Cucsat.G4274 (gene) Cucumber (B10) v3

Overview
NameCucsat.G4274
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionProtein KTI12-like protein
Locationctg1170: 658444 .. 662429 (-)
RNA-Seq ExpressionCucsat.G4274
SyntenyCucsat.G4274
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATGAAAATAAGTAAAATTGAGCCCTAAACATATGTATGTATCTTCCTCTCACTTTCAAGTCTCACGTGCTTCACCTTCCGTCTTTAGCAGCTCAGCTCCGCCGCTGCCACTTTCCAGTTCGTTGTCGCCGCCGTTCTTCCGCTCGTGCCAGCGCCGCATCCCTCCTAACGGATCTTCACTGATTTTCCACTCGGTTGTTGCTACTGGTATTATCCCAATCTCCATCTATATCTATCTCTTCACTTCAAAAACATAAATTAGGGCTTTTCTAATGAAAAAATGGAATATTTGTTTTTGTTTTTGTTTTTCTTAATAACCATACCCGAATAGCAACGAAAATAGAAGAAACCCACCTTTTGATTTGCTGCATATGTCTGTCAATTGAGGCGTCCATATATCTCTTTGGTCTCTGCTGAAAATTTCCTTGCTTCTGCACCAATGAATTTTCCAGCTTCTCCCTCGTCAGTTGAAGTAATCCAATTCTCTTTTAATTCCAATGAGTGTTTTATTTTAATTGACTTCCTCAACCGTATTTATTTTTGTATATTTGTTGAAGCAGTACGCTGACCAGCGGTACTTTAAATTTACTGAAAATAAGAGCCTTCTCTATTCTTTGTTCTTCATGTTCTTGATTTAAAATATGTATATTTGGGGTAAAATATACATATTTTTGGAAGTGGAGGGCTCAAGGGACCTAAAGATTCTGCTTTTATATATGGTATGGATTTTTTTAGGATTCTCTTAATGTTTTTGGAGAGAAAACAATATCAATGATTCTATGAAATTTACAAATTGAGTAATGGTATGTTGAAAATTATAATTGTTGTAAGCAGAGACGTGTGAAGTGTGTACAACAATATGAACCTAGAAAAGGTTTCTTGTGAATCAAAGCTTCAGGAGATCTACCATTAAATTTTTGTTGTTGTTCCTTTAATTGCATATAATCCGATAGAAAGAAAGAAAAAGTTGTTGATGTTGAGCCGGAGTGCCTGTTTTGCCTACTTGAATGAATGGTCCTTGAACAGGCTGAGCTGCCAGTATTTTATGTTGAATGAGAATGGGAGTGTCAAAATCCAACAATTAAAGGCTGTTTGAAAGTGAACCCAAATCCTGTTTGAAAATTGGCAGTGTATGAAAAGATGGTCTTGAGTCTCATGATCAGAGCTGCATAAACAGCAGCAATTTGGGGAGAGACTGATCCAGGGGTGCTTTATTTGAATTGTGTTCCTTGTGTGGTTTCATTTTAGAATTTTAGGCTGATTTCCCATAAAAAGAATTTTAAGTGTCTTTTGAAAGAACCCCTTTCTGAATCAGTTTGTATTGTCTGGATGATTGCTTTCTGACATAGTTGATAGGCCACTGACTTTTTTCCCAATTCCCAGTTGACGTACTTACAGGGTGTAATGATTTCTATTAACTATTATTTAGCTTACCGACTGTTTTCATGACGAGAAATGATTTGTTTCCCAGTGGGCACAGTAGGTGTCTAATTGAGAGAGAGAGTGCTGAGTGTGTAATTTTCTGCTATTGATAAGGGAGATTGAGTTACTTGTTTCTGAATATGAGAAGTTTTGAGTTTATGGCCTTAACGCTTGACTTCTTGCTTTTAAAATCAAGATTTTGATAGAACACTGATACAATGTTTCTATTTTATTGATACCTTCAACAGAATTACAATATAAAAGAAGTAAAATAATAAGTGAAAAAAGAACCCTAATCGTCTGCCCTTCCTCTCTTTCAAGGAATAGAGAAATTTTGCAGCCTTATTTCTTCACCAAATTCTTCATACCCTTCTTCCCTCCCCAACTTCCTATTTATAACCAACCAACTACCAACAAATCTAATTACACTTTTACCCCTGTCCTATACTAATCTAGGTATCTAACAGATTTCCTCTATCCAAAACTTCTACCTCCATCCCTTATTTCGCCTGAAGTCTGTTCAATCAGCTGAACTTCAGATTACAAAAATTAGCCAAGGGCCACAAAGAATTTGTAGAATGGTGACAAATCCATTTAAATTCCGGTTTGGTGCAGCCACCACCCACACCAATCTTTTATACTGTTCTTTGGATCTGAAAGAGTAATCTGAACCTCTTTCTGTCATCTCTTGCCACTCTATATTCTGAATCTAGGTAGCTTCTCTGGCTTTTGTGTATTTCTTCACTTTGAAATGTTCATAGTAAATAATTATTGTGAGGAAATGAACGTGGCCAGACCTTAATTATTGAAAGCCAAGAATTGTTTGACTAAATACAACTAGCTCTTCATTTAAAGGAGTTCAATCATTAAGAGGTAAAAAGGAAGACATAGTTTCAATAACTAACTAAGAGGTCATGTATTCAATTCATAGTAGCCGCCTAGGAATTAATTTCCTACAAGTTTTCTTTCACCAAAATGTTGTTGGGTCAGACGAGTTGTCTCGTGAGATTAGTCGAGTTGTGCGTAAGCTAGTCCAGACACTCACAGATGTAATGGATGGAGTTCGACTCTTTGCTTTTGGGTGCTGTTATTTTACTCATAGCTATGTTTCTGTCTTTCTTATTGCATTTGCATCTGTAAAAAGTTTTCTTGTAGTGTGTGATATTCAATCATAATTTATTTGTGTTATTTCTCTGATCATTTCTTTTAATGGTTCTACTTATGTTATGGAATTACTTTTTTTTAATAAAAAAGACACATATTCTTACTCGAAAAGTTATTGATGTTTGTTGTTTTTTCTTTGACTATTAACAGTTGGAACTTCTCTGCTTGGAGTTCTCCATAAAATGGCGTTGGTTGTGATCTGTGGCCAGCCATGCAGTGGGAAGTCCACTGCTGCACTCTGCTTAGCTGAAGCTCTCAAGGAATCAAATGTAAAAGAAGCCATTAAGATTATCGATGAAACTTCTCTTCATTTGGATCGGAATCAGAGCTATGCCAACATGCCTGCTGAAAAAAATCTAAGAGGAGTTCTCAGATCTGAAGTTGATAGATCTGTATCTAAAGATAGCATTGTCATTGTAGACTCTTTGAACAGTATAAAGGGTTATCGATATGAACTGTGGTGTTTGGCTCGTGGAACAGGAATCAGGTATTGTGTGCTGTACTGCGACGTGGAAGAAACCAATTGTAGGAAGTGGAATGAAGAGCGTCGTGAGAAAGAAGAAGCGAGCTATGATGATAAGATATTCGAAGATCTTCTCAGAAGGTTTGAGAGGCCAAATAGTAGAAATAGATGGGACTCACCATTGTTTGAGTTATTCCCACATAAAGATGGAATCGAGAAATCATCTCAGGTTATTCTAGATGCTGTATCATACCTGACTAAAACAGTGGACTCAAAGTCTCGTGATGTAAAAATTTTACAGCCAACAATTGCTACTCAGAGCTCTCGATTTTCGGAGGCTAACTCTCTATATGAGTTGGACAAAGCGACGCAAGAGGTGGTTAATGCTATTGTAGAAGCCCAATCTCTGGGAGGGCCTCTGAATAGTATCTCTCTGGGTCAGGAATTACCAACCATTGACATTTCAAGGTCGGTTGGACTACCGGAGCTTCGAAGACTACGGAGAACTTTCATCAAACTAACAGGGCAAACTAGTTTAAGCGGACGACCACCACCCTCTGATGCCGAGAGTGCCAAGAGGATGTTTGTTGACTACCTGAATAGGGAACTGGGGACTTCTTGAAAGTTGAAACTAGATTAACCTGAATGATAAGCTGTATACTTGTAGCATATGAACTACCAAACTTTACAAAGCAAGAGGTAACAGAGATATCACTCTTCTTTCTGGCAACTTTGCCATCAATTATTTAAGCTTTGTTGTTATCGATGTTTTTTCTCTTAGTAATCATAATATCTATGTCCTCGCTGTTACAGAACTATAGTCATATTCTGACATGAGATTTTCAGCTCATACGGTTCCTTGAGGGTCGTAAATAAATCTAAGGAGCACATCAGGATTATTTGTAGATTATGCTTTGGAAAATTACGGTCCTTATATATTCACACACACATGATATATACATATACATAGTTAATGAGTTAATTCAAAGAGAAAATGAGTGAATCTTTGGTTTCTTCTTTAAAACGAGTTCCAAGATTGTGTAATTGTTCTATTAATGAGTTCATACTTTGTTCATGCTTTTGATGTGAATGAGTTTCTTTCTTTGATAATGCATTTCATGGGTCTTTTAATGAGTTAATTTTATGGTTCGACTGCTGCTCAGACAACTCTACAAGATAGGGTATCTATCGAGACACTGGATAGGATCGGGGAAGGGAGGTATTATAGAAGTGCATTTAGAGTCAGAAGTACTTTCTTTATATGAGTAGGAATAGGTACACTGAGAATAGAGGAAGGGTATCTATCAAGGTCTTTCTTGATTCCGGAATTCTTAGAATAAGATCTCGAATGAGACAAAGCAATCACTCTTCCAGAGTATCTTAGATTAGGAGTCTTGCTTCCCGTATCATAATAGGAGCTGCTTGACCAATAGAGAATAGAGCCGGAAGTCTCTCTTTTTGTTTCATAGTCAGAAGTACTCTCTTTCTTGAGTTGCAACAATAGGAAGAGCTCCTCTCATTGTGCACATTGAATAAACCCTTCTCAGTTGTTTCAGAGTCAGACCTGGACAAGTTCTAGAGTAAGAAGAAGGGAGGGAGACATGATCGATTGGAGGAGAAAGAGTTAGAGGTTAGAGAAGTTTCTCTTTCATGAGTCACTGAACTAAATAGGGTTCGAATCCGAATTGCAAAGAAACCTGTGGGGTATGCTGCAAGACAAATATCTTATTTCGGACTTCTTATCATATCTAGCCTCTTATCTTCTGCTGCTGCACGTCTTGGGTTGAGTCGAGCAACTTTTCCCTTCATTAACTTCTTCCACGGAGAATGACATTGCACTTCTGTAAGAGTGAAAGAATAGTGCGAGCTTCCCCCTGATGTAGTCGAAAGATCTAATCCCACCGACCACCCTACAACGGGGGACCCGGACTATGAAGAAAGATCTCCTGCTTCCGCAACTTATGAAAAGAGGTCAAGCTCTTGGCTCCTTTCCATGAGAGAGATCAGATGTGCTCCGGTGGGGAAAGCTGAGTAGTTCAGTTGCCTTTGATTGTGAGTTCATTGGGAATCTTTGAAATCACCTCAAGGCTCAACCTATAGAGGAGAGGAACTTGGTTAGTTTATCTTTTCCAATACTCAAATAAATATTAGATAAATGTCTATTTTTATCTTGAATTTCAATTCTCATCAAATTATTGAAGTTAAGTGCCTGGAAAATATTTTGTTTTTATAAAAGATTATGTTTTACAAATATATTTTAAAAAGTGATTTTAAAAAAATAATAATAATAAAGAGCGCTTCATATACTCTTTATAGAAAAAACCACTAAAAACCAAATTTTATCACATTTATTTTTCTATGGAGTGTTTTAAGTTATAAAATATTAAGTATACAAAATTGGTAAATTTTCAAATAATGAAGACATTAGGGATTTTTCTTTTAATTAAAATTATATACTTTTTAATTGGAAAATTGTCAAAGAAAAAATTGAACAAAATACTTACCTTTCATAAAAAAAAATGAATTATTATTCTTTTAGTGGTTTTTTCTTTAGAAAAATGATTTTTTTTTTATTTTTTTTTGAAGTTTATAGTTTGTTTTTCTTTTTATTACTCTTGAAAGTAAACCTCTTTTTAATTTGAATATTAGTCAAAGTTGAATTTTTACAAAACTATTGTTGAAATTATTGAATTTAGTTTTACATTACGTTTGGTCAGACTATTGGATAGAACTTAGAAGGTGGAAATCGCAACATTTAATTTACCAATTGATGGAACAATGAAATTAATTTAAACGAATTGATATTCATATTAGATTACGGTATCTATCTCTCACAAATGTTAAAGAAAAAAAAATAACATATATACACACACATTGTAATTATCAAGATTTCATACTGAATCTGATTATTTTCTCTCTCAATATTTAAAGAATTAAAATTTTTAATTTAAATTAGTTAAAAAAAAAAAAATTCTCTTCTCCCATTTAAGAATATCAATGTCGAGATTTCAATTTTAAAGATACATTGATGATAGAATATTAATACCAAATTTATTTAGATTAATAAGTAACTTATTCTTACTCAAAAATAAGATTAAAAAATACTGTTATTAGTAGTCATATTCTTAATCCATGATTAACAACAATCCACGGATTGTTCTAACCCTATAATGAGCATTCATGTCCATCACATTGCGAATCATATTAAATGGAGGCAATGGGTTTTCTTGAGTCAGTCTCCTTGGATGATCAGAGAAAATTAATGGCAATTTTTCACAATTGAGCTCCATTCATTCAGTCTTTACATCTTGCAAGTCTTCAGCCGTCAGTAAAGCAAAAAGGAATAATTAATTTGAACAACGATGAATGAATCTGTGAATGTTTTGCTAATCAAGCTACGCACGGACAAAACTTGAGGGAGGAAAACTCAATTGAACGAAAAAATCAATCGGTGATGAAAGAAAATATGCTGCATTCAACATAGAAGAATGTACTGATAATTAAAAGATAACTATTTATTACCAAGTTGGTTTGTTGTTTAATTACATTACATAACTAATTTTCATTAGTAGAGACGAAATACTAACCATGAACATCTTCCAAATAAGTTGAACCCAACTTACAAGTTATGTTCTGGACTGCAATCCACCTTTTTGTTTGTTGGTTCAATCAAACATGATGTGAGGGGCTTACAACAAGTTTGAATGTCTTCCTCCATTCCCCACATAGGTATGACCTCTCCCTTTCTGTAATCAGGCAGAATCAGCAAATCAGTTTAATAAGATCTTTTTTAGGTGGTTTAGCCAACGAGTAAAGTTGTAAATTTTAGGATATTGTGAACTCATTAGAGCTGAGTTCACAGGGTAAAGAGGTAATAAAGCATAAAATTGATGTTTTTTAGTTTGGGACCCACAAACTCTTAGAGTCAATAAGGAGGTGAGTTCGCAAATTCCTCCCCTTCCAACTCGTCCTCCTCAAACCCCTTGAGCCAAACACCTTCATGACACTTCTAACTATAGTGTTCAGATAGAACAACAGAGACAACATAGAAAGCTTATCAAAGTAAATAATTTGCCTGAAATAAGTTACTGTTTTGAGAGCATTAACAAGTCATTTAGCCAAAGCAGTAATTGCTACGACCATCCTAGAGGTGAGAGGTCAGAATTCCCCAACATCCAATTTTTGTAAAAAGAAGAAACAAGCAGTTAGTTCTGAGTATAGGATCCTTACTGTGACAGATAACAGTTAACAACCGCTGATTTTTGCCACACGAAAGGTTCTAATAACCAACCTTCACTTTTCCAACAACATCCTAACATTTCAACCATTTTTGAGATGTATCTTCCTTTCCACCTAGTTACTGCTTGGCTGCTAAGTGAAGTCACTCTCGAACTTCTTTCCAGTTTCTAAAGTTATTAAACAATTATACGTTTTATAATAGACATGCAAGAGAAAATATCCATTTTTTTTCCTCGTGTTGTTAATTTCAGTATCTCAAAATGCCTATCA

Coding sequence (CDS)

ATGGCGTTGGTTGTGATCTGTGGCCAGCCATGCAGTGGGAAGTCCACTGCTGCACTCTGCTTAGCTGAAGCTCTCAAGGAATCAAATGTAAAAGAAGCCATTAAGATTATCGATGAAACTTCTCTTCATTTGGATCGGAATCAGAGCTATGCCAACATGCCTGCTGAAAAAAATCTAAGAGGAGTTCTCAGATCTGAAGTTGATAGATCTGTATCTAAAGATAGCATTGTCATTGTAGACTCTTTGAACAGTATAAAGGGTTATCGATATGAACTGTGGTGTTTGGCTCGTGGAACAGGAATCAGGTATTGTGTGCTGTACTGCGACGTGGAAGAAACCAATTGTAGGAAGTGGAATGAAGAGCGTCGTGAGAAAGAAGAAGCGAGCTATGATGATAAGATATTCGAAGATCTTCTCAGAAGGTTTGAGAGGCCAAATAGTAGAAATAGATGGGACTCACCATTGTTTGAGTTATTCCCACATAAAGATGGAATCGAGAAATCATCTCAGGTTATTCTAGATGCTGTATCATACCTGACTAAAACAGTGGACTCAAAGTCTCGTGATGTAAAAATTTTACAGCCAACAATTGCTACTCAGAGCTCTCGATTTTCGGAGGCTAACTCTCTATATGAGTTGGACAAAGCGACGCAAGAGGTGGTTAATGCTATTGTAGAAGCCCAATCTCTGGGAGGGCCTCTGAATAGTATCTCTCTGGGTCAGGAATTACCAACCATTGACATTTCAAGGTCGGTTGGACTACCGGAGCTTCGAAGACTACGGAGAACTTTCATCAAACTAACAGGGCAAACTAGTTTAAGCGGACGACCACCACCCTCTGATGCCGAGAGTGCCAAGAGGATGTTTGTTGACTACCTGAATAGGGAACTGGGGACTTCTTGA

Protein sequence

MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLRGVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNEERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLTKTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLGQELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS
Homology
BLAST of Cucsat.G4274 vs. ExPASy Swiss-Prot
Match: Q9LMH0 (Protein KTI12 homolog OS=Arabidopsis thaliana OX=3702 GN=KTI12 PE=1 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.0e-125
Identity = 219/298 (73.49%), Postives = 261/298 (87.58%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKS AA+ LAE LKES  K++++IIDE S HLDRNQ+YANMPAEKNLR
Sbjct: 1   MALVVICGQPCSGKSIAAVTLAETLKESETKQSVRIIDEASFHLDRNQNYANMPAEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           G LRS+VDRSVS   IVIVDSLNSIKGYRYELWC+AR  GIRYCV+YCDV+E +CR+WN+
Sbjct: 61  GKLRSDVDRSVSTGEIVIVDSLNSIKGYRYELWCIARAAGIRYCVVYCDVDEAHCRQWNK 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ER ++ E  YDD IFEDL+RRFE+P  RNRWDSPLFEL+P ++ I+KSS VIL+AV+YLT
Sbjct: 121 ERSDRGEDGYDDGIFEDLVRRFEKPERRNRWDSPLFELYPSREVIDKSSPVILEAVTYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
           KTVDSK++DV+ILQP+IATQ++RFSEANSLYELD+ATQE++NAIVE QSLG  ++ ++LG
Sbjct: 181 KTVDSKTQDVRILQPSIATQAARFSEANSLYELDRATQEIINAIVEQQSLGAAISRVTLG 240

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELG 299
            ELP I+I R +GLPELRRLRRTF+KL GQ+SLSG P P+DA+SAKR FVDYLNRE G
Sbjct: 241 NELPPIEICRPIGLPELRRLRRTFVKLMGQSSLSGPPLPTDADSAKRRFVDYLNREFG 298

BLAST of Cucsat.G4274 vs. ExPASy Swiss-Prot
Match: B8BK80 (Protein KTI12 homolog OS=Oryza sativa subsp. indica OX=39946 GN=OsI_35919 PE=3 SV=2)

HSP 1 Score: 437.2 bits (1123), Expect = 1.5e-121
Identity = 219/301 (72.76%), Postives = 257/301 (85.38%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKS AA CLA AL  S     ++IIDE+SLHL RN SY +M  EKNLR
Sbjct: 1   MALVVICGQPCSGKSAAAACLAAALCSSTSDLTVRIIDESSLHLGRNDSYKDMVVEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVS+DSI++VDSLN+IKGYRYELWCLAR +GIRYCVL+CD E  +CR+WN 
Sbjct: 61  GVLRSEVDRSVSRDSIIVVDSLNNIKGYRYELWCLARASGIRYCVLFCDTEVDHCREWNT 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           +R+EK E +YD+ IF+DL+ RFE+P+ RNRWDSPLFELFP +DG+ +SS VI +AVSYLT
Sbjct: 121 KRQEKGEPTYDNNIFDDLVSRFEKPDRRNRWDSPLFELFPSRDGVMESSPVIAEAVSYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQS--LGGPLNSIS 240
           K VDSK+RDVK+LQPTIATQ++R +EANSLYE+DKATQEV+NAIVEAQS  LG P+N IS
Sbjct: 181 KKVDSKTRDVKVLQPTIATQTARTTEANSLYEMDKATQEVINAIVEAQSCGLGLPVNKIS 240

Query: 241 LGQELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELG 300
           LG +LPTI + RSVGLPELR LRRTFIKL GQ SLSG PPP+DA+SA RMFVDYLNRE+ 
Sbjct: 241 LGPDLPTICLQRSVGLPELRSLRRTFIKLAGQYSLSGPPPPADADSATRMFVDYLNREIS 300

BLAST of Cucsat.G4274 vs. ExPASy Swiss-Prot
Match: B9GAG9 (Protein KTI12 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=KTI12 PE=3 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 1.5e-121
Identity = 219/301 (72.76%), Postives = 257/301 (85.38%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKS AA CLA AL  S     ++IIDE+SLHL RN SY +M  EKNLR
Sbjct: 1   MALVVICGQPCSGKSAAAACLAAALCSSTSDLTVRIIDESSLHLGRNDSYKDMVVEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVS+DSI++VDSLN+IKGYRYELWCLAR +GIRYCVL+CD E  +CR+WN 
Sbjct: 61  GVLRSEVDRSVSRDSIIVVDSLNNIKGYRYELWCLARASGIRYCVLFCDTEVDHCREWNT 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           +R+EK E +YD+ IF+DL+ RFE+P+ RNRWDSPLFELFP +DG+ +SS VI +AVSYLT
Sbjct: 121 KRQEKGEPTYDNNIFDDLVSRFEKPDRRNRWDSPLFELFPSRDGVMESSPVIAEAVSYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQS--LGGPLNSIS 240
           K VDSK+RDVK+LQPTIATQ++R +EANSLYE+DKATQEV+NAIVEAQS  LG P+N IS
Sbjct: 181 KKVDSKTRDVKVLQPTIATQTARTTEANSLYEMDKATQEVINAIVEAQSCGLGLPVNKIS 240

Query: 241 LGQELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELG 300
           LG +LPTI + RSVGLPELR LRRTFIKL GQ SLSG PPP+DA+SA RMFVDYLNRE+ 
Sbjct: 241 LGPDLPTICLQRSVGLPELRSLRRTFIKLAGQYSLSGPPPPADADSATRMFVDYLNREIS 300

BLAST of Cucsat.G4274 vs. ExPASy Swiss-Prot
Match: Q0P457 (Protein KTI12 homolog OS=Danio rerio OX=7955 GN=kti12 PE=2 SV=2)

HSP 1 Score: 189.1 bits (479), Expect = 7.2e-47
Identity = 114/297 (38.38%), Postives = 170/297 (57.24%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           M L+++CG PCSGK+  A  L +   + N    + I+ +    +D+N  YA+   EKNLR
Sbjct: 1   MPLILMCGYPCSGKTRRAQELRDYFTQ-NTGRKVHIVGDEDQGIDKNSVYADSQKEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           G LR+EV+R V+KD IVI+DSLN IKGYRYEL+CL + T   +C++Y         +WN+
Sbjct: 61  GALRAEVERKVNKDDIVILDSLNYIKGYRYELFCLIKHTQTPHCLVYSLTSADLSSEWNK 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           +R  + ++ Y  +I + L+ RFE P+SRNRWDSPLF        I++   +  +A+    
Sbjct: 121 DR--EADSQYTQEILDALILRFEAPDSRNRWDSPLFT-------IQQDDSLPFEAI---- 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
              D+  +  K   P  +T+S   S  N LYELDK TQ+V+ A++E+Q    P + IS+ 
Sbjct: 181 --CDALFKR-KAPPPNQSTKSQPLSSTNFLYELDKVTQDVLMAVLESQKTSVPGDLISIP 240

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNREL 298
                I+++RS+ + ELR+LRR FI  T         P  +      MFV YLN+ +
Sbjct: 241 GATEKIELTRSLNMVELRKLRRQFISYTKM------HPTENIGQIANMFVQYLNKSM 274

BLAST of Cucsat.G4274 vs. ExPASy Swiss-Prot
Match: Q4KLF3 (Protein KTI12 homolog OS=Xenopus laevis OX=8355 GN=kti12 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.6e-46
Identity = 116/297 (39.06%), Postives = 171/297 (57.58%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           M L+++CG PCSGKS+ +  L E L++S  K  + II +  L +DRN  YA+   EK+LR
Sbjct: 1   MPLILLCGFPCSGKSSRSQELQEHLEQSGRK--VHIIGDHVLGVDRNAVYADSREEKDLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           G LR+ V+R ++K+ +VI+DSLN IKGYRYEL+CL +     +C+++C         WN+
Sbjct: 61  GSLRAAVERKLNKEDVVILDSLNYIKGYRYELFCLIKHVQTPHCLIHCITSPEVSSTWNQ 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
            R + E+  Y+ +IF+ L++RFE P+SRNRWDSPLF +  HKD  +   + I +A+ +  
Sbjct: 121 HRDKNEQ--YNQEIFDSLVQRFEFPDSRNRWDSPLFSV--HKDE-KLPLEQICNAIFHR- 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
                     K   P  +TQ    S  N L+ELDK TQEVV A++ AQ    P + I + 
Sbjct: 181 ----------KAPPPNQSTQMQPLSSTNFLHELDKVTQEVVTAVLSAQKTSIPGDVIMVP 240

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNREL 298
                + + R + + ELRRLR+ FI      S +   P  +      MFV YLN+ +
Sbjct: 241 GASEKVQLPRILSMSELRRLRQQFI------SYTKLHPNENISQLANMFVQYLNQSI 273

BLAST of Cucsat.G4274 vs. NCBI nr
Match: XP_031744146.1 (protein KTI12 homolog isoform X2 [Cucumis sativus] >XP_031744147.1 protein KTI12 homolog isoform X2 [Cucumis sativus] >XP_031744149.1 protein KTI12 homolog isoform X2 [Cucumis sativus] >XP_031744150.1 protein KTI12 homolog isoform X2 [Cucumis sativus])

HSP 1 Score: 590 bits (1521), Expect = 1.99e-212
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR
Sbjct: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE
Sbjct: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT
Sbjct: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
           KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG
Sbjct: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300
           QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS
Sbjct: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300

BLAST of Cucsat.G4274 vs. NCBI nr
Match: XP_031744145.1 (protein KTI12 homolog isoform X1 [Cucumis sativus] >XP_031744148.1 protein KTI12 homolog isoform X1 [Cucumis sativus] >KAE8647243.1 hypothetical protein Csa_019032 [Cucumis sativus] >KAE8647249.1 hypothetical protein Csa_018281 [Cucumis sativus])

HSP 1 Score: 590 bits (1521), Expect = 2.55e-211
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR
Sbjct: 70  MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 129

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE
Sbjct: 130 GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 189

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT
Sbjct: 190 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 249

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
           KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG
Sbjct: 250 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 309

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300
           QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS
Sbjct: 310 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 369

BLAST of Cucsat.G4274 vs. NCBI nr
Match: KAA0064758.1 (protein KTI12-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 578 bits (1489), Expect = 1.50e-207
Identity = 291/300 (97.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKSTAALCLAEALKESNVK+AIKIIDETSLHLDRN+SYANMPAEKNLR
Sbjct: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKQAIKIIDETSLHLDRNKSYANMPAEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEET+CRKWNE
Sbjct: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETDCRKWNE 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ERREKEE+SYDDKIFEDL+RRFERP+SRNRWDSPLFELFPHKDGI+KS+QVILDAVSYLT
Sbjct: 121 ERREKEESSYDDKIFEDLVRRFERPDSRNRWDSPLFELFPHKDGIDKSAQVILDAVSYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
           KTVDSKSRDVKILQPTIATQ+SRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG
Sbjct: 181 KTVDSKSRDVKILQPTIATQNSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300
           QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS
Sbjct: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300

BLAST of Cucsat.G4274 vs. NCBI nr
Match: XP_016899904.1 (PREDICTED: protein KTI12 homolog [Cucumis melo])

HSP 1 Score: 577 bits (1487), Expect = 3.03e-207
Identity = 292/300 (97.33%), Postives = 299/300 (99.67%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKSTAALCLAEALKESNVK+AIKIIDETSLHLDRN+SYANMPAEKNLR
Sbjct: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKQAIKIIDETSLHLDRNKSYANMPAEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEET+CRKWNE
Sbjct: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETDCRKWNE 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ERREKEEASYDDKIFEDL+RRFERP+SRNRWDSPLFEL PHKDGI+KSSQVILDAVSYLT
Sbjct: 121 ERREKEEASYDDKIFEDLVRRFERPDSRNRWDSPLFELSPHKDGIDKSSQVILDAVSYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
           KTVDSKSRDVKILQPTIATQ+SRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG
Sbjct: 181 KTVDSKSRDVKILQPTIATQNSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300
           QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS
Sbjct: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300

BLAST of Cucsat.G4274 vs. NCBI nr
Match: XP_038886675.1 (protein KTI12 homolog [Benincasa hispida] >XP_038886676.1 protein KTI12 homolog [Benincasa hispida] >XP_038886677.1 protein KTI12 homolog [Benincasa hispida])

HSP 1 Score: 563 bits (1452), Expect = 6.56e-202
Identity = 283/300 (94.33%), Postives = 294/300 (98.00%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKSTAALCLAE LKESNVK+A++IIDETSLHLDRNQSYANMPAEKNLR
Sbjct: 1   MALVVICGQPCSGKSTAALCLAEVLKESNVKQAVRIIDETSLHLDRNQSYANMPAEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE
Sbjct: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
            RREK EA+YDDKIFEDL+RRFERP+SRNRWDSPLF LFPHKDGIEKSSQVILDAVSYLT
Sbjct: 121 VRREKGEANYDDKIFEDLVRRFERPDSRNRWDSPLFALFPHKDGIEKSSQVILDAVSYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
           KTVDSKSRDVKILQPTIATQ+SRFSEANSLYELDKATQEVVNAIVEAQ+LGGPLNSI LG
Sbjct: 181 KTVDSKSRDVKILQPTIATQNSRFSEANSLYELDKATQEVVNAIVEAQALGGPLNSIPLG 240

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300
           QELPTI+ISRSVGLPELRRLRRTFIKLTGQTSLSG PPPSDAESAKRMF+DYLNRELGT+
Sbjct: 241 QELPTINISRSVGLPELRRLRRTFIKLTGQTSLSGPPPPSDAESAKRMFIDYLNRELGTA 300

BLAST of Cucsat.G4274 vs. ExPASy TrEMBL
Match: A0A5A7VAY4 (Protein KTI12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00630 PE=4 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 7.28e-208
Identity = 291/300 (97.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKSTAALCLAEALKESNVK+AIKIIDETSLHLDRN+SYANMPAEKNLR
Sbjct: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKQAIKIIDETSLHLDRNKSYANMPAEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEET+CRKWNE
Sbjct: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETDCRKWNE 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ERREKEE+SYDDKIFEDL+RRFERP+SRNRWDSPLFELFPHKDGI+KS+QVILDAVSYLT
Sbjct: 121 ERREKEESSYDDKIFEDLVRRFERPDSRNRWDSPLFELFPHKDGIDKSAQVILDAVSYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
           KTVDSKSRDVKILQPTIATQ+SRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG
Sbjct: 181 KTVDSKSRDVKILQPTIATQNSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300
           QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS
Sbjct: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300

BLAST of Cucsat.G4274 vs. ExPASy TrEMBL
Match: A0A1S4DV92 (protein KTI12 homolog OS=Cucumis melo OX=3656 GN=LOC107990703 PE=4 SV=1)

HSP 1 Score: 577 bits (1487), Expect = 1.47e-207
Identity = 292/300 (97.33%), Postives = 299/300 (99.67%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKSTAALCLAEALKESNVK+AIKIIDETSLHLDRN+SYANMPAEKNLR
Sbjct: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKQAIKIIDETSLHLDRNKSYANMPAEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEET+CRKWNE
Sbjct: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETDCRKWNE 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ERREKEEASYDDKIFEDL+RRFERP+SRNRWDSPLFEL PHKDGI+KSSQVILDAVSYLT
Sbjct: 121 ERREKEEASYDDKIFEDLVRRFERPDSRNRWDSPLFELSPHKDGIDKSSQVILDAVSYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
           KTVDSKSRDVKILQPTIATQ+SRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG
Sbjct: 181 KTVDSKSRDVKILQPTIATQNSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300
           QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS
Sbjct: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELGTS 300

BLAST of Cucsat.G4274 vs. ExPASy TrEMBL
Match: A0A6J1BRL5 (protein KTI12 homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005080 PE=4 SV=1)

HSP 1 Score: 542 bits (1397), Expect = 8.24e-194
Identity = 274/302 (90.73%), Postives = 288/302 (95.36%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKSTAALCLAEALKESN K+AI+IIDE S HLDRNQSYANMPAEKNLR
Sbjct: 1   MALVVICGQPCSGKSTAALCLAEALKESNAKQAIRIIDEASFHLDRNQSYANMPAEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVS+D+IVIVDSLNSIKGYRYELWCLARG GIRYCVLYCDVEET+CRKWNE
Sbjct: 61  GVLRSEVDRSVSRDNIVIVDSLNSIKGYRYELWCLARGAGIRYCVLYCDVEETHCRKWNE 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ERREK EA+YDDKIFEDL+RRFERP+SRNRWDSPLFEL PHKDGIEKSS +ILDAVSYLT
Sbjct: 121 ERREKGEANYDDKIFEDLVRRFERPDSRNRWDSPLFELLPHKDGIEKSSTIILDAVSYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQS--LGGPLNSIS 240
           K VDSKSRDVKILQPTIATQ+SRFSEANSLYELDKATQEVVNAIVEAQ+  +GGPLN IS
Sbjct: 181 KAVDSKSRDVKILQPTIATQNSRFSEANSLYELDKATQEVVNAIVEAQAQAMGGPLNGIS 240

Query: 241 LGQELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELG 300
           LGQELP I+ISRSVGLPELRRLRRTFIKLTGQTSLSG PPPSDAESAKRMFVDYLNREL 
Sbjct: 241 LGQELPAINISRSVGLPELRRLRRTFIKLTGQTSLSGPPPPSDAESAKRMFVDYLNRELE 300

BLAST of Cucsat.G4274 vs. ExPASy TrEMBL
Match: A0A6J1GJU3 (protein KTI12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111454536 PE=4 SV=1)

HSP 1 Score: 528 bits (1359), Expect = 5.09e-188
Identity = 265/302 (87.75%), Postives = 285/302 (94.37%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVV+CGQPCSGKSTAALCLAEALKESNVK+A+KII+ETS HLDRNQSYA+MPAEKNLR
Sbjct: 1   MALVVVCGQPCSGKSTAALCLAEALKESNVKQAVKIINETSFHLDRNQSYADMPAEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVS+D+IVIVDSLNSIKGYRYELWCLARG GIRYCVLYCDVEET+CRKWN+
Sbjct: 61  GVLRSEVDRSVSEDNIVIVDSLNSIKGYRYELWCLARGAGIRYCVLYCDVEETHCRKWND 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ERRE  EA+YDDKIFEDL+RRFERP SRNRWDSPLFEL PHKDGIEKSS VILDAVSYLT
Sbjct: 121 ERRENGEANYDDKIFEDLVRRFERPISRNRWDSPLFELLPHKDGIEKSSTVILDAVSYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQ--SLGGPLNSIS 240
           K VDSKSRDVKILQPTIAT+S+R SEANSLYELDKAT EVVNAIVEAQ  ++GGPLN + 
Sbjct: 181 KEVDSKSRDVKILQPTIATRSTRLSEANSLYELDKATHEVVNAIVEAQGQAMGGPLNGVP 240

Query: 241 LGQELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELG 300
           LGQ+LPTI+IS+SVGLPELRRLRRTFIKLTGQTSLSG PPP DA SAKRMFVDYLNRELG
Sbjct: 241 LGQDLPTINISKSVGLPELRRLRRTFIKLTGQTSLSGPPPPCDAASAKRMFVDYLNRELG 300

BLAST of Cucsat.G4274 vs. ExPASy TrEMBL
Match: A0A6J1HBW2 (protein KTI12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462670 PE=4 SV=1)

HSP 1 Score: 525 bits (1352), Expect = 8.24e-187
Identity = 265/302 (87.75%), Postives = 287/302 (95.03%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKSTAALCLAEALKESNVK+AI+IIDETS HLDRNQSYANMPAEKNLR
Sbjct: 10  MALVVICGQPCSGKSTAALCLAEALKESNVKQAIRIIDETSFHLDRNQSYANMPAEKNLR 69

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           GVLRSEVDRSVS+DSIVIVDSLNSIKGYRYELWCLARG GIRYCVLYC VEET+CRKWNE
Sbjct: 70  GVLRSEVDRSVSRDSIVIVDSLNSIKGYRYELWCLARGAGIRYCVLYCGVEETHCRKWNE 129

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ERREK EA+YDDKIFEDL+RRFERP+S+NRWD PLFEL PH+DGIE  S V+ DAV +LT
Sbjct: 130 ERREKAEANYDDKIFEDLVRRFERPDSKNRWDVPLFELRPHEDGIETRSAVLGDAVLFLT 189

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQS--LGGPLNSIS 240
           KTV+S+SRDVKILQPTIATQS+RFSEANSLYELDKATQEVV+AIVEAQ+  +GGPLN IS
Sbjct: 190 KTVNSRSRDVKILQPTIATQSNRFSEANSLYELDKATQEVVSAIVEAQAQAMGGPLNGIS 249

Query: 241 LGQELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELG 300
           LGQELPTI+ISRSVGLPEL+RLRRTFIKLTGQTSLSG PPPSDAESAKRMF+DYL+RELG
Sbjct: 250 LGQELPTINISRSVGLPELQRLRRTFIKLTGQTSLSGPPPPSDAESAKRMFIDYLHRELG 309

BLAST of Cucsat.G4274 vs. TAIR 10
Match: AT1G13870.1 (calmodulin binding;purine nucleotide binding )

HSP 1 Score: 451.1 bits (1159), Expect = 7.2e-127
Identity = 219/298 (73.49%), Postives = 261/298 (87.58%), Query Frame = 0

Query: 1   MALVVICGQPCSGKSTAALCLAEALKESNVKEAIKIIDETSLHLDRNQSYANMPAEKNLR 60
           MALVVICGQPCSGKS AA+ LAE LKES  K++++IIDE S HLDRNQ+YANMPAEKNLR
Sbjct: 1   MALVVICGQPCSGKSIAAVTLAETLKESETKQSVRIIDEASFHLDRNQNYANMPAEKNLR 60

Query: 61  GVLRSEVDRSVSKDSIVIVDSLNSIKGYRYELWCLARGTGIRYCVLYCDVEETNCRKWNE 120
           G LRS+VDRSVS   IVIVDSLNSIKGYRYELWC+AR  GIRYCV+YCDV+E +CR+WN+
Sbjct: 61  GKLRSDVDRSVSTGEIVIVDSLNSIKGYRYELWCIARAAGIRYCVVYCDVDEAHCRQWNK 120

Query: 121 ERREKEEASYDDKIFEDLLRRFERPNSRNRWDSPLFELFPHKDGIEKSSQVILDAVSYLT 180
           ER ++ E  YDD IFEDL+RRFE+P  RNRWDSPLFEL+P ++ I+KSS VIL+AV+YLT
Sbjct: 121 ERSDRGEDGYDDGIFEDLVRRFEKPERRNRWDSPLFELYPSREVIDKSSPVILEAVTYLT 180

Query: 181 KTVDSKSRDVKILQPTIATQSSRFSEANSLYELDKATQEVVNAIVEAQSLGGPLNSISLG 240
           KTVDSK++DV+ILQP+IATQ++RFSEANSLYELD+ATQE++NAIVE QSLG  ++ ++LG
Sbjct: 181 KTVDSKTQDVRILQPSIATQAARFSEANSLYELDRATQEIINAIVEQQSLGAAISRVTLG 240

Query: 241 QELPTIDISRSVGLPELRRLRRTFIKLTGQTSLSGRPPPSDAESAKRMFVDYLNRELG 299
            ELP I+I R +GLPELRRLRRTF+KL GQ+SLSG P P+DA+SAKR FVDYLNRE G
Sbjct: 241 NELPPIEICRPIGLPELRRLRRTFVKLMGQSSLSGPPLPTDADSAKRRFVDYLNREFG 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMH01.0e-12573.49Protein KTI12 homolog OS=Arabidopsis thaliana OX=3702 GN=KTI12 PE=1 SV=1[more]
B8BK801.5e-12172.76Protein KTI12 homolog OS=Oryza sativa subsp. indica OX=39946 GN=OsI_35919 PE=3 S... [more]
B9GAG91.5e-12172.76Protein KTI12 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=KTI12 PE=3 SV=... [more]
Q0P4577.2e-4738.38Protein KTI12 homolog OS=Danio rerio OX=7955 GN=kti12 PE=2 SV=2[more]
Q4KLF33.6e-4639.06Protein KTI12 homolog OS=Xenopus laevis OX=8355 GN=kti12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_031744146.11.99e-212100.00protein KTI12 homolog isoform X2 [Cucumis sativus] >XP_031744147.1 protein KTI12... [more]
XP_031744145.12.55e-211100.00protein KTI12 homolog isoform X1 [Cucumis sativus] >XP_031744148.1 protein KTI12... [more]
KAA0064758.11.50e-20797.00protein KTI12-like protein [Cucumis melo var. makuwa][more]
XP_016899904.13.03e-20797.33PREDICTED: protein KTI12 homolog [Cucumis melo][more]
XP_038886675.16.56e-20294.33protein KTI12 homolog [Benincasa hispida] >XP_038886676.1 protein KTI12 homolog ... [more]
Match NameE-valueIdentityDescription
A0A5A7VAY47.28e-20897.00Protein KTI12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S4DV921.47e-20797.33protein KTI12 homolog OS=Cucumis melo OX=3656 GN=LOC107990703 PE=4 SV=1[more]
A0A6J1BRL58.24e-19490.73protein KTI12 homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005080 ... [more]
A0A6J1GJU35.09e-18887.75protein KTI12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111454536 PE=4 SV=1[more]
A0A6J1HBW28.24e-18787.75protein KTI12 homolog OS=Cucurbita moschata OX=3662 GN=LOC111462670 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G13870.17.2e-12773.49calmodulin binding;purine nucleotide binding [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 108..128
NoneNo IPR availablePANTHERPTHR12435:SF4BNAC08G40070D PROTEINcoord: 1..298
IPR013641Protein KTI12/L-seryl-tRNA(Sec) kinasePFAMPF08433KTI12coord: 1..294
e-value: 2.2E-83
score: 279.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 1..186
e-value: 2.0E-36
score: 127.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 3..148
IPR045106Protein Kti12PANTHERPTHR12435UNCHARACTERIZEDcoord: 1..298

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G4274.T6Cucsat.G4274.T6mRNA
Cucsat.G4274.T1Cucsat.G4274.T1mRNA
Cucsat.G4274.T4Cucsat.G4274.T4mRNA
Cucsat.G4274.T9Cucsat.G4274.T9mRNA
Cucsat.G4274.T3Cucsat.G4274.T3mRNA
Cucsat.G4274.T8Cucsat.G4274.T8mRNA
Cucsat.G4274.T5Cucsat.G4274.T5mRNA
Cucsat.G4274.T7Cucsat.G4274.T7mRNA
Cucsat.G4274.T2Cucsat.G4274.T2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0002098 tRNA wobble uridine modification
molecular_function GO:0005524 ATP binding