HG10003064 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10003064
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	protein N-lysine methyltransferase METTL21A-like
Location	Chr11: 16923758 .. 16924450 (-)
RNA-Seq Expression	HG10003064
Synteny	HG10003064

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCGTTAGAGAATTCCAAATTGGTGATAGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGTAGAGTGCTCACCGGTTCCTGGGTTTGGAACTCGGCATTCGTCCTAGCCGAGTGGATGGAAACTCAGTGCAAGCTATTCAATTTCAACTTCCAGGGCAAGACCGTAATCGAGCTTGGCGCTGGGGCTGGACTCCCAGGTTTGATGGCAGCTTTACTTGGTGCCAGTCGTGTTGTACTTACTGATGTAGAGCCATTGCTTTCTGGGTTGTTGAAGAATGCAGATGCCAACAGCCTTGGTGATCGAGTTGAAGTAAGGGAACTCGTCTGGGGGTCGGATGAATCGGTGAGTCAAGCAAATGAGTTAGGAGAGTTTGACTTGATTTTGATGTCGGATTTGTTCTATGACCCGCAGGACATGCCCCCTCTAGCATCCATCCTAAAGATCATCTCTGGGAAAGATAGCAAAATCTGGGCAGCTTCCGAGATCCGACCATGGACCATCGAGTGCCTCACAGAGTTCACAAATCAAGGGTTCGAGATCAAAGAGTCACCAGTTCAACATGATGAACCTCGTGTGGAAGACGAGGATAACAATTTAGAATCATTTTCTATTTTCCATCTCAGACAACCAGATGAAGAAAGTCAATCCAATCTCTCGACAAGTTCAAGCTAG

mRNA sequence

ATGGGCGTTAGAGAATTCCAAATTGGTGATAGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGTAGAGTGCTCACCGGTTCCTGGGTTTGGAACTCGGCATTCGTCCTAGCCGAGTGGATGGAAACTCAGTGCAAGCTATTCAATTTCAACTTCCAGGGCAAGACCGTAATCGAGCTTGGCGCTGGGGCTGGACTCCCAGGTTTGATGGCAGCTTTACTTGGTGCCAGTCGTGTTGTACTTACTGATGTAGAGCCATTGCTTTCTGGGTTGTTGAAGAATGCAGATGCCAACAGCCTTGGTGATCGAGTTGAAGTAAGGGAACTCGTCTGGGGGTCGGATGAATCGGTGAGTCAAGCAAATGAGTTAGGAGAGTTTGACTTGATTTTGATGTCGGATTTGTTCTATGACCCGCAGGACATGCCCCCTCTAGCATCCATCCTAAAGATCATCTCTGGGAAAGATAGCAAAATCTGGGCAGCTTCCGAGATCCGACCATGGACCATCGAGTGCCTCACAGAGTTCACAAATCAAGGGTTCGAGATCAAAGAGTCACCAGTTCAACATGATGAACCTCGTGTGGAAGACGAGGATAACAATTTAGAATCATTTTCTATTTTCCATCTCAGACAACCAGATGAAGAAAGTCAATCCAATCTCTCGACAAGTTCAAGCTAG

Coding sequence (CDS)

ATGGGCGTTAGAGAATTCCAAATTGGTGATAGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGTAGAGTGCTCACCGGTTCCTGGGTTTGGAACTCGGCATTCGTCCTAGCCGAGTGGATGGAAACTCAGTGCAAGCTATTCAATTTCAACTTCCAGGGCAAGACCGTAATCGAGCTTGGCGCTGGGGCTGGACTCCCAGGTTTGATGGCAGCTTTACTTGGTGCCAGTCGTGTTGTACTTACTGATGTAGAGCCATTGCTTTCTGGGTTGTTGAAGAATGCAGATGCCAACAGCCTTGGTGATCGAGTTGAAGTAAGGGAACTCGTCTGGGGGTCGGATGAATCGGTGAGTCAAGCAAATGAGTTAGGAGAGTTTGACTTGATTTTGATGTCGGATTTGTTCTATGACCCGCAGGACATGCCCCCTCTAGCATCCATCCTAAAGATCATCTCTGGGAAAGATAGCAAAATCTGGGCAGCTTCCGAGATCCGACCATGGACCATCGAGTGCCTCACAGAGTTCACAAATCAAGGGTTCGAGATCAAAGAGTCACCAGTTCAACATGATGAACCTCGTGTGGAAGACGAGGATAACAATTTAGAATCATTTTCTATTTTCCATCTCAGACAACCAGATGAAGAAAGTCAATCCAATCTCTCGACAAGTTCAAGCTAG

Protein sequence

MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGKTVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDESVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEFTNQGFEIKESPVQHDEPRVEDEDNNLESFSIFHLRQPDEESQSNLSTSSS

Homology

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BLAST of HG10003064 vs. NCBI nr
Match: XP_023553140.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.8 bits (961), Expect = 5.4e-100
Identity = 186/224 (83.04%), Postives = 202/224 (90.18%), Query Frame = 0


Query: 1   MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGK 60
           MGVREFQI DRN+VIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWM TQCKLF+F+F+ K
Sbjct: 24  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 83

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDE 120
           TVIELGAGAGLPGLMAALLGA RVVLTDVEPLL GLL+N DAN L DRVEVRELVWG DE
Sbjct: 84  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 143

Query: 121 SVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEF 180
           SVS+ANE GEFDLILMSDLFYD +DM PLA ILKIISGK SKIWAASEIRPWT+ECLTEF
Sbjct: 144 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKHSKIWAASEIRPWTVECLTEF 203

Query: 181 TNQGFEIKESPVQHDEPRVEDEDNN-LESFSIFHLRQPDEESQS 224
           TN+GF+++ES VQ DEPR ED+D+  L+SFSIFHL+ PDEE  S
Sbjct: 204 TNEGFKVEESQVQLDEPREEDDDHTILDSFSIFHLQPPDEERNS 247

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BLAST of HG10003064 vs. NCBI nr
Match: XP_022923423.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata])

HSP 1 Score: 367.9 bits (943), Expect = 6.6e-98
Identity = 183/224 (81.70%), Postives = 200/224 (89.29%), Query Frame = 0


Query: 1   MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGK 60
           MGVREFQI D N+VIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWM TQCKLF+F+F+ K
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDE 120
           TVIELGAGAGLPGLMAALLGA RVVLTDVEPLL GLL+N DAN L DRVEVRELVWG DE
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 SVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEF 180
           SVS+ANE GEFDLILMSDLFYD +DM PLA ILKIISGK+SKIWAASEIRPWT+ECLTEF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 TNQGFEIKESPVQHDEPRVEDEDNN-LESFSIFHLRQPDEESQS 224
            N+GF+++ES VQ DEP  ED+D+  L+SFSIFHL+ PDEE  S
Sbjct: 181 MNEGFKVEESQVQLDEPSEEDDDHAILDSFSIFHLQPPDEERNS 224

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BLAST of HG10003064 vs. NCBI nr
Match: KAG7015681.1 (EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.8 bits (935), Expect = 5.6e-97
Identity = 181/221 (81.90%), Postives = 199/221 (90.05%), Query Frame = 0


Query: 1   MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGK 60
           MGVREFQI D N+VIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWM T+CKLF+F+F+ K
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATRCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDE 120
           TVIELGAGAGLPGLMAALLGA RVVLTDVEPLL GLL+N DAN L DRVEVRELVWG DE
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 SVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEF 180
           SVS+ANE GEFDLILMSDLFYD +DM PLA ILKIISGK+SKIWAASEIRPWT+ECLTEF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 TNQGFEIKESPVQHDEPRVEDEDNN-LESFSIFHLRQPDEE 221
            N+GF+++ES VQ DEPR E +D+  L+SFSIFHL+ PDEE
Sbjct: 181 MNEGFKVEESQVQLDEPREEVDDHAILDSFSIFHLQPPDEE 221

[Close]

BLAST of HG10003064 vs. NCBI nr
Match: KAG6577643.1 (EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 364.4 bits (934), Expect = 7.3e-97
Identity = 181/221 (81.90%), Postives = 198/221 (89.59%), Query Frame = 0


Query: 1   MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGK 60
           MGVREFQI D N+VIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWM TQCKLF+F+F+ K
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDE 120
           TVIELGAGAGLPGLMAALLGA  VVLTDVEPLL GLL+N DAN L DRVEVRELVWG DE
Sbjct: 61  TVIELGAGAGLPGLMAALLGARHVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 SVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEF 180
           SVS+ANE GEFDLILMSDLFYD +DM PLA ILKIISGK+SKIWAASEIRPWT+ECLTEF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 TNQGFEIKESPVQHDEPRVEDEDNN-LESFSIFHLRQPDEE 221
            N+GF+++ES VQ DEPR E +D+  L+SFSIFHL+ PDEE
Sbjct: 181 MNEGFKVEESQVQLDEPREEVDDHAILDSFSIFHLQPPDEE 221

[Close]

BLAST of HG10003064 vs. NCBI nr
Match: XP_022965153.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita maxima])

HSP 1 Score: 363.6 bits (932), Expect = 1.2e-96
Identity = 180/224 (80.36%), Postives = 199/224 (88.84%), Query Frame = 0


Query: 1   MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGK 60
           MGVREFQI DRN+VIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWM TQCKLF+F+ + K
Sbjct: 31  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDIRDK 90

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDE 120
           TVIELGAG GLPGLMAALLGA RVVLTDVEPLL GLL+N DAN L DRV+VRELVWG DE
Sbjct: 91  TVIELGAGVGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLVDRVQVRELVWGLDE 150

Query: 121 SVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEF 180
           SVS+ANELGEFDLILMSDLFYD +DM PLA +LKIISGK+SKIWAASEIRPWT+ECL EF
Sbjct: 151 SVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTVECLKEF 210

Query: 181 TNQGFEIKESPVQHDEPRVEDEDNN-LESFSIFHLRQPDEESQS 224
            N+GF+++ES VQ DEPR ED+D+  L+ FSIFHL+ PDEE  S
Sbjct: 211 MNEGFKVEESQVQLDEPREEDDDHAILDLFSIFHLQPPDEERNS 254

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BLAST of HG10003064 vs. ExPASy Swiss-Prot
Match: A4FV98 (EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.9e-15
Identity = 62/182 (34.07%), Postives = 91/182 (50.00%), Query Frame = 0


Query: 35  VWNSAFVLAEWMETQCKLFNFNFQGKTVIELGAGAGLPGLMAALLGASRVVLTDVEPLLS 94
           VW++A  L  + E+Q    N +F+GK VIELGAG G+ G++AAL G   V +TD+  +L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLVLE 115

Query: 95  GLLKNADAN-SLGDRVEVRELVWGSDESVSQANELGEFDLILMSDLFYDPQDMPPLASIL 154
            +  N  AN   G R +VR L WG D+ V      G++DL+L +D+ Y     P L   L
Sbjct: 116 QIQGNVQANVPPGGRAQVRALSWGIDQHVFP----GDYDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KIISGKDSKIWAASEIRP--WTIECLTEFTNQGFEIKESPVQHDEPRVEDEDNNLESFSI 214
           + + G    I+ AS++R    T         Q F++        E    DED N+  +  
Sbjct: 176 RHLCGPHGTIYLASKMREEHGTESFFQHLLPQHFQL--------ELAQRDEDENVNIYRA 220

[Close]

BLAST of HG10003064 vs. ExPASy Swiss-Prot
Match: D3YWP0 (EEF1A lysine methyltransferase 3 OS=Mus musculus OX=10090 GN=eef1akmt3 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 7.3e-15
Identity = 56/156 (35.90%), Postives = 83/156 (53.21%), Query Frame = 0


Query: 16  HELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGKTVIELGAGAGLPGLM 75
           HEL  +  +   R+   + VW++A  L ++ E+Q    N +F+GK VIELGAG G+ G++
Sbjct: 38  HEL-SITQNFGSRLGVAARVWDAALSLCDYFESQ----NVDFRGKKVIELGAGTGIVGIL 97

Query: 76  AALLGASRVVLTDVEPLLSGLLKNADAN-SLGDRVEVRELVWGSDESVSQANELGEFDLI 135
           AAL G   V +TD+   L  +  N  AN   G R  V  L WG D+ V      G +DL+
Sbjct: 98  AALQGGD-VTITDLPVALEQIQDNVHANVPPGGRARVCALSWGIDQHVFP----GNYDLV 157

Query: 136 LMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIR 171
           L +D+ Y     P L   L+ + G    I+ AS++R
Sbjct: 158 LGADIVYLEPTFPLLLGTLRHLCGPHGTIYLASKMR 183

[Close]

BLAST of HG10003064 vs. ExPASy Swiss-Prot
Match: Q5BLD8 (Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.6e-14
Identity = 61/167 (36.53%), Postives = 87/167 (52.10%), Query Frame = 0


Query: 35  VWNSAFVLAEWMETQCKLFNFNFQGKTVIELGAGAGLPGLMAALLGASRVVLTDVEPLLS 94
           VW++A VL  ++E    +   + +GK VIELGAG GL G++AALLGA+ V +TD EP L 
Sbjct: 46  VWDAAVVLCMFLE----MGKVDLKGKRVIELGAGTGLVGIVAALLGAN-VTITDREPALE 105

Query: 95  GLLKNADANSLGDR---VEVRELVWGSDESVSQANELGEFDLILMSDLFYDPQDMPPLAS 154
            L  N   N    R   V+V EL WG +  +      G +DLIL +D+ Y  +  P L  
Sbjct: 106 FLTANVHENIPQGRQKAVQVSELTWGENLDLYPQ---GGYDLILGADIVYLEETFPALLQ 165

Query: 155 ILKIISGKDSKIWAASEIRPWTIECLTEFTNQGFEIKESPVQHDEPR 199
            L+ +S  D+ +  +  IR    E       Q F ++E  V +D  R
Sbjct: 166 TLEHLSSGDTVVLLSCRIRYERDERFLTELRQRFSVQE--VHYDSQR 202

[Close]

BLAST of HG10003064 vs. ExPASy Swiss-Prot
Match: Q96AZ1 (EEF1A lysine methyltransferase 3 OS=Homo sapiens OX=9606 GN=EEF1AKMT3 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 3.6e-14
Identity = 63/186 (33.87%), Postives = 91/186 (48.92%), Query Frame = 0


Query: 35  VWNSAFVLAEWMETQCKLFNFNFQGKTVIELGAGAGLPGLMAALLGASRVVLTDVEPLLS 94
           VW++A  L  + E+Q    N +F+GK VIELGAG G+ G++AAL G   V +TD+   L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLALE 115

Query: 95  GLLKNADAN-SLGDRVEVRELVWGSDESVSQANELGEFDLILMSDLFYDPQDMPPLASIL 154
            +  N  AN   G + +VR L WG D  V  AN    +DL+L +D+ Y     P L   L
Sbjct: 116 QIQGNVQANVPAGGQAQVRALSWGIDHHVFPAN----YDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KIISGKDSKIWAASEIRP--WTIECLTEFTNQGFEIKESPVQHDEPRVEDEDNNLESFSI 214
           + +      I+ AS++R    T         Q F++        E    DED N+  +  
Sbjct: 176 QHLCRPHGTIYLASKMRKEHGTESFFQHLLPQHFQL--------ELAQRDEDENVNIYRA 223

Query: 215 FHLRQP 218
            H R+P
Sbjct: 236 RH-REP 223

[Close]

BLAST of HG10003064 vs. ExPASy Swiss-Prot
Match: Q8C436 (Protein-lysine methyltransferase METTL21D OS=Mus musculus OX=10090 GN=Vcpkmt PE=2 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 1.8e-13
Identity = 64/195 (32.82%), Postives = 99/195 (50.77%), Query Frame = 0


Query: 32  GSWVWNSAFVLAEWMETQCKLFNFNFQG------KTVIELGAGAGLPGLMAALLGASRVV 91
           G  VW++A VL++++ET      F+  G      ++V+ELG+G G  GLMAA LGA  V+
Sbjct: 39  GCVVWDAAIVLSKYLETP----GFSGDGAHALSRRSVLELGSGTGAVGLMAATLGAD-VI 98

Query: 92  LTDVEPLLSGLLKNADANS--LGDRVEVRELVWGSDESVSQANELGEFDLILMSDLFYDP 151
           +TD+E L   L  N D N   +   V+ + L WG D       +L   D ILM+D  Y  
Sbjct: 99  VTDLEELQDLLKMNIDMNKHLVTGSVQAKVLKWGED-----IEDLMSPDYILMADCIYYE 158

Query: 152 QDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEFTNQGFEIKESPVQHDEPRVE--D 211
           + + PL   LK +SG ++ I    E R  T+    E   + FE+ +     +E  ++  D
Sbjct: 159 ESLEPLLKTLKDLSGSETCIICCYEQR--TMGKNPEIEKKYFELLQLDFDFEEIPLDKHD 218

Query: 212 EDNNLESFSIFHLRQ 217
           E+   E   I ++R+
Sbjct: 219 EEYRSEDIHIVYIRK 221

[Close]

BLAST of HG10003064 vs. ExPASy TrEMBL
Match: A0A6J1E9L9 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 GN=LOC111431122 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.2e-98
Identity = 183/224 (81.70%), Postives = 200/224 (89.29%), Query Frame = 0


Query: 1   MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGK 60
           MGVREFQI D N+VIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWM TQCKLF+F+F+ K
Sbjct: 1   MGVREFQISDTNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDFRDK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDE 120
           TVIELGAGAGLPGLMAALLGA RVVLTDVEPLL GLL+N DAN L DRVEVRELVWG DE
Sbjct: 61  TVIELGAGAGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLEDRVEVRELVWGLDE 120

Query: 121 SVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEF 180
           SVS+ANE GEFDLILMSDLFYD +DM PLA ILKIISGK+SKIWAASEIRPWT+ECLTEF
Sbjct: 121 SVSRANEFGEFDLILMSDLFYDSEDMAPLAHILKIISGKNSKIWAASEIRPWTVECLTEF 180

Query: 181 TNQGFEIKESPVQHDEPRVEDEDNN-LESFSIFHLRQPDEESQS 224
            N+GF+++ES VQ DEP  ED+D+  L+SFSIFHL+ PDEE  S
Sbjct: 181 MNEGFKVEESQVQLDEPSEEDDDHAILDSFSIFHLQPPDEERNS 224

[Close]

BLAST of HG10003064 vs. ExPASy TrEMBL
Match: A0A6J1HN15 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=LOC111465095 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 6.0e-97
Identity = 180/224 (80.36%), Postives = 199/224 (88.84%), Query Frame = 0


Query: 1   MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGK 60
           MGVREFQI DRN+VIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWM TQCKLF+F+ + K
Sbjct: 31  MGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDIRDK 90

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDE 120
           TVIELGAG GLPGLMAALLGA RVVLTDVEPLL GLL+N DAN L DRV+VRELVWG DE
Sbjct: 91  TVIELGAGVGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLVDRVQVRELVWGLDE 150

Query: 121 SVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEF 180
           SVS+ANELGEFDLILMSDLFYD +DM PLA +LKIISGK+SKIWAASEIRPWT+ECL EF
Sbjct: 151 SVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTVECLKEF 210

Query: 181 TNQGFEIKESPVQHDEPRVEDEDNN-LESFSIFHLRQPDEESQS 224
            N+GF+++ES VQ DEPR ED+D+  L+ FSIFHL+ PDEE  S
Sbjct: 211 MNEGFKVEESQVQLDEPREEDDDHAILDLFSIFHLQPPDEERNS 254

[Close]

BLAST of HG10003064 vs. ExPASy TrEMBL
Match: A0A5A7UCU5 (Protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold344G001920 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 5.3e-93
Identity = 175/219 (79.91%), Postives = 191/219 (87.21%), Query Frame = 0


Query: 1   MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGK 60
           M V+EFQI DRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWM TQCKLF+F+ + K
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDE 120
            VIELGAGAGLPGL AALLGA+RVVLTDV+PLL GLL+N DAN LGDRVEVRELVWGS+E
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 SVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEF 180
             SQANELGEFDLILMSDLFYD ++MP LA +LKIISG DSKIWAASEIRPWTIEC+TE 
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 TNQGFEIKESPVQH-DEPRVEDEDNNLESFSIFHLRQPD 219
            NQGF+I ESPVQH DE   ED    L+S+SIFHLRQ +
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLRQEE 219

[Close]

BLAST of HG10003064 vs. ExPASy TrEMBL
Match: A0A1S3BKF2 (protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo OX=3656 GN=LOC103490817 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 5.3e-93
Identity = 175/219 (79.91%), Postives = 191/219 (87.21%), Query Frame = 0


Query: 1   MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGK 60
           M V+EFQI DRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWM TQCKLF+F+ + K
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDE 120
            VIELGAGAGLPGL AALLGA+RVVLTDV+PLL GLL+N DAN LGDRVEVRELVWGS+E
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 SVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEF 180
             SQANELGEFDLILMSDLFYD ++MP LA +LKIISG DSKIWAASEIRPWTIEC+TE 
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 TNQGFEIKESPVQH-DEPRVEDEDNNLESFSIFHLRQPD 219
            NQGF+I ESPVQH DE   ED    L+S+SIFHLRQ +
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLRQEE 219

[Close]

BLAST of HG10003064 vs. ExPASy TrEMBL
Match: A0A0A0L6N4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016950 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.0e-92
Identity = 170/218 (77.98%), Postives = 190/218 (87.16%), Query Frame = 0


Query: 1   MGVREFQIGDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFNFQGK 60
           M V+EFQI DRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WM TQC LF+F+F+ K
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60

Query: 61  TVIELGAGAGLPGLMAALLGASRVVLTDVEPLLSGLLKNADANSLGDRVEVRELVWGSDE 120
            VIELGAG GLPGL AALLGA+RV+LTDVEPLL GLL+N DAN +GDRVEVRELVWGS++
Sbjct: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120

Query: 121 SVSQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTIECLTEF 180
             SQANELGEFDLILMSDLFY+ ++MP LA +LKIISG D+KIWAASEIRPWTIEC+TE 
Sbjct: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180

Query: 181 TNQGFEIKESPVQHDEPRVEDEDNNLESFSIFHLRQPD 219
            NQGF+I ESPVQHDE   ED    L+SFSIFHLRQ +
Sbjct: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEE 218

[Close]

BLAST of HG10003064 vs. TAIR 10
Match: AT1G08125.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.7e-14
Identity = 57/205 (27.80%), Postives = 97/205 (47.32%), Query Frame = 0


Query: 16  HELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFN---FQGKTVIELGAGAGLP 75
           HELD   D  +  +  G+ VW+++ V A+++    +   F+    +GK  IELGAG G+ 
Sbjct: 21  HELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVA 80

Query: 76  GLMAALLGASRVVLTDVEPLLSGLLKNADAN----------SLGDRVEVRELVWGSDESV 135
           G   A+LG   VV TD + +L  L +N + N          S    + V EL WG+++ +
Sbjct: 81  GFALAMLGCD-VVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHI 140

Query: 136 SQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTI-ECLTEFT 195
           +       FD ++ +D+ Y  Q + PL   +  +SG  + +    EIR   + E + +  
Sbjct: 141 TAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMW 200

Query: 196 NQGFEIKESPVQHDEPRVEDEDNNL 207
              FE+K  P    +   +D   +L
Sbjct: 201 KDNFEVKTIPRSKMDGEYQDPSIHL 220

[Close]

BLAST of HG10003064 vs. TAIR 10
Match: AT1G08125.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 3.7e-14
Identity = 57/205 (27.80%), Postives = 97/205 (47.32%), Query Frame = 0


Query: 16  HELDDLYDSTTGRVLTGSWVWNSAFVLAEWMETQCKLFNFN---FQGKTVIELGAGAGLP 75
           HELD   D  +  +  G+ VW+++ V A+++    +   F+    +GK  IELGAG G+ 
Sbjct: 28  HELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVA 87

Query: 76  GLMAALLGASRVVLTDVEPLLSGLLKNADAN----------SLGDRVEVRELVWGSDESV 135
           G   A+LG   VV TD + +L  L +N + N          S    + V EL WG+++ +
Sbjct: 88  GFALAMLGCD-VVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHI 147

Query: 136 SQANELGEFDLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIRPWTI-ECLTEFT 195
           +       FD ++ +D+ Y  Q + PL   +  +SG  + +    EIR   + E + +  
Sbjct: 148 TAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMW 207

Query: 196 NQGFEIKESPVQHDEPRVEDEDNNL 207
              FE+K  P    +   +D   +L
Sbjct: 208 KDNFEVKTIPRSKMDGEYQDPSIHL 227

[Close]

BLAST of HG10003064 vs. TAIR 10
Match: AT1G73320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 7.0e-13
Identity = 49/146 (33.56%), Postives = 75/146 (51.37%), Query Frame = 0


Query: 30  LTGSWVWNSAFVLAEWMETQCKLFNFNFQGKTVIELGAGAGLPGLMAALLGASRVVLTDV 89
           +TGS +W+S  VL +++E        + +GK ++ELG+G GL G +AALLG +  VLTD+
Sbjct: 117 VTGSVMWDSGVVLGKFLEHSVDSKVLSLEGKKIVELGSGCGLVGCIAALLGGN-AVLTDL 176

Query: 90  EPLLSGLLKNAD-----ANSLGDRVEVRELVWGSDESVSQANELGEFDLILMSDLFYDPQ 149
              L  L KN        N+ G  + V+ELVWG D            D +L SD+ Y  +
Sbjct: 177 PDRLRLLKKNIQTNLHRGNTRGSAI-VQELVWGDDPDPDLIEPFP--DYVLGSDVIYSEE 236

Query: 150 DMPPLASILKIISGKDSKIWAASEIR 171
            +  L   L  +    + I+ + E+R
Sbjct: 237 AVHHLVKTLLQLCSDQTTIFLSGELR 258

[Close]

BLAST of HG10003064 vs. TAIR 10
Match: AT5G44170.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 2.0e-12
Identity = 46/184 (25.00%), Postives = 90/184 (48.91%), Query Frame = 0


Query: 27  GRVLTGSWVWNSAFVLAEWMETQCKLFN-------------FNFQGKTVIELGAGAGLPG 86
           G +  G+ VW  + +L+++ E    L +             F+F+ +  IELG G G+ G
Sbjct: 25  GSMHVGTSVWPCSLILSKFAERWSTLDSSSSTTSPNPYAELFDFRRRRGIELGTGCGVAG 84

Query: 87  LMAALLGASRVVLTDVEPLLSGLLKNADAN--SLGDRVEVRELVWGSDESVSQANELGEF 146
           +   LLG + +VLTD+ P++  L  N   N  +LG  ++   + W + + +S       F
Sbjct: 85  MAFYLLGLTEIVLTDIAPVMPALKHNLKRNKTALGKSLKTSIVYWNNRDQISALKP--PF 144

Query: 147 DLILMSDLFYDPQDMPPLASILKIISGKDSKIWAASEIR-PWTIECLTEFTNQGFEIKES 195
           DL++ +D+ Y  + +  L + ++++   D  +    +IR P   +   E  +  F+I++ 
Sbjct: 145 DLVIAADVVYIEESVGQLVTAMELLVADDGAVLLGYQIRSPEADKLFWELCDIVFKIEKV 204

[Close]

BLAST of HG10003064 vs. TAIR 10
Match: AT1G73320.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.5e-10
Identity = 39/95 (41.05%), Postives = 56/95 (58.95%), Query Frame = 0


Query: 30  LTGSWVWNSAFVLAEWMETQCKLFNFNFQGKTVIELGAGAGLPGLMAALLGASRVVLTDV 89
           +TGS +W+S  VL +++E        + +GK ++ELG+G GL G +AALLG +  VLTD+
Sbjct: 117 VTGSVMWDSGVVLGKFLEHSVDSKVLSLEGKKIVELGSGCGLVGCIAALLGGN-AVLTDL 176

Query: 90  EPLLSGLLKNAD-----ANSLGDRVEVRELVWGSD 120
              L  L KN        N+ G  + V+ELVWG D
Sbjct: 177 PDRLRLLKKNIQTNLHRGNTRGSAI-VQELVWGDD 209

The following BLAST results are available for this feature:

BLAST of HG10003064 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023553140.1	5.4e-100	83.04	protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo]	[more]
XP_022923423.1	6.6e-98	81.70	protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata]	[more]
KAG7015681.1	5.6e-97	81.90	EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyros...	[more]
KAG6577643.1	7.3e-97	81.90	EEF1A lysine methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022965153.1	1.2e-96	80.36	protein N-lysine methyltransferase METTL21A-like [Cucurbita maxima]	[more]

BLAST of HG10003064 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A4FV98	1.9e-15	34.07	EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1	[more]
D3YWP0	7.3e-15	35.90	EEF1A lysine methyltransferase 3 OS=Mus musculus OX=10090 GN=eef1akmt3 PE=3 SV=1	[more]
Q5BLD8	1.6e-14	36.53	Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a P...	[more]
Q96AZ1	3.6e-14	33.87	EEF1A lysine methyltransferase 3 OS=Homo sapiens OX=9606 GN=EEF1AKMT3 PE=1 SV=1	[more]
Q8C436	1.8e-13	32.82	Protein-lysine methyltransferase METTL21D OS=Mus musculus OX=10090 GN=Vcpkmt PE=...	[more]

BLAST of HG10003064 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E9L9	3.2e-98	81.70	protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1HN15	6.0e-97	80.36	protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A5A7UCU5	5.3e-93	79.91	Protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BKF2	5.3e-93	79.91	protein N-lysine methyltransferase METTL21A-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A0A0L6N4	2.0e-92	77.98	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G016950 PE=4 SV=1	[more]

BLAST of HG10003064 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08125.1	3.7e-14	27.80	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G08125.2	3.7e-14	27.80	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G73320.1	7.0e-13	33.56	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G44170.1	2.0e-12	25.00	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G73320.2	2.5e-10	41.05	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 18..215 e-value: 5.4E-44 score: 152.4
None	No IPR available	PANTHER	PTHR14614:SF123	OSJNBA0070O11.4 PROTEIN	coord: 1..218
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 61..169 e-value: 6.93134E-6 score: 41.6467
IPR019410	Lysine methyltransferase	PFAM	PF10294	Methyltransf_16	coord: 31..170 e-value: 6.2E-27 score: 94.4
IPR019410	Lysine methyltransferase	PANTHER	PTHR14614	HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN	coord: 1..218
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 43..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10003064.1	HG10003064.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity