HG10008169 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10008169
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRNase H domain-containing protein
LocationChr10: 20473751 .. 20474607 (+)
RNA-Seq ExpressionHG10008169
SyntenyHG10008169
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGAGTTTAAAAATAATACGTAGAGTGTTAGTCATATTCGTTCGAAATTTTCTCATATAATTTGGAGGTGAAGAAATGAATTTTGAGACAAGACCGGAGGGGAAGCAAAAATTCTGGAGTAAATAGTTCAGAAAGGAGGGTGAAATCTGGAGACTTTTATCTCCAATTCATTGTAAAAAATCCTTTTATTTGTTGTTCGGCTAACTTCCACATACCACTCTTTACTAATTTTGAAGGAATAACTTTTTTGTTCCAATGGCAATTGTGTTTAGAGAAACTCCCTTCGAGTTCTGCTTTGATTTTTTGTGGGGTAGATTTACTTCTGATTTGTGGTTGGTGTGGACACTTAGGAGAGAATCTACATTATATCCTTTGGCTCTGTAAAGTGGCTTGCAAGGAATGAGCTCGGTCTTCCTTTGCTCATATGTGGTGCAGTACTTCAAGAAAAACAATCTGTGTTCTTATGGAGATTCATGATCAACTGCTGGTTTTATAAACTCAATGTTGATGCAGGTTTCATTCCAGAAACAAATGAAGCCTCAGCGAGAATTATCATTCGAGATAATGAAGGGCGGGTCATGCTACCTGCTTTCAAAAGTTTAGGTCCTCTTTCTCATATGGACTTGGCCGAGGGATGTGCAGTGCAGACTGGGATCTCCCTATCTCTTGAAATGGATTTTCGATCTTTGATTGTGGAAACTGATTCGTTGCAGGTTCATCATTTGTTATATTCTGAGGTGGAAGACATTTATGAACTAGGTCGTTTATTCTTTACTTTAAAGAATGGTTTGTTGCGCTCCTGTGTATATTTCTTCTCTCTCCTCACTCTGGAAAGAGAGCTGCTCATTCGTTGA

mRNA sequence

ATGGAGAGAATCTACATTATATCCTTTGGCTCTGTAAAGTGGCTTGCAAGGAATGAGCTCGGTCTTCCTTTGCTCATATGTGGTGCAGTACTTCAAGAAAAACAATCTGTGTTCTTATGGAGATTCATGATCAACTGCTGGTTTTATAAACTCAATGTTGATGCAGGTTTCATTCCAGAAACAAATGAAGCCTCAGCGAGAATTATCATTCGAGATAATGAAGGGCGGGTCATGCTACCTGCTTTCAAAAGTTTAGGTCCTCTTTCTCATATGGACTTGGCCGAGGGATGTGCAGTGCAGACTGGGATCTCCCTATCTCTTGAAATGGATTTTCGATCTTTGATTGTGGAAACTGATTCGTTGCAGGTTCATCATTTGTTATATTCTGAGGTGGAAGACATTTATGAACTAGGTCGTTTATTCTTTACTTTAAAGAATGGTTTGTTGCGCTCCTGTGTATATTTCTTCTCTCTCCTCACTCTGGAAAGAGAGCTGCTCATTCGTTGA

Coding sequence (CDS)

ATGGAGAGAATCTACATTATATCCTTTGGCTCTGTAAAGTGGCTTGCAAGGAATGAGCTCGGTCTTCCTTTGCTCATATGTGGTGCAGTACTTCAAGAAAAACAATCTGTGTTCTTATGGAGATTCATGATCAACTGCTGGTTTTATAAACTCAATGTTGATGCAGGTTTCATTCCAGAAACAAATGAAGCCTCAGCGAGAATTATCATTCGAGATAATGAAGGGCGGGTCATGCTACCTGCTTTCAAAAGTTTAGGTCCTCTTTCTCATATGGACTTGGCCGAGGGATGTGCAGTGCAGACTGGGATCTCCCTATCTCTTGAAATGGATTTTCGATCTTTGATTGTGGAAACTGATTCGTTGCAGGTTCATCATTTGTTATATTCTGAGGTGGAAGACATTTATGAACTAGGTCGTTTATTCTTTACTTTAAAGAATGGTTTGTTGCGCTCCTGTGTATATTTCTTCTCTCTCCTCACTCTGGAAAGAGAGCTGCTCATTCGTTGA

Protein sequence

MERIYIISFGSVKWLARNELGLPLLICGAVLQEKQSVFLWRFMINCWFYKLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSLEMDFRSLIVETDSLQVHHLLYSEVEDIYELGRLFFTLKNGLLRSCVYFFSLLTLERELLIR
Homology
BLAST of HG10008169 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 68.2 bits (165), Expect = 7.9e-08
Identity = 40/96 (41.67%), Postives = 56/96 (58.33%), Query Frame = 0

Query: 50  KLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSLEM 109
           K+NVDA F  E+  A   +IIRD+ G V L A + L   S +D  EG AV  GI L++E 
Sbjct: 113 KVNVDAAFRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEA 172

Query: 110 DFRSLIVETDSLQVHHLLYSEVEDIYELGRLFFTLK 146
            F    +ETDSL++ +LL ++  D  E+G L   +K
Sbjct: 173 GFIRFQIETDSLRIFNLLTTDCVDDSEVGVLCSVIK 208

BLAST of HG10008169 vs. NCBI nr
Match: EEE59920.1 (hypothetical protein OsJ_12548 [Oryza sativa Japonica Group])

HSP 1 Score: 65.1 bits (157), Expect = 6.7e-07
Identity = 35/74 (47.30%), Postives = 47/74 (63.51%), Query Frame = 0

Query: 50  KLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSLEM 109
           K+NVDAGF  ET EASA I+IRD  G ++L A K+L P S  + AE  A   GI  +L+ 
Sbjct: 921 KINVDAGFRMETGEASAGIVIRDCRGLILLAACKTLHPCSSAEQAEALASLEGIRCALQW 980

Query: 110 DFRSLIVETDSLQV 124
               +I+ETD+ +V
Sbjct: 981 IHMPVILETDNAEV 994

BLAST of HG10008169 vs. NCBI nr
Match: EEC76169.1 (hypothetical protein OsI_13484 [Oryza sativa Indica Group])

HSP 1 Score: 65.1 bits (157), Expect = 6.7e-07
Identity = 35/74 (47.30%), Postives = 47/74 (63.51%), Query Frame = 0

Query: 50   KLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSLEM 109
            K+NVDAGF  ET EASA I+IRD  G ++L A K+L P S  + AE  A   GI  +L+ 
Sbjct: 1115 KINVDAGFRMETGEASAGIVIRDCRGLILLAACKTLHPCSSAEQAEALASLEGIRCALQW 1174

Query: 110  DFRSLIVETDSLQV 124
                +I+ETD+ +V
Sbjct: 1175 IHMPVILETDNAEV 1188

BLAST of HG10008169 vs. NCBI nr
Match: XP_030946082.1 (uncharacterized protein LOC115970617 [Quercus lobata])

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-06
Identity = 39/107 (36.45%), Postives = 55/107 (51.40%), Query Frame = 0

Query: 50  KLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSLEM 109
           K+N D     ET++A   +++RD++GRVM    K     S  D  E  AV   IS +LE+
Sbjct: 22  KINFDGTIFHETDKAGLGVVVRDHQGRVMASMSKKTKLPSSSDEVEALAVVRAISFALEL 81

Query: 110 DFRSLIVETDSLQVHHLLYSEVEDIYELGRLFFTLKNGL-LRSCVYF 156
              S+IVE DS  +   L SE E     G L  ++K  L + SC+ F
Sbjct: 82  HLPSVIVEGDSELIISALRSEEESFTSFGHLISSIKQSLGIFSCISF 128

BLAST of HG10008169 vs. NCBI nr
Match: XP_022135942.1 (uncharacterized protein LOC111007775 [Momordica charantia])

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-06
Identity = 37/99 (37.37%), Postives = 57/99 (57.58%), Query Frame = 0

Query: 48  FYKLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSL 107
           F+K+NVDA F      A   I+IRD+   V+L A   +  +S + LAE  A Q G+ L++
Sbjct: 80  FFKVNVDAAFKKGNFSAGLDILIRDSTVNVLLSAMCFITHVSDVTLAECLAAQEGVCLAI 139

Query: 108 EMDFRSLIVETDSLQVHHLLYSEVEDIYELGRLFFTLKN 147
           E       +ETDS QV +LL ++ ED  E+G L  ++++
Sbjct: 140 EAGLIPFQIETDSSQVFNLLRTDCEDESEIGVLASSIRH 178

BLAST of HG10008169 vs. ExPASy TrEMBL
Match: A0A6J1DBJ7 (uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018973 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.8e-08
Identity = 40/96 (41.67%), Postives = 56/96 (58.33%), Query Frame = 0

Query: 50  KLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSLEM 109
           K+NVDA F  E+  A   +IIRD+ G V L A + L   S +D  EG AV  GI L++E 
Sbjct: 113 KVNVDAAFRKESFVAGVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEA 172

Query: 110 DFRSLIVETDSLQVHHLLYSEVEDIYELGRLFFTLK 146
            F    +ETDSL++ +LL ++  D  E+G L   +K
Sbjct: 173 GFIRFQIETDSLRIFNLLTTDCVDDSEVGVLCSVIK 208

BLAST of HG10008169 vs. ExPASy TrEMBL
Match: B8AJV1 (Uncharacterized protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_13484 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.2e-07
Identity = 35/74 (47.30%), Postives = 47/74 (63.51%), Query Frame = 0

Query: 50   KLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSLEM 109
            K+NVDAGF  ET EASA I+IRD  G ++L A K+L P S  + AE  A   GI  +L+ 
Sbjct: 1115 KINVDAGFRMETGEASAGIVIRDCRGLILLAACKTLHPCSSAEQAEALASLEGIRCALQW 1174

Query: 110  DFRSLIVETDSLQV 124
                +I+ETD+ +V
Sbjct: 1175 IHMPVILETDNAEV 1188

BLAST of HG10008169 vs. ExPASy TrEMBL
Match: B9FBS8 (Uncharacterized protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_12548 PE=4 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.2e-07
Identity = 35/74 (47.30%), Postives = 47/74 (63.51%), Query Frame = 0

Query: 50  KLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSLEM 109
           K+NVDAGF  ET EASA I+IRD  G ++L A K+L P S  + AE  A   GI  +L+ 
Sbjct: 921 KINVDAGFRMETGEASAGIVIRDCRGLILLAACKTLHPCSSAEQAEALASLEGIRCALQW 980

Query: 110 DFRSLIVETDSLQV 124
               +I+ETD+ +V
Sbjct: 981 IHMPVILETDNAEV 994

BLAST of HG10008169 vs. ExPASy TrEMBL
Match: A0A6J1C467 (uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007775 PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 9.4e-07
Identity = 37/99 (37.37%), Postives = 57/99 (57.58%), Query Frame = 0

Query: 48  FYKLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSL 107
           F+K+NVDA F      A   I+IRD+   V+L A   +  +S + LAE  A Q G+ L++
Sbjct: 80  FFKVNVDAAFKKGNFSAGLDILIRDSTVNVLLSAMCFITHVSDVTLAECLAAQEGVCLAI 139

Query: 108 EMDFRSLIVETDSLQVHHLLYSEVEDIYELGRLFFTLKN 147
           E       +ETDS QV +LL ++ ED  E+G L  ++++
Sbjct: 140 EAGLIPFQIETDSSQVFNLLRTDCEDESEIGVLASSIRH 178

BLAST of HG10008169 vs. ExPASy TrEMBL
Match: A0A5B7BLL9 (RNase H domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_039108 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.4e-05
Identity = 33/102 (32.35%), Postives = 54/102 (52.94%), Query Frame = 0

Query: 49  YKLNVDAGFIPETNEASARIIIRDNEGRVMLPAFKSLGPLSHMDLAEGCAVQTGISLSLE 108
           YK+NVD  F+PE N  S   +IR+ +G V+    + L   S ++ AE  A+  GI  + +
Sbjct: 12  YKVNVDGSFVPEINHGSTCGVIRNFKGEVIAGFARPLPHCSSVEYAEAMALLHGIDFAFD 71

Query: 109 MDFRSLIVETDSLQVHHLLYSEVEDIYELGRLFFTLKNGLLR 151
           +  + L+VE D L +   + S  ED   +G +   +K G +R
Sbjct: 72  IGIQDLVVEGDCLHLISEISSSHEDFSVVGHIVDEIKMGSVR 113

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150944.17.9e-0841.67uncharacterized protein LOC111018973 [Momordica charantia][more]
EEE59920.16.7e-0747.30hypothetical protein OsJ_12548 [Oryza sativa Japonica Group][more]
EEC76169.16.7e-0747.30hypothetical protein OsI_13484 [Oryza sativa Indica Group][more]
XP_030946082.11.9e-0636.45uncharacterized protein LOC115970617 [Quercus lobata][more]
XP_022135942.11.9e-0637.37uncharacterized protein LOC111007775 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DBJ73.8e-0841.67uncharacterized protein LOC111018973 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
B8AJV13.2e-0747.30Uncharacterized protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_13484 PE=3... [more]
B9FBS83.2e-0747.30Uncharacterized protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_12548 PE... [more]
A0A6J1C4679.4e-0737.37uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007... [more]
A0A5B7BLL91.4e-0532.35RNase H domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_039108 ... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 52..132
e-value: 5.8E-11
score: 42.3
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 49..146
e-value: 5.3E-5
score: 25.3
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 49..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10008169.1HG10008169.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity