HG10003235 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10003235
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	(-)-germacrene D synthase-like
Location	Chr11: 19098308 .. 19099613 (+)
RNA-Seq Expression	HG10003235
Synteny	HG10003235

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGAATTATGTTTTGCACGAGATAGGGTGGTGGAGTGCTGTATTTGGGCATTGGAAGTATATTATGAACCAAAGTACTCTACCAATAGAAGAATACTTCTCAAAACTATTTCATTCATAACCCTTATTGATGATATATATGATGCATACGCCACATTGGATGAACTTCAACTTTTCACCGAAGCAATTCAAAGGTTTATCTCTCAAAACTCTTTGTTTGATTTAATGTTTAGATGTAAAGCTTGCCTAGCTAGTTTTTCAATATTCATTTTTAGTTTAGTAGTGATTTTGAGCATTAGAAATTTCAAGTTTAAATTGATGTATTGTTGGATATATTGGCCTTGTTCACCAAATTATTTATTGTTCTCCACATATGATCTGATAGGATACATTGTTAAGAAGTTATATTAAATGATTAAATATATTTTATGAGCATTTAGTGATTTTATGTAATCATTACCGATAATATATAGGTTTAAATACTACTTTATTCCTTATACTTTTAGTTTTAGTTCATTTTAGTACTCTTTATACTTTTAAATTGTTCATTTTGGTCCCTATATTTTGAAAATGTTCATTTTTGGTCCCTATACTTTGGTTCATTTTGGTCCTTATACTTTTAAAAAGTTGCTATTTTGGTCCCTTCATTTTCATTTTTAAATGATAAAAATGGTTACTTTTTCGAAAGTTCAAGGACCAAAATGAACCAAAGTTGAAAGTACAAGGACCAAAATGAACCAAAGTTGAAAGTATAGGGACCAAAATGAACAAAAAGTAAAAGTAAAAGGACCAACTAGTATTTAAACATAATATATATGATATATATTTTTAAATTATTCTAAAAAGATTAAGGACAACAAATGTCCGCATATATCTCCATAATGGTAAGGTCGTATTTTTGCAACTCGTGCATATATAAACCCTCATAGTTTTGTTTTGGAACTATCCAAAACGCTTTCTAACAATGGAGAAAATCTGTCTTCATTGGTATATCGTGGATCTCTCCCTTCAATTCCTTAGCACCTAATACAAATGTTTGCGAGAATTTAACATATTAAATAATTATTCTTTATTCTAGCCTTATAATTCTTTGAACTTTTGTTTAAATTTCAGATGGGATATCAATAGCATTGAGAAGCTCTCAAATTACATGAAAGGTCTTTATGAAGCTATACTTGAATTTTATGGAGAGATTGAACAAGATATGTGCATGGATCACAATATTACATTTGCATTTGATTGCGCAAAAGAAGCGGAAAATTACTTAAGAAGATGGTTAAAATACCATGCTTTTTGGCTGTAG

mRNA sequence

ATGAAAGAATTATGTTTTGCACGAGATAGGGTGGTGGAGTGCTGTATTTGGGCATTGGAAGTATATTATGAACCAAAGTACTCTACCAATAGAAGAATACTTCTCAAAACTATTTCATTCATAACCCTTATTGATGATATATATGATGCATACGCCACATTGGATGAACTTCAACTTTTCACCGAAGCAATTCAAAGATGGGATATCAATAGCATTGAGAAGCTCTCAAATTACATGAAAGGTCTTTATGAAGCTATACTTGAATTTTATGGAGAGATTGAACAAGATATGTGCATGGATCACAATATTACATTTGCATTTGATTGCGCAAAAGAAGCGGAAAATTACTTAAGAAGATGGTTAAAATACCATGCTTTTTGGCTGTAG

Coding sequence (CDS)

ATGAAAGAATTATGTTTTGCACGAGATAGGGTGGTGGAGTGCTGTATTTGGGCATTGGAAGTATATTATGAACCAAAGTACTCTACCAATAGAAGAATACTTCTCAAAACTATTTCATTCATAACCCTTATTGATGATATATATGATGCATACGCCACATTGGATGAACTTCAACTTTTCACCGAAGCAATTCAAAGATGGGATATCAATAGCATTGAGAAGCTCTCAAATTACATGAAAGGTCTTTATGAAGCTATACTTGAATTTTATGGAGAGATTGAACAAGATATGTGCATGGATCACAATATTACATTTGCATTTGATTGCGCAAAAGAAGCGGAAAATTACTTAAGAAGATGGTTAAAATACCATGCTTTTTGGCTGTAG

Protein sequence

MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLFTEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNITFAFDCAKEAENYLRRWLKYHAFWL

Homology

[Close]

BLAST of HG10003235 vs. NCBI nr
Match: XP_038904198.1 (sesquiterpene synthase-like [Benincasa hispida])

HSP 1 Score: 199.1 bits (505), Expect = 2.3e-47
Identity = 93/113 (82.30%), Postives = 102/113 (90.27%), Query Frame = 0


Query: 1   MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLF 60
           M++L FARDRVVEC IWAL VYYEPKYSTNRRI +K I FIT+IDD+YDAY  LDELQLF
Sbjct: 47  MEQLSFARDRVVECYIWALGVYYEPKYSTNRRIFVKIIYFITIIDDVYDAYGILDELQLF 106

Query: 61  TEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNITFAFDCAKEA 114
           T+AIQRWDI S++KL NYMKGLYEAILEFY EIEQDMCMD+NITFAFDCAKEA
Sbjct: 107 TQAIQRWDIKSVDKLPNYMKGLYEAILEFYREIEQDMCMDNNITFAFDCAKEA 159

[Close]

BLAST of HG10003235 vs. NCBI nr
Match: XP_038904196.1 (sesquiterpene synthase-like [Benincasa hispida])

HSP 1 Score: 199.1 bits (505), Expect = 2.3e-47
Identity = 93/113 (82.30%), Postives = 102/113 (90.27%), Query Frame = 0


Query: 1   MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLF 60
           M++L FARDRVVEC IWAL VYYEPKYSTNRRI +K I FIT+IDD+YDAY  LDELQLF
Sbjct: 47  MEQLSFARDRVVECYIWALGVYYEPKYSTNRRIFVKIIYFITIIDDVYDAYGILDELQLF 106

Query: 61  TEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNITFAFDCAKEA 114
           T+AIQRWDI S++KL NYMKGLYEAILEFY EIEQDMCMD+NITFAFDCAKEA
Sbjct: 107 TQAIQRWDIKSVDKLPNYMKGLYEAILEFYREIEQDMCMDNNITFAFDCAKEA 159

[Close]

BLAST of HG10003235 vs. NCBI nr
Match: XP_031738277.1 ((-)-germacrene D synthase isoform X2 [Cucumis sativus])

HSP 1 Score: 177.9 bits (450), Expect = 5.4e-41
Identity = 87/129 (67.44%), Postives = 101/129 (78.29%), Query Frame = 0


Query: 1   MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLF 60
           M++L FARDRVVEC +W +E Y +P Y  NRRI LK ++F+T+IDD+YD YATLDELQLF
Sbjct: 274 MEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLF 333

Query: 61  TEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMD-HNITFAFDCAKEAENYLRR 120
           T+AIQRWDI SIEKL NYMKGLYEAILEF+GEIEQDM MD +NI FA DCAKEA     +
Sbjct: 334 TDAIQRWDIKSIEKLPNYMKGLYEAILEFFGEIEQDMSMDNNNIPFALDCAKEALKRQCK 393

Query: 121 WLKYHAFWL 129
                A WL
Sbjct: 394 AYLVEAKWL 402

[Close]

BLAST of HG10003235 vs. NCBI nr
Match: XP_011650452.2 ((-)-germacrene D synthase isoform X1 [Cucumis sativus] >XP_031738276.1 (-)-germacrene D synthase isoform X1 [Cucumis sativus] >KAE8650088.1 hypothetical protein Csa_009890 [Cucumis sativus])

HSP 1 Score: 177.9 bits (450), Expect = 5.4e-41
Identity = 87/129 (67.44%), Postives = 101/129 (78.29%), Query Frame = 0


Query: 1   MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLF 60
           M++L FARDRVVEC +W +E Y +P Y  NRRI LK ++F+T+IDD+YD YATLDELQLF
Sbjct: 168 MEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLF 227

Query: 61  TEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMD-HNITFAFDCAKEAENYLRR 120
           T+AIQRWDI SIEKL NYMKGLYEAILEF+GEIEQDM MD +NI FA DCAKEA     +
Sbjct: 228 TDAIQRWDIKSIEKLPNYMKGLYEAILEFFGEIEQDMSMDNNNIPFALDCAKEALKRQCK 287

Query: 121 WLKYHAFWL 129
                A WL
Sbjct: 288 AYLVEAKWL 296

[Close]

BLAST of HG10003235 vs. NCBI nr
Match: XP_008448975.1 (PREDICTED: (-)-germacrene D synthase-like [Cucumis melo])

HSP 1 Score: 176.8 bits (447), Expect = 1.2e-40
Identity = 84/114 (73.68%), Postives = 99/114 (86.84%), Query Frame = 0


Query: 1   MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLF 60
           M++L FARDRVVEC +WALE +YEPKYS NRRILLK I F+T+IDD+YDAYAT+DELQLF
Sbjct: 275 MEQLHFARDRVVECYLWALEAFYEPKYSANRRILLKMIYFVTIIDDMYDAYATVDELQLF 334

Query: 61  TEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNIT-FAFDCAKEA 114
           TEAIQRWD  SIEKL NYMKGLY+AIL+F+GEIE+DM MD++   FA D AKE+
Sbjct: 335 TEAIQRWDTKSIEKLPNYMKGLYQAILDFFGEIEEDMSMDNSTAPFALDSAKES 388

[Close]

BLAST of HG10003235 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.6e-27
Identity = 63/127 (49.61%), Postives = 90/127 (70.87%), Query Frame = 0


Query: 3   ELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLFTE 62
           +L FARDRVVEC  W L VY+EP++   RRIL K I+  ++IDDIYD Y TL+EL+LFTE
Sbjct: 268 KLPFARDRVVECYFWILGVYFEPQFFFARRILTKVIAMTSIIDDIYDVYGTLEELELFTE 327

Query: 63  AIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNITFAFDCAKEA-ENYLRRWL 122
           A++RWDI++I++L  YM+  Y+A+L  Y EIE++M  +   ++    AKEA +N +R + 
Sbjct: 328 AVERWDISAIDQLPEYMRVCYQALLYVYSEIEEEMAKEGR-SYRLYYAKEAMKNQVRAYY 387

Query: 123 KYHAFWL 129
           +  A WL
Sbjct: 388 E-EAKWL 392

[Close]

BLAST of HG10003235 vs. ExPASy Swiss-Prot
Match: Q84LF2 (5-epi-aristolochene synthase 3 OS=Nicotiana attenuata OX=49451 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 3.5e-27
Identity = 60/117 (51.28%), Postives = 84/117 (71.79%), Query Frame = 0


Query: 4   LCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLFTEA 63
           L +ARDRVVEC  WAL VY+EP+YS  R IL+KTIS I+++DD +DAY T+ EL+ +T+A
Sbjct: 260 LPYARDRVVECYFWALGVYFEPQYSQARVILVKTISMISIVDDTFDAYGTIKELETYTDA 319

Query: 64  IQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMC---MDHNITFAFDCAKE-AENY 117
           IQRWDIN I++L +YMK  Y+AIL+ Y + E+++      H +  A +  KE  +NY
Sbjct: 320 IQRWDINEIDRLPDYMKISYKAILDLYKDYEKELSSAGRSHIVCHAIERMKEVVKNY 376

[Close]

BLAST of HG10003235 vs. ExPASy Swiss-Prot
Match: O65323 (5-epiaristolochene synthase OS=Capsicum annuum OX=4072 GN=EAS PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 3.5e-27
Identity = 58/112 (51.79%), Postives = 81/112 (72.32%), Query Frame = 0


Query: 4   LCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLFTEA 63
           L +ARDRVVEC  WAL VYYEP+YS  R +L+KTI+ I+++DD YDAY T+DEL ++T+ 
Sbjct: 271 LPYARDRVVECYFWALGVYYEPQYSQARVMLVKTIAMISIVDDTYDAYGTVDELAIYTDV 330

Query: 64  IQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMD---HNITFAFDCAKE 113
           IQRWDI  I+ L +YMK  Y+A+L+ Y + E++M  D   H + +A +  KE
Sbjct: 331 IQRWDIKEIDSLPDYMKISYKALLDLYKDYEKEMSRDGRSHVVYYAKERLKE 382

[Close]

BLAST of HG10003235 vs. ExPASy Swiss-Prot
Match: F6M8H4 (Probable sesquiterpene synthase OS=Santalum album OX=35974 GN=SesquiTPS1 PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 4.6e-27
Identity = 61/122 (50.00%), Postives = 82/122 (67.21%), Query Frame = 0


Query: 6   FARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLFTEAIQ 65
           FARDR+VE   W L VY+EP+YS  R+I++K  + I+ IDDIYDAY T+DEL+LFTEAI+
Sbjct: 273 FARDRLVEGYFWVLGVYFEPQYSLARKIIIKVFTMISTIDDIYDAYGTVDELKLFTEAIR 332

Query: 66  RWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNITFAFDCAKEAENYLRRWLKYHA 125
           RWD+ S+++L  YMK  Y++ILE Y EIE++M    ++ F    AKE    L       A
Sbjct: 333 RWDVGSLDQLPEYMKPCYKSILEVYNEIEEEMDNQGSL-FRMHYAKEVMKKLVEGYMDEA 392

Query: 126 FW 128
            W
Sbjct: 393 KW 393

[Close]

BLAST of HG10003235 vs. ExPASy Swiss-Prot
Match: F6M8H7 (Probable sesquiterpene synthase OS=Santalum murrayanum OX=453085 GN=STPS PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 6.0e-27
Identity = 57/107 (53.27%), Postives = 79/107 (73.83%), Query Frame = 0


Query: 6   FARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLFTEAIQ 65
           FARDR+VE   W L VY+EP+YS  R+I++K  + I+ IDDIYDAY TLDEL+LFT+A+Q
Sbjct: 276 FARDRLVEGYFWVLGVYFEPQYSLARKIIIKVFTMISTIDDIYDAYGTLDELKLFTKAMQ 335

Query: 66  RWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNITFAFDCAKE 113
           RWD+ S+++L  YMK  Y++IL+ Y EIE++M    ++ F    AKE
Sbjct: 336 RWDVGSLDQLPEYMKPCYKSILDVYNEIEEEMANQGSL-FRMHYAKE 381

[Close]

BLAST of HG10003235 vs. ExPASy TrEMBL
Match: A0A0A0L782 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G040850 PE=4 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.6e-41
Identity = 87/129 (67.44%), Postives = 101/129 (78.29%), Query Frame = 0


Query: 1   MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLF 60
           M++L FARDRVVEC +W +E Y +P Y  NRRI LK ++F+T+IDD+YD YATLDELQLF
Sbjct: 274 MEQLHFARDRVVECYLWVIEAYNKPNYCANRRIFLKMVNFLTIIDDMYDTYATLDELQLF 333

Query: 61  TEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMD-HNITFAFDCAKEAENYLRR 120
           T+AIQRWDI SIEKL NYMKGLYEAILEF+GEIEQDM MD +NI FA DCAKEA     +
Sbjct: 334 TDAIQRWDIKSIEKLPNYMKGLYEAILEFFGEIEQDMSMDNNNIPFALDCAKEALKRQCK 393

Query: 121 WLKYHAFWL 129
                A WL
Sbjct: 394 AYLVEAKWL 402

[Close]

BLAST of HG10003235 vs. ExPASy TrEMBL
Match: A0A1S3BLW6 ((-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103490985 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 5.8e-41
Identity = 84/114 (73.68%), Postives = 99/114 (86.84%), Query Frame = 0


Query: 1   MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLF 60
           M++L FARDRVVEC +WALE +YEPKYS NRRILLK I F+T+IDD+YDAYAT+DELQLF
Sbjct: 275 MEQLHFARDRVVECYLWALEAFYEPKYSANRRILLKMIYFVTIIDDMYDAYATVDELQLF 334

Query: 61  TEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNIT-FAFDCAKEA 114
           TEAIQRWD  SIEKL NYMKGLY+AIL+F+GEIE+DM MD++   FA D AKE+
Sbjct: 335 TEAIQRWDTKSIEKLPNYMKGLYQAILDFFGEIEEDMSMDNSTAPFALDSAKES 388

[Close]

BLAST of HG10003235 vs. ExPASy TrEMBL
Match: A0A0A0L791 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G042380 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 5.1e-37
Identity = 83/128 (64.84%), Postives = 97/128 (75.78%), Query Frame = 0


Query: 1   MKELC-FARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQL 60
           +KEL  FAR RVVE  +WAL + YEPKYS  RRIL+K I+FI+L+DD+YD YATLDELQL
Sbjct: 171 LKELLPFARVRVVEIYVWALGIQYEPKYSNTRRILVKIITFISLLDDMYDVYATLDELQL 230

Query: 61  FTEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNITFAFDCAKEAENYLRR 120
           FT AIQRWD++ IEKL NYMKGLYE +LE Y EIEQD+  D+NI FAFD AKEA   L +
Sbjct: 231 FTNAIQRWDVSCIEKLPNYMKGLYETVLEVYEEIEQDISKDNNIPFAFDYAKEAMKSLCK 290

Query: 121 WLKYHAFW 128
                A W
Sbjct: 291 AYFMEAKW 298

[Close]

BLAST of HG10003235 vs. ExPASy TrEMBL
Match: A0A6J1CQS0 ((-)-germacrene D synthase-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111013331 PE=4 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 8.7e-37
Identity = 78/127 (61.42%), Postives = 95/127 (74.80%), Query Frame = 0


Query: 1   MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLF 60
           M++  FARDRVVEC  W L +YYEPKYS  RRIL+K I+FI+L+DD+ DAYATLDELQLF
Sbjct: 166 MEQFSFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMXDAYATLDELQLF 225

Query: 61  TEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNITFAFDCAKEAENYLRRW 120
           T+AI+RWDIN I+KL NYMK + EAIL+ Y E+EQD+  D+NI F  DCAKE+     R 
Sbjct: 226 TDAIERWDINGIDKLPNYMKVIXEAILQVYREVEQDIGKDNNILFGLDCAKESMKRQCRA 285

Query: 121 LKYHAFW 128
               A W
Sbjct: 286 YLVEAKW 292

[Close]

BLAST of HG10003235 vs. ExPASy TrEMBL
Match: A0A6J1CQA8 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013331 PE=4 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 8.7e-37
Identity = 78/127 (61.42%), Postives = 95/127 (74.80%), Query Frame = 0


Query: 1   MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLF 60
           M++  FARDRVVEC  W L +YYEPKYS  RRIL+K I+FI+L+DD+ DAYATLDELQLF
Sbjct: 266 MEQFSFARDRVVECYFWTLGIYYEPKYSNARRILVKVIAFISLLDDMXDAYATLDELQLF 325

Query: 61  TEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMCMDHNITFAFDCAKEAENYLRRW 120
           T+AI+RWDIN I+KL NYMK + EAIL+ Y E+EQD+  D+NI F  DCAKE+     R 
Sbjct: 326 TDAIERWDINGIDKLPNYMKVIXEAILQVYREVEQDIGKDNNILFGLDCAKESMKRQCRA 385

Query: 121 LKYHAFW 128
               A W
Sbjct: 386 YLVEAKW 392

[Close]

BLAST of HG10003235 vs. TAIR 10
Match: AT4G16730.1 (terpene synthase 02 )

HSP 1 Score: 99.0 bits (245), Expect = 3.0e-21
Identity = 43/95 (45.26%), Postives = 63/95 (66.32%), Query Frame = 0


Query: 1   MKELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLF 60
           MK+L F RDR+ E   W + ++YEP++   R+IL K    I ++DDIYD Y TL+EL+LF
Sbjct: 245 MKQLDFVRDRITESYFWTIGIFYEPEFKYCRKILTKIFMLIVIMDDIYDIYGTLEELELF 304

Query: 61  TEAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQ 96
           T  +++WD+N +E+L NYM+  +   L  Y EI Q
Sbjct: 305 TNVVEKWDVNHVERLPNYMRMCF---LFLYNEINQ 336

[Close]

BLAST of HG10003235 vs. TAIR 10
Match: AT4G16740.1 (terpene synthase 03 )

HSP 1 Score: 89.7 bits (221), Expect = 1.8e-18
Identity = 40/79 (50.63%), Postives = 56/79 (70.89%), Query Frame = 0


Query: 2   KELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLFT 61
           K L F RDR+ E    ++ V YEP+++ +R++L K    IT IDDIYD Y TL+ELQLFT
Sbjct: 277 KHLDFVRDRITEGYFSSVGVMYEPEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFT 336

Query: 62  EAIQRWDINSIEKLSNYMK 81
             +++WD+N +E+L NYMK
Sbjct: 337 TIVEKWDVNRLEELPNYMK 355

[Close]

BLAST of HG10003235 vs. TAIR 10
Match: AT4G16740.2 (terpene synthase 03 )

HSP 1 Score: 89.7 bits (221), Expect = 1.8e-18
Identity = 40/79 (50.63%), Postives = 56/79 (70.89%), Query Frame = 0


Query: 2   KELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLFT 61
           K L F RDR+ E    ++ V YEP+++ +R++L K    IT IDDIYD Y TL+ELQLFT
Sbjct: 277 KHLDFVRDRITEGYFSSVGVMYEPEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFT 336

Query: 62  EAIQRWDINSIEKLSNYMK 81
             +++WD+N +E+L NYMK
Sbjct: 337 TIVEKWDVNRLEELPNYMK 355

[Close]

BLAST of HG10003235 vs. TAIR 10
Match: AT2G24210.1 (terpene synthase 10 )

HSP 1 Score: 88.2 bits (217), Expect = 5.2e-18
Identity = 43/97 (44.33%), Postives = 59/97 (60.82%), Query Frame = 0


Query: 2   KELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLFT 61
           K+L FARDR+VE   W +    EP+Y   R+ + K  + +T IDDIYD Y TL+ELQLFT
Sbjct: 296 KQLYFARDRIVENYFWTIGQIQEPQYGYVRQTMTKINALLTTIDDIYDIYGTLEELQLFT 355

Query: 62  EAIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDMC 99
            A + WDIN +++L  YM+  +   L  Y E+    C
Sbjct: 356 VAFENWDINRLDELPEYMRLCF---LVIYNEVNSIAC 389

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BLAST of HG10003235 vs. TAIR 10
Match: AT3G14490.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.0e-17
Identity = 40/95 (42.11%), Postives = 60/95 (63.16%), Query Frame = 0


Query: 3   ELCFARDRVVECCIWALEVYYEPKYSTNRRILLKTISFITLIDDIYDAYATLDELQLFTE 62
           +L + RDR+VE    AL +Y+EP+YS  R I+ K    +T+ +D  DAY TL E+    +
Sbjct: 312 KLPYIRDRIVEVYFGALALYFEPRYSLGRIIVTKITMIVTVFNDTCDAYGTLPEVTSLVD 371

Query: 63  AIQRWDINSIEKLSNYMKGLYEAILEFYGEIEQDM 98
           + QRWD+  IEKL +Y+K ++  + E   EIEQ+M
Sbjct: 372 SFQRWDLGDIEKLPSYVKIVFRGVFETLEEIEQEM 406

The following BLAST results are available for this feature:

BLAST of HG10003235 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904198.1	2.3e-47	82.30	sesquiterpene synthase-like [Benincasa hispida]	[more]
XP_038904196.1	2.3e-47	82.30	sesquiterpene synthase-like [Benincasa hispida]	[more]
XP_031738277.1	5.4e-41	67.44	(-)-germacrene D synthase isoform X2 [Cucumis sativus]	[more]
XP_011650452.2	5.4e-41	67.44	(-)-germacrene D synthase isoform X1 [Cucumis sativus] >XP_031738276.1 (-)-germa...	[more]
XP_008448975.1	1.2e-40	73.68	PREDICTED: (-)-germacrene D synthase-like [Cucumis melo]	[more]

BLAST of HG10003235 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6Q3H3	1.6e-27	49.61	(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S...	[more]
Q84LF2	3.5e-27	51.28	5-epi-aristolochene synthase 3 OS=Nicotiana attenuata OX=49451 PE=1 SV=1	[more]
O65323	3.5e-27	51.79	5-epiaristolochene synthase OS=Capsicum annuum OX=4072 GN=EAS PE=1 SV=1	[more]
F6M8H4	4.6e-27	50.00	Probable sesquiterpene synthase OS=Santalum album OX=35974 GN=SesquiTPS1 PE=3 SV...	[more]
F6M8H7	6.0e-27	53.27	Probable sesquiterpene synthase OS=Santalum murrayanum OX=453085 GN=STPS PE=3 SV...	[more]

BLAST of HG10003235 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L782	2.6e-41	67.44	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G040850 PE=4 SV=1	[more]
A0A1S3BLW6	5.8e-41	73.68	(-)-germacrene D synthase-like OS=Cucumis melo OX=3656 GN=LOC103490985 PE=4 SV=1	[more]
A0A0A0L791	5.1e-37	64.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G042380 PE=4 SV=1	[more]
A0A6J1CQS0	8.7e-37	61.42	(-)-germacrene D synthase-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1CQA8	8.7e-37	61.42	(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]

BLAST of HG10003235 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16730.1	3.0e-21	45.26	terpene synthase 02	[more]
AT4G16740.1	1.8e-18	50.63	terpene synthase 03	[more]
AT4G16740.2	1.8e-18	50.63	terpene synthase 03	[more]
AT2G24210.1	5.2e-18	44.33	terpene synthase 10	[more]
AT3G14490.1	2.0e-17	42.11	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005630	Terpene synthase, metal-binding domain	PFAM	PF03936	Terpene_synth_C	coord: 2..121 e-value: 7.1E-37 score: 127.2
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 1..127 e-value: 7.6E-42 score: 145.4
IPR008949	Isoprenoid synthase domain superfamily	SUPERFAMILY	48576	Terpenoid synthases	coord: 3..122
None	No IPR available	PANTHER	PTHR31225:SF42	5-EPIARISTOLOCHENE SYNTHASE	coord: 2..118
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 2..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10003235.1	HG10003235.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0016829	lyase activity