HG10001336 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10001336
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Lysine methyltransferase
Location	Chr09: 16191067 .. 16194713 (+)
RNA-Seq Expression	HG10001336
Synteny	HG10001336

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTCCCAAGTAAGAAAAGCGGAAATACTCACAAGAAGACGAAAGACGCGTTTGGAGGAACCGGGATTGAAGGCGTTGAAGTTGCAGAATTTGCAAAGAGAAGAAGATGGTTTATCAGGAAGTGTTCTCTCACAGATAGAACAAACCCTGTAACAGAAACTAGTGTGATCCATAGCTATCACTGCGCACATGGTTGTTATTGGCTTAGCATTTTCCATTACTCTTCTGAAGGAGACTGCTTCCGTTCCTGAAAACTGAAAACACAGCGGCGCTCCGCCGCTATACACACAAACACTGTTTGGAGGGAAAAGAAGACCGAAATTTCGATATCGTTTTTTATTAGGGGTGTTTTTGTTAATTAAACATAGTACAAGGACACGGACGAAAATCTACCATCTTTCAAATCATCTGGGCCGGCCCAGTCTCCGGATGGGCTTGATGTCAAAAAACGGCCCACTAATTCTACAGTGATTTCATCCGCCGGGAACGGAGGCGATGAAAAACCCAACGAATACAGGAGTCCTGAAATGCACGGAACTGGAGTCTCACTCTTCAAACGAAGAATTAAGAAGCTCCATCAATTTGGTACGTTCATTCTCTTTTTGTGTTTGTCAATCAATTTTATATGCATTAATTTTTCATATTTTTCCTTTTTATTCCATATTAGAGTTGAGTGTTTGATCTTGTCTCTTCTCTTTGGGAAAGAAGGAATTAGTTTAAAGATAATTGTTGGTGCTGTTAATCTGGTTTCTAACGGAATGGAAAAAGGATTTAGACTATGTATGCATATGCAATAACTCATTAGAAGTAGTTCATCATTTTCGTTCAGGTGTTACCTACTCTTTATTTGTCTGATGAGCTAAGCTCCCTAGGTCTCTCTCAACTTCCCCAAATCAAAAGAAAATCTGAATTCTGCATTGCAGCAAATACGTTCTGATCAAAGGTTCAATTACCCTTTTGACTGTGAGGAAGTTGGAATTTGAGGTTCTTGCTATGGAGGCCGACCGGTAATGTAGTCTTAGATTTTTTTTTCTTACCACCTATGCTTGCTGATTCTTTAATATATTATTGAATTTGGTATTATCAGGTTAAATTCTCCCAGTACATTTACCATGACCATGGAAGTATTGGGTCATGAAATGCGATTCTATCAGGTATTGCTTGGAACAGTATTTGAAATTTTCATCGGTCAACATTCTTTCTGATTTGAAGATATAGTGCCAGTCTATCACATCCTTGCCATTTGAAGAACACATCTATGATAGCTCATTGTTATTAGCTAAACTTGCAAAGGGCTATTGTGCTAAAAGTACTCATCCACATTCAAATCCTCTATCCCTTGAGATTTGAGAGAGACATTTCTGTAGTTCATTGCTAACATTTCTGTAGTCCATTGCTAATTTCTATTCAACAGTCCTATCTGTAATAACCTATCTGACCATGGAAGTATGTTACATTGCCATAGGATCCCAATTCGAAACATTTAGGTACGACAGTGTGGGATGCATCACTGGTATTTGTCAAGTTTCTGGTAATCACTGCTCTACCTTTACATTCTCATTGATTCTTATTCGTCATTGGAGTTTCCTTTTTCCTTACAGTATTTGGATTAATATGTATCATCTTACTTACAAACGGAAAATTTAGTTGTTTGTGCGCAAGTTTTCAATTTTGTCAATCTCTGTTAGCAGCATATGACACGCCTAGTCAAATTGAACATATTTTGAATTTGATCATGCTTTCAATTTGTGAAAATGAAAGCATTGCATGGCATTTACTTTGATTATTGGTGGTGTTGGTCTAAAACCTGAGTTTGAGTTATCTTTAATCAAGATGCAAAAACTGAGAGCTGAAAGCTTTGCATCTATTAGATTTTTATAAAATCCCCGATTAATCTGTAAGCATTGAATGTGGAATGTCATTCAATATAACTAAGTTTATCAAAGATAGTTTACTGTAACCCTTCATGTCCATCCCTTATTGGATTAGATGTATTTTTTCATAGGTAGGATGATTTGTACCTTAATTAAATCTGGAAGATTATCATTCTTTTAAAACTTTTGATCTCTTTTTTCACTGTTTCAGGAGAAAAATTGCCGAAAAGGAAAATTTAGCCCTTCTAAACTTAAAGGAAAGCGTGTCATTGAGCTTGGAGCTGGTTGTGGGTTGGCTGGGTTTGGTGAGTTATTTATTTATTTATTATTATTATTTTAATGTAAAGTTTAATCTGAAACCAGTCCTTTTCGTTGCTAATACTTGTGCTTTAAGGGCTCTACCTTCCTTTTTCTCATGTAATTAACAATCTTGATTTGAACTGCTCTTTGTAGGCATGGCATTGCTTGGATGTGATGTAGTTGCTACAGACCAAAGGGAGGTATTGCCTATTCTAACGAGAAATATAGAACGTAATACACCAAGCTTAGCACAGATGAACCCTTCAGGTAGCCTTTCAAAATTATAAATTACAAACTTCCTTATAACAATCATTAGCATTTTAATTCTGGCAATTCAAGTAGAAAAGTTCAGTTTGTTTGAAGTTTGTTTTAGGATTTGAATTCCCCCACCGCCATGTGAAACTTTGTCAAGAGAAAATTATGGTTTTGATTTGGTTATACGGGAGTGATAGTGTTTGTAATTGATTACAGTATGAGAGAACCTTTGATTTTCTTTAGTGCTTCTAATTAGGGAAAGTTGTTCGTTGTAGAATCATTTGGTTCAATTAGGGCAGCAGAATTAGACTGGGGAAATGAAGATCATATCAAGGCAGTAGGTCCACCATGTGACTATATTATCGGCACTGACGTGGTAAGTTTATACGATTGTTTCTTGTTGAAGCACACAATGATACATTATTCAATAACAGATTTTTCTTATGTGATTTACAAAGCGAATAAGTGGATTTTTGTTATTCAACGCGAAGGTTTATGCAGAACATCTATTGGAACCACTTTTAAAGACAATACATGCATTATCAGGTCCCAAAACTACAATTATGGTATGAAATATAACTTTTATCCACATTTTCATATTGGAGATTCTTGTTGGCTTATTTATTCATACTTGTTATGTGAAGCTGGGGTATGAGATTCGTTCGACAAATGTTCACGAGCAAATGCTTGAGATGTGGAAAAAGAACTTTGAGGTGAAAACTGTATCACAATCAAAGGTACACTATACTTCCAACGTTTTTTGAGTCTCTGAAATGGCCATAAATTAAGATGTTAATCCTTGTTTAGTTTTGGGAGAACGTTGGCACAACAATGGTCAGGTTTGCAACATTGACAATAAAGAGGGAACCAGCAAGTCCTCAGCCAAGTACTTAAAACTGTGTTACATTTTTTTATGGTGCTATTTGTTGCAGATGGACTCACAGTATCAGCATCCCAGCATTCAGCTCTACATTATGGGGCTAAAGCCTGCACCTGGGAAAGCAGAGAACACTCGAGACAATGTTGAACAGGAAATTGACACGGTTTGTTCGAGGCAGGAGAATGATGAAAAGGAAGAAAGTTATGACGAAACGGGAATTGAGAAGGCAGAGGAGCCAGTTACAGAACATGAGGATAAGAAATTGGGTGACTGGGAAGCTAGAAGAATGGGCTCTATGGCCGCTCGGCTTCTGCAAGATGTAAAAATTGCTTGA

mRNA sequence

ATGAAATTCCCAAGTAAGAAAAGCGGAAATACTCACAAGAAGACGAAAGACGCGTTTGGAGGAACCGGGATTGAAGGCGTTGAAGTTGCAGAATTTGCAAAGAGAAGAAGATGGTTTATCAGGAAGTGTTCTCTCACAGATAGAACAAACCCTTACAAGGACACGGACGAAAATCTACCATCTTTCAAATCATCTGGGCCGGCCCAGTCTCCGGATGGGCTTGATGTCAAAAAACGGCCCACTAATTCTACAGTGATTTCATCCGCCGGGAACGGAGGCGATGAAAAACCCAACGAATACAGGAGTCCTGAAATGCACGGAACTGGAGTCTCACTCTTCAAACGAAGAATTAAGAAGCTCCATCAATTTGGTTCAATTACCCTTTTGACTGTGAGGAAGTTGGAATTTGAGGTTCTTGCTATGGAGGCCGACCGGTTAAATTCTCCCAGTACATTTACCATGACCATGGAAGTATTGGGTCATGAAATGCGATTCTATCAGGATCCCAATTCGAAACATTTAGGTACGACAGTGTGGGATGCATCACTGGTATTTGTCAAGTTTCTGGAGAAAAATTGCCGAAAAGGAAAATTTAGCCCTTCTAAACTTAAAGGAAAGCGTGTCATTGAGCTTGGAGCTGGTTGTGGGTTGGCTGGGTTTGGCATGGCATTGCTTGGATGTGATGTAGTTGCTACAGACCAAAGGGAGGTATTGCCTATTCTAACGAGAAATATAGAACGTAATACACCAAGCTTAGCACAGATGAACCCTTCAGAATCATTTGGTTCAATTAGGGCAGCAGAATTAGACTGGGGAAATGAAGATCATATCAAGGCAGTAGGTCCACCATGTGACTATATTATCGGCACTGACGTGCGAATAAGTGGATTTTTGTTATTCAACGCGAAGGTTTATGCAGAACATCTATTGGAACCACTTTTAAAGACAATACATGCATTATCAGGTCCCAAAACTACAATTATGCTGGGGTATGAGATTCGTTCGACAAATGTTCACGAGCAAATGCTTGAGATGTGGAAAAAGAACTTTGAGGTGAAAACTGTATCACAATCAAAGATGGACTCACAGTATCAGCATCCCAGCATTCAGCTCTACATTATGGGGCTAAAGCCTGCACCTGGGAAAGCAGAGAACACTCGAGACAATGTTGAACAGGAAATTGACACGGTTTGTTCGAGGCAGGAGAATGATGAAAAGGAAGAAAGTTATGACGAAACGGGAATTGAGAAGGCAGAGGAGCCAGTTACAGAACATGAGGATAAGAAATTGGGTGACTGGGAAGCTAGAAGAATGGGCTCTATGGCCGCTCGGCTTCTGCAAGATGTAAAAATTGCTTGA

Coding sequence (CDS)

ATGAAATTCCCAAGTAAGAAAAGCGGAAATACTCACAAGAAGACGAAAGACGCGTTTGGAGGAACCGGGATTGAAGGCGTTGAAGTTGCAGAATTTGCAAAGAGAAGAAGATGGTTTATCAGGAAGTGTTCTCTCACAGATAGAACAAACCCTTACAAGGACACGGACGAAAATCTACCATCTTTCAAATCATCTGGGCCGGCCCAGTCTCCGGATGGGCTTGATGTCAAAAAACGGCCCACTAATTCTACAGTGATTTCATCCGCCGGGAACGGAGGCGATGAAAAACCCAACGAATACAGGAGTCCTGAAATGCACGGAACTGGAGTCTCACTCTTCAAACGAAGAATTAAGAAGCTCCATCAATTTGGTTCAATTACCCTTTTGACTGTGAGGAAGTTGGAATTTGAGGTTCTTGCTATGGAGGCCGACCGGTTAAATTCTCCCAGTACATTTACCATGACCATGGAAGTATTGGGTCATGAAATGCGATTCTATCAGGATCCCAATTCGAAACATTTAGGTACGACAGTGTGGGATGCATCACTGGTATTTGTCAAGTTTCTGGAGAAAAATTGCCGAAAAGGAAAATTTAGCCCTTCTAAACTTAAAGGAAAGCGTGTCATTGAGCTTGGAGCTGGTTGTGGGTTGGCTGGGTTTGGCATGGCATTGCTTGGATGTGATGTAGTTGCTACAGACCAAAGGGAGGTATTGCCTATTCTAACGAGAAATATAGAACGTAATACACCAAGCTTAGCACAGATGAACCCTTCAGAATCATTTGGTTCAATTAGGGCAGCAGAATTAGACTGGGGAAATGAAGATCATATCAAGGCAGTAGGTCCACCATGTGACTATATTATCGGCACTGACGTGCGAATAAGTGGATTTTTGTTATTCAACGCGAAGGTTTATGCAGAACATCTATTGGAACCACTTTTAAAGACAATACATGCATTATCAGGTCCCAAAACTACAATTATGCTGGGGTATGAGATTCGTTCGACAAATGTTCACGAGCAAATGCTTGAGATGTGGAAAAAGAACTTTGAGGTGAAAACTGTATCACAATCAAAGATGGACTCACAGTATCAGCATCCCAGCATTCAGCTCTACATTATGGGGCTAAAGCCTGCACCTGGGAAAGCAGAGAACACTCGAGACAATGTTGAACAGGAAATTGACACGGTTTGTTCGAGGCAGGAGAATGATGAAAAGGAAGAAAGTTATGACGAAACGGGAATTGAGAAGGCAGAGGAGCCAGTTACAGAACATGAGGATAAGAAATTGGGTGACTGGGAAGCTAGAAGAATGGGCTCTATGGCCGCTCGGCTTCTGCAAGATGTAAAAATTGCTTGA

Protein sequence

MKFPSKKSGNTHKKTKDAFGGTGIEGVEVAEFAKRRRWFIRKCSLTDRTNPYKDTDENLPSFKSSGPAQSPDGLDVKKRPTNSTVISSAGNGGDEKPNEYRSPEMHGTGVSLFKRRIKKLHQFGSITLLTVRKLEFEVLAMEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSESFGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAPGKAENTRDNVEQEIDTVCSRQENDEKEESYDETGIEKAEEPVTEHEDKKLGDWEARRMGSMAARLLQDVKIA

Homology

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BLAST of HG10001336 vs. NCBI nr
Match: KAG6570813.1 (Protein-lysine methyltransferase METTL21D, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 554.7 bits (1428), Expect = 7.5e-154
Identity = 292/383 (76.24%), Postives = 313/383 (81.72%), Query Frame = 0


Query: 69  QSPDGLDVKKRPTNSTVISSAGNGGDEKPNEYRSPEMHGTGVSLFKRRIKKLHQFGSITL 128
           QSP G  V KRPT+  V SS G    + P      E  G         ++      SI L
Sbjct: 12  QSPAGPVVNKRPTDIMVASSPGTEAMKNPTNIGDLECTGLEPQSSNNELR-----SSINL 71

Query: 129 LTVRKLEFEVLAMEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKF 188
                    V AMEADRLNSPSTFTMTMEVLGHE+RFYQDPNSKHLGTTVWDASLVFVKF
Sbjct: 72  ---------VFAMEADRLNSPSTFTMTMEVLGHELRFYQDPNSKHLGTTVWDASLVFVKF 131

Query: 189 LEKNCRKGKFSPSKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERN 248
           LE+NCRKGKFSPSKLKGKRV+ELGAGCGLAGFGMALLGCDV+ATDQ+EVLPIL+RNIERN
Sbjct: 132 LERNCRKGKFSPSKLKGKRVVELGAGCGLAGFGMALLGCDVIATDQKEVLPILSRNIERN 191

Query: 249 TPSLAQMNPSESFGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEH 308
           TPSLAQMNPS+SFGSIRAAELDWGNEDHIKAVGPP D+IIGTDV           VYAEH
Sbjct: 192 TPSLAQMNPSDSFGSIRAAELDWGNEDHIKAVGPPFDFIIGTDV-----------VYAEH 251

Query: 309 LLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPS 368
           LLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVS+SKMDSQYQHPS
Sbjct: 252 LLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKSKMDSQYQHPS 311

Query: 369 IQLYIMGLKPAPGKAENTRDNVEQEIDTVCSRQENDEKEESYDETGIEKAEEPVTEHEDK 428
           IQLYIMG+K A   AENT+DNV+QEID VCS QENDEK+ S D TGI+K EEPV+E ED+
Sbjct: 312 IQLYIMGMKSAAENAENTQDNVDQEIDRVCSSQENDEKDVSCDGTGIQKVEEPVSEREDQ 369

Query: 429 KLGDWEARRMGSMAARLLQDVKI 452
           KLGDWEARR+GSMAARLLQDVKI
Sbjct: 372 KLGDWEARRIGSMAARLLQDVKI 369

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BLAST of HG10001336 vs. NCBI nr
Match: XP_008465352.1 (PREDICTED: protein N-lysine methyltransferase METTL21A [Cucumis melo] >XP_016903376.1 PREDICTED: protein N-lysine methyltransferase METTL21A [Cucumis melo] >XP_016903377.1 PREDICTED: protein N-lysine methyltransferase METTL21A [Cucumis melo])

HSP 1 Score: 550.8 bits (1418), Expect = 1.1e-152
Identity = 279/319 (87.46%), Postives = 291/319 (91.22%), Query Frame = 0


Query: 141 MEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 200
           MEADRLNSPSTFTMTMEVLGHE+RFYQDPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSP
Sbjct: 1   MEADRLNSPSTFTMTMEVLGHELRFYQDPNSKHLGTTVWDASLVFVKFLERNCRKGKFSP 60

Query: 201 SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSES 260
           SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPIL+RNIERNTPSLAQMNPS+S
Sbjct: 61  SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILSRNIERNTPSLAQMNPSDS 120

Query: 261 FGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHAL 320
           FGSI+AAELDWGNEDHIKAVGPP D+IIGTDV           VYAEHLLEPLLKTIHAL
Sbjct: 121 FGSIKAAELDWGNEDHIKAVGPPFDFIIGTDV-----------VYAEHLLEPLLKTIHAL 180

Query: 321 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAP 380
           SGPKTTIMLGYEIRSTNVHEQMLEMWK NFEVKTVSQSKMDSQYQHPSIQLYIMG+K   
Sbjct: 181 SGPKTTIMLGYEIRSTNVHEQMLEMWKTNFEVKTVSQSKMDSQYQHPSIQLYIMGIKSGA 240

Query: 381 GKAENTRDNVEQEIDTVCSRQEND--------EKEESYDETGIEKAEEPVTEHEDKKLGD 440
           G A+NT+ NVEQEIDTVCSRQEND        EK+ SYDETGI+K EEPVTEH+DKKLGD
Sbjct: 241 GNAKNTQGNVEQEIDTVCSRQENDETDVSFNNEKDVSYDETGIQKTEEPVTEHKDKKLGD 300

Query: 441 WEARRMGSMAARLLQDVKI 452
           WEARRMGSMAARLLQDVKI
Sbjct: 301 WEARRMGSMAARLLQDVKI 308

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BLAST of HG10001336 vs. NCBI nr
Match: XP_004145760.1 (protein N-lysine methyltransferase METTL21A [Cucumis sativus] >XP_031742643.1 protein N-lysine methyltransferase METTL21A [Cucumis sativus] >KGN46871.1 hypothetical protein Csa_020619 [Cucumis sativus])

HSP 1 Score: 548.1 bits (1411), Expect = 7.0e-152
Identity = 278/319 (87.15%), Postives = 290/319 (90.91%), Query Frame = 0


Query: 141 MEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 200
           MEADRLNSPSTFTMTMEVLGHE+RFYQDPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSP
Sbjct: 1   MEADRLNSPSTFTMTMEVLGHELRFYQDPNSKHLGTTVWDASLVFVKFLERNCRKGKFSP 60

Query: 201 SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSES 260
           SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPIL+RNIERNTPSLAQMNPS+S
Sbjct: 61  SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILSRNIERNTPSLAQMNPSDS 120

Query: 261 FGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHAL 320
           FGSIRAAELDWGNEDHIKAVGPP D+IIGTDV           VYAEHLLEPLL TIHAL
Sbjct: 121 FGSIRAAELDWGNEDHIKAVGPPFDFIIGTDV-----------VYAEHLLEPLLMTIHAL 180

Query: 321 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAP 380
           SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLK   
Sbjct: 181 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKSRA 240

Query: 381 GKAENTRDNVEQEIDTVCSRQENDEKEESY--------DETGIEKAEEPVTEHEDKKLGD 440
           G A+NT  NVEQEIDTVCSRQENDEK+ S+        DETG++K +EPVTEH+DKKLGD
Sbjct: 241 GNAKNTPGNVEQEIDTVCSRQENDEKDVSFNDEKDISCDETGVQKTDEPVTEHKDKKLGD 300

Query: 441 WEARRMGSMAARLLQDVKI 452
           WEARRMGSMAARLLQDVKI
Sbjct: 301 WEARRMGSMAARLLQDVKI 308

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BLAST of HG10001336 vs. NCBI nr
Match: XP_038901723.1 (protein N-lysine methyltransferase METTL21A [Benincasa hispida] >XP_038901724.1 protein N-lysine methyltransferase METTL21A [Benincasa hispida] >XP_038901725.1 protein N-lysine methyltransferase METTL21A [Benincasa hispida])

HSP 1 Score: 545.0 bits (1403), Expect = 5.9e-151
Identity = 275/312 (88.14%), Postives = 287/312 (91.99%), Query Frame = 0


Query: 141 MEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 200
           MEADRLNSPSTFTMTMEVLGHE+RFYQDPNSKHLGTTVWDASLVFVKFLEKNCR+GKFSP
Sbjct: 1   MEADRLNSPSTFTMTMEVLGHELRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRRGKFSP 60

Query: 201 SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSES 260
           SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQR+VLPIL RNIERNTPSLAQMNPS+S
Sbjct: 61  SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQRDVLPILARNIERNTPSLAQMNPSDS 120

Query: 261 FGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHAL 320
           FGSIRAAELDWGNE HI AVGPP D+IIGTDV           VYAEHLLEPLLKTIHAL
Sbjct: 121 FGSIRAAELDWGNEYHIMAVGPPFDFIIGTDV-----------VYAEHLLEPLLKTIHAL 180

Query: 321 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAP 380
           SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLK A 
Sbjct: 181 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKSAA 240

Query: 381 GKAENTRDNVEQEIDTVCSRQENDEKEESYDETGIEKAEEPVTEHEDKKLGDWEARRMGS 440
           G AENTRDN++QEIDTVCSRQEND+K+ SYDE  I++AEEPV   EDKKLG+WE RRMGS
Sbjct: 241 GNAENTRDNIQQEIDTVCSRQENDKKDVSYDEKEIQEAEEPVRGDEDKKLGNWETRRMGS 300

Query: 441 MAARLLQDVKIA 453
           MAARLLQDVKIA
Sbjct: 301 MAARLLQDVKIA 301

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BLAST of HG10001336 vs. NCBI nr
Match: XP_022944009.1 (protein-lysine methyltransferase METTL21D [Cucurbita moschata] >XP_022944011.1 protein-lysine methyltransferase METTL21D [Cucurbita moschata])

HSP 1 Score: 541.6 bits (1394), Expect = 6.5e-150
Identity = 271/311 (87.14%), Postives = 287/311 (92.28%), Query Frame = 0


Query: 141 MEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 200
           ME DRLNSPSTFTMTMEVLGHE+RFYQDPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSP
Sbjct: 1   METDRLNSPSTFTMTMEVLGHELRFYQDPNSKHLGTTVWDASLVFVKFLERNCRKGKFSP 60

Query: 201 SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSES 260
           SKLKGKRV+ELGAGCGLAGFGMALLGCDVVATDQ+EVLPIL+RNIERNTPSLAQMNPS+S
Sbjct: 61  SKLKGKRVVELGAGCGLAGFGMALLGCDVVATDQKEVLPILSRNIERNTPSLAQMNPSDS 120

Query: 261 FGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHAL 320
           FGSIRAAELDWGNEDHIKAVGPP D+IIGTDV           VYAEHLLEPLLKTIHAL
Sbjct: 121 FGSIRAAELDWGNEDHIKAVGPPFDFIIGTDV-----------VYAEHLLEPLLKTIHAL 180

Query: 321 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAP 380
           SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVS+SKMDSQYQHPSIQLYIMG+K A 
Sbjct: 181 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKSKMDSQYQHPSIQLYIMGMKSAA 240

Query: 381 GKAENTRDNVEQEIDTVCSRQENDEKEESYDETGIEKAEEPVTEHEDKKLGDWEARRMGS 440
             AENT+DNV+QEID VCS QENDEK+ S D TGI+K EEPV+E ED+KLGDWEARR+GS
Sbjct: 241 ENAENTQDNVDQEIDRVCSSQENDEKDVSCDGTGIQKVEEPVSEREDQKLGDWEARRIGS 300

Query: 441 MAARLLQDVKI 452
           MAARLLQDVKI
Sbjct: 301 MAARLLQDVKI 300

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BLAST of HG10001336 vs. ExPASy Swiss-Prot
Match: Q8C436 (Protein-lysine methyltransferase METTL21D OS=Mus musculus OX=10090 GN=Vcpkmt PE=2 SV=2)

HSP 1 Score: 100.5 bits (249), Expect = 5.1e-20
Identity = 74/219 (33.79%), Postives = 106/219 (48.40%), Query Frame = 0


Query: 167 QDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRVIELGAGCGLAGFGMALLG 226
           Q   S  +G  VWDA++V  K+LE     G      L  + V+ELG+G G  G   A LG
Sbjct: 31  QQYGSGGVGCVVWDAAIVLSKYLETPGFSGD-GAHALSRRSVLELGSGTGAVGLMAATLG 90

Query: 227 CDVVATDQREVLPILTRNIERNTPSLAQMNPSESFGSIRAAELDWGNEDHIKAVGPPCDY 286
            DV+ TD  E+  +L  NI+        MN     GS++A  L WG ED    + P  DY
Sbjct: 91  ADVIVTDLEELQDLLKMNID--------MNKHLVTGSVQAKVLKWG-EDIEDLMSP--DY 150

Query: 287 IIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHALSGPKTTIMLGYEIR----STNVHEQM 346
           I+  D            +Y E  LEPLLKT+  LSG +T I+  YE R    +  + ++ 
Sbjct: 151 ILMADC-----------IYYEESLEPLLKTLKDLSGSETCIICCYEQRTMGKNPEIEKKY 210

Query: 347 LEMWKKNFEVKTVSQSKMDSQYQHPSIQL-YIMGLKPAP 381
            E+ + +F+ + +   K D +Y+   I + YI   KP P
Sbjct: 211 FELLQLDFDFEEIPLDKHDEEYRSEDIHIVYIRKKKPKP 226

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BLAST of HG10001336 vs. ExPASy Swiss-Prot
Match: Q9H867 (Protein-lysine methyltransferase METTL21D OS=Homo sapiens OX=9606 GN=VCPKMT PE=1 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 1.2e-18
Identity = 68/209 (32.54%), Postives = 101/209 (48.33%), Query Frame = 0


Query: 167 QDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRVIELGAGCGLAGFGMALLG 226
           Q  +S  +G  VWDA++V  K+LE     G      L  + V+ELG+G G  G   A LG
Sbjct: 31  QQYSSGGVGCVVWDAAIVLSKYLETPEFSGD-GAHALSRRSVLELGSGTGAVGLMAATLG 90

Query: 227 CDVVATDQREVLPILTRNIERNTPSLAQMNPSESFGSIRAAELDWGNEDHIKAVGPPCDY 286
            DVV TD  E+  +L  NI         MN     GS++A  L WG E  I+    P D+
Sbjct: 91  ADVVVTDLEELQDLLKMNI--------NMNKHLVTGSVQAKVLKWGEE--IEGFPSPPDF 150

Query: 287 IIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHALSGPKTTIMLGYEIR----STNVHEQM 346
           I+  D            +Y E  LEPLLKT+  +SG +T I+  YE R    +  + ++ 
Sbjct: 151 ILMADC-----------IYYEESLEPLLKTLKDISGFETCIICCYEQRTMGKNPEIEKKY 210

Query: 347 LEMWKKNFEVKTVSQSKMDSQYQHPSIQL 372
            E+ + +F+ + +   K D +Y+   I +
Sbjct: 211 FELLQLDFDFEKIPLEKHDEEYRSEDIHI 217

[Close]

BLAST of HG10001336 vs. ExPASy Swiss-Prot
Match: Q5BLD8 (Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.6e-18
Identity = 72/208 (34.62%), Postives = 96/208 (46.15%), Query Frame = 0


Query: 148 SPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKR 207
           S + FT+      H +R  QD     +   VWDA++V   FLE     GK     LKGKR
Sbjct: 20  SSAEFTLA----NHRIRLSQDWKRLGVAAVVWDAAVVLCMFLE----MGKVD---LKGKR 79

Query: 208 VIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSESFGSIRAA 267
           VIELGAG GL G   ALLG +V  TD+   L  LT N+  N P   Q        +++ +
Sbjct: 80  VIELGAGTGLVGIVAALLGANVTITDREPALEFLTANVHENIPQGRQK-------AVQVS 139

Query: 268 ELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHALSGPKTTI 327
           EL WG    +   G   D I+G D+           VY E     LL+T+  LS   T +
Sbjct: 140 ELTWGENLDLYPQG-GYDLILGADI-----------VYLEETFPALLQTLEHLSSGDTVV 196

Query: 328 MLGYEIRSTNVHEQMLEMWKKNFEVKTV 356
           +L   IR     E+ L   ++ F V+ V
Sbjct: 200 LLSCRIRYER-DERFLTELRQRFSVQEV 196

[Close]

BLAST of HG10001336 vs. ExPASy Swiss-Prot
Match: A4IGU3 (Protein N-lysine methyltransferase METTL21A OS=Xenopus tropicalis OX=8364 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 2.1e-13
Identity = 59/205 (28.78%), Postives = 86/205 (41.95%), Query Frame = 0


Query: 155 TMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRVIELGAG 214
           + + + H +   QD     +   VWDA+LV   +LE            L+   VIELGAG
Sbjct: 23  SFKFVNHNIEIKQDWKQLGVAAVVWDAALVLCMYLES-------EGIHLQNSSVIELGAG 82

Query: 215 CGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSESFGSIRAAELDWGNE 274
            GL G   ALLG  V  TD+   +  L  N+  N        P +S   +    L+WG  
Sbjct: 83  TGLVGIVAALLGAQVTITDRDLAMEFLRMNVRDNI-------PKDSLHRVSVRALNWGKS 142

Query: 275 DHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHALSGPKTTIMLGYEIR 334
               +     D+I+G D+           +Y E     LL+T   LS  ++ I+L   +R
Sbjct: 143 LEEFST---YDFILGADI-----------IYLEETFPDLLQTFLHLSSQQSVILLSSRLR 198

Query: 335 STNVHEQMLEMWKKNFEVKTVSQSK 360
               H+  LEM K +F +  V   K
Sbjct: 203 YQRDHD-FLEMMKLHFTIADVYYDK 198

[Close]

BLAST of HG10001336 vs. ExPASy Swiss-Prot
Match: Q8WXB1 (Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 7.8e-13
Identity = 58/202 (28.71%), Postives = 85/202 (42.08%), Query Frame = 0


Query: 155 TMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRVIELGAG 214
           T     H ++  QD     +   VWDA++V   +LE           +L+G+  +ELGAG
Sbjct: 23  TFSFANHTIQIRQDWRHLGVAAVVWDAAIVLSTYLE-------MGAVELRGRSAVELGAG 82

Query: 215 CGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSESFGSIRAAELDWGNE 274
            GL G   ALLG  V  TD++  L  L  N++ N P   Q             EL WG  
Sbjct: 83  TGLVGIVAALLGAHVTITDRKVALEFLKSNVQANLPPHIQTKTV-------VKELTWG-- 142

Query: 275 DHIKAVGP-PCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHALSGPKTTIMLGYEI 334
            ++ +  P   D I+G D+           +Y E     LL+T+  L    + I+L   I
Sbjct: 143 QNLGSFSPGEFDLILGADI-----------IYLEETFTDLLQTLEHLCSNHSVILLACRI 196

Query: 335 RSTNVHEQMLEMWKKNFEVKTV 356
           R        L M ++ F V+ V
Sbjct: 203 RYER-DNNFLAMLERQFTVRKV 196

[Close]

BLAST of HG10001336 vs. ExPASy TrEMBL
Match: A0A1S4E579 (protein N-lysine methyltransferase METTL21A OS=Cucumis melo OX=3656 GN=LOC103502991 PE=4 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 5.2e-153
Identity = 279/319 (87.46%), Postives = 291/319 (91.22%), Query Frame = 0


Query: 141 MEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 200
           MEADRLNSPSTFTMTMEVLGHE+RFYQDPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSP
Sbjct: 1   MEADRLNSPSTFTMTMEVLGHELRFYQDPNSKHLGTTVWDASLVFVKFLERNCRKGKFSP 60

Query: 201 SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSES 260
           SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPIL+RNIERNTPSLAQMNPS+S
Sbjct: 61  SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILSRNIERNTPSLAQMNPSDS 120

Query: 261 FGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHAL 320
           FGSI+AAELDWGNEDHIKAVGPP D+IIGTDV           VYAEHLLEPLLKTIHAL
Sbjct: 121 FGSIKAAELDWGNEDHIKAVGPPFDFIIGTDV-----------VYAEHLLEPLLKTIHAL 180

Query: 321 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAP 380
           SGPKTTIMLGYEIRSTNVHEQMLEMWK NFEVKTVSQSKMDSQYQHPSIQLYIMG+K   
Sbjct: 181 SGPKTTIMLGYEIRSTNVHEQMLEMWKTNFEVKTVSQSKMDSQYQHPSIQLYIMGIKSGA 240

Query: 381 GKAENTRDNVEQEIDTVCSRQEND--------EKEESYDETGIEKAEEPVTEHEDKKLGD 440
           G A+NT+ NVEQEIDTVCSRQEND        EK+ SYDETGI+K EEPVTEH+DKKLGD
Sbjct: 241 GNAKNTQGNVEQEIDTVCSRQENDETDVSFNNEKDVSYDETGIQKTEEPVTEHKDKKLGD 300

Query: 441 WEARRMGSMAARLLQDVKI 452
           WEARRMGSMAARLLQDVKI
Sbjct: 301 WEARRMGSMAARLLQDVKI 308

[Close]

BLAST of HG10001336 vs. ExPASy TrEMBL
Match: A0A0A0KGI7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G147640 PE=4 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 3.4e-152
Identity = 278/319 (87.15%), Postives = 290/319 (90.91%), Query Frame = 0


Query: 141 MEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 200
           MEADRLNSPSTFTMTMEVLGHE+RFYQDPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSP
Sbjct: 1   MEADRLNSPSTFTMTMEVLGHELRFYQDPNSKHLGTTVWDASLVFVKFLERNCRKGKFSP 60

Query: 201 SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSES 260
           SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPIL+RNIERNTPSLAQMNPS+S
Sbjct: 61  SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILSRNIERNTPSLAQMNPSDS 120

Query: 261 FGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHAL 320
           FGSIRAAELDWGNEDHIKAVGPP D+IIGTDV           VYAEHLLEPLL TIHAL
Sbjct: 121 FGSIRAAELDWGNEDHIKAVGPPFDFIIGTDV-----------VYAEHLLEPLLMTIHAL 180

Query: 321 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAP 380
           SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLK   
Sbjct: 181 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKSRA 240

Query: 381 GKAENTRDNVEQEIDTVCSRQENDEKEESY--------DETGIEKAEEPVTEHEDKKLGD 440
           G A+NT  NVEQEIDTVCSRQENDEK+ S+        DETG++K +EPVTEH+DKKLGD
Sbjct: 241 GNAKNTPGNVEQEIDTVCSRQENDEKDVSFNDEKDISCDETGVQKTDEPVTEHKDKKLGD 300

Query: 441 WEARRMGSMAARLLQDVKI 452
           WEARRMGSMAARLLQDVKI
Sbjct: 301 WEARRMGSMAARLLQDVKI 308

[Close]

BLAST of HG10001336 vs. ExPASy TrEMBL
Match: A0A6J1FT98 (protein-lysine methyltransferase METTL21D OS=Cucurbita moschata OX=3662 GN=LOC111448568 PE=4 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 3.2e-150
Identity = 271/311 (87.14%), Postives = 287/311 (92.28%), Query Frame = 0


Query: 141 MEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 200
           ME DRLNSPSTFTMTMEVLGHE+RFYQDPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSP
Sbjct: 1   METDRLNSPSTFTMTMEVLGHELRFYQDPNSKHLGTTVWDASLVFVKFLERNCRKGKFSP 60

Query: 201 SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSES 260
           SKLKGKRV+ELGAGCGLAGFGMALLGCDVVATDQ+EVLPIL+RNIERNTPSLAQMNPS+S
Sbjct: 61  SKLKGKRVVELGAGCGLAGFGMALLGCDVVATDQKEVLPILSRNIERNTPSLAQMNPSDS 120

Query: 261 FGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHAL 320
           FGSIRAAELDWGNEDHIKAVGPP D+IIGTDV           VYAEHLLEPLLKTIHAL
Sbjct: 121 FGSIRAAELDWGNEDHIKAVGPPFDFIIGTDV-----------VYAEHLLEPLLKTIHAL 180

Query: 321 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAP 380
           SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVS+SKMDSQYQHPSIQLYIMG+K A 
Sbjct: 181 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKSKMDSQYQHPSIQLYIMGMKSAA 240

Query: 381 GKAENTRDNVEQEIDTVCSRQENDEKEESYDETGIEKAEEPVTEHEDKKLGDWEARRMGS 440
             AENT+DNV+QEID VCS QENDEK+ S D TGI+K EEPV+E ED+KLGDWEARR+GS
Sbjct: 241 ENAENTQDNVDQEIDRVCSSQENDEKDVSCDGTGIQKVEEPVSEREDQKLGDWEARRIGS 300

Query: 441 MAARLLQDVKI 452
           MAARLLQDVKI
Sbjct: 301 MAARLLQDVKI 300

[Close]

BLAST of HG10001336 vs. ExPASy TrEMBL
Match: A0A6J1CGP5 (protein N-lysine methyltransferase METTL21A OS=Momordica charantia OX=3673 GN=LOC111011074 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.8e-145
Identity = 265/313 (84.66%), Postives = 284/313 (90.73%), Query Frame = 0


Query: 141 MEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 200
           MEADRLNSPSTFTMTMEVLGHE+RFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP
Sbjct: 1   MEADRLNSPSTFTMTMEVLGHELRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 60

Query: 201 SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSES 260
           SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQ+EVLPILTRNIERNTPS AQMNPS+S
Sbjct: 61  SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQKEVLPILTRNIERNTPSFAQMNPSDS 120

Query: 261 FGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHAL 320
           FGSI AAELDWGNEDHIKAVGPP D+IIGTDV           VYAEHLLEPLL TIHAL
Sbjct: 121 FGSISAAELDWGNEDHIKAVGPPFDFIIGTDV-----------VYAEHLLEPLLMTIHAL 180

Query: 321 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAP 380
           SGPKTTIMLGYEIRSTNVHEQML+MWKKNFEVKTVS+SKMDSQYQHP+IQLYIMGLK A 
Sbjct: 181 SGPKTTIMLGYEIRSTNVHEQMLDMWKKNFEVKTVSKSKMDSQYQHPNIQLYIMGLKSAA 240

Query: 381 GKAENTR-DNVEQEIDTVCSRQENDEKEESYDETGIEKAEEPVTEHEDKKLGDWEARRMG 440
           G  EN + DN++Q+ID VCS +END+K+ S DET I+KAEEPVTE+ D+K+ DWE RR+G
Sbjct: 241 GNTENPQEDNIDQKIDRVCSSRENDKKDVSCDETDIQKAEEPVTENADEKVSDWEVRRIG 300

Query: 441 SMAARLLQDVKIA 453
           SMAARLLQDVKIA
Sbjct: 301 SMAARLLQDVKIA 302

[Close]

BLAST of HG10001336 vs. ExPASy TrEMBL
Match: A0A6J1JBP5 (protein N-lysine methyltransferase METTL21A OS=Cucurbita maxima OX=3661 GN=LOC111483605 PE=4 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 7.6e-144
Identity = 264/311 (84.89%), Postives = 281/311 (90.35%), Query Frame = 0


Query: 141 MEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 200
           ME DRLNSPSTFTMTMEVLGHE+RFYQDPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSP
Sbjct: 1   METDRLNSPSTFTMTMEVLGHELRFYQDPNSKHLGTTVWDASLVFVKFLERNCRKGKFSP 60

Query: 201 SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSES 260
           SKLKGKRV+ELGAGCGLAGFGMALLGCDVVATDQ+EVLPIL+RNIERNTPSLAQMNPS+S
Sbjct: 61  SKLKGKRVVELGAGCGLAGFGMALLGCDVVATDQKEVLPILSRNIERNTPSLAQMNPSDS 120

Query: 261 FGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHAL 320
           FGSIRAAELDWGNEDHIKAVGPP D+IIGTDV           VYAEHLLEPLLKTIHAL
Sbjct: 121 FGSIRAAELDWGNEDHIKAVGPPFDFIIGTDV-----------VYAEHLLEPLLKTIHAL 180

Query: 321 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAP 380
           SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVS+SKMDSQYQHPSIQLYIM LK A 
Sbjct: 181 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKSKMDSQYQHPSIQLYIMVLKSAA 240

Query: 381 GKAENTRDNVEQEIDTVCSRQENDEKEESYDETGIEKAEEPVTEHEDKKLGDWEARRMGS 440
             AE TR+NV+QEID V   QE DEK+ S D  GI+KA+EPV+  ED+KLGDWEARR+GS
Sbjct: 241 ENAEKTRENVDQEIDRVFLSQEIDEKDVSCDGKGIQKADEPVSGREDQKLGDWEARRIGS 300

Query: 441 MAARLLQDVKI 452
           +AARLLQDVKI
Sbjct: 301 VAARLLQDVKI 300

[Close]

BLAST of HG10001336 vs. TAIR 10
Match: AT1G08125.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 368.6 bits (945), Expect = 7.1e-102
Identity = 197/320 (61.56%), Postives = 235/320 (73.44%), Query Frame = 0


Query: 141 MEADRLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSP 200
           M+ DRLNSPST T+T+EVLGHE+ F QDPNSKHLGTTVWDAS+VF K+L KN RKG+FS 
Sbjct: 1   MDPDRLNSPSTCTVTIEVLGHELDFAQDPNSKHLGTTVWDASMVFAKYLGKNSRKGRFSS 60

Query: 201 SKLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSES 260
           SKLKGKR IELGAGCG+AGF +A+LGCDVV TDQ+EVLP+L RN+E NT  + QMNP  +
Sbjct: 61  SKLKGKRAIELGAGCGVAGFALAMLGCDVVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSA 120

Query: 261 FGSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHAL 320
           FGS+R AELDWGNEDHI AV PP DY+IGTDV           VY+E LLEPLL+TI AL
Sbjct: 121 FGSLRVAELDWGNEDHITAVEPPFDYVIGTDV-----------VYSEQLLEPLLRTILAL 180

Query: 321 SGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAP 380
           SGPKTT+MLGYEIRST VHE+ML+MWK NFEVKT+ +SKMD +YQ PSI LYIM  K A 
Sbjct: 181 SGPKTTVMLGYEIRSTVVHEKMLQMWKDNFEVKTIPRSKMDGEYQDPSIHLYIMAQKSAA 240

Query: 381 GKAEN-TRDNVEQEID-TVCSRQ------ENDEKEESYDETGIEKAEEPVTEH-EDKKLG 440
             + N +RD V  + D T C  +          +EE+    G    E+ +     D KL 
Sbjct: 241 ESSGNVSRDEVVIDTDETKCETKVPTGECHKRVEEEAVVTHGPRLNEDSLLMRLRDGKLS 300

Query: 441 DWEARRMGSMAARLLQDVKI 452
           +WE RR G++AA+LL+DVKI
Sbjct: 301 EWEMRRYGTVAAQLLRDVKI 309

[Close]

BLAST of HG10001336 vs. TAIR 10
Match: AT1G08125.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 364.0 bits (933), Expect = 1.7e-100
Identity = 195/316 (61.71%), Postives = 232/316 (73.42%), Query Frame = 0


Query: 145 RLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLK 204
           RLNSPST T+T+EVLGHE+ F QDPNSKHLGTTVWDAS+VF K+L KN RKG+FS SKLK
Sbjct: 12  RLNSPSTCTVTIEVLGHELDFAQDPNSKHLGTTVWDASMVFAKYLGKNSRKGRFSSSKLK 71

Query: 205 GKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSESFGSI 264
           GKR IELGAGCG+AGF +A+LGCDVV TDQ+EVLP+L RN+E NT  + QMNP  +FGS+
Sbjct: 72  GKRAIELGAGCGVAGFALAMLGCDVVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSL 131

Query: 265 RAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHALSGPK 324
           R AELDWGNEDHI AV PP DY+IGTDV           VY+E LLEPLL+TI ALSGPK
Sbjct: 132 RVAELDWGNEDHITAVEPPFDYVIGTDV-----------VYSEQLLEPLLRTILALSGPK 191

Query: 325 TTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAPGKAE 384
           TT+MLGYEIRST VHE+ML+MWK NFEVKT+ +SKMD +YQ PSI LYIM  K A   + 
Sbjct: 192 TTVMLGYEIRSTVVHEKMLQMWKDNFEVKTIPRSKMDGEYQDPSIHLYIMAQKSAAESSG 251

Query: 385 N-TRDNVEQEID-TVCSRQ------ENDEKEESYDETGIEKAEEPVTEH-EDKKLGDWEA 444
           N +RD V  + D T C  +          +EE+    G    E+ +     D KL +WE 
Sbjct: 252 NVSRDEVVIDTDETKCETKVPTGECHKRVEEEAVVTHGPRLNEDSLLMRLRDGKLSEWEM 311

Query: 445 RRMGSMAARLLQDVKI 452
           RR G++AA+LL+DVKI
Sbjct: 312 RRYGTVAAQLLRDVKI 316

[Close]

BLAST of HG10001336 vs. TAIR 10
Match: AT1G08125.3 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 243.4 bits (620), Expect = 3.4e-64
Identity = 151/316 (47.78%), Postives = 181/316 (57.28%), Query Frame = 0


Query: 145 RLNSPSTFTMTMEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLK 204
           RLNSPST T+T+EVLGHE+ F QDPNSKHLGTTVWDAS+VF K+L KN RKG+FS SKLK
Sbjct: 29  RLNSPSTCTVTIEVLGHELDFAQDPNSKHLGTTVWDASMVFAKYLGKNSRKGRFSSSKLK 88

Query: 205 GKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSESFGSI 264
           GKR IELGAGCG+AGF +A+LGCDV                                   
Sbjct: 89  GKRAIELGAGCGVAGFALAMLGCDV----------------------------------- 148

Query: 265 RAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHALSGPK 324
                                                  VY+E LLEPLL+TI ALSGPK
Sbjct: 149 ---------------------------------------VYSEQLLEPLLRTILALSGPK 208

Query: 325 TTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAPGKAE 384
           TT+MLGYEIRST VHE+ML+MWK NFEVKT+ +SKMD +YQ PSI LYIM  K A   + 
Sbjct: 209 TTVMLGYEIRSTVVHEKMLQMWKDNFEVKTIPRSKMDGEYQDPSIHLYIMAQKSAAESSG 268

Query: 385 N-TRDNVEQEID-TVCSRQ------ENDEKEESYDETGIEKAEEPVTEH-EDKKLGDWEA 444
           N +RD V  + D T C  +          +EE+    G    E+ +     D KL +WE 
Sbjct: 269 NVSRDEVVIDTDETKCETKVPTGECHKRVEEEAVVTHGPRLNEDSLLMRLRDGKLSEWEM 270

Query: 445 RRMGSMAARLLQDVKI 452
           RR G++AA+LL+DVKI
Sbjct: 329 RRYGTVAAQLLRDVKI 270

[Close]

BLAST of HG10001336 vs. TAIR 10
Match: AT1G73320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 1.7e-26
Identity = 75/236 (31.78%), Postives = 122/236 (51.69%), Query Frame = 0


Query: 146 LNSPSTFTMTMEVLGHEMRFYQDPNSKH----LGTTVWDASLVFVKFLEKNCRKGKFSPS 205
           L + S+  + ++  GH +   Q P S +     G+ +WD+ +V  KFLE +      S  
Sbjct: 86  LVAQSSLELRLDACGHSLSILQSPCSLNTPGVTGSVMWDSGVVLGKFLEHSVDSKVLS-- 145

Query: 206 KLKGKRVIELGAGCGLAGFGMALLGCDVVATDQREVLPILTRNIERNTPSLAQMNPSESF 265
            L+GK+++ELG+GCGL G   ALLG + V TD  + L +L +NI+ N      ++   + 
Sbjct: 146 -LEGKKIVELGSGCGLVGCIAALLGGNAVLTDLPDRLRLLKKNIQTN------LHRGNTR 205

Query: 266 GSIRAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHALS 325
           GS    EL WG++     + P  DY++G+DV           +Y+E  +  L+KT+  L 
Sbjct: 206 GSAIVQELVWGDDPDPDLIEPFPDYVLGSDV-----------IYSEEAVHHLVKTLLQLC 265

Query: 326 GPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLK 378
             +TTI L  E+R+  V E  LE   K+F +  V Q++    Y+   + LY++  K
Sbjct: 266 SDQTTIFLSGELRNDAVLEYFLETALKDFAIGRVEQTQWHPDYRSHRVVLYVLEKK 301

[Close]

BLAST of HG10001336 vs. TAIR 10
Match: AT5G44170.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 112.8 bits (281), Expect = 7.0e-25
Identity = 73/241 (30.29%), Postives = 114/241 (47.30%), Query Frame = 0


Query: 156 MEVLGHEMRFYQDPNSKHLGTTVWDASLVFVKFLEK----NCRKGKFSPS------KLKG 215
           + V G ++   QD  S H+GT+VW  SL+  KF E+    +      SP+        + 
Sbjct: 11  LTVNGTKLSIQQDNGSMHVGTSVWPCSLILSKFAERWSTLDSSSSTTSPNPYAELFDFRR 70

Query: 216 KRVIELGAGCGLAGFGMALLG-CDVVATDQREVLPILTRNIERNTPSLAQMNPSESFGSI 275
           +R IELG GCG+AG    LLG  ++V TD   V+P L  N++RN  +L +        S+
Sbjct: 71  RRGIELGTGCGVAGMAFYLLGLTEIVLTDIAPVMPALKHNLKRNKTALGK--------SL 130

Query: 276 RAAELDWGNEDHIKAVGPPCDYIIGTDVRISGFLLFNAKVYAEHLLEPLLKTIHALSGPK 335
           + + + W N D I A+ PP D +I  DV           VY E  +  L+  +  L    
Sbjct: 131 KTSIVYWNNRDQISALKPPFDLVIAADV-----------VYIEESVGQLVTAMELLVADD 190

Query: 336 TTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQSKMDSQYQHPSIQLYIMGLKPAPGKAE 386
             ++LGY+IRS    +   E+    F+++ V    + S Y +    +YI   K    +AE
Sbjct: 191 GAVLLGYQIRSPEADKLFWELCDIVFKIEKVPHEHLHSDYAYEETDVYIFRKKVKKNEAE 232

The following BLAST results are available for this feature:

BLAST of HG10001336 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6570813.1	7.5e-154	76.24	Protein-lysine methyltransferase METTL21D, partial [Cucurbita argyrosperma subsp...	[more]
XP_008465352.1	1.1e-152	87.46	PREDICTED: protein N-lysine methyltransferase METTL21A [Cucumis melo] >XP_016903...	[more]
XP_004145760.1	7.0e-152	87.15	protein N-lysine methyltransferase METTL21A [Cucumis sativus] >XP_031742643.1 pr...	[more]
XP_038901723.1	5.9e-151	88.14	protein N-lysine methyltransferase METTL21A [Benincasa hispida] >XP_038901724.1 ...	[more]
XP_022944009.1	6.5e-150	87.14	protein-lysine methyltransferase METTL21D [Cucurbita moschata] >XP_022944011.1 p...	[more]

BLAST of HG10001336 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8C436	5.1e-20	33.79	Protein-lysine methyltransferase METTL21D OS=Mus musculus OX=10090 GN=Vcpkmt PE=...	[more]
Q9H867	1.2e-18	32.54	Protein-lysine methyltransferase METTL21D OS=Homo sapiens OX=9606 GN=VCPKMT PE=1...	[more]
Q5BLD8	1.6e-18	34.62	Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a P...	[more]
A4IGU3	2.1e-13	28.78	Protein N-lysine methyltransferase METTL21A OS=Xenopus tropicalis OX=8364 GN=met...	[more]
Q8WXB1	7.8e-13	28.71	Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A ...	[more]

BLAST of HG10001336 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E579	5.2e-153	87.46	protein N-lysine methyltransferase METTL21A OS=Cucumis melo OX=3656 GN=LOC103502...	[more]
A0A0A0KGI7	3.4e-152	87.15	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G147640 PE=4 SV=1	[more]
A0A6J1FT98	3.2e-150	87.14	protein-lysine methyltransferase METTL21D OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1CGP5	1.8e-145	84.66	protein N-lysine methyltransferase METTL21A OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1JBP5	7.6e-144	84.89	protein N-lysine methyltransferase METTL21A OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]

BLAST of HG10001336 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08125.1	7.1e-102	61.56	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G08125.2	1.7e-100	61.71	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G08125.3	3.4e-64	47.78	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G73320.1	1.7e-26	31.78	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G44170.1	7.0e-25	30.29	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 149..379 e-value: 1.8E-56 score: 193.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 397..431
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 76..91
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 50..105
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR14614:SF98	S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEIN	coord: 141..398
IPR019410	Lysine methyltransferase	PFAM	PF10294	Methyltransf_16	coord: 163..340 e-value: 1.1E-26 score: 93.6
IPR019410	Lysine methyltransferase	PANTHER	PTHR14614	HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN	coord: 141..398
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 202..350

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10001336.1	HG10001336.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity