HG10009567 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10009567
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPolcalcin Jun o
LocationChr06: 7541032 .. 7541577 (-)
RNA-Seq ExpressionHG10009567
SyntenyHG10009567
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATTGAAATCTCTTTTCTCCCGGAAGAAGAAATCGGATTCCTCCACCGATTCACACCTTGGATCTCGAACTCCCTCTCTCAATTTGCGAGCGCAGATCGACGAACTCGAGCAAGTTTTCAAGAAATTTGATGTCAATGGCGACGGTAAGATCTGTTCCTCTGAACTCGGATCTATTATGGGGAGTCTAGGGCAGCCGGCCACAGAGGAGGAGCTTCAGAATATGATTAAGGAGGTAGATGCCGACGGCGATGGCTACATTGACCTCGAAGAGTTCATTGAACTTAATACTAAGGGCGTTGATTCCGATGAGGTGCTTGAGAATCTGAAGGACGCGTTCTCTGTGTACGACATTGACGGAAACGGATCCATCACGGCCGAGGAATTGCACGAGGTTTTGAAAAGCTTGGGCGATGATTGCTCTTTGGCGGATTGCAGGAAGATGATTAACGGCGTCGATAAAAACGGTGACGGTATGATTAGTTTCGATGAATTCAAAGTGATGATGATGAGCGGTTCTCGATCGCACGGCTTCAATGGTTAA

mRNA sequence

ATGGGATTGAAATCTCTTTTCTCCCGGAAGAAGAAATCGGATTCCTCCACCGATTCACACCTTGGATCTCGAACTCCCTCTCTCAATTTGCGAGCGCAGATCGACGAACTCGAGCAAGTTTTCAAGAAATTTGATGTCAATGGCGACGGTAAGATCTGTTCCTCTGAACTCGGATCTATTATGGGGAGTCTAGGGCAGCCGGCCACAGAGGAGGAGCTTCAGAATATGATTAAGGAGGTAGATGCCGACGGCGATGGCTACATTGACCTCGAAGAGTTCATTGAACTTAATACTAAGGGCGTTGATTCCGATGAGGTGCTTGAGAATCTGAAGGACGCGTTCTCTGTGTACGACATTGACGGAAACGGATCCATCACGGCCGAGGAATTGCACGAGGTTTTGAAAAGCTTGGGCGATGATTGCTCTTTGGCGGATTGCAGGAAGATGATTAACGGCGTCGATAAAAACGGTGACGGTATGATTAGTTTCGATGAATTCAAAGTGATGATGATGAGCGGTTCTCGATCGCACGGCTTCAATGGTTAA

Coding sequence (CDS)

ATGGGATTGAAATCTCTTTTCTCCCGGAAGAAGAAATCGGATTCCTCCACCGATTCACACCTTGGATCTCGAACTCCCTCTCTCAATTTGCGAGCGCAGATCGACGAACTCGAGCAAGTTTTCAAGAAATTTGATGTCAATGGCGACGGTAAGATCTGTTCCTCTGAACTCGGATCTATTATGGGGAGTCTAGGGCAGCCGGCCACAGAGGAGGAGCTTCAGAATATGATTAAGGAGGTAGATGCCGACGGCGATGGCTACATTGACCTCGAAGAGTTCATTGAACTTAATACTAAGGGCGTTGATTCCGATGAGGTGCTTGAGAATCTGAAGGACGCGTTCTCTGTGTACGACATTGACGGAAACGGATCCATCACGGCCGAGGAATTGCACGAGGTTTTGAAAAGCTTGGGCGATGATTGCTCTTTGGCGGATTGCAGGAAGATGATTAACGGCGTCGATAAAAACGGTGACGGTATGATTAGTTTCGATGAATTCAAAGTGATGATGATGAGCGGTTCTCGATCGCACGGCTTCAATGGTTAA

Protein sequence

MGLKSLFSRKKKSDSSTDSHLGSRTPSLNLRAQIDELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSGSRSHGFNG
Homology
BLAST of HG10009567 vs. NCBI nr
Match: XP_038875473.1 (probable calcium-binding protein CML25 [Benincasa hispida])

HSP 1 Score: 325.5 bits (833), Expect = 3.0e-85
Identity = 171/185 (92.43%), Postives = 172/185 (92.97%), Query Frame = 0

Query: 1   MGLKSLFSRKKKSDSSTDSHLGSRT----PSLNLRAQIDELEQVFKKFDVNGDGKICSSE 60
           MG KSLFSRKKKS SSTDS LGS T     SLN RAQI ELEQVFKKFDVNGDGKICSSE
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTVPILGSLNSRAQIAELEQVFKKFDVNGDGKICSSE 60

Query: 61  LGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSV 120
           LGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL+EFIELNTKGVDSDEVLENLKDAFSV
Sbjct: 61  LGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSV 120

Query: 121 YDIDGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSGSRS 180
           YDIDGNGSITAEELHEVLKSLGDDCSLADCRKMI GVDKNGDGMISFDEFKVMMMSGSRS
Sbjct: 121 YDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRS 180

Query: 181 HGFNG 182
            GFNG
Sbjct: 181 QGFNG 185

BLAST of HG10009567 vs. NCBI nr
Match: XP_011655449.1 (probable calcium-binding protein CML25 [Cucumis sativus] >KGN51467.1 hypothetical protein Csa_008232 [Cucumis sativus])

HSP 1 Score: 322.0 bits (824), Expect = 3.3e-84
Identity = 168/182 (92.31%), Postives = 169/182 (92.86%), Query Frame = 0

Query: 1   MGLKSLFSRKKKSDSSTDSHLGSRT-PSLNLRAQIDELEQVFKKFDVNGDGKICSSELGS 60
           MG KSLFSRKKKS SSTDS LGS T P L  R QI ELEQVF KFDVNGDGKICSSELGS
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60

Query: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDI 120
           IMGSLGQPATEEELQNMIKEVDADGDGYIDL+EFIELNTKGVDSDEVLENLKDAFSVYDI
Sbjct: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSGSRSHGF 180
           DGNGSITAEELHEVLKSLGDDCSLADCRKMI GVDKNGDGMISFDEFKVMMMSGSRS GF
Sbjct: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 180

Query: 181 NG 182
           NG
Sbjct: 181 NG 182

BLAST of HG10009567 vs. NCBI nr
Match: XP_022963580.1 (probable calcium-binding protein CML25 [Cucurbita moschata])

HSP 1 Score: 318.5 bits (815), Expect = 3.6e-83
Identity = 165/188 (87.77%), Postives = 171/188 (90.96%), Query Frame = 0

Query: 1   MGLKSLFSRKKKSDSSTDSH--------LGSRTPSLNLRAQIDELEQVFKKFDVNGDGKI 60
           MG KSLFSRKKKSDSS+DS         LGSRTPS N RAQI ELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVD+NGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

BLAST of HG10009567 vs. NCBI nr
Match: XP_022990613.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 318.5 bits (815), Expect = 3.6e-83
Identity = 165/188 (87.77%), Postives = 171/188 (90.96%), Query Frame = 0

Query: 1   MGLKSLFSRKKKSDSSTDSH--------LGSRTPSLNLRAQIDELEQVFKKFDVNGDGKI 60
           MG KSLFSRKKKSDSS+DS         LGSRTPS N RAQI ELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVD+NGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

BLAST of HG10009567 vs. NCBI nr
Match: XP_023513887.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 318.2 bits (814), Expect = 4.7e-83
Identity = 165/186 (88.71%), Postives = 170/186 (91.40%), Query Frame = 0

Query: 1   MGLKSLFSRKKKSDSSTDSH--------LGSRTPSLNLRAQIDELEQVFKKFDVNGDGKI 60
           MG KSLFSRKKKSDSS+DS         LGSRTPS N RAQI ELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMS 179
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVD+NGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

BLAST of HG10009567 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 3.9e-56
Identity = 112/182 (61.54%), Postives = 138/182 (75.82%), Query Frame = 0

Query: 6   LFSRKKKSDSSTDSHLGSRTPSLNL------RAQIDELEQVFKKFDVNGDGKICSSELGS 65
           +F++ + S+  + S++G    S  L      + +I ELE VFKKFDVNGDGKI S ELG+
Sbjct: 1   MFNKNQGSNGGSSSNVGIGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGA 60

Query: 66  IMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDI 125
           IM SLG    EEEL+  I E+D  GDGYI+ EEF+ELNTKG+D ++VLENLKDAFSVYDI
Sbjct: 61  IMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDI 120

Query: 126 DGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSGSRSHGF 182
           DGNGSI+AEELHEVL+SLGD+CS+A+CRKMI GVDK+GDG I F+EFK+MM  GSR    
Sbjct: 121 DGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMGSRRDNV 180

BLAST of HG10009567 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.8e-37
Identity = 87/176 (49.43%), Postives = 117/176 (66.48%), Query Frame = 0

Query: 3   LKSLFSRKKKSDS----STDSHLGSRTPSLNLRAQIDELEQVFKKFDVNGDGKICSSELG 62
           + +LF R+  S S      D   G  +P+       +E+E+VF+KFD NGDG+I  SELG
Sbjct: 6   MPALFRRRSGSKSPPLPQADPASGGGSPA---PTPEEEMERVFRKFDANGDGRISRSELG 65

Query: 63  SIMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELN-TKGVDSDEVLENLKDAFSVY 122
           ++  SLG  AT++EL  M+ E DADGDG+I L+EF  LN T   D+  V E+L+ AF V+
Sbjct: 66  ALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVF 125

Query: 123 DIDGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSG 174
           D DGNG+I+A EL  VL  LG+  ++  CR+MI GVD+NGDG+ISF+EFKVMM  G
Sbjct: 126 DADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGG 178

BLAST of HG10009567 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-35
Identity = 75/157 (47.77%), Postives = 109/157 (69.43%), Query Frame = 0

Query: 16  STDSHLGSRTPSLNLRAQIDELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQN 75
           S+D    + +  + +   + ELE+VFKKFD NGDGKI  SEL  I+ SLG    E E++ 
Sbjct: 6   SSDGSKSACSGEVVMEQSVHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKA 65

Query: 76  MIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLK 135
           M++E DADGDGY+ L+EF++LN KG      +++LK+AF V+D D NGSI+A EL   L+
Sbjct: 66  MMEEADADGDGYVSLQEFVDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLE 125

Query: 136 SLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMS 173
           S+G+ C++ + + +I+ VDKNGDG+IS +EF+ MM S
Sbjct: 126 SVGEPCTIEESKNIIHNVDKNGDGLISVEEFQTMMTS 158

BLAST of HG10009567 vs. ExPASy Swiss-Prot
Match: A0T2M3 (Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 2.2e-35
Identity = 76/160 (47.50%), Postives = 108/160 (67.50%), Query Frame = 0

Query: 13  SDSSTDSHLGSRTPSLNLRAQIDELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEE 72
           SD S  +  G R     L   + ELE+VFKKFD NGDGKI  SEL  I+ S+G    E E
Sbjct: 7   SDESKSASSGKRV----LEQSVHELEEVFKKFDANGDGKISGSELADILRSMGSEVDEAE 66

Query: 73  LQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHE 132
           ++ M++E D DGDGY+ L+EF++LN KG      +++LK+AF V+D D NG+I+  EL E
Sbjct: 67  VKAMMEEADTDGDGYVSLQEFVDLNIKGA----TVKDLKNAFKVFDRDCNGTISPAELCE 126

Query: 133 VLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMS 173
            LKS+G+ C++ + + +I+ VDKNGDG+I+ +EF+ MM S
Sbjct: 127 TLKSVGEPCTIEESKNIIHNVDKNGDGLINVEEFQTMMTS 158

BLAST of HG10009567 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.2e-34
Identity = 69/151 (45.70%), Postives = 107/151 (70.86%), Query Frame = 0

Query: 23  SRTPSLNLRAQIDELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDA 82
           S+  S N    ++++++VF++FD N DGKI   EL  ++G+L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 83  DGDGYIDLEEFIEL--NTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDD 142
           DG+G+IDL+EF+ L   +    ++  + +LK+AF +YD+D NG I+A ELH V+K+LG+ 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 143 CSLADCRKMINGVDKNGDGMISFDEFKVMMM 172
           CS+ DC++MIN VD +GDG + F+EFK MMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMM 152

BLAST of HG10009567 vs. ExPASy TrEMBL
Match: A0A0A0KST2 (Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.6e-84
Identity = 168/182 (92.31%), Postives = 169/182 (92.86%), Query Frame = 0

Query: 1   MGLKSLFSRKKKSDSSTDSHLGSRT-PSLNLRAQIDELEQVFKKFDVNGDGKICSSELGS 60
           MG KSLFSRKKKS SSTDS LGS T P L  R QI ELEQVF KFDVNGDGKICSSELGS
Sbjct: 1   MGFKSLFSRKKKSHSSTDSPLGSTTLPILGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60

Query: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDI 120
           IMGSLGQPATEEELQNMIKEVDADGDGYIDL+EFIELNTKGVDSDEVLENLKDAFSVYDI
Sbjct: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVYDI 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSGSRSHGF 180
           DGNGSITAEELHEVLKSLGDDCSLADCRKMI GVDKNGDGMISFDEFKVMMMSGSRS GF
Sbjct: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSQGF 180

Query: 181 NG 182
           NG
Sbjct: 181 NG 182

BLAST of HG10009567 vs. ExPASy TrEMBL
Match: A0A6J1JTS9 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487441 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.7e-83
Identity = 165/188 (87.77%), Postives = 171/188 (90.96%), Query Frame = 0

Query: 1   MGLKSLFSRKKKSDSSTDSH--------LGSRTPSLNLRAQIDELEQVFKKFDVNGDGKI 60
           MG KSLFSRKKKSDSS+DS         LGSRTPS N RAQI ELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVD+NGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

BLAST of HG10009567 vs. ExPASy TrEMBL
Match: A0A6J1HID9 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111463873 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.7e-83
Identity = 165/188 (87.77%), Postives = 171/188 (90.96%), Query Frame = 0

Query: 1   MGLKSLFSRKKKSDSSTDSH--------LGSRTPSLNLRAQIDELEQVFKKFDVNGDGKI 60
           MG KSLFSRKKKSDSS+DS         LGSRTPS N RAQI ELEQVFKKFDVNGDGKI
Sbjct: 1   MGFKSLFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKI 60

Query: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKD 120
           CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDSDEVL+NLKD
Sbjct: 61  CSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKD 120

Query: 121 AFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMS 180
           AFSVYD+DGNGSITAEELHEVLKSLGDDCSLADCR MI+GVD+NGDGMISFDEFK MMMS
Sbjct: 121 AFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMS 180

BLAST of HG10009567 vs. ExPASy TrEMBL
Match: A0A5A7TS48 (Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3042G00010 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.0e-83
Identity = 165/182 (90.66%), Postives = 167/182 (91.76%), Query Frame = 0

Query: 1   MGLKSLFSRKKKSDSSTDSHLGSRT-PSLNLRAQIDELEQVFKKFDVNGDGKICSSELGS 60
           MG KSLFSRKKKS  STDS LGS T P    R QI ELEQVF KFDVNGDGKICSSELGS
Sbjct: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60

Query: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDI 120
           IMGSLGQPATEEEL NMIKEVDADGDGYIDL+EFIELNTKGVDS+EVLENLKDAFSVYDI
Sbjct: 61  IMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDI 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSGSRSHGF 180
           DGNGSITAEELHEVLKSLGDDCSLADCRKMI GVDKNGDGMISFDEFKVMMMSGSRSHGF
Sbjct: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSHGF 180

Query: 181 NG 182
           NG
Sbjct: 181 NG 182

BLAST of HG10009567 vs. ExPASy TrEMBL
Match: A0A1S3CSR2 (probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103504532 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.0e-83
Identity = 165/182 (90.66%), Postives = 167/182 (91.76%), Query Frame = 0

Query: 1   MGLKSLFSRKKKSDSSTDSHLGSRT-PSLNLRAQIDELEQVFKKFDVNGDGKICSSELGS 60
           MG KSLFSRKKKS  STDS LGS T P    R QI ELEQVF KFDVNGDGKICSSELGS
Sbjct: 1   MGFKSLFSRKKKSHPSTDSPLGSTTLPIFGSRTQIAELEQVFNKFDVNGDGKICSSELGS 60

Query: 61  IMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDI 120
           IMGSLGQPATEEEL NMIKEVDADGDGYIDL+EFIELNTKGVDS+EVLENLKDAFSVYDI
Sbjct: 61  IMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDI 120

Query: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSGSRSHGF 180
           DGNGSITAEELHEVLKSLGDDCSLADCRKMI GVDKNGDGMISFDEFKVMMMSGSRSHGF
Sbjct: 121 DGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSRSHGF 180

Query: 181 NG 182
           NG
Sbjct: 181 NG 182

BLAST of HG10009567 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 219.2 bits (557), Expect = 2.8e-57
Identity = 112/182 (61.54%), Postives = 138/182 (75.82%), Query Frame = 0

Query: 6   LFSRKKKSDSSTDSHLGSRTPSLNL------RAQIDELEQVFKKFDVNGDGKICSSELGS 65
           +F++ + S+  + S++G    S  L      + +I ELE VFKKFDVNGDGKI S ELG+
Sbjct: 1   MFNKNQGSNGGSSSNVGIGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGA 60

Query: 66  IMGSLGQPATEEELQNMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDI 125
           IM SLG    EEEL+  I E+D  GDGYI+ EEF+ELNTKG+D ++VLENLKDAFSVYDI
Sbjct: 61  IMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDI 120

Query: 126 DGNGSITAEELHEVLKSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSGSRSHGF 182
           DGNGSI+AEELHEVL+SLGD+CS+A+CRKMI GVDK+GDG I F+EFK+MM  GSR    
Sbjct: 121 DGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMGSRRDNV 180

BLAST of HG10009567 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 146.4 bits (368), Expect = 2.3e-35
Identity = 69/151 (45.70%), Postives = 107/151 (70.86%), Query Frame = 0

Query: 23  SRTPSLNLRAQIDELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDA 82
           S+  S N    ++++++VF++FD N DGKI   EL  ++G+L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 83  DGDGYIDLEEFIEL--NTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDD 142
           DG+G+IDL+EF+ L   +    ++  + +LK+AF +YD+D NG I+A ELH V+K+LG+ 
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 143 CSLADCRKMINGVDKNGDGMISFDEFKVMMM 172
           CS+ DC++MIN VD +GDG + F+EFK MMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMM 152

BLAST of HG10009567 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 145.2 bits (365), Expect = 5.1e-35
Identity = 69/144 (47.92%), Postives = 106/144 (73.61%), Query Frame = 0

Query: 34  IDELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLEEF 93
           +D++++VF++FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+G+IDL+EF
Sbjct: 15  MDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEF 74

Query: 94  IELNTKGV----DSDEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKM 153
           + L   G+    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG+ CS+ DC+KM
Sbjct: 75  VALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKM 134

Query: 154 INGVDKNGDGMISFDEFKVMMMSG 174
           I+ VD +GDG ++FDEFK MM +G
Sbjct: 135 ISKVDIDGDGCVNFDEFKKMMSNG 158

BLAST of HG10009567 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.4 bits (337), Expect = 9.0e-32
Identity = 72/160 (45.00%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 15  SSTDSHLGSRTPSLNLRAQIDELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQ 74
           +S +      TP+    A  +EL++VF +FD NGDGKI   ELG +  ++G   TE EL 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 75  NMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVL 134
            +++EVD D DGYI+L+EF  L      + E+    +DAF +YD D NG I+A ELH+VL
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVL 121

Query: 135 KSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSGS 175
             LG  CS+ DC +MI  VD +GDG ++F+EF+ MM S S
Sbjct: 122 NRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

BLAST of HG10009567 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.4 bits (337), Expect = 9.0e-32
Identity = 72/160 (45.00%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 15  SSTDSHLGSRTPSLNLRAQIDELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQ 74
           +S +      TP+    A  +EL++VF +FD NGDGKI   ELG +  ++G   TE EL 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 75  NMIKEVDADGDGYIDLEEFIELNTKGVDSDEVLENLKDAFSVYDIDGNGSITAEELHEVL 134
            +++EVD D DGYI+L+EF  L      + E+    +DAF +YD D NG I+A ELH+VL
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVL 121

Query: 135 KSLGDDCSLADCRKMINGVDKNGDGMISFDEFKVMMMSGS 175
             LG  CS+ DC +MI  VD +GDG ++F+EF+ MM S S
Sbjct: 122 NRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038875473.13.0e-8592.43probable calcium-binding protein CML25 [Benincasa hispida][more]
XP_011655449.13.3e-8492.31probable calcium-binding protein CML25 [Cucumis sativus] >KGN51467.1 hypothetica... [more]
XP_022963580.13.6e-8387.77probable calcium-binding protein CML25 [Cucurbita moschata][more]
XP_022990613.13.6e-8387.77probable calcium-binding protein CML25 [Cucurbita maxima][more]
XP_023513887.14.7e-8388.71probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FYK23.9e-5661.54Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ... [more]
Q8RZB51.8e-3749.43Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
O649431.3e-3547.77Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2[more]
A0T2M32.2e-3547.50Polcalcin Cup a 4 OS=Hesperocyparis arizonica OX=49011 PE=1 SV=1[more]
Q9C8Y13.2e-3445.70Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KST21.6e-8492.31Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1[more]
A0A6J1JTS91.7e-8387.77probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114874... [more]
A0A6J1HID91.7e-8387.77probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A5A7TS483.0e-8390.66Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CSR23.0e-8390.66probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103504532 P... [more]
Match NameE-valueIdentityDescription
AT1G24620.12.8e-5761.54EF hand calcium-binding protein family [more]
AT1G66400.12.3e-3545.70calmodulin like 23 [more]
AT5G37770.15.1e-3547.92EF hand calcium-binding protein family [more]
AT1G18210.19.0e-3245.00Calcium-binding EF-hand family protein [more]
AT1G18210.29.0e-3245.00Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 36..64
e-value: 4.4E-6
score: 36.2
coord: 145..173
e-value: 8.1E-6
score: 35.3
coord: 109..137
e-value: 1.3E-5
score: 34.7
coord: 72..100
e-value: 2.2E-5
score: 33.9
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 36..95
e-value: 3.5E-14
score: 53.0
coord: 108..170
e-value: 3.2E-13
score: 49.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 105..140
score: 15.433954
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 32..67
score: 15.210791
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 68..103
score: 14.206556
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 141..176
score: 13.174425
IPR002048EF-hand domainCDDcd00051EFhcoord: 110..171
e-value: 6.36059E-20
score: 76.8177
IPR002048EF-hand domainCDDcd00051EFhcoord: 36..96
e-value: 1.29381E-19
score: 76.0473
NoneNo IPR availableGENE3D1.10.238.10coord: 100..176
e-value: 2.2E-25
score: 90.8
coord: 8..99
e-value: 1.2E-25
score: 91.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR23050:SF382CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 10..176
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 10..176
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 154..166
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 118..130
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 45..57
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 81..93
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 32..172

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10009567.1HG10009567.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding