Cucsat.G13224 (gene) Cucumber (B10) v3

BLAST of Cucsat.G13224 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 252.7 bits (644), Expect = 1.7e-65
Identity = 174/508 (34.25%), Postives = 269/508 (52.95%), Query Frame = 0

Query: 179 RREAILSNVDSIFGRDKIQESIIKELVNDEQKSPR--ILSIQGDGGMGKTALAKLVYNAD 238
           R+   +     ++GR+K ++ I+K L+N+   S    +L I G GG+GKT LA++V+N  
Sbjct: 140 RQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPILGMGGLGKTTLAQMVFNDQ 199

Query: 239 EVFDHFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKIL 298
            + +HF+ ++WVCVS+DFD +R+++ ++ S  G+++  + L  + L+ +LQ    GK+  
Sbjct: 200 RITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDL--APLQKKLQELLNGKRYF 259

Query: 299 LVLDDFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATT---HKIDKLDETISM 358
           LVLDD  N D E+   L+ ++K+G  G+ I+ITTR ++  ++  T   +++  L +    
Sbjct: 260 LVLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLEKIGSIMGTLQLYQLSNLSQEDCW 319

Query: 359 QIFEDTYGSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLS 418
            +F+            +     K +V +CGG PLA K L GLL  K  + ++  +V+D S
Sbjct: 320 LLFKQRAFCHQTETSPKLMEIGKEIVKKCGGVPLAAKTLGGLLRFKREE-SEWEHVRD-S 379

Query: 419 EKWKQEEANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGIL 478
           E W   +  N   VL ALRLSY  +P  L+ CF   +V PKD       LI LWMA   L
Sbjct: 380 EIWNLPQDEN--SVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIEKEYLIALWMAHSFL 439

Query: 479 PSGTKDNPEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNF 538
            S      E+VG + + E   R    ++E  +   +FK+H L+HDLA    +   +  + 
Sbjct: 440 LSKGNMELEDVGNEVWNELYLRSFFQEIEVKSGKTYFKMHDLIHDLATSMFSASASSRSI 499

Query: 539 HMLSFVDC-DSIPSSTNYDNTRFISI-PVVGGAGPNINSDLFKCITQFRQLRFLYLCNSS 598
             ++  D  D +   TNY +   I    VV    P+    LFK   +F  LR L L NS 
Sbjct: 500 RQINVKDDEDMMFIVTNYKDMMSIGFSEVVSSYSPS----LFK---RFVSLRVLNLSNSE 559

Query: 599 LEEIPTSIDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLI 658
            E++P+S+  L HLR LDL G+ ++  LP+ +CKLQ+LQTL L  C  L  LP+    L 
Sbjct: 560 FEQLPSSVGDLVHLRYLDLSGN-KICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLC 619

Query: 659 SLRFLWIQTKQARLEKDEIGSLTSLRFL 680
           SLR L +           IG LT L+ L
Sbjct: 620 SLRNLVLDHCPLTSMPPRIGLLTCLKTL 633

BLAST of Cucsat.G13224 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 251.1 bits (640), Expect = 4.9e-65
Identity = 236/831 (28.40%), Postives = 380/831 (45.73%), Query Frame = 0

Query: 4   LLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELNELL 63
           +LD++   L G ++     L F   Q E + L +    ++A L DA+EKQ ++  L   L
Sbjct: 9   VLDNLTSFLKGELV-----LLF-GFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWL 68

Query: 64  KNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGSKFQ 123
           + L       D  ++E +    ++ +Q+       K             + PF    K  
Sbjct: 69  QKLNAATYEVDDILDEYKTKATRF-LQSEYGRYHPK-------------VIPFR--HKVG 128

Query: 124 EDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRREAI 183
           + +  +  +L +I +   KF L E   E                      R    R    
Sbjct: 129 KRMDQVMKKLNAIAEERKKFHLQEKIIE----------------------RQAATRETGS 188

Query: 184 LSNVDSIFGRDKIQESIIKELVN---DEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 243
           +     ++GRDK ++ I+K L+N   D QK   +L I G GG+GKT L+++V+N   V +
Sbjct: 189 VLTEPQVYGRDKEKDEIVKILINTASDAQKL-SVLPILGMGGLGKTTLSQMVFNDQRVTE 248

Query: 244 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 303
            F  ++W+C+S+DF+ +R+++ ++ S  G++++ + L  + L+ +LQ    GK+  LVLD
Sbjct: 249 RFYPKIWICISDDFNEKRLIKAIVESIEGKSLSDMDL--APLQKKLQELLNGKRYFLVLD 308

Query: 304 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATT---HKIDKLDETISMQIFE 363
           D  N D  + + L+ ++K+G  G+ ++ TTR ++  ++  T   +++  L       +F 
Sbjct: 309 DVWNEDQHKWANLRAVLKVGASGAFVLTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFM 368

Query: 364 DTYGSEGLSEGLRDDLYL--KNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEK 423
               + G  E +  +L    K +V +CGG PLA K L G+L  K  +  +  +V+D S  
Sbjct: 369 QR--AFGHQEEINPNLMAIGKEIVKKCGGVPLAAKTLGGILRFKREE-REWEHVRD-SPI 428

Query: 424 WK--QEEANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGIL 483
           W   Q+E++    +L ALRLSY  +P  L+ CF+  +V PKD       LI  WMA G L
Sbjct: 429 WNLPQDESS----ILPALRLSYHHLPLDLRQCFVYCAVFPKDTKMAKENLIAFWMAHGFL 488

Query: 484 PSGTKDNPEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNF 543
            S      E+VG + + E   R    ++E  +   +FK+H L+HDLA    +   +  N 
Sbjct: 489 LSKGNLELEDVGNEVWNELYLRSFFQEIEVESGKTYFKMHDLIHDLATSLFSANTSSSNI 548

Query: 544 HMLSFVDCDSIPSSTNYDNTRFISIPVVGGAGPNINSDLFKCITQFRQLRFLYLCNSSLE 603
             +          + NYD    +SI    G    ++S     + +F  LR L L NS+L 
Sbjct: 549 REI----------NANYDG-YMMSI----GFAEVVSSYSPSLLQKFVSLRVLNLRNSNLN 608

Query: 604 EIPTSIDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISL 663
           ++P+SI  L HLR LDL G+ R++ LP+ +CKLQ+LQTL L +C  L  LP+    L SL
Sbjct: 609 QLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSL 668

Query: 664 RFLWIQTKQARLEKDEIGSLTSLRFLA---IGRSENLTHLFEDINKLNSLKTLIIYECKS 723
           R L +           IG LT L+ L+   IG+ +         ++L  LK L +Y   S
Sbjct: 669 RNLLLDGCSLTSTPPRIGLLTCLKSLSCFVIGKRKG--------HQLGELKNLNLYGSIS 728

Query: 724 LLTLPK-------------GLENMKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAV 783
           +  L +                N+ S+C    W+ D                +L  L   
Sbjct: 729 ITKLDRVKKDTDAKEANLSAKANLHSLCLS--WDLD--------GKHRYDSEVLEALKPH 739

Query: 784 STLPNWLSNLDGTLEVLEIGEFPTLRKLPIW-----LLNFWELRILGISNC 804
           S            L+ LEI  F  +R LP W     L N   +RI G  NC
Sbjct: 789 S-----------NLKYLEINGFGGIR-LPDWMNQSVLKNVVSIRIRGCENC 739

BLAST of Cucsat.G13224 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.6e-63
Identity = 211/699 (30.19%), Postives = 339/699 (48.50%), Query Frame = 0

Query: 4   LLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELNELL 63
           LLD++   L G ++     L F   Q E + L +    ++A L DA+EKQ +N  L   L
Sbjct: 9   LLDNLTSFLKGELV-----LLF-GFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWL 68

Query: 64  KNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNF--LISPFNTGSK 123
           + L       D  ++E +                 K  +FS      +   + PF     
Sbjct: 69  QKLNAATYEVDDILDEYK----------------TKATRFSQSEYGRYHPKVIPFR---- 128

Query: 124 FQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRRE 183
                         + K M         D+ +KKLK     R +            +RRE
Sbjct: 129 ------------HKVGKRM---------DQVMKKLKAIAEERKNFHLHEKIVERQAVRRE 188

Query: 184 --AILSNVDSIFGRDKIQESIIKELVND--EQKSPRILSIQGDGGMGKTALAKLVYNADE 243
             ++L+    ++GRDK ++ I+K L+N+  + +   +L I G GG+GKT LA++V+N   
Sbjct: 189 TGSVLTE-PQVYGRDKEKDEIVKILINNVSDAQHLSVLPILGMGGLGKTTLAQMVFNDQR 248

Query: 244 VFDHFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILL 303
           V +HF  ++W+CVSEDFD +R+++ ++ S  G  +    +  + L+ +LQ    GK+ LL
Sbjct: 249 VTEHFHSKIWICVSEDFDEKRLIKAIVESIEGRPLLG-EMDLAPLQKKLQELLNGKRYLL 308

Query: 304 VLDDFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATT---HKIDKLDETISMQ 363
           VLDD  N D ++ + L+ ++K+G  G+ ++ TTR ++  ++  T   +++  L +     
Sbjct: 309 VLDDVWNEDQQKWANLRAVLKVGASGASVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWL 368

Query: 364 IFEDTYGSEGLSEGLRDDLYL--KNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDL 423
           +F     + G  E +  +L    K +V + GG PLA K L G+L  K  + A   +V+D 
Sbjct: 369 LFMQR--AFGHQEEINPNLVAIGKEIVKKSGGVPLAAKTLGGILCFKREERAWE-HVRD- 428

Query: 424 SEKWK--QEEANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQ 483
           S  W   Q+E++    +L ALRLSY  +P  LK CF   +V PKD      +LI LWMA 
Sbjct: 429 SPIWNLPQDESS----ILPALRLSYHQLPLDLKQCFAYCAVFPKDAKMEKEKLISLWMAH 488

Query: 484 GILPSGTKDNPEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNL 543
           G L S      E+VG++ +KE   R    ++E      +FK+H L+HDLA    +   + 
Sbjct: 489 GFLLSKGNMELEDVGDEVWKELYLRSFFQEIEVKDGKTYFKMHDLIHDLATSLFSANTSS 548

Query: 544 GNFHMLSFVDCDSIPSSTNYDNTRFISIPVVGGAGPNINSDLFKCITQFRQLRFLYLCNS 603
            N   ++            +  T  +SI    G    +       + +F  LR L L +S
Sbjct: 549 SNIREIN-----------KHSYTHMMSI----GFAEVVFFYTLPPLEKFISLRVLNLGDS 608

Query: 604 SLEEIPTSIDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNL 663
           +  ++P+SI  L HLR L+L GS  ++ LP+ +CKLQ+LQTL L +C++L  LP+    L
Sbjct: 609 TFNKLPSSIGDLVHLRYLNLYGS-GMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKL 634

Query: 664 ISLRFLWIQTKQA-RLEKDEIGSLTSLRFL---AIGRSE 686
            SLR L +   Q+       IGSLT L+ L    +GR +
Sbjct: 669 GSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKK 634

BLAST of Cucsat.G13224 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 4.6e-63
Identity = 196/661 (29.65%), Postives = 311/661 (47.05%), Query Frame = 0

Query: 29  QSELKNLETDVLNLKARLRDAEEKQASNCELNELLKNLKNVFSRADIAIEELECDYLKWR 88
           + E + L +    ++A L+DA+EKQ  +  +   L+ L +     D  + E +       
Sbjct: 28  EKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSAAYEVDDILGECK------- 87

Query: 89  VQNRKNDVDDKGCQFSSCFSSNFLISPFNTGSKFQEDLKIITSELRSIEKAMSKFSLVED 148
                    ++  +F       +     N   K    +K I  +L +I +   KF  +E 
Sbjct: 88  ---------NEAIRFEQSRLGFYHPGIINFRHKIGRRMKEIMEKLDAISEERRKFHFLE- 147

Query: 149 EDEYIKKLKGEMTLRTSITGSHAFARLLRLRREAILSNVDSIFGRDKIQESIIKELVNDE 208
                            IT   A A     R    +     ++GRDK ++ I+K L+N+ 
Sbjct: 148 ----------------KITERQAAA---ATRETGFVLTEPKVYGRDKEEDEIVKILINNV 207

Query: 209 QKSPR--ILSIQGDGGMGKTALAKLVYNADEVFDHFDKRMWVCVSEDFDIRRILREVL-- 268
             +    +  I G GG+GKT LA++++N + V  HF+ ++WVCVS+DFD +R+++ ++  
Sbjct: 208 NVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGN 267

Query: 269 MSATGENVTTVALTESRLRIRLQRYFFGKKILLVLDDFGNLDPERVSELKKIVKMGVGGS 328
           +  +  +V  +A  +     +LQ    GK+ LLVLDD  N D E+ ++L+ ++ +G  G+
Sbjct: 268 IERSSPHVEDLASFQK----KLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGA 327

Query: 329 KIMITTRSDETLNVATT---HKIDKLDETISMQIFEDTYGSEGLSEGLRDDLYLKNLVAE 388
            I+ TTR ++  ++  T   + +  L    S+ +F      +            K +V +
Sbjct: 328 SILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKK 387

Query: 389 CGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWK--QEEANNGGGVLCALRLSYDLMP 448
           CGG PLA K L GLL  K  + ++  +V+D +E W   Q+E++    +L ALRLSY  +P
Sbjct: 388 CGGVPLAAKTLGGLLRFKREE-SEWEHVRD-NEIWSLPQDESS----ILPALRLSYHHLP 447

Query: 449 SYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDNPEEVGEKYFKEFRDRRLLV 508
             L+ CF   +V PKD       LI LWMA G L S      E+VG + + E   R    
Sbjct: 448 LDLRQCFAYCAVFPKDTKMIKENLITLWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQ 507

Query: 509 DVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVDCDSIPSSTNYDNTRFISI- 568
           ++E  +   +FKIH L+HDLA    +   + GN   ++  D         Y +T  I   
Sbjct: 508 EIEAKSGNTYFKIHDLIHDLATSLFSASASCGNIREINVKD---------YKHTVSIGFA 567

Query: 569 PVVGGAGPNINSDLFKCITQFRQLRFLYLCNSSLEEIPTSIDTLKHLRCLDLRGSQRLKR 628
            VV    P++       + +F  LR L L  S LE++P+SI  L HLR LDL      + 
Sbjct: 568 AVVSSYSPSL-------LKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDL-SCNNFRS 625

Query: 629 LPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWIQTKQARLEKDEIGSLTSLRF 680
           LPE +CKLQ+LQTL +  C  L  LP+    L SLR L +           IG LT L+ 
Sbjct: 628 LPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPRIGLLTCLKT 625

BLAST of Cucsat.G13224 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 2.0e-50
Identity = 187/693 (26.98%), Postives = 320/693 (46.18%), Query Frame = 0

Query: 32  LKNLETDVLNLKARLRDAEEKQASNCELNELLKNLKNVFSRADIAIEELECDYLKWRVQN 91
           L+ L T +L + A L DAEEKQ +N  + + +  L++V   A+ A++++  + L+  +  
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNI-- 98

Query: 92  RKNDVDDKGCQFSSCFSSNFLISPFNTGSKFQEDLKIITSELRSIEKAMSKFSLVEDEDE 151
                   G + SS                        ++ LR +   MS    ++   E
Sbjct: 99  --------GAESSS------------------------SNRLRQLRGRMSLGDFLDGNSE 158

Query: 152 YIKKLKGEMTLR-------TSITGSHAFARLLRLRREAILSNVD--SIFGRDKIQESIIK 211
           +++    ++T+R        +I G      ++  +R    S VD   +FGRD  ++ I++
Sbjct: 159 HLETRLEKVTIRLERLASQRNILGLKELTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMR 218

Query: 212 ELV--NDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFDHFDKRMWVCVSEDFDIRRIL 271
            L+  N +     +++I G GG+GKT L++L+YN   V  +F  ++W  VSE+FD+ +I 
Sbjct: 219 FLIPENGKDNGITVVAIVGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKIT 278

Query: 272 REVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLDDFGNLDPERVSELKKIVKMG 331
           ++V  S T        L   +++++ +    G   LLVLDD  N +      L++     
Sbjct: 279 KKVYESVTSRPCEFTDLDVLQVKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHA 338

Query: 332 VGGSKIMITTRSDETLNV---ATTHKIDKLDETISMQIFEDT-YGSEGLSEGLRDDLYLK 391
             GS+I++TTRS    ++      H +  L +     +F  T +G++            +
Sbjct: 339 AQGSQILVTTRSQRVASIMCAVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAE 398

Query: 392 NLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEEANNGGGVLCALRLSYD 451
            +V +C G PLA+K L G+L     +G      + LS +     A+    +L  LR+SY 
Sbjct: 399 RIVHKCRGLPLAVKTLGGVLR---FEGKVIEWERVLSSRIWDLPADK-SNLLPVLRVSYY 458

Query: 452 LMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGIL-PSGTKDNPEEVGEKYFKEFRDR 511
            +P++LK CF   S+ PK + F   +++ LWMA+G L  + +  N EE+G +YF E   R
Sbjct: 459 YLPAHLKRCFAYCSIFPKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESR 518

Query: 512 RLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVDCDSIPSSTNYDNTRF 571
            LL   +       + +H  +++LA      Q   G F    F D   +  S   + TR+
Sbjct: 519 SLLQKTKTR-----YIMHDFINELA------QFASGEFSS-KFEDGCKLQVS---ERTRY 578

Query: 572 ISIPVVGGAGPN-------------------INSDLFKCITQ---------FRQLRFLYL 631
           +S      A P                     NS    C+ Q           +LR L L
Sbjct: 579 LSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSL 638

Query: 632 CNSSLEEIPTS-IDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRN 680
            +  +  +P      + H R LDL  ++ L++LP+S+C + +LQTL+L++CS L+ELP +
Sbjct: 639 SHYKIARLPPDFFKNISHARFLDLSRTE-LEKLPKSLCYMYNLQTLLLSYCSSLKELPTD 677

BLAST of Cucsat.G13224 vs. NCBI nr
Match: XP_004136788.1 (putative disease resistance protein RGA3 [Cucumis sativus] >KAE8651193.1 hypothetical protein Csa_001059 [Cucumis sativus])

HSP 1 Score: 1898 bits (4917), Expect = 0.0
Identity = 958/958 (100.00%), Postives = 958/958 (100.00%), Query Frame = 0

Query: 1   MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN 60
           MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN
Sbjct: 1   MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN 60

Query: 61  ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS 120
           ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS
Sbjct: 61  ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS 120

Query: 121 KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR 180
           KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR
Sbjct: 121 KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR 180

Query: 181 EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240
           EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD
Sbjct: 181 EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240

Query: 241 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300
           HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD
Sbjct: 241 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300

Query: 301 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY 360
           DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY
Sbjct: 301 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY 360

Query: 361 GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE 420
           GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE
Sbjct: 361 GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE 420

Query: 421 ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN 480
           ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN
Sbjct: 421 ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN 480

Query: 481 PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD 540
           PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD
Sbjct: 481 PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD 540

Query: 541 CDSIPSSTNYDNTRFISIPVVGGAGPNINSDLFKCITQFRQLRFLYLCNSSLEEIPTSID 600
           CDSIPSSTNYDNTRFISIPVVGGAGPNINSDLFKCITQFRQLRFLYLCNSSLEEIPTSID
Sbjct: 541 CDSIPSSTNYDNTRFISIPVVGGAGPNINSDLFKCITQFRQLRFLYLCNSSLEEIPTSID 600

Query: 601 TLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWIQT 660
           TLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWIQT
Sbjct: 601 TLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWIQT 660

Query: 661 KQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGLEN 720
           KQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGLEN
Sbjct: 661 KQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGLEN 720

Query: 721 MKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGEFP 780
           MKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGEFP
Sbjct: 721 MKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGEFP 780

Query: 781 TLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLLKK 840
           TLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLLKK
Sbjct: 781 TLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLLKK 840

Query: 841 SMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNNASPPGTEQPSKT 900
           SMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNNASPPGTEQPSKT
Sbjct: 841 SMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNNASPPGTEQPSKT 900

Query: 901 KHDDANNNMSHPGIGLLSESKQEHTNNNINEIETVKVCLGDNDHAEAHQAMVTTYEGF 958
           KHDDANNNMSHPGIGLLSESKQEHTNNNINEIETVKVCLGDNDHAEAHQAMVTTYEGF
Sbjct: 901 KHDDANNNMSHPGIGLLSESKQEHTNNNINEIETVKVCLGDNDHAEAHQAMVTTYEGF 958

BLAST of Cucsat.G13224 vs. NCBI nr
Match: KAA0053831.1 (putative disease resistance protein RGA3 isoform X3 [Cucumis melo var. makuwa] >TYK25570.1 putative disease resistance protein RGA3 isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 1515 bits (3922), Expect = 0.0
Identity = 789/982 (80.35%), Postives = 859/982 (87.47%), Query Frame = 0

Query: 1   MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN 60
           MVGLL SVAGNLLGRIIEAADR EFRAI+ ELKNLET VL+LKARLRDAEEKQAS+ EL 
Sbjct: 1   MVGLLGSVAGNLLGRIIEAADRREFRAIRRELKNLETAVLDLKARLRDAEEKQASDPELY 60

Query: 61  ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS 120
           + L+ LK+ FS AD  IEELECDYLKWRVQNRKNDVDDKG QFSSCFSSNF IS  NT +
Sbjct: 61  DQLRKLKHAFSMADSVIEELECDYLKWRVQNRKNDVDDKGYQFSSCFSSNFPISSCNTVA 120

Query: 121 KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR 180
           KFQ+    +T ELR IE+ MSKFSLVEDEDEYIK LKGEMTLRTSITGS AFARLLRLR+
Sbjct: 121 KFQQ----LTEELRLIEETMSKFSLVEDEDEYIKNLKGEMTLRTSITGSQAFARLLRLRK 180

Query: 181 EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240
           EAILSNVDSIFGRDK+QESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD
Sbjct: 181 EAILSNVDSIFGRDKVQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240

Query: 241 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300
           HFDKR+WVCVSEDFDI+RI++EVL+SATGENVTTVALTESRLRIRLQRYFFGKKILLVLD
Sbjct: 241 HFDKRIWVCVSEDFDIQRIIKEVLISATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300

Query: 301 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY 360
           DFGNLD +RVSELK+IVKMGV GSKIMITTRS +T NVA THKIDKLD+TISMQIFEDT+
Sbjct: 301 DFGNLDHDRVSELKEIVKMGVDGSKIMITTRSHKTPNVAATHKIDKLDKTISMQIFEDTF 360

Query: 361 GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE 420
           G   LS    +D+ LKNLVAECGGAPLAIKCLAGLLSSKPSDGA SPNVKDLSEKWK EE
Sbjct: 361 GGNELS----NDVDLKNLVAECGGAPLAIKCLAGLLSSKPSDGANSPNVKDLSEKWKLEE 420

Query: 421 ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN 480
           AN GGGV CALRLSYDLMPSYLKP FLCFS+LP+DNVFFSFELIQLW+ QG LPSGTKD+
Sbjct: 421 ANYGGGVSCALRLSYDLMPSYLKPFFLCFSLLPRDNVFFSFELIQLWLEQGFLPSGTKDD 480

Query: 481 PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD 540
           PEE+GEKYFKE  DRRLLVDVEEHTLGYWFKIH+LVHDLAVQKA  QKNLG FH LSFVD
Sbjct: 481 PEEIGEKYFKELWDRRLLVDVEEHTLGYWFKIHNLVHDLAVQKAEGQKNLGIFHQLSFVD 540

Query: 541 CDS-IPSSTNYDNTRFISIPVVGGAGPNINSD-LFKCITQFRQLRFLYLCNSSLEEIPTS 600
           C S IP ST+Y++  F+SIPVVGGA P IN D LFKCIT+F+QLRFLYLCNSSLEEIPTS
Sbjct: 541 CKSNIPPSTSYNDIHFLSIPVVGGAEPKINGDPLFKCITKFKQLRFLYLCNSSLEEIPTS 600

Query: 601 IDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWI 660
           I TLKHLRCLDLRGSQRLKRLPESICKLQSL+TL+LAFCSELEELPRNIKNLISLRFLW+
Sbjct: 601 IGTLKHLRCLDLRGSQRLKRLPESICKLQSLRTLILAFCSELEELPRNIKNLISLRFLWV 660

Query: 661 QTKQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGL 720
           QTKQA L KD+IGSLTSLRFLAIGRS+NLTHLFEDI+KLN LKTLIIY+CKSLLTLPKGL
Sbjct: 661 QTKQASLGKDQIGSLTSLRFLAIGRSDNLTHLFEDIDKLNFLKTLIIYDCKSLLTLPKGL 720

Query: 721 ENMKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGE 780
           EN++S+CNMGIW C+RLRFTFSLASL+LKKLILR+LTAVS+LP WL++LD TLEVLEIGE
Sbjct: 721 ENVESLCNMGIWGCERLRFTFSLASLNLKKLILRQLTAVSSLPKWLAHLDDTLEVLEIGE 780

Query: 781 FPTLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLL 840
           FPTLR+LP+W  N+WELRILGISNCP+L H+ F  EL  + DKIEELR+TFCGS SKS  
Sbjct: 781 FPTLRELPMWFSNYWELRILGISNCPQLNHECFHHELPIYRDKIEELRVTFCGSSSKSSW 840

Query: 841 KKSMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNN---------- 900
           KKSM+EI+ ++  I YI  IYVDSKR+ P  EST++PKEAETKQDDA NN          
Sbjct: 841 KKSMEEIKNKNPNISYIRAIYVDSKRIMPLEESTEKPKEAETKQDDANNNMSHVDIGLLS 900

Query: 901 ----------ASPPGTEQPSKTKHDDANNNMSHPGIGLLSESKQEHTN--NNINEIETVK 958
                      S PGT+QPS  KHDDANNN SH GIGL SE+KQEH N  +N+NE ET K
Sbjct: 901 KIEQAHANNNVSHPGTKQPSMNKHDDANNNTSHSGIGLFSETKQEHANKNDNVNENETGK 960

BLAST of Cucsat.G13224 vs. NCBI nr
Match: XP_008443319.1 (PREDICTED: putative disease resistance protein RGA4 isoform X1 [Cucumis melo] >XP_008443320.1 PREDICTED: putative disease resistance protein RGA4 isoform X2 [Cucumis melo])

HSP 1 Score: 1491 bits (3861), Expect = 0.0
Identity = 775/962 (80.56%), Postives = 844/962 (87.73%), Query Frame = 0

Query: 1   MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN 60
           MVGLL SVAGNLLGRIIEAADR EFRAI+ ELKNLET VL+LKARLRDAEEKQAS+ EL 
Sbjct: 1   MVGLLGSVAGNLLGRIIEAADRREFRAIRRELKNLETAVLDLKARLRDAEEKQASDPELY 60

Query: 61  ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS 120
           + L+ LK+ FS AD  IEELECDYLKWRVQNRKNDVDDKG QFSSCFSSNF IS  NT +
Sbjct: 61  DQLRKLKHAFSMADSVIEELECDYLKWRVQNRKNDVDDKGYQFSSCFSSNFPISSCNTVA 120

Query: 121 KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR 180
           KFQ+    +T ELR IE+ MSKFSLVEDEDEYIK LKGEMTLRTSITGS AFARLLRLR+
Sbjct: 121 KFQQ----LTEELRLIEETMSKFSLVEDEDEYIKNLKGEMTLRTSITGSQAFARLLRLRK 180

Query: 181 EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240
           EAILSNVDSIFGRDK+QESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD
Sbjct: 181 EAILSNVDSIFGRDKVQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240

Query: 241 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300
           HFDKR+WVCVSEDFDI+RI++EVL+SATGENVTTVALTESRLRIRLQRYFFGKKILLVLD
Sbjct: 241 HFDKRIWVCVSEDFDIQRIIKEVLISATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300

Query: 301 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY 360
           DFGNLD +RVSELK+IVKMGV GSKIMITTRS +T NVA THKIDKLD+TISMQIFEDT+
Sbjct: 301 DFGNLDHDRVSELKEIVKMGVDGSKIMITTRSHKTPNVAATHKIDKLDKTISMQIFEDTF 360

Query: 361 GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE 420
           G   LS    +D+ LKNLVAECGGAPLAIKCLAGLLSSKPSDGA SPNVKDLSEKWK EE
Sbjct: 361 GGNELS----NDVDLKNLVAECGGAPLAIKCLAGLLSSKPSDGANSPNVKDLSEKWKLEE 420

Query: 421 ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN 480
           AN GGGV CALRLSYDLMPSYLKP FLCFS+LP+DNVFFSFELIQLW+ QG LPSGTKD+
Sbjct: 421 ANYGGGVSCALRLSYDLMPSYLKPFFLCFSLLPRDNVFFSFELIQLWLEQGFLPSGTKDD 480

Query: 481 PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD 540
           PEE+GEKYFKE  DRRLLVDVEEHTLGYWFKIH+LVHDLAVQKA  QKNLG FH LSFVD
Sbjct: 481 PEEIGEKYFKELWDRRLLVDVEEHTLGYWFKIHNLVHDLAVQKAEGQKNLGIFHQLSFVD 540

Query: 541 CDS-IPSSTNYDNTRFISIPVVGGAGPNINSD-LFKCITQFRQLRFLYLCNSSLEEIPTS 600
           C S IP ST+Y++  F+SIPVVGGA P IN D LFKCIT+F+QLRFLYLCNSSLEEIPTS
Sbjct: 541 CKSNIPPSTSYNDIHFLSIPVVGGAEPKINGDPLFKCITKFKQLRFLYLCNSSLEEIPTS 600

Query: 601 IDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWI 660
           I TLKHLRCLDLRGSQRLKRLPESICKLQSL+TL+LAFCSELEELPRNIKNLISLRFLW+
Sbjct: 601 IGTLKHLRCLDLRGSQRLKRLPESICKLQSLRTLILAFCSELEELPRNIKNLISLRFLWV 660

Query: 661 QTKQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGL 720
           QTKQA L KD+IGSLTSLRFLAIGRS+NLTHLFEDI+KLN LKTLIIY+CKSLLTLPKGL
Sbjct: 661 QTKQASLGKDQIGSLTSLRFLAIGRSDNLTHLFEDIDKLNFLKTLIIYDCKSLLTLPKGL 720

Query: 721 ENMKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGE 780
           EN++S+CNMGIW C+RLRFTFSLASL+LKKLILR+LTAVS+LP WL++LD TLEVLEIGE
Sbjct: 721 ENVESLCNMGIWGCERLRFTFSLASLNLKKLILRQLTAVSSLPKWLAHLDDTLEVLEIGE 780

Query: 781 FPTLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLL 840
           FPTLR+LP+W  N+WELRILGISNCP+L H+ F  EL  + DKIEELR+TFCGS SKS  
Sbjct: 781 FPTLRELPMWFSNYWELRILGISNCPQLNHECFHHELPIYRDKIEELRVTFCGSSSKSSW 840

Query: 841 KKSMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNNASPPGTEQPS 900
           KKSM+EI+ ++  I YI  IYVDSKR+ P  EST++PKEAET                  
Sbjct: 841 KKSMEEIKNKNPNISYIRAIYVDSKRIMPLEESTEKPKEAET------------------ 900

Query: 901 KTKHDDANNNMSHPGIGLLSESKQEHTN--NNINEIETVKVCLGDNDHAEAHQAMVTTYE 958
             KHDDANNN SH GIGL SE+KQEH N  +N+NE ET KVCLGDNDHAEA QAMVTTYE
Sbjct: 901 --KHDDANNNTSHSGIGLFSETKQEHANKNDNVNENETGKVCLGDNDHAEADQAMVTTYE 934

BLAST of Cucsat.G13224 vs. NCBI nr
Match: XP_008443321.1 (PREDICTED: putative disease resistance protein RGA3 isoform X3 [Cucumis melo])

HSP 1 Score: 1417 bits (3669), Expect = 0.0
Identity = 753/982 (76.68%), Postives = 820/982 (83.50%), Query Frame = 0

Query: 1   MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN 60
           MVGLL SVAGNLLGRIIEAADR EFRAI+ ELKNLET VL+LKARLRDAEEKQAS+ EL 
Sbjct: 1   MVGLLGSVAGNLLGRIIEAADRREFRAIRRELKNLETAVLDLKARLRDAEEKQASDPELY 60

Query: 61  ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS 120
           + L+ LK+ FS AD  IEELECDYLKWRVQNRKNDVDDKG QFSSCFSSNF IS  NT +
Sbjct: 61  DQLRKLKHAFSMADSVIEELECDYLKWRVQNRKNDVDDKGYQFSSCFSSNFPISSCNTVA 120

Query: 121 KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR 180
           KFQ+    +T ELR IE+ MSKFSLVEDEDEYIK LKGEMTLRTSITGS AFARLLRLR+
Sbjct: 121 KFQQ----LTEELRLIEETMSKFSLVEDEDEYIKNLKGEMTLRTSITGSQAFARLLRLRK 180

Query: 181 EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240
           EAILSNVDSIFGRDK+QESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD
Sbjct: 181 EAILSNVDSIFGRDKVQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240

Query: 241 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300
           HFDKR+WVCVSEDFDI+RI++EVL+SATGENVTTVALTESRLRIRLQRYFFGKKILLVLD
Sbjct: 241 HFDKRIWVCVSEDFDIQRIIKEVLISATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300

Query: 301 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY 360
           DFGNLD +RVSELK+IVKMGV GSKIMITTRS +T NVA THKIDKLD+TISMQIFEDT+
Sbjct: 301 DFGNLDHDRVSELKEIVKMGVDGSKIMITTRSHKTPNVAATHKIDKLDKTISMQIFEDTF 360

Query: 361 GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE 420
           G   LS    +D+ LKNLVAECGGAPLAIKCLAGLLSSKPSDGA SPNVKDLSEKWK EE
Sbjct: 361 GGNELS----NDVDLKNLVAECGGAPLAIKCLAGLLSSKPSDGANSPNVKDLSEKWKLEE 420

Query: 421 ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN 480
           AN GGGV CALRLS                                          TKD+
Sbjct: 421 ANYGGGVSCALRLS------------------------------------------TKDD 480

Query: 481 PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD 540
           PEE+GEKYFKE  DRRLLVDVEEHTLGYWFKIH+LVHDLAVQKA  QKNLG FH LSFVD
Sbjct: 481 PEEIGEKYFKELWDRRLLVDVEEHTLGYWFKIHNLVHDLAVQKAEGQKNLGIFHQLSFVD 540

Query: 541 CDS-IPSSTNYDNTRFISIPVVGGAGPNINSD-LFKCITQFRQLRFLYLCNSSLEEIPTS 600
           C S IP ST+Y++  F+SIPVVGGA P IN D LFKCIT+F+QLRFLYLCNSSLEEIPTS
Sbjct: 541 CKSNIPPSTSYNDIHFLSIPVVGGAEPKINGDPLFKCITKFKQLRFLYLCNSSLEEIPTS 600

Query: 601 IDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWI 660
           I TLKHLRCLDLRGSQRLKRLPESICKLQSL+TL+LAFCSELEELPRNIKNLISLRFLW+
Sbjct: 601 IGTLKHLRCLDLRGSQRLKRLPESICKLQSLRTLILAFCSELEELPRNIKNLISLRFLWV 660

Query: 661 QTKQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGL 720
           QTKQA L KD+IGSLTSLRFLAIGRS+NLTHLFEDI+KLN LKTLIIY+CKSLLTLPKGL
Sbjct: 661 QTKQASLGKDQIGSLTSLRFLAIGRSDNLTHLFEDIDKLNFLKTLIIYDCKSLLTLPKGL 720

Query: 721 ENMKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGE 780
           EN++S+CNMGIW C+RLRFTFSLASL+LKKLILR+LTAVS+LP WL++LD TLEVLEIGE
Sbjct: 721 ENVESLCNMGIWGCERLRFTFSLASLNLKKLILRQLTAVSSLPKWLAHLDDTLEVLEIGE 780

Query: 781 FPTLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLL 840
           FPTLR+LP+W  N+WELRILGISNCP+L H+ F  EL  + DKIEELR+TFCGS SKS  
Sbjct: 781 FPTLRELPMWFSNYWELRILGISNCPQLNHECFHHELPIYRDKIEELRVTFCGSSSKSSW 840

Query: 841 KKSMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNN---------- 900
           KKSM+EI+ ++  I YI  IYVDSKR+ P  EST++PKEAETKQDDA NN          
Sbjct: 841 KKSMEEIKNKNPNISYIRAIYVDSKRIMPLEESTEKPKEAETKQDDANNNMSHVDIGLLS 900

Query: 901 ----------ASPPGTEQPSKTKHDDANNNMSHPGIGLLSESKQEHTN--NNINEIETVK 958
                      S PGT+QPS  KHDDANNN SH GIGL SE+KQEH N  +N+NE ET K
Sbjct: 901 KIEQAHANNNVSHPGTKQPSMNKHDDANNNTSHSGIGLFSETKQEHANKNDNVNENETGK 932

BLAST of Cucsat.G13224 vs. NCBI nr
Match: XP_008443322.1 (PREDICTED: putative disease resistance protein RGA1 isoform X4 [Cucumis melo])

HSP 1 Score: 1250 bits (3234), Expect = 0.0
Identity = 685/981 (69.83%), Postives = 746/981 (76.04%), Query Frame = 0

Query: 1   MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN 60
           MVGLL SVAGNLLGRIIEAADR EFRAI+ ELKNLET VL+LKARLRDAEEKQAS+ EL 
Sbjct: 1   MVGLLGSVAGNLLGRIIEAADRREFRAIRRELKNLETAVLDLKARLRDAEEKQASDPELY 60

Query: 61  ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS 120
           + L+ LK+ FS AD  IEELECDYLKWRVQNRKNDVDDKG QFSSCFSSNF IS  NT +
Sbjct: 61  DQLRKLKHAFSMADSVIEELECDYLKWRVQNRKNDVDDKGYQFSSCFSSNFPISSCNTVA 120

Query: 121 KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR 180
           KFQ+    +T ELR IE+ MSKFSLVEDEDEYIK LKGEMTLRTSITGS AFARLLRLR+
Sbjct: 121 KFQQ----LTEELRLIEETMSKFSLVEDEDEYIKNLKGEMTLRTSITGSQAFARLLRLRK 180

Query: 181 EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240
           EAILSNVDSIFGRDK+QESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD
Sbjct: 181 EAILSNVDSIFGRDKVQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240

Query: 241 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300
           HFDKR+WVCVSEDFDI+RI++EVL+SATGENVTTVALTESRLRIRLQRYFFGKKILLVLD
Sbjct: 241 HFDKRIWVCVSEDFDIQRIIKEVLISATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300

Query: 301 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY 360
           DFGNLD +RVSELK+IVKMGV GSKIMITTRS +T NVA THKIDKLD+TISMQIFEDT+
Sbjct: 301 DFGNLDHDRVSELKEIVKMGVDGSKIMITTRSHKTPNVAATHKIDKLDKTISMQIFEDTF 360

Query: 361 GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE 420
           G   LS    +D+ LKNLVAECGGAPLAIKCLAGLLSSKPSDGA SPNVKDLSEKWK EE
Sbjct: 361 GGNELS----NDVDLKNLVAECGGAPLAIKCLAGLLSSKPSDGANSPNVKDLSEKWKLEE 420

Query: 421 ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN 480
           AN GGGV CALRL                                               
Sbjct: 421 ANYGGGVSCALRL----------------------------------------------- 480

Query: 481 PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD 540
                                                                       
Sbjct: 481 ------------------------------------------------------------ 540

Query: 541 CDSIPSSTNYDNTRFISIPVVGGAGPNINSD-LFKCITQFRQLRFLYLCNSSLEEIPTSI 600
                              +VGGA P IN D LFKCIT+F+QLRFLYLCNSSLEEIPTSI
Sbjct: 541 -------------------IVGGAEPKINGDPLFKCITKFKQLRFLYLCNSSLEEIPTSI 600

Query: 601 DTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWIQ 660
            TLKHLRCLDLRGSQRLKRLPESICKLQSL+TL+LAFCSELEELPRNIKNLISLRFLW+Q
Sbjct: 601 GTLKHLRCLDLRGSQRLKRLPESICKLQSLRTLILAFCSELEELPRNIKNLISLRFLWVQ 660

Query: 661 TKQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGLE 720
           TKQA L KD+IGSLTSLRFLAIGRS+NLTHLFEDI+KLN LKTLIIY+CKSLLTLPKGLE
Sbjct: 661 TKQASLGKDQIGSLTSLRFLAIGRSDNLTHLFEDIDKLNFLKTLIIYDCKSLLTLPKGLE 720

Query: 721 NMKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGEF 780
           N++S+CNMGIW C+RLRFTFSLASL+LKKLILR+LTAVS+LP WL++LD TLEVLEIGEF
Sbjct: 721 NVESLCNMGIWGCERLRFTFSLASLNLKKLILRQLTAVSSLPKWLAHLDDTLEVLEIGEF 780

Query: 781 PTLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLLK 840
           PTLR+LP+W  N+WELRILGISNCP+L H+ F  EL  + DKIEELR+TFCGS SKS  K
Sbjct: 781 PTLRELPMWFSNYWELRILGISNCPQLNHECFHHELPIYRDKIEELRVTFCGSSSKSSWK 840

Query: 841 KSMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNN----------- 900
           KSM+EI+ ++  I YI  IYVDSKR+ P  EST++PKEAETKQDDA NN           
Sbjct: 841 KSMEEIKNKNPNISYIRAIYVDSKRIMPLEESTEKPKEAETKQDDANNNMSHVDIGLLSK 847

Query: 901 ---------ASPPGTEQPSKTKHDDANNNMSHPGIGLLSESKQEHTN--NNINEIETVKV 958
                     S PGT+QPS  KHDDANNN SH GIGL SE+KQEH N  +N+NE ET KV
Sbjct: 901 IEQAHANNNVSHPGTKQPSMNKHDDANNNTSHSGIGLFSETKQEHANKNDNVNENETGKV 847

BLAST of Cucsat.G13224 vs. ExPASy TrEMBL
Match: A0A5A7UJY5 (Putative disease resistance protein RGA3 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007240 PE=4 SV=1)

HSP 1 Score: 1515 bits (3922), Expect = 0.0
Identity = 789/982 (80.35%), Postives = 859/982 (87.47%), Query Frame = 0

Query: 1   MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN 60
           MVGLL SVAGNLLGRIIEAADR EFRAI+ ELKNLET VL+LKARLRDAEEKQAS+ EL 
Sbjct: 1   MVGLLGSVAGNLLGRIIEAADRREFRAIRRELKNLETAVLDLKARLRDAEEKQASDPELY 60

Query: 61  ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS 120
           + L+ LK+ FS AD  IEELECDYLKWRVQNRKNDVDDKG QFSSCFSSNF IS  NT +
Sbjct: 61  DQLRKLKHAFSMADSVIEELECDYLKWRVQNRKNDVDDKGYQFSSCFSSNFPISSCNTVA 120

Query: 121 KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR 180
           KFQ+    +T ELR IE+ MSKFSLVEDEDEYIK LKGEMTLRTSITGS AFARLLRLR+
Sbjct: 121 KFQQ----LTEELRLIEETMSKFSLVEDEDEYIKNLKGEMTLRTSITGSQAFARLLRLRK 180

Query: 181 EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240
           EAILSNVDSIFGRDK+QESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD
Sbjct: 181 EAILSNVDSIFGRDKVQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240

Query: 241 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300
           HFDKR+WVCVSEDFDI+RI++EVL+SATGENVTTVALTESRLRIRLQRYFFGKKILLVLD
Sbjct: 241 HFDKRIWVCVSEDFDIQRIIKEVLISATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300

Query: 301 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY 360
           DFGNLD +RVSELK+IVKMGV GSKIMITTRS +T NVA THKIDKLD+TISMQIFEDT+
Sbjct: 301 DFGNLDHDRVSELKEIVKMGVDGSKIMITTRSHKTPNVAATHKIDKLDKTISMQIFEDTF 360

Query: 361 GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE 420
           G   LS    +D+ LKNLVAECGGAPLAIKCLAGLLSSKPSDGA SPNVKDLSEKWK EE
Sbjct: 361 GGNELS----NDVDLKNLVAECGGAPLAIKCLAGLLSSKPSDGANSPNVKDLSEKWKLEE 420

Query: 421 ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN 480
           AN GGGV CALRLSYDLMPSYLKP FLCFS+LP+DNVFFSFELIQLW+ QG LPSGTKD+
Sbjct: 421 ANYGGGVSCALRLSYDLMPSYLKPFFLCFSLLPRDNVFFSFELIQLWLEQGFLPSGTKDD 480

Query: 481 PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD 540
           PEE+GEKYFKE  DRRLLVDVEEHTLGYWFKIH+LVHDLAVQKA  QKNLG FH LSFVD
Sbjct: 481 PEEIGEKYFKELWDRRLLVDVEEHTLGYWFKIHNLVHDLAVQKAEGQKNLGIFHQLSFVD 540

Query: 541 CDS-IPSSTNYDNTRFISIPVVGGAGPNINSD-LFKCITQFRQLRFLYLCNSSLEEIPTS 600
           C S IP ST+Y++  F+SIPVVGGA P IN D LFKCIT+F+QLRFLYLCNSSLEEIPTS
Sbjct: 541 CKSNIPPSTSYNDIHFLSIPVVGGAEPKINGDPLFKCITKFKQLRFLYLCNSSLEEIPTS 600

Query: 601 IDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWI 660
           I TLKHLRCLDLRGSQRLKRLPESICKLQSL+TL+LAFCSELEELPRNIKNLISLRFLW+
Sbjct: 601 IGTLKHLRCLDLRGSQRLKRLPESICKLQSLRTLILAFCSELEELPRNIKNLISLRFLWV 660

Query: 661 QTKQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGL 720
           QTKQA L KD+IGSLTSLRFLAIGRS+NLTHLFEDI+KLN LKTLIIY+CKSLLTLPKGL
Sbjct: 661 QTKQASLGKDQIGSLTSLRFLAIGRSDNLTHLFEDIDKLNFLKTLIIYDCKSLLTLPKGL 720

Query: 721 ENMKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGE 780
           EN++S+CNMGIW C+RLRFTFSLASL+LKKLILR+LTAVS+LP WL++LD TLEVLEIGE
Sbjct: 721 ENVESLCNMGIWGCERLRFTFSLASLNLKKLILRQLTAVSSLPKWLAHLDDTLEVLEIGE 780

Query: 781 FPTLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLL 840
           FPTLR+LP+W  N+WELRILGISNCP+L H+ F  EL  + DKIEELR+TFCGS SKS  
Sbjct: 781 FPTLRELPMWFSNYWELRILGISNCPQLNHECFHHELPIYRDKIEELRVTFCGSSSKSSW 840

Query: 841 KKSMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNN---------- 900
           KKSM+EI+ ++  I YI  IYVDSKR+ P  EST++PKEAETKQDDA NN          
Sbjct: 841 KKSMEEIKNKNPNISYIRAIYVDSKRIMPLEESTEKPKEAETKQDDANNNMSHVDIGLLS 900

Query: 901 ----------ASPPGTEQPSKTKHDDANNNMSHPGIGLLSESKQEHTN--NNINEIETVK 958
                      S PGT+QPS  KHDDANNN SH GIGL SE+KQEH N  +N+NE ET K
Sbjct: 901 KIEQAHANNNVSHPGTKQPSMNKHDDANNNTSHSGIGLFSETKQEHANKNDNVNENETGK 960

BLAST of Cucsat.G13224 vs. ExPASy TrEMBL
Match: A0A1S3B8G3 (putative disease resistance protein RGA4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486933 PE=4 SV=1)

HSP 1 Score: 1491 bits (3861), Expect = 0.0
Identity = 775/962 (80.56%), Postives = 844/962 (87.73%), Query Frame = 0

Query: 1   MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN 60
           MVGLL SVAGNLLGRIIEAADR EFRAI+ ELKNLET VL+LKARLRDAEEKQAS+ EL 
Sbjct: 1   MVGLLGSVAGNLLGRIIEAADRREFRAIRRELKNLETAVLDLKARLRDAEEKQASDPELY 60

Query: 61  ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS 120
           + L+ LK+ FS AD  IEELECDYLKWRVQNRKNDVDDKG QFSSCFSSNF IS  NT +
Sbjct: 61  DQLRKLKHAFSMADSVIEELECDYLKWRVQNRKNDVDDKGYQFSSCFSSNFPISSCNTVA 120

Query: 121 KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR 180
           KFQ+    +T ELR IE+ MSKFSLVEDEDEYIK LKGEMTLRTSITGS AFARLLRLR+
Sbjct: 121 KFQQ----LTEELRLIEETMSKFSLVEDEDEYIKNLKGEMTLRTSITGSQAFARLLRLRK 180

Query: 181 EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240
           EAILSNVDSIFGRDK+QESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD
Sbjct: 181 EAILSNVDSIFGRDKVQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240

Query: 241 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300
           HFDKR+WVCVSEDFDI+RI++EVL+SATGENVTTVALTESRLRIRLQRYFFGKKILLVLD
Sbjct: 241 HFDKRIWVCVSEDFDIQRIIKEVLISATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300

Query: 301 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY 360
           DFGNLD +RVSELK+IVKMGV GSKIMITTRS +T NVA THKIDKLD+TISMQIFEDT+
Sbjct: 301 DFGNLDHDRVSELKEIVKMGVDGSKIMITTRSHKTPNVAATHKIDKLDKTISMQIFEDTF 360

Query: 361 GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE 420
           G   LS    +D+ LKNLVAECGGAPLAIKCLAGLLSSKPSDGA SPNVKDLSEKWK EE
Sbjct: 361 GGNELS----NDVDLKNLVAECGGAPLAIKCLAGLLSSKPSDGANSPNVKDLSEKWKLEE 420

Query: 421 ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN 480
           AN GGGV CALRLSYDLMPSYLKP FLCFS+LP+DNVFFSFELIQLW+ QG LPSGTKD+
Sbjct: 421 ANYGGGVSCALRLSYDLMPSYLKPFFLCFSLLPRDNVFFSFELIQLWLEQGFLPSGTKDD 480

Query: 481 PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD 540
           PEE+GEKYFKE  DRRLLVDVEEHTLGYWFKIH+LVHDLAVQKA  QKNLG FH LSFVD
Sbjct: 481 PEEIGEKYFKELWDRRLLVDVEEHTLGYWFKIHNLVHDLAVQKAEGQKNLGIFHQLSFVD 540

Query: 541 CDS-IPSSTNYDNTRFISIPVVGGAGPNINSD-LFKCITQFRQLRFLYLCNSSLEEIPTS 600
           C S IP ST+Y++  F+SIPVVGGA P IN D LFKCIT+F+QLRFLYLCNSSLEEIPTS
Sbjct: 541 CKSNIPPSTSYNDIHFLSIPVVGGAEPKINGDPLFKCITKFKQLRFLYLCNSSLEEIPTS 600

Query: 601 IDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWI 660
           I TLKHLRCLDLRGSQRLKRLPESICKLQSL+TL+LAFCSELEELPRNIKNLISLRFLW+
Sbjct: 601 IGTLKHLRCLDLRGSQRLKRLPESICKLQSLRTLILAFCSELEELPRNIKNLISLRFLWV 660

Query: 661 QTKQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGL 720
           QTKQA L KD+IGSLTSLRFLAIGRS+NLTHLFEDI+KLN LKTLIIY+CKSLLTLPKGL
Sbjct: 661 QTKQASLGKDQIGSLTSLRFLAIGRSDNLTHLFEDIDKLNFLKTLIIYDCKSLLTLPKGL 720

Query: 721 ENMKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGE 780
           EN++S+CNMGIW C+RLRFTFSLASL+LKKLILR+LTAVS+LP WL++LD TLEVLEIGE
Sbjct: 721 ENVESLCNMGIWGCERLRFTFSLASLNLKKLILRQLTAVSSLPKWLAHLDDTLEVLEIGE 780

Query: 781 FPTLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLL 840
           FPTLR+LP+W  N+WELRILGISNCP+L H+ F  EL  + DKIEELR+TFCGS SKS  
Sbjct: 781 FPTLRELPMWFSNYWELRILGISNCPQLNHECFHHELPIYRDKIEELRVTFCGSSSKSSW 840

Query: 841 KKSMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNNASPPGTEQPS 900
           KKSM+EI+ ++  I YI  IYVDSKR+ P  EST++PKEAET                  
Sbjct: 841 KKSMEEIKNKNPNISYIRAIYVDSKRIMPLEESTEKPKEAET------------------ 900

Query: 901 KTKHDDANNNMSHPGIGLLSESKQEHTN--NNINEIETVKVCLGDNDHAEAHQAMVTTYE 958
             KHDDANNN SH GIGL SE+KQEH N  +N+NE ET KVCLGDNDHAEA QAMVTTYE
Sbjct: 901 --KHDDANNNTSHSGIGLFSETKQEHANKNDNVNENETGKVCLGDNDHAEADQAMVTTYE 934

BLAST of Cucsat.G13224 vs. ExPASy TrEMBL
Match: A0A1S3B8I2 (putative disease resistance protein RGA3 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103486933 PE=4 SV=1)

HSP 1 Score: 1417 bits (3669), Expect = 0.0
Identity = 753/982 (76.68%), Postives = 820/982 (83.50%), Query Frame = 0

Query: 1   MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN 60
           MVGLL SVAGNLLGRIIEAADR EFRAI+ ELKNLET VL+LKARLRDAEEKQAS+ EL 
Sbjct: 1   MVGLLGSVAGNLLGRIIEAADRREFRAIRRELKNLETAVLDLKARLRDAEEKQASDPELY 60

Query: 61  ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS 120
           + L+ LK+ FS AD  IEELECDYLKWRVQNRKNDVDDKG QFSSCFSSNF IS  NT +
Sbjct: 61  DQLRKLKHAFSMADSVIEELECDYLKWRVQNRKNDVDDKGYQFSSCFSSNFPISSCNTVA 120

Query: 121 KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR 180
           KFQ+    +T ELR IE+ MSKFSLVEDEDEYIK LKGEMTLRTSITGS AFARLLRLR+
Sbjct: 121 KFQQ----LTEELRLIEETMSKFSLVEDEDEYIKNLKGEMTLRTSITGSQAFARLLRLRK 180

Query: 181 EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240
           EAILSNVDSIFGRDK+QESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD
Sbjct: 181 EAILSNVDSIFGRDKVQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240

Query: 241 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300
           HFDKR+WVCVSEDFDI+RI++EVL+SATGENVTTVALTESRLRIRLQRYFFGKKILLVLD
Sbjct: 241 HFDKRIWVCVSEDFDIQRIIKEVLISATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300

Query: 301 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY 360
           DFGNLD +RVSELK+IVKMGV GSKIMITTRS +T NVA THKIDKLD+TISMQIFEDT+
Sbjct: 301 DFGNLDHDRVSELKEIVKMGVDGSKIMITTRSHKTPNVAATHKIDKLDKTISMQIFEDTF 360

Query: 361 GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE 420
           G   LS    +D+ LKNLVAECGGAPLAIKCLAGLLSSKPSDGA SPNVKDLSEKWK EE
Sbjct: 361 GGNELS----NDVDLKNLVAECGGAPLAIKCLAGLLSSKPSDGANSPNVKDLSEKWKLEE 420

Query: 421 ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN 480
           AN GGGV CALRLS                                          TKD+
Sbjct: 421 ANYGGGVSCALRLS------------------------------------------TKDD 480

Query: 481 PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD 540
           PEE+GEKYFKE  DRRLLVDVEEHTLGYWFKIH+LVHDLAVQKA  QKNLG FH LSFVD
Sbjct: 481 PEEIGEKYFKELWDRRLLVDVEEHTLGYWFKIHNLVHDLAVQKAEGQKNLGIFHQLSFVD 540

Query: 541 CDS-IPSSTNYDNTRFISIPVVGGAGPNINSD-LFKCITQFRQLRFLYLCNSSLEEIPTS 600
           C S IP ST+Y++  F+SIPVVGGA P IN D LFKCIT+F+QLRFLYLCNSSLEEIPTS
Sbjct: 541 CKSNIPPSTSYNDIHFLSIPVVGGAEPKINGDPLFKCITKFKQLRFLYLCNSSLEEIPTS 600

Query: 601 IDTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWI 660
           I TLKHLRCLDLRGSQRLKRLPESICKLQSL+TL+LAFCSELEELPRNIKNLISLRFLW+
Sbjct: 601 IGTLKHLRCLDLRGSQRLKRLPESICKLQSLRTLILAFCSELEELPRNIKNLISLRFLWV 660

Query: 661 QTKQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGL 720
           QTKQA L KD+IGSLTSLRFLAIGRS+NLTHLFEDI+KLN LKTLIIY+CKSLLTLPKGL
Sbjct: 661 QTKQASLGKDQIGSLTSLRFLAIGRSDNLTHLFEDIDKLNFLKTLIIYDCKSLLTLPKGL 720

Query: 721 ENMKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGE 780
           EN++S+CNMGIW C+RLRFTFSLASL+LKKLILR+LTAVS+LP WL++LD TLEVLEIGE
Sbjct: 721 ENVESLCNMGIWGCERLRFTFSLASLNLKKLILRQLTAVSSLPKWLAHLDDTLEVLEIGE 780

Query: 781 FPTLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLL 840
           FPTLR+LP+W  N+WELRILGISNCP+L H+ F  EL  + DKIEELR+TFCGS SKS  
Sbjct: 781 FPTLRELPMWFSNYWELRILGISNCPQLNHECFHHELPIYRDKIEELRVTFCGSSSKSSW 840

Query: 841 KKSMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNN---------- 900
           KKSM+EI+ ++  I YI  IYVDSKR+ P  EST++PKEAETKQDDA NN          
Sbjct: 841 KKSMEEIKNKNPNISYIRAIYVDSKRIMPLEESTEKPKEAETKQDDANNNMSHVDIGLLS 900

Query: 901 ----------ASPPGTEQPSKTKHDDANNNMSHPGIGLLSESKQEHTN--NNINEIETVK 958
                      S PGT+QPS  KHDDANNN SH GIGL SE+KQEH N  +N+NE ET K
Sbjct: 901 KIEQAHANNNVSHPGTKQPSMNKHDDANNNTSHSGIGLFSETKQEHANKNDNVNENETGK 932

BLAST of Cucsat.G13224 vs. ExPASy TrEMBL
Match: A0A0A0LBX7 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G822460 PE=4 SV=1)

HSP 1 Score: 1390 bits (3598), Expect = 0.0
Identity = 694/694 (100.00%), Postives = 694/694 (100.00%), Query Frame = 0

Query: 265 MSATGENVTTVALTESRLRIRLQRYFFGKKILLVLDDFGNLDPERVSELKKIVKMGVGGS 324
           MSATGENVTTVALTESRLRIRLQRYFFGKKILLVLDDFGNLDPERVSELKKIVKMGVGGS
Sbjct: 1   MSATGENVTTVALTESRLRIRLQRYFFGKKILLVLDDFGNLDPERVSELKKIVKMGVGGS 60

Query: 325 KIMITTRSDETLNVATTHKIDKLDETISMQIFEDTYGSEGLSEGLRDDLYLKNLVAECGG 384
           KIMITTRSDETLNVATTHKIDKLDETISMQIFEDTYGSEGLSEGLRDDLYLKNLVAECGG
Sbjct: 61  KIMITTRSDETLNVATTHKIDKLDETISMQIFEDTYGSEGLSEGLRDDLYLKNLVAECGG 120

Query: 385 APLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEEANNGGGVLCALRLSYDLMPSYLKP 444
           APLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEEANNGGGVLCALRLSYDLMPSYLKP
Sbjct: 121 APLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEEANNGGGVLCALRLSYDLMPSYLKP 180

Query: 445 CFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDNPEEVGEKYFKEFRDRRLLVDVEEH 504
           CFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDNPEEVGEKYFKEFRDRRLLVDVEEH
Sbjct: 181 CFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDNPEEVGEKYFKEFRDRRLLVDVEEH 240

Query: 505 TLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVDCDSIPSSTNYDNTRFISIPVVGGA 564
           TLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVDCDSIPSSTNYDNTRFISIPVVGGA
Sbjct: 241 TLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVDCDSIPSSTNYDNTRFISIPVVGGA 300

Query: 565 GPNINSDLFKCITQFRQLRFLYLCNSSLEEIPTSIDTLKHLRCLDLRGSQRLKRLPESIC 624
           GPNINSDLFKCITQFRQLRFLYLCNSSLEEIPTSIDTLKHLRCLDLRGSQRLKRLPESIC
Sbjct: 301 GPNINSDLFKCITQFRQLRFLYLCNSSLEEIPTSIDTLKHLRCLDLRGSQRLKRLPESIC 360

Query: 625 KLQSLQTLVLAFCSELEELPRNIKNLISLRFLWIQTKQARLEKDEIGSLTSLRFLAIGRS 684
           KLQSLQTLVLAFCSELEELPRNIKNLISLRFLWIQTKQARLEKDEIGSLTSLRFLAIGRS
Sbjct: 361 KLQSLQTLVLAFCSELEELPRNIKNLISLRFLWIQTKQARLEKDEIGSLTSLRFLAIGRS 420

Query: 685 ENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGLENMKSICNMGIWECDRLRFTFSLASL 744
           ENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGLENMKSICNMGIWECDRLRFTFSLASL
Sbjct: 421 ENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGLENMKSICNMGIWECDRLRFTFSLASL 480

Query: 745 HLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGEFPTLRKLPIWLLNFWELRILGISNCP 804
           HLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGEFPTLRKLPIWLLNFWELRILGISNCP
Sbjct: 481 HLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGEFPTLRKLPIWLLNFWELRILGISNCP 540

Query: 805 KLKHDSFPPELNYFCDKIEELRITFCGSLSKSLLKKSMKEIEPESRVIFYIHTIYVDSKR 864
           KLKHDSFPPELNYFCDKIEELRITFCGSLSKSLLKKSMKEIEPESRVIFYIHTIYVDSKR
Sbjct: 541 KLKHDSFPPELNYFCDKIEELRITFCGSLSKSLLKKSMKEIEPESRVIFYIHTIYVDSKR 600

Query: 865 MTPPVESTDEPKEAETKQDDAYNNASPPGTEQPSKTKHDDANNNMSHPGIGLLSESKQEH 924
           MTPPVESTDEPKEAETKQDDAYNNASPPGTEQPSKTKHDDANNNMSHPGIGLLSESKQEH
Sbjct: 601 MTPPVESTDEPKEAETKQDDAYNNASPPGTEQPSKTKHDDANNNMSHPGIGLLSESKQEH 660

Query: 925 TNNNINEIETVKVCLGDNDHAEAHQAMVTTYEGF 958
           TNNNINEIETVKVCLGDNDHAEAHQAMVTTYEGF
Sbjct: 661 TNNNINEIETVKVCLGDNDHAEAHQAMVTTYEGF 694

BLAST of Cucsat.G13224 vs. ExPASy TrEMBL
Match: A0A1S3B798 (putative disease resistance protein RGA1 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103486933 PE=4 SV=1)

HSP 1 Score: 1250 bits (3234), Expect = 0.0
Identity = 685/981 (69.83%), Postives = 746/981 (76.04%), Query Frame = 0

Query: 1   MVGLLDSVAGNLLGRIIEAADRLEFRAIQSELKNLETDVLNLKARLRDAEEKQASNCELN 60
           MVGLL SVAGNLLGRIIEAADR EFRAI+ ELKNLET VL+LKARLRDAEEKQAS+ EL 
Sbjct: 1   MVGLLGSVAGNLLGRIIEAADRREFRAIRRELKNLETAVLDLKARLRDAEEKQASDPELY 60

Query: 61  ELLKNLKNVFSRADIAIEELECDYLKWRVQNRKNDVDDKGCQFSSCFSSNFLISPFNTGS 120
           + L+ LK+ FS AD  IEELECDYLKWRVQNRKNDVDDKG QFSSCFSSNF IS  NT +
Sbjct: 61  DQLRKLKHAFSMADSVIEELECDYLKWRVQNRKNDVDDKGYQFSSCFSSNFPISSCNTVA 120

Query: 121 KFQEDLKIITSELRSIEKAMSKFSLVEDEDEYIKKLKGEMTLRTSITGSHAFARLLRLRR 180
           KFQ+    +T ELR IE+ MSKFSLVEDEDEYIK LKGEMTLRTSITGS AFARLLRLR+
Sbjct: 121 KFQQ----LTEELRLIEETMSKFSLVEDEDEYIKNLKGEMTLRTSITGSQAFARLLRLRK 180

Query: 181 EAILSNVDSIFGRDKIQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240
           EAILSNVDSIFGRDK+QESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD
Sbjct: 181 EAILSNVDSIFGRDKVQESIIKELVNDEQKSPRILSIQGDGGMGKTALAKLVYNADEVFD 240

Query: 241 HFDKRMWVCVSEDFDIRRILREVLMSATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300
           HFDKR+WVCVSEDFDI+RI++EVL+SATGENVTTVALTESRLRIRLQRYFFGKKILLVLD
Sbjct: 241 HFDKRIWVCVSEDFDIQRIIKEVLISATGENVTTVALTESRLRIRLQRYFFGKKILLVLD 300

Query: 301 DFGNLDPERVSELKKIVKMGVGGSKIMITTRSDETLNVATTHKIDKLDETISMQIFEDTY 360
           DFGNLD +RVSELK+IVKMGV GSKIMITTRS +T NVA THKIDKLD+TISMQIFEDT+
Sbjct: 301 DFGNLDHDRVSELKEIVKMGVDGSKIMITTRSHKTPNVAATHKIDKLDKTISMQIFEDTF 360

Query: 361 GSEGLSEGLRDDLYLKNLVAECGGAPLAIKCLAGLLSSKPSDGAKSPNVKDLSEKWKQEE 420
           G   LS    +D+ LKNLVAECGGAPLAIKCLAGLLSSKPSDGA SPNVKDLSEKWK EE
Sbjct: 361 GGNELS----NDVDLKNLVAECGGAPLAIKCLAGLLSSKPSDGANSPNVKDLSEKWKLEE 420

Query: 421 ANNGGGVLCALRLSYDLMPSYLKPCFLCFSVLPKDNVFFSFELIQLWMAQGILPSGTKDN 480
           AN GGGV CALRL                                               
Sbjct: 421 ANYGGGVSCALRL----------------------------------------------- 480

Query: 481 PEEVGEKYFKEFRDRRLLVDVEEHTLGYWFKIHSLVHDLAVQKATEQKNLGNFHMLSFVD 540
                                                                       
Sbjct: 481 ------------------------------------------------------------ 540

Query: 541 CDSIPSSTNYDNTRFISIPVVGGAGPNINSD-LFKCITQFRQLRFLYLCNSSLEEIPTSI 600
                              +VGGA P IN D LFKCIT+F+QLRFLYLCNSSLEEIPTSI
Sbjct: 541 -------------------IVGGAEPKINGDPLFKCITKFKQLRFLYLCNSSLEEIPTSI 600

Query: 601 DTLKHLRCLDLRGSQRLKRLPESICKLQSLQTLVLAFCSELEELPRNIKNLISLRFLWIQ 660
            TLKHLRCLDLRGSQRLKRLPESICKLQSL+TL+LAFCSELEELPRNIKNLISLRFLW+Q
Sbjct: 601 GTLKHLRCLDLRGSQRLKRLPESICKLQSLRTLILAFCSELEELPRNIKNLISLRFLWVQ 660

Query: 661 TKQARLEKDEIGSLTSLRFLAIGRSENLTHLFEDINKLNSLKTLIIYECKSLLTLPKGLE 720
           TKQA L KD+IGSLTSLRFLAIGRS+NLTHLFEDI+KLN LKTLIIY+CKSLLTLPKGLE
Sbjct: 661 TKQASLGKDQIGSLTSLRFLAIGRSDNLTHLFEDIDKLNFLKTLIIYDCKSLLTLPKGLE 720

Query: 721 NMKSICNMGIWECDRLRFTFSLASLHLKKLILRELTAVSTLPNWLSNLDGTLEVLEIGEF 780
           N++S+CNMGIW C+RLRFTFSLASL+LKKLILR+LTAVS+LP WL++LD TLEVLEIGEF
Sbjct: 721 NVESLCNMGIWGCERLRFTFSLASLNLKKLILRQLTAVSSLPKWLAHLDDTLEVLEIGEF 780

Query: 781 PTLRKLPIWLLNFWELRILGISNCPKLKHDSFPPELNYFCDKIEELRITFCGSLSKSLLK 840
           PTLR+LP+W  N+WELRILGISNCP+L H+ F  EL  + DKIEELR+TFCGS SKS  K
Sbjct: 781 PTLRELPMWFSNYWELRILGISNCPQLNHECFHHELPIYRDKIEELRVTFCGSSSKSSWK 840

Query: 841 KSMKEIEPESRVIFYIHTIYVDSKRMTPPVESTDEPKEAETKQDDAYNN----------- 900
           KSM+EI+ ++  I YI  IYVDSKR+ P  EST++PKEAETKQDDA NN           
Sbjct: 841 KSMEEIKNKNPNISYIRAIYVDSKRIMPLEESTEKPKEAETKQDDANNNMSHVDIGLLSK 847

Query: 901 ---------ASPPGTEQPSKTKHDDANNNMSHPGIGLLSESKQEHTN--NNINEIETVKV 958
                     S PGT+QPS  KHDDANNN SH GIGL SE+KQEH N  +N+NE ET KV
Sbjct: 901 IEQAHANNNVSHPGTKQPSMNKHDDANNNTSHSGIGLFSETKQEHANKNDNVNENETGKV 847