Cucsat.G401 (gene) Cucumber (B10) v3

Overview


Name	Cucsat.G401
Type	gene
Organism	Cucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
Description	Chaperone protein dnaJ 20
Location	ctg1: 8454933 .. 8456562 (-)
RNA-Seq Expression	Cucsat.G401
Synteny	Cucsat.G401

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCAGCACCGTACAAATGACTCAGCGTTGGACGTCTCGTATAAATAGCGGAATATGATAGAACGGAAGATAGACGGAGAAAAACAGAGAGAAATGCAGTGTTCCGATTTAACCTTTTCAGGGAGCGATTCACGCTTCTACATCCCTTCTAACCCTACCACTACCGCCCGCCGACCAATTTCCGGCTACCGATCTCGGGTTTGCTTTCCACACACTCCGCCGTTCAATTCGACGCGCTTGCCGTCTCTTTCAATTCGAGCCAAGGCCTCGTTCAACGAAGGATTCGTTTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGCATATCTAAGTCCGGATCCTTGGAGGAAATCAAGCGCGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTTTCACCGCCGGGGTGTGTGGAGGAAAATACGAAGAGGTTCATTCGTGTTCAGGAGGCGTACGAAACGTTAGCAGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGATTGGAGGGCTTCAAGTCGCGTTCTCTGCTAGGAGACGATACGACGCCGATGAGGTATTATATTAGTTCACTCTACCGATTTCAACTTTCTTTTTTCGGTATTTTAAGGATAATCATGTTTAATTCTTAGCGGATTTTGGAACCTCCTTCGATTTTTTTGTGTTTCCCCTTGTTTTTTTAAAGTGATTTCGTGTTTAATTTCTCATATGGTGTCCATAGAGATTAGAATCAGTGAAGAATTTTAATCTCAATGATTTTGAATTTTTAAAGTGAGGAAAGGAAGCGTCGTTTTTCTTTTCCTCTTTCCTTTTTGTTCTTCCGACTGTCTTATACGGACGCAGTACGATTGGTAGTGTATACTGTTCATGTTTAAACAAGGACCTATCGTTGATTATTCAGTGATTTGTTCTATGATCAAGAAACGGAATTGCACTAGCAAATCCTCCCAATATGGAATTCCGTTACTGTAAACCTCTAATTTTCCCTACTCTTCTGTTTACATTCAACATTTTCACCTAATTTCTTTCCTTCTCCATTAAATTCTTGTGTTCTACCGAACCTGTAAGGAATCCTTTATTTTTCTTCCTCCACTGAGTCTAACAAACAATCAATCCTATTGCAAAATTTCGATTTCCTATTTTCATATCATATACTCAACCTTCCAAATTTGATATTGGATTCAGGAAGTTGCACAGAAAAGTGGATGGAGAAACAGTTGGGAAGCTCAGATTTCGGAATTGAAGAGAAGAAGTATGGAGAAGGATTTGAGACCAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAACGAACAAAGTTGATGGAAATAGAATTATTCATATCAAGGAAAACCCATGTTTGACTCAGAATCAGTAAATAGAATACGGCTTATTCGGTAACATTAGCTTTTGTTGTTAGTTTTTTCTTTGTAATTTCCTTGAAACCTACTCTATGTGAAAAGCTTAACAATAAAAAGAAAAAGTGAGTCATTTTCAAATGACAAATAATTATATGCATTTCTACCCCAAATGCTCATTTTGATGCCCAAACCCGAGTATTACCCTTTGAAATATAGCAAAATATGAACAAAAGTATAGCAAAATTGTTGATTAACT

Coding sequence (CDS)

ATGATAGAACGGAAGATAGACGGAGAAAAACAGAGAGAAATGCAGTGTTCCGATTTAACCTTTTCAGGGAGCGATTCACGCTTCTACATCCCTTCTAACCCTACCACTACCGCCCGCCGACCAATTTCCGGCTACCGATCTCGGGTTTGCTTTCCACACACTCCGCCGTTCAATTCGACGCGCTTGCCGTCTCTTTCAATTCGAGCCAAGGCCTCGTTCAACGAAGGATTCGTTTCCTCCGAGGTCGCCGAAGGCTCCTTCTACGATTTGCTCGGCATATCTAAGTCCGGATCCTTGGAGGAAATCAAGCGCGCGTACAAGCAGCTCGCTCGGAAGTACCATCCGGACGTTTCACCGCCGGGGTGTGTGGAGGAAAATACGAAGAGGTTCATTCGTGTTCAGGAGGCGTACGAAACGTTAGCAGATCCGAGGAGGAGAGCGCTGTATGATAGAGATATGATTGGAGGGCTTCAAGTCGCGTTCTCTGCTAGGAGACGATACGACGCCGATGAGGAAGTTGCACAGAAAAGTGGATGGAGAAACAGTTGGGAAGCTCAGATTTCGGAATTGAAGAGAAGAAGTATGGAGAAGGATTTGAGACCAAATATGTCATGGGGAGCTCGAATGCGCCGGCAAATGAACGAACAAAGTTGA

Protein sequence

MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS

Homology

[Close]

BLAST of Cucsat.G401 vs. ExPASy Swiss-Prot
Match: Q9SDN0 (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 164.9 bits (416), Expect = 1.0e-39
Identity = 97/191 (50.79%), Postives = 128/191 (67.02%), Query Frame = 0


Query: 33  NPTTTARRPISGYR-----SRVCFPHTPPFNSTRLPSLSIRAKASFNEGFVSSEVAEGSF 92
           +P    ++PIS  +     + + +P    F+STR     I+++ + ++    SE  + SF
Sbjct: 15  HPFFYKQQPISSLQPTSIPTTISYPTRTRFSSTR-----IQSRLTHDDPVKQSE--DLSF 74

Query: 93  YDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLADPRRRA 152
           YDLLG+++S +L EIK+AYKQLARKYHPDVSPP  VEE T RFIRVQEAYETL+DPRRR 
Sbjct: 75  YDLLGVTESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRV 134

Query: 153 LYDRDMIGGLQVAFSARRRYDADEEVA-QKSGWRNSWEAQISELKRRSMEKDLRPNMSWG 212
           LYDRD+  G   +FS RR+   D+EV  +KS W+  W+ Q+S L+RRS +KD    MSW 
Sbjct: 135 LYDRDLSMGFSFSFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKD-NNTMSWA 194

Query: 213 ARMRRQMNEQS 218
           ARMRRQ  E S
Sbjct: 195 ARMRRQQQESS 197

[Close]

BLAST of Cucsat.G401 vs. ExPASy Swiss-Prot
Match: A5VJE8 (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.4e-13
Identity = 39/79 (49.37%), Postives = 55/79 (69.62%), Query Frame = 0


Query: 82  VAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLA 141
           +AE  +YD+LG+SK  S ++IKRAY++LA KYHPDV+     EE   +F ++ EAYETL+
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 142 DPRRRALYDRDMIGGLQVA 161
           D ++RA YD+    G Q A
Sbjct: 61  DSQKRAQYDQFGSAGPQGA 76

[Close]

BLAST of Cucsat.G401 vs. ExPASy Swiss-Prot
Match: B2G6W4 (Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) OX=557433 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.4e-13
Identity = 39/79 (49.37%), Postives = 55/79 (69.62%), Query Frame = 0


Query: 82  VAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLA 141
           +AE  +YD+LG+SK  S ++IKRAY++LA KYHPDV+     EE   +F ++ EAYETL+
Sbjct: 1   MAEQDYYDILGVSKDASEKDIKRAYRRLAAKYHPDVNHEPGAEE---KFKKINEAYETLS 60

Query: 142 DPRRRALYDRDMIGGLQVA 161
           D ++RA YD+    G Q A
Sbjct: 61  DSQKRAQYDQFGSAGPQGA 76

[Close]

BLAST of Cucsat.G401 vs. ExPASy Swiss-Prot
Match: Q9Z9E9 (Chaperone protein DnaJ OS=Chlamydia pneumoniae OX=83558 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 8.4e-13
Identity = 37/65 (56.92%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 87  FYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLADPRRR 146
           +Y +LGISK+ S EEIK+AY++LA KYHPD +P     E  KRF  V EAYE L+DP++R
Sbjct: 3   YYSILGISKTASAEEIKKAYRKLAVKYHPDKNPGDAAAE--KRFKEVSEAYEVLSDPQKR 62

Query: 147 ALYDR 152
             YDR
Sbjct: 63  DSYDR 65

[Close]

BLAST of Cucsat.G401 vs. ExPASy Swiss-Prot
Match: A7GT07 (Chaperone protein DnaJ OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98) OX=315749 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 37/71 (52.11%), Postives = 55/71 (77.46%), Query Frame = 0


Query: 82  VAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEEN-TKRFIRVQEAYETL 141
           +++  +Y++LG+SKS S +EIK+AY++LA+KYHPDVS     EEN  ++F  VQEAYE L
Sbjct: 1   MSKRDYYEVLGVSKSASKDEIKKAYRRLAKKYHPDVSK----EENAVEKFKEVQEAYEVL 60

Query: 142 ADPRRRALYDR 152
           +D ++RA YD+
Sbjct: 61  SDEQKRAQYDQ 67

[Close]

BLAST of Cucsat.G401 vs. NCBI nr
Match: XP_004133982.1 (chaperone protein dnaJ 20, chloroplastic [Cucumis sativus] >KGN56713.1 hypothetical protein Csa_009538 [Cucumis sativus])

HSP 1 Score: 434 bits (1117), Expect = 1.28e-153
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST 60
           MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST
Sbjct: 1   MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST 60

Query: 61  RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP 120
           RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP
Sbjct: 61  RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP 120

Query: 121 GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR 180
           GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR
Sbjct: 121 GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR 180

Query: 181 NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS 217
           NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS
Sbjct: 181 NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS 217

[Close]

BLAST of Cucsat.G401 vs. NCBI nr
Match: XP_008438327.1 (PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo])

HSP 1 Score: 396 bits (1018), Expect = 2.53e-138
Identity = 200/215 (93.02%), Postives = 205/215 (95.35%), Query Frame = 0


Query: 2   IERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTR 61
           IER+IDGEKQREMQCSD TFSGSDSRFYIPSNPT +ARRPISGYRSRV FPHTPPFNSTR
Sbjct: 15  IERRIDGEKQREMQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTR 74

Query: 62  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPG 121
           LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPG
Sbjct: 75  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPG 134

Query: 122 CVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRN 181
             EE TK FIRVQEAYETL+DPRRRALYDRDMIGGLQVAFSARRRYDA EEVA+KSGWRN
Sbjct: 135 GAEEYTKMFIRVQEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRN 194

Query: 182 SWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQ 216
           SWEAQISELKRRSMEKDLR NMSWGARMRRQMNEQ
Sbjct: 195 SWEAQISELKRRSMEKDLRQNMSWGARMRRQMNEQ 229

[Close]

BLAST of Cucsat.G401 vs. NCBI nr
Match: XP_038893222.1 (chaperone protein dnaJ 20, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 335 bits (859), Expect = 1.69e-114
Identity = 170/203 (83.74%), Postives = 178/203 (87.68%), Query Frame = 0


Query: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73
           MQCSD TFSGSDSRFYIPSNP  T RRPI  +RSRV FP TPP NSTR P LSIRAKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSNPNITGRRPIPCHRSRVFFPSTPPCNSTRSPPLSIRAKASF 60

Query: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133
           NEG VSSEVAEGSFYDLLGIS+SGSLEEIK AYKQLARKYHPDVSPPG  EE TKRFIRV
Sbjct: 61  NEGTVSSEVAEGSFYDLLGISQSGSLEEIKHAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120

Query: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193
           QEAYETL+DPRRRALYDRDM+GGLQVAFS RRRYDA EEV  +  WRN W+AQISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSGRRRYDAHEEVPGRREWRNGWQAQISELKRR 180

Query: 194 SMEKDLRPNMSWGARMRRQMNEQ 216
           SM++D R NMSWGARMRRQMNEQ
Sbjct: 181 SMDRDSRQNMSWGARMRRQMNEQ 203

[Close]

BLAST of Cucsat.G401 vs. NCBI nr
Match: KAG7018873.1 (Chaperone protein dnaJ 20, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 323 bits (829), Expect = 6.29e-110
Identity = 162/203 (79.80%), Postives = 178/203 (87.68%), Query Frame = 0


Query: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73
           MQCSD TFSGSDSRFYIPS+P+ T RRPI+G+RS + FP+T P N  RLPSLSIRAKASF
Sbjct: 1   MQCSDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60

Query: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133
           NEG VSSEVA+GSFYDLLG+S+S SL EIKRAYK LARKYHPDVSP GC EE TKRFIRV
Sbjct: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRV 120

Query: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193
           QEAYETL+DPRRRALYDRDM+GGLQVAFSA R YDAD+E  +KSGWR+ W++QISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180

Query: 194 SMEKDLRPNMSWGARMRRQMNEQ 216
           SM KD   NMSWGARMRRQMNEQ
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQ 203

[Close]

BLAST of Cucsat.G401 vs. NCBI nr
Match: XP_022949677.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 322 bits (826), Expect = 5.87e-109
Identity = 164/215 (76.28%), Postives = 182/215 (84.65%), Query Frame = 0


Query: 2   IERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTR 61
           +ER+    K REMQCSD T+S  D R  IPS+P+ T RR ISG RSRV FP+TPP NSTR
Sbjct: 26  VERR---RKNREMQCSDFTYSVGDYRILIPSSPSITGRRQISGQRSRVFFPNTPPCNSTR 85

Query: 62  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPG 121
           LPSLSIRAKASFN G  SSE A+GSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPG
Sbjct: 86  LPSLSIRAKASFNGGIASSEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPG 145

Query: 122 CVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRN 181
             EE TKRFIRVQEAYETL+DPRRRALYDRDM+GGLQVAFSARRRYD DE V  KS W++
Sbjct: 146 RAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKS 205

Query: 182 SWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQ 216
            W+AQISELK+RSM+KD + N+SWGARMR+QMNEQ
Sbjct: 206 CWKAQISELKKRSMDKDSKHNLSWGARMRQQMNEQ 237

[Close]

BLAST of Cucsat.G401 vs. ExPASy TrEMBL
Match: A0A0A0L4L9 (Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus OX=3659 GN=Csa_3G129700 PE=4 SV=1)

HSP 1 Score: 434 bits (1117), Expect = 6.19e-154
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST 60
           MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST
Sbjct: 1   MIERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNST 60

Query: 61  RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP 120
           RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP
Sbjct: 61  RLPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPP 120

Query: 121 GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR 180
           GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR
Sbjct: 121 GCVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWR 180

Query: 181 NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS 217
           NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS
Sbjct: 181 NSWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQS 217

[Close]

BLAST of Cucsat.G401 vs. ExPASy TrEMBL
Match: A0A1S3AWS2 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103483465 PE=4 SV=1)

HSP 1 Score: 396 bits (1018), Expect = 1.22e-138
Identity = 200/215 (93.02%), Postives = 205/215 (95.35%), Query Frame = 0


Query: 2   IERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTR 61
           IER+IDGEKQREMQCSD TFSGSDSRFYIPSNPT +ARRPISGYRSRV FPHTPPFNSTR
Sbjct: 15  IERRIDGEKQREMQCSDFTFSGSDSRFYIPSNPTISARRPISGYRSRVFFPHTPPFNSTR 74

Query: 62  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPG 121
           LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPG
Sbjct: 75  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISQSGSLAEIKRAYKQLARKYHPDVSPPG 134

Query: 122 CVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRN 181
             EE TK FIRVQEAYETL+DPRRRALYDRDMIGGLQVAFSARRRYDA EEVA+KSGWRN
Sbjct: 135 GAEEYTKMFIRVQEAYETLSDPRRRALYDRDMIGGLQVAFSARRRYDAAEEVAEKSGWRN 194

Query: 182 SWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQ 216
           SWEAQISELKRRSMEKDLR NMSWGARMRRQMNEQ
Sbjct: 195 SWEAQISELKRRSMEKDLRQNMSWGARMRRQMNEQ 229

[Close]

BLAST of Cucsat.G401 vs. ExPASy TrEMBL
Match: A0A6J1GCT3 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452997 PE=4 SV=1)

HSP 1 Score: 322 bits (826), Expect = 2.84e-109
Identity = 164/215 (76.28%), Postives = 182/215 (84.65%), Query Frame = 0


Query: 2   IERKIDGEKQREMQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTR 61
           +ER+    K REMQCSD T+S  D R  IPS+P+ T RR ISG RSRV FP+TPP NSTR
Sbjct: 26  VERR---RKNREMQCSDFTYSVGDYRILIPSSPSITGRRQISGQRSRVFFPNTPPCNSTR 85

Query: 62  LPSLSIRAKASFNEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPG 121
           LPSLSIRAKASFN G  SSE A+GSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPG
Sbjct: 86  LPSLSIRAKASFNGGIASSEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPG 145

Query: 122 CVEENTKRFIRVQEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRN 181
             EE TKRFIRVQEAYETL+DPRRRALYDRDM+GGLQVAFSARRRYD DE V  KS W++
Sbjct: 146 RAEEYTKRFIRVQEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKS 205

Query: 182 SWEAQISELKRRSMEKDLRPNMSWGARMRRQMNEQ 216
            W+AQISELK+RSM+KD + N+SWGARMR+QMNEQ
Sbjct: 206 CWKAQISELKKRSMDKDSKHNLSWGARMRQQMNEQ 237

[Close]

BLAST of Cucsat.G401 vs. ExPASy TrEMBL
Match: A0A6J1E8M4 (chaperone protein dnaJ 20, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431786 PE=4 SV=1)

HSP 1 Score: 320 bits (819), Expect = 1.01e-108
Identity = 160/203 (78.82%), Postives = 176/203 (86.70%), Query Frame = 0


Query: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73
           MQC D TFSGSDSRFYIPS+P+ T RRPI+G+RS + FP+T P N  RLPSLSIRAKASF
Sbjct: 1   MQCPDFTFSGSDSRFYIPSSPSITGRRPITGHRSGIFFPNTLPCNLMRLPSLSIRAKASF 60

Query: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133
           NEG VSSEVA+GSFYDLLG+S+S SL EIKRAYK LARKYHPDVSP GC EE TKRFIR 
Sbjct: 61  NEGIVSSEVADGSFYDLLGVSESVSLAEIKRAYKHLARKYHPDVSPIGCAEEYTKRFIRF 120

Query: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193
           QEAYETL+DPRRRALYDRDM+GGLQVAFSA R YDAD+E  +KSGWR+ W++QISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSAWRLYDADQEAPEKSGWRSCWQSQISELKRR 180

Query: 194 SMEKDLRPNMSWGARMRRQMNEQ 216
           SM KD   NMSWGARMRRQMNEQ
Sbjct: 181 SMNKDSSQNMSWGARMRRQMNEQ 203

[Close]

BLAST of Cucsat.G401 vs. ExPASy TrEMBL
Match: A0A6J1IB32 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111473471 PE=4 SV=1)

HSP 1 Score: 317 bits (812), Expect = 1.18e-107
Identity = 159/203 (78.33%), Postives = 175/203 (86.21%), Query Frame = 0


Query: 14  MQCSDLTFSGSDSRFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSIRAKASF 73
           MQCSD T+SG D R  IPS+P+ T RR ISG +SRV FP+TPP NSTRLPSLSIRAKASF
Sbjct: 1   MQCSDFTYSGGDYRILIPSSPSITGRRQISGQKSRVFFPNTPPCNSTRLPSLSIRAKASF 60

Query: 74  NEGFVSSEVAEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRV 133
           N G  S E A+GSFYDLLGIS+SGSL EIKRAYKQLARKYHPDVSPPG  EE TKRFIRV
Sbjct: 61  NGGIASFEAADGSFYDLLGISESGSLAEIKRAYKQLARKYHPDVSPPGRAEEYTKRFIRV 120

Query: 134 QEAYETLADPRRRALYDRDMIGGLQVAFSARRRYDADEEVAQKSGWRNSWEAQISELKRR 193
           QEAYETL+DPRRRALYDRDM+GGLQVAFSARRRYD DE V  KS W++ W+AQISELKRR
Sbjct: 121 QEAYETLSDPRRRALYDRDMVGGLQVAFSARRRYDVDEGVPDKSAWKSCWKAQISELKRR 180

Query: 194 SMEKDLRPNMSWGARMRRQMNEQ 216
           SM+KD + N+SWGARMR+QMNEQ
Sbjct: 181 SMDKDSKHNLSWGARMRQQMNEQ 203

[Close]

BLAST of Cucsat.G401 vs. TAIR 10
Match: AT4G13830.2 (DNAJ-like 20 )

HSP 1 Score: 164.9 bits (416), Expect = 7.4e-41
Identity = 97/191 (50.79%), Postives = 128/191 (67.02%), Query Frame = 0


Query: 33  NPTTTARRPISGYR-----SRVCFPHTPPFNSTRLPSLSIRAKASFNEGFVSSEVAEGSF 92
           +P    ++PIS  +     + + +P    F+STR     I+++ + ++    SE  + SF
Sbjct: 15  HPFFYKQQPISSLQPTSIPTTISYPTRTRFSSTR-----IQSRLTHDDPVKQSE--DLSF 74

Query: 93  YDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLADPRRRA 152
           YDLLG+++S +L EIK+AYKQLARKYHPDVSPP  VEE T RFIRVQEAYETL+DPRRR 
Sbjct: 75  YDLLGVTESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRV 134

Query: 153 LYDRDMIGGLQVAFSARRRYDADEEVA-QKSGWRNSWEAQISELKRRSMEKDLRPNMSWG 212
           LYDRD+  G   +FS RR+   D+EV  +KS W+  W+ Q+S L+RRS +KD    MSW 
Sbjct: 135 LYDRDLSMGFSFSFSGRRQNRYDQEVVEEKSEWKAKWQTQLSGLRRRSNQKD-NNTMSWA 194

Query: 213 ARMRRQMNEQS 218
           ARMRRQ  E S
Sbjct: 195 ARMRRQQQESS 197

[Close]

BLAST of Cucsat.G401 vs. TAIR 10
Match: AT4G13830.1 (DNAJ-like 20 )

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-29
Identity = 74/143 (51.75%), Postives = 97/143 (67.83%), Query Frame = 0


Query: 33  NPTTTARRPISGYR-----SRVCFPHTPPFNSTRLPSLSIRAKASFNEGFVSSEVAEGSF 92
           +P    ++PIS  +     + + +P    F+STR     I+++ + ++    SE  + SF
Sbjct: 15  HPFFYKQQPISSLQPTSIPTTISYPTRTRFSSTR-----IQSRLTHDDPVKQSE--DLSF 74

Query: 93  YDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLADPRRRA 152
           YDLLG+++S +L EIK+AYKQLARKYHPDVSPP  VEE T RFIRVQEAYETL+DPRRR 
Sbjct: 75  YDLLGVTESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEAYETLSDPRRRV 134

Query: 153 LYDRDMIGGLQVAFSARR--RYD 169
           LYDRD+  G   +FS RR  RYD
Sbjct: 135 LYDRDLSMGFSFSFSGRRQNRYD 150

[Close]

BLAST of Cucsat.G401 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 69.3 bits (168), Expect = 4.3e-12
Identity = 47/138 (34.06%), Postives = 71/138 (51.45%), Query Frame = 0


Query: 27  RFYIPSNPTTTARRPISGYRSRVCFPHTPPFNSTRLPSLSI----RAKASFNEGFVSSEV 86
           R Y PS   +T  +  S   + +    +  F+   LP LS+    R       G   +  
Sbjct: 23  RAYYPSRIESTRHQNSSSQVNCLGASKSSMFSHGSLPFLSMTGMSRNMHPPRRGSRFTVR 82

Query: 87  AEGSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVSPPGCVEENTKRFIRVQEAYETLAD 146
           A+  +Y +LG+SK+ +  EIK AY++LAR YHPDV+     EE   +F  +  AYE L+D
Sbjct: 83  ADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAYEVLSD 142

Query: 147 PRRRALYDRDMIGGLQVA 161
             +++LYDR    GL+ A
Sbjct: 143 DEKKSLYDRYGEAGLKGA 157

[Close]

BLAST of Cucsat.G401 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 68.6 bits (166), Expect = 7.3e-12
Identity = 34/68 (50.00%), Postives = 49/68 (72.06%), Query Frame = 0


Query: 85  GSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVS-PPGCVEENTKRFIRVQEAYETLADP 144
           G +Y  LG+SKS + +EIK AY++LAR+YHPDV+  PG     T++F  +  AYE L+D 
Sbjct: 74  GDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEPGA----TEKFKEISAAYEVLSDE 133

Query: 145 RRRALYDR 152
           ++RALYD+
Sbjct: 134 QKRALYDQ 137

[Close]

BLAST of Cucsat.G401 vs. TAIR 10
Match: AT1G80030.3 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 68.6 bits (166), Expect = 7.3e-12
Identity = 34/68 (50.00%), Postives = 49/68 (72.06%), Query Frame = 0


Query: 85  GSFYDLLGISKSGSLEEIKRAYKQLARKYHPDVS-PPGCVEENTKRFIRVQEAYETLADP 144
           G +Y  LG+SKS + +EIK AY++LAR+YHPDV+  PG     T++F  +  AYE L+D 
Sbjct: 74  GDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKEPGA----TEKFKEISAAYEVLSDE 133

Query: 145 RRRALYDR 152
           ++RALYD+
Sbjct: 134 QKRALYDQ 137

The following BLAST results are available for this feature:

BLAST of Cucsat.G401 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SDN0	1.0e-39	50.79	Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2...	[more]
A5VJE8	6.4e-13	49.37	Chaperone protein DnaJ OS=Lactobacillus reuteri (strain DSM 20016) OX=557436 GN=...	[more]
B2G6W4	6.4e-13	49.37	Chaperone protein DnaJ OS=Lactobacillus reuteri (strain JCM 1112) OX=557433 GN=d...	[more]
Q9Z9E9	8.4e-13	56.92	Chaperone protein DnaJ OS=Chlamydia pneumoniae OX=83558 GN=dnaJ PE=3 SV=1	[more]
A7GT07	1.1e-12	52.11	Chaperone protein DnaJ OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / ...	[more]

BLAST of Cucsat.G401 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004133982.1	1.28e-153	100.00	chaperone protein dnaJ 20, chloroplastic [Cucumis sativus] >KGN56713.1 hypotheti...	[more]
XP_008438327.1	2.53e-138	93.02	PREDICTED: chaperone protein dnaJ 20, chloroplastic-like [Cucumis melo]	[more]
XP_038893222.1	1.69e-114	83.74	chaperone protein dnaJ 20, chloroplastic-like [Benincasa hispida]	[more]
KAG7018873.1	6.29e-110	79.80	Chaperone protein dnaJ 20, chloroplastic [Cucurbita argyrosperma subsp. argyrosp...	[more]
XP_022949677.1	5.87e-109	76.28	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of Cucsat.G401 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4L9	6.19e-154	100.00	Chaperone protein dnaJ 20, chloroplast OS=Cucumis sativus OX=3659 GN=Csa_3G12970...	[more]
A0A1S3AWS2	1.22e-138	93.02	chaperone protein dnaJ 20, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1GCT3	2.84e-109	76.28	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1E8M4	1.01e-108	78.82	chaperone protein dnaJ 20, chloroplastic-like isoform X1 OS=Cucurbita moschata O...	[more]
A0A6J1IB32	1.18e-107	78.33	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]

BLAST of Cucsat.G401 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13830.2	7.4e-41	50.79	DNAJ-like 20	[more]
AT4G13830.1	1.3e-29	51.75	DNAJ-like 20	[more]
AT2G22360.1	4.3e-12	34.06	DNAJ heat shock family protein	[more]
AT1G80030.1	7.3e-12	50.00	Molecular chaperone Hsp40/DnaJ family protein	[more]
AT1G80030.3	7.3e-12	50.00	Molecular chaperone Hsp40/DnaJ family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 106..121 score: 48.68 coord: 125..145 score: 35.96 coord: 145..164 score: 23.64 coord: 88..106 score: 48.04
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 85..145 e-value: 2.3E-20 score: 83.7
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 87..150 e-value: 1.9E-20 score: 72.7
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 86..153 score: 20.357307
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 87..142 e-value: 1.66936E-19 score: 76.8153
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 77..188 e-value: 3.2E-22 score: 80.6
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 86..160
None	No IPR available	PANTHER	PTHR45090	CHAPERONE PROTEIN DNAJ 20 CHLOROPLASTIC	coord: 14..217
None	No IPR available	PANTHER	PTHR45090:SF4	CHAPERONE PROTEIN DNAJ 20, CHLOROPLASTIC	coord: 14..217
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 130..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsat.G401.T1	Cucsat.G401.T1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
cellular_component	GO:0009507	chloroplast