HG10003802 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10003802
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionBiotin synthase
LocationChr08: 9345694 .. 9350503 (+)
RNA-Seq ExpressionHG10003802
SyntenyHG10003802
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGGTTTTAAGGCTGAGCATGTCAATGGTAACATCTCAAACAGTTCAGGTTTTGTTTCTTTTTACTTGACGTTCTAAAACCTTGGCAGAATCACCTTTGAAATATTATGGAATATTTATGATTATTGACTATGTTTCTTTAGTTTCTCTCGTTGAATCTAGCTTTAGTTTATGTACTTAGGATGCCTTAAAAAGTGACACTACACATGCAATATTGGATTATTACTTCATCCATCTTGCATTTGGAAAATTGTTGCCTCTAAGGGGATTTCTCCAAGGTGATCCAGCTCCCCTTTATTTTCACTACCATGGTGGACTCACGAAATGGATATTTTTCTAAGCCATTTCCTGTAGGTAGATGAGAAGGCTTGATATTAGGTAGAGACCTTTTTATTATCTCTCATTTACAATTTGTAGATGACACCCTGCTTTTAATTGAGGAAGAGACTAAGCCTTTATTTGCTTTTGACTTGTTGGGAACATTGAGTTGGCCTTTAGTCTGAGGTCATCATGTCTAAACTATGTTTTTTCATCTTCCTGATGTTAACAATATTTTGTTTAAGAGCTCTGCAGATTAGAATTGTGGTGGAAATGTCCTTTAAAGGTTAAAGTAGTCGGTCTCTTCTCCTTAAATCTATTACCATTGCTGCTAGAATTAAAAAGTGAACCCCTTACCACAATTTTTAACCTCAAATTTGTTGGTAGTCTGTTAAAGAGGACTTGAACCCTATACTCATCCACTACCCTTTTGCTTATGGATGTTGATTCTAGTGGGGTTCTCCCTTGAGGCTTCAGTAATATTGGCCTAAGTATATGTTGGCTCATGAGCAGCAAGCTCTTGGGTACCAAGGCACTGGTACTTCGAGATTATGCTTTCAACGTTCTCTATCAAGTCAAAAGAATGCTCGGATTGATGAAGATATATAGAAAATGTCACCCTCCCACCTCATATTGCTCTTTCGACTGGACTTTCTTATAACTATTCTTCTAACTATCATAACTACCTATTTAGAAGCTTTTCTGCTATTCTAGTGGCTTGTGGCTTGGCGATAGGTTATCTCCTCCATCCTTTTGTATAGTCTTTTGTTTCTCAGGATATTTAGGTTTTCAAATTATTTTGGCGAGATCCTCTTACAAAATTTCGAGTGTCTCTTAGTGAGATGAGTAGAAAAGGAATCGGGAATAAAATCCATGAACTGTTAAAGAGTCCAACATATACATTAGGCCATTGATGAGAATGTAAATTCATTTAAATGTCCTTAAAAAGTAACTTCCCTTACTTCTCCTGAACTATCCTAGTTGCATCAAAGATAACCACTCCTAGAAAACTTTTCTGAGAATTGGAACTATGTTAGTCTATAGATGATTTTTCACTTAATTTGGGTCTTCTGCAATTCCTTCAAAAATAATGTCCATCAGGTCCTTTATGGAGTGTCTTTGAACCCTGTTTCCCAACAGTTATGGATTAATGCAATCAAAGCACTTCTTGTTGAATTATGGTTTGAAAGAAATCAGATAGTTTTCCATGAAAAGCATCTTTAGTGGTCCGATCGTTTTGAAGTAGCAAGACTCAACGCTTCTGCTTGGTGTTCTCTATCTAAGTCTTTTACGAATTTTTCCATTCAAGATATTTGTCTGAACTGGCGAGCTTTAATTTTTTTCAGCTTGATCTTTGTCTTTGCATCATACTGTTAGATGCTTTTGTGCTATTATTGTTTTTTCTTTTCATACTCTTGCTTACTTTGTTTCATTGTATTTTTTAGCCTTAGACTCTTTTCATTATAATAATAATTTATTAATGGAAACAGATTCATTAGACAAATGGTGTTACAATTTTTTTTTCTTTTTTATTTTCCCATAATGCAACTTATCCTCCTTTTCAATCGTCAAGTTCTCAAATGGGTTTATTTCACTTGAAGCAATAGCTTCATGTTTTTTTCCCTATTAATTTTGTTTGCTAACATATATATACTTCTTCAGGCACAAGTTCATAGGCACACACACAATTTCCGGGAGGTGCAGCAGTGTACTCTCCTTTCAATCAAAACAGGTGGATGTAGTGAGGATTGTTCCTATTGTCCTCAATCATCGAGATATGACACTGGAGTGAAAGCTCAAAAGCTTATGACCAAGGATGTTGTCCTGGAGGCAGCCAAAAAGGTATCCCTTTTAATTGGGGCAAGTTGTTCTGTACATTCGAGCTGAGTCTCATTATAGAGACTAACCAATAATAAAGTTATATATGTCTAAGATAAGATGTGCATTTACATTTTACTAGAGCATAAAATCAAATGCACTTCTGATCTATAAAATAATTAGAAGCAATTTTTTTTTAATTATTTTTTATTCTAAAATTATCATCAATCTGACAACCGTCTGGGCAGCTTAGCATTCTTCTTCAAGAATACTTGATGACTTACATGTTCGGTATTTATGATAACTTGGTATGAAAAATATTTTATTGAACAAGGCTTATATTACAAAATAAATTATTTGTTTTTTTATTTAGAACAACATAATGTGCATTGCGCTTTCCACGAAATCATGGCATGCTGATTGGTAGACTTTTAATAACTTTCTTTCTACTTGTATCTTTCATATAAAAAATTATAACATGTCTATATCTGAACTTTTCCTCTTTTATTTCTTCACTCCATGCTATCCTCTTTCTTAAAAGGAGGTTGAAAAATGTTAAAAAGGAATGATTTTTTTATTGCATTTTATCTATCCCTTGAGCACCATTTAAAGCAGATATTCACATTGTCTGGCAGGCAAAAGAGGCTGGTAGCACAAGATTTTGTATGGGTGCTGCTTGGAGAGACACAATAGGAAGAAAAACTAATTTCAATCAGATCCTTGATTATGTAAAAGAAATAAGGTAACGAAATTTCACCTTGAATAAAAAATAGCTACAAAGATAAGAAGACTAAAATAAGTAGTTTACTTACAGTATACAGGATAAAACTGCTGTTGTATCTTATAGTGAATCATGGCGCAGAGCAGAGCACTTAACAAGAATCTGCAACGATTCAAAAAACACATTCAGTCAACGATTTGAAAGTGCCTAGTGTTGCACTTGTGTTCCCCCACCCGCTTTTATTTTCTTTTCATACTAAGAAGGAAGTTGCTAGGATATTTCTACTAAGCACAACCTCTTAATCAAAGCCTTTTTCTTTTCTCATTTTTCTCATTCTCTCCTCAAAGCTCGGAGTTTGGTTTGTATATATATAAGACTTCTGCAATTGTTAAGATTAGCTAGATTTGACTCTTTAATATTGTCCAATAAAGCTTGTGAATTCATGATTGAGAAACTGAAACTTTGGCGAGAATCTACCACAATTATAGCTCTTTTGACTTGAGTAATCACTTGTCCATCGATATTTGTTCTCAAATCAAATTTACCTGTGTTGTATTAGAGAGATGGGGATGGAGGTCTGCTGCACCCTGGGCATGATAGAGAAGCAACAAGCTATTGACCTCAAAAAGGCAGGCCTAACAGCTTATAACCACAACCTGGACACGTCAAGGGAATACTACCCAAACATTATTACCACAAGGAGTTATGATGAGCGTTTGAAAACCCTAGAATTTGTTCGTGATGCTGGAATCAATGTCTGCTCAGGTAATTTTGTACATCTTTGATTTTGGCTCTGATTTGGATTGATCTATTGACAATTAGATTGGATATTCCTAGATTCATAACTTATTTATAGGGAAAACTTCCATGAATGAGTAACTACTAGAAGATATGGTTTTTGAAAGAAGGAAAAGCCCCCACAAGATCACGAGACAACGAAAGGGCTCTCCAGTTGGCATTTGGCAATTATTTAGGATTCATAACACATGTAAGGAAAAATATATTCCTGGAAAATGCTCAGCCTGGTCAGAAAACCAAGAAGTCTACTAAAAGTTATAATTATTGACAATTTGAGAGGATAATATTCATTTCAGTGTAAAGTAGATATGGCCTTTAACATGGTTAGGGGTTTCATTGATTAGTAGACCAACTATAGTGATTTTTTTCAACTCCTTTGGCTACCAGTAATGACTTCCTTTGAAAGCTAAAGGTTTATCTTTAATTTTAGGCTTGTGAATTTTTGGTGTATTTTGTCCCGAATAAATACGGGTTTGGTATTTTCACTGCTTAATAATCTTATAACTTGGTGAAATTCTTAATTTCTTGCAATAGGAGGAATAATTGGGCTTGGCGAGGCAGAAGAGGACCGTGTCGGTTTGCTGCATACCTTGGCGACACTGCCGACACACCCTGAGAGTGTTCCCATCAACGCACTGATTGCAGTGAAAGGAACCCCTCTTCAAGATCAAAAAGTAGCTTCAATTTACTCAAAATATTAACCATTTTGTTTATTGCTCTTGAAGTCTAGTTTACACTCTTGTTCATAACTGTTGCACATACCTTATTTCGACATGACAACTTTATTACTCATGAAAGCTACTGCTTTGGTTTCTATAGTGAATAGTTTTCTTGCCTTTCTGTTCAGCCTGTTGAAATATGGGAGATGATTCGGATGATCGCTACAGCACGGATCACGATGCCTAAAGCAATGGTAAGGTTGTCAGCAGGTAGAGTGAAGTTCTCCGTGCCCGAGCAGGCGTTATGCTTCCTCGCAGGAGCAAATTCTATCTTCACTGGTGAGAAACTACTAACGACACCAAATAATGATTTCGATGCTGATCAACTAATGTTCAAGATGCTGGGTCTGATTCCAAAAGCTCCCAGTTTCACTGAAGATGTTGAGAAAGCACCTCAAGAAGAGTCATTACAAGAAGCTGCTTCCAGTGCATAA

mRNA sequence

ATGAGGGTTTTAAGGCTGAGCATGTCAATGGTAACATCTCAAACAGTTCAGGCACAAGTTCATAGGCACACACACAATTTCCGGGAGGTGCAGCAGTGTACTCTCCTTTCAATCAAAACAGGTGGATGTAGTGAGGATTGTTCCTATTGTCCTCAATCATCGAGATATGACACTGGAGTGAAAGCTCAAAAGCTTATGACCAAGGATGTTGTCCTGGAGGCAGCCAAAAAGGCAAAAGAGGCTGGTAGCACAAGATTTTGTATGGGTGCTGCTTGGAGAGACACAATAGGAAGAAAAACTAATTTCAATCAGATCCTTGATTATGTAAAAGAAATAAGAGAGATGGGGATGGAGGTCTGCTGCACCCTGGGCATGATAGAGAAGCAACAAGCTATTGACCTCAAAAAGGCAGGCCTAACAGCTTATAACCACAACCTGGACACGTCAAGGGAATACTACCCAAACATTATTACCACAAGGAGTTATGATGAGCGTTTGAAAACCCTAGAATTTGTTCGTGATGCTGGAATCAATGTCTGCTCAGGAGGAATAATTGGGCTTGGCGAGGCAGAAGAGGACCGTGTCGGTTTGCTGCATACCTTGGCGACACTGCCGACACACCCTGAGAGTGTTCCCATCAACGCACTGATTGCAGTGAAAGGAACCCCTCTTCAAGATCAAAAACCTGTTGAAATATGGGAGATGATTCGGATGATCGCTACAGCACGGATCACGATGCCTAAAGCAATGGTAAGGTTGTCAGCAGGTAGAGTGAAGTTCTCCGTGCCCGAGCAGGCGTTATGCTTCCTCGCAGGAGCAAATTCTATCTTCACTGGTGAGAAACTACTAACGACACCAAATAATGATTTCGATGCTGATCAACTAATGTTCAAGATGCTGGGTCTGATTCCAAAAGCTCCCAGTTTCACTGAAGATGTTGAGAAAGCACCTCAAGAAGAGTCATTACAAGAAGCTGCTTCCAGTGCATAA

Coding sequence (CDS)

ATGAGGGTTTTAAGGCTGAGCATGTCAATGGTAACATCTCAAACAGTTCAGGCACAAGTTCATAGGCACACACACAATTTCCGGGAGGTGCAGCAGTGTACTCTCCTTTCAATCAAAACAGGTGGATGTAGTGAGGATTGTTCCTATTGTCCTCAATCATCGAGATATGACACTGGAGTGAAAGCTCAAAAGCTTATGACCAAGGATGTTGTCCTGGAGGCAGCCAAAAAGGCAAAAGAGGCTGGTAGCACAAGATTTTGTATGGGTGCTGCTTGGAGAGACACAATAGGAAGAAAAACTAATTTCAATCAGATCCTTGATTATGTAAAAGAAATAAGAGAGATGGGGATGGAGGTCTGCTGCACCCTGGGCATGATAGAGAAGCAACAAGCTATTGACCTCAAAAAGGCAGGCCTAACAGCTTATAACCACAACCTGGACACGTCAAGGGAATACTACCCAAACATTATTACCACAAGGAGTTATGATGAGCGTTTGAAAACCCTAGAATTTGTTCGTGATGCTGGAATCAATGTCTGCTCAGGAGGAATAATTGGGCTTGGCGAGGCAGAAGAGGACCGTGTCGGTTTGCTGCATACCTTGGCGACACTGCCGACACACCCTGAGAGTGTTCCCATCAACGCACTGATTGCAGTGAAAGGAACCCCTCTTCAAGATCAAAAACCTGTTGAAATATGGGAGATGATTCGGATGATCGCTACAGCACGGATCACGATGCCTAAAGCAATGGTAAGGTTGTCAGCAGGTAGAGTGAAGTTCTCCGTGCCCGAGCAGGCGTTATGCTTCCTCGCAGGAGCAAATTCTATCTTCACTGGTGAGAAACTACTAACGACACCAAATAATGATTTCGATGCTGATCAACTAATGTTCAAGATGCTGGGTCTGATTCCAAAAGCTCCCAGTTTCACTGAAGATGTTGAGAAAGCACCTCAAGAAGAGTCATTACAAGAAGCTGCTTCCAGTGCATAA

Protein sequence

MRVLRLSMSMVTSQTVQAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKKAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKAGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGLLHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAPQEESLQEAASSA
Homology
BLAST of HG10003802 vs. NCBI nr
Match: XP_008445703.1 (PREDICTED: biotin synthase [Cucumis melo])

HSP 1 Score: 614.8 bits (1584), Expect = 4.4e-172
Identity = 306/310 (98.71%), Postives = 308/310 (99.35%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           AQVHRH HNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK
Sbjct: 74  AQVHRHAHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 133

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLATLPTHPESVPINAL+AVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTED EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKAP 373

Query: 318 QEESLQEAAS 328
           +EESLQEAAS
Sbjct: 374 EEESLQEAAS 383

BLAST of HG10003802 vs. NCBI nr
Match: XP_038886510.1 (biotin synthase, mitochondrial [Benincasa hispida])

HSP 1 Score: 614.8 bits (1584), Expect = 4.4e-172
Identity = 306/312 (98.08%), Postives = 310/312 (99.36%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLM KDVVLEAAKK
Sbjct: 74  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMIKDVVLEAAKK 133

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLATLPTHPESVPINAL+AVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDV+KAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVQKAP 373

Query: 318 QEESLQEAASSA 330
           +EESLQE ASS+
Sbjct: 374 EEESLQEVASSS 385

BLAST of HG10003802 vs. NCBI nr
Match: XP_004140874.1 (biotin synthase, mitochondrial [Cucumis sativus] >KGN45987.1 hypothetical protein Csa_005313 [Cucumis sativus])

HSP 1 Score: 614.8 bits (1584), Expect = 4.4e-172
Identity = 306/312 (98.08%), Postives = 310/312 (99.36%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTG+KAQKLMTKDVVLEAAKK
Sbjct: 74  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGLKAQKLMTKDVVLEAAKK 133

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLATLPTHPESVPINAL+AVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTED EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKAP 373

Query: 318 QEESLQEAASSA 330
           +EESLQEAA S+
Sbjct: 374 EEESLQEAALSS 385

BLAST of HG10003802 vs. NCBI nr
Match: KAA0033098.1 (biotin synthase [Cucumis melo var. makuwa] >TYK17135.1 biotin synthase [Cucumis melo var. makuwa])

HSP 1 Score: 614.8 bits (1584), Expect = 4.4e-172
Identity = 306/311 (98.39%), Postives = 309/311 (99.36%), Query Frame = 0

Query: 17  QAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAK 76
           +AQVHRH HNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAK
Sbjct: 79  RAQVHRHAHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAK 138

Query: 77  KAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKK 136
           KAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKK
Sbjct: 139 KAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKK 198

Query: 137 AGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVG 196
           AGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVG
Sbjct: 199 AGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVG 258

Query: 197 LLHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAG 256
           LLHTLATLPTHPESVPINAL+AVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAG
Sbjct: 259 LLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAG 318

Query: 257 RVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKA 316
           RVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTED EKA
Sbjct: 319 RVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKA 378

Query: 317 PQEESLQEAAS 328
           P+EESLQEAAS
Sbjct: 379 PEEESLQEAAS 389

BLAST of HG10003802 vs. NCBI nr
Match: XP_023549140.1 (biotin synthase, mitochondrial [Cucurbita pepo subsp. pepo] >KAG6575351.1 Biotin synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013886.1 Biotin synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 609.0 bits (1569), Expect = 2.4e-170
Identity = 303/311 (97.43%), Postives = 308/311 (99.04%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVK+QKLMTKDVVLEAAKK
Sbjct: 74  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQKLMTKDVVLEAAKK 133

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLATLPTHPESVPINAL+AVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQ MFKMLGLIPKAPSFT+  EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIPKAPSFTDGAEKAP 373

Query: 318 QEESLQEAASS 329
           +EESLQEAAS+
Sbjct: 374 EEESLQEAASN 384

BLAST of HG10003802 vs. ExPASy Swiss-Prot
Match: P54967 (Biotin synthase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BIO2 PE=1 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 3.0e-155
Identity = 267/309 (86.41%), Postives = 290/309 (93.85%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           AQVHRH HNFREVQQCTLLSIKTGGCSEDCSYCPQSSRY TGVKAQ+LM+KD V++AAKK
Sbjct: 69  AQVHRHVHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYSTGVKAQRLMSKDAVIDAAKK 128

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKEAGSTRFCMGAAWRDTIGRKTNF+QIL+Y+KEIR MGMEVCCTLGMIEKQQA++LKKA
Sbjct: 129 AKEAGSTRFCMGAAWRDTIGRKTNFSQILEYIKEIRGMGMEVCCTLGMIEKQQALELKKA 188

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYYPN+ITTRSYD+RL+TL  VRDAGINVCSGGIIGLGEAEEDR+GL
Sbjct: 189 GLTAYNHNLDTSREYYPNVITTRSYDDRLETLSHVRDAGINVCSGGIIGLGEAEEDRIGL 248

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLATLP+HPESVPINAL+AVKGTPL+DQKPVEIWEMIRMI TARI MPKAMVRLSAGR
Sbjct: 249 LHTLATLPSHPESVPINALLAVKGTPLEDQKPVEIWEMIRMIGTARIVMPKAMVRLSAGR 308

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           V+FS+ EQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFK LGLIPK PSF+ED  ++ 
Sbjct: 309 VRFSMSEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKTLGLIPKPPSFSEDDSESE 368

Query: 318 QEESLQEAA 327
             E +  A+
Sbjct: 369 NCEKVASAS 377

BLAST of HG10003802 vs. ExPASy Swiss-Prot
Match: Q11S94 (Biotin synthase OS=Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465) OX=269798 GN=bioB PE=3 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.5e-106
Identity = 193/303 (63.70%), Postives = 238/303 (78.55%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           A VHR  H+ +EVQ CTLLSIKTGGC EDCSYCPQ++RY T VK +KLM    VL +A +
Sbjct: 29  ATVHREFHDPQEVQVCTLLSIKTGGCPEDCSYCPQAARYHTDVKVEKLMDVKDVLNSALE 88

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKE+GSTRFCMGAAWR+    K +F++++D VK +  MGMEVCCTLGM+  +QA  LK A
Sbjct: 89  AKESGSTRFCMGAAWREVRDNK-DFDKVIDMVKGVSTMGMEVCCTLGMLTPEQADKLKDA 148

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GL AYNHNLDTS E+Y  +ITTR+YD+RL+TL+ VR+A I+VCSGGIIG+GE+  DRVG+
Sbjct: 149 GLYAYNHNLDTSAEHYDKVITTRTYDDRLETLDNVRNAKISVCSGGIIGMGESHGDRVGM 208

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLA +  HPESVP+NAL+ V+GTPL+DQ  V +WEM+RMIATARI MPKAMVRLSAGR
Sbjct: 209 LHTLANMVEHPESVPVNALVPVEGTPLEDQPRVSVWEMVRMIATARIIMPKAMVRLSAGR 268

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           V+ +  EQALCFLAGANSIF G+KLLTTPN + +AD+ MF++L L P+      D  K  
Sbjct: 269 VRMNTEEQALCFLAGANSIFAGDKLLTTPNPEVNADKEMFQVLNLKPRQSFKNGDAPKIK 328

Query: 318 QEE 321
            E+
Sbjct: 329 FEQ 330

BLAST of HG10003802 vs. ExPASy Swiss-Prot
Match: Q5AYI7 (Biotin synthase, mitochondrial OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bioB PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.1e-104
Identity = 185/309 (59.87%), Postives = 232/309 (75.08%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           A VHR  H+   +Q CTL++IKTGGCSEDCSYC QSSRY TG+KA K+   D VLE A+ 
Sbjct: 71  AAVHRRFHDPSAIQMCTLMNIKTGGCSEDCSYCAQSSRYSTGLKATKMSPVDDVLEKARI 130

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AK  GSTRFCMGAAWRD  GRKT+   +   V  +REMGMEVC TLGMI+  QA +LK A
Sbjct: 131 AKANGSTRFCMGAAWRDMRGRKTSLKNVKQMVSGVREMGMEVCVTLGMIDADQAKELKDA 190

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSRE+YP IITTRSYDERLKTL  VRDAGINVCSGGI+GLGEA+ DR+GL
Sbjct: 191 GLTAYNHNLDTSREFYPTIITTRSYDERLKTLSHVRDAGINVCSGGILGLGEADSDRIGL 250

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           +HT+++LP+HPES P+NAL+ +KGTPL D+K +   +++R +ATARI +P  +VRL+AGR
Sbjct: 251 IHTVSSLPSHPESFPVNALVPIKGTPLGDRKMISFDKLLRTVATARIVLPATIVRLAAGR 310

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           +  +  +Q  CF+AGAN++FTGEK+LTT  N +D D+ MF   G  P   SF ++   A 
Sbjct: 311 ISLTEEQQVACFMAGANAVFTGEKMLTTDCNGWDEDRAMFDRWGFYPMR-SFEKETNAAT 370

Query: 318 QEESLQEAA 327
            ++ +   A
Sbjct: 371 PQQHVDSVA 378

BLAST of HG10003802 vs. ExPASy Swiss-Prot
Match: O59778 (Biotin synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bio2 PE=1 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.0e-99
Identity = 180/287 (62.72%), Postives = 221/287 (77.00%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           A +HR  H+ ++VQQCTLLSIKTGGC+EDC YC QSSRY+TGVKA KLM  D VLE AK 
Sbjct: 44  ASIHRKFHDPKKVQQCTLLSIKTGGCTEDCKYCAQSSRYNTGVKATKLMKIDEVLEKAKI 103

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AK  GSTRFCMG+AWRD  GR   F  IL+ +KE+R M MEVC TLGM+ +QQA +LK A
Sbjct: 104 AKAKGSTRFCMGSAWRDLNGRNRTFKNILEIIKEVRSMDMEVCVTLGMLNEQQAKELKDA 163

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYY  II+TR+YDERL T++ +R AG+ VCSGGI+GLGE + DRVGL
Sbjct: 164 GLTAYNHNLDTSREYYSKIISTRTYDERLNTIDNLRKAGLKVCSGGILGLGEKKHDRVGL 223

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQD--QKPVEIWEMIRMIATARITMPKAMVRLSA 257
           +H+LAT+PTHPESVP N L+ + GTP+ D  ++ + I   +R IATARI MPK ++R +A
Sbjct: 224 IHSLATMPTHPESVPFNLLVPIPGTPVGDAVKERLPIHPFLRSIATARICMPKTIIRFAA 283

Query: 258 GRVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGL 303
           GR   S  EQAL F+AGAN++FTGEK+LTTP   +D+D  +F   GL
Sbjct: 284 GRNTCSESEQALAFMAGANAVFTGEKMLTTPAVSWDSDSQLFYNWGL 330

BLAST of HG10003802 vs. ExPASy Swiss-Prot
Match: A5FLT1 (Biotin synthase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101) OX=376686 GN=bioB PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.9e-94
Identity = 169/290 (58.28%), Postives = 216/290 (74.48%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           A +HR  H+   VQ  TLLSIKTGGC EDC YCPQ++RY+TGV+   LMT   V   A +
Sbjct: 30  ATIHRQQHDPNVVQVSTLLSIKTGGCPEDCGYCPQAARYNTGVEGNDLMTVSQVKAQALR 89

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AK  GS+R CMGAAWR+ +     F+Q+L+ V+ I ++ MEVCCTLGM+ + QA  L +A
Sbjct: 90  AKSNGSSRVCMGAAWRN-VKDGEEFDQVLEMVRTINKLDMEVCCTLGMLTENQAQRLAEA 149

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GL AYNHNLDTS EYY ++I+TR +++RL+T+E VR   + VCSGGIIG+GE+ EDR G+
Sbjct: 150 GLYAYNHNLDTSEEYYKDVISTRGFEDRLQTIENVRKTNVTVCSGGIIGMGESIEDRAGM 209

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           L  L+TL   PESVPINAL+AV+GTP++++KPVEIWEMIRM+AT RI MP+  VRLSAGR
Sbjct: 210 LVALSTLNPQPESVPINALVAVEGTPMEEEKPVEIWEMIRMVATTRIVMPETQVRLSAGR 269

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAP 308
              S   QA+CF AGANSIF G+KLLTTPN D + D  MF+ LG++ + P
Sbjct: 270 TNMSREGQAMCFFAGANSIFAGDKLLTTPNPDVNEDMKMFETLGMVAQKP 318

BLAST of HG10003802 vs. ExPASy TrEMBL
Match: A0A5D3D025 (Biotin synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G00740 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.1e-172
Identity = 306/311 (98.39%), Postives = 309/311 (99.36%), Query Frame = 0

Query: 17  QAQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAK 76
           +AQVHRH HNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAK
Sbjct: 79  RAQVHRHAHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAK 138

Query: 77  KAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKK 136
           KAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKK
Sbjct: 139 KAKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKK 198

Query: 137 AGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVG 196
           AGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVG
Sbjct: 199 AGLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVG 258

Query: 197 LLHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAG 256
           LLHTLATLPTHPESVPINAL+AVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAG
Sbjct: 259 LLHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAG 318

Query: 257 RVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKA 316
           RVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTED EKA
Sbjct: 319 RVKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKA 378

Query: 317 PQEESLQEAAS 328
           P+EESLQEAAS
Sbjct: 379 PEEESLQEAAS 389

BLAST of HG10003802 vs. ExPASy TrEMBL
Match: A0A0A0KCA2 (Biotin synthase OS=Cucumis sativus OX=3659 GN=Csa_6G041720 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.1e-172
Identity = 306/312 (98.08%), Postives = 310/312 (99.36%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTG+KAQKLMTKDVVLEAAKK
Sbjct: 74  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGLKAQKLMTKDVVLEAAKK 133

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLATLPTHPESVPINAL+AVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTED EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKAP 373

Query: 318 QEESLQEAASSA 330
           +EESLQEAA S+
Sbjct: 374 EEESLQEAALSS 385

BLAST of HG10003802 vs. ExPASy TrEMBL
Match: A0A1S3BDC5 (Biotin synthase OS=Cucumis melo OX=3656 GN=LOC103488656 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.1e-172
Identity = 306/310 (98.71%), Postives = 308/310 (99.35%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           AQVHRH HNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK
Sbjct: 74  AQVHRHAHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 133

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLATLPTHPESVPINAL+AVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTED EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDAEKAP 373

Query: 318 QEESLQEAAS 328
           +EESLQEAAS
Sbjct: 374 EEESLQEAAS 383

BLAST of HG10003802 vs. ExPASy TrEMBL
Match: A0A6J1D9U9 (Biotin synthase OS=Momordica charantia OX=3673 GN=LOC111018547 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.4e-170
Identity = 302/312 (96.79%), Postives = 310/312 (99.36%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK
Sbjct: 74  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 133

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQA +LKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAEELKKA 193

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLATLP+HPESVPINAL+AVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPSHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSF++ VEKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFSDGVEKAP 373

Query: 318 QEESLQEAASSA 330
           +EESLQEAAS++
Sbjct: 374 EEESLQEAASNS 385

BLAST of HG10003802 vs. ExPASy TrEMBL
Match: A0A6J1EPY7 (Biotin synthase OS=Cucurbita moschata OX=3662 GN=LOC111436330 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.4e-170
Identity = 302/311 (97.11%), Postives = 308/311 (99.04%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVK+QKLMTKDVVLEAAKK
Sbjct: 74  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKSQKLMTKDVVLEAAKK 133

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVK+IREMGMEVCCTLGMIEKQQAIDLKKA
Sbjct: 134 AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKKIREMGMEVCCTLGMIEKQQAIDLKKA 193

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL
Sbjct: 194 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 253

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLATLPTHPESVPINAL+AVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR
Sbjct: 254 LHTLATLPTHPESVPINALVAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 313

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQ MFKMLGLIPKAPSFT+  EKAP
Sbjct: 314 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQHMFKMLGLIPKAPSFTDGAEKAP 373

Query: 318 QEESLQEAASS 329
           +EESLQEAAS+
Sbjct: 374 EEESLQEAASN 384

BLAST of HG10003802 vs. TAIR 10
Match: AT2G43360.1 (Radical SAM superfamily protein )

HSP 1 Score: 549.3 bits (1414), Expect = 2.1e-156
Identity = 267/309 (86.41%), Postives = 290/309 (93.85%), Query Frame = 0

Query: 18  AQVHRHTHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYDTGVKAQKLMTKDVVLEAAKK 77
           AQVHRH HNFREVQQCTLLSIKTGGCSEDCSYCPQSSRY TGVKAQ+LM+KD V++AAKK
Sbjct: 69  AQVHRHVHNFREVQQCTLLSIKTGGCSEDCSYCPQSSRYSTGVKAQRLMSKDAVIDAAKK 128

Query: 78  AKEAGSTRFCMGAAWRDTIGRKTNFNQILDYVKEIREMGMEVCCTLGMIEKQQAIDLKKA 137
           AKEAGSTRFCMGAAWRDTIGRKTNF+QIL+Y+KEIR MGMEVCCTLGMIEKQQA++LKKA
Sbjct: 129 AKEAGSTRFCMGAAWRDTIGRKTNFSQILEYIKEIRGMGMEVCCTLGMIEKQQALELKKA 188

Query: 138 GLTAYNHNLDTSREYYPNIITTRSYDERLKTLEFVRDAGINVCSGGIIGLGEAEEDRVGL 197
           GLTAYNHNLDTSREYYPN+ITTRSYD+RL+TL  VRDAGINVCSGGIIGLGEAEEDR+GL
Sbjct: 189 GLTAYNHNLDTSREYYPNVITTRSYDDRLETLSHVRDAGINVCSGGIIGLGEAEEDRIGL 248

Query: 198 LHTLATLPTHPESVPINALIAVKGTPLQDQKPVEIWEMIRMIATARITMPKAMVRLSAGR 257
           LHTLATLP+HPESVPINAL+AVKGTPL+DQKPVEIWEMIRMI TARI MPKAMVRLSAGR
Sbjct: 249 LHTLATLPSHPESVPINALLAVKGTPLEDQKPVEIWEMIRMIGTARIVMPKAMVRLSAGR 308

Query: 258 VKFSVPEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKMLGLIPKAPSFTEDVEKAP 317
           V+FS+ EQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFK LGLIPK PSF+ED  ++ 
Sbjct: 309 VRFSMSEQALCFLAGANSIFTGEKLLTTPNNDFDADQLMFKTLGLIPKPPSFSEDDSESE 368

Query: 318 QEESLQEAA 327
             E +  A+
Sbjct: 369 NCEKVASAS 377

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008445703.14.4e-17298.71PREDICTED: biotin synthase [Cucumis melo][more]
XP_038886510.14.4e-17298.08biotin synthase, mitochondrial [Benincasa hispida][more]
XP_004140874.14.4e-17298.08biotin synthase, mitochondrial [Cucumis sativus] >KGN45987.1 hypothetical protei... [more]
KAA0033098.14.4e-17298.39biotin synthase [Cucumis melo var. makuwa] >TYK17135.1 biotin synthase [Cucumis ... [more]
XP_023549140.12.4e-17097.43biotin synthase, mitochondrial [Cucurbita pepo subsp. pepo] >KAG6575351.1 Biotin... [more]
Match NameE-valueIdentityDescription
P549673.0e-15586.41Biotin synthase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BIO2 PE=1 SV=1[more]
Q11S941.5e-10663.70Biotin synthase OS=Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 10... [more]
Q5AYI71.1e-10459.87Biotin synthase, mitochondrial OS=Emericella nidulans (strain FGSC A4 / ATCC 381... [more]
O597781.0e-9962.72Biotin synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812... [more]
A5FLT12.9e-9458.28Biotin synthase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM... [more]
Match NameE-valueIdentityDescription
A0A5D3D0252.1e-17298.39Biotin synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G007... [more]
A0A0A0KCA22.1e-17298.08Biotin synthase OS=Cucumis sativus OX=3659 GN=Csa_6G041720 PE=3 SV=1[more]
A0A1S3BDC52.1e-17298.71Biotin synthase OS=Cucumis melo OX=3656 GN=LOC103488656 PE=3 SV=1[more]
A0A6J1D9U93.4e-17096.79Biotin synthase OS=Momordica charantia OX=3673 GN=LOC111018547 PE=3 SV=1[more]
A0A6J1EPY73.4e-17097.11Biotin synthase OS=Cucurbita moschata OX=3662 GN=LOC111436330 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G43360.12.1e-15686.41Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010722Biotin and thiamin synthesis-associated domainSMARTSM00876BATS_2coord: 212..304
e-value: 2.8E-40
score: 149.8
IPR010722Biotin and thiamin synthesis-associated domainPFAMPF06968BATScoord: 212..302
e-value: 2.5E-17
score: 62.8
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 33..241
e-value: 7.1E-42
score: 155.1
IPR024177Biotin synthasePIRSFPIRSF001619Biotin_synthcoord: 3..311
e-value: 4.4E-133
score: 441.5
IPR024177Biotin synthaseSFLDSFLDF00272biotin_synthasecoord: 18..309
e-value: 0.0
score: 472.4
IPR002684Biotin synthase/Biotin biosynthesis bifunctional protein BioABTIGRFAMTIGR00433TIGR00433coord: 17..302
e-value: 4.8E-112
score: 372.0
IPR002684Biotin synthase/Biotin biosynthesis bifunctional protein BioABPANTHERPTHR22976BIOTIN SYNTHASEcoord: 17..321
IPR002684Biotin synthase/Biotin biosynthesis bifunctional protein BioABHAMAPMF_01694BioBcoord: 4..305
score: 38.520813
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 40..198
e-value: 1.1E-13
score: 51.9
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 28..257
score: 32.547581
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 7..306
e-value: 8.0E-130
score: 434.6
NoneNo IPR availableSFLDSFLDG01278biotin_synthase_likecoord: 18..309
e-value: 0.0
score: 472.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 310..329
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 40..240
e-value: 2.63286E-15
score: 71.5957
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 18..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10003802.1HG10003802.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009102 biotin biosynthetic process
cellular_component GO:0005634 nucleus
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0004076 biotin synthase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding