HG10002302 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10002302
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionDNA repair protein RadA
LocationChr11: 5377734 .. 5378123 (-)
RNA-Seq ExpressionHG10002302
SyntenyHG10002302
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACATTGCATTCATTGGTGAAATTGGCCTCGGCGGTGAGCTTCGCATGGTAAGCACTCTATTTCAACGTTATTACACAAGTTTTGGCATAACTTACACGAGATCATCTTGTATTTAGTATACCATAAGGGCCTTTTTTCATATTTTAAAAAAAAATGAAATCTCAAAACTTCATTAACGTTCAGGTGGGTAGAATGGAGAAGAGGATCAATACTGTATCCAAATTGGGTTTCAAGAGATGTGTAGTACCCAAATCAGCTGAAAATTGTTTAGGGGTGGTAGAATTGGGCGAGATGAAGCTCATAGGCTGCAGAAATTTGAAAGATGTTATCAACAAAGTGTTTATGGCAAGAGATGAATTTGGAAGTCTACACTCTTTTGTTGGGTAA

mRNA sequence

ATGACATTGCATTCATTGGTGAAATTGGCCTCGGCGGTGGGTAGAATGGAGAAGAGGATCAATACTGTATCCAAATTGGGTTTCAAGAGATGTGTAGTACCCAAATCAGCTGAAAATTGTTTAGGGGTGGTAGAATTGGGCGAGATGAAGCTCATAGGCTGCAGAAATTTGAAAGATGTTATCAACAAAGTGTTTATGGCAAGAGATGAATTTGGAAGTCTACACTCTTTTGTTGGGTAA

Coding sequence (CDS)

ATGACATTGCATTCATTGGTGAAATTGGCCTCGGCGGTGGGTAGAATGGAGAAGAGGATCAATACTGTATCCAAATTGGGTTTCAAGAGATGTGTAGTACCCAAATCAGCTGAAAATTGTTTAGGGGTGGTAGAATTGGGCGAGATGAAGCTCATAGGCTGCAGAAATTTGAAAGATGTTATCAACAAAGTGTTTATGGCAAGAGATGAATTTGGAAGTCTACACTCTTTTGTTGGGTAA

Protein sequence

MTLHSLVKLASAVGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDEFGSLHSFVG
Homology
BLAST of HG10002302 vs. NCBI nr
Match: XP_022153628.1 (uncharacterized protein LOC111021088 [Momordica charantia])

HSP 1 Score: 116.3 bits (290), Expect = 1.2e-22
Identity = 57/66 (86.36%), Postives = 59/66 (89.39%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDEFG 72
           V RMEKRINTV KLGFKRCVVPKSAENCLGVVELG+MKLIGCRNLKDV+N VFMARDE  
Sbjct: 574 VSRMEKRINTVVKLGFKRCVVPKSAENCLGVVELGDMKLIGCRNLKDVMNNVFMARDEAM 633

Query: 73  SLHSFV 79
             HSFV
Sbjct: 634 HRHSFV 639

BLAST of HG10002302 vs. NCBI nr
Match: XP_022980476.1 (uncharacterized protein LOC111479856 [Cucurbita maxima])

HSP 1 Score: 114.8 bits (286), Expect = 3.5e-22
Identity = 57/67 (85.07%), Postives = 59/67 (88.06%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDEFG 72
           V RMEKRINTVSKLGFKRCVVPKSAE CLGVVELGE +LIGCRNLKDVIN VFMARD   
Sbjct: 591 VSRMEKRINTVSKLGFKRCVVPKSAEKCLGVVELGETRLIGCRNLKDVINDVFMARDGAR 650

Query: 73  SLHSFVG 80
           S+ SFVG
Sbjct: 651 SVQSFVG 657

BLAST of HG10002302 vs. NCBI nr
Match: XP_023527681.1 (uncharacterized protein LOC111790828 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 111.3 bits (277), Expect = 3.8e-21
Identity = 55/67 (82.09%), Postives = 58/67 (86.57%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDEFG 72
           V RMEKRINTV+KLGFKRCVVPKSAE CLGVVELGE +LIGC NLKDVIN VFMARD   
Sbjct: 582 VSRMEKRINTVAKLGFKRCVVPKSAEKCLGVVELGETRLIGCSNLKDVINDVFMARDGAR 641

Query: 73  SLHSFVG 80
           S+ SFVG
Sbjct: 642 SVQSFVG 648

BLAST of HG10002302 vs. NCBI nr
Match: KAG7018307.1 (radA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 109.0 bits (271), Expect = 1.9e-20
Identity = 56/68 (82.35%), Postives = 59/68 (86.76%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDEFG 72
           V RMEKRINTV+KLGFKRCVVPKSAE CLGVVE GEM+LIGCRNLKDVIN VFMARD   
Sbjct: 572 VSRMEKRINTVAKLGFKRCVVPKSAEKCLGVVESGEMRLIGCRNLKDVINDVFMARDGAR 631

Query: 73  SLH-SFVG 80
           S+  SFVG
Sbjct: 632 SVRSSFVG 639

BLAST of HG10002302 vs. NCBI nr
Match: XP_022956185.1 (uncharacterized protein LOC111457955 [Cucurbita moschata])

HSP 1 Score: 109.0 bits (271), Expect = 1.9e-20
Identity = 56/68 (82.35%), Postives = 59/68 (86.76%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDEFG 72
           V RMEKRINTV+KLGFKRCVVPKSAE CLGVVE GEM+LIGCRNLKDVIN VFMARD   
Sbjct: 590 VSRMEKRINTVAKLGFKRCVVPKSAEKCLGVVESGEMRLIGCRNLKDVINDVFMARDGAR 649

Query: 73  SLH-SFVG 80
           S+  SFVG
Sbjct: 650 SVQSSFVG 657

BLAST of HG10002302 vs. ExPASy TrEMBL
Match: A0A6J1DHZ5 (uncharacterized protein LOC111021088 OS=Momordica charantia OX=3673 GN=LOC111021088 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 5.7e-23
Identity = 57/66 (86.36%), Postives = 59/66 (89.39%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDEFG 72
           V RMEKRINTV KLGFKRCVVPKSAENCLGVVELG+MKLIGCRNLKDV+N VFMARDE  
Sbjct: 574 VSRMEKRINTVVKLGFKRCVVPKSAENCLGVVELGDMKLIGCRNLKDVMNNVFMARDEAM 633

Query: 73  SLHSFV 79
             HSFV
Sbjct: 634 HRHSFV 639

BLAST of HG10002302 vs. ExPASy TrEMBL
Match: A0A6J1ITP4 (uncharacterized protein LOC111479856 OS=Cucurbita maxima OX=3661 GN=LOC111479856 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.7e-22
Identity = 57/67 (85.07%), Postives = 59/67 (88.06%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDEFG 72
           V RMEKRINTVSKLGFKRCVVPKSAE CLGVVELGE +LIGCRNLKDVIN VFMARD   
Sbjct: 591 VSRMEKRINTVSKLGFKRCVVPKSAEKCLGVVELGETRLIGCRNLKDVINDVFMARDGAR 650

Query: 73  SLHSFVG 80
           S+ SFVG
Sbjct: 651 SVQSFVG 657

BLAST of HG10002302 vs. ExPASy TrEMBL
Match: A0A6J1GX44 (uncharacterized protein LOC111457955 OS=Cucurbita moschata OX=3662 GN=LOC111457955 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 9.2e-21
Identity = 56/68 (82.35%), Postives = 59/68 (86.76%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDEFG 72
           V RMEKRINTV+KLGFKRCVVPKSAE CLGVVE GEM+LIGCRNLKDVIN VFMARD   
Sbjct: 590 VSRMEKRINTVAKLGFKRCVVPKSAEKCLGVVESGEMRLIGCRNLKDVINDVFMARDGAR 649

Query: 73  SLH-SFVG 80
           S+  SFVG
Sbjct: 650 SVQSSFVG 657

BLAST of HG10002302 vs. ExPASy TrEMBL
Match: A0A5D3C9C8 (DNA repair protein RadA isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold323G00200 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.6e-20
Identity = 52/58 (89.66%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDE 71
           VGRMEKRINTV+KLGFKRCVVPKSAENCLGVV LG+MKLIGC NLKDVIN VFM RDE
Sbjct: 570 VGRMEKRINTVAKLGFKRCVVPKSAENCLGVVGLGKMKLIGCTNLKDVINNVFMVRDE 627

BLAST of HG10002302 vs. ExPASy TrEMBL
Match: A0A1S3BHQ7 (DNA repair protein RadA isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489983 PE=4 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.6e-20
Identity = 52/58 (89.66%), Postives = 54/58 (93.10%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVFMARDE 71
           VGRMEKRINTV+KLGFKRCVVPKSAENCLGVV LG+MKLIGC NLKDVIN VFM RDE
Sbjct: 332 VGRMEKRINTVAKLGFKRCVVPKSAENCLGVVGLGKMKLIGCTNLKDVINNVFMVRDE 389

BLAST of HG10002302 vs. TAIR 10
Match: AT5G50340.1 (ATP-dependent peptidases;nucleotide binding;serine-type endopeptidases;DNA helicases;ATP binding;damaged DNA binding;nucleoside-triphosphatases )

HSP 1 Score: 71.2 bits (173), Expect = 4.1e-13
Identity = 32/53 (60.38%), Postives = 43/53 (81.13%), Query Frame = 0

Query: 13  VGRMEKRINTVSKLGFKRCVVPKSAENCLGVVELGEMKLIGCRNLKDVINKVF 66
           V RMEKR++TV+KLGF +CVVPKS E  L  + L E+++IGC+NLK++IN VF
Sbjct: 533 VPRMEKRVSTVAKLGFNKCVVPKSVEESLKALSLKEIEIIGCKNLKELINAVF 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153628.11.2e-2286.36uncharacterized protein LOC111021088 [Momordica charantia][more]
XP_022980476.13.5e-2285.07uncharacterized protein LOC111479856 [Cucurbita maxima][more]
XP_023527681.13.8e-2182.09uncharacterized protein LOC111790828 [Cucurbita pepo subsp. pepo][more]
KAG7018307.11.9e-2082.35radA, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022956185.11.9e-2082.35uncharacterized protein LOC111457955 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DHZ55.7e-2386.36uncharacterized protein LOC111021088 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A6J1ITP41.7e-2285.07uncharacterized protein LOC111479856 OS=Cucurbita maxima OX=3661 GN=LOC111479856... [more]
A0A6J1GX449.2e-2182.35uncharacterized protein LOC111457955 OS=Cucurbita moschata OX=3662 GN=LOC1114579... [more]
A0A5D3C9C81.6e-2089.66DNA repair protein RadA isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BHQ71.6e-2089.66DNA repair protein RadA isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489983 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT5G50340.14.1e-1360.38ATP-dependent peptidases;nucleotide binding;serine-type endopeptidases;DNA helic... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR32472:SF19DNA REPAIR-LIKE PROTEINRADAcoord: 12..66
NoneNo IPR availablePANTHERPTHR32472DNA REPAIR PROTEIN RADAcoord: 12..66

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10002302.1HG10002302.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000725 recombinational repair
cellular_component GO:0009536 plastid
molecular_function GO:0005524 ATP binding
molecular_function GO:0008094 ATP-dependent activity, acting on DNA
molecular_function GO:0003684 damaged DNA binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0046872 metal ion binding