HG10012628 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10012628 vs. NCBI nr
Match: XP_004134170.2 (putative E3 ubiquitin-protein ligase RF298 [Cucumis sativus] >KGN57044.1 hypothetical protein Csa_009823 [Cucumis sativus])

HSP 1 Score: 1600.9 bits (4144), Expect = 0.0e+00
Identity = 850/901 (94.34%), Postives = 874/901 (97.00%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSVGQTSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120
           AEKFEISSS+GQ+SGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDE QDA
Sbjct: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120

Query: 121 DWSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEA+LEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNALAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDM 240
           DN LAFLR GQEID SREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGS 300
           +V+LACAMDSDPCNALVCDGTSNESSSN+IPQLKAEVK+SEMNLPKPVKPISPISCAHGS
Sbjct: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300

Query: 301 QSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGS 360
           Q DGPAT+GVPSI+K KD LFSSGPLSEKELQNST D VEESFSVA NSQTSVSEEKI S
Sbjct: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTS 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYG KGSSRAGKL GLGGLMLDKKLKSVSGST+
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMP 480
           VNFKN SLKISKAMGIDVAQDNG+HNLST+DIPSSSLPFNLENINTVSPFSK N+PSSMP
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLPSSMP 480

Query: 481 APSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA NTSSA PTTDIDLSLSLPAKSNQP+VPFNCN ESSTSSFVEKP EK +G
Sbjct: 481 APSSPPALPALNTSSAPPTTDIDLSLSLPAKSNQPSVPFNCNPESSTSSFVEKPQEKFIG 540

Query: 541 QWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVLNL+PRV+ELQNQL EWT+WAN KVMQAARRLSKDKAELK LKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKALKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVK+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780
           EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE
Sbjct: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780

Query: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTGG 840
           ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRN+TDHKESWSPNVSESMKDLY+YSGTGG
Sbjct: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRYAR
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of HG10012628 vs. NCBI nr
Match: KAA0049418.1 (putative E3 ubiquitin-protein ligase RF298 [Cucumis melo var. makuwa] >TYK16096.1 putative E3 ubiquitin-protein ligase RF298 [Cucumis melo var. makuwa])

HSP 1 Score: 1600.1 bits (4142), Expect = 0.0e+00
Identity = 849/901 (94.23%), Postives = 873/901 (96.89%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSVGQTSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120
           AEKFEISSS+GQ+ GCDLC ISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA
Sbjct: 61  AEKFEISSSMGQSGGCDLCGISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEA+LEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNALAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDM 240
           DN LAFLRSGQEID SREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGS 300
           NVSLACAMDS+PCNALVCDGTSNES  N++PQLKAEVK+SEMNLPKPVKPISPIS AHGS
Sbjct: 241 NVSLACAMDSNPCNALVCDGTSNESLPNTVPQLKAEVKSSEMNLPKPVKPISPISSAHGS 300

Query: 301 QSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGS 360
           QSDGPAT+GVPSI+K KD LFSSGPLSEKELQNST D VEESFSVA NSQTSVSEEKI S
Sbjct: 301 QSDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTS 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKL GLGGLMLDKKLKSVSGST+
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMP 480
           VNFKN SLKISKAMGIDVAQDNG+HNLST+DIPSSSLPFNLENINTVS FSK N+PSSMP
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSLFSKTNLPSSMP 480

Query: 481 APSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA NTSSA PTTDIDLSLSLP KSNQP+VPFNCN ESSTSSFVEKP+EK +G
Sbjct: 481 APSSPPALPAMNTSSAPPTTDIDLSLSLPTKSNQPSVPFNCNPESSTSSFVEKPHEKFVG 540

Query: 541 QWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVLNL+PRV+ELQNQL EWT+WAN KVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVK+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780
           EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE
Sbjct: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780

Query: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTGG 840
           ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLY+YSGTGG
Sbjct: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYKYSGTGG 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRYAR
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of HG10012628 vs. NCBI nr
Match: XP_008438753.1 (PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Cucumis melo])

HSP 1 Score: 1598.9 bits (4139), Expect = 0.0e+00
Identity = 849/901 (94.23%), Postives = 872/901 (96.78%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHG SSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGSSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSVGQTSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120
           AEKFEISSS+GQ+ GCDLC ISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA
Sbjct: 61  AEKFEISSSMGQSGGCDLCGISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEA+LEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNALAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDM 240
           DN LAFLRSGQEID SREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGS 300
           NVSLACAMDSDPCNALVCDGTSNES  N++PQLKAEVK+SEMNLPKPVKPISPIS AHGS
Sbjct: 241 NVSLACAMDSDPCNALVCDGTSNESLPNTVPQLKAEVKSSEMNLPKPVKPISPISSAHGS 300

Query: 301 QSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGS 360
           QSDGPAT+GVPSI+K KD LFSSGPLSEKELQNST D VEESFSVA NSQTSVSEEKI S
Sbjct: 301 QSDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTS 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKL GLGGLMLDKKLKSVSGST+
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMP 480
           VNFKN SLKISKAMGIDVAQDNG+HNLST+DIPSSSLPFNLENINTVS FSK N+PSSMP
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSLFSKTNLPSSMP 480

Query: 481 APSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA NTSSA PTTDIDLSLSLP KSNQP+VPFNCN ESSTSSFVEKP+EK +G
Sbjct: 481 APSSPPALPAMNTSSAPPTTDIDLSLSLPTKSNQPSVPFNCNPESSTSSFVEKPHEKFVG 540

Query: 541 QWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVLNL+PRV+ELQNQL EWT+WAN KVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVK+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780
           EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE
Sbjct: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780

Query: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTGG 840
           ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLY+YSGTGG
Sbjct: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYKYSGTGG 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRYAR
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of HG10012628 vs. NCBI nr
Match: XP_038893729.1 (LOW QUALITY PROTEIN: putative E3 ubiquitin-protein ligase RF298 [Benincasa hispida])

HSP 1 Score: 1518.1 bits (3929), Expect = 0.0e+00
Identity = 819/902 (90.80%), Postives = 837/902 (92.79%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPST NHGPSSMTVQEKGSRNKRKYRADPPLGDLNKI SSSQDECPSYEFS
Sbjct: 1   MASMVAKPSCPSTINHGPSSMTVQEKGSRNKRKYRADPPLGDLNKIISSSQDECPSYEFS 60

Query: 61  AEKFEISSSVGQTSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120
           AEKFEISSSVGQT+GCDLCSISQEFSAGLKLDLGLSNGGSS+VGINWPRGELEVDEYQDA
Sbjct: 61  AEKFEISSSVGQTTGCDLCSISQEFSAGLKLDLGLSNGGSSEVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDL EA+LEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV
Sbjct: 121 DWSDLIEAQLEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180

Query: 181 DNALAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDM 240
           DN LAFLRSGQEID SREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDM 240

Query: 241 NVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGS 300
           NVSLACAMDSDPCNALVCDGTSNESSSNSIPQLK +VK+SEMNLPKPVKPISPISCAHGS
Sbjct: 241 NVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKEDVKSSEMNLPKPVKPISPISCAHGS 300

Query: 301 QSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGS 360
           QSDG ATLGVPSITK KD LFSSGP SEKE+QNSTSD VEESFSVAGNSQ SVSEEKIGS
Sbjct: 301 QSDGSATLGVPSITKLKDPLFSSGPFSEKEVQNSTSDIVEESFSVAGNSQASVSEEKIGS 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTS 420
           SRKV SN+TKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVS ST+
Sbjct: 361 SRKVQSNVTKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSASTA 420

Query: 421 VNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMP 480
           VNFKN SLKISKAMGIDVAQDNGNHNLST+DIPSSSL FNLENINT+SPFSK NV SSMP
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGNHNLSTLDIPSSSLSFNLENINTISPFSKTNVSSSMP 480

Query: 481 APSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPATNTSS LPTTDIDLSLSLPAKSNQP+   NCNAESSTSS  E    K LG
Sbjct: 481 APSSPPALPATNTSSVLPTTDIDLSLSLPAKSNQPSXSINCNAESSTSSCGET--MKVLG 540

Query: 541 QWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEE 600
           Q FPRDKKDEMVLN   +   +    HEWTEW NHKVMQAARRLSKDKAELKTLKQ KEE
Sbjct: 541 QXFPRDKKDEMVLNSSAKSSGITKSAHEWTEWXNHKVMQAARRLSKDKAELKTLKQXKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKA-SGQVELANSAVRRLEVENAALRQDMEVAKL 660
           VERLKKEKQTLEENTMKKLSEME  +CKA  GQVELA + +RRLEVENAALRQDMEVAKL
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEQCICKAKDGQVELAXTLLRRLEVENAALRQDMEVAKL 660

Query: 661 RATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQ 720
           RATESAASYQEV +  KKTLMKVQSWEKQKML QEE  +EKRKVK    E EQARDLQ  
Sbjct: 661 RATESAASYQEVLRXGKKTLMKVQSWEKQKMLXQEETHSEKRKVKPTNTEFEQARDLQSN 720

Query: 721 LEGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEK 780
            EGRWKLEERAKDELLV AASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEK
Sbjct: 721 XEGRWKLEERAKDELLVLAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEK 780

Query: 781 EISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTG 840
           EISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSG G
Sbjct: 781 EISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGMG 840

Query: 841 GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYA 900
           GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRYA
Sbjct: 841 GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYA 900

Query: 901 RS 902
           RS
Sbjct: 901 RS 900

BLAST of HG10012628 vs. NCBI nr
Match: XP_022137773.1 (putative E3 ubiquitin-protein ligase RF298 isoform X1 [Momordica charantia])

HSP 1 Score: 1498.0 bits (3877), Expect = 0.0e+00
Identity = 802/902 (88.91%), Postives = 843/902 (93.46%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNH PSSMTVQEKGSRNKRKYRADPPLGDLNKIT SSQDECPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHVPSSMTVQEKGSRNKRKYRADPPLGDLNKITMSSQDECPSYEFS 60

Query: 61  AEKFEISSSVGQTSGCDLCSISQEFSAGLKLDLGLSNG-GSSDVGINWPRGELEVDEYQD 120
           AEKFEISS++GQTS CD+CSISQE+SA LKLDLGLSNG GSSDVG+NWPR EL+VDE +D
Sbjct: 61  AEKFEISSNIGQTSACDICSISQEYSAALKLDLGLSNGLGSSDVGLNWPREELDVDEVED 120

Query: 121 ADWSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNI 180
           ADWSDLTEA+LEELVL+NLDTIFKSA KKIVASGY+EEVAIKAVSR+GICFG KDTVSNI
Sbjct: 121 ADWSDLTEAQLEELVLSNLDTIFKSATKKIVASGYSEEVAIKAVSRAGICFGSKDTVSNI 180

Query: 181 VDNALAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISD 240
           VDN LAFLRSGQEID SREH FEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLI D
Sbjct: 181 VDNTLAFLRSGQEIDHSREHCFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD 240

Query: 241 MNVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHG 300
           MNV+ ACAMDSDP NA  CDG SNESSSNSIPQ+KAE K SEMNLPKPVKPISPISCAH 
Sbjct: 241 MNVTHACAMDSDPFNAFFCDGASNESSSNSIPQIKAEAKISEMNLPKPVKPISPISCAHS 300

Query: 301 SQSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIG 360
           SQS+ PATLGVP++ K+KD +F SGP+S+KE Q+S SD  EESF+VAGNSQTSVSEEKIG
Sbjct: 301 SQSEEPATLGVPNLPKTKDPVFLSGPVSDKEFQSSASDVAEESFNVAGNSQTSVSEEKIG 360

Query: 361 SSRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGST 420
           SSRK HSNITKREYMLRQKSLHV+KNFRTYGPKGSSR GKL GLGGLMLDKK+KSVS ST
Sbjct: 361 SSRKFHSNITKREYMLRQKSLHVEKNFRTYGPKGSSRGGKLTGLGGLMLDKKIKSVSSST 420

Query: 421 SVNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSM 480
           SVNFKN S KISKAMGIDV QD GNH+LSTIDIP+SSL F LENI+T+SPFSK N  SSM
Sbjct: 421 SVNFKNAS-KISKAMGIDVIQDIGNHSLSTIDIPASSLAF-LENISTISPFSKANTLSSM 480

Query: 481 PAPSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSL 540
           PAPSSPPALP  NTSSAL T+DIDLSLSLP KSNQP++P + NAESS+SSFVEK YEKSL
Sbjct: 481 PAPSSPPALPEANTSSALSTSDIDLSLSLPTKSNQPSMPISYNAESSSSSFVEKSYEKSL 540

Query: 541 GQWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKE 600
           GQWFPRDKKDEMVL LVPR RELQNQL EWTEWAN KVMQAARRLSKDKAELK LKQEKE
Sbjct: 541 GQWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKALKQEKE 600

Query: 601 EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKL 660
           EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQ+MEVAKL
Sbjct: 601 EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKL 660

Query: 661 RATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQ 720
           RATESAASYQEVS+REKKTLMKVQSWEKQK LFQEEHTAEKRK+KQLIQEL+QARDLQEQ
Sbjct: 661 RATESAASYQEVSRREKKTLMKVQSWEKQKTLFQEEHTAEKRKLKQLIQELDQARDLQEQ 720

Query: 721 LEGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEK 780
           LEGRWKLEERAKDE+L+QAASLRKEREQIE SVK KEDTIKLKAENNL+KYKDDIQKLEK
Sbjct: 721 LEGRWKLEERAKDEVLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKYKDDIQKLEK 780

Query: 781 EISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTG 840
           EIS LRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESW+PNVSESMKDLYEYSGTG
Sbjct: 781 EISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMKDLYEYSGTG 840

Query: 841 GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYA 900
           GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRYA
Sbjct: 841 GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYA 900

Query: 901 RS 902
           RS
Sbjct: 901 RS 900

BLAST of HG10012628 vs. ExPASy Swiss-Prot
Match: Q0WPJ7 (Putative E3 ubiquitin-protein ligase RF298 OS=Arabidopsis thaliana OX=3702 GN=RF298 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.4e-130
Identity = 350/890 (39.33%), Postives = 503/890 (56.52%), Query Frame = 0

Query: 24  QEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSVGQTSGCDLCSISQ 83
           Q+KG +NKRK      L D +   ++S  E P YE  + K          S   LC    
Sbjct: 29  QDKGRKNKRK------LADPSPQNAASLTEFPRYELHSFK----------SQSPLCE--- 88

Query: 84  EFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDADWSDLTEAELEELVLNNLDTIFK 143
                                 N   G+L+ +E     W D     LE L+ +NL T+F+
Sbjct: 89  ----------------------NDSNGQLKAEESDSVGWDDPFACHLEGLLSSNLLTLFR 148

Query: 144 SAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNALAFLRSGQEIDQSREHYFED 203
           SA+ +I+  GY+E+V +KA+S S    G  D VSNIV++ L+FL+SG+++  SR++ FED
Sbjct: 149 SAMNQIMDCGYSEDVVLKAISSSRFYCGGTDLVSNIVNDTLSFLKSGKKVAGSRDYVFED 208

Query: 204 LQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDMNVSLACAMDSDPCNALVCDGTSN 263
           LQQL  Y L E + ++REVRP  ST +AMW LLI D+NV  A  +D+D    L     SN
Sbjct: 209 LQQLVAYSLVEKISLVREVRPSLSTDEAMWRLLICDLNVLKAFEVDAD---GLEGSSVSN 268

Query: 264 ESSSNSIPQLKAEVKTSEMNLPKPV---KPISPISCAHGSQSDGPATLGVPSITKSKDLL 323
            S S+  P        +E N PK      P +P+S     QS+        ++  SK+  
Sbjct: 269 ASKSSESP-------VAECNPPKSSDADNPKAPVSNTQSKQSEPVKFGNFANVNNSKN-P 328

Query: 324 FSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREY-MLRQKS 383
            +SG    KE+ + ++ + E + S    S TSVS+EK+ S RK     TK+E  MLRQKS
Sbjct: 329 HASGATPGKEVFSVSTASGEGTKSA---SLTSVSDEKLVSCRK---GRTKKEMAMLRQKS 388

Query: 384 LHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTSVNFKNTSLKI-SKAMGIDV 443
             V+K  RTY   G  +  K    GG +++K+ KS S   S   +N+S KI ++ M I +
Sbjct: 389 C-VEK-IRTYSKGGGYKTAK---FGGFLVEKRGKSASDLLSAQARNSSSKITTEVMKIPL 448

Query: 444 AQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMPAPSSPPALPATNTSSALP 503
           A+ +   + ST                                 S  PAL      +ALP
Sbjct: 449 AESSSTLSNST--------------------------------KSDSPALDVKEHVTALP 508

Query: 504 TTDIDLSLSLPAKS-NQPTVPFNCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMVLNLVP 563
             +    ++   KS ++P    + + + +   +   PY+ +LG + PR+K+DE++L LVP
Sbjct: 509 ANNAPAPVASEKKSGSEPEEKPSVSTKPAPDYYAAIPYDATLGIYIPRNKRDELILKLVP 568

Query: 564 RVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLEENTMK 623
           R+++LQ +L +WT+WAN KV QA  RL KD+ ELK L++EKEE E  +KEKQ LEENT+K
Sbjct: 569 RMKDLQKELQDWTDWANQKVKQATVRLLKDQPELKALRKEKEEAEEFRKEKQLLEENTIK 628

Query: 624 KLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLRATESAASYQEVSKREKK 683
           + SEME AL  A+ Q+E  N+ +RRLE+E + L+++ E A +RA+ESA S +E  +R ++
Sbjct: 629 RRSEMELALNNATNQLERTNNTIRRLELEQSLLKREREAANIRASESAESCREAKERVQR 688

Query: 684 TLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQLEGRWKLEERAKDELLVQ 743
            L   QSWE QK L QEE  +++ KV  L QE+ +A+  Q Q+E  WK E+ A  +L  Q
Sbjct: 689 LLKNSQSWEGQKNLLQEELKSQRDKVAGLQQEVAKAKTRQNQIEATWKQEKSATGKLTAQ 748

Query: 744 AASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKEISVLRLKTDSSRIAALK 803
           AA+L+KER ++E+  K +E+ IK KAEN++  Y ++I++L+ EIS L+LK+DS +IAALK
Sbjct: 749 AAALKKERGKLEELGKAEEERIKTKAENDVKYYIENIKRLDTEISKLKLKSDSLKIAALK 808

Query: 804 RGIDG------SYASRLTDTR-NSTDHKESWSPNVSESMKDLYEYSGTGGVKRERECVMC 863
           +GIDG      S  +  T+T+ NS    + W  N     K          +KRERECVMC
Sbjct: 809 KGIDGNNDGNKSGMNHTTNTKANSMASAKVWENNQGAESK----------IKRERECVMC 813

Query: 864 LSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYAR 901
           LSEEMSV+FLPCAHQV+C+ CN+LHEK+ M+DCPSCR+ IQRRI  R+AR
Sbjct: 869 LSEEMSVIFLPCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 813

BLAST of HG10012628 vs. ExPASy Swiss-Prot
Match: Q9ZVT8 (Putative E3 ubiquitin-protein ligase RF4 OS=Arabidopsis thaliana OX=3702 GN=RF4 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 4.4e-124
Identity = 339/889 (38.13%), Postives = 498/889 (56.02%), Query Frame = 0

Query: 20  SMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSVGQTSGCDLC 79
           +++ QEKG +NKRK      L D ++  +SS  E P YE  +                  
Sbjct: 24  TVSPQEKGRKNKRK------LADPSQPNASSLTEFPPYELPS------------------ 83

Query: 80  SISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDADWSDLTEAELEELVLNNLD 139
                    LK    LS  GS    +     +L+V+  +  +W D     LEEL+ +NL 
Sbjct: 84  ---------LKPQNHLSGNGS----VGEVSNQLQVEVSESVEWDDPFACHLEELLSSNLL 143

Query: 140 TIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNALAFLRSGQEIDQSREH 199
           T+F   +K+++  GYT++  +KAVSR  +  G  + +SNIV+N L+ L++G E   S ++
Sbjct: 144 TLFLDTMKQLIDLGYTDDEVLKAVSRCRLYCGGNNLLSNIVNNTLSALKTGDEGAGSGDY 203

Query: 200 YFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDMNVSLACAMDSDPCNALVCD 259
            FEDLQQL  Y L E++ +++EVRP  ST +AMW LL+ D+NV  A   + D    LV  
Sbjct: 204 VFEDLQQLVSYTLVEMISLIKEVRPSLSTVEAMWRLLMCDLNVLQAFEAEGD---GLV-- 263

Query: 260 GTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGSQSDGPATLGVPSITKSKDL 319
            +S  S S S+       K+S+ + PKP     P S    ++++ P   G    T +   
Sbjct: 264 SSSKLSDSESLGAESNPPKSSDPDNPKP-----PQSDPQSNRNE-PLKFGNFPNTPNSKK 323

Query: 320 LFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREY-MLRQK 379
             SSG    KE+ + ++ + +    +   S T VS+EK+ S RK     TK+E  MLRQK
Sbjct: 324 TQSSGTTPGKEVCSGSTVSCQ---GMRSTSFTLVSDEKLVSCRK---GRTKKEIAMLRQK 383

Query: 380 SLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTSVNFKNTSLKISKAMGIDV 439
           S  V+K  RTY      +A K A +G  +L+K++KS   S+    +N+S KI+  +G+ V
Sbjct: 384 SC-VEK-IRTYSKGSGYKAAKFASVGSFLLEKRVKS---SSEFVPRNSSSKITAEIGVKV 443

Query: 440 --AQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMPAPSSP----PALPATN 499
             A+D+G        + S  +  + +   T  P   +   S     S      P+     
Sbjct: 444 SLAEDSGCFVRKNSKLDSPVVVVDAKGYITALPARSVKSASKKKTGSESVTLIPSASEKK 503

Query: 500 TSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMV 559
           + S++P+T      S     ++     + +A+ +   +   PY+ +LG + PRDKKDE++
Sbjct: 504 SDSSIPST------SEKKSGSESEEKASVSAKLAPDYYAGIPYDAALGIYVPRDKKDELI 563

Query: 560 LNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLE 619
           L LVPRV +LQN+L  WT+WAN KV +A  RL KD+ ELK L++E+EE E+ KKEKQ LE
Sbjct: 564 LKLVPRVNDLQNELQVWTDWANQKVKEATGRLLKDQPELKALRKEREEAEQYKKEKQLLE 623

Query: 620 ENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLRATESAASYQEVS 679
           ENT K+LSEM+ AL  A+ Q+E A +   RLE+E + L+++ME AK++A ESA S++E  
Sbjct: 624 ENTRKRLSEMDFALKNATSQLEKAFNTAHRLELEQSILKKEMEAAKIKAVESAESFREAK 683

Query: 680 KREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQLEGRWKLEERAKD 739
           +R +++L  + SWE QK++ QEE   ++ KV  L +E+ +A++ Q Q+E   K E  AK 
Sbjct: 684 ERGERSLKDIHSWEGQKIMLQEELKGQREKVTVLQKEVTKAKNRQNQIEAALKQERTAKG 743

Query: 740 ELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKEISVLRLKTDSSR 799
           +L  QA+ +RKE +++E   KV+E+ IK KAE ++  Y D+I++LE+EIS L+LK+D SR
Sbjct: 744 KLSAQASLIRKETKELEALGKVEEERIKGKAETDVKYYIDNIKRLEREISELKLKSDYSR 803

Query: 800 IAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTGGVKRERECVMCLS 859
           I ALK+G                          SES     E  G   VKRERECVMCLS
Sbjct: 804 IIALKKG-------------------------SSESKATKRESLGMPKVKRERECVMCLS 822

Query: 860 EEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYARS 902
           EEMSV+FLPCAHQV+C  CN+LHEK+GM DCPSCR  I RRI  R+ARS
Sbjct: 864 EEMSVIFLPCAHQVLCFKCNQLHEKEGMMDCPSCRGTIHRRIQARFARS 822

BLAST of HG10012628 vs. ExPASy Swiss-Prot
Match: Q8RX22 (MND1-interacting protein 1 OS=Arabidopsis thaliana OX=3702 GN=MIP1 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 3.0e-64
Identity = 241/845 (28.52%), Postives = 397/845 (46.98%), Query Frame = 0

Query: 80  SISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDADWSDLTEAELEELVLNNLD 139
           ++SQ     L    GL++ GS  V +N        +  ++  W+  TE  LEE++L +L+
Sbjct: 34  AMSQSIVENLVYHPGLTDSGS--VNLN-----SVTENPEENFWAYCTEEHLEEILLKHLE 93

Query: 140 TIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNALAFLRSG-------QE 199
            ++  A+ K++  GY E VA+KAV  +G C+G  D ++NIV+N+L++L SG         
Sbjct: 94  FLYNQAVSKLLELGYEERVALKAVLSNGHCYGELDVLTNIVNNSLSYLNSGGGGGGSNGN 153

Query: 200 IDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDMNVSLACAMDSDP 259
            +   E  F DL+ LE+Y LA ++ +L++V+P  S GDAMWCLL+S+++V  A  +D   
Sbjct: 154 GEDRTETGFTDLRDLEEYSLAGMIYLLQQVKPNLSKGDAMWCLLMSELHVGRASTLD--- 213

Query: 260 CNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGSQSDGPATLGVPS 319
                                                P +  SC                
Sbjct: 214 ------------------------------------VPTNRSSCC--------------- 273

Query: 320 ITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGSSRKVHSNITKRE 379
                           KE  N        +  +AG    ++                   
Sbjct: 274 ---------------TKEDSNVEDVGTGGTLDIAGFMAPALC------------------ 333

Query: 380 YMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTSVNFKNTSLKISK 439
                         R +G  G    G                 SG+   + K   LK+ +
Sbjct: 334 --------------RFHGGWGFGNGGG-------------PEFSGN-GFSMKGAELKLQR 393

Query: 440 AMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMPAPSSPPALPATN 499
                            ID P     FNL      SP  K  +  ++ A ++     A+ 
Sbjct: 394 ----------------EIDCPKR---FNL------SPSMKSLLKRNVAAFAA--GYRASM 453

Query: 500 TSSALPTTDIDLSLSLPAKSNQPTVPFNC-------NAESSTSSFVEKPYEKSLGQWFPR 559
               + ++D   ++      N P +  +C        +E S S+ +EK  + +L      
Sbjct: 454 KQKQIQSSD---TIGDSKACNDPAIVKSCGQQPRKSGSEESVSTVLEKFRDLNLDDNLES 513

Query: 560 ---DKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEEVE 619
              D KD ++++L+ +V++ + ++ E  EWA    MQAA+++S++ AELKTL  E+E ++
Sbjct: 514 VGVDDKDCVIVDLLHQVKDFEKKVKERKEWAQKNAMQAAQKVSEELAELKTLSSEREGIQ 573

Query: 620 RLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLRAT 679
            LKK KQ +EE+T K+ ++ E  L KA  Q + AN  VR+LE +NA +R + E +KL A+
Sbjct: 574 LLKKGKQAVEESTAKRFTDKEIELRKACSQNDRANVIVRKLENQNAEIRAEREGSKLSAS 633

Query: 680 ESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQLEG 739
           ES  +  E SK+EKK L K+ +WEKQ +  Q+E TAEK K+K L + L Q  + ++++E 
Sbjct: 634 ESLKACMEASKKEKKCLKKLVAWEKQILKLQDEITAEKEKIKALYKTLAQITEYEKEIEA 693

Query: 740 RWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKEIS 799
           +W+ E++AK+E L Q    ++ +E  E   K K +T++LK E +  ++KDD Q+LE+E+ 
Sbjct: 694 KWRQEQKAKEEALAQMEEEQRSKEAAEGHNKRKLETLRLKIELDFQRHKDDHQRLEQELG 711

Query: 800 VLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGT--GG 859
            L+  +DS          D S+ S      N+   K+S   N+++ ++++ +  G+    
Sbjct: 754 RLKASSDS----------DSSHIS-----NNAWKPKKSQGENIAKLLEEIDKLEGSYDNE 711

Query: 860 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNE---LHEKQGMK-DCPSCRSLIQRRIPV 902
              +REC++C+ +E+SVVFLPCAHQVVC +C++        G K  CP CR L+Q+RI +
Sbjct: 814 ANYDRECIICMKDEVSVVFLPCAHQVVCGSCSDSFFASNNGGSKVTCPCCRGLVQQRIRI 711

BLAST of HG10012628 vs. ExPASy TrEMBL
Match: A0A0A0L7S3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G150240 PE=4 SV=1)

HSP 1 Score: 1600.9 bits (4144), Expect = 0.0e+00
Identity = 850/901 (94.34%), Postives = 874/901 (97.00%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSVGQTSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120
           AEKFEISSS+GQ+SGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDE QDA
Sbjct: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120

Query: 121 DWSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEA+LEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNALAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDM 240
           DN LAFLR GQEID SREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGS 300
           +V+LACAMDSDPCNALVCDGTSNESSSN+IPQLKAEVK+SEMNLPKPVKPISPISCAHGS
Sbjct: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300

Query: 301 QSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGS 360
           Q DGPAT+GVPSI+K KD LFSSGPLSEKELQNST D VEESFSVA NSQTSVSEEKI S
Sbjct: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTS 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYG KGSSRAGKL GLGGLMLDKKLKSVSGST+
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMP 480
           VNFKN SLKISKAMGIDVAQDNG+HNLST+DIPSSSLPFNLENINTVSPFSK N+PSSMP
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLPSSMP 480

Query: 481 APSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA NTSSA PTTDIDLSLSLPAKSNQP+VPFNCN ESSTSSFVEKP EK +G
Sbjct: 481 APSSPPALPALNTSSAPPTTDIDLSLSLPAKSNQPSVPFNCNPESSTSSFVEKPQEKFIG 540

Query: 541 QWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVLNL+PRV+ELQNQL EWT+WAN KVMQAARRLSKDKAELK LKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKALKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVK+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780
           EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE
Sbjct: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780

Query: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTGG 840
           ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRN+TDHKESWSPNVSESMKDLY+YSGTGG
Sbjct: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMKDLYKYSGTGG 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRYAR
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of HG10012628 vs. ExPASy TrEMBL
Match: A0A5D3D0B8 (Putative E3 ubiquitin-protein ligase RF298 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00180 PE=4 SV=1)

HSP 1 Score: 1600.1 bits (4142), Expect = 0.0e+00
Identity = 849/901 (94.23%), Postives = 873/901 (96.89%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSVGQTSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120
           AEKFEISSS+GQ+ GCDLC ISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA
Sbjct: 61  AEKFEISSSMGQSGGCDLCGISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEA+LEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNALAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDM 240
           DN LAFLRSGQEID SREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGS 300
           NVSLACAMDS+PCNALVCDGTSNES  N++PQLKAEVK+SEMNLPKPVKPISPIS AHGS
Sbjct: 241 NVSLACAMDSNPCNALVCDGTSNESLPNTVPQLKAEVKSSEMNLPKPVKPISPISSAHGS 300

Query: 301 QSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGS 360
           QSDGPAT+GVPSI+K KD LFSSGPLSEKELQNST D VEESFSVA NSQTSVSEEKI S
Sbjct: 301 QSDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTS 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKL GLGGLMLDKKLKSVSGST+
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMP 480
           VNFKN SLKISKAMGIDVAQDNG+HNLST+DIPSSSLPFNLENINTVS FSK N+PSSMP
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSLFSKTNLPSSMP 480

Query: 481 APSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA NTSSA PTTDIDLSLSLP KSNQP+VPFNCN ESSTSSFVEKP+EK +G
Sbjct: 481 APSSPPALPAMNTSSAPPTTDIDLSLSLPTKSNQPSVPFNCNPESSTSSFVEKPHEKFVG 540

Query: 541 QWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVLNL+PRV+ELQNQL EWT+WAN KVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVK+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780
           EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE
Sbjct: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780

Query: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTGG 840
           ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLY+YSGTGG
Sbjct: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYKYSGTGG 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRYAR
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of HG10012628 vs. ExPASy TrEMBL
Match: A0A1S3AX62 (putative E3 ubiquitin-protein ligase RF298 OS=Cucumis melo OX=3656 GN=LOC103483769 PE=4 SV=1)

HSP 1 Score: 1598.9 bits (4139), Expect = 0.0e+00
Identity = 849/901 (94.23%), Postives = 872/901 (96.78%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHG SSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGSSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSVGQTSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120
           AEKFEISSS+GQ+ GCDLC ISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA
Sbjct: 61  AEKFEISSSMGQSGGCDLCGISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEA+LEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNALAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDM 240
           DN LAFLRSGQEID SREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLISDM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGS 300
           NVSLACAMDSDPCNALVCDGTSNES  N++PQLKAEVK+SEMNLPKPVKPISPIS AHGS
Sbjct: 241 NVSLACAMDSDPCNALVCDGTSNESLPNTVPQLKAEVKSSEMNLPKPVKPISPISSAHGS 300

Query: 301 QSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGS 360
           QSDGPAT+GVPSI+K KD LFSSGPLSEKELQNST D VEESFSVA NSQTSVSEEKI S
Sbjct: 301 QSDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTS 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKL GLGGLMLDKKLKSVSGST+
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMP 480
           VNFKN SLKISKAMGIDVAQDNG+HNLST+DIPSSSLPFNLENINTVS FSK N+PSSMP
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSLFSKTNLPSSMP 480

Query: 481 APSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA NTSSA PTTDIDLSLSLP KSNQP+VPFNCN ESSTSSFVEKP+EK +G
Sbjct: 481 APSSPPALPAMNTSSAPPTTDIDLSLSLPTKSNQPSVPFNCNPESSTSSFVEKPHEKFVG 540

Query: 541 QWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVLNL+PRV+ELQNQL EWT+WAN KVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVK+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780
           EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE
Sbjct: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780

Query: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTGG 840
           ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLY+YSGTGG
Sbjct: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYKYSGTGG 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRYAR
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of HG10012628 vs. ExPASy TrEMBL
Match: A0A6J1CBB5 (putative E3 ubiquitin-protein ligase RF298 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009134 PE=4 SV=1)

HSP 1 Score: 1498.0 bits (3877), Expect = 0.0e+00
Identity = 802/902 (88.91%), Postives = 843/902 (93.46%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNH PSSMTVQEKGSRNKRKYRADPPLGDLNKIT SSQDECPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHVPSSMTVQEKGSRNKRKYRADPPLGDLNKITMSSQDECPSYEFS 60

Query: 61  AEKFEISSSVGQTSGCDLCSISQEFSAGLKLDLGLSNG-GSSDVGINWPRGELEVDEYQD 120
           AEKFEISS++GQTS CD+CSISQE+SA LKLDLGLSNG GSSDVG+NWPR EL+VDE +D
Sbjct: 61  AEKFEISSNIGQTSACDICSISQEYSAALKLDLGLSNGLGSSDVGLNWPREELDVDEVED 120

Query: 121 ADWSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNI 180
           ADWSDLTEA+LEELVL+NLDTIFKSA KKIVASGY+EEVAIKAVSR+GICFG KDTVSNI
Sbjct: 121 ADWSDLTEAQLEELVLSNLDTIFKSATKKIVASGYSEEVAIKAVSRAGICFGSKDTVSNI 180

Query: 181 VDNALAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISD 240
           VDN LAFLRSGQEID SREH FEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLI D
Sbjct: 181 VDNTLAFLRSGQEIDHSREHCFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD 240

Query: 241 MNVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHG 300
           MNV+ ACAMDSDP NA  CDG SNESSSNSIPQ+KAE K SEMNLPKPVKPISPISCAH 
Sbjct: 241 MNVTHACAMDSDPFNAFFCDGASNESSSNSIPQIKAEAKISEMNLPKPVKPISPISCAHS 300

Query: 301 SQSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIG 360
           SQS+ PATLGVP++ K+KD +F SGP+S+KE Q+S SD  EESF+VAGNSQTSVSEEKIG
Sbjct: 301 SQSEEPATLGVPNLPKTKDPVFLSGPVSDKEFQSSASDVAEESFNVAGNSQTSVSEEKIG 360

Query: 361 SSRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGST 420
           SSRK HSNITKREYMLRQKSLHV+KNFRTYGPKGSSR GKL GLGGLMLDKK+KSVS ST
Sbjct: 361 SSRKFHSNITKREYMLRQKSLHVEKNFRTYGPKGSSRGGKLTGLGGLMLDKKIKSVSSST 420

Query: 421 SVNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSM 480
           SVNFKN S KISKAMGIDV QD GNH+LSTIDIP+SSL F LENI+T+SPFSK N  SSM
Sbjct: 421 SVNFKNAS-KISKAMGIDVIQDIGNHSLSTIDIPASSLAF-LENISTISPFSKANTLSSM 480

Query: 481 PAPSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSL 540
           PAPSSPPALP  NTSSAL T+DIDLSLSLP KSNQP++P + NAESS+SSFVEK YEKSL
Sbjct: 481 PAPSSPPALPEANTSSALSTSDIDLSLSLPTKSNQPSMPISYNAESSSSSFVEKSYEKSL 540

Query: 541 GQWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKE 600
           GQWFPRDKKDEMVL LVPR RELQNQL EWTEWAN KVMQAARRLSKDKAELK LKQEKE
Sbjct: 541 GQWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKALKQEKE 600

Query: 601 EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKL 660
           EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQ+MEVAKL
Sbjct: 601 EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKL 660

Query: 661 RATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQ 720
           RATESAASYQEVS+REKKTLMKVQSWEKQK LFQEEHTAEKRK+KQLIQEL+QARDLQEQ
Sbjct: 661 RATESAASYQEVSRREKKTLMKVQSWEKQKTLFQEEHTAEKRKLKQLIQELDQARDLQEQ 720

Query: 721 LEGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEK 780
           LEGRWKLEERAKDE+L+QAASLRKEREQIE SVK KEDTIKLKAENNL+KYKDDIQKLEK
Sbjct: 721 LEGRWKLEERAKDEVLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKYKDDIQKLEK 780

Query: 781 EISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTG 840
           EIS LRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESW+PNVSESMKDLYEYSGTG
Sbjct: 781 EISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMKDLYEYSGTG 840

Query: 841 GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYA 900
           GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRYA
Sbjct: 841 GVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYA 900

Query: 901 RS 902
           RS
Sbjct: 901 RS 900

BLAST of HG10012628 vs. ExPASy TrEMBL
Match: A0A6J1GLE6 (putative E3 ubiquitin-protein ligase RF298 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455308 PE=4 SV=1)

HSP 1 Score: 1491.5 bits (3860), Expect = 0.0e+00
Identity = 787/901 (87.35%), Postives = 835/901 (92.67%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCP+TSNHGPSS+ VQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSY+FS
Sbjct: 1   MASMVAKPSCPATSNHGPSSVLVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYDFS 60

Query: 61  AEKFEISSSVGQTSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120
           AEKFEISSS+GQ   CDLCSISQEFSAGLKLDLGLSNGGS DVGI+WPRGELEVDE+QD 
Sbjct: 61  AEKFEISSSLGQIGACDLCSISQEFSAGLKLDLGLSNGGSPDVGIDWPRGELEVDEFQDV 120

Query: 121 DWSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEA+L EL+L NLDTIFK+AIKK+VASG+TEEVAIKA+SRSGICFGCKD +SN+V
Sbjct: 121 DWSDLTEAQLVELILCNLDTIFKAAIKKVVASGFTEEVAIKAISRSGICFGCKDIMSNVV 180

Query: 181 DNALAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDM 240
           DN LAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRP+FSTGDAMWCLL+SDM
Sbjct: 181 DNTLAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPYFSTGDAMWCLLVSDM 240

Query: 241 NVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGS 300
           +VS AC MDSDP NA VCDGTSNE S+NS PQLKAE K+SE+N PKP+KPISPISCAH S
Sbjct: 241 SVSHACTMDSDPSNAGVCDGTSNEGSTNSTPQLKAEAKSSELNFPKPLKPISPISCAHSS 300

Query: 301 QSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGS 360
           QSDGPATLGVP++TK KD LFSS  +S+KELQNS SD   ESF+VAGN QTSV+EEKIGS
Sbjct: 301 QSDGPATLGVPNLTKPKDPLFSSVSVSDKELQNSASDVARESFNVAGNPQTSVAEEKIGS 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTS 420
           SR+VHSNITKREYMLRQKSLHVDKNFRTYGPKG SRAGK+ GLG LMLDKKLKSVSGST+
Sbjct: 361 SRRVHSNITKREYMLRQKSLHVDKNFRTYGPKGPSRAGKVTGLGSLMLDKKLKSVSGSTA 420

Query: 421 VNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMP 480
           VNFKN SLKISKAMGIDVAQDNGNHN +TIDIPSSSL FNLEN  T SPFSKINV SSMP
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGNHNPTTIDIPSSSLSFNLENNKTASPFSKINVLSSMP 480

Query: 481 APSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSLG 540
           APSSP ALPATNTSSALP  + DLSLSLP KSN+P+VP +CN E STSSFVEKPYEKSLG
Sbjct: 481 APSSPLALPATNTSSALPAAETDLSLSLPTKSNEPSVPISCNTEPSTSSFVEKPYEKSLG 540

Query: 541 QWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFP+DKKDEMVL LVPR RELQ+QL EW EWAN KVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPKDKKDEMVLKLVPRARELQSQLQEWMEWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660
           VERLKKEKQTLEENT KKLSEMEHALCKASGQVELANSAVRRLEVENAALRQ+MEVAKLR
Sbjct: 601 VERLKKEKQTLEENTKKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQL 720
           ATESAA++QEVSKREKKTL+ VQSWEK KMLFQEEHT EKRK+KQLIQELEQARD+QEQL
Sbjct: 661 ATESAATFQEVSKREKKTLVNVQSWEKTKMLFQEEHTVEKRKLKQLIQELEQARDVQEQL 720

Query: 721 EGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKE 780
           EGR K+E RAKDELL+QAASLRKEREQIE S+K KEDTIKLKAENNL+KYK DIQKLEKE
Sbjct: 721 EGRGKMEARAKDELLMQAASLRKEREQIEASLKAKEDTIKLKAENNLLKYKSDIQKLEKE 780

Query: 781 ISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTGG 840
           IS LRLKTDSSRIAALKRGIDGSYASRL DTRNSTDH ESW+PNVSESMKDLYEYSGTG 
Sbjct: 781 ISQLRLKTDSSRIAALKRGIDGSYASRLIDTRNSTDHNESWTPNVSESMKDLYEYSGTGS 840

Query: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYAR 900
           VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS IQRRIPVRY R
Sbjct: 841 VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYTR 900

Query: 901 S 902
           S
Sbjct: 901 S 901

BLAST of HG10012628 vs. TAIR 10
Match: AT4G03000.1 (RING/U-box superfamily protein )

HSP 1 Score: 468.8 bits (1205), Expect = 1.0e-131
Identity = 350/890 (39.33%), Postives = 503/890 (56.52%), Query Frame = 0

Query: 24  QEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSVGQTSGCDLCSISQ 83
           Q+KG +NKRK      L D +   ++S  E P YE  + K          S   LC    
Sbjct: 29  QDKGRKNKRK------LADPSPQNAASLTEFPRYELHSFK----------SQSPLCE--- 88

Query: 84  EFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDADWSDLTEAELEELVLNNLDTIFK 143
                                 N   G+L+ +E     W D     LE L+ +NL T+F+
Sbjct: 89  ----------------------NDSNGQLKAEESDSVGWDDPFACHLEGLLSSNLLTLFR 148

Query: 144 SAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNALAFLRSGQEIDQSREHYFED 203
           SA+ +I+  GY+E+V +KA+S S    G  D VSNIV++ L+FL+SG+++  SR++ FED
Sbjct: 149 SAMNQIMDCGYSEDVVLKAISSSRFYCGGTDLVSNIVNDTLSFLKSGKKVAGSRDYVFED 208

Query: 204 LQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDMNVSLACAMDSDPCNALVCDGTSN 263
           LQQL  Y L E + ++REVRP  ST +AMW LLI D+NV  A  +D+D    L     SN
Sbjct: 209 LQQLVAYSLVEKISLVREVRPSLSTDEAMWRLLICDLNVLKAFEVDAD---GLEGSSVSN 268

Query: 264 ESSSNSIPQLKAEVKTSEMNLPKPV---KPISPISCAHGSQSDGPATLGVPSITKSKDLL 323
            S S+  P        +E N PK      P +P+S     QS+        ++  SK+  
Sbjct: 269 ASKSSESP-------VAECNPPKSSDADNPKAPVSNTQSKQSEPVKFGNFANVNNSKN-P 328

Query: 324 FSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREY-MLRQKS 383
            +SG    KE+ + ++ + E + S    S TSVS+EK+ S RK     TK+E  MLRQKS
Sbjct: 329 HASGATPGKEVFSVSTASGEGTKSA---SLTSVSDEKLVSCRK---GRTKKEMAMLRQKS 388

Query: 384 LHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTSVNFKNTSLKI-SKAMGIDV 443
             V+K  RTY   G  +  K    GG +++K+ KS S   S   +N+S KI ++ M I +
Sbjct: 389 C-VEK-IRTYSKGGGYKTAK---FGGFLVEKRGKSASDLLSAQARNSSSKITTEVMKIPL 448

Query: 444 AQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMPAPSSPPALPATNTSSALP 503
           A+ +   + ST                                 S  PAL      +ALP
Sbjct: 449 AESSSTLSNST--------------------------------KSDSPALDVKEHVTALP 508

Query: 504 TTDIDLSLSLPAKS-NQPTVPFNCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMVLNLVP 563
             +    ++   KS ++P    + + + +   +   PY+ +LG + PR+K+DE++L LVP
Sbjct: 509 ANNAPAPVASEKKSGSEPEEKPSVSTKPAPDYYAAIPYDATLGIYIPRNKRDELILKLVP 568

Query: 564 RVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLEENTMK 623
           R+++LQ +L +WT+WAN KV QA  RL KD+ ELK L++EKEE E  +KEKQ LEENT+K
Sbjct: 569 RMKDLQKELQDWTDWANQKVKQATVRLLKDQPELKALRKEKEEAEEFRKEKQLLEENTIK 628

Query: 624 KLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLRATESAASYQEVSKREKK 683
           + SEME AL  A+ Q+E  N+ +RRLE+E + L+++ E A +RA+ESA S +E  +R ++
Sbjct: 629 RRSEMELALNNATNQLERTNNTIRRLELEQSLLKREREAANIRASESAESCREAKERVQR 688

Query: 684 TLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQLEGRWKLEERAKDELLVQ 743
            L   QSWE QK L QEE  +++ KV  L QE+ +A+  Q Q+E  WK E+ A  +L  Q
Sbjct: 689 LLKNSQSWEGQKNLLQEELKSQRDKVAGLQQEVAKAKTRQNQIEATWKQEKSATGKLTAQ 748

Query: 744 AASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKEISVLRLKTDSSRIAALK 803
           AA+L+KER ++E+  K +E+ IK KAEN++  Y ++I++L+ EIS L+LK+DS +IAALK
Sbjct: 749 AAALKKERGKLEELGKAEEERIKTKAENDVKYYIENIKRLDTEISKLKLKSDSLKIAALK 808

Query: 804 RGIDG------SYASRLTDTR-NSTDHKESWSPNVSESMKDLYEYSGTGGVKRERECVMC 863
           +GIDG      S  +  T+T+ NS    + W  N     K          +KRERECVMC
Sbjct: 809 KGIDGNNDGNKSGMNHTTNTKANSMASAKVWENNQGAESK----------IKRERECVMC 813

Query: 864 LSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYAR 901
           LSEEMSV+FLPCAHQV+C+ CN+LHEK+ M+DCPSCR+ IQRRI  R+AR
Sbjct: 869 LSEEMSVIFLPCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 813

BLAST of HG10012628 vs. TAIR 10
Match: AT4G03000.2 (RING/U-box superfamily protein )

HSP 1 Score: 468.8 bits (1205), Expect = 1.0e-131
Identity = 350/890 (39.33%), Postives = 503/890 (56.52%), Query Frame = 0

Query: 24  QEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSVGQTSGCDLCSISQ 83
           Q+KG +NKRK      L D +   ++S  E P YE  + K          S   LC    
Sbjct: 29  QDKGRKNKRK------LADPSPQNAASLTEFPRYELHSFK----------SQSPLCE--- 88

Query: 84  EFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDADWSDLTEAELEELVLNNLDTIFK 143
                                 N   G+L+ +E     W D     LE L+ +NL T+F+
Sbjct: 89  ----------------------NDSNGQLKAEESDSVGWDDPFACHLEGLLSSNLLTLFR 148

Query: 144 SAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNALAFLRSGQEIDQSREHYFED 203
           SA+ +I+  GY+E+V +KA+S S    G  D VSNIV++ L+FL+SG+++  SR++ FED
Sbjct: 149 SAMNQIMDCGYSEDVVLKAISSSRFYCGGTDLVSNIVNDTLSFLKSGKKVAGSRDYVFED 208

Query: 204 LQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDMNVSLACAMDSDPCNALVCDGTSN 263
           LQQL  Y L E + ++REVRP  ST +AMW LLI D+NV  A  +D+D    L     SN
Sbjct: 209 LQQLVAYSLVEKISLVREVRPSLSTDEAMWRLLICDLNVLKAFEVDAD---GLEGSSVSN 268

Query: 264 ESSSNSIPQLKAEVKTSEMNLPKPV---KPISPISCAHGSQSDGPATLGVPSITKSKDLL 323
            S S+  P        +E N PK      P +P+S     QS+        ++  SK+  
Sbjct: 269 ASKSSESP-------VAECNPPKSSDADNPKAPVSNTQSKQSEPVKFGNFANVNNSKN-P 328

Query: 324 FSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREY-MLRQKS 383
            +SG    KE+ + ++ + E + S    S TSVS+EK+ S RK     TK+E  MLRQKS
Sbjct: 329 HASGATPGKEVFSVSTASGEGTKSA---SLTSVSDEKLVSCRK---GRTKKEMAMLRQKS 388

Query: 384 LHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTSVNFKNTSLKI-SKAMGIDV 443
             V+K  RTY   G  +  K    GG +++K+ KS S   S   +N+S KI ++ M I +
Sbjct: 389 C-VEK-IRTYSKGGGYKTAK---FGGFLVEKRGKSASDLLSAQARNSSSKITTEVMKIPL 448

Query: 444 AQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMPAPSSPPALPATNTSSALP 503
           A+ +   + ST                                 S  PAL      +ALP
Sbjct: 449 AESSSTLSNST--------------------------------KSDSPALDVKEHVTALP 508

Query: 504 TTDIDLSLSLPAKS-NQPTVPFNCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMVLNLVP 563
             +    ++   KS ++P    + + + +   +   PY+ +LG + PR+K+DE++L LVP
Sbjct: 509 ANNAPAPVASEKKSGSEPEEKPSVSTKPAPDYYAAIPYDATLGIYIPRNKRDELILKLVP 568

Query: 564 RVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLEENTMK 623
           R+++LQ +L +WT+WAN KV QA  RL KD+ ELK L++EKEE E  +KEKQ LEENT+K
Sbjct: 569 RMKDLQKELQDWTDWANQKVKQATVRLLKDQPELKALRKEKEEAEEFRKEKQLLEENTIK 628

Query: 624 KLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLRATESAASYQEVSKREKK 683
           + SEME AL  A+ Q+E  N+ +RRLE+E + L+++ E A +RA+ESA S +E  +R ++
Sbjct: 629 RRSEMELALNNATNQLERTNNTIRRLELEQSLLKREREAANIRASESAESCREAKERVQR 688

Query: 684 TLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQLEGRWKLEERAKDELLVQ 743
            L   QSWE QK L QEE  +++ KV  L QE+ +A+  Q Q+E  WK E+ A  +L  Q
Sbjct: 689 LLKNSQSWEGQKNLLQEELKSQRDKVAGLQQEVAKAKTRQNQIEATWKQEKSATGKLTAQ 748

Query: 744 AASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKEISVLRLKTDSSRIAALK 803
           AA+L+KER ++E+  K +E+ IK KAEN++  Y ++I++L+ EIS L+LK+DS +IAALK
Sbjct: 749 AAALKKERGKLEELGKAEEERIKTKAENDVKYYIENIKRLDTEISKLKLKSDSLKIAALK 808

Query: 804 RGIDG------SYASRLTDTR-NSTDHKESWSPNVSESMKDLYEYSGTGGVKRERECVMC 863
           +GIDG      S  +  T+T+ NS    + W  N     K          +KRERECVMC
Sbjct: 809 KGIDGNNDGNKSGMNHTTNTKANSMASAKVWENNQGAESK----------IKRERECVMC 813

Query: 864 LSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYAR 901
           LSEEMSV+FLPCAHQV+C+ CN+LHEK+ M+DCPSCR+ IQRRI  R+AR
Sbjct: 869 LSEEMSVIFLPCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 813

BLAST of HG10012628 vs. TAIR 10
Match: AT1G03365.1 (RING/U-box superfamily protein )

HSP 1 Score: 447.2 bits (1149), Expect = 3.1e-125
Identity = 339/889 (38.13%), Postives = 498/889 (56.02%), Query Frame = 0

Query: 20  SMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSVGQTSGCDLC 79
           +++ QEKG +NKRK      L D ++  +SS  E P YE  +                  
Sbjct: 24  TVSPQEKGRKNKRK------LADPSQPNASSLTEFPPYELPS------------------ 83

Query: 80  SISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDADWSDLTEAELEELVLNNLD 139
                    LK    LS  GS    +     +L+V+  +  +W D     LEEL+ +NL 
Sbjct: 84  ---------LKPQNHLSGNGS----VGEVSNQLQVEVSESVEWDDPFACHLEELLSSNLL 143

Query: 140 TIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNALAFLRSGQEIDQSREH 199
           T+F   +K+++  GYT++  +KAVSR  +  G  + +SNIV+N L+ L++G E   S ++
Sbjct: 144 TLFLDTMKQLIDLGYTDDEVLKAVSRCRLYCGGNNLLSNIVNNTLSALKTGDEGAGSGDY 203

Query: 200 YFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLISDMNVSLACAMDSDPCNALVCD 259
            FEDLQQL  Y L E++ +++EVRP  ST +AMW LL+ D+NV  A   + D    LV  
Sbjct: 204 VFEDLQQLVSYTLVEMISLIKEVRPSLSTVEAMWRLLMCDLNVLQAFEAEGD---GLV-- 263

Query: 260 GTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPISCAHGSQSDGPATLGVPSITKSKDL 319
            +S  S S S+       K+S+ + PKP     P S    ++++ P   G    T +   
Sbjct: 264 SSSKLSDSESLGAESNPPKSSDPDNPKP-----PQSDPQSNRNE-PLKFGNFPNTPNSKK 323

Query: 320 LFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREY-MLRQK 379
             SSG    KE+ + ++ + +    +   S T VS+EK+ S RK     TK+E  MLRQK
Sbjct: 324 TQSSGTTPGKEVCSGSTVSCQ---GMRSTSFTLVSDEKLVSCRK---GRTKKEIAMLRQK 383

Query: 380 SLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLKSVSGSTSVNFKNTSLKISKAMGIDV 439
           S  V+K  RTY      +A K A +G  +L+K++KS   S+    +N+S KI+  +G+ V
Sbjct: 384 SC-VEK-IRTYSKGSGYKAAKFASVGSFLLEKRVKS---SSEFVPRNSSSKITAEIGVKV 443

Query: 440 --AQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKINVPSSMPAPSSP----PALPATN 499
             A+D+G        + S  +  + +   T  P   +   S     S      P+     
Sbjct: 444 SLAEDSGCFVRKNSKLDSPVVVVDAKGYITALPARSVKSASKKKTGSESVTLIPSASEKK 503

Query: 500 TSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMV 559
           + S++P+T      S     ++     + +A+ +   +   PY+ +LG + PRDKKDE++
Sbjct: 504 SDSSIPST------SEKKSGSESEEKASVSAKLAPDYYAGIPYDAALGIYVPRDKKDELI 563

Query: 560 LNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLE 619
           L LVPRV +LQN+L  WT+WAN KV +A  RL KD+ ELK L++E+EE E+ KKEKQ LE
Sbjct: 564 LKLVPRVNDLQNELQVWTDWANQKVKEATGRLLKDQPELKALRKEREEAEQYKKEKQLLE 623

Query: 620 ENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLRATESAASYQEVS 679
           ENT K+LSEM+ AL  A+ Q+E A +   RLE+E + L+++ME AK++A ESA S++E  
Sbjct: 624 ENTRKRLSEMDFALKNATSQLEKAFNTAHRLELEQSILKKEMEAAKIKAVESAESFREAK 683

Query: 680 KREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQELEQARDLQEQLEGRWKLEERAKD 739
           +R +++L  + SWE QK++ QEE   ++ KV  L +E+ +A++ Q Q+E   K E  AK 
Sbjct: 684 ERGERSLKDIHSWEGQKIMLQEELKGQREKVTVLQKEVTKAKNRQNQIEAALKQERTAKG 743

Query: 740 ELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKYKDDIQKLEKEISVLRLKTDSSR 799
           +L  QA+ +RKE +++E   KV+E+ IK KAE ++  Y D+I++LE+EIS L+LK+D SR
Sbjct: 744 KLSAQASLIRKETKELEALGKVEEERIKGKAETDVKYYIDNIKRLEREISELKLKSDYSR 803

Query: 800 IAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMKDLYEYSGTGGVKRERECVMCLS 859
           I ALK+G                          SES     E  G   VKRERECVMCLS
Sbjct: 804 IIALKKG-------------------------SSESKATKRESLGMPKVKRERECVMCLS 822

Query: 860 EEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSLIQRRIPVRYARS 902
           EEMSV+FLPCAHQV+C  CN+LHEK+GM DCPSCR  I RRI  R+ARS
Sbjct: 864 EEMSVIFLPCAHQVLCFKCNQLHEKEGMMDCPSCRGTIHRRIQARFARS 822

BLAST of HG10012628 vs. TAIR 10
Match: AT2G35330.1 (RING/U-box superfamily protein )

HSP 1 Score: 297.4 bits (760), Expect = 4.0e-80
Identity = 258/793 (32.53%), Postives = 409/793 (51.58%), Query Frame = 0

Query: 122 WSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVD 181
           W   TE +LE+++L +L+ ++  AI K+V SGY E+VA++AV  +G C+G  D ++NI+ 
Sbjct: 98  WGYCTEEQLEDILLKHLEYLYNEAISKLVGSGYDEDVALRAVLSNGYCYGGMDVMTNILH 157

Query: 182 NALAFLRS----GQEI---DQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWC 241
           N+LA+L+S    G  +   DQS E  F DL+QLE+Y LA +V +L++V+P  S GDAMWC
Sbjct: 158 NSLAYLKSNTGEGSNVNNEDQS-ETVFTDLRQLEEYSLAGMVYLLQQVKPNLSKGDAMWC 217

Query: 242 LLISDMNVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPI 301
           LL+S+++V  A  MD           +S +  S+++    A    + +        I+P 
Sbjct: 218 LLMSELHVGRASTMDIP---------SSGKGDSSNVGVGGASSTVNGVG-----GAIAPA 277

Query: 302 SCA-HGSQSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSV 361
            C  HG    G            K   FS                    FS+  +S+   
Sbjct: 278 LCRFHGGWGFG----------NGKGPKFSG-----------------NGFSL--HSEELT 337

Query: 362 SEEKIGSSRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLK 421
            + +I   R+ + + + +  +LR+                 + A   AG    M  KK  
Sbjct: 338 LQREIDCPRRFNLSPSMKS-LLRE-----------------NVAAFAAGYRASMEQKKQV 397

Query: 422 SVSGSTSVNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKI 481
            +   TS     TSL  + A                                        
Sbjct: 398 QMQSETS----GTSLSCTAA---------------------------------------- 457

Query: 482 NVPSSMPAPSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEK 541
                           AT++                 K  QP V     +E   SS +EK
Sbjct: 458 ----------------ATHSE----------------KCEQPHV---FGSEECFSSVLEK 517

Query: 542 PYEKSLG---QWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAE 601
             + +L       P + KD+ ++ L+ +V++L+ QL E  +WA  K MQAA+++S + +E
Sbjct: 518 FRDLNLDDNVDSAPEELKDDALIGLLQQVQDLKKQLKERKDWAQKKAMQAAQKVSDELSE 577

Query: 602 LKTLKQEKEEVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAAL 661
           LK+L+ E+EE++R+KK KQT E++T+KKLSEME+AL KASGQV+ AN+ VR LE E+A +
Sbjct: 578 LKSLRSEREEIQRVKKGKQTREDSTLKKLSEMENALRKASGQVDKANAVVRALENESAEI 637

Query: 662 RQDMEVAKLRATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQEL 721
           R +ME +KL A+ES  +  E SK+EKK L K+ +WEKQKM  Q+E TAEK K+K L + L
Sbjct: 638 RAEMEASKLSASESLTACMEASKKEKKCLKKLLAWEKQKMKLQDEITAEKEKIKALNRAL 697

Query: 722 EQARDLQEQLEGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKY 781
            Q    +++ E +W+ E++AK+++L Q    ++ +E IE S K K ++++LK E +  ++
Sbjct: 698 AQITQEEKEYEAKWRQEQKAKEQVLAQVEEEQRSKEAIEASNKRKVESLRLKIEIDFQRH 738

Query: 782 KDDIQKLEKEISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMK 841
           KDD+Q+LE+E+S  RL   SS  ++L+       ++  + T+  +D  +S    +S+ ++
Sbjct: 758 KDDLQRLEQELS--RLNKASSTDSSLQ-------SNNTSHTKVKSD--KSKGETMSKLLE 738

Query: 842 DLYEYSGT--GGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS 901
           +L    G+       +REC++C+ +E+SVVFLPCAHQVVC +C++     G   CP CR+
Sbjct: 818 ELNRLDGSYEKEANYDRECLICMKDEVSVVFLPCAHQVVCASCSDSFMGSGKATCPCCRA 738

BLAST of HG10012628 vs. TAIR 10
Match: AT2G35330.2 (RING/U-box superfamily protein )

HSP 1 Score: 297.4 bits (760), Expect = 4.0e-80
Identity = 258/793 (32.53%), Postives = 409/793 (51.58%), Query Frame = 0

Query: 122 WSDLTEAELEELVLNNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVD 181
           W   TE +LE+++L +L+ ++  AI K+V SGY E+VA++AV  +G C+G  D ++NI+ 
Sbjct: 71  WGYCTEEQLEDILLKHLEYLYNEAISKLVGSGYDEDVALRAVLSNGYCYGGMDVMTNILH 130

Query: 182 NALAFLRS----GQEI---DQSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWC 241
           N+LA+L+S    G  +   DQS E  F DL+QLE+Y LA +V +L++V+P  S GDAMWC
Sbjct: 131 NSLAYLKSNTGEGSNVNNEDQS-ETVFTDLRQLEEYSLAGMVYLLQQVKPNLSKGDAMWC 190

Query: 242 LLISDMNVSLACAMDSDPCNALVCDGTSNESSSNSIPQLKAEVKTSEMNLPKPVKPISPI 301
           LL+S+++V  A  MD           +S +  S+++    A    + +        I+P 
Sbjct: 191 LLMSELHVGRASTMDIP---------SSGKGDSSNVGVGGASSTVNGVG-----GAIAPA 250

Query: 302 SCA-HGSQSDGPATLGVPSITKSKDLLFSSGPLSEKELQNSTSDNVEESFSVAGNSQTSV 361
            C  HG    G            K   FS                    FS+  +S+   
Sbjct: 251 LCRFHGGWGFG----------NGKGPKFSG-----------------NGFSL--HSEELT 310

Query: 362 SEEKIGSSRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLAGLGGLMLDKKLK 421
            + +I   R+ + + + +  +LR+                 + A   AG    M  KK  
Sbjct: 311 LQREIDCPRRFNLSPSMKS-LLRE-----------------NVAAFAAGYRASMEQKKQV 370

Query: 422 SVSGSTSVNFKNTSLKISKAMGIDVAQDNGNHNLSTIDIPSSSLPFNLENINTVSPFSKI 481
            +   TS     TSL  + A                                        
Sbjct: 371 QMQSETS----GTSLSCTAA---------------------------------------- 430

Query: 482 NVPSSMPAPSSPPALPATNTSSALPTTDIDLSLSLPAKSNQPTVPFNCNAESSTSSFVEK 541
                           AT++                 K  QP V     +E   SS +EK
Sbjct: 431 ----------------ATHSE----------------KCEQPHV---FGSEECFSSVLEK 490

Query: 542 PYEKSLG---QWFPRDKKDEMVLNLVPRVRELQNQLHEWTEWANHKVMQAARRLSKDKAE 601
             + +L       P + KD+ ++ L+ +V++L+ QL E  +WA  K MQAA+++S + +E
Sbjct: 491 FRDLNLDDNVDSAPEELKDDALIGLLQQVQDLKKQLKERKDWAQKKAMQAAQKVSDELSE 550

Query: 602 LKTLKQEKEEVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAAL 661
           LK+L+ E+EE++R+KK KQT E++T+KKLSEME+AL KASGQV+ AN+ VR LE E+A +
Sbjct: 551 LKSLRSEREEIQRVKKGKQTREDSTLKKLSEMENALRKASGQVDKANAVVRALENESAEI 610

Query: 662 RQDMEVAKLRATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKQLIQEL 721
           R +ME +KL A+ES  +  E SK+EKK L K+ +WEKQKM  Q+E TAEK K+K L + L
Sbjct: 611 RAEMEASKLSASESLTACMEASKKEKKCLKKLLAWEKQKMKLQDEITAEKEKIKALNRAL 670

Query: 722 EQARDLQEQLEGRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKY 781
            Q    +++ E +W+ E++AK+++L Q    ++ +E IE S K K ++++LK E +  ++
Sbjct: 671 AQITQEEKEYEAKWRQEQKAKEQVLAQVEEEQRSKEAIEASNKRKVESLRLKIEIDFQRH 711

Query: 782 KDDIQKLEKEISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMK 841
           KDD+Q+LE+E+S  RL   SS  ++L+       ++  + T+  +D  +S    +S+ ++
Sbjct: 731 KDDLQRLEQELS--RLNKASSTDSSLQ-------SNNTSHTKVKSD--KSKGETMSKLLE 711

Query: 842 DLYEYSGT--GGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRS 901
           +L    G+       +REC++C+ +E+SVVFLPCAHQVVC +C++     G   CP CR+
Sbjct: 791 ELNRLDGSYEKEANYDRECLICMKDEVSVVFLPCAHQVVCASCSDSFMGSGKATCPCCRA 711