Cucsat.G592 (gene) Cucumber (B10) v3

Overview
NameCucsat.G592
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionGTP diphosphokinase
Locationctg1: 11522599 .. 11527655 (-)
RNA-Seq ExpressionCucsat.G592
SyntenyCucsat.G592
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCAGTGGCAGTGGCAGCGTCTTCTTCTTCTTCTTCCTCTTCACCTTCATTGAAAGCTCCTTTTGAACTTAACAATGGCTCTGCTCCTTCCATATTAAGCTTCAAGAATCCATAGCAATGGAGCTTTTCACTGCCATTAATCCTTCTCCAGACCTCCATTTCCACTCCCTCCGTTCTCTCACCGACACCCATCACCTTCCCCTTCCCAGTCTTCTCCTCCGTCACCCTCGCCGCCCACTCAGCTCCCTCTCCCGTTGCCGCCTTCTGCATTTGCGAGCTTCCTCCACTTCTGGCATCCCGGATCTTCCCACCTCCGTACCGGAGCAGGCCGGCGGAAAATTAGTTGTGGAACTCATTGGAGCCTTCAATGAGCTCACTGATAGGATGAATCTCACTTCCACTAGTTCGTCTGTGATCCTCTTTGTCACTCTTAAGCTCTCAATCCCCATCTTGCAGAGTTTGCCTCTGCTTCCTGATGGCCGCTCTCCTCTCTCCAAGGCCTTGTCTGTCGCTCTTATTCTCGCCGATCTTCAGGTTCTCTTGCTGTTGTTACTAATTAAGTTGCTATCTCTTCGATTCTTTCTTCTTGATTGTTATTGATTGGTGGATTGTGAGTGTTTGCTTACCTAACTATTATTCAATGCATAGTAGAGTTGAATTCAGAAACTTTTGGGGATTATGGTTAGAGGAGATTAGAGAGCTTTCTGCTAAAAGGTATAATTATATGTTTGTTAAAAACTATTTTAAAAATCAGGGGTTTGTCCAAGAATTCCCACATGGTGTATTATTGATTTGTTGATGGGTTTTCCACTGTTTTGTCGAATTAACAATTGTGTTCTACTTCTTCTTCTTCGTTTCCTTGTTCTTCTCAAGCTATACATGTGTTCAATTTAACTGTGACCGAATAGTTTGGTCCTACTACTTGGTCCATTCAGTTCCAATTCCGAATCCAATTTTTAACAATGCTTGACAAGCGTTTTATGTACTCTTGTTTCGGTTTTTTTATGGTTTTATGGACTTGGTTCTGTCTACAGCTTTGTTTTGGTGCAAAAAAAAAAAAGCACCCTTTAATCACGTTTAGTTTTCGATTGGAAATTTCTTTTGTAATGTGCTTAATTCGTTACATCATTCTGATATGTTTCTTATTTTTTAAAAAAAAAAACTTGCTTGACAAATGCCAATATTTTCTAACTAGGTGGTGTTGTCATCAGGAAAGCTCACCAGTTGCAATTTCAACTGCCAATTGCCATACATTTTTTTAGTTATGGAATAGACTTGATGTTAGATTCTTGGGGGATGTTGAAACTAGTAGACAAATAGATGGAACTTAATGCTTCTGTTGCTTATTTTTGCTTTATAATAGGTATGGTTCTTAATGGAATTGGAAGCTCTATACTTGCTGAATAGCCTTTTCTGTGAATTTTAGTCTGCACTTGATAGATTTGAGACTTTCAGCTATTGATAGATTTTAGAACAAACCATTATCTCAGATGGATGCAGAAGTTATATCTACTGGTATTCTTAGAGAGGTTATGGAGGCTGGGGGGATATCTCTTCAAGAAGTTAAAAACCAAATAGGTATAAGTACTGCTCATTTACTGCATGAGAGCTTACGAGTGAAGCACATTCCTTCGAGAGTCGATATCTTTGACGACGATAGTTCGGCTGCTTTAAGAAAGTTCTGTCTTACGTATTATGATATTAGGGCATTGATTTTAGACCTGGCTTTGAAGCTTGATATGATGAGAAACCTCCAATCATTGCCAAGATACCAGCAGCAGATGGTTTCTCTTGAGGTTTTGAAGATACATGCACCATTAGCATTTGCCGTTGGAGCTAATTTCTTATCACTTCAGTTGGAGGATCTCTCTTTCCGTTACTTGTTTCCTTGCTCTTACTTCTATGTGGATTCGTGGTTGCGAAGCCACGAGTCAGGAAGTAAGTCTTTGATTGAAACATACAAGGATCAGCTGGCACAATCCTTGAAGAATGATCCTATATTAACAAACATGGTGGAAGATTTTTCTGTGAAAGGTCGCTATAAAAGTCGATCTAGCACCATGAAGAAACTCCTTAAAGATGGTCGAAAACTTGAAGAAGTTAACGATATTCTAGGATTACGCGTCATATTGAAACCTAAGGCTGGGACAGATACATCAGAATCGGGTGAGAGAGCTTGCTACCGGGCAAGTGAAATTATAAAGTCACAGTGGAAAGAAATACCCCATAGGACTAAAGATTATATTGCAAGGCCAAAACCCAATGGTTACAAGAGCTTACACATGGCTGTGGATGTGAGCAACGAGAGCCAGACGAAACCGTTAATGGAAATTCAAATACGAACTACTGAGATGGACAAATTGGCAGCTGGTGGAACGGCATCACATTCACTATACAAGGGAGGTCTCACAGATCCAGAAGAAGTCAGTAACAATTAGTATCTATTTTAATGTTTTAATACACTTGGTCTTGTTCTATAAAATTTGCACAAGCTTGTTAAGTTTAAGACTGATGCGGATATTTGTCAATGTTTTAGGCGAAAAGGCTCAAGGCTAAAATGTTGGCTGCAGCTGAACTTGCTGCTCTACGTCTTAACGATTTTCCATCATCTAACTATAAAGGCATACATACCAACCAAAGAGGTCGAGTATTTGGGCTTCTTGACAAGAATGGAGACGGTAGAATAAGTATTGAGGAACTTGTGGATGTCATGGAAGACCTTGGTGTTGGTGCCCCCGGAGAAGATGCGCGAGAGATGATGCAACTTCTAGATTCGAATAGTGATGGTTCTCTTAGCTCTGATGAATTCGACTTTTTTCAAAAACAGGTAAATTCTTCACCTTGTTTCAAGATAGTGAAATGATTCTGATTGCATATTTAGATCCCTGTGCACATAACTCCCAAAAACATCTGTCTTTTTTGTTGTGAAACAAAAATGAAATGATTTTTTTTTATAATAGTAAGTGTTAAACCAATTATCACTAAACCAAAGCCTAGCTGTGATTAATCTACACTTATATTCATATTAGTGCGGGCTATTTTTAAATATAACAAAATATACAATCTATCTACGATAGATCAAGATAAACTACTATGTGTCTATCATGGTCTATCATAGATCGATTGTGATATTTTACTATATTTGTAAATAATTTCAGAAATTTTGTCATTTAAAATAATCTCTCCAAGACGATTTATATCAATGTGTGGGACATGAAAATCGAGTCTTTAACCTTGAAGTTAATAGAATAAGTTTATGTTAATTGAGTTTGTTCATGTTGACAACAACCTCTCATGTCACAACCGTAATCTTTTATTTGAATTTTAATATTCAATCATATATTTTTCATCTTCACAAGGCCTTTCTCAAACCTGCATGTTCTTTACCCCAACGAATTAATATTGCATGTCTAAGATTACCAATGCAACTTGAAAAATGGAGAATTAGCTTTTAACAAAGTCAATTTGTTCTTAGGTCGAATTCATACGCAGCTTGGAGAACAGAGATGATCAATACAAGGTCATCCTAAATCATAAGCTCCAAAACGATGATGACACTGGTTTGATACAAGTCTATAGTGAAGAACTCGGCAATCGACTTGCCACATAGTCTTGACCGTGGACCCTCGATTTGTCTCAACAAGGGTAGGAAATTAAGTTCATTTGGAGTCACATATCTAGCCTCATTATTGTGAATAGATTGTTTATAAAGTTGAGAAGCAAGCCAATTCCTGTGCAACATTGTCATTTGGAGGTCTAATATTTTAATCAGAAAAAAAAATTGTAATCTACAAATGAAATGAAAAGAATCCAAATAGGATGAAGTAATAATGTATCGGAGATTTGTTTAGAAGATATAGAAAAGGAGGGAGAGATCTAATGATTATATTCTCAAGCTTCTATAATACAAATGAACTTTCTGTTCTTTTACATTTGATATTCGGTGTCTACATACAAAGGGGGATACACAAAGGAGGCTTCTTGCCATTTAATATTTGGCTCTATCTATTATCTACATTTAAACCCACTTATGCACCAATCAAAGGCCGTGCTTGACATCAACCCAAATTATAAAATAATAATAACAACAATAATTCTAATAAAAATCCTATAATTGGGTATAGATTTCTTCTAACATGTACTGTTTAATGCAAACGAGCTTGAATTTTGCCGTTCGAGCTCTGGGTTTGTGCCCAAATCACTTGACAGTCTTTCACACAACTGCAAGGTTTGAATGGAATTGAATGTTAGAAGTTAAAGGAAGATGTCAGAAGGACTTGGCACAAGTTTTTTCCAAATTTAGTACTTTGCAAGGATTGGAATGATGTTCCAAGTGATGAAAATGTATTTTTTTAGAGTAAAAGACGTAGTTTTAAGCACTTAAAATAGACAGACATTGAAACTAGCCTGTCCTATCTTGTACCTGTCTGTAAGCTGACGAGTCTCCAAAAGATTTTTTCAGTTCAACTACATGAAGCAGTGGGCGTATCTCAAAGACCTGAAATCATAACTCACCAGATTTGGTAATGGGGAGTAACTGTTAGCATTAGGTTCCATGAAAATTTAATTAGTAACTCGAATGAAATTTTTTTTTACCTCTGCTACAAATGATAGATTACTTAGGCTTTTTTGACTTCTTATCTCTTGAATCAATTTCAACTAAAGACGCAGAAAAGGAAAGATATGTTAGAGTAATCATGTTGTGAGAAAGAGAAACGGGGCATGTTACTTTGAAACGGGGCATGTTGCATGTTACTTTGAAACGGGGCATGTTACTTTGTAACCTCATGGCAAATAGGTCGCAAATTGTAGGGTCCGTTTGTCATATAAAATTGCGGACAACGATGGCCACAAGCTTTCAACTAATCCATTATCATGGAGGGGTTAACGATAATGACATCTACTAGATTCTTTCGATGCTAAGAATTGAGTACCAACCTTTCCACTTCTCTTTTGAACTCGAAATCCCATATCCGTTGCGATAACCTCGATTCTCTCAAGCAAGTCTTTGGCAGAACGATTTGAGGTAAATCTGATCTTCCTCTCGGAGACATCCT

Coding sequence (CDS)

ATGGATGCAGAAGTTATATCTACTGGTATTCTTAGAGAGGTTATGGAGGCTGGGGGGATATCTCTTCAAGAAGTTAAAAACCAAATAGGTATAAGTACTGCTCATTTACTGCATGAGAGCTTACGAGTGAAGCACATTCCTTCGAGAGTCGATATCTTTGACGACGATAGTTCGGCTGCTTTAAGAAAGTTCTGTCTTACGTATTATGATATTAGGGCATTGATTTTAGACCTGGCTTTGAAGCTTGATATGATGAGAAACCTCCAATCATTGCCAAGATACCAGCAGCAGATGGTTTCTCTTGAGGTTTTGAAGATACATGCACCATTAGCATTTGCCGTTGGAGCTAATTTCTTATCACTTCAGTTGGAGGATCTCTCTTTCCGTTACTTGTTTCCTTGCTCTTACTTCTATGTGGATTCGTGGTTGCGAAGCCACGAGTCAGGAAGTAAGTCTTTGATTGAAACATACAAGGATCAGCTGGCACAATCCTTGAAGAATGATCCTATATTAACAAACATGGTGGAAGATTTTTCTGTGAAAGGTCGCTATAAAAGTCGATCTAGCACCATGAAGAAACTCCTTAAAGATGGTCGAAAACTTGAAGAAGTTAACGATATTCTAGGATTACGCGTCATATTGAAACCTAAGGCTGGGACAGATACATCAGAATCGGGTGAGAGAGCTTGCTACCGGGCAAGTGAAATTATAAAGTCACAGTGGAAAGAAATACCCCATAGGACTAAAGATTATATTGCAAGGCCAAAACCCAATGGTTACAAGAGCTTACACATGGCTGTGGATGTGAGCAACGAGAGCCAGACGAAACCGTTAATGGAAATTCAAATACGAACTACTGAGATGGACAAATTGGCAGCTGGTGGAACGGCATCACATTCACTATACAAGGGAGGTCTCACAGATCCAGAAGAAGCGAAAAGGCTCAAGGCTAAAATGTTGGCTGCAGCTGAACTTGCTGCTCTACGTCTTAACGATTTTCCATCATCTAACTATAAAGGCATACATACCAACCAAAGAGGTCGAGTATTTGGGCTTCTTGACAAGAATGGAGACGGTAGAATAAGTATTGAGGAACTTGTGGATGTCATGGAAGACCTTGGTGTTGGTGCCCCCGGAGAAGATGCGCGAGAGATGATGCAACTTCTAGATTCGAATAGTGATGGTTCTCTTAGCTCTGATGAATTCGACTTTTTTCAAAAACAGGTCGAATTCATACGCAGCTTGGAGAACAGAGATGATCAATACAAGGTCATCCTAAATCATAAGCTCCAAAACGATGATGACACTGGTTTGATACAAGTCTATAGTGAAGAACTCGGCAATCGACTTGCCACATAG

Protein sequence

MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAALRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLSLQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSVKGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQWKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHSLYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGRISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDDQYKVILNHKLQNDDDTGLIQVYSEELGNRLAT
Homology
BLAST of Cucsat.G592 vs. ExPASy Swiss-Prot
Match: Q84R11 (Probable GTP diphosphokinase CRSH, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRSH PE=2 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 1.8e-171
Identity = 315/457 (68.93%), Postives = 376/457 (82.28%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS  IL EV++A  IS+ EV++ IG  TAHLLHE  RVK+IP +VD+ DD+++A+
Sbjct: 128 MDAEVISASILSEVVDANAISIYEVRDHIGTGTAHLLHEIFRVKNIPFKVDVLDDETAAS 187

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRKF LTYYDIRA+I+DL  KLD MR+L  LPRY+QQ++SLEVLKI++PLA AVGAN LS
Sbjct: 188 LRKFYLTYYDIRAVIMDLVSKLDEMRHLDHLPRYRQQILSLEVLKIYSPLAHAVGANHLS 247

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           L+LED+SFRYLFPCSY Y+DSWLR HE+GSK LI+ YK+QL +SLK+D +L  MV D  +
Sbjct: 248 LELEDISFRYLFPCSYIYLDSWLRGHENGSKPLIDVYKEQLHRSLKDDLVLAEMVNDVYI 307

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSR S MKKLL+DGRK EEVND+LGLRVIL P +  +  E GE+ACYR SEII+S 
Sbjct: 308 KGRYKSRYSMMKKLLRDGRKPEEVNDVLGLRVILMPNSVVNDVEVGEKACYRTSEIIRSL 367

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIARPK NGY+SLHMAVDVS+  Q +PLMEIQIRT +MD  A  GTASHS
Sbjct: 368 WKEIPHRTKDYIARPKENGYRSLHMAVDVSDSDQIRPLMEIQIRTMDMDGSANAGTASHS 427

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIH--TNQRGRVFGLLDKNGD 360
           LYKGGLTDP+EAKRLKA MLAAA+LAA+RL D  S+ ++     TNQR RVF LLDKNGD
Sbjct: 428 LYKGGLTDPKEAKRLKAIMLAAADLAAIRLKDISSNKHQSFKTTTNQRDRVFCLLDKNGD 487

Query: 361 GRISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENR 420
           G ISIEEL++VME+L  GAPGEDA EMMQLLDSNSDGSLSSDEFD FQKQVEF+R  E+R
Sbjct: 488 GMISIEELMEVMEEL--GAPGEDAEEMMQLLDSNSDGSLSSDEFDTFQKQVEFMRKWEDR 547

Query: 421 DDQYKVILN---HKLQNDDDTGLIQVYSEELGNRLAT 453
           D++YK +L+   H L + D TGLIQ+Y++EL +RL+T
Sbjct: 548 DNEYKSLLDEKLHDLPHQDTTGLIQLYNKELEDRLST 582

BLAST of Cucsat.G592 vs. ExPASy Swiss-Prot
Match: Q6ATB4 (Probable GTP diphosphokinase CRSH1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CRSH1 PE=2 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 1.7e-164
Identity = 298/450 (66.22%), Postives = 372/450 (82.67%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GILRE ++AG IS+++VK++IGISTAHLLHESLR+KH PS++D+ DD+S++A
Sbjct: 135 MDAEVISAGILREALDAGAISMRDVKSEIGISTAHLLHESLRLKHAPSKLDVLDDESASA 194

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRKFCL+YYDIRA+IL+LALKLDMMR+L  LPRY Q++ SLEVLKI+APLA AVGA  LS
Sbjct: 195 LRKFCLSYYDIRAVILELALKLDMMRHLDCLPRYLQRIKSLEVLKIYAPLAHAVGAGNLS 254

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           L+LEDLSFRYLFP SY ++D WLRS E+ +K LI++YK+QL Q+LK+D  L+ +V+D S+
Sbjct: 255 LELEDLSFRYLFPHSYDHIDQWLRSQETENKLLIDSYKEQLLQALKDDDELSQIVQDISI 314

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           +GRYKSR STMKKL+KDGRK EEVNDIL LRVIL+P+    + + G RAC+R  EII++ 
Sbjct: 315 QGRYKSRFSTMKKLVKDGRKPEEVNDILALRVILEPRCDGSSLDWGPRACHRTHEIIQAM 374

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKE+P RTK+Y+ RPK NGY+SLH+A+DVS   + +PLMEIQIRT EM K A GG ASHS
Sbjct: 375 WKEVPGRTKNYVTRPKENGYQSLHVAIDVSEPGKMRPLMEIQIRTKEMHKFAVGGEASHS 434

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDP EAKRLKA MLAAAELAA+RL D P+S+     +N   R F  LDKNGDGR
Sbjct: 435 LYKGGLTDPGEAKRLKAIMLAAAELAAMRLRDLPASDQG--DSNCTNRAFCQLDKNGDGR 494

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEEL +VMEDLG G  G+DA+E+M LLD+NSDGSLSSDEF+ FQ+Q+E +RSL+++DD
Sbjct: 495 ISIEELTEVMEDLGAG--GKDAKELMHLLDANSDGSLSSDEFEAFQRQIELMRSLDDKDD 554

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRL 451
           +Y+ IL  KLQ  D  GLIQVY ++LG++L
Sbjct: 555 RYRKILKEKLQTIDSAGLIQVYRKQLGDKL 580

BLAST of Cucsat.G592 vs. ExPASy Swiss-Prot
Match: Q75IS2 (Probable GTP diphosphokinase CRSH3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CRSH3 PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 6.2e-135
Identity = 261/463 (56.37%), Postives = 334/463 (72.14%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS G++R  ++ G +++ +V+ Q+G S A L+ ESL+VK  PS VD+ D+++++A
Sbjct: 115 MDAEVISAGMVRGALDTGALAMADVEAQLGASAAGLVEESLKVKRAPSEVDVADEEAASA 174

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRK CL+ YDIRA+IL+LA+KLD M++L  LP++QQ+  SLEVLK+ A LA AVGA  LS
Sbjct: 175 LRKRCLSSYDIRAVILELAVKLDAMKHLDVLPKHQQRTTSLEVLKVFALLAHAVGAGELS 234

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           L+LEDLSF+ L+P +Y ++D WL S E   K +I   K++L ++L  D  L   V    V
Sbjct: 235 LELEDLSFQRLYPQAYAHIDQWLSSQEDDCKRVIAASKEELLRALTADDELRCTVTGVDV 294

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPK---AGTDTSESGERACYRASEII 240
            GRYKSR STMKKL+KDGR+ E+VNDILG+RVIL P+    G    + G+RAC R  E+I
Sbjct: 295 MGRYKSRFSTMKKLVKDGRRPEDVNDILGMRVILDPRPGGGGGGDGDGGDRACLRTHEVI 354

Query: 241 KSQWKEIPHRTKDYIARPKPNGYKSLHMAVDVSN---ESQTKPLMEIQIRTTEMDKLAAG 300
           K+ WK++P RTKDYI RPK NGY+SLH+AVD+S    E + +PLMEIQ+RT EMD  A G
Sbjct: 355 KAMWKDVPARTKDYITRPKGNGYRSLHVAVDMSEPGPEGKKRPLMEIQVRTREMDMAAVG 414

Query: 301 GTASHSLYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSN-------YKGIHTNQRG 360
           G ASH+LYKGGLTDPEEAKRLKA MLAAAE+AA  L D P+ +                 
Sbjct: 415 GQASHALYKGGLTDPEEAKRLKAIMLAAAEVAAQHLRDEPAGDGGQTTAAASAATAGNVE 474

Query: 361 RVFGLLDKNGDGRISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQK 420
           R F LLDKNGDGRIS+EEL ++MEDLG G  G DA E+M+LLD+NSDGSLSSDEF  FQK
Sbjct: 475 RAFQLLDKNGDGRISMEELTEIMEDLGAG--GHDAEELMRLLDANSDGSLSSDEFALFQK 534

Query: 421 QVEFIRSLENRDDQYKVILNHKLQNDDDTGLIQVYSEELGNRL 451
           +V+    LEN+DD+YK IL  KLQ  DDTGLI VY + L ++L
Sbjct: 535 RVKLKTKLENKDDEYKEILKQKLQKVDDTGLIHVYRKNLSDKL 575

BLAST of Cucsat.G592 vs. ExPASy Swiss-Prot
Match: Q6ATB2 (Probable GTP diphosphokinase CRSH2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CRSH2 PE=2 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 1.8e-126
Identity = 252/464 (54.31%), Postives = 327/464 (70.47%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GI+R+ M+AG +++ + + Q+G   A LL ESL VK+ PSRVD+ D+++++A
Sbjct: 103 MDAEVISAGIVRQAMDAGAVAMADAEAQLGPGAAALLLESLDVKNAPSRVDVADEEAASA 162

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           +R   L+ YD+RA+IL+LA++LD M++L  +P++QQ+  SLEVLK+ APLA AVGA  LS
Sbjct: 163 VRNRILSGYDVRAVILELAIRLDAMKHLDGVPKHQQRTTSLEVLKVFAPLAHAVGAGALS 222

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
            +LEDLSF  L+P +Y  VD WL   E   K ++ T KD L Q+L  D  L + V  F V
Sbjct: 223 KELEDLSFWRLYPQAYAQVDQWLSGQEDDCKRVLATCKDDLLQALAADDELRHTVAGFDV 282

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSR S MKKL+KDGR+ E+V+DILG+RVIL  +AG   +  G RAC R  E+IK  
Sbjct: 283 KGRYKSRFSAMKKLVKDGRRPEDVHDILGMRVILDHRAG---AGDGHRACIRTHEVIKGM 342

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSN---ESQTKPLMEIQIRTTEMDKLAAGGTA 300
           WK++P RTKDYIARPK +GY+SLH+AVD+S    E + +PLME+QIRT EM+  A  G  
Sbjct: 343 WKDVPARTKDYIARPKGDGYRSLHIAVDMSEPGPEGKKRPLMEVQIRTKEMNDAAVFG-- 402

Query: 301 SHSLYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYK---------GIHTNQRGR 360
            H+LYKG L DPEEAKRLK  MLAAAE+AA  L D P++  +                 R
Sbjct: 403 -HALYKGCLADPEEAKRLKDIMLAAAEVAAQHLRDEPATGDQTGVPAAAAAAASAGNIER 462

Query: 361 VFGLLDKNGDGRISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQ 420
            F LLDKNGDGRIS+EEL ++MEDLG G  G+DA E+M+LLD N+DGSLSSDEF  FQK+
Sbjct: 463 AFRLLDKNGDGRISMEELTELMEDLGAG--GKDAEELMRLLDDNNDGSLSSDEFALFQKR 522

Query: 421 VEFIRSLENRDDQYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 453
           VE    LE++DD+YK IL  KLQ  DDTGLI VY + L ++L +
Sbjct: 523 VELKAKLEDKDDEYKEILRQKLQKVDDTGLIHVYRKNLSDKLVS 558

BLAST of Cucsat.G592 vs. ExPASy Swiss-Prot
Match: Q5HNR8 (GTP pyrophosphokinase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=relA PE=3 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 6.8e-33
Identity = 99/327 (30.28%), Postives = 167/327 (51.07%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           +D   I  G L +V+E    + ++VK+      A ++    ++K +  R    ++  +  
Sbjct: 66  LDGPTIVAGFLHDVIEDTSYTFEDVKDMFNEEIARIVDGVTKLKKVKYRSK--EEQQAEN 125

Query: 61  LRK-FCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFL 120
            RK F     D+R +++ LA +L  MR L+++PR +Q  +S E L+I+APLA  +G N +
Sbjct: 126 HRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQVRISKETLEIYAPLAHRLGINTI 185

Query: 121 SLQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFS 180
             +LED + RY+    YF + + ++      +S  E Y       +KN+  +T M     
Sbjct: 186 KWELEDTALRYIDSVQYFRIVNLMKK----KRSEREAYITNAINKIKNE--MTKMNLSGE 245

Query: 181 VKGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKS 240
           + GR K   S  +K++K  ++ +++ D+L +R+I+          +    CY    ++ +
Sbjct: 246 INGRPKHIYSIYRKMIKQKKQFDQIFDLLAIRIIV----------NSINDCYATLGLVHT 305

Query: 241 QWKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASH 300
            WK +P R KDYIA PK N Y+SLH  V   N       +EIQIRT EM ++A  G A+H
Sbjct: 306 LWKPMPGRFKDYIAMPKQNMYQSLHTTVVGPNGDP----LEIQIRTHEMHEIAEHGVAAH 365

Query: 301 SLYKGGLTDPEEAKRLKAKMLAAAELA 327
             YK G T  ++ +  + K+    ELA
Sbjct: 366 WAYKEGKTVNQKTQDFQNKLNWLKELA 370

BLAST of Cucsat.G592 vs. NCBI nr
Match: XP_004147635.1 (probable GTP diphosphokinase CRSH, chloroplastic [Cucumis sativus] >KAE8650414.1 hypothetical protein Csa_010316 [Cucumis sativus])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 452/452 (100.00%), Postives = 452/452 (100.00%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA
Sbjct: 140 MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 199

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS
Sbjct: 200 LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 259

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV
Sbjct: 260 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 319

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ
Sbjct: 320 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 379

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS
Sbjct: 380 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 439

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR
Sbjct: 440 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 499

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD
Sbjct: 500 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 559

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 452
           QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT
Sbjct: 560 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 591

BLAST of Cucsat.G592 vs. NCBI nr
Match: XP_008439006.1 (PREDICTED: probable GTP diphosphokinase CRSH, chloroplastic isoform X2 [Cucumis melo] >ADN34045.1 guanosine-3'5'-bis(diphosphate) 3'-pyrophosphohydrolase [Cucumis melo subsp. melo] >KAA0052146.1 putative GTP diphosphokinase CRSH [Cucumis melo var. makuwa] >TYK05111.1 putative GTP diphosphokinase CRSH [Cucumis melo var. makuwa])

HSP 1 Score: 846 bits (2186), Expect = 1.98e-306
Identity = 440/452 (97.35%), Postives = 442/452 (97.79%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA
Sbjct: 140 MDAEVISAGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 199

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS
Sbjct: 200 LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 259

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           LQLEDLSFRYLFPCSYFYVDSWLRSHESGS SLIETYKDQLAQSLKNDPILTNMVED SV
Sbjct: 260 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSTSLIETYKDQLAQSLKNDPILTNMVEDISV 319

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTD    GERACYRA EIIKSQ
Sbjct: 320 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDM---GERACYRACEIIKSQ 379

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIA PKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS
Sbjct: 380 WKEIPHRTKDYIASPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 439

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNY+GIHTNQRGRVFGLLDKNGDGR
Sbjct: 440 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYRGIHTNQRGRVFGLLDKNGDGR 499

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD
Sbjct: 500 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 559

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 452
           QYK ILNHKLQNDDD+GLIQVYSEELGNRLAT
Sbjct: 560 QYKAILNHKLQNDDDSGLIQVYSEELGNRLAT 588

BLAST of Cucsat.G592 vs. NCBI nr
Match: XP_008439005.1 (PREDICTED: probable GTP diphosphokinase CRSH, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 846 bits (2186), Expect = 3.71e-305
Identity = 440/452 (97.35%), Postives = 442/452 (97.79%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA
Sbjct: 220 MDAEVISAGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 279

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS
Sbjct: 280 LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 339

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           LQLEDLSFRYLFPCSYFYVDSWLRSHESGS SLIETYKDQLAQSLKNDPILTNMVED SV
Sbjct: 340 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSTSLIETYKDQLAQSLKNDPILTNMVEDISV 399

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTD    GERACYRA EIIKSQ
Sbjct: 400 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDM---GERACYRACEIIKSQ 459

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIA PKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS
Sbjct: 460 WKEIPHRTKDYIASPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 519

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNY+GIHTNQRGRVFGLLDKNGDGR
Sbjct: 520 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYRGIHTNQRGRVFGLLDKNGDGR 579

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD
Sbjct: 580 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 639

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 452
           QYK ILNHKLQNDDD+GLIQVYSEELGNRLAT
Sbjct: 640 QYKAILNHKLQNDDDSGLIQVYSEELGNRLAT 668

BLAST of Cucsat.G592 vs. NCBI nr
Match: XP_038891447.1 (probable GTP diphosphokinase CRSH, chloroplastic [Benincasa hispida])

HSP 1 Score: 826 bits (2133), Expect = 2.50e-298
Identity = 427/452 (94.47%), Postives = 441/452 (97.57%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GILREVMEAGGIS+QEV+NQ+GISTAHLLHESLRVKHIPSRVDIFDDDSSAA
Sbjct: 140 MDAEVISAGILREVMEAGGISIQEVRNQMGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 199

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LR+FCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQM+SLEVLKIHAPLAFAVGANFLS
Sbjct: 200 LRRFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMLSLEVLKIHAPLAFAVGANFLS 259

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQL QSLKNDP L +MVED SV
Sbjct: 260 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLTQSLKNDPELADMVEDISV 319

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGT+TSE GERACY+A EIIKSQ
Sbjct: 320 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTETSELGERACYQAREIIKSQ 379

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIP+RTKDYIARPKPNGYKSLHMAVDVS+ES+TKPLMEIQIRT EMDKLAAGGTASHS
Sbjct: 380 WKEIPNRTKDYIARPKPNGYKSLHMAVDVSDESETKPLMEIQIRTKEMDKLAAGGTASHS 439

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDPEEAKRLKAKMLAAAELAALRL DFPSSNYKGIHT+Q+GRVFGLLDKNGDGR
Sbjct: 440 LYKGGLTDPEEAKRLKAKMLAAAELAALRLKDFPSSNYKGIHTDQKGRVFGLLDKNGDGR 499

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD
Sbjct: 500 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 559

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 452
           QYK ILNHKLQNDD +GLIQVYSEELGNRLAT
Sbjct: 560 QYKAILNHKLQNDD-SGLIQVYSEELGNRLAT 590

BLAST of Cucsat.G592 vs. NCBI nr
Match: XP_023527746.1 (probable GTP diphosphokinase CRSH, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 821 bits (2121), Expect = 1.74e-296
Identity = 424/452 (93.81%), Postives = 437/452 (96.68%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GILREVME GGIS+QEVKNQIGISTAHLLHESLRVK IPSRVDIFDDDSSAA
Sbjct: 140 MDAEVISAGILREVMETGGISIQEVKNQIGISTAHLLHESLRVKQIPSRVDIFDDDSSAA 199

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LR+FCLTYYDIRALIL+LALKLD MR+LQ LPRYQQQM+SLEVLKIHAPLAFAVGANFLS
Sbjct: 200 LRRFCLTYYDIRALILELALKLDSMRHLQFLPRYQQQMLSLEVLKIHAPLAFAVGANFLS 259

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKD+L QSLKNDPILT MVED SV
Sbjct: 260 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDKLEQSLKNDPILTEMVEDISV 319

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKP++GTD+SE GERACY+A EIIKSQ
Sbjct: 320 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPRSGTDSSEMGERACYQAGEIIKSQ 379

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIARPKPNGYKSLHMAVDVS ES+TKPLMEIQIRTTEMDKLAAGGTASHS
Sbjct: 380 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSEESKTKPLMEIQIRTTEMDKLAAGGTASHS 439

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHT+QRGRVFGLLDKNGDGR
Sbjct: 440 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTDQRGRVFGLLDKNGDGR 499

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEELVDVMEDLGV A GEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD
Sbjct: 500 ISIEELVDVMEDLGVDAAGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 559

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 452
           QYK ILN KLQNDDD+GLIQVYSEELGNRLAT
Sbjct: 560 QYKAILNDKLQNDDDSGLIQVYSEELGNRLAT 591

BLAST of Cucsat.G592 vs. ExPASy TrEMBL
Match: A0A5D3BZL5 (GTP diphosphokinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1769G00150 PE=3 SV=1)

HSP 1 Score: 846 bits (2186), Expect = 9.60e-307
Identity = 440/452 (97.35%), Postives = 442/452 (97.79%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA
Sbjct: 140 MDAEVISAGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 199

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS
Sbjct: 200 LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 259

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           LQLEDLSFRYLFPCSYFYVDSWLRSHESGS SLIETYKDQLAQSLKNDPILTNMVED SV
Sbjct: 260 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSTSLIETYKDQLAQSLKNDPILTNMVEDISV 319

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTD    GERACYRA EIIKSQ
Sbjct: 320 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDM---GERACYRACEIIKSQ 379

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIA PKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS
Sbjct: 380 WKEIPHRTKDYIASPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 439

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNY+GIHTNQRGRVFGLLDKNGDGR
Sbjct: 440 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYRGIHTNQRGRVFGLLDKNGDGR 499

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD
Sbjct: 500 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 559

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 452
           QYK ILNHKLQNDDD+GLIQVYSEELGNRLAT
Sbjct: 560 QYKAILNHKLQNDDDSGLIQVYSEELGNRLAT 588

BLAST of Cucsat.G592 vs. ExPASy TrEMBL
Match: E5GC47 (GTP diphosphokinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 846 bits (2186), Expect = 9.60e-307
Identity = 440/452 (97.35%), Postives = 442/452 (97.79%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA
Sbjct: 140 MDAEVISAGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 199

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS
Sbjct: 200 LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 259

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           LQLEDLSFRYLFPCSYFYVDSWLRSHESGS SLIETYKDQLAQSLKNDPILTNMVED SV
Sbjct: 260 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSTSLIETYKDQLAQSLKNDPILTNMVEDISV 319

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTD    GERACYRA EIIKSQ
Sbjct: 320 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDM---GERACYRACEIIKSQ 379

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIA PKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS
Sbjct: 380 WKEIPHRTKDYIASPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 439

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNY+GIHTNQRGRVFGLLDKNGDGR
Sbjct: 440 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYRGIHTNQRGRVFGLLDKNGDGR 499

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD
Sbjct: 500 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 559

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 452
           QYK ILNHKLQNDDD+GLIQVYSEELGNRLAT
Sbjct: 560 QYKAILNHKLQNDDDSGLIQVYSEELGNRLAT 588

BLAST of Cucsat.G592 vs. ExPASy TrEMBL
Match: A0A1S3AXB7 (GTP diphosphokinase OS=Cucumis melo OX=3656 GN=LOC103483930 PE=3 SV=1)

HSP 1 Score: 846 bits (2186), Expect = 9.60e-307
Identity = 440/452 (97.35%), Postives = 442/452 (97.79%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA
Sbjct: 140 MDAEVISAGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 199

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS
Sbjct: 200 LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 259

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           LQLEDLSFRYLFPCSYFYVDSWLRSHESGS SLIETYKDQLAQSLKNDPILTNMVED SV
Sbjct: 260 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSTSLIETYKDQLAQSLKNDPILTNMVEDISV 319

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTD    GERACYRA EIIKSQ
Sbjct: 320 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDM---GERACYRACEIIKSQ 379

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIA PKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS
Sbjct: 380 WKEIPHRTKDYIASPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 439

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNY+GIHTNQRGRVFGLLDKNGDGR
Sbjct: 440 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYRGIHTNQRGRVFGLLDKNGDGR 499

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD
Sbjct: 500 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 559

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 452
           QYK ILNHKLQNDDD+GLIQVYSEELGNRLAT
Sbjct: 560 QYKAILNHKLQNDDDSGLIQVYSEELGNRLAT 588

BLAST of Cucsat.G592 vs. ExPASy TrEMBL
Match: A0A1S3AYD8 (GTP diphosphokinase OS=Cucumis melo OX=3656 GN=LOC103483930 PE=3 SV=1)

HSP 1 Score: 846 bits (2186), Expect = 1.80e-305
Identity = 440/452 (97.35%), Postives = 442/452 (97.79%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA
Sbjct: 220 MDAEVISAGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 279

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS
Sbjct: 280 LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 339

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           LQLEDLSFRYLFPCSYFYVDSWLRSHESGS SLIETYKDQLAQSLKNDPILTNMVED SV
Sbjct: 340 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSTSLIETYKDQLAQSLKNDPILTNMVEDISV 399

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTD    GERACYRA EIIKSQ
Sbjct: 400 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDM---GERACYRACEIIKSQ 459

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIA PKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS
Sbjct: 460 WKEIPHRTKDYIASPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 519

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNY+GIHTNQRGRVFGLLDKNGDGR
Sbjct: 520 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYRGIHTNQRGRVFGLLDKNGDGR 579

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD
Sbjct: 580 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 639

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 452
           QYK ILNHKLQNDDD+GLIQVYSEELGNRLAT
Sbjct: 640 QYKAILNHKLQNDDDSGLIQVYSEELGNRLAT 668

BLAST of Cucsat.G592 vs. ExPASy TrEMBL
Match: A0A6J1GUA2 (GTP diphosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111457600 PE=3 SV=1)

HSP 1 Score: 816 bits (2107), Expect = 1.14e-294
Identity = 422/452 (93.36%), Postives = 435/452 (96.24%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS GILREVMEAGGIS+QEVKNQIGISTAHLLHE LRVK IPSRVDIFDDDSSAA
Sbjct: 140 MDAEVISAGILREVMEAGGISIQEVKNQIGISTAHLLHELLRVKQIPSRVDIFDDDSSAA 199

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LR+FCLTYYDIRALIL+LALKLD MR+LQ LPRYQQQM+SLEVLKIHAPLAFAVGANFLS
Sbjct: 200 LRRFCLTYYDIRALILELALKLDSMRHLQFLPRYQQQMLSLEVLKIHAPLAFAVGANFLS 259

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKD+L QSLKNDPILT MVED SV
Sbjct: 260 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDKLEQSLKNDPILTEMVEDISV 319

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKP++G D+SE GERACY+A EIIKSQ
Sbjct: 320 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPRSGMDSSEMGERACYQAGEIIKSQ 379

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIARPKPNGYKSLHMAVDVS ES+TKPLMEIQIRTTEMDKLAAGGTASHS
Sbjct: 380 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSEESKTKPLMEIQIRTTEMDKLAAGGTASHS 439

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIHTNQRGRVFGLLDKNGDGR 360
           LYKGGLTDPEEAKRLKAKMLAAAELAALRL DFPSSNYKGIHT+QRGRVFGLLDKNGDGR
Sbjct: 440 LYKGGLTDPEEAKRLKAKMLAAAELAALRLKDFPSSNYKGIHTDQRGRVFGLLDKNGDGR 499

Query: 361 ISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 420
           ISIEELVDVMEDLGV A GEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD
Sbjct: 500 ISIEELVDVMEDLGVDAAGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENRDD 559

Query: 421 QYKVILNHKLQNDDDTGLIQVYSEELGNRLAT 452
           QYK ILN KLQNDDD+GLIQVYSEELGNRLAT
Sbjct: 560 QYKAILNDKLQNDDDSGLIQVYSEELGNRLAT 591

BLAST of Cucsat.G592 vs. TAIR 10
Match: AT3G17470.1 (Ca2+-activated RelA/spot homolog )

HSP 1 Score: 603.6 bits (1555), Expect = 1.3e-172
Identity = 315/457 (68.93%), Postives = 376/457 (82.28%), Query Frame = 0

Query: 1   MDAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA 60
           MDAEVIS  IL EV++A  IS+ EV++ IG  TAHLLHE  RVK+IP +VD+ DD+++A+
Sbjct: 128 MDAEVISASILSEVVDANAISIYEVRDHIGTGTAHLLHEIFRVKNIPFKVDVLDDETAAS 187

Query: 61  LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAVGANFLS 120
           LRKF LTYYDIRA+I+DL  KLD MR+L  LPRY+QQ++SLEVLKI++PLA AVGAN LS
Sbjct: 188 LRKFYLTYYDIRAVIMDLVSKLDEMRHLDHLPRYRQQILSLEVLKIYSPLAHAVGANHLS 247

Query: 121 LQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNMVEDFSV 180
           L+LED+SFRYLFPCSY Y+DSWLR HE+GSK LI+ YK+QL +SLK+D +L  MV D  +
Sbjct: 248 LELEDISFRYLFPCSYIYLDSWLRGHENGSKPLIDVYKEQLHRSLKDDLVLAEMVNDVYI 307

Query: 181 KGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRASEIIKSQ 240
           KGRYKSR S MKKLL+DGRK EEVND+LGLRVIL P +  +  E GE+ACYR SEII+S 
Sbjct: 308 KGRYKSRYSMMKKLLRDGRKPEEVNDVLGLRVILMPNSVVNDVEVGEKACYRTSEIIRSL 367

Query: 241 WKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAGGTASHS 300
           WKEIPHRTKDYIARPK NGY+SLHMAVDVS+  Q +PLMEIQIRT +MD  A  GTASHS
Sbjct: 368 WKEIPHRTKDYIARPKENGYRSLHMAVDVSDSDQIRPLMEIQIRTMDMDGSANAGTASHS 427

Query: 301 LYKGGLTDPEEAKRLKAKMLAAAELAALRLNDFPSSNYKGIH--TNQRGRVFGLLDKNGD 360
           LYKGGLTDP+EAKRLKA MLAAA+LAA+RL D  S+ ++     TNQR RVF LLDKNGD
Sbjct: 428 LYKGGLTDPKEAKRLKAIMLAAADLAAIRLKDISSNKHQSFKTTTNQRDRVFCLLDKNGD 487

Query: 361 GRISIEELVDVMEDLGVGAPGEDAREMMQLLDSNSDGSLSSDEFDFFQKQVEFIRSLENR 420
           G ISIEEL++VME+L  GAPGEDA EMMQLLDSNSDGSLSSDEFD FQKQVEF+R  E+R
Sbjct: 488 GMISIEELMEVMEEL--GAPGEDAEEMMQLLDSNSDGSLSSDEFDTFQKQVEFMRKWEDR 547

Query: 421 DDQYKVILN---HKLQNDDDTGLIQVYSEELGNRLAT 453
           D++YK +L+   H L + D TGLIQ+Y++EL +RL+T
Sbjct: 548 DNEYKSLLDEKLHDLPHQDTTGLIQLYNKELEDRLST 582

BLAST of Cucsat.G592 vs. TAIR 10
Match: AT4G02260.3 (RELA/SPOT homolog 1 )

HSP 1 Score: 131.7 bits (330), Expect = 1.5e-30
Identity = 94/307 (30.62%), Postives = 157/307 (51.14%), Query Frame = 0

Query: 1   MDAEVISTGILREVME-AGGISLQEVKNQIGISTAHLLHESLRVKHI-----PSRVDIFD 60
           +D E I  G+L + +E    I+ ++++ + G +  H++    +V  +      +  +   
Sbjct: 188 LDWESIVAGLLHDTVEDTNFITFEKIEEEFGATVRHIVEGETKVSKLGKLKCKTESETIQ 247

Query: 61  DDSSAALRKFCLTYYD-IRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFA 120
           D  +  LR+  L   D +R +I+ LA +L  MR L  +P ++Q  ++ E L++ APLA  
Sbjct: 248 DVKADDLRQMFLAMTDEVRVIIVKLADRLHNMRTLCHMPPHKQSSIAGETLQVFAPLAKL 307

Query: 121 VGANFLSLQLEDLSFRYLFPCSYFYVDSWLRS-HESGSKSLIETYKDQLAQSLKNDPILT 180
           +G   +  +LE+LSF Y+    Y  V S + + ++   K L E  +  L + +++D  L 
Sbjct: 308 LGMYSIKSELENLSFMYVSAEDYDRVTSRIANLYKEHEKELTEANR-ILVKKIEDDQFLD 367

Query: 181 NMVEDFSVKGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPK--AGTDTSESGERAC 240
            +  +  V+   K   S  K  LK    + + N I  LR+++KPK   G     S ++ C
Sbjct: 368 LVTVNTDVRSVCKETYSIYKAALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQIC 427

Query: 241 YRASEIIKSQWKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDK 298
           Y    ++   WK IP   KDYIA PKPNGY+SLH  V +    ++   +E+QIRT EMD 
Sbjct: 428 YHVLGLVHEIWKPIPRTVKDYIATPKPNGYQSLHTTV-IPFLYESMFRLEVQIRTEEMDL 487

BLAST of Cucsat.G592 vs. TAIR 10
Match: AT4G02260.2 (RELA/SPOT homolog 1 )

HSP 1 Score: 131.7 bits (330), Expect = 1.5e-30
Identity = 94/307 (30.62%), Postives = 157/307 (51.14%), Query Frame = 0

Query: 1   MDAEVISTGILREVME-AGGISLQEVKNQIGISTAHLLHESLRVKHI-----PSRVDIFD 60
           +D E I  G+L + +E    I+ ++++ + G +  H++    +V  +      +  +   
Sbjct: 188 LDWESIVAGLLHDTVEDTNFITFEKIEEEFGATVRHIVEGETKVSKLGKLKCKTESETIQ 247

Query: 61  DDSSAALRKFCLTYYD-IRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFA 120
           D  +  LR+  L   D +R +I+ LA +L  MR L  +P ++Q  ++ E L++ APLA  
Sbjct: 248 DVKADDLRQMFLAMTDEVRVIIVKLADRLHNMRTLCHMPPHKQSSIAGETLQVFAPLAKL 307

Query: 121 VGANFLSLQLEDLSFRYLFPCSYFYVDSWLRS-HESGSKSLIETYKDQLAQSLKNDPILT 180
           +G   +  +LE+LSF Y+    Y  V S + + ++   K L E  +  L + +++D  L 
Sbjct: 308 LGMYSIKSELENLSFMYVSAEDYDRVTSRIANLYKEHEKELTEANR-ILVKKIEDDQFLD 367

Query: 181 NMVEDFSVKGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPK--AGTDTSESGERAC 240
            +  +  V+   K   S  K  LK    + + N I  LR+++KPK   G     S ++ C
Sbjct: 368 LVTVNTDVRSVCKETYSIYKAALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQIC 427

Query: 241 YRASEIIKSQWKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDK 298
           Y    ++   WK IP   KDYIA PKPNGY+SLH  V +    ++   +E+QIRT EMD 
Sbjct: 428 YHVLGLVHEIWKPIPRTVKDYIATPKPNGYQSLHTTV-IPFLYESMFRLEVQIRTEEMDL 487

BLAST of Cucsat.G592 vs. TAIR 10
Match: AT3G14050.1 (RELA/SPOT homolog 2 )

HSP 1 Score: 129.0 bits (323), Expect = 9.4e-30
Identity = 97/311 (31.19%), Postives = 163/311 (52.41%), Query Frame = 0

Query: 2   DAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA- 61
           ++ V+  G+L + ++   +S   +    G   A L+    ++  +     +  ++++A  
Sbjct: 250 NSTVVVAGLLHDTIDDSFMSYDYILRNFGAGVADLVEGVSKLSQLSK---LARENNTACK 309

Query: 62  ------LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAV 121
                 L    L   D RA+++ LA +L  M+ L +L   +QQ  + E L+I APLA  +
Sbjct: 310 TVEADRLHTMFLAMADARAVLIKLADRLHNMKTLYALSPVKQQRFAKETLEIFAPLANRL 369

Query: 122 GANFLSLQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNM 181
           G +   +QLE+L F++L+P  +  + + L   +S  +++I +  ++L Q+LK   I  ++
Sbjct: 370 GISTWKVQLENLCFKHLYPNQHNEMSTML--EDSFDEAMITSAIEKLEQALKKAGISYHV 429

Query: 182 VEDFSVKGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRAS 241
                + GR+KS  S   K+LK    ++E++DI GLR+I+            E  CY+A 
Sbjct: 430 -----LCGRHKSLYSIYSKMLKKKLTVDEIHDIHGLRLIV----------DNEGDCYKAL 489

Query: 242 EIIKSQWKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAG 301
            ++ S W E+P + KDYI  PK NGY+SLH  V    ++ T PL E+QIRT EM   A  
Sbjct: 490 GVVHSLWSEVPGKLKDYITHPKFNGYQSLHTVV---MDNGTVPL-EVQIRTQEMHLQAEF 536

Query: 302 GTASHSLYKGG 306
           G A+H  YK G
Sbjct: 550 GFAAHWRYKEG 536

BLAST of Cucsat.G592 vs. TAIR 10
Match: AT1G54130.1 (RELA/SPOT homolog 3 )

HSP 1 Score: 128.3 bits (321), Expect = 1.6e-29
Identity = 97/311 (31.19%), Postives = 161/311 (51.77%), Query Frame = 0

Query: 2   DAEVISTGILREVMEAGGISLQEVKNQIGISTAHLLHESLRVKHIPSRVDIFDDDSSAA- 61
           ++ V+  GIL + ++   +S   +    G   A L+    ++  +     +  ++++A  
Sbjct: 254 NSTVVVAGILHDTLDDSFMSYDYILRTFGSGVADLVEGVSKLSQLSK---LARENNTACK 313

Query: 62  ------LRKFCLTYYDIRALILDLALKLDMMRNLQSLPRYQQQMVSLEVLKIHAPLAFAV 121
                 L    L   D RA+++ LA +L  M  L +LP  ++Q  + E L+I APLA  +
Sbjct: 314 TVEADRLHTMFLAMADARAVLIKLADRLHNMMTLYALPPVKRQRFAKETLEIFAPLANRL 373

Query: 122 GANFLSLQLEDLSFRYLFPCSYFYVDSWLRSHESGSKSLIETYKDQLAQSLKNDPILTNM 181
           G +   ++LE+L F++L P  +  +   L   +S  +++I +  ++L Q+LK + I  ++
Sbjct: 374 GISSWKVKLENLCFKHLHPDQHHEMSDML--EDSFDEAMITSAIEKLEQALKKEGISYHV 433

Query: 182 VEDFSVKGRYKSRSSTMKKLLKDGRKLEEVNDILGLRVILKPKAGTDTSESGERACYRAS 241
                V GR+KS  S   K+LK    ++E++DI GLR+I+            E+ CY+A 
Sbjct: 434 -----VSGRHKSLYSIYCKMLKKKLTMDEIHDIHGLRLIV----------DNEKDCYKAL 493

Query: 242 EIIKSQWKEIPHRTKDYIARPKPNGYKSLHMAVDVSNESQTKPLMEIQIRTTEMDKLAAG 301
            ++   W E+P + KDYI+ PK NGY+SLH  V       T PL E+QIRT EM   A  
Sbjct: 494 GVVHKLWSEVPGKLKDYISHPKFNGYQSLHTVV---MGDGTIPL-EVQIRTKEMHLQAEF 540

Query: 302 GTASHSLYKGG 306
           G A+H  YK G
Sbjct: 554 GFAAHWRYKEG 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84R111.8e-17168.93Probable GTP diphosphokinase CRSH, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q6ATB41.7e-16466.22Probable GTP diphosphokinase CRSH1, chloroplastic OS=Oryza sativa subsp. japonic... [more]
Q75IS26.2e-13556.37Probable GTP diphosphokinase CRSH3, chloroplastic OS=Oryza sativa subsp. japonic... [more]
Q6ATB21.8e-12654.31Probable GTP diphosphokinase CRSH2, chloroplastic OS=Oryza sativa subsp. japonic... [more]
Q5HNR86.8e-3330.28GTP pyrophosphokinase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) ... [more]
Match NameE-valueIdentityDescription
XP_004147635.10.0100.00probable GTP diphosphokinase CRSH, chloroplastic [Cucumis sativus] >KAE8650414.1... [more]
XP_008439006.11.98e-30697.35PREDICTED: probable GTP diphosphokinase CRSH, chloroplastic isoform X2 [Cucumis ... [more]
XP_008439005.13.71e-30597.35PREDICTED: probable GTP diphosphokinase CRSH, chloroplastic isoform X1 [Cucumis ... [more]
XP_038891447.12.50e-29894.47probable GTP diphosphokinase CRSH, chloroplastic [Benincasa hispida][more]
XP_023527746.11.74e-29693.81probable GTP diphosphokinase CRSH, chloroplastic [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A5D3BZL59.60e-30797.35GTP diphosphokinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1769... [more]
E5GC479.60e-30797.35GTP diphosphokinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3AXB79.60e-30797.35GTP diphosphokinase OS=Cucumis melo OX=3656 GN=LOC103483930 PE=3 SV=1[more]
A0A1S3AYD81.80e-30597.35GTP diphosphokinase OS=Cucumis melo OX=3656 GN=LOC103483930 PE=3 SV=1[more]
A0A6J1GUA21.14e-29493.36GTP diphosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111457600 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G17470.11.3e-17268.93Ca2+-activated RelA/spot homolog [more]
AT4G02260.31.5e-3030.62RELA/SPOT homolog 1 [more]
AT4G02260.21.5e-3030.62RELA/SPOT homolog 1 [more]
AT3G14050.19.4e-3031.19RELA/SPOT homolog 2 [more]
AT1G54130.11.6e-2931.19RELA/SPOT homolog 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007685RelA/SpoTSMARTSM00954RelA_SpoT_2coord: 182..306
e-value: 1.8E-40
score: 150.5
IPR007685RelA/SpoTPFAMPF04607RelA_SpoTcoord: 182..304
e-value: 1.1E-30
score: 106.3
IPR007685RelA/SpoTCDDcd05399NT_Rel-Spo_likecoord: 151..292
e-value: 2.81691E-32
score: 117.448
IPR002048EF-hand domainSMARTSM00054efh_1coord: 381..409
e-value: 0.73
score: 18.9
coord: 345..373
e-value: 0.015
score: 24.5
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 348..403
e-value: 9.9E-9
score: 35.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 377..412
score: 9.492233
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 348..376
score: 12.365459
IPR002048EF-hand domainCDDcd00051EFhcoord: 348..402
e-value: 9.96144E-12
score: 57.9429
IPR043519Nucleotidyltransferase superfamilyGENE3D3.30.460.10Beta Polymerase, domain 2coord: 134..285
e-value: 2.5E-36
score: 126.5
IPR043519Nucleotidyltransferase superfamilySUPERFAMILY81301Nucleotidyltransferasecoord: 133..326
NoneNo IPR availablePFAMPF13328HD_4coord: 1..115
e-value: 6.7E-12
score: 45.5
NoneNo IPR availableGENE3D1.10.238.10coord: 286..419
e-value: 3.1E-16
score: 61.8
NoneNo IPR availableGENE3D1.10.3210.10Hypothetical protein af1432coord: 1..131
e-value: 5.6E-18
score: 67.2
NoneNo IPR availablePANTHERPTHR21262GUANOSINE-3',5'-BIS DIPHOSPHATE 3'-PYROPHOSPHOHYDROLASEcoord: 1..441
NoneNo IPR availablePANTHERPTHR21262:SF12GTP DIPHOSPHOKINASE CRSH, CHLOROPLASTIC-RELATEDcoord: 1..441
NoneNo IPR availableSUPERFAMILY109604HD-domain/PDEase-likecoord: 1..131
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 390..402
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 354..366
IPR006674HD domainPROSITEPS51831HDcoord: 1..85
score: 16.753819
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 342..408

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G592.T12Cucsat.G592.T12mRNA
Cucsat.G592.T10Cucsat.G592.T10mRNA
Cucsat.G592.T11Cucsat.G592.T11mRNA
Cucsat.G592.T4Cucsat.G592.T4mRNA
Cucsat.G592.T5Cucsat.G592.T5mRNA
Cucsat.G592.T6Cucsat.G592.T6mRNA
Cucsat.G592.T7Cucsat.G592.T7mRNA
Cucsat.G592.T1Cucsat.G592.T1mRNA
Cucsat.G592.T2Cucsat.G592.T2mRNA
Cucsat.G592.T3Cucsat.G592.T3mRNA
Cucsat.G592.T8Cucsat.G592.T8mRNA
Cucsat.G592.T13Cucsat.G592.T13mRNA
Cucsat.G592.T9Cucsat.G592.T9mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015969 guanosine tetraphosphate metabolic process
biological_process GO:0016310 phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005525 GTP binding
molecular_function GO:0008728 GTP diphosphokinase activity
molecular_function GO:0016301 kinase activity