Cucsat.G21255 (gene) Cucumber (B10) v3

Overview
NameCucsat.G21255
Typegene
OrganismCucumis sativus L. var. sativus cv B10 (Cucumber (B10) v3)
DescriptionNPL4-like protein 1
Locationctg910: 63514 .. 69815 (+)
RNA-Seq ExpressionCucsat.G21255
SyntenyCucsat.G21255
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACGTGAAGATTAAAACTGTGAAAACACACTTTATTCCCAACTAAAAAGGTAAATTTCCCAAATATGATTAGAAAAAAAACTAGAAATCGTCTAGCAAAGTGGAAGGGTCGAGGGGGTTATTTGGTCATCGAATTCCGCCGTGTTAACGGAGATTGTCTATTTCCTGATTCCCAAGCGACCGCGGTATACTTCCGATTTGATATAAATACAATCCCTTGCCTCTACTCCCTTAACAAGAAATTCCCAGAAGATCGTCATACATACCCTTCAAACTTCTCTCTAAATTTCCGTCAGCATTTTCTGAATCCCAACGTCGACTGGAAGTATAGTTGTTTGTTCATCAAATCTCTCTCTAAATAACGATCAATCTCCTCTTCCTTATACAAATTCACGTTACAACTCCTGCCGACACCAATATGATGCTCAGAATTCGCAGTAGAGATGGTCTGGAGCGAGTCGCTGTAGAAAACCCACACATCACAATCGCTCAACTCAAAGCCATAATCCAATCCCAGCTCAAAATCCCAATCCACAACCAAACCCTCTCCACTAACCAAAATATTCTATTGGCTAAGACTCAGGATGATCTTTCCAAATTCACTGACATGTCTAATCCTAATACCTACCTCTCCTCGCTTAATTTGTCCCATGGGTCTATTGTCTTTCTCGCCTACGAGGGCGAGCGCACTGTTGCTGGGCCTACTGTCCATCCCGCCGGATCTTTTGGCCGTAAAATGACTATGGATGATCTAATTGCTAAGCAGATGCGGATCACTCGTCAAGAAAACCCGCACTGTGAATTGGTTTCTTTCGACCGGGATTGCGCCAATGCTTTCCAGCATTATGTTAATGAAACGCTAGCCTTCGCCGTCAAACGTGGGGGGATGATGTACGGAACCGTATCACCAGAAGGCAAGGTCGAGGTAGATTTCATATATGAGCCGCCACAGCAAGGGACTGAGGATAATTTACTGTTTTTCCGAGATCACGATGAGGAAAGATTGGTGGAAGCAATCGCAGTTGGGTTGGGAATGAGGAAAGTTGGGTTTATATTCACGCAGACGATAAGTCAGGACAAAAAAGACTACACCTTATCCAACAGGGAAGTACTCCAGGCGGCTCAGTTTCACTCTGAGAGCGAGTTGAAGGAGTGGGTGACAGCAGTTGTGAAGTTGGAGGTGAACGAGGATGGGGGTGCTGATGTTCATTTTGAGGCTTTTCAAATGAGTGACATGTGTATTAGATTGTTTAAGGAATGTTGGTTTGAGACGGATATTGGAGAGGATTTTGATCCCAAGCTCTCCAAGATGAAGAAGGACGTTGTTGTTGGTGTCAAAGACACTAGAGATGTTGACAATGATTTTTTCCTGGTCGTCGTCAAGATCCTCGACCATCAGGTTCGTGGACCCTTTATTTTTAATAATCGTATTCCCTCATTCCTCTGCTGTGTCGAACTGTGTGTGTTTTCTCTTCAAATAATGACTCATAATTGCATTCATTGAAATTGTTTCCACTGAATTCTCAGATTTTTGTAAATATTCTTTGTATGTTGCTTCTTTTACACATTTGACTTACCGGGATATCATGATTGAAAGTGAATTGTCAACTTGCTTTGTGAACTGAATGTGTTAGCTATTGGCCGGAGCGTAGGAGTTGGAGGTTTAGGGGGTGGTGTATTTTCCATCTTCTTAGGGCCAGAATATTAGGTAATAATTTTTGGAAATATCAATTTGGTAATTCGTTCATTGCATATTTGCTATTTATTCTACCATAACAGGTTTAACAGATAACTCGGATCAAGTAAGATATAACTGTTGCTTCTATTTTCTCTCTAAGTTCTTCGTTCTTGGGAACCATGGGCCAAGTTGGACCTTCAATGTTGTTTCCAGCTCAAGTTACCAAGGCCTAGACTCCATTCTACTCTTTCGTCCACATCTAGATTGGGGAAGAATGAGAATTGCCTTTTTAAAATTCACTAGTTGCTGCCTACCTCTCCTTTTTCTTTTCAATTCTAGCCCCCTCTTGAAACTGAATTCCTGAGCTCCATAATGATGGTATTTACAATGGGAAAAAGCTTCGTAATTCCTTCTAGTATAACATTTTATCTCTTGGGATCTATTATGTTTTTCTGGCTTCTTTTTCATACAAGAATTAACTGTACTTTTATGCTTTATGTTTCTTCACCTTTTTTATCCTCTTAGGAGCTGCTTGACTGTGTATGTTCAAAAAGTTCATACAGCATCCTTGTTTACTAAGTAAATTTTGTTGCATTAATCGTTCCCAAATGATGTTCTTATTACCGATGGGGCTAAGGTGTTGTTTACAAGTTATTATATCAGTCTTGTCTCTCAATGTCCATATCGTTAAATAATGCATTGGCTAACCAATCATTTCATGTCGAGCTCCTTAATCTCTATAGGGAAAATAAAACGTCACATGATGGTGAAAATATGGAAACTTTGGCCATCCCTTCAGTAGAGGGGAAAGCTATGTTAACCTCATGGATGAGGGTCTTGCTAAATACATCAAAGAGGCTGTTATATCTCTTCTCCCATTCATTCCATCTCAATAATGATGATTATAGGGCTTATTTCTTCAACTCTTAATAGGCCTTTTTTGTTATACGCTTGTTATGAAAATCATTATTGGTTTCCTATTAAGCCCAAAGATATTTTGTGGACTGAAGTTCCAAAAGGACTTGAAGCTCTAAGAAGACGAAGTTTTGTTTTCTCAAGATTTTCTTTTTCCTGTTTCTGGCATGGTGTTCTGTGCTTCTTGGCATTCAATGTTCCATCCGTTCCCCCCTTCTATGACGTACGAAATAATTTATTCCAGTTTCTTGTCAGTTCAGTTCTCTGTTCTCAAACTTACCTTCCCTGGGGCTTCATATCCATAGACATATACCAGTGTTGCTGGTAACAGCTTCCATTAGCTTTTCTTTTCTTTCCTTCCATATTTGCGGCTACTAAAAAGTTTCCTCTTAATGACCGATGATCCCACTTTGAGGATCCAACCTTTGATATGCCAGATCTTTAGCTCATTTACCTGCTGTCTTTGAATGCAGGCGGATTACAAGTCATTCTCCCCTCACTCACAAACCAAATTTTCTGTGCTGATATTTTTTGCTGGCCTTTATACTTTCCAATTTCTTTTCTACCCACACTGTGAACAAACTCAACCTTGGGCCACTTAGTGATCTTCCTTCTTTTCTTGCTGTCGGTGCACCATCCTGATTATCAACCTTCCTGGTATCGTCTTTCTTGCCATATGACACACTTGATTTTGTTATGGAGCTCTACCTCTTTGTCTTTGCCAACCTAATCTCTCTCCCTCTCTCTTTTTAATTGTTTATTCTTCACTTTTATTTCTCCAAAATTTTTCACTATTGACACAGATTGAAATATCAAATACATTTACTCCAATTGATTCTTCAGTTGATCCATTTCTTTCGATAAATCTTCAATATCACTTAACGTAAATTAACCTCTAACTCTATATCTGACTCCATGACCAGAAATGAATTACACTATATCAATTGATTGAATCCAAATATGCTAAAAAAAAATATAAAAGGTGACAAAATTGTAAGATAACCAAGTAATTCAAGGTACTTGGACCTCCTTCATTTAATCACACTCACTTAAAGCTTAGCCGACTTAATTTGTCCATGCTCTCATCTTTTCTAATCCCCTCTATTTATAACACACTCTACTATCTTTCTCACAATTATCAATATACTCTCTTGACCTCTCGTTCTCTTGGTGGATTGGGAGCGCAACCTTGAAGGAAAAGGAAAAACTCTGTTTTTCTCAAATGGTAGCACTTCTAGAAAGAAAATTAGCAGCTCTTTGGGAAAGGGCGGTTAAGAGGAAATATGGCGTTGATTCTTCAAAAGACATGAAAAACAAGACAAATTTGAGAGGTTTTTATTTGTTTAGTCCTTAGAAGGTGATAGCTAAAAACTCAGATTTCTTTTGGAAAAACATTAGTTTTAAACTAGGTGATAATGACCATGTTTTAGGAAGACAGGCTTGTTAATCAGGCACCTTACAAAAGTTGGTTCCAATTTGCAACAATATAATTTAGGCTAAGATCTGTATGGGGCTGTTGGTGTTTACACCAAAAGGCATGTTAAAAATAGATTAATCAAGGATGTCTATTTGTCCATGGAAGAAGTGATTGATTCTTAGATTTAGGGAGGTGCCCGTTTTTGTTCATTTTTTCTGTATGAAAGTTCTTGTAAGAAAAGTACTTAAAAAGGTTAGATTCTTCCTTTGGACGGTTACTCCCGGCATTAATACTTACGGTAAGGTGCGAAGATAACTTCACCTTAACATTTTTCTTGTAATCCTATTACTTGTGCCAACTGCAGGAGTGAGGGATCACTTCCTCACTTTTTACTTCACTGCAGCTTTGGCAAAAAAGTCTGCAACTACTTTGGTAACTTATTTGGTCTAATATGGTGTTACCCTCTAGTGTTAGATCAAGGGCTGGTGGAGTTTTACTGTTGTGGTTAACAAAGTAATGTAATATGTTTTGGTTCCTTTACTTAATGTACTCTGTTTCTACAGTTTATTTTTCGTTCCAACTTTTGTTCTTTTATTTAGGAGGCTTATTTGTTTGGAGCGTTTGCAAAAATAGCAAAAAAAATTATGATAATATGGTCCATATCACTACATTTTCTAAATTACTTGTTATATTTGTCATATTTGCAATATGCAAAAATGAGGTACTATGAGCTGTTTTTTCTAAAAAAAAATTGTCATTTGATGCAATTTTCCTATTGTTTTCATTGGAGGCTCACCAGCCACCTTCTTTGTAGAACCAGAAGCCCTTCTTCCTCTTGGAAGGATCATTAAGTAAATTTATCTTCTGTGTTTAGAACACAAGGGCTGTTTTGTGGCTAATGTAGAAGGAAAGAAATGTTAGAATTCGTAGACACGTACAATTTCTTTAATTATTTTTGACTTTGTACAGTTCACGGCTTCCAATTGGAGTAGCTGAACACAATTTCCTTTTTTTTTGTAATTGAAGGCTCTTATGAATTTTAATGCTTCTCTTTGGATGTTGTTGACGGTCCCTTTTTTCATTATATGCTAGGTCTAGGACATATTGGACATATTTTATTAGATTGGAGTCCTTTTCTCTAGGGTATTCCCTTTTTGTGGGCTGGCTCTTTTTTTTCTCATTGTTTTATGCCCTTATATTCTTTCGATTTTTTCATTGAAATTTGGTCTTAAGAACGAAAAAAAAACAGTTGTTTTTTAATTACTCCACCGGTTCACCCTGCTCATTAATTTTCTTTTTCTTAAGAAATAAAAAATAAAAAACTGTTATACCCTAATTCTATTCTGGTAGCACCCCTCGCATGTAGCTGCTAGTGTATTGGTTTGATCTTTCATTTTTCTTTCGGTGTGTGTGCCAAATATCCTGCTATGTGTTTGAGCGTTTAAATGTGATTATGAAGTTGGATGTGAATCGGCCAACAATTGTAACATTTCTAGTAGATGTGGAGGGTAATTTGATGCGAGAATTGGCCCAAATTTAAACATACAATTGTGCTTGATGAATAATATGAAAATAGGAAAAAAGAACTGCAGACAAGTCAAGTATTCTTCATTTTTCTAACTTGATACTTCAAATATAAAAAAGGTAGAAAATTTTAAATTTGGCCGCTTCTAACTGATGTTGATGACAACTTTGTACTGCAGGGTCCACTTTCGACAACATTTCCTATTGAAAACCGAAATGTTCCAGTGACCATGAAGGCATTGAAGAATCACCTAGACCGTTCAAAAGGGCTTCCGTTTGTGAAGCGAATTTCCGATTTTCATTTGCTACTGTTACTAGCCAGGGTGTTAGACGTGAGCTCTGACGTTCCTGCACTGGCTGAGTGCGTTCAGACCCAGACAGGCGTACCTGAAGGTTACAAAATATTAATCGAGTCTATGGCAAGTGCTGCTTGATATACATAGTGTCAACTATGACTGGTTACAGGAGCTTGTTCTTGATTCTCAGTTACTTTCTATCGTCTCTCTTATCAACTTAGAGATTGTAGTTATATTTTAACATACTGTCATCTAAAAATGGTTTTATGATAATTTATGTCATAACAAAATGCCTTCTTATGTCGTAACAAAATGGCTTCCATAAAAAGTCTTGTCTTGGACTAAAACTTCACGAGTAATGCTATCTATGGAGTTTTGGTCTTAAACTTTTAATTACTTACCTTATTGCTAAAATGGGTTTTTCCTAACTAAAATTTTTGTTTGTATAGTTTTTTTTTCTACTGGATAAAAATA

Coding sequence (CDS)

ATGATGCTCAGAATTCGCAGTAGAGATGGTCTGGAGCGAGTCGCTGTAGAAAACCCACACATCACAATCGCTCAACTCAAAGCCATAATCCAATCCCAGCTCAAAATCCCAATCCACAACCAAACCCTCTCCACTAACCAAAATATTCTATTGGCTAAGACTCAGGATGATCTTTCCAAATTCACTGACATGTCTAATCCTAATACCTACCTCTCCTCGCTTAATTTGTCCCATGGGTCTATTGTCTTTCTCGCCTACGAGGGCGAGCGCACTGTTGCTGGGCCTACTGTCCATCCCGCCGGATCTTTTGGCCGTAAAATGACTATGGATGATCTAATTGCTAAGCAGATGCGGATCACTCGTCAAGAAAACCCGCACTGTGAATTGGTTTCTTTCGACCGGGATTGCGCCAATGCTTTCCAGCATTATGTTAATGAAACGCTAGCCTTCGCCGTCAAACGTGGGGGGATGATGTACGGAACCGTATCACCAGAAGGCAAGGTCGAGGTAGATTTCATATATGAGCCGCCACAGCAAGGGACTGAGGATAATTTACTGTTTTTCCGAGATCACGATGAGGAAAGATTGGTGGAAGCAATCGCAGTTGGGTTGGGAATGAGGAAAGTTGGGTTTATATTCACGCAGACGATAAGTCAGGACAAAAAAGACTACACCTTATCCAACAGGGAAGTACTCCAGGCGGCTCAGTTTCACTCTGAGAGCGAGTTGAAGGAGTGGGTGACAGCAGTTGTGAAGTTGGAGGTGAACGAGGATGGGGGTGCTGATGTTCATTTTGAGGCTTTTCAAATGAGTGACATGTGTATTAGATTGTTTAAGGAATGTTGGTTTGAGACGGATATTGGAGAGGATTTTGATCCCAAGCTCTCCAAGATGAAGAAGGACGTTGTTGTTGGTGTCAAAGACACTAGAGATGTTGACAATGATTTTTTCCTGGTCGTCGTCAAGATCCTCGACCATCAGGGTCCACTTTCGACAACATTTCCTATTGAAAACCGAAATGTTCCAGTGACCATGAAGGCATTGAAGAATCACCTAGACCGTTCAAAAGGGCTTCCGTTTGTGAAGCGAATTTCCGATTTTCATTTGCTACTGTTACTAGCCAGGGTGTTAGACGTGAGCTCTGACGTTCCTGCACTGGCTGAGTGCGTTCAGACCCAGACAGGCGTACCTGAAGGTTACAAAATATTAATCGAGTCTATGGCAAGTGCTGCTTGA

Protein sequence

MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSKFTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRITRQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQGTEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSESELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKKDVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPFVKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA
Homology
BLAST of Cucsat.G21255 vs. ExPASy Swiss-Prot
Match: Q9LYC2 (NPL4-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At3g63000 PE=1 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 4.0e-181
Identity = 305/411 (74.21%), Postives = 365/411 (88.81%), Query Frame = 0

Query: 2   MLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSKF 61
           MLR+RSRDGLERV+V+ PHIT++QLK +IQ QL+IPIHNQTLSTN+N+LLAK+  D   F
Sbjct: 3   MLRVRSRDGLERVSVDGPHITVSQLKTLIQDQLQIPIHNQTLSTNRNLLLAKSPSDFLAF 62

Query: 62  TDMSNPNTYLSSLNLSHGSIVFLAYEGERTV-AGPTVHPAGSFGRKMTMDDLIAKQMRIT 121
           TDM++PN  +SSLNL+HGS+V+LAYEGERT+  GP V PAGSFGRKMT++DLIA+QMR+ 
Sbjct: 63  TDMADPNLRISSLNLAHGSMVYLAYEGERTIRGGPAVTPAGSFGRKMTVEDLIARQMRVG 122

Query: 122 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 181
           RQE  HC+ VSFDRDCANAFQH+VNE+LAFAVKRGG MYG VS +G+VEV+FIYEPPQQG
Sbjct: 123 RQEKAHCDSVSFDRDCANAFQHFVNESLAFAVKRGGFMYGNVSEDGQVEVNFIYEPPQQG 182

Query: 182 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 241
            EDNL+  RD +EE+ V+AIA+GLGMR+VGFIF QT++QDKK+YTLSN EVL AAQ H+E
Sbjct: 183 MEDNLILMRDSEEEKRVDAIALGLGMRRVGFIFNQTVTQDKKEYTLSNVEVLLAAQLHAE 242

Query: 242 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 301
           SELKEWVTAVVKLE+NEDGGADVHFE FQMSDMC+RLFKE WFET+IG + DPKLSK+KK
Sbjct: 243 SELKEWVTAVVKLEINEDGGADVHFEPFQMSDMCVRLFKEGWFETEIGPEDDPKLSKLKK 302

Query: 302 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 361
           +VVVGVKD ++VDNDFFLV+VKILDHQGPLS TFPIENRN   TM+ALK H++R++ LPF
Sbjct: 303 EVVVGVKDVKEVDNDFFLVLVKILDHQGPLSCTFPIENRNTQTTMRALKTHMERARSLPF 362

Query: 362 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 412
           VKRISDFHLLL +A+ LDVSSDVPALAECV+ Q+ VPEGY++LI+SMA+ +
Sbjct: 363 VKRISDFHLLLFVAQFLDVSSDVPALAECVRLQSHVPEGYELLIDSMANTS 413

BLAST of Cucsat.G21255 vs. ExPASy Swiss-Prot
Match: O82264 (NPL4-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At2g47970 PE=1 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 5.4e-178
Identity = 308/410 (75.12%), Postives = 359/410 (87.56%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERV  E  HIT++QLK +I  QL+IP+H QTLSTN+++LLAKT  DL  
Sbjct: 2   MMLRIRSRDGLERVTAEGAHITVSQLKTLIADQLQIPLHKQTLSTNRDLLLAKTPADLLA 61

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAG-PTVHPAGSFGRKMTMDDLIAKQMRI 120
           FTD+++PN  LSSLNL HGS+++LAY+GER++ G P V PAGSFGRKMT+DDLIA+QMR+
Sbjct: 62  FTDLTDPNLPLSSLNLGHGSMLYLAYDGERSIPGAPPVTPAGSFGRKMTVDDLIARQMRV 121

Query: 121 TRQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQ 180
           TRQE  HC+ VSFDRD ANAFQHYVNE+LAFAVKRGG MYGTV+ EG+VEVDFIYEPPQQ
Sbjct: 122 TRQETSHCDSVSFDRDAANAFQHYVNESLAFAVKRGGFMYGTVTEEGQVEVDFIYEPPQQ 181

Query: 181 GTEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHS 240
           GTE NL+  RD DEE+ V+AIA+GLGMR+VGFIF QT+ QDK +YTLSN EVLQAA+ H+
Sbjct: 182 GTEANLILMRDADEEKRVDAIAMGLGMRRVGFIFNQTVVQDKTEYTLSNAEVLQAAELHA 241

Query: 241 ESELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMK 300
           ESELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKE WFET+I  D DPKLSKMK
Sbjct: 242 ESELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKEEWFETEIMPDDDPKLSKMK 301

Query: 301 KDVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLP 360
           K+VVVGVKD ++VDNDFFLV+V+ILDHQGPLS+TFPIENR+   TM+ALK HLDR+K LP
Sbjct: 302 KEVVVGVKDLKEVDNDFFLVLVRILDHQGPLSSTFPIENRSSRATMRALKTHLDRAKSLP 361

Query: 361 FVKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMAS 410
            VK++SDFHLLL +A+ LDVSSDVPALAECV+ Q+ VPEGY +LIESMA+
Sbjct: 362 LVKKMSDFHLLLFVAQFLDVSSDVPALAECVRLQSPVPEGYALLIESMAN 411

BLAST of Cucsat.G21255 vs. ExPASy Swiss-Prot
Match: Q9AS33 (NPL4-like protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0377700 PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 2.8e-126
Identity = 239/419 (57.04%), Postives = 306/419 (73.03%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENP-HITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLS 60
           M+LRIRSRDG +R+ V +P   T+  L+ +I +++ +P+  Q LS +  +LL  +    +
Sbjct: 1   MILRIRSRDGTDRITVPDPAAATVGDLQRLIAARVTVPVPLQRLSLDPALLLPSS----A 60

Query: 61  KFTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGP----TVHPAGSFGRKMTMDDLIAK 120
               +++P   LSSL LS+GS V+L+Y  +   + P     +  AGSFG+KMTMDDLIA+
Sbjct: 61  SAALLADPAAPLSSLRLSNGSFVYLSYPPDARSSQPPPPKALSAAGSFGKKMTMDDLIAR 120

Query: 121 QMRITRQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGK-VEVDFIY 180
           Q+R+TRQE P C   SFDRD ANAFQ +V E+LAFA KR G +YG V  + K V VDFIY
Sbjct: 121 QIRVTRQEAPLCAAASFDRDSANAFQLHVAESLAFATKRAGFLYGRVDADTKEVFVDFIY 180

Query: 181 EPPQQGTEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTI---SQDKKDYTLSNREV 240
           EPPQ GTED +   RD  EE  V+AIA GLGMR+VG +FTQ +   + D  +YT+SNREV
Sbjct: 181 EPPQVGTEDVVQLMRDAQEEARVDAIAHGLGMRRVGLVFTQAVGRKTSDTGEYTMSNREV 240

Query: 241 LQAAQFHSESELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDF 300
           LQA +  +E  + EWVTA+VKLEV +DG  DVHFEAFQMS++C++LFK+   ET+IG+  
Sbjct: 241 LQATELQAEGGIPEWVTAIVKLEVGDDGSGDVHFEAFQMSEICVKLFKDGVLETEIGDKD 300

Query: 301 DPKLSKMKKDVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNH 360
           DP+LSKM+K+VV G KDT +VDNDFFLV VKI DHQGPLST FPIENR  PV M ALK+H
Sbjct: 301 DPRLSKMRKEVVAGGKDTMEVDNDFFLVPVKISDHQGPLSTGFPIENRGNPVAMSALKSH 360

Query: 361 LDRSKGLPFVKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASA 411
           LDR+K LPFVKRISDFHLLLL+A  LD+ +DVPAL  CV+ Q+ VPEGY++LIES+A A
Sbjct: 361 LDRAKHLPFVKRISDFHLLLLVAAFLDIKADVPALTACVKNQSVVPEGYQLLIESLAGA 415

BLAST of Cucsat.G21255 vs. ExPASy Swiss-Prot
Match: Q8TAT6 (Nuclear protein localization protein 4 homolog OS=Homo sapiens OX=9606 GN=NPLOC4 PE=1 SV=3)

HSP 1 Score: 95.5 bits (236), Expect = 1.5e-18
Identity = 93/331 (28.10%), Postives = 149/331 (45.02%), Query Frame = 0

Query: 119 ITRQENPHCELVSFD-RDCANAFQHYVNETLAFAVKRGGMMYGTVS-----PEG-KVEVD 178
           + RQ+  H + + F+    A+ F  +  +T     +  G +YG  +     P G + EV 
Sbjct: 215 LNRQKYRHVDNIMFENHTVADRFLDFWRKT---GNQHFGYLYGRYTEHKDIPLGIRAEVA 274

Query: 179 FIYEPPQQGTEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQD----------- 238
            IYEPPQ GT+++L    D   E +V+ IA  LG+RKVG+IFT  +S+D           
Sbjct: 275 AIYEPPQIGTQNSLELLEDPKAE-VVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRN 334

Query: 239 KKDYTLSNREVLQAAQFHSESEL-----------KEWVTAVVKLEVNEDGGAD--VHFEA 298
           K  Y LS+ E + A  F ++               ++VTAV        GG D  VHFE 
Sbjct: 335 KDTYFLSSEECITAGDFQNKHPNMCRLSPDGHFGSKFVTAVA------TGGPDNQVHFEG 394

Query: 299 FQMSDMCIRLFKE-----CWFETDIGEDFDPKLSKMKKDVVVGVKD---------TRDVD 358
           +Q+S+ C+ L ++     C    ++G   +    +   DV     D          R + 
Sbjct: 395 YQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLP 454

Query: 359 NDFFLVVVKILDHQGPLST------TFPIENRNV---PVTMKALKNHLDRSKGLPFVKRI 394
            ++ ++ +     + P+ T       FPIENR+V        +L  +L ++    F+  I
Sbjct: 455 VEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTI 514

BLAST of Cucsat.G21255 vs. ExPASy Swiss-Prot
Match: P60670 (Nuclear protein localization protein 4 homolog OS=Mus musculus OX=10090 GN=Nploc4 PE=1 SV=3)

HSP 1 Score: 95.1 bits (235), Expect = 1.9e-18
Identity = 93/331 (28.10%), Postives = 149/331 (45.02%), Query Frame = 0

Query: 119 ITRQENPHCELVSFD-RDCANAFQHYVNETLAFAVKRGGMMYGTVS-----PEG-KVEVD 178
           + RQ+  H + + F+    A+ F  +  +T     +  G +YG  +     P G + EV 
Sbjct: 215 LNRQKYRHVDNIMFENHTVADRFLDFWRKT---GNQHFGYLYGRYTEHKDIPLGIRAEVA 274

Query: 179 FIYEPPQQGTEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQD----------- 238
            IYEPPQ GT+++L    D   E +V+ IA  LG+RKVG+IFT  +S+D           
Sbjct: 275 AIYEPPQIGTQNSLELLEDPKAE-VVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRN 334

Query: 239 KKDYTLSNREVLQAAQFHSESEL-----------KEWVTAVVKLEVNEDGGAD--VHFEA 298
           K  Y LS+ E + A  F ++               ++VTAV        GG D  VHFE 
Sbjct: 335 KDTYFLSSEECITAGDFQNKHPNICRLSPDGHFGSKFVTAVA------TGGPDNQVHFEG 394

Query: 299 FQMSDMCIRLFKE-----CWFETDIGEDFDPKLSKMKKDVVVGVKD---------TRDVD 358
           +Q+S+ C+ L ++     C    ++G   +    +   DV     D          R + 
Sbjct: 395 YQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDIDKFGNEITQLARPLP 454

Query: 359 NDFFLVVVKILDHQGPLST------TFPIENRNV---PVTMKALKNHLDRSKGLPFVKRI 394
            ++ ++ +     + P+ T       FPIENR+V        +L  +L ++    F+  I
Sbjct: 455 VEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTI 514

BLAST of Cucsat.G21255 vs. NCBI nr
Match: XP_004145952.1 (NPL4-like protein 1 [Cucumis sativus] >KGN49825.1 hypothetical protein Csa_000197 [Cucumis sativus])

HSP 1 Score: 816 bits (2109), Expect = 2.68e-298
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK
Sbjct: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120
           FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT
Sbjct: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120

Query: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180
           RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG
Sbjct: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180

Query: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240
           TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE
Sbjct: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240

Query: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300
           SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK
Sbjct: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300

Query: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360
           DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF
Sbjct: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360

Query: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411
           VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA
Sbjct: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411

BLAST of Cucsat.G21255 vs. NCBI nr
Match: XP_008437660.1 (PREDICTED: NPL4-like protein 1 [Cucumis melo])

HSP 1 Score: 805 bits (2079), Expect = 1.00e-293
Identity = 406/411 (98.78%), Postives = 407/411 (99.03%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLS NQNILLAKTQDDLSK
Sbjct: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSANQNILLAKTQDDLSK 60

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120
           FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT
Sbjct: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120

Query: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180
           RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG
Sbjct: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180

Query: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240
           TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE
Sbjct: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240

Query: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300
           SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKE WFETDIGEDFDPKLSKMKK
Sbjct: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKEGWFETDIGEDFDPKLSKMKK 300

Query: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360
           DVVVGVKDTRDVDNDFFLVVVKI DHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF
Sbjct: 301 DVVVGVKDTRDVDNDFFLVVVKIFDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360

Query: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411
           VKRISDFHLLLLL+RVLDVSSDVPALAECVQTQT VPEGYKILIESMASAA
Sbjct: 361 VKRISDFHLLLLLSRVLDVSSDVPALAECVQTQTAVPEGYKILIESMASAA 411

BLAST of Cucsat.G21255 vs. NCBI nr
Match: XP_038874364.1 (NPL4-like protein 1 [Benincasa hispida])

HSP 1 Score: 791 bits (2044), Expect = 2.17e-288
Identity = 399/411 (97.08%), Postives = 402/411 (97.81%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERVAVENPHITIAQLKA+IQSQLKIPI NQTLSTNQNILLAKT DDLSK
Sbjct: 1   MMLRIRSRDGLERVAVENPHITIAQLKAVIQSQLKIPIQNQTLSTNQNILLAKTHDDLSK 60

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120
           FTDMSNPNT+LSSLNLSHGSIVFLAYEGERTVAGPT HPAGSFGRKMTMDDLIAKQMRIT
Sbjct: 61  FTDMSNPNTHLSSLNLSHGSIVFLAYEGERTVAGPTFHPAGSFGRKMTMDDLIAKQMRIT 120

Query: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180
           RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG
Sbjct: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180

Query: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240
           TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSN+EVLQAAQFHSE
Sbjct: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNKEVLQAAQFHSE 240

Query: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300
           SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKE WFETDIGEDFDPKLSKMKK
Sbjct: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKEGWFETDIGEDFDPKLSKMKK 300

Query: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360
           DVVVGVKDTRDVDNDFFLVVVKI DHQGPLST FPIENRN PVTMKALKNHLDRSKGLPF
Sbjct: 301 DVVVGVKDTRDVDNDFFLVVVKIFDHQGPLSTIFPIENRNAPVTMKALKNHLDRSKGLPF 360

Query: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411
           VKRISDFHLLL LARVLDVSSDVPALAECVQTQT VPEGYKILIESMASAA
Sbjct: 361 VKRISDFHLLLSLARVLDVSSDVPALAECVQTQTAVPEGYKILIESMASAA 411

BLAST of Cucsat.G21255 vs. NCBI nr
Match: XP_022155640.1 (NPL4-like protein 1 [Momordica charantia])

HSP 1 Score: 772 bits (1993), Expect = 1.28e-280
Identity = 387/411 (94.16%), Postives = 399/411 (97.08%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQ+KIP+HNQTLST+QNILLAK+ DDLSK
Sbjct: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQMKIPVHNQTLSTHQNILLAKSHDDLSK 60

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120
           FTDMSNPN+ LSSLNLSHGSIVFLAYEGERTVAGP  HPAGSFGRKMTMDDLIA+QMRIT
Sbjct: 61  FTDMSNPNSLLSSLNLSHGSIVFLAYEGERTVAGPAFHPAGSFGRKMTMDDLIARQMRIT 120

Query: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180
           RQENPHCELVSFDRDCANAFQ+YVNETLAFA+KRGG MYGTVSPEGKVEVDFIYEPPQQG
Sbjct: 121 RQENPHCELVSFDRDCANAFQNYVNETLAFAIKRGGFMYGTVSPEGKVEVDFIYEPPQQG 180

Query: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240
           TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAA+FHS+
Sbjct: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAEFHSD 240

Query: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300
           S LKEWVTAVVKLEVNEDGGADVHFEAFQMSD CIRLFKE WFETDIGEDFDPKLSKMKK
Sbjct: 241 SGLKEWVTAVVKLEVNEDGGADVHFEAFQMSDTCIRLFKEGWFETDIGEDFDPKLSKMKK 300

Query: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360
           DVVVGVKDTRDVDNDFFLVVVKI DHQGPLST+F IENRN PVTMKALKNHL+RSKGLPF
Sbjct: 301 DVVVGVKDTRDVDNDFFLVVVKIFDHQGPLSTSFAIENRNAPVTMKALKNHLERSKGLPF 360

Query: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411
           VKRISDFHLLLLLARVLDVSSDVPALAECVQTQT VPEGYKILIESMASAA
Sbjct: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTAVPEGYKILIESMASAA 411

BLAST of Cucsat.G21255 vs. NCBI nr
Match: XP_022958337.1 (NPL4-like protein 1 [Cucurbita moschata] >XP_023534714.1 NPL4-like protein 1 [Cucurbita pepo subsp. pepo] >KAG7035781.1 NPL4-like protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 760 bits (1962), Expect = 6.80e-276
Identity = 384/411 (93.43%), Postives = 395/411 (96.11%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERVAVE+PHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLA    D SK
Sbjct: 1   MMLRIRSRDGLERVAVESPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLANAHGDRSK 60

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120
           F DMSNPNT LSSLNLSHGSIV+LAYEGERTVAGP++HPAGSFGRKMTMDDLIA+QMRIT
Sbjct: 61  FMDMSNPNTLLSSLNLSHGSIVYLAYEGERTVAGPSIHPAGSFGRKMTMDDLIARQMRIT 120

Query: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180
           RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGG MYGTVS EGKVEVDFIYEPPQQG
Sbjct: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGFMYGTVSTEGKVEVDFIYEPPQQG 180

Query: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240
           TEDNLLFFRD DEERLVEAIAVGLGMRKVGFIFTQTIS+++KDYTLSNREVLQAA+FHSE
Sbjct: 181 TEDNLLFFRDQDEERLVEAIAVGLGMRKVGFIFTQTISRERKDYTLSNREVLQAAEFHSE 240

Query: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300
           S LKEWVTAVVKLEVNEDGGADVHFEAFQMSD+CI+LFKE WFETDI EDFDPKLSKMKK
Sbjct: 241 SGLKEWVTAVVKLEVNEDGGADVHFEAFQMSDVCIKLFKEGWFETDIQEDFDPKLSKMKK 300

Query: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360
           DVVVGVKDTRDVDNDFFLVVVKI DHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGL F
Sbjct: 301 DVVVGVKDTRDVDNDFFLVVVKIFDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLTF 360

Query: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411
           VKRISDFHLLLLLARVLDVSSDVPALAECVQTQT VPEGYKILIESMASAA
Sbjct: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTPVPEGYKILIESMASAA 411

BLAST of Cucsat.G21255 vs. ExPASy TrEMBL
Match: A0A0A0KJC3 (MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139050 PE=3 SV=1)

HSP 1 Score: 816 bits (2109), Expect = 1.30e-298
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK
Sbjct: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120
           FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT
Sbjct: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120

Query: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180
           RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG
Sbjct: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180

Query: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240
           TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE
Sbjct: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240

Query: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300
           SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK
Sbjct: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300

Query: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360
           DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF
Sbjct: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360

Query: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411
           VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA
Sbjct: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411

BLAST of Cucsat.G21255 vs. ExPASy TrEMBL
Match: A0A1S3AU81 (NPL4-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103483000 PE=3 SV=1)

HSP 1 Score: 805 bits (2079), Expect = 4.86e-294
Identity = 406/411 (98.78%), Postives = 407/411 (99.03%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLS NQNILLAKTQDDLSK
Sbjct: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSANQNILLAKTQDDLSK 60

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120
           FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT
Sbjct: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120

Query: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180
           RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG
Sbjct: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180

Query: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240
           TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE
Sbjct: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240

Query: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300
           SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKE WFETDIGEDFDPKLSKMKK
Sbjct: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKEGWFETDIGEDFDPKLSKMKK 300

Query: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360
           DVVVGVKDTRDVDNDFFLVVVKI DHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF
Sbjct: 301 DVVVGVKDTRDVDNDFFLVVVKIFDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360

Query: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411
           VKRISDFHLLLLL+RVLDVSSDVPALAECVQTQT VPEGYKILIESMASAA
Sbjct: 361 VKRISDFHLLLLLSRVLDVSSDVPALAECVQTQTAVPEGYKILIESMASAA 411

BLAST of Cucsat.G21255 vs. ExPASy TrEMBL
Match: A0A6J1DMZ8 (NPL4-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111022722 PE=3 SV=1)

HSP 1 Score: 772 bits (1993), Expect = 6.21e-281
Identity = 387/411 (94.16%), Postives = 399/411 (97.08%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQ+KIP+HNQTLST+QNILLAK+ DDLSK
Sbjct: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQMKIPVHNQTLSTHQNILLAKSHDDLSK 60

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120
           FTDMSNPN+ LSSLNLSHGSIVFLAYEGERTVAGP  HPAGSFGRKMTMDDLIA+QMRIT
Sbjct: 61  FTDMSNPNSLLSSLNLSHGSIVFLAYEGERTVAGPAFHPAGSFGRKMTMDDLIARQMRIT 120

Query: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180
           RQENPHCELVSFDRDCANAFQ+YVNETLAFA+KRGG MYGTVSPEGKVEVDFIYEPPQQG
Sbjct: 121 RQENPHCELVSFDRDCANAFQNYVNETLAFAIKRGGFMYGTVSPEGKVEVDFIYEPPQQG 180

Query: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240
           TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAA+FHS+
Sbjct: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAEFHSD 240

Query: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300
           S LKEWVTAVVKLEVNEDGGADVHFEAFQMSD CIRLFKE WFETDIGEDFDPKLSKMKK
Sbjct: 241 SGLKEWVTAVVKLEVNEDGGADVHFEAFQMSDTCIRLFKEGWFETDIGEDFDPKLSKMKK 300

Query: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360
           DVVVGVKDTRDVDNDFFLVVVKI DHQGPLST+F IENRN PVTMKALKNHL+RSKGLPF
Sbjct: 301 DVVVGVKDTRDVDNDFFLVVVKIFDHQGPLSTSFAIENRNAPVTMKALKNHLERSKGLPF 360

Query: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411
           VKRISDFHLLLLLARVLDVSSDVPALAECVQTQT VPEGYKILIESMASAA
Sbjct: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTAVPEGYKILIESMASAA 411

BLAST of Cucsat.G21255 vs. ExPASy TrEMBL
Match: A0A6J1H1K2 (NPL4-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111459587 PE=3 SV=1)

HSP 1 Score: 760 bits (1962), Expect = 3.29e-276
Identity = 384/411 (93.43%), Postives = 395/411 (96.11%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERVAVE+PHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLA    D SK
Sbjct: 1   MMLRIRSRDGLERVAVESPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLANAHGDRSK 60

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120
           F DMSNPNT LSSLNLSHGSIV+LAYEGERTVAGP++HPAGSFGRKMTMDDLIA+QMRIT
Sbjct: 61  FMDMSNPNTLLSSLNLSHGSIVYLAYEGERTVAGPSIHPAGSFGRKMTMDDLIARQMRIT 120

Query: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180
           RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGG MYGTVS EGKVEVDFIYEPPQQG
Sbjct: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGFMYGTVSTEGKVEVDFIYEPPQQG 180

Query: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240
           TEDNLLFFRD DEERLVEAIAVGLGMRKVGFIFTQTIS+++KDYTLSNREVLQAA+FHSE
Sbjct: 181 TEDNLLFFRDQDEERLVEAIAVGLGMRKVGFIFTQTISRERKDYTLSNREVLQAAEFHSE 240

Query: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300
           S LKEWVTAVVKLEVNEDGGADVHFEAFQMSD+CI+LFKE WFETDI EDFDPKLSKMKK
Sbjct: 241 SGLKEWVTAVVKLEVNEDGGADVHFEAFQMSDVCIKLFKEGWFETDIQEDFDPKLSKMKK 300

Query: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360
           DVVVGVKDTRDVDNDFFLVVVKI DHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGL F
Sbjct: 301 DVVVGVKDTRDVDNDFFLVVVKIFDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLTF 360

Query: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411
           VKRISDFHLLLLLARVLDVSSDVPALAECVQTQT VPEGYKILIESMASAA
Sbjct: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTPVPEGYKILIESMASAA 411

BLAST of Cucsat.G21255 vs. ExPASy TrEMBL
Match: A0A6J1K7Q3 (NPL4-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111490933 PE=3 SV=1)

HSP 1 Score: 756 bits (1952), Expect = 1.10e-274
Identity = 381/411 (92.70%), Postives = 394/411 (95.86%), Query Frame = 0

Query: 1   MMLRIRSRDGLERVAVENPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLAKTQDDLSK 60
           MMLRIRSRDGLERVAVE+PHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLA    D SK
Sbjct: 1   MMLRIRSRDGLERVAVESPHITIAQLKAIIQSQLKIPIHNQTLSTNQNILLANAHGDRSK 60

Query: 61  FTDMSNPNTYLSSLNLSHGSIVFLAYEGERTVAGPTVHPAGSFGRKMTMDDLIAKQMRIT 120
           F DMSNPNT LSSLNLSHGSIV+LAYEGERTVAGP++HPAGSFGRKMTMDDL+A+QMRIT
Sbjct: 61  FMDMSNPNTLLSSLNLSHGSIVYLAYEGERTVAGPSIHPAGSFGRKMTMDDLVARQMRIT 120

Query: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGMMYGTVSPEGKVEVDFIYEPPQQG 180
           RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGG MYGTVS EGKVEVDFIYEPPQQG
Sbjct: 121 RQENPHCELVSFDRDCANAFQHYVNETLAFAVKRGGFMYGTVSTEGKVEVDFIYEPPQQG 180

Query: 181 TEDNLLFFRDHDEERLVEAIAVGLGMRKVGFIFTQTISQDKKDYTLSNREVLQAAQFHSE 240
           TEDNLLFFRD DEERLVEAIAVGLGMRKVGFIFTQTIS+++KDYTLSNREVLQAA+FHSE
Sbjct: 181 TEDNLLFFRDQDEERLVEAIAVGLGMRKVGFIFTQTISRERKDYTLSNREVLQAAEFHSE 240

Query: 241 SELKEWVTAVVKLEVNEDGGADVHFEAFQMSDMCIRLFKECWFETDIGEDFDPKLSKMKK 300
           + LKEWVTAVVKLEVNEDGGADVHFEAFQMSD+CI+LFKE WFETDI EDFDPKLSKMKK
Sbjct: 241 TGLKEWVTAVVKLEVNEDGGADVHFEAFQMSDVCIKLFKEGWFETDIQEDFDPKLSKMKK 300

Query: 301 DVVVGVKDTRDVDNDFFLVVVKILDHQGPLSTTFPIENRNVPVTMKALKNHLDRSKGLPF 360
           DVVVGVKDTRDVDNDFFLVVVKI DHQGPLSTTFPIENRNVPVTMKALKNHLDR KGL F
Sbjct: 301 DVVVGVKDTRDVDNDFFLVVVKIFDHQGPLSTTFPIENRNVPVTMKALKNHLDRLKGLTF 360

Query: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTGVPEGYKILIESMASAA 411
           VKRISDFHLLLLLARVLDVSSDVPALAECVQTQT VPEGYKILIESMASAA
Sbjct: 361 VKRISDFHLLLLLARVLDVSSDVPALAECVQTQTPVPEGYKILIESMASAA 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYC24.0e-18174.21NPL4-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At3g63000 PE=1 SV=1[more]
O822645.4e-17875.12NPL4-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At2g47970 PE=1 SV=1[more]
Q9AS332.8e-12657.04NPL4-like protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0377700 PE=2 ... [more]
Q8TAT61.5e-1828.10Nuclear protein localization protein 4 homolog OS=Homo sapiens OX=9606 GN=NPLOC4... [more]
P606701.9e-1828.10Nuclear protein localization protein 4 homolog OS=Mus musculus OX=10090 GN=Nploc... [more]
Match NameE-valueIdentityDescription
XP_004145952.12.68e-298100.00NPL4-like protein 1 [Cucumis sativus] >KGN49825.1 hypothetical protein Csa_00019... [more]
XP_008437660.11.00e-29398.78PREDICTED: NPL4-like protein 1 [Cucumis melo][more]
XP_038874364.12.17e-28897.08NPL4-like protein 1 [Benincasa hispida][more]
XP_022155640.11.28e-28094.16NPL4-like protein 1 [Momordica charantia][more]
XP_022958337.16.80e-27693.43NPL4-like protein 1 [Cucurbita moschata] >XP_023534714.1 NPL4-like protein 1 [Cu... [more]
Match NameE-valueIdentityDescription
A0A0A0KJC31.30e-298100.00MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139050 PE=3 SV... [more]
A0A1S3AU814.86e-29498.78NPL4-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103483000 PE=3 SV=1[more]
A0A6J1DMZ86.21e-28194.16NPL4-like protein 1 OS=Momordica charantia OX=3673 GN=LOC111022722 PE=3 SV=1[more]
A0A6J1H1K23.29e-27693.43NPL4-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111459587 PE=3 SV=1[more]
A0A6J1K7Q31.10e-27492.70NPL4-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111490933 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (B10) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024682Nuclear pore localisation protein Npl4, ubiquitin-like domainPFAMPF11543UN_NPL4coord: 1..85
e-value: 5.3E-6
score: 26.8
IPR007717Nuclear pore localisation protein NPL4, C-terminalPFAMPF05021NPL4coord: 167..279
e-value: 1.6E-12
score: 47.4
IPR007717Nuclear pore localisation protein NPL4, C-terminalCDDcd08061MPN_NPL4coord: 118..321
e-value: 6.04151E-60
score: 191.803
NoneNo IPR availableGENE3D3.10.20.90coord: 1..99
e-value: 1.8E-42
score: 145.4
NoneNo IPR availableCDDcd17055Ubl_AtNPL4_likecoord: 1..84
e-value: 3.16847E-23
score: 89.1962
IPR016563Nuclear protein localization protein 4PANTHERPTHR12710NUCLEAR PROTEIN LOCALIZATION 4coord: 2..90
coord: 98..327
IPR037518MPN domainPROSITEPS50249MPNcoord: 129..270
score: 17.545273
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 1..88

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsat.G21255.T1Cucsat.G21255.T1mRNA
Cucsat.G21255.T2Cucsat.G21255.T2mRNA
Cucsat.G21255.T4Cucsat.G21255.T4mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0043130 ubiquitin binding
molecular_function GO:0031625 ubiquitin protein ligase binding