HG10000217 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000217
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAPO protein 4, mitochondrial
LocationChr09: 2320934 .. 2322341 (+)
RNA-Seq ExpressionHG10000217
SyntenyHG10000217
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAACCATGGCTTTCATGGCTCTTAGAAGGAATTTCAGTGAGAATGTTGTGCAGGAATTCATGCTACAGCGGTGCTACAGCTCTAAAGTTGACTTGAAGAAGCTCCGTCCAATGATTTTGAAGAGAATTCAAGATCGGGCAAAAAACTATCCTATTAAGGGTATGACTCCAGTGGCACAGCAAGTCCTTGAAGCTAGGACAACGCTTATACATGGAGTTTCCACTCTTCTCAAATCATTTCCGGTTCTCTCTTGCAAGTTAGTATCCCGGGAACAAACACCCTCTAATTTCTCTTCTTTTTTATTTTTGTTATCATGTTATTCTGCTTCCTTTTAGTTTACTTTCTCTTTAAATAGTTTTTATCTAAATTGCATATTCCTTGAACTAGTATGTGTATTAGGGTATTACCATTTTGTCATAAATGCTGAGTGACCTTGATTTTCAGTAAGCTGTTTCCTGACAAGTAGCCGATATAATAAATTTCCTGCCCTTGAAGTGAGGTGAATGAATTCAAAAATATATATTATCTAGCTCTTTATTCCCTCTAGGACCATCTGTAAAGTCTGTTCTTTCTAGAATTTAGATTGAACCATCCTAAGGTGTTAATATGAATGCATGTTTGTGAATGCTGTTTCTGAAACTCCTAGGATCATTGTATTATTTTTGTAGATTTTGTCCAGAAGTATATGTTGGTGAGAAAGGTCATCAAATACGAAGTTGTGGCGGTTACAAACGCGGTGCCAAGAATCAGGTTCATGAGTGGATCAGAGGTGATTTGAATGATATCATTGTCCCAGTAGAAGCATTTCACCTGCATCACATGTTCCAAGATGTTATTAAACATGATGAGAGGTTCAACTTTGAACGTGTTCCAGCAGTTGTCGAGCTATGCTGGCAAGCTGGAGCCAATCCAAATGATGAAAATCTGTCTTCAAGCACTAGGAATTCAGCTGAAGGAGGTGGCTCAGGCATGGATGAACAACCTTTGTCAGGTCGTGAGATGATGCTTTTAGCAACTGAAACTATCAGGGCATGGGAAACACTTCGAACCGGCGTGCAGAAGCTGTTGATGGTATATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTCTGTGGAGTTTTTAAATATGAGAATTGGCGAGGGTCCCATTTCTGGGAGAAGGCAGACGTAGATGATTTAGTTCCTCCAAAGATCGTATGGCATCGGCGACCACAAGATCCTCCTGTGCTTGTCGACGAAGGGAGGGATTGTTATGGACACACGCCAGCAGTGCTGGCTCTTTGCATACAAGCAGGAGCCCTTGCACCATCTAAGTATCACTGTATGATGAAAGTTCAAGGCTTACCACCTCGGAGTCAACTCAAGCTATGA

mRNA sequence

ATGAAAACCATGGCTTTCATGGCTCTTAGAAGGAATTTCAGTGAGAATGTTGTGCAGGAATTCATGCTACAGCGGTGCTACAGCTCTAAAGTTGACTTGAAGAAGCTCCGTCCAATGATTTTGAAGAGAATTCAAGATCGGGCAAAAAACTATCCTATTAAGGGTATGACTCCAGTGGCACAGCAAGTCCTTGAAGCTAGGACAACGCTTATACATGGAGTTTCCACTCTTCTCAAATCATTTCCGGTTCTCTCTTGCAAATTTTGTCCAGAAGTATATGTTGGTGAGAAAGGTCATCAAATACGAAGTTGTGGCGGTTACAAACGCGGTGCCAAGAATCAGGTTCATGAGTGGATCAGAGGTGATTTGAATGATATCATTGTCCCAGTAGAAGCATTTCACCTGCATCACATGTTCCAAGATGTTATTAAACATGATGAGAGGTTCAACTTTGAACGTGTTCCAGCAGTTGTCGAGCTATGCTGGCAAGCTGGAGCCAATCCAAATGATGAAAATCTGTCTTCAAGCACTAGGAATTCAGCTGAAGGAGGTGGCTCAGGCATGGATGAACAACCTTTGTCAGGTCGTGAGATGATGCTTTTAGCAACTGAAACTATCAGGGCATGGGAAACACTTCGAACCGGCGTGCAGAAGCTGTTGATGGTATATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTCTGTGGAGTTTTTAAATATGAGAATTGGCGAGGGTCCCATTTCTGGGAGAAGGCAGACGTAGATGATTTAGTTCCTCCAAAGATCGTATGGCATCGGCGACCACAAGATCCTCCTGTGCTTGTCGACGAAGGGAGGGATTGTTATGGACACACGCCAGCAGTGCTGGCTCTTTGCATACAAGCAGGAGCCCTTGCACCATCTAAGTATCACTGTATGATGAAAGTTCAAGGCTTACCACCTCGGAGTCAACTCAAGCTATGA

Coding sequence (CDS)

ATGAAAACCATGGCTTTCATGGCTCTTAGAAGGAATTTCAGTGAGAATGTTGTGCAGGAATTCATGCTACAGCGGTGCTACAGCTCTAAAGTTGACTTGAAGAAGCTCCGTCCAATGATTTTGAAGAGAATTCAAGATCGGGCAAAAAACTATCCTATTAAGGGTATGACTCCAGTGGCACAGCAAGTCCTTGAAGCTAGGACAACGCTTATACATGGAGTTTCCACTCTTCTCAAATCATTTCCGGTTCTCTCTTGCAAATTTTGTCCAGAAGTATATGTTGGTGAGAAAGGTCATCAAATACGAAGTTGTGGCGGTTACAAACGCGGTGCCAAGAATCAGGTTCATGAGTGGATCAGAGGTGATTTGAATGATATCATTGTCCCAGTAGAAGCATTTCACCTGCATCACATGTTCCAAGATGTTATTAAACATGATGAGAGGTTCAACTTTGAACGTGTTCCAGCAGTTGTCGAGCTATGCTGGCAAGCTGGAGCCAATCCAAATGATGAAAATCTGTCTTCAAGCACTAGGAATTCAGCTGAAGGAGGTGGCTCAGGCATGGATGAACAACCTTTGTCAGGTCGTGAGATGATGCTTTTAGCAACTGAAACTATCAGGGCATGGGAAACACTTCGAACCGGCGTGCAGAAGCTGTTGATGGTATATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTCTGTGGAGTTTTTAAATATGAGAATTGGCGAGGGTCCCATTTCTGGGAGAAGGCAGACGTAGATGATTTAGTTCCTCCAAAGATCGTATGGCATCGGCGACCACAAGATCCTCCTGTGCTTGTCGACGAAGGGAGGGATTGTTATGGACACACGCCAGCAGTGCTGGCTCTTTGCATACAAGCAGGAGCCCTTGCACCATCTAAGTATCACTGTATGATGAAAGTTCAAGGCTTACCACCTCGGAGTCAACTCAAGCTATGA

Protein sequence

MKTMAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVAQQVLEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIRGDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNSAEGGGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVLALCIQAGALAPSKYHCMMKVQGLPPRSQLKL
Homology
BLAST of HG10000217 vs. NCBI nr
Match: XP_038901944.1 (APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901946.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901947.1 APO protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 634.8 bits (1636), Expect = 4.2e-178
Identity = 304/331 (91.84%), Postives = 316/331 (95.47%), Query Frame = 0

Query: 1   MKTMAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMAL+R F E VVQEF+L RCYSSKV+LKKLRPMILKRIQ+R KNYPI+GMTPVA
Sbjct: 1   MKTMAFMALKRKFREIVVQEFVLHRCYSSKVNLKKLRPMILKRIQNREKNYPIRGMTPVA 60

Query: 61  QQVLEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIR 120
           QQVLEART LIHGVSTLLKSFPVLSCKFCPEVYVGEKGH IRSCGGYKRGAKNQVHEWIR
Sbjct: 61  QQVLEARTMLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHLIRSCGGYKRGAKNQVHEWIR 120

Query: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNS 180
           GDLNDIIVPVE+FHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDEN SSSTRNS
Sbjct: 121 GDLNDIIVPVESFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENQSSSTRNS 180

Query: 181 AEGGGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 240
            EGGGSG D +PLSG EMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG
Sbjct: 181 GEGGGSGRD-KPLSGHEMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 240

Query: 241 HKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVL 300
           HKARLCGVFKYE+WRGSHFWEKAD+DDLVPPK+VWHRRPQDPPVL+DEGRD YGHTPAVL
Sbjct: 241 HKARLCGVFKYEHWRGSHFWEKADIDDLVPPKLVWHRRPQDPPVLIDEGRDYYGHTPAVL 300

Query: 301 ALCIQAGALAPSKYHCMMKVQGLPPRSQLKL 332
           ALC QAGALAPSKYHCMMKVQG+ PRSQLKL
Sbjct: 301 ALCTQAGALAPSKYHCMMKVQGISPRSQLKL 330

BLAST of HG10000217 vs. NCBI nr
Match: XP_004151879.1 (APO protein 4, mitochondrial [Cucumis sativus] >XP_031737269.1 APO protein 4, mitochondrial [Cucumis sativus] >XP_031737270.1 APO protein 4, mitochondrial [Cucumis sativus] >KGN63269.1 hypothetical protein Csa_022178 [Cucumis sativus])

HSP 1 Score: 613.2 bits (1580), Expect = 1.3e-171
Identity = 295/331 (89.12%), Postives = 311/331 (93.96%), Query Frame = 0

Query: 1   MKTMAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMA+RR F +NVVQEFMLQRCYSSKV+LKKLRPMILKRIQDRAKNYPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 61  QQVLEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIR 120
           QQVLEAR  LIHGVSTLLKSFPVLSCKFCPEVYVGE+GH IRSCGGYKRGAKNQVH+WIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNS 180
           GDL DIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAGANP+D+NL+SST+NS
Sbjct: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180

Query: 181 AEGGGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 240
           AEGGGSGMDE PLS  EMMLLATETIRAWETLRTGVQKLLMVYP KVCKYCSEVHVGPSG
Sbjct: 181 AEGGGSGMDE-PLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSG 240

Query: 241 HKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVL 300
           HKARLCGVF YE+WRGSHFWEKADVDDLVPPKIVWHRR QDPPVLVD+G+D YGH PAV+
Sbjct: 241 HKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVV 300

Query: 301 ALCIQAGALAPSKYHCMMKVQGLPPRSQLKL 332
           ALC QAG +AP KYHCMMKVQGL PR +L+L
Sbjct: 301 ALCTQAGVIAPFKYHCMMKVQGLSPRVKLEL 330

BLAST of HG10000217 vs. NCBI nr
Match: KAA0043413.1 (APO protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 610.1 bits (1572), Expect = 1.1e-170
Identity = 293/329 (89.06%), Postives = 308/329 (93.62%), Query Frame = 0

Query: 1   MKTMAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMA+RR F +NVVQEFMLQRCYSSKV+LKKLRPMILKRIQDRAKNYPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 61  QQVLEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIR 120
           QQVLEAR  LIHGVSTLLKSFPVLSCK+CPEVYVG +GH IRSCGGYKRGAKNQVH+WIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNS 180
           GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NP+D++L+SST+NS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 AEGGGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 240
           AEGGGSGMDE P S  EMMLLATETIRAWETLRTGVQKLLMVYP KVCKYCSEVHVGPSG
Sbjct: 181 AEGGGSGMDE-PFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSG 240

Query: 241 HKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVL 300
           HKARLCGVFKYE+WRGSHFWEKADVDDLVPPKIVWHRR QDPPVLVD+GRD YGH PAV+
Sbjct: 241 HKARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVV 300

Query: 301 ALCIQAGALAPSKYHCMMKVQGLPPRSQL 330
           ALC QAGA+AP KYHCMMKVQGL PR  L
Sbjct: 301 ALCTQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of HG10000217 vs. NCBI nr
Match: XP_008455841.1 (PREDICTED: APO protein 4, mitochondrial [Cucumis melo] >XP_008455842.1 PREDICTED: APO protein 4, mitochondrial [Cucumis melo] >TYJ98297.1 APO protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 609.0 bits (1569), Expect = 2.4e-170
Identity = 292/329 (88.75%), Postives = 309/329 (93.92%), Query Frame = 0

Query: 1   MKTMAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMA+RR F +NVVQEFMLQRCYSSKV+LKKLRPMILKRIQDRAK+YPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKSYPIKGMTPVA 60

Query: 61  QQVLEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIR 120
           QQVLEAR  LIHGVSTLLKSFPVLSCK+CPEVYVG +GH IRSCGGYKRGAKNQVH+WIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNS 180
           GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NP+D++L+SST+NS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 AEGGGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 240
           AEGGGSGMDE P S  EMMLLATETIRAWETLRTGVQKLLMVYP KVCKYCSEVHVGPSG
Sbjct: 181 AEGGGSGMDE-PFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSG 240

Query: 241 HKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVL 300
           HKARLCGVFKYE+WRGSHFWEKADVDDLVPPKIVWHRR QDPPVLVD+GRD YGH PAV+
Sbjct: 241 HKARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVV 300

Query: 301 ALCIQAGALAPSKYHCMMKVQGLPPRSQL 330
           ALC+QAGA+AP KYHCMMKVQGL PR  L
Sbjct: 301 ALCMQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of HG10000217 vs. NCBI nr
Match: XP_023532078.1 (APO protein 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583.2 bits (1502), Expect = 1.4e-162
Identity = 280/328 (85.37%), Postives = 299/328 (91.16%), Query Frame = 0

Query: 4   MAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVAQQV 63
           MAFMALRR   ENVVQEFMLQRCYSSKVDLKKLRPMILKR+Q+RA NYPIKGM PVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 64  LEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIRGDL 123
           LEAR  LIHGVSTLLK+FPVLSCKFCPEVYVG+KGH IRSCGGYK GAKN+VHEWIRGDL
Sbjct: 61  LEARAKLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKHGAKNRVHEWIRGDL 120

Query: 124 NDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNS-AE 183
           NDI+VPVE+FHL HMFQDVIKHDERF+F+RVPAVVELCWQAGANPNDENLSSST NS  +
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFDRVPAVVELCWQAGANPNDENLSSSTWNSDGD 180

Query: 184 GGGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHK 243
           G G+G DE  LS  E+M+LA  TIRAWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGHK
Sbjct: 181 GNGNGRDEH-LSSHELMVLANTTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHK 240

Query: 244 ARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVLAL 303
           ARLCGVFKYE+WRGSHFWEKADVDDLVPPK VWHRR QDPPVLVDEGRD YGH PAVLAL
Sbjct: 241 ARLCGVFKYESWRGSHFWEKADVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLAL 300

Query: 304 CIQAGALAPSKYHCMMKVQGLPPRSQLK 331
           C+QAGA+APSKYHCMMKVQGL   SQLK
Sbjct: 301 CLQAGAIAPSKYHCMMKVQGLSHHSQLK 327

BLAST of HG10000217 vs. ExPASy Swiss-Prot
Match: Q9LSZ0 (APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2)

HSP 1 Score: 369.4 bits (947), Expect = 4.3e-101
Identity = 171/292 (58.56%), Postives = 222/292 (76.03%), Query Frame = 0

Query: 32  DLKKLRPMILKRIQDRAKNYPIKGMTPVAQQVLEARTTLIHGVSTLLKSFPVLSCKFCPE 91
           DL+KLRPMILKRI++RAK+YP+K + PVA+++L AR  LI  ++ LLK FPVL+CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 92  VYVGEKGHQIRSCGGYKRGAKNQVHEWIRGDLNDIIVPVEAFHLHHMFQDVIKHDERFNF 151
           V+VG++GH I +C  Y R   N++HEW+ G +NDI+VPVE++HLH++ Q VI+H ERF++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 152 ERVPAVVELCWQAGANPNDENLSSSTRNSAEGGGSGMDEQPLSGREMMLLATETIRAWET 211
           +RVPA++ELC QAGA   +E L  S  +      S  D + L   ++  +    + AWE 
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHD-NPQISEEDIRSLPAGDLKYVGANALMAWEK 213

Query: 212 LRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYENWRGSHFWEKADVDDLVPP 271
           +R GV+KLL+VYP+KVCK C EVHVGPSGHKARLCGVFKYE+WRG+H+WEKA V+DLVP 
Sbjct: 214 VRAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPE 273

Query: 272 KIVWHRRPQDPPVLVDEGRDCYGHTPAVLALCIQAGALAPSKYHCMMKVQGL 324
           K+VWHRRPQDP VLVDEGR  YGH PA+++LC   GA+ P KY C MK QGL
Sbjct: 274 KMVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGL 324

BLAST of HG10000217 vs. ExPASy Swiss-Prot
Match: Q8W4A5 (APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.1e-56
Identity = 122/331 (36.86%), Postives = 174/331 (52.57%), Query Frame = 0

Query: 31  VDLKKLRPMILKRIQDRAK--------NYPIKGMTPVAQQVLEARTTLIHGVSTLLKSFP 90
           VDL++     +K  +D+ K           +K + P+A +V  AR  LI+ +  L+K   
Sbjct: 100 VDLRRAARERVKNNKDKPKRPLPPPKNGMVVKSLVPLAYKVYNARIRLINNLHRLMKVVR 159

Query: 91  VLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIRGDLNDIIVPVEAFHLHHMFQDV 150
           V +C +C E++VG  GH  +SC G     +  +HEW    + D+IVP+EA+HL       
Sbjct: 160 VNACGWCNEIHVGPYGHPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGKR 219

Query: 151 IKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNSAEGGGS---GMDE--------- 210
           I+HDERF+  RVPAVVELC Q G    +       +     G S     DE         
Sbjct: 220 IRHDERFSIPRVPAVVELCIQGGVEIPEFPAKRRRKPIIRIGKSEFVDADETELPDPEPQ 279

Query: 211 ----------------QPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEV 270
                            P S  E + LA ET++AWE +R G +KL+ +Y  +VC YC EV
Sbjct: 280 PPPVPLLTELPVSEITPPSSEEETVSLAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPEV 339

Query: 271 HVGPSGHKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYG 325
           HVGP+GHKA+ CG FK++   G H W+ A +DDL+PP+ VWH    + P +  E R  YG
Sbjct: 340 HVGPTGHKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFYG 399

BLAST of HG10000217 vs. ExPASy Swiss-Prot
Match: Q9XIR4 (APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 122/329 (37.08%), Postives = 175/329 (53.19%), Query Frame = 0

Query: 34  KKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARTTLIHGVSTLLKSFPVLSCKFCPEV 93
           KKL  M +++  D  KN   +  + PVA QV++    LI G++ LL   PV +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 94  YVGEKGHQIRSCGGYKRGAKNQVHEWIRGDLNDIIVPVEAFHLHHMFQDVIKHDERFNFE 153
           +V   GH IR C G     +   H W++G +ND+++PVE++H++  F   IKH+ RF +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 154 RVPAVVELCWQAGA-----------------------------NPNDENLSSSTRNS-AE 213
           R+PA+VELC QAG                               P     SSS  +  AE
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 214 GGGSGMDEQ--PLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 273
               G+ E+  P +  ++  +A ET+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 274 HKARLCGVFKYENWR-GSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAV 329
           H  +LCG FK++ WR G H W+ A VD++ PP  VWH R      L    R  YG  PA+
Sbjct: 343 HSVKLCGEFKHQ-WRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPAL 402

BLAST of HG10000217 vs. ExPASy Swiss-Prot
Match: Q9FH50 (APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.0e-45
Identity = 111/334 (33.23%), Postives = 177/334 (52.99%), Query Frame = 0

Query: 22  MLQRCYSSKVDLKKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARTTLIHGVSTLLKS 81
           +++R    K  L+KL+P   + ++D   N   +  +  VA  V   R  L+ G+S ++  
Sbjct: 66  LIRRAKEEK-QLRKLQP--CRVLEDPPDNGLLVPELVDVAHCVHRCRNMLLSGLSKIIHH 125

Query: 82  FPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIRGDLNDIIVPVEAFHLH-HMF 141
            PV  C+ C EV++G++GH+IR+C G   G+++  H W RG ++D+++  + FHL+    
Sbjct: 126 VPVHRCRLCAEVHIGKQGHEIRTCTGPGSGSRSATHVWKRGRVSDVVLFPKCFHLYDRAV 185

Query: 142 QDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRN----SAEG----------GG 201
           +  + HDERF   ++ AV+ELC QAG +   E   S  R+    S EG          G 
Sbjct: 186 KPRVIHDERFTVPKISAVLELCIQAGVDL--EKFPSKRRSKPVYSIEGRIVDFEDVNDGN 245

Query: 202 SGM------------DEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSE 261
           S +            D      + +  L+ ET+ +W  +  GV+KL+  Y    C YC E
Sbjct: 246 SELAVTSTTTLIQEDDRCKEEKKSLKELSFETMESWFEMVLGVRKLMERYRVWTCGYCPE 305

Query: 262 VHVGPSGHKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWH-RRPQDPPVLVDEGRDC 321
           + VGP GHK R+C   K++   G H W++A +DD+V P  VWH R P D  VL +  +  
Sbjct: 306 IQVGPKGHKVRMCKATKHQMRDGMHAWQEATIDDVVGPTYVWHVRDPTDGSVLDNSLKRF 365

Query: 322 YGHTPAVLALCIQAGALAPSKYHCMMKVQGLPPR 327
           YG  PAV+ +C+Q GA  P +Y+ MM++  + P+
Sbjct: 366 YGKAPAVIEMCVQGGAPVPDQYNSMMRLDVVYPQ 394

BLAST of HG10000217 vs. ExPASy TrEMBL
Match: A0A0A0LQG5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 6.3e-172
Identity = 295/331 (89.12%), Postives = 311/331 (93.96%), Query Frame = 0

Query: 1   MKTMAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMA+RR F +NVVQEFMLQRCYSSKV+LKKLRPMILKRIQDRAKNYPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 61  QQVLEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIR 120
           QQVLEAR  LIHGVSTLLKSFPVLSCKFCPEVYVGE+GH IRSCGGYKRGAKNQVH+WIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNS 180
           GDL DIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAGANP+D+NL+SST+NS
Sbjct: 121 GDLKDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNS 180

Query: 181 AEGGGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 240
           AEGGGSGMDE PLS  EMMLLATETIRAWETLRTGVQKLLMVYP KVCKYCSEVHVGPSG
Sbjct: 181 AEGGGSGMDE-PLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSG 240

Query: 241 HKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVL 300
           HKARLCGVF YE+WRGSHFWEKADVDDLVPPKIVWHRR QDPPVLVD+G+D YGH PAV+
Sbjct: 241 HKARLCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVV 300

Query: 301 ALCIQAGALAPSKYHCMMKVQGLPPRSQLKL 332
           ALC QAG +AP KYHCMMKVQGL PR +L+L
Sbjct: 301 ALCTQAGVIAPFKYHCMMKVQGLSPRVKLEL 330

BLAST of HG10000217 vs. ExPASy TrEMBL
Match: A0A5A7TJ18 (APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180 PE=4 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.3e-171
Identity = 293/329 (89.06%), Postives = 308/329 (93.62%), Query Frame = 0

Query: 1   MKTMAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMA+RR F +NVVQEFMLQRCYSSKV+LKKLRPMILKRIQDRAKNYPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVA 60

Query: 61  QQVLEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIR 120
           QQVLEAR  LIHGVSTLLKSFPVLSCK+CPEVYVG +GH IRSCGGYKRGAKNQVH+WIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNS 180
           GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NP+D++L+SST+NS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 AEGGGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 240
           AEGGGSGMDE P S  EMMLLATETIRAWETLRTGVQKLLMVYP KVCKYCSEVHVGPSG
Sbjct: 181 AEGGGSGMDE-PFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSG 240

Query: 241 HKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVL 300
           HKARLCGVFKYE+WRGSHFWEKADVDDLVPPKIVWHRR QDPPVLVD+GRD YGH PAV+
Sbjct: 241 HKARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVV 300

Query: 301 ALCIQAGALAPSKYHCMMKVQGLPPRSQL 330
           ALC QAGA+AP KYHCMMKVQGL PR  L
Sbjct: 301 ALCTQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of HG10000217 vs. ExPASy TrEMBL
Match: A0A1S3C343 (APO protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495942 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.2e-170
Identity = 292/329 (88.75%), Postives = 309/329 (93.92%), Query Frame = 0

Query: 1   MKTMAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMA+RR F +NVVQEFMLQRCYSSKV+LKKLRPMILKRIQDRAK+YPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKSYPIKGMTPVA 60

Query: 61  QQVLEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIR 120
           QQVLEAR  LIHGVSTLLKSFPVLSCK+CPEVYVG +GH IRSCGGYKRGAKNQVH+WIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNS 180
           GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NP+D++L+SST+NS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 AEGGGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 240
           AEGGGSGMDE P S  EMMLLATETIRAWETLRTGVQKLLMVYP KVCKYCSEVHVGPSG
Sbjct: 181 AEGGGSGMDE-PFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSG 240

Query: 241 HKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVL 300
           HKARLCGVFKYE+WRGSHFWEKADVDDLVPPKIVWHRR QDPPVLVD+GRD YGH PAV+
Sbjct: 241 HKARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVV 300

Query: 301 ALCIQAGALAPSKYHCMMKVQGLPPRSQL 330
           ALC+QAGA+AP KYHCMMKVQGL PR  L
Sbjct: 301 ALCMQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of HG10000217 vs. ExPASy TrEMBL
Match: A0A5D3BGQ1 (APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00100 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.2e-170
Identity = 292/329 (88.75%), Postives = 309/329 (93.92%), Query Frame = 0

Query: 1   MKTMAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVA 60
           MKTMAFMA+RR F +NVVQEFMLQRCYSSKV+LKKLRPMILKRIQDRAK+YPIKGMTPVA
Sbjct: 1   MKTMAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKSYPIKGMTPVA 60

Query: 61  QQVLEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIR 120
           QQVLEAR  LIHGVSTLLKSFPVLSCK+CPEVYVG +GH IRSCGGYKRGAKNQVH+WIR
Sbjct: 61  QQVLEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIR 120

Query: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNS 180
           GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELC QAG NP+D++L+SST+NS
Sbjct: 121 GDLNDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNS 180

Query: 181 AEGGGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 240
           AEGGGSGMDE P S  EMMLLATETIRAWETLRTGVQKLLMVYP KVCKYCSEVHVGPSG
Sbjct: 181 AEGGGSGMDE-PFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSG 240

Query: 241 HKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVL 300
           HKARLCGVFKYE+WRGSHFWEKADVDDLVPPKIVWHRR QDPPVLVD+GRD YGH PAV+
Sbjct: 241 HKARLCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVV 300

Query: 301 ALCIQAGALAPSKYHCMMKVQGLPPRSQL 330
           ALC+QAGA+AP KYHCMMKVQGL PR  L
Sbjct: 301 ALCMQAGAIAPFKYHCMMKVQGLSPRVNL 328

BLAST of HG10000217 vs. ExPASy TrEMBL
Match: A0A6J1I7G7 (APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE=4 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.0e-162
Identity = 281/327 (85.93%), Postives = 296/327 (90.52%), Query Frame = 0

Query: 4   MAFMALRRNFSENVVQEFMLQRCYSSKVDLKKLRPMILKRIQDRAKNYPIKGMTPVAQQV 63
           MAFMALRR   ENVVQEFMLQRCYSSKVDLKKLRPMILKR+Q+RA NYPIKGM PVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 64  LEARTTLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIRGDL 123
           LEAR  LIHGVSTLLK+FPVLSCKFCPEVYVG+KGH IRSCGGYKRGAKN+VHEWIRGDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKRGAKNRVHEWIRGDL 120

Query: 124 NDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNSAEG 183
           NDI+VPVE+FHL HMFQDVIKHDERF+FERVPAVVELCWQAGANPNDENLSSST NS   
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDENLSSSTWNS--- 180

Query: 184 GGSGMDEQPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKA 243
            G G DE  LS  E+M+LA  TIRAWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGHKA
Sbjct: 181 DGDGRDEH-LSSHELMVLANSTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKA 240

Query: 244 RLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAVLALC 303
           RLCGVFKYE+WRGSHFWEK DVDDLVPPK VWHRR QDPPVLVDEGRD YGH PAVLALC
Sbjct: 241 RLCGVFKYESWRGSHFWEKGDVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALC 300

Query: 304 IQAGALAPSKYHCMMKVQGLPPRSQLK 331
           IQAGA+APSKYHCMMKVQGL   SQLK
Sbjct: 301 IQAGAIAPSKYHCMMKVQGLSHHSQLK 323

BLAST of HG10000217 vs. TAIR 10
Match: AT3G21740.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 369.4 bits (947), Expect = 3.0e-102
Identity = 171/292 (58.56%), Postives = 222/292 (76.03%), Query Frame = 0

Query: 32  DLKKLRPMILKRIQDRAKNYPIKGMTPVAQQVLEARTTLIHGVSTLLKSFPVLSCKFCPE 91
           DL+KLRPMILKRI++RAK+YP+K + PVA+++L AR  LI  ++ LLK FPVL+CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 92  VYVGEKGHQIRSCGGYKRGAKNQVHEWIRGDLNDIIVPVEAFHLHHMFQDVIKHDERFNF 151
           V+VG++GH I +C  Y R   N++HEW+ G +NDI+VPVE++HLH++ Q VI+H ERF++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 152 ERVPAVVELCWQAGANPNDENLSSSTRNSAEGGGSGMDEQPLSGREMMLLATETIRAWET 211
           +RVPA++ELC QAGA   +E L  S  +      S  D + L   ++  +    + AWE 
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHD-NPQISEEDIRSLPAGDLKYVGANALMAWEK 213

Query: 212 LRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYENWRGSHFWEKADVDDLVPP 271
           +R GV+KLL+VYP+KVCK C EVHVGPSGHKARLCGVFKYE+WRG+H+WEKA V+DLVP 
Sbjct: 214 VRAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPE 273

Query: 272 KIVWHRRPQDPPVLVDEGRDCYGHTPAVLALCIQAGALAPSKYHCMMKVQGL 324
           K+VWHRRPQDP VLVDEGR  YGH PA+++LC   GA+ P KY C MK QGL
Sbjct: 274 KMVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGL 324

BLAST of HG10000217 vs. TAIR 10
Match: AT5G57930.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 221.9 bits (564), Expect = 7.8e-58
Identity = 122/331 (36.86%), Postives = 174/331 (52.57%), Query Frame = 0

Query: 31  VDLKKLRPMILKRIQDRAK--------NYPIKGMTPVAQQVLEARTTLIHGVSTLLKSFP 90
           VDL++     +K  +D+ K           +K + P+A +V  AR  LI+ +  L+K   
Sbjct: 100 VDLRRAARERVKNNKDKPKRPLPPPKNGMVVKSLVPLAYKVYNARIRLINNLHRLMKVVR 159

Query: 91  VLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIRGDLNDIIVPVEAFHLHHMFQDV 150
           V +C +C E++VG  GH  +SC G     +  +HEW    + D+IVP+EA+HL       
Sbjct: 160 VNACGWCNEIHVGPYGHPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGKR 219

Query: 151 IKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNSAEGGGS---GMDE--------- 210
           I+HDERF+  RVPAVVELC Q G    +       +     G S     DE         
Sbjct: 220 IRHDERFSIPRVPAVVELCIQGGVEIPEFPAKRRRKPIIRIGKSEFVDADETELPDPEPQ 279

Query: 211 ----------------QPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEV 270
                            P S  E + LA ET++AWE +R G +KL+ +Y  +VC YC EV
Sbjct: 280 PPPVPLLTELPVSEITPPSSEEETVSLAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPEV 339

Query: 271 HVGPSGHKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYG 325
           HVGP+GHKA+ CG FK++   G H W+ A +DDL+PP+ VWH    + P +  E R  YG
Sbjct: 340 HVGPTGHKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFYG 399

BLAST of HG10000217 vs. TAIR 10
Match: AT5G57930.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 221.9 bits (564), Expect = 7.8e-58
Identity = 122/331 (36.86%), Postives = 174/331 (52.57%), Query Frame = 0

Query: 31  VDLKKLRPMILKRIQDRAK--------NYPIKGMTPVAQQVLEARTTLIHGVSTLLKSFP 90
           VDL++     +K  +D+ K           +K + P+A +V  AR  LI+ +  L+K   
Sbjct: 103 VDLRRAARERVKNNKDKPKRPLPPPKNGMVVKSLVPLAYKVYNARIRLINNLHRLMKVVR 162

Query: 91  VLSCKFCPEVYVGEKGHQIRSCGGYKRGAKNQVHEWIRGDLNDIIVPVEAFHLHHMFQDV 150
           V +C +C E++VG  GH  +SC G     +  +HEW    + D+IVP+EA+HL       
Sbjct: 163 VNACGWCNEIHVGPYGHPFKSCKGPNTSQRKGLHEWTNSVIEDVIVPLEAYHLFDRLGKR 222

Query: 151 IKHDERFNFERVPAVVELCWQAGANPNDENLSSSTRNSAEGGGS---GMDE--------- 210
           I+HDERF+  RVPAVVELC Q G    +       +     G S     DE         
Sbjct: 223 IRHDERFSIPRVPAVVELCIQGGVEIPEFPAKRRRKPIIRIGKSEFVDADETELPDPEPQ 282

Query: 211 ----------------QPLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEV 270
                            P S  E + LA ET++AWE +R G +KL+ +Y  +VC YC EV
Sbjct: 283 PPPVPLLTELPVSEITPPSSEEETVSLAEETLQAWEEMRAGAKKLMRMYRVRVCGYCPEV 342

Query: 271 HVGPSGHKARLCGVFKYENWRGSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYG 325
           HVGP+GHKA+ CG FK++   G H W+ A +DDL+PP+ VWH    + P +  E R  YG
Sbjct: 343 HVGPTGHKAQNCGAFKHQQRNGQHGWQSAVLDDLIPPRYVWHVPDVNGPPMQRELRSFYG 402

BLAST of HG10000217 vs. TAIR 10
Match: AT1G64810.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 214.5 bits (545), Expect = 1.3e-55
Identity = 122/329 (37.08%), Postives = 175/329 (53.19%), Query Frame = 0

Query: 34  KKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARTTLIHGVSTLLKSFPVLSCKFCPEV 93
           KKL  M +++  D  KN   +  + PVA QV++    LI G++ LL   PV +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 94  YVGEKGHQIRSCGGYKRGAKNQVHEWIRGDLNDIIVPVEAFHLHHMFQDVIKHDERFNFE 153
           +V   GH IR C G     +   H W++G +ND+++PVE++H++  F   IKH+ RF +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 154 RVPAVVELCWQAGA-----------------------------NPNDENLSSSTRNS-AE 213
           R+PA+VELC QAG                               P     SSS  +  AE
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 214 GGGSGMDEQ--PLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 273
               G+ E+  P +  ++  +A ET+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 274 HKARLCGVFKYENWR-GSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAV 329
           H  +LCG FK++ WR G H W+ A VD++ PP  VWH R      L    R  YG  PA+
Sbjct: 343 HSVKLCGEFKHQ-WRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPAL 402

BLAST of HG10000217 vs. TAIR 10
Match: AT1G64810.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 214.5 bits (545), Expect = 1.3e-55
Identity = 122/329 (37.08%), Postives = 175/329 (53.19%), Query Frame = 0

Query: 34  KKLRPMILKRIQDRAKN-YPIKGMTPVAQQVLEARTTLIHGVSTLLKSFPVLSCKFCPEV 93
           KKL  M +++  D  KN   +  + PVA QV++    LI G++ LL   PV +C  C  V
Sbjct: 127 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 186

Query: 94  YVGEKGHQIRSCGGYKRGAKNQVHEWIRGDLNDIIVPVEAFHLHHMFQDVIKHDERFNFE 153
           +V   GH IR C G     +   H W++G +ND+++PVE++H++  F   IKH+ RF +E
Sbjct: 187 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 246

Query: 154 RVPAVVELCWQAGA-----------------------------NPNDENLSSSTRNS-AE 213
           R+PA+VELC QAG                               P     SSS  +  AE
Sbjct: 247 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 306

Query: 214 GGGSGMDEQ--PLSGREMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 273
               G+ E+  P +  ++  +A ET+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 307 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 366

Query: 274 HKARLCGVFKYENWR-GSHFWEKADVDDLVPPKIVWHRRPQDPPVLVDEGRDCYGHTPAV 329
           H  +LCG FK++ WR G H W+ A VD++ PP  VWH R      L    R  YG  PA+
Sbjct: 367 HSVKLCGEFKHQ-WRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPAL 426

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901944.14.2e-17891.84APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, ... [more]
XP_004151879.11.3e-17189.12APO protein 4, mitochondrial [Cucumis sativus] >XP_031737269.1 APO protein 4, mi... [more]
KAA0043413.11.1e-17089.06APO protein 4 [Cucumis melo var. makuwa][more]
XP_008455841.12.4e-17088.75PREDICTED: APO protein 4, mitochondrial [Cucumis melo] >XP_008455842.1 PREDICTED... [more]
XP_023532078.11.4e-16285.37APO protein 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LSZ04.3e-10158.56APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2[more]
Q8W4A51.1e-5636.86APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1[more]
Q9XIR41.8e-5437.08APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1[more]
Q9FH502.0e-4533.23APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LQG56.3e-17289.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1[more]
A0A5A7TJ185.3e-17189.06APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180... [more]
A0A1S3C3431.2e-17088.75APO protein 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495942 PE=4 SV=1[more]
A0A5D3BGQ11.2e-17088.75APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00100 ... [more]
A0A6J1I7G72.0e-16285.93APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE... [more]
Match NameE-valueIdentityDescription
AT3G21740.13.0e-10258.56Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT5G57930.17.8e-5836.86Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT5G57930.27.8e-5836.86Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT1G64810.11.3e-5537.08Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT1G64810.21.3e-5537.08Arabidopsis thaliana protein of unknown function (DUF794) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023342APO domainPFAMPF05634APO_RNA-bindcoord: 197..308
e-value: 1.3E-20
score: 73.6
coord: 29..167
e-value: 4.1E-41
score: 140.6
IPR023342APO domainPROSITEPS51499APOcoord: 85..170
score: 24.200674
IPR023342APO domainPROSITEPS51499APOcoord: 227..312
score: 21.863625
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..184
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..193
NoneNo IPR availablePANTHERPTHR10388:SF20APO PROTEIN 4, MITOCHONDRIALcoord: 12..326
NoneNo IPR availablePANTHERPTHR10388EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1coord: 12..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000217.1HG10000217.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding