Cucsat.G8166 (gene) Cucumber (B10) v3

Overview
NameCucsat.G8166
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionProtein of unknown function (DUF630 and DUF632)
Locationctg1557: 1904377 .. 1910225 (+)
RNA-Seq ExpressionCucsat.G8166
SyntenyCucsat.G8166
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATTTAGAAAAAATGGGAAACCATTTAAAGAAACTATACAGTTAAACAGAATATATGTTGGGTTTGAAAGTTGAAGCAGAATCAAAGGAAATTTGGAGCAATCTTCATGTAAAAGACACAGAGAAAGAAAGAAACCGAACACCATTGATGATGATGATGGGTTATGAATAATAATAGCAATTGGTAGGTGCTAAGCTAAGCTAAAAAAGCTAAACCAAGCCAATTCATTGGATGCTTCCCATTTCTCGATTTTCGAGCTTCCCAACTCTCTCTCTCTCTCTTCTTTCTCATTTTTCATTCTGTAATCTGTAAAAAGGACGAACACATAAAGGCGACGGACACTCACAACCTTCTTTTTCGACGTTACTCTAAATTTCCCTTTCCGTTTCACAATTTTTTCTTCTTCTCATAAACCACCTATGGTTGTTGTCGCATTATCACTTGCCTTCTCTTGTTCTTCTTCTTTGGGCAGTGGATTCTGCAGCTGACACCCTTCTACTTGTCAGACCCACTGTTCTCATGTTCTTTTTCCACCGTTTCATCAATTAAAAATAGCTTCTACAAGATTTTTCTGAGTTGGGTCTTGGACGTTTTTTTTAAAAAAAACTAATTATTACATTTGTATTTTTTACTGCTTTGTTTGTTTGGTGGTGAGAGTAATGGGGTTTTGAAGCTGTGTGATTCTGGTTGTTTATTCTTCTTATTTTGTCTTTTTTGCGAATCCTTGAGCCAATCAAGCTGCTGTGAATCGTAATGCTCGGGCTTTAGCATAGAAGATTCGAGTAGAAGAATAAGAGGAGTTGAAGTTTGAACCCCAGCTGAGAAAAGCATTGTCCGGTTATTTTGGTATCTCTTTATCTTTTCATGGATCTTGTAATTTGGTCACTTGGATATTGTTGGTTGTGAAATTTTTGTAATGAACATTGGATTTTAAGCCATTTGTTTGGGTTTTCGAATCACTTAAACTTGGAAGAGGGAGATTCTGTGGAGAAGTTGAGTTTATTCAAACATTGTCAAAGGTAAGCCCAACAGGAATTTGTTATTAGGGAGTTTTTAGCTTTGGGGTTTAGTTGTATCAGCGTTTCTCCTTAATGATTGTTCTGCTTGAGTTTCTTTCAATTGGGTCTACGATTTCTGCTGCGTTTGTGATTGAAATTCTATAGCAGACATCTTTTATTTGGTATCCATTTGGTGAAATGGGTTGTGCTGCCTCAAGTATTGATGAGGAGGAGAGAGTGAAGGCTTGTAGGGAGAGGAAGAAGCTAATGAAACAGTTAATAGGGTTTAGGAAAGAATTTGCAGATTCCTTGCTAGCTTACTTGAGGGCATTGAAAAATACAGGTGCAACCCTTAGACAATTTACTGAGTCTGAAACATTGGAGCTTGAAGATACTATTTATGGCTTGGCATCTCCTCCATCACCACCTCCCCCTTTGCCTCCCTCTCCACCCCCACCTCCACCTTTTAGCCCTGATTTGAGGAAGCATGGTGCTGAAGATGCACAAAAAGATGAGCTTGCCCAAGAAGAAAGCGTAGTTATCGACGAGGATGAGGATGAGGATGATCACACTCCATCACCTCCAATCTTGAGCTCATCTTGGGAGTATTGGGATCCTTTTGAGCATTCTGCTGTGCATCAACAAAAAAAGAGTGAAACTGTGGGGCCAGTTGAAGAGGAAAATTGGGCAGAGACTAGAAGCGAATTTGAGGAAGAGGATAAGAAGGAGGAAGCTGTGGAAGATGTCGTGAATCCAGTACCTACGACTCTAGAGCAAAGGGAATTGATAAGTTGTATTTCTTCTACGAGTAGCTTGCATATGAAGGTGGCTACAGACATGGGCATGATATCATGGAAAAATAAGAAAACCTTGGGAGCTGTGGTCAAGGAACTCGATGAGTATTTTCTTAAAGCATCGGGCGGCATAAAAGAAATAGCAGTTCTCATTGATGTCACTGTTGGAAACGATTTTCCACCCCATAATTTCAGGGAAAACAAGAGTAAGTATTCTCTTTTTTTAATCATTTCTTTGTCTCTTTTGTGCAGTTGATCTCGTTTTTAACTTTCATGATTTTCCTTGAAACTGATGAAAAATTAACACTTCCTTTCTCCAAAAATTAAAAAAAAAATGGTATGGTTTCATTTTTTCCCTATGATTGTGGCATCGAACTTATTTAAAGTAGTTAATATTGAGCATACTGCTAATGCATCCCTCTTGCAATTGTAGTCTTTGCCTTCCAATGTCTTTGCAATTAACCTTGAAAGCCTTCAGCACTCCTCGATTTTCTTTTATGCTGATGTTTTTGTTTTAGTCTCTTTAGTGTTTGTTATGTGCTGATATTATTTGTTAGTTTGTAGTCGAGGTCTCAGTCAACTTCTTTTGCCTTCTTTAGGGCTGGCATTTTTCACAGGATGTTACGTTCTTCTTTCTTGTTTTTAAGATGCCTACTCGGAGATCTTGGAAAATAAGTGTATAACCAATAAGATTCAGGCTTTTGTTTAACCGTAGTTTCAGATAATACAATGCTTCCTTTACTAATTTAGTCTCTAAACTTAAAAAGTATCTAAGAAGTCTAAAATTTCAATGTCATGCCTAATAGTTTTTTTTTTATTTCAGTGAGTTTTTCGTTTTTAATGGATTTTTTACCTTCGGAAAATGACTCACCGATCATGAACCTCGACACAAGAGTTTATTAGATATTTTAAATTTCCAGAGCTATTAGACAGAATACTGAAAGTTTAAACGCCTTTGAAATGCTTTTTTAAATTCAAAAGTGTATTAGACACAATATTAAAAGTTTATAACCTCTTAGATGCTCTTCTAATTTCAGAGATATTTAGACATTAACTCTATACCTTTGTTTCTTGATTAAAAAAGGGAAAGTATCATAGTTTCTTTCATAAGCTCTATTACTTTAATGCACCATCAGGCCTGCTTCAGTTTCCATTACTTTCCCGTAAATTCCTCAAGCAACTATTGATCCTATGATTACAGGGAAGAGAAGCAATTCTGCTAAAGTGTTCAATGCATTATCGCGGAGGTGGTCATCTAGCACACTTCAGTTTGCCACAGATGCAGCTGAGTTTCTTGGTCCCAATGAACCGTGCCGACCTGGAGCTCATTGTATCACCCTAAAAAAGCTTTATGCAGCAGAACAAAGGCTTCAAAAGGACATTAAGGTAAAAAGAAAAAAAATACAAGAACCGTGCTGATATAAACTGTTGTGCAAAATCTCAGAATATATGTTTCGTTTGTCTCATCATTGCCATAATACTCTTAGGCTATGCTGACCTATGTCCCACAGAAATAGATGATAGCAAAAATGTGAAATATTGTAAAAAACTCGTACGATCATCTTGTAGTTGTTGATATTGTTGGCGATATATAATTAAATTTACCTTCAACCACCCATTTAAGCTTTTGGTTGAATTGACGATTCAATAGATATGAATTCCATCTTTAGGATTAATATCTGCTTCAAGAATCAATGGAACTCTTGAGCGTTTAATGGAATTTGATCATGATGAAGAAATTCCTCATCCATATCTTAATTCAGGACAGCTTAATTTGAATCTTTTACCCTTCTTCTCCCAACACCTACTTGCTCTTGCCATGTGGAAAGTAACTGCGCAAAGAATTTAAGATTTTGCTCGGTGCCTGATTGTTGGGGGTGTTAATATGCAAGGCGAGGTCCACCCAAAGACATCCTGATATGCAAGATGTGGTTGACCAATGACATTCTAATATTCTGTTACACTCATCTCCACGATGACAATCAAATTTTTTTCCTTTGATCTTACCTGAGAACATTTTCGTGATTTGTCCATTATATCTTAAAGTTGTGGACCAAGTTCCAAGAGGGTGTTTGACCCCTAACTCAAGTGGGTGGAGTGGGCTATTATAGTTGATTTTCATGTTTGGGATTTCAATTATAATAGTTGGTGTTCTAACTATTAGAATATAACCAACAATTAACTGTACTATTGCTATTTTTTTTATAAGAATCTGTACTATTAATATTTTTAATCCCTCTTTGTTCATGTTTACTCTTTTCTACTCATTCTCTTGTTGTTACAGTCTTTACTATTTCTTACCTAGACCAAATAGTTTGCACTCTAAATATAGACTTTTATAACTCATATTAAAATAGTTTTCATCCCAAAACACGGACTATTATAATTCATAGGCGACAATAACCACTAACTTAATCAACTTAGCGTCTCAAACAACTCCTTAGAGGTTGAGTTCACAGAACCGGTACCTTGTTTTGCTAGGAAGTCTTGTAGATAGCTGGAGCAACTGTTTTCATTCATCAGTAAACTTTAATTGAACTGGAACCTACTGGCTACTGTAACATCATGCATGGACTTTGTTTAATTGTTCTTTGCCTTGTAGATTATTTTTGAAGACTGTAACTTACGGTAGTATGAAATGATGCCAATTCAAGGCAGGTTTGATTGATATATCAATACTGGTAGTTGTTATACATCCACTGTTGAATTTCCTATATATCTTTTGTAATGTTGATTGTGTATTATGTATGTATGTGTATGTGTGTATTTTGTTTTTGTGATTATTTTTTCTTATTGTTTGATGGCCTTTGTTAAGAAATAAGAGGACCTTGTATTTGATTCAGAAAATAGAAGAAATACAGTAATTTTGTTTCTTCTCAAATTCAAGTACTACTTGCATATGATTCTATATCCTTCATAAGTTTTATTGAGATTCTTTTGTCCTTGTTTTTATCAAATTTTCATATTTTGTAGGAAGAAGAAGGTACCAATCTAGAGTATAAAAAGAAAGTTTTGCTACTACAAAAGCAAGAGGATGAGCATCATGATTGGACCAAAATTGAGAAAACTCGCCAGACTGTTGAGGGTTTGGAGTCTGACATAATACGTCTGCGGCAAGCTATAGGTGAACATTGTGCTTCTATATTGGCGCTAATGGATGAGGAACTGTATCCACAATTGGTTGCTTTAACTTCGGGGTAAATGCACAAAGGTTTTATTTGAGTCCTGGCGATAAAAGTTTACCTTCCTTTATATGCTTATTACAGGAAAATTCAACAGTGCCACTTTCTGAATGAATATGAACGAAGACCGATTTCTTGTTCTTCTCTGTTATCCTGTGATTCTTTTTCCCCCGTACTATCTGATATCGGTGACTTGGCTGCCTTCCTTTATTCTCCCCACTAAATGATTTCATCCTCATTCATTCTGCCCGTTAAGGAAGTTGGCGACTTTTTAGACTGCATATCTTTCGTGAGCCAAACCTGCCTAATAACAATTACACAAATTTTGCAAAAGCTATACTGAGGCTAAAATAATCTCTCTACCTTCCGCCTTTTGGCAAAGATCTCTGTGTTCTCATGTAAATTGAGTCTTATAATTATCTGTCATCTGTCTTTTTTAATTTTTCTGTTTCATTTTTGTTCAGGTTATTGCACATGTGGAAGATAATGTCTGAATGCCATCAAGTTCAGAATCAAATCTCCCAGCAGTTGAACCATCATATCAACAACCATGACATAGATCTAAGTACAGATTACCATCGCCGGGCCACAGCTCAGCTTGCAGCTGAGATAACAGTCTGGTACAGCAGCTTCTGCAATCTCGTAAAATATCAGCGAGAGTATGTAAAAACCCTCTGTAGGTGGACTCAACTTACTGACTTCCTTGTCGATCATGATAGACAGAGTGTTTGTGCATCTGTGGTTCTTAACATCTGTGGAAAATGGCAGGATACACTTGAAAGATTACCTGACAAGGTACTTATCTTGGTGCATTTTTGTGAATATTTTGGCCTTGATCTGATGATTAAAGGTATGGCTTAACGGAGAATGTTGTCATGGTAAATGTTGATCTTTTAGTTCAAAGTTGGTTCAAAATTAAAAAATAAAAAAAGATCAAAATTCAATCCTAAATTAAGCTTATCGTTTTAGAATATCACATCACACTTGGTTAGGAAATTATAAACCGCATTATCTTAGAAGGCGTAGATATGCTTTAAATGCTAAACCATGCTCATTTTGGCTCTAATTGAGCATATTTGAAATTTATCTTTGATAGAAGATAAGATGCTTCTTTGACAAATCATACTTTATATTATTCTGTATGTCTAATCATATGCTGAAAGATTGTAATCTTCTTTTATCCTCCTTTAAGTATCAATTATTTATTAACTTATTTTATTATGTCTTTTGGTTGGTTCTCCTTTGTTGTGGCTTCCATTTTTGTGTAATTACTCGCTTGTTTGCACTATTGCCATTAGACAGATGTAATTGGTAGATTTCTACTCACCAAGTGAATGTTTTGCCTCTGCCACCATTAATTACTTAAAGGACTTTAAAAATTGGCCTACTTTAAGTATATTTTATGAATTTTTCCCATTTACTTTGCACTAAAACCATCAAACTTCTATTCCCTTCCTTTCTTTCTACTGTGTTCTTCACTCGATTCTCTCCCTGTCTTTCTTTCAGGCAGCTTCAGAGGCCATCAAGAATCTCTTGTCAGCCATAAACTCTCTACTACTCCAGCAAGTGGAGGAACAAAACCTCCAAAGGAAATACGAAAAGCTGGATAAGAGACTTCAAAAAGAGATGCACTCATTGGCGGAGATGGAAAAGAAACTTGGGGGAAACTCTCTGTCTGAAGTTGGAAATGACAATTTGAACTCCAAGAATCCTCTTATACTAAAGCGTGCAAAAACCGATGCCTTGAAGAAACTTGTTGATACCGAGAAGGCCAAATATCTAAACTCCATCCAGGTTAGCCGAGCCATGACTTCAAACCATCTGAAAACAGGCCTCCCCAATGTCTTTCAGGCACTTATGGGATTTGCTAGTTTATCTGTAAAGGGTATGGAGTCTGTTTGTAGTAATGTTACACCACCCCAGGAATGTTGTGATGATGCAACAGTGAGCTCTACTAATTGAGTTTATTAGCCATTTTGGGATAGTAATGGGCCCATAGAACATGTTATTTTTCTTTTTTATCAACAGAAGTTTACTTGCTCTGCAGAAAATTTCCTATATTTTATTGGATGTCAGTCTAATCACTATACCAATACACGAAGTCCAGATTTCTATCAAGTAATGAAATAATAATATTCTGCTGATTAGGTCTATTGAGTCCTTGGTTT

Coding sequence (CDS)

ATGGGTTGTGCTGCCTCAAGTATTGATGAGGAGGAGAGAGTGAAGGCTTGTAGGGAGAGGAAGAAGCTAATGAAACAGTTAATAGGGTTTAGGAAAGAATTTGCAGATTCCTTGCTAGCTTACTTGAGGGCATTGAAAAATACAGGTGCAACCCTTAGACAATTTACTGAGTCTGAAACATTGGAGCTTGAAGATACTATTTATGGCTTGGCATCTCCTCCATCACCACCTCCCCCTTTGCCTCCCTCTCCACCCCCACCTCCACCTTTTAGCCCTGATTTGAGGAAGCATGGTGCTGAAGATGCACAAAAAGATGAGCTTGCCCAAGAAGAAAGCGTAGTTATCGACGAGGATGAGGATGAGGATGATCACACTCCATCACCTCCAATCTTGAGCTCATCTTGGGAGTATTGGGATCCTTTTGAGCATTCTGCTGTGCATCAACAAAAAAAGAGTGAAACTGTGGGGCCAGTTGAAGAGGAAAATTGGGCAGAGACTAGAAGCGAATTTGAGGAAGAGGATAAGAAGGAGGAAGCTGTGGAAGATGTCGTGAATCCAGTACCTACGACTCTAGAGCAAAGGGAATTGATAAGTTGTATTTCTTCTACGAGTAGCTTGCATATGAAGGTGGCTACAGACATGGGCATGATATCATGGAAAAATAAGAAAACCTTGGGAGCTGTGGTCAAGGAACTCGATGAGTATTTTCTTAAAGCATCGGGCGGCATAAAAGAAATAGCAGTTCTCATTGATGTCACTGTTGGAAACGATTTTCCACCCCATAATTTCAGGGAAAACAAGAGGAAGAGAAGCAATTCTGCTAAAGTGTTCAATGCATTATCGCGGAGGTGGTCATCTAGCACACTTCAGTTTGCCACAGATGCAGCTGAGTTTCTTGGTCCCAATGAACCGTGCCGACCTGGAGCTCATTGTATCACCCTAAAAAAGCTTTATGCAGCAGAACAAAGGCTTCAAAAGGACATTAAGGAAGAAGAAGGTACCAATCTAGAGTATAAAAAGAAAGTTTTGCTACTACAAAAGCAAGAGGATGAGCATCATGATTGGACCAAAATTGAGAAAACTCGCCAGACTGTTGAGGGTTTGGAGTCTGACATAATACGTCTGCGGCAAGCTATAGGTGAACATTGTGCTTCTATATTGGCGCTAATGGATGAGGAACTGTATCCACAATTGGTTGCTTTAACTTCGGGGTAA

Protein sequence

MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESETLELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDEDEDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAVEDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKASGGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLGPNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEKTRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG
Homology
BLAST of Cucsat.G8166 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.0e-32
Identity = 127/412 (30.83%), Postives = 198/412 (48.06%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGC  S ID +E V  C+ RK+ +K L+  R+  + S   YLR+L+  G++L  F+  ET
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSP-PPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDE 120
                 ++   +PPSP PP PP P PPPPP SP     G+E            +      
Sbjct: 61  -----PLHLHHNPPSPSPPPPPPPRPPPPPLSP-----GSETTTWTTTTTSSVL-----P 120

Query: 121 DEDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAE---TRSEFEEEDKK 180
                 P PP  SS+W++WDPF    +     S       EE W E   T +        
Sbjct: 121 PPPPPPPPPPPPSSTWDFWDPF----IPPPPSS------SEEEWEEETTTATRTATGTGS 180

Query: 181 EEAVEDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYF 240
           + AV     P   T +   ++S  S  +       +++ ++  +N K L  ++KE+DEYF
Sbjct: 181 DAAV--TTAPTTATPQASSVVSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEVDEYF 240

Query: 241 LKASGGIKEIAVLIDVTVG-NDFPPHNFRENKRKRSNSAKVFNALS---RRWSSSTLQFA 300
           LKA+     ++ L++++    DF  H+        SN     N  S   R ++ S L   
Sbjct: 241 LKAADSGAPLSSLLEISTSITDFSGHSKSGKMYSSSNYECNLNPTSFWTRGFAPSKLSEY 300

Query: 301 TDAAEFLGPNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEH 360
            +A   +G N  C  G+H  T+ +LYA E++L +++K  E   ++++KKV  +++ E + 
Sbjct: 301 RNAGGVIGGN--CIVGSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKR 360

Query: 361 HDWTKIEKTRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 405
            ++ K EK ++ VE LES +    QAI      I+ L + ELYPQLV L  G
Sbjct: 361 AEYVKTEKAKKDVEKLESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKG 383

BLAST of Cucsat.G8166 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.6e-20
Identity = 137/513 (26.71%), Postives = 219/513 (42.69%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTE--- 60
           MGC AS +++E+ V+ C+ER++ MK+ +  R++ A +   YLR+L+ T A L +F +   
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQGHP 60

Query: 61  -------SETLELEDTIYGLASPPSPPPP-------LPP----------SPPPPPP---- 120
                  +  + L      LA  P+PPPP       LPP          +PPPPPP    
Sbjct: 61  SLAVSHHTAPVLLTTAAPALAPTPTPPPPSSTASSSLPPPTPLLPKHQQAPPPPPPTQSH 120

Query: 121 -------------------------------------------------------FSP-- 180
                                                                  F P  
Sbjct: 121 QPPPPVAVRAPRGGPRRLKVPHILSDSSVASPARSSFRKPVVGTPSSSSAWDWENFYPPS 180

Query: 181 -------DLRKHGAEDAQK-DELAQEESV--------VIDEDE-DEDDHTPSPPILSSSW 240
                  D RK   E+A +  EL +EE          + +EDE D+DD      +    W
Sbjct: 181 PPDSEFFDRRKADLEEANRLRELEEEEKARGYLHPHHLKEEDEVDDDDDEREEEMHCGGW 240

Query: 241 EYWDPFEHSAVHQQKKSE--TVGPVEEENWAETRSEFEEEDKKEEAVEDVVNPVPTTLEQ 300
           E  D    S    + +SE   +G   E  +A  RSE+      E A      P+P  L +
Sbjct: 241 EDDDDHYASTTTSETRSEEGEMGNRSECGFA-ARSEYGGTAPSEYAAA----PLPLPLRR 300

Query: 301 RELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKASGGIKEIAVLIDVT 360
           R+  S    +SS  +  A +M M+     +TL  +V  ++EYF+KA+     ++ L++ +
Sbjct: 301 RDERSEAGDSSST-VTAAAEMRMVI--RHRTLAEIVAAIEEYFVKAAEAGNGVSELLEAS 360

Query: 361 VGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSS-----TLQFATDAAEFLGPNEPCRPG 402
                   NFR+ K+   +S  + ++LS  W+S        +  T+A E     E     
Sbjct: 361 RAQ--LDRNFRQLKKTVYHSNSLLSSLSSTWTSKPPLAVRYKLDTNALEM----ESMEGK 420

BLAST of Cucsat.G8166 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 8.3e-14
Identity = 126/539 (23.38%), Postives = 210/539 (38.96%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAAS +D E+ V+ C++R++LMK+ +  R   A +   Y R+L+ TG+ L  F   E 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  LELEDTIYG--LASPPSP----------PPPLPPSPPP----PPPFSPDL---------- 120
           L + D      L +PP P          PP   PSP P    PP  SP +          
Sbjct: 61  LSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSKQPSVMS 120

Query: 121 -----RKHGAEDAQKDELAQEESVVIDEDEDEDDHTP--------------SPPILSSSW 180
                R+      +   +  E S       +  +  P              +P   SS W
Sbjct: 121 TSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTYSATPSHASSVW 180

Query: 181 EYWDPFE---------HSAVHQQKKSETVGPVEEENWAETRSE---FEEEDKKEEAVEDV 240
             W+ F           +   Q+KK  +     +E+    RSE   F+   +K++  E +
Sbjct: 181 N-WENFYPPSPPDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDFFDTRKQKQKQFESM 240

Query: 241 VNPVPTTLE-QRELISC--------ISSTSSL---------------------------- 300
            N V    E +RE + C         S+TSS                             
Sbjct: 241 RNQVEEETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEFGSTV 300

Query: 301 --------HMKVA---------------------------------TDMGMISWKNKKTL 360
                   H + +                                  DM M+     + L
Sbjct: 301 RSNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGDIADMKMV--VRHRDL 360

Query: 361 GAVVKELDEYFLKASGGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWS 405
             ++  + E F KA+   ++++ +++  +G      +F + K+   +S+ + + LS  W+
Sbjct: 361 KEIIDAIKENFDKAAASGEQVSQMLE--LGRAELDRSFSQLKKTVIHSSSLLSNLSSTWT 420

BLAST of Cucsat.G8166 vs. NCBI nr
Match: XP_004135735.1 (protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Cucumis sativus] >KGN65935.1 hypothetical protein Csa_023308 [Cucumis sativus])

HSP 1 Score: 780 bits (2015), Expect = 4.97e-280
Identity = 404/404 (100.00%), Postives = 404/404 (100.00%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET
Sbjct: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120
           LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED
Sbjct: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120

Query: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180
           EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV
Sbjct: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180

Query: 181 EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS 240
           EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS
Sbjct: 181 EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS 240

Query: 241 GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG 300
           GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG
Sbjct: 241 GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG 300

Query: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK 360
           PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK
Sbjct: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK 360

Query: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404
           TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG
Sbjct: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404

BLAST of Cucsat.G8166 vs. NCBI nr
Match: XP_008444972.1 (PREDICTED: uncharacterized protein LOC103488153 [Cucumis melo] >KAA0054068.1 DUF632 domain-containing protein/DUF630 domain-containing protein [Cucumis melo var. makuwa] >TYJ96855.1 DUF632 domain-containing protein/DUF630 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 756 bits (1953), Expect = 1.33e-270
Identity = 390/404 (96.53%), Postives = 399/404 (98.76%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET
Sbjct: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120
           LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEES+VIDEDED
Sbjct: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESIVIDEDED 120

Query: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180
           EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV
Sbjct: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180

Query: 181 EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS 240
           EDVV+PVP +LEQRELIS ISSTSSLHMKVATDMG+ISWKNKKTLGAVVKELDEYFLKAS
Sbjct: 181 EDVVDPVPKSLEQRELISSISSTSSLHMKVATDMGLISWKNKKTLGAVVKELDEYFLKAS 240

Query: 241 GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG 300
            GIKEIAVLIDV+VGNDFPPHNFRENKRKRSNSAKVFNALSRRWSS++LQFATDA EFLG
Sbjct: 241 AGIKEIAVLIDVSVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSNSLQFATDAVEFLG 300

Query: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK 360
           PNEPC+PGAHCITLKKLYAAEQRLQKDIKEEEGTNLEY+KK LLLQKQEDEHHDWTKIEK
Sbjct: 301 PNEPCQPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYEKKALLLQKQEDEHHDWTKIEK 360

Query: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404
           TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG
Sbjct: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404

BLAST of Cucsat.G8166 vs. NCBI nr
Match: XP_038894510.1 (protein ROLLING AND ERECT LEAF 2 [Benincasa hispida])

HSP 1 Score: 711 bits (1835), Expect = 1.28e-252
Identity = 373/406 (91.87%), Postives = 383/406 (94.33%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET
Sbjct: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120
           LELE TIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDE AQEES VIDEDED
Sbjct: 61  LELEGTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDEFAQEESTVIDEDED 120

Query: 121 ED--DHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEE 180
           ED  DHTPSPPILSSSWEYWDPFEHSAVHQQKKSE VGPVEEENWAETRSEFEEE K+EE
Sbjct: 121 EDEDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSEIVGPVEEENWAETRSEFEEEVKEEE 180

Query: 181 AVEDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLK 240
            VEDVVNPVP +LEQ ELISCISSTS LHMKVATDMGMISWKNKKTLGAVVKELDEYFLK
Sbjct: 181 TVEDVVNPVPKSLEQGELISCISSTSCLHMKVATDMGMISWKNKKTLGAVVKELDEYFLK 240

Query: 241 ASGGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEF 300
           ASGGIKEIAVLID++VGN F  HNFRENKRKRSNSAKVFNALSRRWSS++LQF T A EF
Sbjct: 241 ASGGIKEIAVLIDISVGNGFQTHNFRENKRKRSNSAKVFNALSRRWSSNSLQFTTVAVEF 300

Query: 301 LGPNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKI 360
           LGPNEPCRPGAHCITLKKLYAAEQRLQ+DIKEEE TNLE++KK  LLQKQEDEH+DWTK 
Sbjct: 301 LGPNEPCRPGAHCITLKKLYAAEQRLQQDIKEEESTNLEHEKKASLLQKQEDEHYDWTKT 360

Query: 361 EKTRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404
           EKTR TVEGLESDIIRLRQAIGE CASILALMDEELYPQLVALTSG
Sbjct: 361 EKTRLTVEGLESDIIRLRQAIGERCASILALMDEELYPQLVALTSG 406

BLAST of Cucsat.G8166 vs. NCBI nr
Match: XP_022139972.1 (uncharacterized protein LOC111010751 [Momordica charantia])

HSP 1 Score: 681 bits (1756), Expect = 1.04e-240
Identity = 355/405 (87.65%), Postives = 379/405 (93.58%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAASSIDEEERV+AC+ERKKLMKQL+GFRKEFADSLLAYLRALKNTGATLRQFTESET
Sbjct: 1   MGCAASSIDEEERVQACKERKKLMKQLVGFRKEFADSLLAYLRALKNTGATLRQFTESET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120
           LELE TIYGLASPPSPPPPLPPSPPPPPPFSPDLRK+GAED QKDE AQEES+VIDEDE 
Sbjct: 61  LELEGTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKYGAEDTQKDEFAQEESIVIDEDE- 120

Query: 121 EDDHTPSP-PILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEA 180
            DDHTPS  PILSSSWEYWDPFE S VHQQKKSETVG VEEENWAETRSEFEEEDK+EEA
Sbjct: 121 -DDHTPSAHPILSSSWEYWDPFELSTVHQQKKSETVG-VEEENWAETRSEFEEEDKEEEA 180

Query: 181 VEDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKA 240
           VEDVVNPVP + +Q EL+SC S+T+SLH+K ATDMGMISWK+KK+LGA VKELDEYFLKA
Sbjct: 181 VEDVVNPVPKSPDQMELVSCDSATTSLHIKDATDMGMISWKSKKSLGATVKELDEYFLKA 240

Query: 241 SGGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFL 300
           S GIKEIAVLID++VGNDFPPHNFRENKRKRSNSAKVFNALSRRWSS+++QF TD AEF 
Sbjct: 241 SAGIKEIAVLIDISVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSNSIQFTTDTAEFC 300

Query: 301 GPNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIE 360
           GPNEPCRPGAHCITLKKLYAAEQRLQKD+KEEEGT LE++KK LLLQKQEDEH+DWTK E
Sbjct: 301 GPNEPCRPGAHCITLKKLYAAEQRLQKDVKEEEGTKLEHEKKALLLQKQEDEHYDWTKTE 360

Query: 361 KTRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404
           KTRQ VE LESDIIRL+QAIG HCASILALMDEELYPQLVALTSG
Sbjct: 361 KTRQNVESLESDIIRLQQAIGGHCASILALMDEELYPQLVALTSG 402

BLAST of Cucsat.G8166 vs. NCBI nr
Match: XP_023520076.1 (nitrate regulatory gene2 protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 674 bits (1740), Expect = 2.35e-238
Identity = 354/404 (87.62%), Postives = 374/404 (92.57%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAASSIDEEERVKAC+ERKKLMKQLIG RKEFAD+LLAYLRALKNTGATLRQFTESET
Sbjct: 1   MGCAASSIDEEERVKACKERKKLMKQLIGSRKEFADTLLAYLRALKNTGATLRQFTESET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120
           LELE TIYGLASPPSPPPPLPPSPPPPPP SPDLRKHGAEDAQ      E S+VIDE+E 
Sbjct: 61  LELEGTIYGLASPPSPPPPLPPSPPPPPPLSPDLRKHGAEDAQ------EGSIVIDEEE- 120

Query: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180
            DDH+ SPPILSSSWEYWDPFE SAVH+QKKSE VGPVEEENWAETRSEFEEEDKKEE V
Sbjct: 121 -DDHSSSPPILSSSWEYWDPFELSAVHEQKKSEIVGPVEEENWAETRSEFEEEDKKEETV 180

Query: 181 EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS 240
           EDVVNPV  +LEQREL+SCISSTSSLHMKVATDMGMISWK KKTLGAVVKELDEYFLKAS
Sbjct: 181 EDVVNPVAKSLEQRELVSCISSTSSLHMKVATDMGMISWKRKKTLGAVVKELDEYFLKAS 240

Query: 241 GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG 300
           GGIKEIAVLID++ GNDFPP +FRE+KRKRSNSAKVFNALSRRWSS++LQF TD  EFLG
Sbjct: 241 GGIKEIAVLIDISAGNDFPPLHFRESKRKRSNSAKVFNALSRRWSSNSLQFTTDTVEFLG 300

Query: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK 360
           PNEPCRPGAHCITLKKLYAAEQRLQKDIKEE+GT LE++KK LLLQKQE+EH+DWTK EK
Sbjct: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEKGTILEHEKKALLLQKQEEEHYDWTKTEK 360

Query: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404
           TRQ VE LESDI+RLRQAIGE CASILALMDEELYPQLVALTSG
Sbjct: 361 TRQAVESLESDIVRLRQAIGEDCASILALMDEELYPQLVALTSG 396

BLAST of Cucsat.G8166 vs. ExPASy TrEMBL
Match: A0A0A0LVJ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G538820 PE=4 SV=1)

HSP 1 Score: 780 bits (2015), Expect = 2.41e-280
Identity = 404/404 (100.00%), Postives = 404/404 (100.00%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET
Sbjct: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120
           LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED
Sbjct: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120

Query: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180
           EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV
Sbjct: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180

Query: 181 EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS 240
           EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS
Sbjct: 181 EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS 240

Query: 241 GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG 300
           GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG
Sbjct: 241 GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG 300

Query: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK 360
           PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK
Sbjct: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK 360

Query: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404
           TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG
Sbjct: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404

BLAST of Cucsat.G8166 vs. ExPASy TrEMBL
Match: A0A5D3BEJ0 (DUF632 domain-containing protein/DUF630 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold10G00100 PE=4 SV=1)

HSP 1 Score: 756 bits (1953), Expect = 6.45e-271
Identity = 390/404 (96.53%), Postives = 399/404 (98.76%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET
Sbjct: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120
           LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEES+VIDEDED
Sbjct: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESIVIDEDED 120

Query: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180
           EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV
Sbjct: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180

Query: 181 EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS 240
           EDVV+PVP +LEQRELIS ISSTSSLHMKVATDMG+ISWKNKKTLGAVVKELDEYFLKAS
Sbjct: 181 EDVVDPVPKSLEQRELISSISSTSSLHMKVATDMGLISWKNKKTLGAVVKELDEYFLKAS 240

Query: 241 GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG 300
            GIKEIAVLIDV+VGNDFPPHNFRENKRKRSNSAKVFNALSRRWSS++LQFATDA EFLG
Sbjct: 241 AGIKEIAVLIDVSVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSNSLQFATDAVEFLG 300

Query: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK 360
           PNEPC+PGAHCITLKKLYAAEQRLQKDIKEEEGTNLEY+KK LLLQKQEDEHHDWTKIEK
Sbjct: 301 PNEPCQPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYEKKALLLQKQEDEHHDWTKIEK 360

Query: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404
           TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG
Sbjct: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404

BLAST of Cucsat.G8166 vs. ExPASy TrEMBL
Match: A0A1S3BCH2 (uncharacterized protein LOC103488153 OS=Cucumis melo OX=3656 GN=LOC103488153 PE=4 SV=1)

HSP 1 Score: 756 bits (1953), Expect = 6.45e-271
Identity = 390/404 (96.53%), Postives = 399/404 (98.76%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET
Sbjct: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120
           LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEES+VIDEDED
Sbjct: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESIVIDEDED 120

Query: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180
           EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV
Sbjct: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180

Query: 181 EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS 240
           EDVV+PVP +LEQRELIS ISSTSSLHMKVATDMG+ISWKNKKTLGAVVKELDEYFLKAS
Sbjct: 181 EDVVDPVPKSLEQRELISSISSTSSLHMKVATDMGLISWKNKKTLGAVVKELDEYFLKAS 240

Query: 241 GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG 300
            GIKEIAVLIDV+VGNDFPPHNFRENKRKRSNSAKVFNALSRRWSS++LQFATDA EFLG
Sbjct: 241 AGIKEIAVLIDVSVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSNSLQFATDAVEFLG 300

Query: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK 360
           PNEPC+PGAHCITLKKLYAAEQRLQKDIKEEEGTNLEY+KK LLLQKQEDEHHDWTKIEK
Sbjct: 301 PNEPCQPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYEKKALLLQKQEDEHHDWTKIEK 360

Query: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404
           TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG
Sbjct: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404

BLAST of Cucsat.G8166 vs. ExPASy TrEMBL
Match: A0A6J1CFH1 (uncharacterized protein LOC111010751 OS=Momordica charantia OX=3673 GN=LOC111010751 PE=4 SV=1)

HSP 1 Score: 681 bits (1756), Expect = 5.02e-241
Identity = 355/405 (87.65%), Postives = 379/405 (93.58%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAASSIDEEERV+AC+ERKKLMKQL+GFRKEFADSLLAYLRALKNTGATLRQFTESET
Sbjct: 1   MGCAASSIDEEERVQACKERKKLMKQLVGFRKEFADSLLAYLRALKNTGATLRQFTESET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120
           LELE TIYGLASPPSPPPPLPPSPPPPPPFSPDLRK+GAED QKDE AQEES+VIDEDE 
Sbjct: 61  LELEGTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKYGAEDTQKDEFAQEESIVIDEDE- 120

Query: 121 EDDHTPSP-PILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEA 180
            DDHTPS  PILSSSWEYWDPFE S VHQQKKSETVG VEEENWAETRSEFEEEDK+EEA
Sbjct: 121 -DDHTPSAHPILSSSWEYWDPFELSTVHQQKKSETVG-VEEENWAETRSEFEEEDKEEEA 180

Query: 181 VEDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKA 240
           VEDVVNPVP + +Q EL+SC S+T+SLH+K ATDMGMISWK+KK+LGA VKELDEYFLKA
Sbjct: 181 VEDVVNPVPKSPDQMELVSCDSATTSLHIKDATDMGMISWKSKKSLGATVKELDEYFLKA 240

Query: 241 SGGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFL 300
           S GIKEIAVLID++VGNDFPPHNFRENKRKRSNSAKVFNALSRRWSS+++QF TD AEF 
Sbjct: 241 SAGIKEIAVLIDISVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSNSIQFTTDTAEFC 300

Query: 301 GPNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIE 360
           GPNEPCRPGAHCITLKKLYAAEQRLQKD+KEEEGT LE++KK LLLQKQEDEH+DWTK E
Sbjct: 301 GPNEPCRPGAHCITLKKLYAAEQRLQKDVKEEEGTKLEHEKKALLLQKQEDEHYDWTKTE 360

Query: 361 KTRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404
           KTRQ VE LESDIIRL+QAIG HCASILALMDEELYPQLVALTSG
Sbjct: 361 KTRQNVESLESDIIRLQQAIGGHCASILALMDEELYPQLVALTSG 402

BLAST of Cucsat.G8166 vs. ExPASy TrEMBL
Match: A0A6J1EIH1 (nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111434379 PE=4 SV=1)

HSP 1 Score: 667 bits (1722), Expect = 6.15e-236
Identity = 350/404 (86.63%), Postives = 373/404 (92.33%), Query Frame = 0

Query: 1   MGCAASSIDEEERVKACRERKKLMKQLIGFRKEFADSLLAYLRALKNTGATLRQFTESET 60
           MGCAASSIDEEERVKAC+ERKKLMKQLIG RKEFAD+LLAYLRALKNTGATLRQFTESET
Sbjct: 1   MGCAASSIDEEERVKACKERKKLMKQLIGSRKEFADALLAYLRALKNTGATLRQFTESET 60

Query: 61  LELEDTIYGLASPPSPPPPLPPSPPPPPPFSPDLRKHGAEDAQKDELAQEESVVIDEDED 120
           LELE TIYGLASPPSPPPPLPPSPPPPPP SPDLRKHGAEDAQ      E S+VIDE+E 
Sbjct: 61  LELEGTIYGLASPPSPPPPLPPSPPPPPPLSPDLRKHGAEDAQ------EGSIVIDEEE- 120

Query: 121 EDDHTPSPPILSSSWEYWDPFEHSAVHQQKKSETVGPVEEENWAETRSEFEEEDKKEEAV 180
            DDH+ SPPILSSSWEYWDPFE SAVH+QKKSE VGPVEEENWAETRSEFEEEDKKEE V
Sbjct: 121 -DDHSSSPPILSSSWEYWDPFELSAVHEQKKSEIVGPVEEENWAETRSEFEEEDKKEETV 180

Query: 181 EDVVNPVPTTLEQRELISCISSTSSLHMKVATDMGMISWKNKKTLGAVVKELDEYFLKAS 240
           E VVNPV  +LEQREL+SCISST SLHMKVATDMGMISWK KKTLGAVVKELDEYFLKAS
Sbjct: 181 EGVVNPVAKSLEQRELVSCISSTRSLHMKVATDMGMISWKRKKTLGAVVKELDEYFLKAS 240

Query: 241 GGIKEIAVLIDVTVGNDFPPHNFRENKRKRSNSAKVFNALSRRWSSSTLQFATDAAEFLG 300
           GGIKEIAVLID++ GNDFPP +FRE+KRKRSNSAKVFNALSRRWSS++LQF TD+ EFLG
Sbjct: 241 GGIKEIAVLIDISAGNDFPPLHFRESKRKRSNSAKVFNALSRRWSSNSLQFTTDSVEFLG 300

Query: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEEGTNLEYKKKVLLLQKQEDEHHDWTKIEK 360
           PNEPCRPGAHCITLKKLYAAEQRLQKDIKEE+GT LE++KK LLLQKQE+EH+DWTK EK
Sbjct: 301 PNEPCRPGAHCITLKKLYAAEQRLQKDIKEEKGTILEHEKKALLLQKQEEEHYDWTKTEK 360

Query: 361 TRQTVEGLESDIIRLRQAIGEHCASILALMDEELYPQLVALTSG 404
           TRQ VE LESD++RLRQAIGE CASILALMDEEL+PQLVALTSG
Sbjct: 361 TRQAVESLESDLVRLRQAIGEDCASILALMDEELHPQLVALTSG 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A178VBJ01.0e-3230.83Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9AQW11.6e-2026.71Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL... [more]
Q93YU88.3e-1423.38Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
XP_004135735.14.97e-280100.00protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Cucumis sativus] >KGN65935.1 hy... [more]
XP_008444972.11.33e-27096.53PREDICTED: uncharacterized protein LOC103488153 [Cucumis melo] >KAA0054068.1 DUF... [more]
XP_038894510.11.28e-25291.87protein ROLLING AND ERECT LEAF 2 [Benincasa hispida][more]
XP_022139972.11.04e-24087.65uncharacterized protein LOC111010751 [Momordica charantia][more]
XP_023520076.12.35e-23887.62nitrate regulatory gene2 protein-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0LVJ92.41e-280100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G538820 PE=4 SV=1[more]
A0A5D3BEJ06.45e-27196.53DUF632 domain-containing protein/DUF630 domain-containing protein OS=Cucumis mel... [more]
A0A1S3BCH26.45e-27196.53uncharacterized protein LOC103488153 OS=Cucumis melo OX=3656 GN=LOC103488153 PE=... [more]
A0A6J1CFH15.02e-24187.65uncharacterized protein LOC111010751 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1EIH16.15e-23686.63nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111434... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 123..157
NoneNo IPR availablePANTHERPTHR21450:SF21REDUCTASE SUBUNIT C, PUTATIVE (DUF630 AND DUF632)-RELATEDcoord: 1..249
NoneNo IPR availablePANTHERPTHR21450UNCHARACTERIZEDcoord: 1..249
IPR006867Domain of unknown function DUF632PFAMPF04782DUF632coord: 1..121
e-value: 2.3E-30
score: 106.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G8166.T2Cucsat.G8166.T2mRNA
Cucsat.G8166.T1Cucsat.G8166.T1mRNA
Cucsat.G8166.T6Cucsat.G8166.T6mRNA
Cucsat.G8166.T5Cucsat.G8166.T5mRNA
Cucsat.G8166.T3Cucsat.G8166.T3mRNA
Cucsat.G8166.T4Cucsat.G8166.T4mRNA
Cucsat.G8166.T9Cucsat.G8166.T9mRNA
Cucsat.G8166.T7Cucsat.G8166.T7mRNA
Cucsat.G8166.T10Cucsat.G8166.T10mRNA
Cucsat.G8166.T8Cucsat.G8166.T8mRNA
Cucsat.G8166.T11Cucsat.G8166.T11mRNA
Cucsat.G8166.T12Cucsat.G8166.T12mRNA
Cucsat.G8166.T13Cucsat.G8166.T13mRNA
Cucsat.G8166.T14Cucsat.G8166.T14mRNA