Cucsat.G3662 (gene) Cucumber (B10) v3

BLAST of Cucsat.G3662 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 9.8e-282
Identity = 470/589 (79.80%), Postives = 541/589 (91.85%), Query Frame = 0

Query: 4   GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLA 63
           GYEATNGKMNETTE+RSPRDSDGHGTHTASI+AGRYVFPASTLGYA G AAGMAPKARLA
Sbjct: 187 GYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLA 246

Query: 64  AYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVFV 123
           AYKVCWN+GCYDSDILAAFD AV+DGVDV+SLSVGGVVVPYYLDAIAIGA+ A+  G+FV
Sbjct: 247 AYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFV 306

Query: 124 SASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPGR 183
           SASAGNGGPG LTVTNVAPW+TTVGAGT+DRDFPA+VKLGNG+++ G SVYGGP L PGR
Sbjct: 307 SASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGR 366

Query: 184 LYPLIYAGT-EGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 243
           +YPL+Y G+  GGDGYSSSLCLEGSL+PNLVKGKIVLCDRGINSRA KGE+V+K GGLGM
Sbjct: 367 MYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGM 426

Query: 244 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSH--LQPTATILFKGTRLG 303
           I+ANGVFDGEGLVADCHVLPAT+VGASGGDEIR+YI+E++KS     PTATI+FKGTRLG
Sbjct: 427 IIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLG 486

Query: 304 VRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILS 363
           +RPAPVVASFSARGPNPE+PEI+KPDVIAPGLNILAAWPD+IGPSG+ +D R TEFNILS
Sbjct: 487 IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILS 546

Query: 364 GTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDF 423
           GTSMACPHVSGLAALLKAAHP WSPAAI+SAL+TTAYT+DN GE M+DES+GNTS+V+D+
Sbjct: 547 GTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDY 606

Query: 424 GAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNL 483
           G+GHVHP KAMDPGL+YD+ +YDY++FLCNSNYT  NI  IT + ADC GA+RAGH GNL
Sbjct: 607 GSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNL 666

Query: 484 NYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVG 543
           NYPS +VVFQQYG+ KMSTHFIRTVTNVGD++S+Y++ I+PP G +VTVEPEKL+FRRVG
Sbjct: 667 NYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVG 726

Query: 544 QKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPL 590
           QKLSF+VRV+   V+LSPG++++++G I+W+DGK  VTSPLVVT+QQPL
Sbjct: 727 QKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of Cucsat.G3662 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 3.3e-181
Identity = 329/588 (55.95%), Postives = 426/588 (72.45%), Query Frame = 0

Query: 7   ATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLAAYK 66
           A  G +N+T E+ SPRD+DGHGTHT+S AAGR+ F AS  GYA G A G+APKAR+AAYK
Sbjct: 185 AVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYK 244

Query: 67  VCW-NAGCYDSDILAAFDAAVSDGVDVVSLSVG---GVVVPYYLDAIAIGAYRAVAAGVF 126
           VCW ++GC DSDILAAFDAAV DGVDV+S+S+G   G+  PYYLD IAIG+Y A + G+F
Sbjct: 245 VCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIF 304

Query: 127 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 186
           VS+SAGN GP G++VTN+APWVTTVGA T+DR+FPAD  LG+G  + G S+Y G  L  G
Sbjct: 305 VSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPL-NG 364

Query: 187 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 246
           R++P++Y G  G    S+SLC+E +L+P  V+GKIV+CDRG + R AKG VVKKAGG+GM
Sbjct: 365 RMFPVVYPGKSGMS--SASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGM 424

Query: 247 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 306
           ILANG  +GEGLV D H++PA AVG++ GD I+ Y    A SH  P A+I F+GT +G++
Sbjct: 425 ILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAY----ASSHPNPIASIDFRGTIVGIK 484

Query: 307 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 366
           PAPV+ASFS RGPN  SPEI+KPD+IAPG+NILAAW D +GP+G+P+D R TEFNILSGT
Sbjct: 485 PAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGT 544

Query: 367 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 426
           SMACPHVSG AALLK+AHP WSPA I+SA+MTT   +DN   +++DES+G ++T  D+G+
Sbjct: 545 SMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGS 604

Query: 427 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 486
           GH++  +AM+PGL+YD+   DY+ FLC+  Y  K IQVIT     C   ++    GNLNY
Sbjct: 605 GHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPS-PGNLNY 664

Query: 487 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 546
           PS+  VF    +  +S   IRT TNVG A ++Y+  I+ P G++VTV+P +L F    ++
Sbjct: 665 PSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKR 724

Query: 547 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTD-GKHEVTSPLVVTMQQPL 590
            S+ V V      +  G +    GS+ W D GKH V SP+VVT    L
Sbjct: 725 RSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of Cucsat.G3662 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 3.7e-164
Identity = 311/581 (53.53%), Postives = 398/581 (68.50%), Query Frame = 0

Query: 4   GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLA 63
           GYE+T G ++E+ E RSPRD DGHGTHT+S AAG  V  AS LGYA G A GMAP+AR+A
Sbjct: 189 GYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVA 248

Query: 64  AYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVFV 123
            YKVCW  GC+ SDILAA D A++D V+V+S+S+GG +  YY D +AIGA+ A+  G+ V
Sbjct: 249 VYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILV 308

Query: 124 SASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPGR 183
           S SAGN GP   +++NVAPW+TTVGAGT+DRDFPA   LGNG+   G S++ G AL P +
Sbjct: 309 SCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL-PDK 368

Query: 184 LYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGMI 243
           L P IYAG    +  + +LC+ G+L P  VKGKIV+CDRGIN+R  KG+VVK AGG+GMI
Sbjct: 369 LLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMI 428

Query: 244 LANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVRP 303
           LAN   +GE LVAD H+LPAT VG   GD IR Y+     +   PTA+I   GT +GV+P
Sbjct: 429 LANTAANGEELVADAHLLPATTVGEKAGDIIRHYVT----TDPNPTASISILGTVVGVKP 488

Query: 304 APVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGTS 363
           +PVVA+FS+RGPN  +P I+KPD+IAPG+NILAAW    GP+G+ +D R  EFNI+SGTS
Sbjct: 489 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTS 548

Query: 364 MACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGAG 423
           M+CPHVSGLAALLK+ HP WSPAAI+SALMTTAY     G+ +LD ++G  ST  D GAG
Sbjct: 549 MSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAG 608

Query: 424 HVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNYP 483
           HV P  A +PGLIYDL T DY+ FLC  NYT+  I+ ++ +   C  +K +    +LNYP
Sbjct: 609 HVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSK-SYSVADLNYP 668

Query: 484 SLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQKL 543
           S AV     G +K    + RTVT+VG A +         +G+ ++VEP  L F+   +K 
Sbjct: 669 SFAVNVDGVGAYK----YTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKK 728

Query: 544 SFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVT 585
           S+ V      V  S  S S   GSI W+DGKH V SP+ ++
Sbjct: 729 SYTV---TFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Cucsat.G3662 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 4.1e-163
Identity = 309/576 (53.65%), Postives = 399/576 (69.27%), Query Frame = 0

Query: 7   ATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLAAYK 66
           A+ G  +   E  SPRD DGHGTHT++ AAG  V  AS LGYA G A GMA +AR+A YK
Sbjct: 188 ASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYK 247

Query: 67  VCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVFVSAS 126
           VCW+ GC+ SDILAA D A+ DGVDV+SLS+GG   PYY D IAIGA+ A+  GVFVS S
Sbjct: 248 VCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCS 307

Query: 127 AGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPGRLYP 186
           AGN GP   +V NVAPWV TVGAGT+DRDFPA   LGNG+ + G S+Y G  +    L  
Sbjct: 308 AGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPL-E 367

Query: 187 LIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGMILAN 246
           L+Y     G+  SS+LCL GSL+ ++V+GKIV+CDRG+N+R  KG VV+ AGGLGMI+AN
Sbjct: 368 LVY---NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMAN 427

Query: 247 GVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVRPAPV 306
               GE LVAD H+LPA AVG   GD +R+Y+    KS  +PTA ++FKGT L V+P+PV
Sbjct: 428 TAASGEELVADSHLLPAIAVGKKTGDLLREYV----KSDSKPTALLVFKGTVLDVKPSPV 487

Query: 307 VASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGTSMAC 366
           VA+FS+RGPN  +PEI+KPDVI PG+NILA W D IGP+G+  D R T+FNI+SGTSM+C
Sbjct: 488 VAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSC 547

Query: 367 PHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGAGHVH 426
           PH+SGLA LLKAAHP WSP+AIKSALMTTAY LDN    + D +  + S     G+GHV 
Sbjct: 548 PHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVD 607

Query: 427 PQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNYPSLA 486
           PQKA+ PGL+YD++T +Y+ FLC+ +YT  +I  I  K    + +K+    G LNYPS +
Sbjct: 608 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIV-KRPSVNCSKKFSDPGQLNYPSFS 667

Query: 487 VVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQKLSFL 546
           V+F      K    + R VTNVG A+S+YKVT+     + ++V+P KL+F+ VG+K  + 
Sbjct: 668 VLF----GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 727

Query: 547 VR-VQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPL 582
           V  V    V +   ++  + GSI W++ +HEV SP+
Sbjct: 728 VTFVSKKGVSM---TNKAEFGSITWSNPQHEVRSPV 747

BLAST of Cucsat.G3662 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 3.4e-157
Identity = 293/587 (49.91%), Postives = 391/587 (66.61%), Query Frame = 0

Query: 4   GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLA 63
           GYEA  GK++E  EY+SPRD DGHGTHTA+  AG  V  A+  G+A G A GMA KAR+A
Sbjct: 204 GYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVA 263

Query: 64  AYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVFV 123
           AYKVCW  GC+ SDIL+A D AV+DGV V+S+S+GG V  Y  D+++I  + A+  GVFV
Sbjct: 264 AYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFV 323

Query: 124 SASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIP-G 183
           S SAGNGGP  +++TNV+PW+TTVGA TMDRDFPA VK+G  R   G S+Y G  ++P  
Sbjct: 324 SCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKN 383

Query: 184 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 243
           + YPL+Y G        +S CL+G+L+   V GKIV+CDRG+  R  KG+VVK+AGG+GM
Sbjct: 384 KQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGM 443

Query: 244 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 303
           +L N   +GE LVAD H+LPA AVG   G  I++Y   + K+    TA++   GTR+G++
Sbjct: 444 VLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKA----TASLEILGTRIGIK 503

Query: 304 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 363
           P+PVVA+FS+RGPN  S EI+KPD++APG+NILAAW   + PS + +D R  +FNILSGT
Sbjct: 504 PSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGT 563

Query: 364 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 423
           SM+CPHVSG+AAL+K+ HP WSPAAIKSALMTTAY  DN  + + D S    S+  D GA
Sbjct: 564 SMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGA 623

Query: 424 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 483
           GH+ P +A DPGL+YD+   +Y +FLC  + +   ++V T           A + GNLNY
Sbjct: 624 GHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNY 683

Query: 484 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 543
           P+++ +F +   H  +    RTVTNVG   S YKV++ P  G SVTV+P+ L F    QK
Sbjct: 684 PAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQK 743

Query: 544 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPL 590
           LS+ V  +       P     + G ++W    H+V SP+++T   PL
Sbjct: 744 LSYTVTFRTRFRMKRP-----EFGGLVWKSTTHKVRSPVIITWLPPL 780

BLAST of Cucsat.G3662 vs. NCBI nr
Match: XP_004141727.1 (subtilisin-like protease SBT1.5 [Cucumis sativus] >KGN45447.1 hypothetical protein Csa_015997 [Cucumis sativus])

HSP 1 Score: 1155 bits (2987), Expect = 0.0
Identity = 587/588 (99.83%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 3   SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 62
           SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL
Sbjct: 190 SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 249

Query: 63  AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 122
           AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF
Sbjct: 250 AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 309

Query: 123 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 182
           VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG
Sbjct: 310 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 369

Query: 183 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 242
           RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM
Sbjct: 370 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 429

Query: 243 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 302
           ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR
Sbjct: 430 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 489

Query: 303 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 362
           PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT
Sbjct: 490 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 549

Query: 363 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 422
           SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA
Sbjct: 550 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 609

Query: 423 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 482
           GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGH+GNLNY
Sbjct: 610 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHSGNLNY 669

Query: 483 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 542
           PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK
Sbjct: 670 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 729

Query: 543 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 590
           LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ
Sbjct: 730 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 777

BLAST of Cucsat.G3662 vs. NCBI nr
Match: KAA0059362.1 (subtilisin-like protease SBT1.5 [Cucumis melo var. makuwa] >TYK03964.1 subtilisin-like protease SBT1.5 [Cucumis melo var. makuwa])

HSP 1 Score: 1149 bits (2973), Expect = 0.0
Identity = 584/588 (99.32%), Postives = 586/588 (99.66%), Query Frame = 0

Query: 3   SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 62
           SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL
Sbjct: 190 SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 249

Query: 63  AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 122
           AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF
Sbjct: 250 AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 309

Query: 123 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 182
           VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG
Sbjct: 310 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 369

Query: 183 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 242
           RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM
Sbjct: 370 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 429

Query: 243 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 302
           ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSH  PTATILFKGTRLGVR
Sbjct: 430 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHSPPTATILFKGTRLGVR 489

Query: 303 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 362
           PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT
Sbjct: 490 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 549

Query: 363 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 422
           SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA
Sbjct: 550 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 609

Query: 423 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 482
           GHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQVITGKIADCSGAKRAGHTGNLNY
Sbjct: 610 GHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQVITGKIADCSGAKRAGHTGNLNY 669

Query: 483 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 542
           PSL+VVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK
Sbjct: 670 PSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 729

Query: 543 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 590
           LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ
Sbjct: 730 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 777

BLAST of Cucsat.G3662 vs. NCBI nr
Match: XP_008462247.1 (PREDICTED: subtilisin-like protease SBT1.5 [Cucumis melo])

HSP 1 Score: 1148 bits (2969), Expect = 0.0
Identity = 583/588 (99.15%), Postives = 585/588 (99.49%), Query Frame = 0

Query: 3   SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 62
           SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL
Sbjct: 190 SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 249

Query: 63  AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 122
           AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF
Sbjct: 250 AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 309

Query: 123 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 182
           VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG
Sbjct: 310 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 369

Query: 183 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 242
           RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM
Sbjct: 370 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 429

Query: 243 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 302
           ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSH  PTATILFKGTRLGVR
Sbjct: 430 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHSPPTATILFKGTRLGVR 489

Query: 303 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 362
           PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT
Sbjct: 490 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 549

Query: 363 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 422
           SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA
Sbjct: 550 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 609

Query: 423 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 482
           GHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQVITGKIADCSGAKRAGHTGNLNY
Sbjct: 610 GHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQVITGKIADCSGAKRAGHTGNLNY 669

Query: 483 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 542
           PSL+VVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK
Sbjct: 670 PSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 729

Query: 543 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 590
           LSFLVRVQAMAVRLSPGSSSMK GSIIWTDGKHEVTSPLVVTMQQPLQ
Sbjct: 730 LSFLVRVQAMAVRLSPGSSSMKGGSIIWTDGKHEVTSPLVVTMQQPLQ 777

BLAST of Cucsat.G3662 vs. NCBI nr
Match: XP_038897862.1 (subtilisin-like protease SBT1.5 [Benincasa hispida])

HSP 1 Score: 1139 bits (2945), Expect = 0.0
Identity = 575/588 (97.79%), Postives = 585/588 (99.49%), Query Frame = 0

Query: 3   SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 62
           SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL
Sbjct: 201 SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 260

Query: 63  AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 122
           AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA+RAVA GVF
Sbjct: 261 AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAFRAVAGGVF 320

Query: 123 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 182
           VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPAL+PG
Sbjct: 321 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALVPG 380

Query: 183 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 242
           RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKK+GGLGM
Sbjct: 381 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKSGGLGM 440

Query: 243 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 302
           ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSH  PTATILFKGTRLGVR
Sbjct: 441 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHSPPTATILFKGTRLGVR 500

Query: 303 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 362
           PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWP+KIGPSGIPTDKRTTEFNILSGT
Sbjct: 501 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPEKIGPSGIPTDKRTTEFNILSGT 560

Query: 363 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 422
           SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA
Sbjct: 561 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 620

Query: 423 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 482
           GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNY+TKNIQVITGKIADCSGAKRAGHTGNLNY
Sbjct: 621 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYSTKNIQVITGKIADCSGAKRAGHTGNLNY 680

Query: 483 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 542
           PSL+VVFQQYGKHKMSTHFIRTVTNVGD+NSIYKV+IKPPSGISVTVEPEKLAFRRVGQK
Sbjct: 681 PSLSVVFQQYGKHKMSTHFIRTVTNVGDSNSIYKVSIKPPSGISVTVEPEKLAFRRVGQK 740

Query: 543 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 590
           L+FLVRVQAMAVRLSPGSSSMKSGSI+WTDGKHEVTSPLVVTMQQPLQ
Sbjct: 741 LNFLVRVQAMAVRLSPGSSSMKSGSIVWTDGKHEVTSPLVVTMQQPLQ 788

BLAST of Cucsat.G3662 vs. NCBI nr
Match: XP_022992123.1 (subtilisin-like protease SBT1.5 [Cucurbita maxima])

HSP 1 Score: 1128 bits (2917), Expect = 0.0
Identity = 570/588 (96.94%), Postives = 582/588 (98.98%), Query Frame = 0

Query: 3   SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 62
           SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL
Sbjct: 200 SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 259

Query: 63  AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 122
           AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA+RAVAAGVF
Sbjct: 260 AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAFRAVAAGVF 319

Query: 123 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 182
           VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRV+LGTSVYGGPAL+PG
Sbjct: 320 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVILGTSVYGGPALVPG 379

Query: 183 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 242
           R++PLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIV+CDRGINSRAAKGEVVKKAGGLGM
Sbjct: 380 RMHPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVVCDRGINSRAAKGEVVKKAGGLGM 439

Query: 243 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 302
           ILANGVFDGEGLVADCHVLPATAVGA+GGDEIRKYIAEAAKS   PTATILFKGTRLGVR
Sbjct: 440 ILANGVFDGEGLVADCHVLPATAVGAAGGDEIRKYIAEAAKSKSPPTATILFKGTRLGVR 499

Query: 303 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 362
           PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT
Sbjct: 500 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 559

Query: 363 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 422
           SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA
Sbjct: 560 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 619

Query: 423 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 482
           GHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQVITGKIADCSGAKRAGHTGNLNY
Sbjct: 620 GHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQVITGKIADCSGAKRAGHTGNLNY 679

Query: 483 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 542
           PSL+VVFQQYGKHKMSTHFIR+VTNVGDANSIYKVTIKPP GISVTVEPEKLAFRRVGQK
Sbjct: 680 PSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPRGISVTVEPEKLAFRRVGQK 739

Query: 543 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 590
           LSFLVRVQAMA+RLS GSSSMKSGSI+WTDGKH VTSPLVVTMQQPLQ
Sbjct: 740 LSFLVRVQAMALRLSSGSSSMKSGSIVWTDGKHVVTSPLVVTMQQPLQ 787

BLAST of Cucsat.G3662 vs. ExPASy TrEMBL
Match: A0A0A0KAP0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G448090 PE=3 SV=1)

HSP 1 Score: 1155 bits (2987), Expect = 0.0
Identity = 587/588 (99.83%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 3   SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 62
           SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL
Sbjct: 190 SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 249

Query: 63  AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 122
           AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF
Sbjct: 250 AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 309

Query: 123 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 182
           VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG
Sbjct: 310 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 369

Query: 183 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 242
           RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM
Sbjct: 370 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 429

Query: 243 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 302
           ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR
Sbjct: 430 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 489

Query: 303 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 362
           PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT
Sbjct: 490 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 549

Query: 363 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 422
           SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA
Sbjct: 550 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 609

Query: 423 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 482
           GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGH+GNLNY
Sbjct: 610 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHSGNLNY 669

Query: 483 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 542
           PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK
Sbjct: 670 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 729

Query: 543 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 590
           LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ
Sbjct: 730 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 777

BLAST of Cucsat.G3662 vs. ExPASy TrEMBL
Match: A0A5D3BYA5 (Subtilisin-like protease SBT1.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001610 PE=3 SV=1)

HSP 1 Score: 1149 bits (2973), Expect = 0.0
Identity = 584/588 (99.32%), Postives = 586/588 (99.66%), Query Frame = 0

Query: 3   SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 62
           SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL
Sbjct: 190 SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 249

Query: 63  AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 122
           AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF
Sbjct: 250 AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 309

Query: 123 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 182
           VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG
Sbjct: 310 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 369

Query: 183 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 242
           RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM
Sbjct: 370 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 429

Query: 243 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 302
           ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSH  PTATILFKGTRLGVR
Sbjct: 430 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHSPPTATILFKGTRLGVR 489

Query: 303 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 362
           PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT
Sbjct: 490 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 549

Query: 363 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 422
           SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA
Sbjct: 550 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 609

Query: 423 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 482
           GHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQVITGKIADCSGAKRAGHTGNLNY
Sbjct: 610 GHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQVITGKIADCSGAKRAGHTGNLNY 669

Query: 483 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 542
           PSL+VVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK
Sbjct: 670 PSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 729

Query: 543 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 590
           LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ
Sbjct: 730 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 777

BLAST of Cucsat.G3662 vs. ExPASy TrEMBL
Match: A0A1S3CI11 (subtilisin-like protease SBT1.5 OS=Cucumis melo OX=3656 GN=LOC103500650 PE=3 SV=1)

HSP 1 Score: 1148 bits (2969), Expect = 0.0
Identity = 583/588 (99.15%), Postives = 585/588 (99.49%), Query Frame = 0

Query: 3   SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 62
           SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL
Sbjct: 190 SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 249

Query: 63  AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 122
           AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF
Sbjct: 250 AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 309

Query: 123 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 182
           VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG
Sbjct: 310 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 369

Query: 183 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 242
           RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM
Sbjct: 370 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 429

Query: 243 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 302
           ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSH  PTATILFKGTRLGVR
Sbjct: 430 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHSPPTATILFKGTRLGVR 489

Query: 303 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 362
           PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT
Sbjct: 490 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 549

Query: 363 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 422
           SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA
Sbjct: 550 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 609

Query: 423 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 482
           GHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQVITGKIADCSGAKRAGHTGNLNY
Sbjct: 610 GHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQVITGKIADCSGAKRAGHTGNLNY 669

Query: 483 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 542
           PSL+VVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK
Sbjct: 670 PSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 729

Query: 543 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 590
           LSFLVRVQAMAVRLSPGSSSMK GSIIWTDGKHEVTSPLVVTMQQPLQ
Sbjct: 730 LSFLVRVQAMAVRLSPGSSSMKGGSIIWTDGKHEVTSPLVVTMQQPLQ 777

BLAST of Cucsat.G3662 vs. ExPASy TrEMBL
Match: A0A6J1JY93 (subtilisin-like protease SBT1.5 OS=Cucurbita maxima OX=3661 GN=LOC111488559 PE=3 SV=1)

HSP 1 Score: 1128 bits (2917), Expect = 0.0
Identity = 570/588 (96.94%), Postives = 582/588 (98.98%), Query Frame = 0

Query: 3   SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 62
           SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL
Sbjct: 200 SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 259

Query: 63  AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 122
           AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA+RAVAAGVF
Sbjct: 260 AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAFRAVAAGVF 319

Query: 123 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 182
           VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRV+LGTSVYGGPAL+PG
Sbjct: 320 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVILGTSVYGGPALVPG 379

Query: 183 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 242
           R++PLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIV+CDRGINSRAAKGEVVKKAGGLGM
Sbjct: 380 RMHPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVVCDRGINSRAAKGEVVKKAGGLGM 439

Query: 243 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 302
           ILANGVFDGEGLVADCHVLPATAVGA+GGDEIRKYIAEAAKS   PTATILFKGTRLGVR
Sbjct: 440 ILANGVFDGEGLVADCHVLPATAVGAAGGDEIRKYIAEAAKSKSPPTATILFKGTRLGVR 499

Query: 303 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 362
           PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT
Sbjct: 500 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 559

Query: 363 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 422
           SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA
Sbjct: 560 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 619

Query: 423 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 482
           GHVHPQKAMDPGLIYDLNTYDYVDFLCN+NYTTKNIQVITGKIADCSGAKRAGHTGNLNY
Sbjct: 620 GHVHPQKAMDPGLIYDLNTYDYVDFLCNANYTTKNIQVITGKIADCSGAKRAGHTGNLNY 679

Query: 483 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 542
           PSL+VVFQQYGKHKMSTHFIR+VTNVGDANSIYKVTIKPP GISVTVEPEKLAFRRVGQK
Sbjct: 680 PSLSVVFQQYGKHKMSTHFIRSVTNVGDANSIYKVTIKPPRGISVTVEPEKLAFRRVGQK 739

Query: 543 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 590
           LSFLVRVQAMA+RLS GSSSMKSGSI+WTDGKH VTSPLVVTMQQPLQ
Sbjct: 740 LSFLVRVQAMALRLSSGSSSMKSGSIVWTDGKHVVTSPLVVTMQQPLQ 787

BLAST of Cucsat.G3662 vs. ExPASy TrEMBL
Match: A0A6J1HJN0 (subtilisin-like protease SBT1.5 OS=Cucurbita moschata OX=3662 GN=LOC111464184 PE=3 SV=1)

HSP 1 Score: 1116 bits (2886), Expect = 0.0
Identity = 564/588 (95.92%), Postives = 575/588 (97.79%), Query Frame = 0

Query: 3   SGYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 62
           SGYEATNGKMNET+EYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL
Sbjct: 200 SGYEATNGKMNETSEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARL 259

Query: 63  AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVF 122
           AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGA+RAVAAGVF
Sbjct: 260 AAYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAFRAVAAGVF 319

Query: 123 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 182
           VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGR++LGTSVYGGPAL+PG
Sbjct: 320 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRIILGTSVYGGPALVPG 379

Query: 183 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 242
           RLYPLIYAGTEGGDGYSSSLCLEGSLNPN V GKIVLCDRGINSRAAKGEVVKKAGGLGM
Sbjct: 380 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNSVNGKIVLCDRGINSRAAKGEVVKKAGGLGM 439

Query: 243 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 302
           ILANGVFDGEGLVADCHVLPATAVGA+GGDEIRKYIAEA KS   PTATILFKGTRLGVR
Sbjct: 440 ILANGVFDGEGLVADCHVLPATAVGATGGDEIRKYIAEAVKSQSPPTATILFKGTRLGVR 499

Query: 303 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 362
           PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKR  EFNILSGT
Sbjct: 500 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRAIEFNILSGT 559

Query: 363 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 422
           SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA
Sbjct: 560 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 619

Query: 423 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 482
           GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYT KNIQVITGKIADCSGAKRAGHTGNLNY
Sbjct: 620 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTAKNIQVITGKIADCSGAKRAGHTGNLNY 679

Query: 483 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 542
           PSL+VVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPE+LAFRRVGQK
Sbjct: 680 PSLSVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPERLAFRRVGQK 739

Query: 543 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPLQ 590
           LSFLVRVQAMAV+LS GSSSMKSG ++WTDGKH VTS LVVTMQQPLQ
Sbjct: 740 LSFLVRVQAMAVKLSAGSSSMKSGYVVWTDGKHVVTSTLVVTMQQPLQ 787

BLAST of Cucsat.G3662 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 970.3 bits (2507), Expect = 6.9e-283
Identity = 470/589 (79.80%), Postives = 541/589 (91.85%), Query Frame = 0

Query: 4   GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLA 63
           GYEATNGKMNETTE+RSPRDSDGHGTHTASI+AGRYVFPASTLGYA G AAGMAPKARLA
Sbjct: 187 GYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLA 246

Query: 64  AYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVFV 123
           AYKVCWN+GCYDSDILAAFD AV+DGVDV+SLSVGGVVVPYYLDAIAIGA+ A+  G+FV
Sbjct: 247 AYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFV 306

Query: 124 SASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPGR 183
           SASAGNGGPG LTVTNVAPW+TTVGAGT+DRDFPA+VKLGNG+++ G SVYGGP L PGR
Sbjct: 307 SASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGR 366

Query: 184 LYPLIYAGT-EGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 243
           +YPL+Y G+  GGDGYSSSLCLEGSL+PNLVKGKIVLCDRGINSRA KGE+V+K GGLGM
Sbjct: 367 MYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGM 426

Query: 244 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSH--LQPTATILFKGTRLG 303
           I+ANGVFDGEGLVADCHVLPAT+VGASGGDEIR+YI+E++KS     PTATI+FKGTRLG
Sbjct: 427 IIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLG 486

Query: 304 VRPAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILS 363
           +RPAPVVASFSARGPNPE+PEI+KPDVIAPGLNILAAWPD+IGPSG+ +D R TEFNILS
Sbjct: 487 IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILS 546

Query: 364 GTSMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDF 423
           GTSMACPHVSGLAALLKAAHP WSPAAI+SAL+TTAYT+DN GE M+DES+GNTS+V+D+
Sbjct: 547 GTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDY 606

Query: 424 GAGHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNL 483
           G+GHVHP KAMDPGL+YD+ +YDY++FLCNSNYT  NI  IT + ADC GA+RAGH GNL
Sbjct: 607 GSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNL 666

Query: 484 NYPSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVG 543
           NYPS +VVFQQYG+ KMSTHFIRTVTNVGD++S+Y++ I+PP G +VTVEPEKL+FRRVG
Sbjct: 667 NYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVG 726

Query: 544 QKLSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPL 590
           QKLSF+VRV+   V+LSPG++++++G I+W+DGK  VTSPLVVT+QQPL
Sbjct: 727 QKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

BLAST of Cucsat.G3662 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 636.3 bits (1640), Expect = 2.4e-182
Identity = 329/588 (55.95%), Postives = 426/588 (72.45%), Query Frame = 0

Query: 7   ATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLAAYK 66
           A  G +N+T E+ SPRD+DGHGTHT+S AAGR+ F AS  GYA G A G+APKAR+AAYK
Sbjct: 185 AVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYK 244

Query: 67  VCW-NAGCYDSDILAAFDAAVSDGVDVVSLSVG---GVVVPYYLDAIAIGAYRAVAAGVF 126
           VCW ++GC DSDILAAFDAAV DGVDV+S+S+G   G+  PYYLD IAIG+Y A + G+F
Sbjct: 245 VCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIF 304

Query: 127 VSASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPG 186
           VS+SAGN GP G++VTN+APWVTTVGA T+DR+FPAD  LG+G  + G S+Y G  L  G
Sbjct: 305 VSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPL-NG 364

Query: 187 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 246
           R++P++Y G  G    S+SLC+E +L+P  V+GKIV+CDRG + R AKG VVKKAGG+GM
Sbjct: 365 RMFPVVYPGKSGMS--SASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGM 424

Query: 247 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 306
           ILANG  +GEGLV D H++PA AVG++ GD I+ Y    A SH  P A+I F+GT +G++
Sbjct: 425 ILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAY----ASSHPNPIASIDFRGTIVGIK 484

Query: 307 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 366
           PAPV+ASFS RGPN  SPEI+KPD+IAPG+NILAAW D +GP+G+P+D R TEFNILSGT
Sbjct: 485 PAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGT 544

Query: 367 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 426
           SMACPHVSG AALLK+AHP WSPA I+SA+MTT   +DN   +++DES+G ++T  D+G+
Sbjct: 545 SMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGS 604

Query: 427 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 486
           GH++  +AM+PGL+YD+   DY+ FLC+  Y  K IQVIT     C   ++    GNLNY
Sbjct: 605 GHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPS-PGNLNY 664

Query: 487 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 546
           PS+  VF    +  +S   IRT TNVG A ++Y+  I+ P G++VTV+P +L F    ++
Sbjct: 665 PSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKR 724

Query: 547 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTD-GKHEVTSPLVVTMQQPL 590
            S+ V V      +  G +    GS+ W D GKH V SP+VVT    L
Sbjct: 725 RSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

BLAST of Cucsat.G3662 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 579.7 bits (1493), Expect = 2.6e-165
Identity = 311/581 (53.53%), Postives = 398/581 (68.50%), Query Frame = 0

Query: 4   GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLA 63
           GYE+T G ++E+ E RSPRD DGHGTHT+S AAG  V  AS LGYA G A GMAP+AR+A
Sbjct: 189 GYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVA 248

Query: 64  AYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVFV 123
            YKVCW  GC+ SDILAA D A++D V+V+S+S+GG +  YY D +AIGA+ A+  G+ V
Sbjct: 249 VYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILV 308

Query: 124 SASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPGR 183
           S SAGN GP   +++NVAPW+TTVGAGT+DRDFPA   LGNG+   G S++ G AL P +
Sbjct: 309 SCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL-PDK 368

Query: 184 LYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGMI 243
           L P IYAG    +  + +LC+ G+L P  VKGKIV+CDRGIN+R  KG+VVK AGG+GMI
Sbjct: 369 LLPFIYAG-NASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMI 428

Query: 244 LANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVRP 303
           LAN   +GE LVAD H+LPAT VG   GD IR Y+     +   PTA+I   GT +GV+P
Sbjct: 429 LANTAANGEELVADAHLLPATTVGEKAGDIIRHYVT----TDPNPTASISILGTVVGVKP 488

Query: 304 APVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGTS 363
           +PVVA+FS+RGPN  +P I+KPD+IAPG+NILAAW    GP+G+ +D R  EFNI+SGTS
Sbjct: 489 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTS 548

Query: 364 MACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGAG 423
           M+CPHVSGLAALLK+ HP WSPAAI+SALMTTAY     G+ +LD ++G  ST  D GAG
Sbjct: 549 MSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAG 608

Query: 424 HVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNYP 483
           HV P  A +PGLIYDL T DY+ FLC  NYT+  I+ ++ +   C  +K +    +LNYP
Sbjct: 609 HVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSK-SYSVADLNYP 668

Query: 484 SLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQKL 543
           S AV     G +K    + RTVT+VG A +         +G+ ++VEP  L F+   +K 
Sbjct: 669 SFAVNVDGVGAYK----YTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKK 728

Query: 544 SFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVT 585
           S+ V      V  S  S S   GSI W+DGKH V SP+ ++
Sbjct: 729 SYTV---TFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Cucsat.G3662 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 576.2 bits (1484), Expect = 2.9e-164
Identity = 309/576 (53.65%), Postives = 399/576 (69.27%), Query Frame = 0

Query: 7   ATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLAAYK 66
           A+ G  +   E  SPRD DGHGTHT++ AAG  V  AS LGYA G A GMA +AR+A YK
Sbjct: 188 ASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYK 247

Query: 67  VCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVFVSAS 126
           VCW+ GC+ SDILAA D A+ DGVDV+SLS+GG   PYY D IAIGA+ A+  GVFVS S
Sbjct: 248 VCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCS 307

Query: 127 AGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIPGRLYP 186
           AGN GP   +V NVAPWV TVGAGT+DRDFPA   LGNG+ + G S+Y G  +    L  
Sbjct: 308 AGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPL-E 367

Query: 187 LIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGMILAN 246
           L+Y     G+  SS+LCL GSL+ ++V+GKIV+CDRG+N+R  KG VV+ AGGLGMI+AN
Sbjct: 368 LVY---NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMAN 427

Query: 247 GVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVRPAPV 306
               GE LVAD H+LPA AVG   GD +R+Y+    KS  +PTA ++FKGT L V+P+PV
Sbjct: 428 TAASGEELVADSHLLPAIAVGKKTGDLLREYV----KSDSKPTALLVFKGTVLDVKPSPV 487

Query: 307 VASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGTSMAC 366
           VA+FS+RGPN  +PEI+KPDVI PG+NILA W D IGP+G+  D R T+FNI+SGTSM+C
Sbjct: 488 VAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSC 547

Query: 367 PHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGAGHVH 426
           PH+SGLA LLKAAHP WSP+AIKSALMTTAY LDN    + D +  + S     G+GHV 
Sbjct: 548 PHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVD 607

Query: 427 PQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNYPSLA 486
           PQKA+ PGL+YD++T +Y+ FLC+ +YT  +I  I  K    + +K+    G LNYPS +
Sbjct: 608 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIV-KRPSVNCSKKFSDPGQLNYPSFS 667

Query: 487 VVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQKLSFL 546
           V+F      K    + R VTNVG A+S+YKVT+     + ++V+P KL+F+ VG+K  + 
Sbjct: 668 VLF----GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYT 727

Query: 547 VR-VQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPL 582
           V  V    V +   ++  + GSI W++ +HEV SP+
Sbjct: 728 VTFVSKKGVSM---TNKAEFGSITWSNPQHEVRSPV 747

BLAST of Cucsat.G3662 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 556.6 bits (1433), Expect = 2.4e-158
Identity = 293/587 (49.91%), Postives = 391/587 (66.61%), Query Frame = 0

Query: 4   GYEATNGKMNETTEYRSPRDSDGHGTHTASIAAGRYVFPASTLGYARGKAAGMAPKARLA 63
           GYEA  GK++E  EY+SPRD DGHGTHTA+  AG  V  A+  G+A G A GMA KAR+A
Sbjct: 204 GYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVA 263

Query: 64  AYKVCWNAGCYDSDILAAFDAAVSDGVDVVSLSVGGVVVPYYLDAIAIGAYRAVAAGVFV 123
           AYKVCW  GC+ SDIL+A D AV+DGV V+S+S+GG V  Y  D+++I  + A+  GVFV
Sbjct: 264 AYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFV 323

Query: 124 SASAGNGGPGGLTVTNVAPWVTTVGAGTMDRDFPADVKLGNGRVVLGTSVYGGPALIP-G 183
           S SAGNGGP  +++TNV+PW+TTVGA TMDRDFPA VK+G  R   G S+Y G  ++P  
Sbjct: 324 SCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKN 383

Query: 184 RLYPLIYAGTEGGDGYSSSLCLEGSLNPNLVKGKIVLCDRGINSRAAKGEVVKKAGGLGM 243
           + YPL+Y G        +S CL+G+L+   V GKIV+CDRG+  R  KG+VVK+AGG+GM
Sbjct: 384 KQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGM 443

Query: 244 ILANGVFDGEGLVADCHVLPATAVGASGGDEIRKYIAEAAKSHLQPTATILFKGTRLGVR 303
           +L N   +GE LVAD H+LPA AVG   G  I++Y   + K+    TA++   GTR+G++
Sbjct: 444 VLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKA----TASLEILGTRIGIK 503

Query: 304 PAPVVASFSARGPNPESPEIVKPDVIAPGLNILAAWPDKIGPSGIPTDKRTTEFNILSGT 363
           P+PVVA+FS+RGPN  S EI+KPD++APG+NILAAW   + PS + +D R  +FNILSGT
Sbjct: 504 PSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGT 563

Query: 364 SMACPHVSGLAALLKAAHPGWSPAAIKSALMTTAYTLDNRGETMLDESSGNTSTVLDFGA 423
           SM+CPHVSG+AAL+K+ HP WSPAAIKSALMTTAY  DN  + + D S    S+  D GA
Sbjct: 564 SMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGA 623

Query: 424 GHVHPQKAMDPGLIYDLNTYDYVDFLCNSNYTTKNIQVITGKIADCSGAKRAGHTGNLNY 483
           GH+ P +A DPGL+YD+   +Y +FLC  + +   ++V T           A + GNLNY
Sbjct: 624 GHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNY 683

Query: 484 PSLAVVFQQYGKHKMSTHFIRTVTNVGDANSIYKVTIKPPSGISVTVEPEKLAFRRVGQK 543
           P+++ +F +   H  +    RTVTNVG   S YKV++ P  G SVTV+P+ L F    QK
Sbjct: 684 PAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQK 743

Query: 544 LSFLVRVQAMAVRLSPGSSSMKSGSIIWTDGKHEVTSPLVVTMQQPL 590
           LS+ V  +       P     + G ++W    H+V SP+++T   PL
Sbjct: 744 LSYTVTFRTRFRMKRP-----EFGGLVWKSTTHKVRSPVIITWLPPL 780