HG10014553 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10014553 vs. NCBI nr
Match: XP_038893069.1 (subtilisin-like protease SBT1.1 [Benincasa hispida] >XP_038893070.1 subtilisin-like protease SBT1.1 [Benincasa hispida])

HSP 1 Score: 1341.6 bits (3471), Expect = 0.0e+00
Identity = 684/767 (89.18%), Postives = 710/767 (92.57%), Query Frame = 0

Query: 1   MGFREVWLLLSIMLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSL-DH 60
           MGFREVW+ LSIMLAI++AAVDQQTYIIHMDTTKM T NPEQWYT +IDS+NEL SL D 
Sbjct: 1   MGFREVWMFLSIMLAIATAAVDQQTYIIHMDTTKMTTTNPEQWYTAMIDSLNELPSLDDE 60

Query: 61  NDEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFL 120
           N EEAS+ AEILY            LS KKL+SLSKI GFLAATPNELLQLHTTHSPQFL
Sbjct: 61  NKEEASDTAEILYVYKTALSGFAAKLSRKKLDSLSKIPGFLAATPNELLQLHTTHSPQFL 120

Query: 121 GLQKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSN 180
           GL++DHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGL PVPSKWKGICQAG KFSPSN
Sbjct: 121 GLERDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPSKWKGICQAGPKFSPSN 180

Query: 181 CNKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAM 240
           CNKKLIGASAYIKGYEAIVG LNETGTFRSPRDSDGHGTHTASTAAG++VNKAS  NQ M
Sbjct: 181 CNKKLIGASAYIKGYEAIVGTLNETGTFRSPRDSDGHGTHTASTAAGSMVNKASFFNQGM 240

Query: 241 GVATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIA 300
           GVATGM +TSRIAAYKVCWPLGCA+ADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIA
Sbjct: 241 GVATGMMYTSRIAAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIA 300

Query: 301 IAAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEG 360
           IAAFGAIQNGVFVSCSAGNSGP  STVGNAAPWIMTVAASYTDR FPTTVKLGNGQVFEG
Sbjct: 301 IAAFGAIQNGVFVSCSAGNSGPFISTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEG 360

Query: 361 SSLYVGKNINELPLVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQV 420
           SSLY GKNIN LPLVYNNTAGDGQETNFCT GSLDP+ V GKIVLCERG NSRT KGEQV
Sbjct: 361 SSLYYGKNINYLPLVYNNTAGDGQETNFCTAGSLDPTMVKGKIVLCERGSNSRTDKGEQV 420

Query: 421 KLAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASIAFEGT 480
           KLAGGAGMILIN QFEGEELFADPHVLPATTLGASAG  ILDYIASS+SQAKAS+AFEGT
Sbjct: 421 KLAGGAGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKSQAKASMAFEGT 480

Query: 481 KYGSRAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNI 540
           KYGSRAPRVAAFSSRGPSFVGPDV+KPDVTAPGVNILAAWPPIVSPSEL+SDKRRVLFNI
Sbjct: 481 KYGSRAPRVAAFSSRGPSFVGPDVMKPDVTAPGVNILAAWPPIVSPSELDSDKRRVLFNI 540

Query: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPAD 600
           ISGTSMSCPHVSGLAALLKSAHKDWSPAA+KSALMTTAYVTDNKM+L+SDVG  SGGPAD
Sbjct: 541 ISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKMNLVSDVGHPSGGPAD 600

Query: 601 PFAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQ 660
           PFAFGSGHV PEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRG  TCSSKRT LQ
Sbjct: 601 PFAFGSGHVDPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTLLQ 660

Query: 661 PGDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFG 720
           P DLNYPSFSVFMKKKAKNVSIT KRTVTNVGIPRSDYTVKINNPKGI + VKPEKLSFG
Sbjct: 661 PRDLNYPSFSVFMKKKAKNVSITSKRTVTNVGIPRSDYTVKINNPKGIRITVKPEKLSFG 720

Query: 721 SLGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
           SLGEKLS+QV FVALGGK+ L +FSFG LVW+SGKYAVRSPIAVTWQ
Sbjct: 721 SLGEKLSFQVSFVALGGKEALSKFSFGDLVWLSGKYAVRSPIAVTWQ 767

BLAST of HG10014553 vs. NCBI nr
Match: XP_008454762.1 (PREDICTED: subtilisin-like protease SBT1.1 [Cucumis melo] >XP_016901624.1 PREDICTED: subtilisin-like protease SBT1.1 [Cucumis melo])

HSP 1 Score: 1298.5 bits (3359), Expect = 0.0e+00
Identity = 665/766 (86.81%), Postives = 698/766 (91.12%), Query Frame = 0

Query: 1   MGFREVWLLLSIMLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSLDHN 60
           MGFREVW +LSIMLAISSA VDQQTYIIHMDTTKM+T NPEQWYT IIDSVNELSSLD N
Sbjct: 1   MGFREVW-VLSIMLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDDN 60

Query: 61  DEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLG 120
            EEASNAAEILY            L++KKL+SLSKI GFLAATPNELLQLHTTHSPQFLG
Sbjct: 61  -EEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120

Query: 121 LQKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNC 180
           LQ+DHGLWN SNLASDIIIGLLDTGIWPEHISFQDKGL  VP KWKGICQ G +FS SNC
Sbjct: 121 LQRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNC 180

Query: 181 NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMG 240
           NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAG+IV+ AS  NQ MG
Sbjct: 181 NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMG 240

Query: 241 VATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAI 300
           VA+GM FTSRI AYKVCWPLGCA+ADILAAMDSAVADGVDVLSLSLGGG+ SFY+DNIAI
Sbjct: 241 VASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 300

Query: 301 AAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGS 360
           AAFGAIQ GVFVSCSAGNSGPS STVGNAAPWIMTVAASYTDR FPTTVKLGNGQVFEGS
Sbjct: 301 AAFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 360

Query: 361 SLYVGKNINELPLVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVK 420
           SLY GK+INELPLVYNNTAGDGQETN C  GSLDPS V GKIV+CERG  SRT KGEQVK
Sbjct: 361 SLYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVK 420

Query: 421 LAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTK 480
           LAGG GMILIN QFEGEELFADPHVLPATTLGASAG  ILDYIASS++QAKASI FEGTK
Sbjct: 421 LAGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTK 480

Query: 481 YGSRAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNII 540
           YGS+APRVAAFSSRGPS VGPDVIKPDVTAPGVNILAAWPPIVSPSEL SD RRV+FNII
Sbjct: 481 YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNII 540

Query: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADP 600
           SGTSMSCPHVSGLAALLKSAH DWSPAA+KSALMTTAYVTD+KMSLISDVG A+G PA P
Sbjct: 541 SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATP 600

Query: 601 FAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQP 660
           F FGSGHV PEKASDPGL+YDITPQDYINYLCSLKYNS+QIALVSRG LTCSSKRT ++P
Sbjct: 601 FTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKP 660

Query: 661 GDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGS 720
           GDLNYPSFSVFMKKKAK VSIT KRTVTNVGI RSDYTVKINNPKG+TVIVKPEKLSFGS
Sbjct: 661 GDLNYPSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGS 720

Query: 721 LGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
           LGE+LSY+V FV+LGGK+ L +FSFGSLVW+SGKYAVRSPI VTWQ
Sbjct: 721 LGEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of HG10014553 vs. NCBI nr
Match: XP_004140440.1 (subtilisin-like protease SBT1.1 [Cucumis sativus] >KGN50768.2 hypothetical protein Csa_021519 [Cucumis sativus])

HSP 1 Score: 1294.6 bits (3349), Expect = 0.0e+00
Identity = 658/766 (85.90%), Postives = 695/766 (90.73%), Query Frame = 0

Query: 1   MGFREVWLLLSIMLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSLDHN 60
           MGFREVW+LLSIMLA+SSA VDQQTYIIHMD TKM+T  PEQWYT IIDSVN+LSSLD N
Sbjct: 1   MGFREVWVLLSIMLAVSSAVVDQQTYIIHMDATKMVTPIPEQWYTDIIDSVNKLSSLDDN 60

Query: 61  DEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLG 120
           +EEASNAAEILY            L++KKL+SLSKI GFLAATPNELLQLHTTHSPQFLG
Sbjct: 61  EEEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120

Query: 121 LQKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNC 180
           LQ+DHGLWNSSNLASDIIIGLLDTG+WPEHISFQD+ L  VP KWKGICQ G +FS SNC
Sbjct: 121 LQRDHGLWNSSNLASDIIIGLLDTGVWPEHISFQDESLSSVPLKWKGICQTGPRFSSSNC 180

Query: 181 NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMG 240
           NKKLIGAS YIKGYEAIVGRLNETG FRSPRDS+GHGTHTASTAAG+IVN AS  NQ MG
Sbjct: 181 NKKLIGASFYIKGYEAIVGRLNETGIFRSPRDSNGHGTHTASTAAGSIVNNASFFNQGMG 240

Query: 241 VATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAI 300
           VA+G+ FTSRI AYKVCWPLGCA+ADILAAMDSAVADGVDVLSLSLGGG+ SFY+DNIAI
Sbjct: 241 VASGIRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 300

Query: 301 AAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGS 360
           AAFGAI+ GVFVSCSAGNSGPS STVGNAAPWIMTVAASYTDR FPTTVKLGNGQVFEGS
Sbjct: 301 AAFGAIEKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 360

Query: 361 SLYVGKNINELPLVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVK 420
           SLY GK+INELPLVYNNTAGDGQETNFC  GSLDPS V GKIV+CERG  SRT KGEQVK
Sbjct: 361 SLYYGKSINELPLVYNNTAGDGQETNFCIAGSLDPSMVKGKIVVCERGQISRTEKGEQVK 420

Query: 421 LAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTK 480
           LAGGAGMILIN +FEGEELFADPH+LPATTLGA AG  ILDY ASS++QAKA I FEGTK
Sbjct: 421 LAGGAGMILINTEFEGEELFADPHILPATTLGALAGKAILDYTASSKTQAKALIVFEGTK 480

Query: 481 YGSRAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNII 540
           YGS+APRVAAFSSRGPS VGPDVIKPDVTAPGVNILAAWPPIVSPSELESD RRVLFNII
Sbjct: 481 YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDTRRVLFNII 540

Query: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADP 600
           SGTSMSCPHVSGLAALLKSAH DWSPAA+KSALMTTAY+TDNKMSLISDVG A+G PA P
Sbjct: 541 SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYITDNKMSLISDVGQANGEPATP 600

Query: 601 FAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQP 660
           F FGSGHV PEKASDPGL+YDITPQDYINYLCSLKYNSTQIALVSRG  TCSSKRT ++P
Sbjct: 601 FTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSTQIALVSRGNFTCSSKRTVVKP 660

Query: 661 GDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGS 720
           GDLNYPSFSVFMKKKAK VSIT KRTVTNVGI RSDYTVKINNPKGITVIVKPEKLSFGS
Sbjct: 661 GDLNYPSFSVFMKKKAKKVSITLKRTVTNVGISRSDYTVKINNPKGITVIVKPEKLSFGS 720

Query: 721 LGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
           LGE+LSYQV FV+LGGK+ L  FSFGSLVW+SGKYAVRSPIAVTWQ
Sbjct: 721 LGEQLSYQVRFVSLGGKEALDTFSFGSLVWISGKYAVRSPIAVTWQ 766

BLAST of HG10014553 vs. NCBI nr
Match: KAA0056497.1 (subtilisin-like protease SBT1.1 [Cucumis melo var. makuwa] >TYK29026.1 subtilisin-like protease SBT1.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1282.7 bits (3318), Expect = 0.0e+00
Identity = 655/754 (86.87%), Postives = 687/754 (91.11%), Query Frame = 0

Query: 13  MLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSLDHNDEEASNAAEILY 72
           MLAISSA VDQQTYIIHMDTTKM+T NPEQWYT IIDSVNELSSLD N EEASNAAEILY
Sbjct: 1   MLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDDN-EEASNAAEILY 60

Query: 73  ------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLGLQKDHGLWNSSN 132
                       L++KKL+SLSKI GFLAATPNELLQLHTTHSPQFLGLQ+DHGLWN SN
Sbjct: 61  VYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHGLWNFSN 120

Query: 133 LASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNCNKKLIGASAYIK 192
           LASDIIIGLLDTGIWPEHISFQDKGL  VP KWKGICQ G +FS SNCNKKLIGASAYIK
Sbjct: 121 LASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNCNKKLIGASAYIK 180

Query: 193 GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMGVATGMSFTSRIA 252
           GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAG+IV+ AS  NQ MGVA+GM FTSRI 
Sbjct: 181 GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMGVASGMRFTSRIV 240

Query: 253 AYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGVFV 312
           AYKVCWPLGCA+ADILAAMDSAVADGVDVLSLSLGGG+ SFY+DNIAIAAFGAIQ GVFV
Sbjct: 241 AYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIQKGVFV 300

Query: 313 SCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGSSLYVGKNINELP 372
           SCSAGNSGPS STVGNAAPWIMTVAASYTDR FPTTVKLGNGQVFEGSSLY GK+INELP
Sbjct: 301 SCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSINELP 360

Query: 373 LVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVKLAGGAGMILINA 432
           LVYNNTAGDGQETN C  GSLDPS V GKIV+CERG  SRT KGEQVKLAGG GMILIN 
Sbjct: 361 LVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVKLAGGTGMILINT 420

Query: 433 QFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTKYGSRAPRVAAFS 492
           QFEGEELFADPHVLPATTLGASAG  ILDYIASS++QAKASI FEGTKYGS+APRVAAFS
Sbjct: 421 QFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTKYGSQAPRVAAFS 480

Query: 493 SRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSG 552
           SRGPS VGPDVIKPDVTAPGVNILAAWPPIVSPSEL SD RRV+FNIISGTSMSCPHVSG
Sbjct: 481 SRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNIISGTSMSCPHVSG 540

Query: 553 LAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADPFAFGSGHVHPEK 612
           LAALLKSAH DWSPAA+KSALMTTAYVTD+KMSLISDVG A+G PA PF FGSGHV PEK
Sbjct: 541 LAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATPFTFGSGHVDPEK 600

Query: 613 ASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQPGDLNYPSFSVFM 672
           ASDPGL+YDITPQDYINYLCSLKYNS+QIALVSRG LTCSSKRT ++PGDLNYPSFSVFM
Sbjct: 601 ASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKPGDLNYPSFSVFM 660

Query: 673 KKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGSLGEKLSYQVGFV 732
           KKKAK VSIT KRTVTNVGI RSDYTVKINNPKG+TVIVKPEKLSFGSLGE+LSY+V FV
Sbjct: 661 KKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGSLGEQLSYKVSFV 720

Query: 733 ALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
           +LGGK+ L +FSFGSLVW+SGKYAVRSPI VTWQ
Sbjct: 721 SLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 753

BLAST of HG10014553 vs. NCBI nr
Match: XP_038891850.1 (subtilisin-like protease SBT1.1 [Benincasa hispida] >XP_038891851.1 subtilisin-like protease SBT1.1 [Benincasa hispida] >XP_038891852.1 subtilisin-like protease SBT1.1 [Benincasa hispida])

HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 641/772 (83.03%), Postives = 685/772 (88.73%), Query Frame = 0

Query: 1   MGFREVWLLLSIMLAI---SSAAVDQQTYIIHMDTTKMIT-NNPEQWYTTIIDSVNELSS 60
           M  R++ + LSI +AI   SSAAVDQQ+YIIHMDT+KM T NNPEQWYT +IDSVNEL+S
Sbjct: 1   MKIRQMLMFLSITIAILATSSAAVDQQSYIIHMDTSKMATANNPEQWYTVMIDSVNELAS 60

Query: 61  L--DHNDEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTH 120
           L  D+N+EEAS AAEILY            LSTK L+SLSKI GFLAATPN+LLQLHTTH
Sbjct: 61  LDNDNNEEEASTAAEILYVYKTAISGFAAKLSTKHLHSLSKIPGFLAATPNKLLQLHTTH 120

Query: 121 SPQFLGLQKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAK 180
           SPQFLGL++  GLWNSSNLASDIIIGLLDTGIWPEHISFQDKGL PVP+KWKGICQAG K
Sbjct: 121 SPQFLGLKRGRGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLSPVPTKWKGICQAGPK 180

Query: 181 FSPSNCNKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASL 240
           FSPSNCNKKLIGA A+I+GYEA+VGRLNETGTFRSPRDSDGHGTHTASTAAGN VN+AS 
Sbjct: 181 FSPSNCNKKLIGARAFIQGYEAVVGRLNETGTFRSPRDSDGHGTHTASTAAGNFVNRASF 240

Query: 241 NNQAMGVATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFY 300
            NQA+G ATGM FTSRIAAYKVCWP GCASADILAAMD A+ADGVDVLS+SLGGG+G FY
Sbjct: 241 YNQALGAATGMRFTSRIAAYKVCWPEGCASADILAAMDHAIADGVDVLSISLGGGSGIFY 300

Query: 301 RDNIAIAAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNG 360
            D IAIAAFGAIQ GVFVSCSAGNSGP  STVGN APWIMTVAASYTDR FPTTVKLGNG
Sbjct: 301 SDEIAIAAFGAIQQGVFVSCSAGNSGPYISTVGNVAPWIMTVAASYTDRTFPTTVKLGNG 360

Query: 361 QVFEGSSLYVGKNINELPLVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTA 420
           +VFEGSSLY GKNINE+PLVYNNTAGDG+E+N CT GSL PS V GKIV+CERG NSRTA
Sbjct: 361 KVFEGSSLYFGKNINEIPLVYNNTAGDGEESNVCTAGSLVPSMVKGKIVVCERGTNSRTA 420

Query: 421 KGEQVKLAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASI 480
           KGEQVKLAGGAGMILIN Q EGEEL AD HVLPAT +GASA   I+DYIASS+ QAKASI
Sbjct: 421 KGEQVKLAGGAGMILINTQLEGEELIADSHVLPATAVGASASKAIIDYIASSKHQAKASI 480

Query: 481 AFEGTKYGSRAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRR 540
            F+GTKYGS+APRVAAFSSRGPSF  P VIKPD+TAPGVNILAAWPPIVSPSELESDKRR
Sbjct: 481 TFKGTKYGSQAPRVAAFSSRGPSFFKPYVIKPDITAPGVNILAAWPPIVSPSELESDKRR 540

Query: 541 VLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDAS 600
           VLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAA+KSALMTTAYVTDNK  LISDV  AS
Sbjct: 541 VLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYVTDNKNHLISDVSRAS 600

Query: 601 GGPADPFAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSK 660
           GGPADP+AFGSGHV PEKAS+PGLVYDI PQDYINYLCSLKYNS QIALVSRGK TCSSK
Sbjct: 601 GGPADPYAFGSGHVDPEKASNPGLVYDIAPQDYINYLCSLKYNSAQIALVSRGKFTCSSK 660

Query: 661 RTFLQPGDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPE 720
           R FLQPGDLNYPSFS+FMKKKAKNV+ITFKRTVTNVGIPRSDYT KINNP+GI VIVKPE
Sbjct: 661 RKFLQPGDLNYPSFSLFMKKKAKNVTITFKRTVTNVGIPRSDYTAKINNPEGIRVIVKPE 720

Query: 721 KLSFGSLGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
           KLSF  LG KLSY+V FVALG ++ L +FSFGSLVW SGKYAVRSPIAVTWQ
Sbjct: 721 KLSFVRLGGKLSYKVSFVALGKRETLDDFSFGSLVWQSGKYAVRSPIAVTWQ 772

BLAST of HG10014553 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 4.2e-229
Identity = 418/767 (54.50%), Postives = 533/767 (69.49%), Query Frame = 0

Query: 1   MGFREVWLLLSIMLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSLDHN 60
           M FR   +   ++   S+ +  +QTY+IH      +T + +   T++    N L + + N
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLF---NSLQTENIN 78

Query: 61  DEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLG 120
           D++ S   EI Y            L+  +L+++   +GF++A P+ELL LHTT+S +FLG
Sbjct: 79  DDDFS-LPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLG 138

Query: 121 LQKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNC 180
           L+   GLWN ++L+SD+IIGL+DTGI PEH+SF+D  + PVPS+W+G C  G  FS S C
Sbjct: 139 LEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSEC 198

Query: 181 NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMG 240
           NKK+IGASA+ KGYE+IVG++NET  FRS RD+ GHGTHTASTAAG+IV KA+   QA G
Sbjct: 199 NKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKG 258

Query: 241 VATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAI 300
           +A+GM FTSRIAAYK CW LGCAS D++AA+D A+ DGVDV+SLSLGG +  FY D IAI
Sbjct: 259 LASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAI 318

Query: 301 AAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGS 360
           A FGA+Q  +FVSCSAGNSGP+ STV N APW+MTVAASYTDR FP  V++GN +   GS
Sbjct: 319 AGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGS 378

Query: 361 SLYVGKNINELPLVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVK 420
           SLY GK++  LPL +N TAG+     FC   SL    V GKIV+C RG + RTAKGE+VK
Sbjct: 379 SLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVK 438

Query: 421 LAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTK 480
            +GGA M+L++ + EGEEL ADPHVLPA +LG S G  +L+Y+A + + A AS+ F GT 
Sbjct: 439 RSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGA-ANATASVRFRGTA 498

Query: 481 YGSRAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNII 540
           YG+ AP VAAFSSRGPS  GP++ KPD+ APG+NILA W P  SPS L SD RRV FNII
Sbjct: 499 YGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNII 558

Query: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASG-GPAD 600
           SGTSM+CPH+SG+AAL+KS H DWSPA +KSA+MTTA +TDN+   I D G A     A 
Sbjct: 559 SGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAAT 618

Query: 601 PFAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQ 660
            FAFG+G+V P +A DPGLVYD +  DY+NYLCSL Y S +I L S    TC+S    L 
Sbjct: 619 AFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLS 678

Query: 661 PGDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFG 720
           PGDLNYPSF+V +   A   ++ +KRTVTNVG P  +Y V +  PKG+ V V+P+ L F 
Sbjct: 679 PGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQ 738

Query: 721 SLGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
              E+LSY V + A   +      SFG LVW+  KY VRSPIAVTW+
Sbjct: 739 KARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of HG10014553 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 6.4e-193
Identity = 387/768 (50.39%), Postives = 494/768 (64.32%), Query Frame = 0

Query: 7   WLLLSIMLAISSAAVDQQ-TYIIHMDTTKMITNNPE--QWYTTIIDSV-NELSSLDHNDE 66
           + LL++    SSA+     TYI+H+D     +  P    WYT+ + S+ +   S+ H  +
Sbjct: 8   FFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYD 67

Query: 67  EASNAAEILYLSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLGLQKDH--GLWNSSN 126
              +      L+++  + L      ++  P ++  LHTT SP+FLGL+     GL   S+
Sbjct: 68  TVFHGFS-ARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 127

Query: 127 LASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNCNKKLIGASAYIK 186
             SD++IG++DTG+WPE  SF D+GL PVP KWKG C A   F  S CN+KL+GA  +  
Sbjct: 128 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 187

Query: 187 GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMGVATGMSFTSRIA 246
           GYEA  G++NET  FRSPRDSDGHGTHTAS +AG  V  AS    A GVA GM+  +R+A
Sbjct: 188 GYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLA 247

Query: 247 AYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGVFV 306
           AYKVCW  GC  +DILAA D+AVADGVDV+SLS+GG    +Y D IAI AFGAI  G+FV
Sbjct: 248 AYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFV 307

Query: 307 SCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGSSLYVGKNINE-- 366
           S SAGN GP   TV N APW+ TV A   DR FP  VKLGNG++  G S+Y G  ++   
Sbjct: 308 SASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGR 367

Query: 367 -LPLVYNNT--AGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVKLAGGAGM 426
             PLVY  +   GDG  ++ C  GSLDP+ V GKIVLC+RG+NSR  KGE V+  GG GM
Sbjct: 368 MYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGM 427

Query: 427 ILINAQFEGEELFADPHVLPATTLGASAGIVILDYIA-SSESQAK----ASIAFEGTKYG 486
           I+ N  F+GE L AD HVLPAT++GAS G  I  YI+ SS+S++     A+I F+GT+ G
Sbjct: 428 IIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLG 487

Query: 487 SR-APRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIIS 546
            R AP VA+FS+RGP+   P+++KPDV APG+NILAAWP  + PS + SD RR  FNI+S
Sbjct: 488 IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILS 547

Query: 547 GTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADPF 606
           GTSM+CPHVSGLAALLK+AH DWSPAA++SAL+TTAY  DN    + D  +++G  +   
Sbjct: 548 GTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--ESTGNTSSVM 607

Query: 607 AFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQPG 666
            +GSGHVHP KA DPGLVYDIT  DYIN+LC+  Y  T I  ++R +  C   R     G
Sbjct: 608 DYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVG 667

Query: 667 DLNYPSFS-VFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGS 726
           +LNYPSFS VF +     +S  F RTVTNVG   S Y +KI  P+G TV V+PEKLSF  
Sbjct: 668 NLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRR 727

Query: 727 LGEKLSYQVGFVALGGKQDLG--EFSFGSLVWVSGKYAVRSPIAVTWQ 755
           +G+KLS+ V       K   G      G +VW  GK  V SP+ VT Q
Sbjct: 728 VGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of HG10014553 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 7.8e-191
Identity = 361/745 (48.46%), Postives = 492/745 (66.04%), Query Frame = 0

Query: 16  ISSAAVDQQTYIIHMDTTKMITNNP--EQWYTTIIDSVNELSSLDHNDEEASNAAEILYL 75
           +SS++ DQ TYI+HM  ++M ++      WY + + S+++ + L +  E A +      L
Sbjct: 22  VSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYENAIHGFS-TRL 81

Query: 76  STKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLGLQKDHG-LWNSSNLASDIIIGLLDT 135
           + ++ +SL    G ++  P    +LHTT +P FLGL +    L+  +   SD+++G+LDT
Sbjct: 82  TQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDT 141

Query: 136 GIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNCNKKLIGASAYIKGYEAIVGRLNET 195
           G+WPE  S+ D+G  P+PS WKG C+AG  F+ S CN+KLIGA  + +GYE+ +G ++E+
Sbjct: 142 GVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDES 201

Query: 196 GTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMGVATGMSFTSRIAAYKVCWPLGCAS 255
              RSPRD DGHGTHT+STAAG++V  ASL   A G A GM+  +R+A YKVCW  GC S
Sbjct: 202 KESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFS 261

Query: 256 ADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGVFVSCSAGNSGPSTS 315
           +DILAA+D A+AD V+VLS+SLGGG   +YRD +AI AF A++ G+ VSCSAGN+GPS+S
Sbjct: 262 SDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSS 321

Query: 316 TVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGSSLYVGKNINE--LPLVYNNTAGDG 375
           ++ N APWI TV A   DR FP    LGNG+ F G SL+ G+ + +  LP +Y   A + 
Sbjct: 322 SLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNA 381

Query: 376 QETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVKLAGGAGMILINAQFEGEELFAD 435
              N C TG+L P KV GKIV+C+RG+N+R  KG+ VK AGG GMIL N    GEEL AD
Sbjct: 382 TNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVAD 441

Query: 436 PHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTKYGSR-APRVAAFSSRGPSFVGP 495
            H+LPATT+G  AG +I  Y+ +++    ASI+  GT  G + +P VAAFSSRGP+ + P
Sbjct: 442 AHLLPATTVGEKAGDIIRHYV-TTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITP 501

Query: 496 DVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKSAH 555
           +++KPD+ APGVNILAAW     P+ L SD RRV FNIISGTSMSCPHVSGLAALLKS H
Sbjct: 502 NILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVH 561

Query: 556 KDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADPFAFGSGHVHPEKASDPGLVYD 615
            +WSPAA++SALMTTAY T      + D+  A+G P+ PF  G+GHV P  A++PGL+YD
Sbjct: 562 PEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPSTPFDHGAGHVSPTTATNPGLIYD 621

Query: 616 ITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQPGDLNYPSFSVFMKKKAKNVSI 675
           +T +DY+ +LC+L Y S QI  VSR   TC   +++    DLNYPSF+V +       + 
Sbjct: 622 LTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY-SVADLNYPSFAVNVDGVG---AY 681

Query: 676 TFKRTVTNVGIPRSDYTVKI-NNPKGITVIVKPEKLSFGSLGEKLSYQVGFVALGGKQDL 735
            + RTVT+VG     Y+VK+ +   G+ + V+P  L+F    EK SY V F     K   
Sbjct: 682 KYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPS- 741

Query: 736 GEFSFGSLVWVSGKYAVRSPIAVTW 754
           G  SFGS+ W  GK+ V SP+A++W
Sbjct: 742 GSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of HG10014553 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 4.0e-187
Identity = 362/775 (46.71%), Postives = 489/775 (63.10%), Query Frame = 0

Query: 6   VWLLLSIMLAISSAAVDQQ-----TYIIHMDTTKMIT--NNPEQWYTTIIDSVNELSSLD 65
           ++++LSI L    A    Q     TY+IHMD + M     N  QWY++ I+SV +  S  
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS-- 71

Query: 66  HNDEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQF 125
              EE  N   ILY            L+ ++   L +  G +A  P    +LHTT SP F
Sbjct: 72  --QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTF 131

Query: 126 LGL--QKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFS 185
           LGL  Q+   +W       D+++G+LDTGIWPE  SF D G+ PVP+ W+G C+ G +F 
Sbjct: 132 LGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFL 191

Query: 186 PSNCNKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNN 245
             NCN+K++GA  + +GYEA  G+++E   ++SPRD DGHGTHTA+T AG+ V  A+L  
Sbjct: 192 KRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 251

Query: 246 QAMGVATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRD 305
            A G A GM+  +R+AAYKVCW  GC S+DIL+A+D AVADGV VLS+SLGGG  ++ RD
Sbjct: 252 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 311

Query: 306 NIAIAAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQV 365
           +++IA FGA++ GVFVSCSAGN GP   ++ N +PWI TV AS  DR FP TVK+G  + 
Sbjct: 312 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 371

Query: 366 FEGSSLYVGKNI----NELPLVY-NNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNS 425
           F+G SLY G+ +     + PLVY    A     T+FC  G+LD   V GKIV+C+RG+  
Sbjct: 372 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 431

Query: 426 RTAKGEQVKLAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAK 485
           R  KG+ VK AGG GM+L N    GEEL AD H+LPA  +G   G +I  Y A +  +A 
Sbjct: 432 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQY-AMTSKKAT 491

Query: 486 ASIAFEGTKYGSR-APRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELES 545
           AS+   GT+ G + +P VAAFSSRGP+F+  +++KPD+ APGVNILAAW   ++PS L S
Sbjct: 492 ASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 551

Query: 546 DKRRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDV 605
           D RRV FNI+SGTSMSCPHVSG+AAL+KS H DWSPAA+KSALMTTAYV DN    ++D 
Sbjct: 552 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDA 611

Query: 606 GDASGGPADPFAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLT 665
             A+  P+ P+  G+GH+ P +A+DPGLVYDI PQ+Y  +LC+   + +Q+ + ++    
Sbjct: 612 SGAA--PSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 671

Query: 666 CSSKRTFLQPGDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVI 725
                    PG+LNYP+ S    +     ++T +RTVTNVG   S Y V ++  KG +V 
Sbjct: 672 TCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVT 731

Query: 726 VKPEKLSFGSLGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTW 754
           V+P+ L+F S  +KLSY V F     +  +    FG LVW S  + VRSP+ +TW
Sbjct: 732 VQPKTLNFTSKHQKLSYTVTFRT---RFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of HG10014553 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 1.5e-186
Identity = 362/739 (48.99%), Postives = 471/739 (63.73%), Query Frame = 0

Query: 23  QQTYII---HMDTTKMITNNPEQWYTTIIDSVNELSSLDHNDEEASNAAEILYLSTKKLN 82
           ++TYII   H D  +    +   WYT+    +N  SSL +    + +       ST+  +
Sbjct: 27  KKTYIIRVNHSDKPESFLTH-HDWYTS---QLNSESSLLYTYTTSFHGFSAYLDSTEADS 86

Query: 83  SLSKIRGFLAATPNELLQLHTTHSPQFLGLQKDHGLWNSSNLASDIIIGLLDTGIWPEHI 142
            LS     L    + L  LHTT +P+FLGL  + G+ +  + ++ +IIG+LDTG+WPE  
Sbjct: 87  LLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESR 146

Query: 143 SFQDKGLPPVPSKWKGICQAGAKFSPSNCNKKLIGASAYIKGYE-AIVGRLNETGTFRSP 202
           SF D  +P +PSKWKG C++G+ F    CNKKLIGA ++ KG++ A  G  +      SP
Sbjct: 147 SFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSP 206

Query: 203 RDSDGHGTHTASTAAGNIVNKASLNNQAMGVATGMSFTSRIAAYKVCWPLGCASADILAA 262
           RD DGHGTHT++TAAG+ V  AS    A G A GM+  +R+A YKVCW  GC  +DILAA
Sbjct: 207 RDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAA 266

Query: 263 MDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGVFVSCSAGNSGPSTSTVGNAA 322
           MD A+ DGVDVLSLSLGGG+  +YRD IAI AF A++ GVFVSCSAGNSGP+ ++V N A
Sbjct: 267 MDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVA 326

Query: 323 PWIMTVAASYTDRAFPTTVKLGNGQVFEGSSLY--VGKNINELPLVYNNTAGDGQETNFC 382
           PW+MTV A   DR FP    LGNG+   G SLY  VG     L LVYN   G+   +N C
Sbjct: 327 PWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNK--GNSSSSNLC 386

Query: 383 TTGSLDPSKVNGKIVLCERGLNSRTAKGEQVKLAGGAGMILINAQFEGEELFADPHVLPA 442
             GSLD S V GKIV+C+RG+N+R  KG  V+ AGG GMI+ N    GEEL AD H+LPA
Sbjct: 387 LPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPA 446

Query: 443 TTLGASAGIVILDYIASSESQAKASIAFEGTKYGSR-APRVAAFSSRGPSFVGPDVIKPD 502
             +G   G ++ +Y+  S+S+  A + F+GT    + +P VAAFSSRGP+ V P+++KPD
Sbjct: 447 IAVGKKTGDLLREYV-KSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPD 506

Query: 503 VTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPA 562
           V  PGVNILA W   + P+ L+ D RR  FNI+SGTSMSCPH+SGLA LLK+AH +WSP+
Sbjct: 507 VIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPS 566

Query: 563 AVKSALMTTAYVTDNKMSLISDVGDASGGPADPFAFGSGHVHPEKASDPGLVYDITPQDY 622
           A+KSALMTTAYV DN  + + D  D S   ++P+A GSGHV P+KA  PGLVYDI+ ++Y
Sbjct: 567 AIKSALMTTAYVLDNTNAPLHDAADNS--LSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 623 INYLCSLKYNSTQI-ALVSRGKLTCSSKRTFLQPGDLNYPSFSVFMKKKAKNVSITFKRT 682
           I +LCSL Y    I A+V R  + CS K  F  PG LNYPSFSV    K     + + R 
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVNCSKK--FSDPGQLNYPSFSVLFGGKR---VVRYTRE 686

Query: 683 VTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGSLGEKLSYQVGFVALGGKQDLGEFSFG 742
           VTNVG   S Y V +N    + + VKP KLSF S+GEK  Y V FV+  G     +  FG
Sbjct: 687 VTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 743 SLVWVSGKYAVRSPIAVTW 754
           S+ W + ++ VRSP+A +W
Sbjct: 747 SITWSNPQHEVRSPVAFSW 751

BLAST of HG10014553 vs. ExPASy TrEMBL
Match: A0A1S3C0J8 (subtilisin-like protease SBT1.1 OS=Cucumis melo OX=3656 GN=LOC103495084 PE=3 SV=1)

HSP 1 Score: 1298.5 bits (3359), Expect = 0.0e+00
Identity = 665/766 (86.81%), Postives = 698/766 (91.12%), Query Frame = 0

Query: 1   MGFREVWLLLSIMLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSLDHN 60
           MGFREVW +LSIMLAISSA VDQQTYIIHMDTTKM+T NPEQWYT IIDSVNELSSLD N
Sbjct: 1   MGFREVW-VLSIMLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDDN 60

Query: 61  DEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLG 120
            EEASNAAEILY            L++KKL+SLSKI GFLAATPNELLQLHTTHSPQFLG
Sbjct: 61  -EEASNAAEILYVYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLG 120

Query: 121 LQKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNC 180
           LQ+DHGLWN SNLASDIIIGLLDTGIWPEHISFQDKGL  VP KWKGICQ G +FS SNC
Sbjct: 121 LQRDHGLWNFSNLASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNC 180

Query: 181 NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMG 240
           NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAG+IV+ AS  NQ MG
Sbjct: 181 NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMG 240

Query: 241 VATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAI 300
           VA+GM FTSRI AYKVCWPLGCA+ADILAAMDSAVADGVDVLSLSLGGG+ SFY+DNIAI
Sbjct: 241 VASGMRFTSRIVAYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAI 300

Query: 301 AAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGS 360
           AAFGAIQ GVFVSCSAGNSGPS STVGNAAPWIMTVAASYTDR FPTTVKLGNGQVFEGS
Sbjct: 301 AAFGAIQKGVFVSCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGS 360

Query: 361 SLYVGKNINELPLVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVK 420
           SLY GK+INELPLVYNNTAGDGQETN C  GSLDPS V GKIV+CERG  SRT KGEQVK
Sbjct: 361 SLYYGKSINELPLVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVK 420

Query: 421 LAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTK 480
           LAGG GMILIN QFEGEELFADPHVLPATTLGASAG  ILDYIASS++QAKASI FEGTK
Sbjct: 421 LAGGTGMILINTQFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTK 480

Query: 481 YGSRAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNII 540
           YGS+APRVAAFSSRGPS VGPDVIKPDVTAPGVNILAAWPPIVSPSEL SD RRV+FNII
Sbjct: 481 YGSQAPRVAAFSSRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNII 540

Query: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADP 600
           SGTSMSCPHVSGLAALLKSAH DWSPAA+KSALMTTAYVTD+KMSLISDVG A+G PA P
Sbjct: 541 SGTSMSCPHVSGLAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATP 600

Query: 601 FAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQP 660
           F FGSGHV PEKASDPGL+YDITPQDYINYLCSLKYNS+QIALVSRG LTCSSKRT ++P
Sbjct: 601 FTFGSGHVDPEKASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKP 660

Query: 661 GDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGS 720
           GDLNYPSFSVFMKKKAK VSIT KRTVTNVGI RSDYTVKINNPKG+TVIVKPEKLSFGS
Sbjct: 661 GDLNYPSFSVFMKKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGS 720

Query: 721 LGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
           LGE+LSY+V FV+LGGK+ L +FSFGSLVW+SGKYAVRSPI VTWQ
Sbjct: 721 LGEQLSYKVSFVSLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 764

BLAST of HG10014553 vs. ExPASy TrEMBL
Match: A0A5D3DZC9 (Subtilisin-like protease SBT1.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G001390 PE=3 SV=1)

HSP 1 Score: 1282.7 bits (3318), Expect = 0.0e+00
Identity = 655/754 (86.87%), Postives = 687/754 (91.11%), Query Frame = 0

Query: 13  MLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSLDHNDEEASNAAEILY 72
           MLAISSA VDQQTYIIHMDTTKM+T NPEQWYT IIDSVNELSSLD N EEASNAAEILY
Sbjct: 1   MLAISSAVVDQQTYIIHMDTTKMVTPNPEQWYTDIIDSVNELSSLDDN-EEASNAAEILY 60

Query: 73  ------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLGLQKDHGLWNSSN 132
                       L++KKL+SLSKI GFLAATPNELLQLHTTHSPQFLGLQ+DHGLWN SN
Sbjct: 61  VYKTALSGFAAKLTSKKLHSLSKIPGFLAATPNELLQLHTTHSPQFLGLQRDHGLWNFSN 120

Query: 133 LASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNCNKKLIGASAYIK 192
           LASDIIIGLLDTGIWPEHISFQDKGL  VP KWKGICQ G +FS SNCNKKLIGASAYIK
Sbjct: 121 LASDIIIGLLDTGIWPEHISFQDKGLSSVPLKWKGICQTGPRFSSSNCNKKLIGASAYIK 180

Query: 193 GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMGVATGMSFTSRIA 252
           GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAG+IV+ AS  NQ MGVA+GM FTSRI 
Sbjct: 181 GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGSIVDNASFYNQGMGVASGMRFTSRIV 240

Query: 253 AYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGVFV 312
           AYKVCWPLGCA+ADILAAMDSAVADGVDVLSLSLGGG+ SFY+DNIAIAAFGAIQ GVFV
Sbjct: 241 AYKVCWPLGCANADILAAMDSAVADGVDVLSLSLGGGSSSFYKDNIAIAAFGAIQKGVFV 300

Query: 313 SCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGSSLYVGKNINELP 372
           SCSAGNSGPS STVGNAAPWIMTVAASYTDR FPTTVKLGNGQVFEGSSLY GK+INELP
Sbjct: 301 SCSAGNSGPSPSTVGNAAPWIMTVAASYTDRTFPTTVKLGNGQVFEGSSLYYGKSINELP 360

Query: 373 LVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVKLAGGAGMILINA 432
           LVYNNTAGDGQETN C  GSLDPS V GKIV+CERG  SRT KGEQVKLAGG GMILIN 
Sbjct: 361 LVYNNTAGDGQETNVCIAGSLDPSMVKGKIVICERGTISRTEKGEQVKLAGGTGMILINT 420

Query: 433 QFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTKYGSRAPRVAAFS 492
           QFEGEELFADPHVLPATTLGASAG  ILDYIASS++QAKASI FEGTKYGS+APRVAAFS
Sbjct: 421 QFEGEELFADPHVLPATTLGASAGKAILDYIASSKTQAKASIVFEGTKYGSQAPRVAAFS 480

Query: 493 SRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSG 552
           SRGPS VGPDVIKPDVTAPGVNILAAWPPIVSPSEL SD RRV+FNIISGTSMSCPHVSG
Sbjct: 481 SRGPSLVGPDVIKPDVTAPGVNILAAWPPIVSPSELASDTRRVMFNIISGTSMSCPHVSG 540

Query: 553 LAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADPFAFGSGHVHPEK 612
           LAALLKSAH DWSPAA+KSALMTTAYVTD+KMSLISDVG A+G PA PF FGSGHV PEK
Sbjct: 541 LAALLKSAHNDWSPAAIKSALMTTAYVTDSKMSLISDVGQANGEPATPFTFGSGHVDPEK 600

Query: 613 ASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQPGDLNYPSFSVFM 672
           ASDPGL+YDITPQDYINYLCSLKYNS+QIALVSRG LTCSSKRT ++PGDLNYPSFSVFM
Sbjct: 601 ASDPGLIYDITPQDYINYLCSLKYNSSQIALVSRGNLTCSSKRTVVKPGDLNYPSFSVFM 660

Query: 673 KKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGSLGEKLSYQVGFV 732
           KKKAK VSIT KRTVTNVGI RSDYTVKINNPKG+TVIVKPEKLSFGSLGE+LSY+V FV
Sbjct: 661 KKKAKKVSITLKRTVTNVGITRSDYTVKINNPKGVTVIVKPEKLSFGSLGEQLSYKVSFV 720

Query: 733 ALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
           +LGGK+ L +FSFGSLVW+SGKYAVRSPI VTWQ
Sbjct: 721 SLGGKEALDKFSFGSLVWISGKYAVRSPIVVTWQ 753

BLAST of HG10014553 vs. ExPASy TrEMBL
Match: A0A6J1JPC3 (subtilisin-like protease SBT1.1 OS=Cucurbita maxima OX=3661 GN=LOC111487676 PE=3 SV=1)

HSP 1 Score: 1220.3 bits (3156), Expect = 0.0e+00
Identity = 624/766 (81.46%), Postives = 671/766 (87.60%), Query Frame = 0

Query: 1   MGFREVWLLLSIMLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSLDHN 60
           MGFREV L LSI LA S+AAVDQQ+YIIHMDTTKM   +PEQWYT +IDS+N++SSLD +
Sbjct: 1   MGFREVCLFLSIFLATSAAAVDQQSYIIHMDTTKMAAPSPEQWYTALIDSINKISSLD-D 60

Query: 61  DEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLG 120
            EEAS+AA+ILY            LSTKKL+SLSK  GFLAATPNELLQLHTTHSPQFLG
Sbjct: 61  QEEASSAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLG 120

Query: 121 LQKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNC 180
           LQ++HGLWNSSNLASDI+IGLLDTGIWPEHISFQDKGLPPVP KWKG CQAG KFSPSNC
Sbjct: 121 LQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNC 180

Query: 181 NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMG 240
           N+KLIGA+AYIKGYEAIVGRLN TGTFRSPRDSDGHGTHTASTAAGNIVNKAS  NQAMG
Sbjct: 181 NRKLIGATAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMG 240

Query: 241 VATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAI 300
           VATGM FTSRIAAYKVCW  GCA+ADILAA+D AVADGVDVLSLSLGG A +FY+D+IAI
Sbjct: 241 VATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIAI 300

Query: 301 AAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGS 360
           A FGA++NGVFVSCSAGNSGPS+STV N APWIMTVAASYTDR FP TVKLGNGQVFEGS
Sbjct: 301 ATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRTFPATVKLGNGQVFEGS 360

Query: 361 SLYVGKNINELPLVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVK 420
           SLY G +I +LPLVYNNTAG G+E N CT GSL PS V GKIV+CERG NSRTAKGEQVK
Sbjct: 361 SLYSGNSIGQLPLVYNNTAG-GEEANVCTAGSLVPSLVKGKIVVCERGTNSRTAKGEQVK 420

Query: 421 LAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTK 480
           LAGGAGMILIN Q EGEELFADPHVLPA  LGASAG  I+ YI+SS+ Q KA IAFEGTK
Sbjct: 421 LAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGTK 480

Query: 481 YGSRAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNII 540
           +G+RAPRVAAFSSRGPS + PDVIKPDVTAPGVNILAAWP I SPSELESDKRRVLFNII
Sbjct: 481 FGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSELESDKRRVLFNII 540

Query: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADP 600
           SGTSMSCPHVSGLAALLKSAHKDWSPAA+KSALMTTAY  DN+MS ISDVG  SG PA+P
Sbjct: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSESGKPANP 600

Query: 601 FAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQP 660
           FAFGSGHV PEKASDPGL+YDITPQDY+NY CSL YNSTQI LVSRG  TC SKR   QP
Sbjct: 601 FAFGSGHVDPEKASDPGLIYDITPQDYLNYFCSLNYNSTQIGLVSRGNFTCPSKRRVGQP 660

Query: 661 GDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGS 720
           G+LNYPSFSVFMKKKAKNVS+T KRTVTNVG PRSDYTVKI NPKGI + V+PEKLSF  
Sbjct: 661 GNLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYTVKIKNPKGIGIRVEPEKLSFRR 720

Query: 721 LGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
            G+KLSYQV FVALG ++ LG FSFGSLVWVSGKY VRSPIAVTW+
Sbjct: 721 YGQKLSYQVSFVALGKREGLGGFSFGSLVWVSGKYGVRSPIAVTWK 764

BLAST of HG10014553 vs. ExPASy TrEMBL
Match: A0A6J1FMR4 (subtilisin-like protease SBT1.1 OS=Cucurbita moschata OX=3662 GN=LOC111446877 PE=3 SV=1)

HSP 1 Score: 1216.4 bits (3146), Expect = 0.0e+00
Identity = 622/766 (81.20%), Postives = 669/766 (87.34%), Query Frame = 0

Query: 1   MGFREVWLLLSIMLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSLDHN 60
           MGFREV L LSI LA S+AAVDQQ+YIIHMDTTKM    PEQWYT +IDS+NE+SSL+ +
Sbjct: 1   MGFREVCLFLSIFLATSTAAVDQQSYIIHMDTTKMAAPTPEQWYTALIDSINEISSLE-D 60

Query: 61  DEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLG 120
            EEASNAA+ILY            LSTKKL+SLSK  GFLAATPNELLQLHTTHSPQFLG
Sbjct: 61  QEEASNAAQILYVYKTAISGFAAKLSTKKLHSLSKTPGFLAATPNELLQLHTTHSPQFLG 120

Query: 121 LQKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNC 180
           LQ++HGLWNSSNLASDI+IGLLDTGIWPEHISFQDKGLPPVP KWKG CQAG KFSPSNC
Sbjct: 121 LQREHGLWNSSNLASDIVIGLLDTGIWPEHISFQDKGLPPVPKKWKGTCQAGQKFSPSNC 180

Query: 181 NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMG 240
           N+KLIGA AYIKGYEAIVGRLN TGTFRSPRDSDGHGTHTASTAAGNIVNKAS  NQAMG
Sbjct: 181 NRKLIGAIAYIKGYEAIVGRLNATGTFRSPRDSDGHGTHTASTAAGNIVNKASFFNQAMG 240

Query: 241 VATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAI 300
           VATGM FTSRIAAYKVCW  GCA+ADILAA+D AVADGVDVLSLSLGG A +FY+D+IAI
Sbjct: 241 VATGMRFTSRIAAYKVCWTEGCANADILAAIDRAVADGVDVLSLSLGGSASAFYKDDIAI 300

Query: 301 AAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGS 360
           A FGA++NGVFVSCSAGNSGPS+STV N APWIMTVAASYTDR+FP TVKLGNGQVFEGS
Sbjct: 301 ATFGAVRNGVFVSCSAGNSGPSSSTVSNIAPWIMTVAASYTDRSFPATVKLGNGQVFEGS 360

Query: 361 SLYVGKNINELPLVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVK 420
           SLY G NI +LPLVYNNTAG G++ N CT GSL PS V GKIV+CERG NSRTAKGEQVK
Sbjct: 361 SLYSGNNIGQLPLVYNNTAG-GEDANVCTAGSLVPSMVKGKIVVCERGTNSRTAKGEQVK 420

Query: 421 LAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTK 480
           LAGGAGMILIN Q EGEELFADPHVLPA  LGASAG  I+ YI+SS+ Q KA IAFEGTK
Sbjct: 421 LAGGAGMILINTQLEGEELFADPHVLPAANLGASAGQAIIRYISSSKHQPKALIAFEGTK 480

Query: 481 YGSRAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNII 540
           +G+RAPRVAAFSSRGPS + PDVIKPDVTAPGVNILAAWP I SPSE+ESDKRRVLFN+I
Sbjct: 481 FGNRAPRVAAFSSRGPSLIAPDVIKPDVTAPGVNILAAWPLIASPSEVESDKRRVLFNVI 540

Query: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADP 600
           SGTSMSCPHVSGLAALLKSAHKDWSPAA+KSALMTTAY  DN+MS ISDVG ASG PA+P
Sbjct: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAIKSALMTTAYTNDNRMSPISDVGSASGKPANP 600

Query: 601 FAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQP 660
           FAFGSGHV PEKASDPGL+YDITPQDY+NYLCSL YNSTQI LVSRG  TC SKR   Q 
Sbjct: 601 FAFGSGHVDPEKASDPGLIYDITPQDYLNYLCSLNYNSTQIGLVSRGNFTCPSKRRVGQA 660

Query: 661 GDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGS 720
           G LNYPSFSVFMKKKAKNVS+T KRTVTNVG PRSDY+VKI NPKGI + VKPEKLSF  
Sbjct: 661 GKLNYPSFSVFMKKKAKNVSVTLKRTVTNVGRPRSDYSVKIKNPKGIGISVKPEKLSFRR 720

Query: 721 LGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
            G+KLSYQV FVALG ++ +  FSFGSLVWVSG YAVRSPIAVTW+
Sbjct: 721 YGQKLSYQVSFVALGKREGVSGFSFGSLVWVSGTYAVRSPIAVTWK 764

BLAST of HG10014553 vs. ExPASy TrEMBL
Match: A0A5A7UKU0 (Subtilisin-like protease SBT1.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold186G002890 PE=3 SV=1)

HSP 1 Score: 1207.2 bits (3122), Expect = 0.0e+00
Identity = 613/744 (82.39%), Postives = 654/744 (87.90%), Query Frame = 0

Query: 13  MLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSLD--HNDEEASNAAEI 72
           ML  S  A+DQQ+YI+HMDTTKM T NPEQWYT II SVN+LSSLD  +N+E+A N AEI
Sbjct: 1   MLTTSIVAMDQQSYIVHMDTTKMATTNPEQWYTAIIHSVNKLSSLDANNNEEQALNIAEI 60

Query: 73  LYLSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLGLQKDHGLWNSSNLASDIIIGLL 132
           LYLS+K L+ LSK+ GF+AA+PNELLQLHTTHSP+FLGLQ+ HGLWNSSNLASDIIIG+L
Sbjct: 61  LYLSSKNLHFLSKVPGFIAASPNELLQLHTTHSPKFLGLQRGHGLWNSSNLASDIIIGVL 120

Query: 133 DTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNCNKKLIGASAYIKGYEAIVGRLN 192
           DTGIWPEHISFQDK LPPVPSKWKGICQ G  FS SNCNKKLIGA  +I+ YEA VGRLN
Sbjct: 121 DTGIWPEHISFQDKALPPVPSKWKGICQTGPSFSLSNCNKKLIGARTFIQAYEAAVGRLN 180

Query: 193 ETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMGVATGMSFTSRIAAYKVCWPLGC 252
            TGTFRS RDSDGHGTHTASTAAGN VN+AS  NQ MGVATGM FTSRIAAYKVCWP GC
Sbjct: 181 ATGTFRSARDSDGHGTHTASTAAGNFVNRASFYNQGMGVATGMRFTSRIAAYKVCWPEGC 240

Query: 253 ASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGVFVSCSAGNSGPS 312
           ASADILAAMD AVADGVDVLS+SLGGG+   Y D IAIAAFGAIQ GVFVSCSAGNSGP 
Sbjct: 241 ASADILAAMDHAVADGVDVLSISLGGGSSIIYSDQIAIAAFGAIQKGVFVSCSAGNSGPF 300

Query: 313 TSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGSSLYVGKNINELPLVYNNTAGDG 372
            STVGN APW+MTVAASYTDR FPTTV+LGNG VFEGSSLY GKN+ E PLVYNNTAGDG
Sbjct: 301 ISTVGNVAPWVMTVAASYTDRTFPTTVRLGNGMVFEGSSLYFGKNLKETPLVYNNTAGDG 360

Query: 373 QETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVKLAGGAGMILINAQFEGEELFAD 432
           +ETNFCT GSLDP+ V GKI +CERG NSRT KGEQVKLAGGAGMILIN   EGE+L AD
Sbjct: 361 RETNFCTPGSLDPTMVKGKIAVCERGTNSRTEKGEQVKLAGGAGMILINTILEGEDLLAD 420

Query: 433 PHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTKYGSRAPRVAAFSSRGPSFVGPD 492
            HVLPAT++G SAG  IL+YIASS+ QAKASI F+GTKYGSRAPRVAAFSSRGPSF  P 
Sbjct: 421 SHVLPATSVGVSAGKSILNYIASSKRQAKASIMFKGTKYGSRAPRVAAFSSRGPSFYKPY 480

Query: 493 VIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKSAHK 552
           VIKPD+TAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKS HK
Sbjct: 481 VIKPDITAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKSVHK 540

Query: 553 DWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADPFAFGSGHVHPEKASDPGLVYDI 612
           DWSPAA+KSALMTTAYVTDNK  LISDVG ASGGPADPFAFGSGHV PEKASDPGLVYDI
Sbjct: 541 DWSPAAIKSALMTTAYVTDNKNHLISDVGRASGGPADPFAFGSGHVDPEKASDPGLVYDI 600

Query: 613 TPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQPGDLNYPSFSVFMKKKAKNVSIT 672
            PQDYI YLCSLKYNSTQIALVSRGK TCSSKRTF QPGDLNYPSFSVFM KK KNV+ T
Sbjct: 601 APQDYITYLCSLKYNSTQIALVSRGKFTCSSKRTFFQPGDLNYPSFSVFM-KKGKNVNST 660

Query: 673 FKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGSLGEKLSYQVGFVALGGKQDLGE 732
           FKRTVTNVGIPRSDYTV+I NPKGI +IVKPEKLSF  LGEKLSY+V FVALG ++ L +
Sbjct: 661 FKRTVTNVGIPRSDYTVRIYNPKGIRIIVKPEKLSFVKLGEKLSYKVSFVALGKRKSLDD 720

Query: 733 FSFGSLVWVSGKYAVRSPIAVTWQ 755
           FSFGSLVW SG Y VRSPIAVTWQ
Sbjct: 721 FSFGSLVWHSGTYVVRSPIAVTWQ 743

BLAST of HG10014553 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 795.8 bits (2054), Expect = 3.0e-230
Identity = 418/767 (54.50%), Postives = 533/767 (69.49%), Query Frame = 0

Query: 1   MGFREVWLLLSIMLAISSAAVDQQTYIIHMDTTKMITNNPEQWYTTIIDSVNELSSLDHN 60
           M FR   +   ++   S+ +  +QTY+IH      +T + +   T++    N L + + N
Sbjct: 19  MFFRSFIVFFFLIFFASNVSSRKQTYVIH-----TVTTSTKHIVTSLF---NSLQTENIN 78

Query: 61  DEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLG 120
           D++ S   EI Y            L+  +L+++   +GF++A P+ELL LHTT+S +FLG
Sbjct: 79  DDDFS-LPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLG 138

Query: 121 LQKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNC 180
           L+   GLWN ++L+SD+IIGL+DTGI PEH+SF+D  + PVPS+W+G C  G  FS S C
Sbjct: 139 LEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSEC 198

Query: 181 NKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMG 240
           NKK+IGASA+ KGYE+IVG++NET  FRS RD+ GHGTHTASTAAG+IV KA+   QA G
Sbjct: 199 NKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKG 258

Query: 241 VATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAI 300
           +A+GM FTSRIAAYK CW LGCAS D++AA+D A+ DGVDV+SLSLGG +  FY D IAI
Sbjct: 259 LASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAI 318

Query: 301 AAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGS 360
           A FGA+Q  +FVSCSAGNSGP+ STV N APW+MTVAASYTDR FP  V++GN +   GS
Sbjct: 319 AGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGS 378

Query: 361 SLYVGKNINELPLVYNNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVK 420
           SLY GK++  LPL +N TAG+     FC   SL    V GKIV+C RG + RTAKGE+VK
Sbjct: 379 SLYKGKSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASGRTAKGEEVK 438

Query: 421 LAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTK 480
            +GGA M+L++ + EGEEL ADPHVLPA +LG S G  +L+Y+A + + A AS+ F GT 
Sbjct: 439 RSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGA-ANATASVRFRGTA 498

Query: 481 YGSRAPRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNII 540
           YG+ AP VAAFSSRGPS  GP++ KPD+ APG+NILA W P  SPS L SD RRV FNII
Sbjct: 499 YGATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNII 558

Query: 541 SGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASG-GPAD 600
           SGTSM+CPH+SG+AAL+KS H DWSPA +KSA+MTTA +TDN+   I D G A     A 
Sbjct: 559 SGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAAT 618

Query: 601 PFAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQ 660
            FAFG+G+V P +A DPGLVYD +  DY+NYLCSL Y S +I L S    TC+S    L 
Sbjct: 619 AFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLS 678

Query: 661 PGDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFG 720
           PGDLNYPSF+V +   A   ++ +KRTVTNVG P  +Y V +  PKG+ V V+P+ L F 
Sbjct: 679 PGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQ 738

Query: 721 SLGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTWQ 755
              E+LSY V + A   +      SFG LVW+  KY VRSPIAVTW+
Sbjct: 739 KARERLSYTVTYDAEASRNS-SSSSFGVLVWICDKYNVRSPIAVTWE 774

BLAST of HG10014553 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 675.6 bits (1742), Expect = 4.5e-194
Identity = 387/768 (50.39%), Postives = 494/768 (64.32%), Query Frame = 0

Query: 7   WLLLSIMLAISSAAVDQQ-TYIIHMDTTKMITNNPE--QWYTTIIDSV-NELSSLDHNDE 66
           + LL++    SSA+     TYI+H+D     +  P    WYT+ + S+ +   S+ H  +
Sbjct: 8   FFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYD 67

Query: 67  EASNAAEILYLSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLGLQKDH--GLWNSSN 126
              +      L+++  + L      ++  P ++  LHTT SP+FLGL+     GL   S+
Sbjct: 68  TVFHGFS-ARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 127

Query: 127 LASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNCNKKLIGASAYIK 186
             SD++IG++DTG+WPE  SF D+GL PVP KWKG C A   F  S CN+KL+GA  +  
Sbjct: 128 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 187

Query: 187 GYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMGVATGMSFTSRIA 246
           GYEA  G++NET  FRSPRDSDGHGTHTAS +AG  V  AS    A GVA GM+  +R+A
Sbjct: 188 GYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLA 247

Query: 247 AYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGVFV 306
           AYKVCW  GC  +DILAA D+AVADGVDV+SLS+GG    +Y D IAI AFGAI  G+FV
Sbjct: 248 AYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFV 307

Query: 307 SCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGSSLYVGKNINE-- 366
           S SAGN GP   TV N APW+ TV A   DR FP  VKLGNG++  G S+Y G  ++   
Sbjct: 308 SASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGR 367

Query: 367 -LPLVYNNT--AGDGQETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVKLAGGAGM 426
             PLVY  +   GDG  ++ C  GSLDP+ V GKIVLC+RG+NSR  KGE V+  GG GM
Sbjct: 368 MYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGM 427

Query: 427 ILINAQFEGEELFADPHVLPATTLGASAGIVILDYIA-SSESQAK----ASIAFEGTKYG 486
           I+ N  F+GE L AD HVLPAT++GAS G  I  YI+ SS+S++     A+I F+GT+ G
Sbjct: 428 IIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLG 487

Query: 487 SR-APRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIIS 546
            R AP VA+FS+RGP+   P+++KPDV APG+NILAAWP  + PS + SD RR  FNI+S
Sbjct: 488 IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILS 547

Query: 547 GTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADPF 606
           GTSM+CPHVSGLAALLK+AH DWSPAA++SAL+TTAY  DN    + D  +++G  +   
Sbjct: 548 GTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMD--ESTGNTSSVM 607

Query: 607 AFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQPG 666
            +GSGHVHP KA DPGLVYDIT  DYIN+LC+  Y  T I  ++R +  C   R     G
Sbjct: 608 DYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVG 667

Query: 667 DLNYPSFS-VFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGS 726
           +LNYPSFS VF +     +S  F RTVTNVG   S Y +KI  P+G TV V+PEKLSF  
Sbjct: 668 NLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRR 727

Query: 727 LGEKLSYQVGFVALGGKQDLG--EFSFGSLVWVSGKYAVRSPIAVTWQ 755
           +G+KLS+ V       K   G      G +VW  GK  V SP+ VT Q
Sbjct: 728 VGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQ 772

BLAST of HG10014553 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 668.7 bits (1724), Expect = 5.5e-192
Identity = 361/745 (48.46%), Postives = 492/745 (66.04%), Query Frame = 0

Query: 16  ISSAAVDQQTYIIHMDTTKMITNNP--EQWYTTIIDSVNELSSLDHNDEEASNAAEILYL 75
           +SS++ DQ TYI+HM  ++M ++      WY + + S+++ + L +  E A +      L
Sbjct: 22  VSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYENAIHGFS-TRL 81

Query: 76  STKKLNSLSKIRGFLAATPNELLQLHTTHSPQFLGLQKDHG-LWNSSNLASDIIIGLLDT 135
           + ++ +SL    G ++  P    +LHTT +P FLGL +    L+  +   SD+++G+LDT
Sbjct: 82  TQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDT 141

Query: 136 GIWPEHISFQDKGLPPVPSKWKGICQAGAKFSPSNCNKKLIGASAYIKGYEAIVGRLNET 195
           G+WPE  S+ D+G  P+PS WKG C+AG  F+ S CN+KLIGA  + +GYE+ +G ++E+
Sbjct: 142 GVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDES 201

Query: 196 GTFRSPRDSDGHGTHTASTAAGNIVNKASLNNQAMGVATGMSFTSRIAAYKVCWPLGCAS 255
              RSPRD DGHGTHT+STAAG++V  ASL   A G A GM+  +R+A YKVCW  GC S
Sbjct: 202 KESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFS 261

Query: 256 ADILAAMDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGVFVSCSAGNSGPSTS 315
           +DILAA+D A+AD V+VLS+SLGGG   +YRD +AI AF A++ G+ VSCSAGN+GPS+S
Sbjct: 262 SDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSS 321

Query: 316 TVGNAAPWIMTVAASYTDRAFPTTVKLGNGQVFEGSSLYVGKNINE--LPLVYNNTAGDG 375
           ++ N APWI TV A   DR FP    LGNG+ F G SL+ G+ + +  LP +Y   A + 
Sbjct: 322 SLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNA 381

Query: 376 QETNFCTTGSLDPSKVNGKIVLCERGLNSRTAKGEQVKLAGGAGMILINAQFEGEELFAD 435
              N C TG+L P KV GKIV+C+RG+N+R  KG+ VK AGG GMIL N    GEEL AD
Sbjct: 382 TNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVAD 441

Query: 436 PHVLPATTLGASAGIVILDYIASSESQAKASIAFEGTKYGSR-APRVAAFSSRGPSFVGP 495
            H+LPATT+G  AG +I  Y+ +++    ASI+  GT  G + +P VAAFSSRGP+ + P
Sbjct: 442 AHLLPATTVGEKAGDIIRHYV-TTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITP 501

Query: 496 DVIKPDVTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKSAH 555
           +++KPD+ APGVNILAAW     P+ L SD RRV FNIISGTSMSCPHVSGLAALLKS H
Sbjct: 502 NILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVH 561

Query: 556 KDWSPAAVKSALMTTAYVTDNKMSLISDVGDASGGPADPFAFGSGHVHPEKASDPGLVYD 615
            +WSPAA++SALMTTAY T      + D+  A+G P+ PF  G+GHV P  A++PGL+YD
Sbjct: 562 PEWSPAAIRSALMTTAYKTYKDGKPLLDI--ATGKPSTPFDHGAGHVSPTTATNPGLIYD 621

Query: 616 ITPQDYINYLCSLKYNSTQIALVSRGKLTCSSKRTFLQPGDLNYPSFSVFMKKKAKNVSI 675
           +T +DY+ +LC+L Y S QI  VSR   TC   +++    DLNYPSF+V +       + 
Sbjct: 622 LTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY-SVADLNYPSFAVNVDGVG---AY 681

Query: 676 TFKRTVTNVGIPRSDYTVKI-NNPKGITVIVKPEKLSFGSLGEKLSYQVGFVALGGKQDL 735
            + RTVT+VG     Y+VK+ +   G+ + V+P  L+F    EK SY V F     K   
Sbjct: 682 KYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPS- 741

Query: 736 GEFSFGSLVWVSGKYAVRSPIAVTW 754
           G  SFGS+ W  GK+ V SP+A++W
Sbjct: 742 GSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of HG10014553 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 656.4 bits (1692), Expect = 2.8e-188
Identity = 362/775 (46.71%), Postives = 489/775 (63.10%), Query Frame = 0

Query: 6   VWLLLSIMLAISSAAVDQQ-----TYIIHMDTTKMIT--NNPEQWYTTIIDSVNELSSLD 65
           ++++LSI L    A    Q     TY+IHMD + M     N  QWY++ I+SV +  S  
Sbjct: 12  LFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS-- 71

Query: 66  HNDEEASNAAEILY------------LSTKKLNSLSKIRGFLAATPNELLQLHTTHSPQF 125
              EE  N   ILY            L+ ++   L +  G +A  P    +LHTT SP F
Sbjct: 72  --QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTF 131

Query: 126 LGL--QKDHGLWNSSNLASDIIIGLLDTGIWPEHISFQDKGLPPVPSKWKGICQAGAKFS 185
           LGL  Q+   +W       D+++G+LDTGIWPE  SF D G+ PVP+ W+G C+ G +F 
Sbjct: 132 LGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFL 191

Query: 186 PSNCNKKLIGASAYIKGYEAIVGRLNETGTFRSPRDSDGHGTHTASTAAGNIVNKASLNN 245
             NCN+K++GA  + +GYEA  G+++E   ++SPRD DGHGTHTA+T AG+ V  A+L  
Sbjct: 192 KRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFG 251

Query: 246 QAMGVATGMSFTSRIAAYKVCWPLGCASADILAAMDSAVADGVDVLSLSLGGGAGSFYRD 305
            A G A GM+  +R+AAYKVCW  GC S+DIL+A+D AVADGV VLS+SLGGG  ++ RD
Sbjct: 252 FAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRD 311

Query: 306 NIAIAAFGAIQNGVFVSCSAGNSGPSTSTVGNAAPWIMTVAASYTDRAFPTTVKLGNGQV 365
           +++IA FGA++ GVFVSCSAGN GP   ++ N +PWI TV AS  DR FP TVK+G  + 
Sbjct: 312 SLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRT 371

Query: 366 FEGSSLYVGKNI----NELPLVY-NNTAGDGQETNFCTTGSLDPSKVNGKIVLCERGLNS 425
           F+G SLY G+ +     + PLVY    A     T+FC  G+LD   V GKIV+C+RG+  
Sbjct: 372 FKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTP 431

Query: 426 RTAKGEQVKLAGGAGMILINAQFEGEELFADPHVLPATTLGASAGIVILDYIASSESQAK 485
           R  KG+ VK AGG GM+L N    GEEL AD H+LPA  +G   G +I  Y A +  +A 
Sbjct: 432 RVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQY-AMTSKKAT 491

Query: 486 ASIAFEGTKYGSR-APRVAAFSSRGPSFVGPDVIKPDVTAPGVNILAAWPPIVSPSELES 545
           AS+   GT+ G + +P VAAFSSRGP+F+  +++KPD+ APGVNILAAW   ++PS L S
Sbjct: 492 ASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSS 551

Query: 546 DKRRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPAAVKSALMTTAYVTDNKMSLISDV 605
           D RRV FNI+SGTSMSCPHVSG+AAL+KS H DWSPAA+KSALMTTAYV DN    ++D 
Sbjct: 552 DPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDA 611

Query: 606 GDASGGPADPFAFGSGHVHPEKASDPGLVYDITPQDYINYLCSLKYNSTQIALVSRGKLT 665
             A+  P+ P+  G+GH+ P +A+DPGLVYDI PQ+Y  +LC+   + +Q+ + ++    
Sbjct: 612 SGAA--PSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 671

Query: 666 CSSKRTFLQPGDLNYPSFSVFMKKKAKNVSITFKRTVTNVGIPRSDYTVKINNPKGITVI 725
                    PG+LNYP+ S    +     ++T +RTVTNVG   S Y V ++  KG +V 
Sbjct: 672 TCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVT 731

Query: 726 VKPEKLSFGSLGEKLSYQVGFVALGGKQDLGEFSFGSLVWVSGKYAVRSPIAVTW 754
           V+P+ L+F S  +KLSY V F     +  +    FG LVW S  + VRSP+ +TW
Sbjct: 732 VQPKTLNFTSKHQKLSYTVTFRT---RFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of HG10014553 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 654.4 bits (1687), Expect = 1.1e-187
Identity = 362/739 (48.99%), Postives = 471/739 (63.73%), Query Frame = 0

Query: 23  QQTYII---HMDTTKMITNNPEQWYTTIIDSVNELSSLDHNDEEASNAAEILYLSTKKLN 82
           ++TYII   H D  +    +   WYT+    +N  SSL +    + +       ST+  +
Sbjct: 27  KKTYIIRVNHSDKPESFLTH-HDWYTS---QLNSESSLLYTYTTSFHGFSAYLDSTEADS 86

Query: 83  SLSKIRGFLAATPNELLQLHTTHSPQFLGLQKDHGLWNSSNLASDIIIGLLDTGIWPEHI 142
            LS     L    + L  LHTT +P+FLGL  + G+ +  + ++ +IIG+LDTG+WPE  
Sbjct: 87  LLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPESR 146

Query: 143 SFQDKGLPPVPSKWKGICQAGAKFSPSNCNKKLIGASAYIKGYE-AIVGRLNETGTFRSP 202
           SF D  +P +PSKWKG C++G+ F    CNKKLIGA ++ KG++ A  G  +      SP
Sbjct: 147 SFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVSP 206

Query: 203 RDSDGHGTHTASTAAGNIVNKASLNNQAMGVATGMSFTSRIAAYKVCWPLGCASADILAA 262
           RD DGHGTHT++TAAG+ V  AS    A G A GM+  +R+A YKVCW  GC  +DILAA
Sbjct: 207 RDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAA 266

Query: 263 MDSAVADGVDVLSLSLGGGAGSFYRDNIAIAAFGAIQNGVFVSCSAGNSGPSTSTVGNAA 322
           MD A+ DGVDVLSLSLGGG+  +YRD IAI AF A++ GVFVSCSAGNSGP+ ++V N A
Sbjct: 267 MDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVA 326

Query: 323 PWIMTVAASYTDRAFPTTVKLGNGQVFEGSSLY--VGKNINELPLVYNNTAGDGQETNFC 382
           PW+MTV A   DR FP    LGNG+   G SLY  VG     L LVYN   G+   +N C
Sbjct: 327 PWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNK--GNSSSSNLC 386

Query: 383 TTGSLDPSKVNGKIVLCERGLNSRTAKGEQVKLAGGAGMILINAQFEGEELFADPHVLPA 442
             GSLD S V GKIV+C+RG+N+R  KG  V+ AGG GMI+ N    GEEL AD H+LPA
Sbjct: 387 LPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPA 446

Query: 443 TTLGASAGIVILDYIASSESQAKASIAFEGTKYGSR-APRVAAFSSRGPSFVGPDVIKPD 502
             +G   G ++ +Y+  S+S+  A + F+GT    + +P VAAFSSRGP+ V P+++KPD
Sbjct: 447 IAVGKKTGDLLREYV-KSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPD 506

Query: 503 VTAPGVNILAAWPPIVSPSELESDKRRVLFNIISGTSMSCPHVSGLAALLKSAHKDWSPA 562
           V  PGVNILA W   + P+ L+ D RR  FNI+SGTSMSCPH+SGLA LLK+AH +WSP+
Sbjct: 507 VIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPS 566

Query: 563 AVKSALMTTAYVTDNKMSLISDVGDASGGPADPFAFGSGHVHPEKASDPGLVYDITPQDY 622
           A+KSALMTTAYV DN  + + D  D S   ++P+A GSGHV P+KA  PGLVYDI+ ++Y
Sbjct: 567 AIKSALMTTAYVLDNTNAPLHDAADNS--LSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 623 INYLCSLKYNSTQI-ALVSRGKLTCSSKRTFLQPGDLNYPSFSVFMKKKAKNVSITFKRT 682
           I +LCSL Y    I A+V R  + CS K  F  PG LNYPSFSV    K     + + R 
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVNCSKK--FSDPGQLNYPSFSVLFGGKR---VVRYTRE 686

Query: 683 VTNVGIPRSDYTVKINNPKGITVIVKPEKLSFGSLGEKLSYQVGFVALGGKQDLGEFSFG 742
           VTNVG   S Y V +N    + + VKP KLSF S+GEK  Y V FV+  G     +  FG
Sbjct: 687 VTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 743 SLVWVSGKYAVRSPIAVTW 754
           S+ W + ++ VRSP+A +W
Sbjct: 747 SITWSNPQHEVRSPVAFSW 751