/62 (58.06%), Postives = 46/62 (74.19%), Query Frame = 0

Query: 9   GKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVE 68
           GK  WPEL+G+ A  AK +I+K+NP +  I I+L GSPV +DFR DRVR+FVNI  V V+
Sbjct: 40  GKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGVVVQ 99

Query: 69  IP 71
           IP
Sbjct: 100 IP 101

BLAST of HG10017905 vs. ExPASy TrEMBL
Match: Q40444 (Tumor-related protein (Fragment) OS=Nicotiana glauca x Nicotiana langsdorffii OX=79230 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.6e-11
Identity = 32/64 (50.00%), Postives = 50/64 (78.12%), Query Frame = 0

Query: 10 KSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEI 69
          K +WPEL+G+ A+ A+ +IQK+NP++  +  +L GSPV +DFR +RVR+FVN+ +V V+I
Sbjct: 1  KETWPELIGVPAKFAREIIQKENPKLTNVPSVLNGSPVTKDFRCNRVRLFVNVLDVVVQI 60

Query: 70 PIIG 74
          P +G
Sbjct: 61 PRVG 64

BLAST of HG10017905 vs. ExPASy TrEMBL
Match: A0A3Q7I868 (Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101247557 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.6e-11
Identity = 35/62 (56.45%), Postives = 46/62 (74.19%), Query Frame = 0

Query: 9   GKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVE 68
           GK  WPEL+G+ A+ AK +I+K+NP +  I I+L GSPV +DFR DRVR+FVNI    V+
Sbjct: 47  GKQFWPELIGVPAQYAKGIIEKENPSIANIPILLNGSPVTKDFRCDRVRLFVNILGDVVQ 106

Query: 69  IP 71
           IP
Sbjct: 107 IP 108

BLAST of HG10017905 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 65.5 bits (158), Expect = 2.1e-11
Identity = 35/68 (51.47%), Postives = 49/68 (72.06%), Query Frame = 0

Query: 6  DPLGKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNV 65
          DP GKSSWPEL+G + E AK VI+++NP+++ + IIL G+ VP  F   RV ++VN   +
Sbjct: 34 DP-GKSSWPELLGAKGEDAKEVIERENPKMKAV-IILDGTVVPEIFICSRVYVWVNDCGI 93

Query: 66 AVEIPIIG 74
           V+IPIIG
Sbjct: 94 VVQIPIIG 99

BLAST of HG10017905 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 63.2 bits (152), Expect = 1.0e-10
Identity = 31/64 (48.44%), Postives = 42/64 (65.62%), Query Frame = 0

Query: 10 KSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEI 69
          K+SWPEL G   + A +VI+++NP V    +IL GSPV  DFR DRVR+FV+   + V+ 
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAA-VILDGSPVTADFRCDRVRVFVDGNRIVVKT 67

Query: 70 PIIG 74
          P  G
Sbjct: 68 PKSG 70

BLAST of HG10017905 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 46.2 bits (108), Expect = 1.3e-05
Identity = 24/75 (32.00%), Postives = 42/75 (56.00%), Query Frame = 0

Query: 1  MKYYFD--PLGKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRI 60
          + +YF    +GK+SWPEL+G   + A  VI+ +N  + ++ ++  G+ V  D    RVR+
Sbjct: 15 LNFYFSFFDIGKNSWPELLGTNGDYAASVIKGENSSLNVV-VVSDGNYVTEDLSCYRVRV 74

Query: 61 FVNIRNVAVEIPIIG 74
          +V+   + V  P  G
Sbjct: 75 WVDEIRIVVRNPTAG 88

BLAST of HG10017905 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 44.3 bits (103), Expect = 4.9e-05
Identity = 22/66 (33.33%), Postives = 38/66 (57.58%), Query Frame = 0

Query: 8  LGKSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAV 67
          +GK+SWPEL+G   + A  VI+ +N  + ++ ++  G+ V  D    RVR++V+   + V
Sbjct: 9  IGKNSWPELLGTNGDYAASVIKGENSSLNVV-VVSDGNYVTEDLSCYRVRVWVDEIRIVV 68

Query: 68 EIPIIG 74
            P  G
Sbjct: 69 RNPTAG 73

BLAST of HG10017905 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 42.4 bits (98), Expect = 1.9e-04
Identity = 21/64 (32.81%), Postives = 36/64 (56.25%), Query Frame = 0

Query: 10 KSSWPELVGIEAEIAKLVIQKDNPRVEIIDIILAGSPVPRDFRQDRVRIFVNIRNVAVEI 69
          K  WPELVG   ++AK+ I+++NP V  I ++  G     +F  +RV +++       + 
Sbjct: 23 KYRWPELVGKNGQLAKMTIERENPNVLAI-VLRYGEKRIENFCCNRVFVYLGSNGQVADA 82

Query: 70 PIIG 74
          P+IG
Sbjct: 83 PMIG 85

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6571499.17.1e-1460.87Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6571494.12.7e-1359.42hypothetical protein SDJN03_28222, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_004247658.11.0e-1261.54wound-induced proteinase inhibitor 1 [Solanum lycopersicum][more]
TMW88525.15.1e-1258.46hypothetical protein EJD97_018437 [Solanum chilense][more]
KGN50840.16.7e-1253.73hypothetical protein Csa_004718 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
P162313.7e-1356.45Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1[more]
Q022142.4e-1246.88Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1[more]
Q007834.1e-1246.38Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1[more]
P051186.9e-1245.31Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=... [more]
P198732.6e-1150.77Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6N2B0Y42.5e-1258.46Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_018437 PE=3 SV=1[more]
A0A0A0KME73.2e-1253.73Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1[more]
A0A6N2AU122.1e-1158.06Uncharacterized protein OS=Solanum chilense OX=4083 GN=EJD97_023458 PE=3 SV=1[more]
Q404443.6e-1150.00Tumor-related protein (Fragment) OS=Nicotiana glauca x Nicotiana langsdorffii OX... [more]
A0A3Q7I8683.6e-1156.45Uncharacterized protein OS=Solanum lycopersicum OX=4081 GN=101247557 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G43580.12.1e-1151.47Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38870.11.0e-1048.44Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.21.3e-0532.00Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT2G38900.14.9e-0533.33Serine protease inhibitor, potato inhibitor I-type family protein [more]
AT3G46860.11.9e-0432.81Serine protease inhibitor, potato inhibitor I-type family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000864Proteinase inhibitor I13, potato inhibitor IPRINTSPR00292POTATOINHBTRcoord: 12..25
score: 57.14
coord: 50..60
score: 59.29
coord: 38..49
score: 38.04
IPR000864Proteinase inhibitor I13, potato inhibitor IPFAMPF00280potato_inhibitcoord: 10..73
e-value: 3.3E-23
score: 82.1
IPR000864Proteinase inhibitor I13, potato inhibitor IPANTHERPTHR33091PROTEIN, PUTATIVE, EXPRESSED-RELATEDcoord: 8..73
IPR000864Proteinase inhibitor I13, potato inhibitor IPROSITEPS00285POTATO_INHIBITORcoord: 13..24
NoneNo IPR availableGENE3D3.30.10.10Trypsin Inhibitor V, subunit Acoord: 6..73
e-value: 9.7E-23
score: 82.2
NoneNo IPR availablePANTHERPTHR33091:SF83ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1coord: 8..73
IPR036354Proteinase inhibitor I13, potato inhibitor I superfamilySUPERFAMILY54654CI-2 family of serine protease inhibitorscoord: 8..73

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10017905.1HG10017905.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010466 negative regulation of peptidase activity
biological_process GO:0009611 response to wounding
molecular_function GO:0004867 serine-type endopeptidase inhibitor activity