Cucsat.G12697 (gene) Cucumber (B10) v3

BLAST of Cucsat.G12697 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.5e-112
Identity = 317/979 (32.38%), Postives = 496/979 (50.66%), Query Frame = 0

Query: 9   VQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGNSVKRWVEK 68
           +Q +L  +  +   ++V+ +  ++E   L         +LED   K+ +    ++ W++K
Sbjct: 6   IQVLLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLN-NKPLENWLQK 65

Query: 69  LEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFL--FRRKMAKKIKNIT 128
           L    +E DD+LD           E+  K ++ S S     +  +  FR K+ K++  + 
Sbjct: 66  LNAATYEVDDILD-----------EYKTKATRFSQSEYGRYHPKVIPFRHKVGKRMDQVM 125

Query: 129 DTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSIL-DFQVEGREAEVLELLKLAIDS 188
             L         F L   E + E +      RET S+L + QV GR+ E  E++K+ I++
Sbjct: 126 KKLKAIAEERKNFHL--HEKIVERQAVR---RETGSVLTEPQVYGRDKEKDEIVKILINN 185

Query: 189 TNE-HHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILEKIFQ 248
            ++  H+SV+ I+GMGGLGKTTLA+M+FN + +  HF   IW+CVS+ F   ++++ I +
Sbjct: 186 VSDAQHLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVE 245

Query: 249 GLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAGKPGN 308
            +       E +   L  +L++ +  K Y LVLDDVW+ ++  W  LR  LK   G  G 
Sbjct: 246 SIEGRPLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLK--VGASGA 305

Query: 309 TIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKELVRK 368
           +++ TTR E+V +++  +  Y L  LS + CW LF + A  +Q  +N  L  + KE+V+K
Sbjct: 306 SVLTTTRLEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKK 365

Query: 369 MGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKLSVDS 428
            GGVPL AK LGG + F+         E +W    +S + N+  +D+  +L  L+LS   
Sbjct: 366 SGGVPLAAKTLGGILCFKR-------EERAWEHVRDSPIWNLP-QDESSILPALRLSYHQ 425

Query: 429 LPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQYFNFL 488
           LP   LKQC AYC+ F +D   +K+ LI +W+A GF+        N+ +ED+G++ +  L
Sbjct: 426 LPLD-LKQCFAYCAVFPKDAKMEKEKLISLWMAHGFLL----SKGNMELEDVGDEVWKEL 485

Query: 489 LSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRTLICN 548
             RS FQ++     K    FKMHDL+HD+A ++ S     SN   ++  S   + ++   
Sbjct: 486 YLRSFFQEIEVKDGKTY--FKMHDLIHDLATSLFSANTSSSNIREINKHSYTHMMSIGFA 545

Query: 549 DEVINYL--NQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLESLSL 608
           + V  Y        + LRVL +   +   L   I  L+HLRYL++    + + L + L  
Sbjct: 546 EVVFFYTLPPLEKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGSGM-RSLPKQLCK 605

Query: 609 LYNLQTLKLGQSG----LPKNLRKLVNLRHLEFKMFGD-TAMPSDMGNLIHLQSLSGFLV 668
           L NLQTL L        LPK   KL +LR+L        T MP  +G+L  L++L  F+V
Sbjct: 606 LQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVV 665

Query: 669 GFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRG 728
           G +KG ++ ELG L NL G + +++L RV+N  +A  A L  K NL  L++ +       
Sbjct: 666 GRKKGYQLGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSWNNFGPHI 725

Query: 729 EDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGI---FVENLVKIRLGHFERCEVLP 788
            + E+  V+VLE L+PH NL SL+I GFRG  LP  +    ++N+V I + +F  C  LP
Sbjct: 726 YESEE--VKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLP 785

Query: 789 MLGQLPNLKELEIMY-MESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEA 848
             G LP L+ LE+ +    V  +      V S     + FP L+KL I++  +L+   + 
Sbjct: 786 PFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWDFGSLKGLLKK 845

Query: 849 TVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLE--YLSIRGCF--NLMLNVQNLHK 908
                   F  L+E+ I  C P   L S L    SL   Y  +   F   +  N+ NL  
Sbjct: 846 E---GEEQFPVLEEMIIHEC-PFLTLSSNLRALTSLRICYNKVATSFPEEMFKNLANLKY 905

Query: 909 LYHLEIDGLKRLPKGMDGLTRLKELKIGGC--MQNYEFSSVIHLASQLVELELSGRYGSV 967
           L     + LK LP  +  L  LK LKI  C  +++     +  L+S     EL   + ++
Sbjct: 906 LTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSL---TELFVEHCNM 939

BLAST of Cucsat.G12697 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 409.1 bits (1050), Expect = 1.5e-112
Identity = 341/1064 (32.05%), Postives = 537/1064 (50.47%), Query Frame = 0

Query: 9    VQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGNSVKRWVEK 68
            +Q +L  +  +   ++ + +  E E   L         +LED  ++K     ++K W++K
Sbjct: 6    LQVLLDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDA-QEKQLKYKAIKNWLQK 65

Query: 69   LEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMAKKIKNITDT 128
            L    +E DD+LD+   E  R       ++            +  F  K+ K++K + + 
Sbjct: 66   LNVAAYEVDDILDDCKTEAARFKQAVLGRYHP---------RTITFCYKVGKRMKEMMEK 125

Query: 129  LNQHYCAASAFGLVGVETVTEIELALNQIRETTSIL-DFQVEGREAEVLELLKLAIDSTN 188
            L+        F L   E + E + A    R+T  +L + +V GRE E  E++K+ I++ +
Sbjct: 126  LDAIAEERRNFHL--DERIIERQAAR---RQTGFVLTEPKVYGREKEEDEIVKILINNVS 185

Query: 189  -EHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILEKIFQGL 248
                + V+ I+GMGGLGKTTLA+M+FN + I  HF+  IWVCVS  F   ++++ I + +
Sbjct: 186  YSEEVPVLPILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESI 245

Query: 249  TKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAGKPGNTI 308
                 G + +   L  +L++ +  K YFLVLDDVW+ ++  WD LR  LK   G  G +I
Sbjct: 246  EGKSLG-DMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLK--IGASGASI 305

Query: 309  MVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKELVRKMG 368
            ++TTR E++ +++  + +Y+L  LS + CW LFK+ A  +Q   + KL  + KE+V+K G
Sbjct: 306  LITTRLEKIGSIMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKCG 365

Query: 369  GVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKLSVDSLP 428
            GVPL AK LGG ++F+  E E       W    +S + N+  +D++ VL  L+LS   LP
Sbjct: 366  GVPLAAKTLGGLLRFKREESE-------WEHVRDSEIWNLP-QDENSVLPALRLSYHHLP 425

Query: 429  NPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQYFNFLLS 488
               L+QC AYC+ F +D   +K+ LI +W+A  F+        N+ +ED+G + +N L  
Sbjct: 426  LD-LRQCFAYCAVFPKDTKIEKEYLIALWMAHSFLL----SKGNMELEDVGNEVWNELYL 485

Query: 489  RSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRTLICN-- 548
            RS FQ++   + K    FKMHDL+HD+A ++ S      +   ++ K    +  ++ N  
Sbjct: 486  RSFFQEIEVKSGKTY--FKMHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFIVTNYK 545

Query: 549  -------DEVINYLNQN---DIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISE---CS 608
                    EV++  + +     V LRVL +       L   +  L+HLRYLD+S    CS
Sbjct: 546  DMMSIGFSEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKICS 605

Query: 609  INKLLLESLSLLYNLQTLKL----GQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLI 668
            + K L +    L NLQTL L      S LPK   KL +LR+L       T+MP  +G L 
Sbjct: 606  LPKRLCK----LQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCPLTSMPPRIGLLT 665

Query: 669  HLQSLSGFLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLN 728
             L++L  F+VG  KG ++ EL  L NL+G +++T+L RV+N  EA  A L  K NL  L+
Sbjct: 666  CLKTLGYFVVGERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKANLHSLS 725

Query: 729  LWFFETDKRGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGI---FVENLVKIRL 788
            +  ++   R E +E   V+VLE L+PH NL+ LEI+ F G  LP  +    ++N+V I +
Sbjct: 726  M-SWDRPNRYESEE---VKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILI 785

Query: 789  GHFERCEVLPMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEM 848
               E C  LP  G+LP L+ LE+       S+  E Y  DS       FP L+KL I   
Sbjct: 786  SGCENCSCLPPFGELPCLESLEL----QDGSVEVE-YVEDSGFLTRRRFPSLRKLHIGGF 845

Query: 849  MNLEQWDEATVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNV 908
             NL+       +  +  F  L+E++I  C P+   P+                   + +V
Sbjct: 846  CNLKGLQR---MKGAEQFPVLEEMKISDC-PMFVFPT-------------------LSSV 905

Query: 909  QNLHKLYHLEIDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVE--LELSG 968
            + L      +  GL      +  L+ L  LKI     N+  +S++    + +E  + LS 
Sbjct: 906  KKLEIWGEADAGGL----SSISNLSTLTSLKI---FSNHTVTSLLEEMFKNLENLIYLSV 965

Query: 969  RYGSVDTQLPQQLQHLTNLQVLKITQFDCIEALP-EWIGNLISLKTLKCSYCFKLKELPS 1028
             +     +LP  L  L NL+ L I     +E+LP E +  L SL  L   +C  LK LP 
Sbjct: 966  SFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEHCNMLKCLP- 987

Query: 1029 REAILRLTKLENLDIFECPKLL------VGEGDQERAKLSHLPS 1040
             E +  LT L +L I  CP+L+      +GE   +  K+SH+P+
Sbjct: 1026 -EGLQHLTTLTSLKIRGCPQLIKRCEKGIGE---DWHKISHIPN 987

BLAST of Cucsat.G12697 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 406.8 bits (1044), Expect = 7.7e-112
Identity = 344/1059 (32.48%), Postives = 524/1059 (49.48%), Query Frame = 0

Query: 9    VQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGNSVKRWVEK 68
            +Q VL  +  +   ++V+ +  ++E   L         +LED   K+ +    ++ W++K
Sbjct: 6    IQVVLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLN-DKPLENWLQK 65

Query: 69   LEDIVHEADDLLDELVYEHLR-RTVEHTEKFSKVSDSISSSINSFLFRRKMAKKIKNITD 128
            L    +E DD+LDE   +  R    E+     KV            FR K+ K++  +  
Sbjct: 66   LNAATYEVDDILDEYKTKATRFLQSEYGRYHPKV----------IPFRHKVGKRMDQVMK 125

Query: 129  TLNQHYCAASAFGLVGVETVTEIELALNQIRETTSIL-DFQVEGREAEVLELLKLAIDST 188
             LN        F L   E + E + A    RET S+L + QV GR+ E  E++K+ I++ 
Sbjct: 126  KLNAIAEERKKFHL--QEKIIERQAA---TRETGSVLTEPQVYGRDKEKDEIVKILINTA 185

Query: 189  NE-HHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILEKIFQG 248
            ++   +SV+ I+GMGGLGKTTL++M+FN + +   F   IW+C+S  F   ++++ I + 
Sbjct: 186  SDAQKLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVES 245

Query: 249  LT-KTCSGLESNKEALLGRLRKEMQD----KNYFLVLDDVWDNEKHLWDELRGCLKHIAG 308
            +  K+ S ++      L  L+K++Q+    K YFLVLDDVW+ ++H W  LR  LK   G
Sbjct: 246  IEGKSLSDMD------LAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLK--VG 305

Query: 309  KPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKE 368
              G  ++ TTR E+V +++  +  Y L  LS + CW LF + A  +Q  +N  L  + KE
Sbjct: 306  ASGAFVLTTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKE 365

Query: 369  LVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKL 428
            +V+K GGVPL AK LGG ++F+  E E       W    +S + N+  +D+  +L  L+L
Sbjct: 366  IVKKCGGVPLAAKTLGGILRFKREERE-------WEHVRDSPIWNLP-QDESSILPALRL 425

Query: 429  SVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQY 488
            S   LP   L+QC  YC+ F +D    K++LI  W+A GF+        NL +ED+G + 
Sbjct: 426  SYHHLPLD-LRQCFVYCAVFPKDTKMAKENLIAFWMAHGFLL----SKGNLELEDVGNEV 485

Query: 489  FNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRT 548
            +N L  RS FQ++  ++ K    FKMHDL+HD+A ++ S     SN   ++      + +
Sbjct: 486  WNELYLRSFFQEIEVESGKTY--FKMHDLIHDLATSLFSANTSSSNIREINANYDGYMMS 545

Query: 549  LICNDEVINYLNQ--NDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLE 608
            +   + V +Y        V LRVL +   +   L   I  L+HLRYLD+S     + L +
Sbjct: 546  IGFAEVVSSYSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPK 605

Query: 609  SLSLLYNLQTLKL----GQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSG 668
             L  L NLQTL L      S LPK   KL +LR+L       T+ P  +G L  L+SLS 
Sbjct: 606  RLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLTSTPPRIGLLTCLKSLSC 665

Query: 669  FLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETD 728
            F++G  KG ++ EL  L NL G +++T L RV+   +A  A L  K NL  L L +    
Sbjct: 666  FVIGKRKGHQLGELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLSWDLDG 725

Query: 729  KRGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGI---FVENLVKIRLGHFERCE 788
            K   D E     VLE L+PH NL+ LEI GF G  LP  +    ++N+V IR+   E C 
Sbjct: 726  KHRYDSE-----VLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENCS 785

Query: 789  VLPMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWD 848
             LP  G+LP L+ LE+          +  Y  D+ H     FP L+KL I++  NL    
Sbjct: 786  CLPPFGELPCLESLELH-----TGSADVEYVEDNVHPG--RFPSLRKLVIWDFSNL---- 845

Query: 849  EATVVLE-SNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKL 908
            +  + +E    F  L+E+    C P+  +P+ L    +L+ +       ++ ++ NL  L
Sbjct: 846  KGLLKMEGEKQFPVLEEMTFYWC-PMFVIPT-LSSVKTLKVIVTDA--TVLRSISNLRAL 905

Query: 909  YHLEIDG---LKRLPKGM-DGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGS 968
              L+I        LP+ M   L  LK LKI    +N +                      
Sbjct: 906  TSLDISDNVEATSLPEEMFKSLANLKYLKI-SFFRNLK---------------------- 965

Query: 969  VDTQLPQQLQHLTNLQVLKITQFDCIEALP-EWIGNLISLKTLKCSYCFKLKELPSREAI 1028
               +LP  L  L  L+ LK    D +E+LP E +  L SL  L  S C  LK LP  E +
Sbjct: 966  ---ELPTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLP--EGL 973

Query: 1029 LRLTKLENLDIFECPKLL------VGEGDQERAKLSHLP 1039
              LT L  L I +CP +       +GE   +  K++H+P
Sbjct: 1026 QHLTALTTLTITQCPIVFKRCERGIGE---DWHKIAHIP 973

BLAST of Cucsat.G12697 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 3.6e-109
Identity = 339/1060 (31.98%), Postives = 527/1060 (49.72%), Query Frame = 0

Query: 9    VQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGNSVKRWVEK 68
            +Q +L+ +  +  +++V+ +  E E   L         +L+D  ++K     +++ W++K
Sbjct: 6    LQVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDA-QEKQLKDKAIENWLQK 65

Query: 69   LEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMAKKIKNITDT 128
            L    +E DD+L E   E +R       + S++       IN   FR K+ +++K I + 
Sbjct: 66   LNSAAYEVDDILGECKNEAIR------FEQSRLGFYHPGIIN---FRHKIGRRMKEIMEK 125

Query: 129  LNQHYCAASAFGLVGVETVTEIELALNQIRETTSIL-DFQVEGREAEVLELLKLAIDSTN 188
            L+        F    +E +TE + A    RET  +L + +V GR+ E  E++K+ I++ N
Sbjct: 126  LDAISEERRKFHF--LEKITERQAAA-ATRETGFVLTEPKVYGRDKEEDEIVKILINNVN 185

Query: 189  -EHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILEKIFQGL 248
                + V  I+GMGGLGKTTLA+MIFN   +  HF+  IWVCVS  F   ++++ I   +
Sbjct: 186  VAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNI 245

Query: 249  TKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAGKPGNTI 308
             ++   +E +  +   +L++ +  K Y LVLDDVW+++   W +LR  L    G  G +I
Sbjct: 246  ERSSPHVE-DLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVL--TVGARGASI 305

Query: 309  MVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKELVRKMG 368
            + TTR E+V +++  +  Y L  LS      LF + A   Q   N  L  + KE+V+K G
Sbjct: 306  LATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEIVKKCG 365

Query: 369  GVPLVAKVLGGAVKFEETELEEE---DHEISWMTKVESIVRNISLEDKDFVLSILKLSVD 428
            GVPL AK LGG ++F+  E E E   D+EI W             +D+  +L  L+LS  
Sbjct: 366  GVPLAAKTLGGLLRFKREESEWEHVRDNEI-WSLP----------QDESSILPALRLSYH 425

Query: 429  SLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQYFNF 488
             LP   L+QC AYC+ F +D    K++LI +W+A GF+        NL +ED+G + +N 
Sbjct: 426  HLPLD-LRQCFAYCAVFPKDTKMIKENLITLWMAHGFLL----SKGNLELEDVGNEVWNE 485

Query: 489  LLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRTLIC 548
            L  RS FQ++  +A      FK+HDL+HD+A ++ S      N   ++ K  +   ++  
Sbjct: 486  LYLRSFFQEI--EAKSGNTYFKIHDLIHDLATSLFSASASCGNIREINVKDYKHTVSIGF 545

Query: 549  NDEVINYLNQ--NDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLESLS 608
               V +Y        V LRVL + +     L   I  L+HLRYLD+S C+  + L E L 
Sbjct: 546  AAVVSSYSPSLLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLS-CNNFRSLPERLC 605

Query: 609  LLYNLQTLKL----GQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLV 668
             L NLQTL +      + LPK   KL +LRHL       T+ P  +G L  L++L  F+V
Sbjct: 606  KLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPRIGLLTCLKTLGFFIV 665

Query: 669  GFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNL-WFFETDKR 728
            G +KG ++ EL  L NL G +++T+L RV+N  +A  A L  K NL+ L++ W  +   R
Sbjct: 666  GSKKGYQLGELKNL-NLCGSISITHLERVKNDTDA-EANLSAKANLQSLSMSWDNDGPNR 725

Query: 729  GEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGI---FVENLVKIRLGHFERCEVL 788
             E  E   V+VLE L+PH NL+ LEI+ F G   P+ I    +E ++ +R+   + C  L
Sbjct: 726  YESKE---VKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCLCL 785

Query: 789  PMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEA 848
            P  G+LP L+ LE+    +      E   V S      +FP LKKL I+   +L+     
Sbjct: 786  PPFGELPCLENLELQNGSAEVEYVEE-DDVHSRFSTRRSFPSLKKLRIWFFRSLK----- 845

Query: 849  TVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGC----FNLMLNVQNLHK 908
                     G +KE              G E    LE ++I  C    F  + +V+ L  
Sbjct: 846  ---------GLMKE-------------EGEEKFPMLEEMAILYCPLFVFPTLSSVKKLEV 905

Query: 909  LYHLEIDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSV-IHLASQLVELELSGRYGSVD 968
              +    GL      +  L+ L  L+IG    NY  +S+   + + L  LE    +   +
Sbjct: 906  HGNTNTRGL----SSISNLSTLTSLRIGA---NYRATSLPEEMFTSLTNLEFLSFFDFKN 965

Query: 969  TQ-LPQQLQHLTNLQVLKITQFDCIEALPE-WIGNLISLKTLKCSYCFKLKELPSREAIL 1028
             + LP  L  L  L+ L+I   D +E+ PE  +  L SL  L   YC  LK LP  E + 
Sbjct: 966  LKDLPTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLP--EGLQ 988

Query: 1029 RLTKLENLDIFECP---KLLVGEGDQERAKLSHLPSKCVH 1044
             LT L NL +  CP   K    E  ++  K++H+P+  +H
Sbjct: 1026 HLTALTNLGVSGCPEVEKRCDKEIGEDWHKIAHIPNLDIH 988

BLAST of Cucsat.G12697 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 4.4e-91
Identity = 271/904 (29.98%), Postives = 464/904 (51.33%), Query Frame = 0

Query: 31  ENEVSLLKDKLHDADTILEDINRKK-SHPGNSVKRWVEKLEDIVHEADDLLDELVYEHLR 90
           EN +  L   L     +L D   K+ ++P   V++WV +L D+V+ A+D LD++  E LR
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNP--VVEKWVNELRDVVYHAEDALDDIATEALR 95

Query: 91  RTVEHTEKFSKVSDSISS--SINSFL--FRRKMAKKIKNITDTLNQHYCAASAFGLVGVE 150
             +      S     +    S+  FL      +  +++ +T  L +    AS   ++G++
Sbjct: 96  LNIGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLER---LASQRNILGLK 155

Query: 151 TVTEIELALNQIRETTSILD-FQVEGREAEVLELLKLAI-DSTNEHHMSVISIVGMGGLG 210
            +T   +   Q   TTS++D  +V GR+ +  E+++  I ++  ++ ++V++IVG+GG+G
Sbjct: 156 ELT--AMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVG 215

Query: 211 KTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILEKIFQGLT-KTCSGLESNKEALLG 270
           KTTL+++++N + +  +F   +W  VS+ F V KI +K+++ +T + C    ++ + L  
Sbjct: 216 KTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCE--FTDLDVLQV 275

Query: 271 RLRKEMQDKN--YFLVLDDVWDNEKHLWDELRGCLKHIAGKPGNTIMVTTRNEEVATMVE 330
           +L++ +      + LVLDD+W+     WD LR    H A   G+ I+VTTR++ VA+++ 
Sbjct: 276 KLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAA--QGSQILVTTRSQRVASIMC 335

Query: 331 PISIYRLKKLSNDQCWALFKESANANQLP-MNSKLEIMKKELVRKMGGVPLVAKVLGGAV 390
            + ++ L+ LS+  CW+LF ++   NQ P +N ++  + + +V K  G+PL  K LGG +
Sbjct: 336 AVHVHNLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVL 395

Query: 391 KFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKLSVDSLPNPVLKQCVAYCSN 450
           +FE   +E       W   + S + ++   DK  +L +L++S   LP   LK+C AYCS 
Sbjct: 396 RFEGKVIE-------WERVLSSRIWDLP-ADKSNLLPVLRVSYYYLP-AHLKRCFAYCSI 455

Query: 451 FSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQYFNFLLSRSIFQDVTRDANK 510
           F + + F+KD ++ +W+A+GF+Q  +   KNL  E++G +YF+ L SRS+ Q       K
Sbjct: 456 FPKGHAFEKDKVVLLWMAEGFLQQTRS-SKNL--EELGNEYFSELESRSLLQ-------K 515

Query: 511 RIVGFKMHDLMHDIA--------------CAISSHQNVE---------SNPNNLSG-KSV 570
               + MHD ++++A              C +   +            + P      + V
Sbjct: 516 TKTRYIMHDFINELAQFASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREV 575

Query: 571 RKLRTLI-----------CNDEVINYLNQNDIVCLRVLKVIFQSHTDL-WIPID---KLI 630
           + LRT +           C D+++   ++  +  L  L+V+  SH  +  +P D    + 
Sbjct: 576 KFLRTFLPLSLTNSSRSCCLDQMV---SEKLLPTLTRLRVLSLSHYKIARLPPDFFKNIS 635

Query: 631 HLRYLDISECSINKLLLESLSLLYNLQTLKLGQSG----LPKNLRKLVNLRHLEFKMFGD 690
           H R+LD+S   + K L +SL  +YNLQTL L        LP ++  L+NLR+L+      
Sbjct: 636 HARFLDLSRTELEK-LPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKL 695

Query: 691 TAMPSDMGNLIHLQSLSGFLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAK 750
             MP   G L  LQ+L+ F V    G +I ELG L +L GKL +  L RV +  +A  A 
Sbjct: 696 RQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEAN 755

Query: 751 LVEKKNLRHLNLWFFETDKRGEDDED-----GIVQVLEGLQPHKNLQSLEILGFRGKVLP 810
           L  KK+LR ++  +       E++ +        +V E L+PH++++ L I  ++G+  P
Sbjct: 756 LNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFP 815

Query: 811 TGI---FVENLVKIRLGHFERCEVLPMLGQLPNLKELEIMYMESVRSIGNEFYGVDSS-- 870
             +       +V IRL   + C  LP LGQLP LKEL I  M  ++SIG +FY  D    
Sbjct: 816 DWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLR 875

BLAST of Cucsat.G12697 vs. NCBI nr
Match: XP_004136783.2 (disease resistance protein RGA2 [Cucumis sativus])

HSP 1 Score: 2099 bits (5438), Expect = 0.0
Identity = 1053/1053 (100.00%), Postives = 1053/1053 (100.00%), Query Frame = 0

Query: 1    MAEIGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGN 60
            MAEIGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGN
Sbjct: 1    MAEIGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGN 60

Query: 61   SVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMAK 120
            SVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMAK
Sbjct: 61   SVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMAK 120

Query: 121  KIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELLK 180
            KIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELLK
Sbjct: 121  KIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELLK 180

Query: 181  LAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILE 240
            LAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILE
Sbjct: 181  LAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILE 240

Query: 241  KIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAG 300
            KIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAG
Sbjct: 241  KIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAG 300

Query: 301  KPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKE 360
            KPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKE
Sbjct: 301  KPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKE 360

Query: 361  LVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKL 420
            LVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKL
Sbjct: 361  LVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKL 420

Query: 421  SVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQY 480
            SVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQY
Sbjct: 421  SVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQY 480

Query: 481  FNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRT 540
            FNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRT
Sbjct: 481  FNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRT 540

Query: 541  LICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLESL 600
            LICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLESL
Sbjct: 541  LICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLESL 600

Query: 601  SLLYNLQTLKLGQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLVGFE 660
            SLLYNLQTLKLGQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLVGFE
Sbjct: 601  SLLYNLQTLKLGQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLVGFE 660

Query: 661  KGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGEDD 720
            KGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGEDD
Sbjct: 661  KGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGEDD 720

Query: 721  EDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLP 780
            EDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLP
Sbjct: 721  EDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLP 780

Query: 781  NLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESN 840
            NLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESN
Sbjct: 781  NLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESN 840

Query: 841  LFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKR 900
            LFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKR
Sbjct: 841  LFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKR 900

Query: 901  LPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQHLTN 960
            LPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQHLTN
Sbjct: 901  LPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQHLTN 960

Query: 961  LQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIFECP 1020
            LQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIFECP
Sbjct: 961  LQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIFECP 1020

Query: 1021 KLLVGEGDQERAKLSHLPSKCVHKSESFYSGLL 1053
            KLLVGEGDQERAKLSHLPSKCVHKSESFYSGLL
Sbjct: 1021 KLLVGEGDQERAKLSHLPSKCVHKSESFYSGLL 1053

BLAST of Cucsat.G12697 vs. NCBI nr
Match: KAE8651192.1 (hypothetical protein Csa_002145 [Cucumis sativus])

HSP 1 Score: 1875 bits (4858), Expect = 0.0
Identity = 936/936 (100.00%), Postives = 936/936 (100.00%), Query Frame = 0

Query: 118  MAKKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLE 177
            MAKKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLE
Sbjct: 1    MAKKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLE 60

Query: 178  LLKLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTK 237
            LLKLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTK
Sbjct: 61   LLKLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTK 120

Query: 238  ILEKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKH 297
            ILEKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKH
Sbjct: 121  ILEKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKH 180

Query: 298  IAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIM 357
            IAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIM
Sbjct: 181  IAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIM 240

Query: 358  KKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSI 417
            KKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSI
Sbjct: 241  KKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSI 300

Query: 418  LKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIG 477
            LKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIG
Sbjct: 301  LKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIG 360

Query: 478  EQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRK 537
            EQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRK
Sbjct: 361  EQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRK 420

Query: 538  LRTLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLL 597
            LRTLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLL
Sbjct: 421  LRTLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLL 480

Query: 598  ESLSLLYNLQTLKLGQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLV 657
            ESLSLLYNLQTLKLGQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLV
Sbjct: 481  ESLSLLYNLQTLKLGQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLV 540

Query: 658  GFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRG 717
            GFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRG
Sbjct: 541  GFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRG 600

Query: 718  EDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLG 777
            EDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLG
Sbjct: 601  EDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLG 660

Query: 778  QLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVL 837
            QLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVL
Sbjct: 661  QLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVL 720

Query: 838  ESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDG 897
            ESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDG
Sbjct: 721  ESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDG 780

Query: 898  LKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQH 957
            LKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQH
Sbjct: 781  LKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQH 840

Query: 958  LTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIF 1017
            LTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIF
Sbjct: 841  LTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIF 900

Query: 1018 ECPKLLVGEGDQERAKLSHLPSKCVHKSESFYSGLL 1053
            ECPKLLVGEGDQERAKLSHLPSKCVHKSESFYSGLL
Sbjct: 901  ECPKLLVGEGDQERAKLSHLPSKCVHKSESFYSGLL 936

BLAST of Cucsat.G12697 vs. NCBI nr
Match: XP_004140442.1 (disease resistance protein RGA2 [Cucumis sativus] >KGN50811.1 hypothetical protein Csa_018865 [Cucumis sativus])

HSP 1 Score: 1362 bits (3525), Expect = 0.0
Identity = 733/1049 (69.88%), Postives = 831/1049 (79.22%), Query Frame = 0

Query: 1    MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60
            MAE + TF VQEVLK+IV +GAEQI +AW LE E+S LK  L  A TIL DIN KKSH  
Sbjct: 1    MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHH- 60

Query: 61   NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMA 120
            +SV  WVE+L DI++EADDLLDE+VYE +R+TVE T K  KV DSIS S NSFLF  KMA
Sbjct: 61   HSVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLRKVRDSISPSKNSFLFGLKMA 120

Query: 121  KKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELL 180
            KK+K IT TL +HYC AS  GLVG E+ TE E ALNQIRETTSILDF+VEGREAEVLE+L
Sbjct: 121  KKMKKITKTLYEHYCEASPLGLVGDESTTESEAALNQIRETTSILDFEVEGREAEVLEIL 180

Query: 181  KLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKIL 240
            KL IDST+E H+SVISIVGMGGLGKTTLAKM+FNH  I+GHFDKT+WVCVSKPFIV KIL
Sbjct: 181  KLVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVMKIL 240

Query: 241  EKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIA 300
            E IFQGLT T SGL S +EALL RLR+EMQ K YFLVLDDVWD E  LWDEL G LK+IA
Sbjct: 241  EAIFQGLTNTSSGLNS-REALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKYIA 300

Query: 301  GKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKK 360
            GK GN+IMVTTR+ EVATMV+ + IY LKKLS+D CWAL K+SANANQL MNSKLE  K 
Sbjct: 301  GKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANANQLQMNSKLENTKN 360

Query: 361  ELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILK 420
             LVRK+GGVPL+AKVLGGAVKFEE   E      SWM K+ES  RNIS+EDKDFVLSILK
Sbjct: 361  ILVRKIGGVPLIAKVLGGAVKFEEGGSE------SWMAKIESFARNISIEDKDFVLSILK 420

Query: 421  LSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQ 480
            LSV+SLP+  LKQC AYCSNF QDY+F KD+ I+MWIA+GFIQP Q R+ NL ME+IGE+
Sbjct: 421  LSVESLPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERE-NLTMENIGEE 480

Query: 481  YFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLR 540
            Y NFLLSRS+F+D  +  + RIV FK+HDLMHDIACAIS+H  ++SNP + +GKS RKLR
Sbjct: 481  YLNFLLSRSLFEDAIK-YDGRIVTFKIHDLMHDIACAISNHHKMDSNPISWNGKSTRKLR 540

Query: 541  TLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLES 600
            TLIC +E   +  Q DI+CLRVL + +     L   +DKLIHLRYLDIS C+INKLL +S
Sbjct: 541  TLICENEEAFHKIQTDIICLRVLVLKWFDTNTLSTIMDKLIHLRYLDISNCNINKLLRDS 600

Query: 601  LSLLYNLQTLKLG--QSGLPKNLRKLVNLRHLEFKMFGDTA-MPSDMGNLIHLQSLSGFL 660
            +  LYNLQTLKLG  +  LPKNLR LVNLRHLEFK F D   MPS MGN+IHLQ+LS F+
Sbjct: 601  ICALYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFV 660

Query: 661  VGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNL-WFFETDK 720
            VG EKGCKI+ELGPLK+LKG LTL NL  VQNKDEAMAAKLVEKK LRHL   WF     
Sbjct: 661  VGLEKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFLNLYD 720

Query: 721  RGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPM 780
            RGE DED   QVLEGLQPHKN+QSL+I GF+G+VL   IFVENLV+IRL    RCEVLPM
Sbjct: 721  RGEYDEDDNKQVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLPM 780

Query: 781  LGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATV 840
            LGQLPNLK+LEI+ M SVRSIG+EFYGVD + +NS AFPQL K  I  +  L+QWDEATV
Sbjct: 781  LGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKLQQWDEATV 840

Query: 841  VLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEI 900
               SN FGCLKE+ +  C+ LAKLPSGLEGC+S+EYL+I GC NLMLNVQNL+ LYHL+I
Sbjct: 841  -FASNRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLDI 900

Query: 901  DGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELS--GRYGSVDTQLPQ 960
             GLKRLP     LT LK+L+IGGCMQNYEFS  IHL+SQLVELEL+  G  GS  TQLPQ
Sbjct: 901  RGLKRLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSGSETTQLPQ 960

Query: 961  QLQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLEN 1020
            QLQHLTNL+VLKI  FD IE LPEW+GNL  L TL    C  LKELPSREAI RLTKL++
Sbjct: 961  QLQHLTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAIQRLTKLDD 1020

Query: 1021 LDIFECPKLLVGEGDQERAKLSHLPSKCV 1042
            L I  CPKLL+GEGDQERAKLSHLPSKCV
Sbjct: 1021 LVIDGCPKLLLGEGDQERAKLSHLPSKCV 1038

BLAST of Cucsat.G12697 vs. NCBI nr
Match: XP_038877689.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 1326 bits (3431), Expect = 0.0
Identity = 730/1104 (66.12%), Postives = 832/1104 (75.36%), Query Frame = 0

Query: 1    MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60
            MAE + TF VQE+LK+IVK+GAEQI VAW LE E   LK  L  A+TIL DIN+KK H  
Sbjct: 1    MAEFLWTFAVQEILKKIVKFGAEQIGVAWGLEKEEFQLKKWLLKAETILADINKKKLHH- 60

Query: 61   NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMA 120
            ++V+ WVE L+DIV+EADDLLDEL YE LRRT E T KF KV DSIS S NSFLFR KMA
Sbjct: 61   HAVRLWVEDLQDIVYEADDLLDELRYEDLRRTGEQTGKFKKVRDSISPSKNSFLFRLKMA 120

Query: 121  KKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELL 180
             K+K IT  L++HYC AS  GLVG E+ TEIE+AL QIRETTSILDF+V GRE EVLE+L
Sbjct: 121  NKMKKITAALHKHYCEASPLGLVGEESATEIEVALQQIRETTSILDFEVVGREDEVLEIL 180

Query: 181  KLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKIL 240
            KL IDS+NEH MS+ISIVGMGGLGKTTLAKM+FNH  I+GHFD+TIWVCVSKPFIV KIL
Sbjct: 181  KLVIDSSNEHDMSMISIVGMGGLGKTTLAKMVFNHDAIKGHFDQTIWVCVSKPFIVMKIL 240

Query: 241  EKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIA 300
            E IFQ LT + SGL S KEALL RLRKEMQ K YFLVLDDVW+ E  LWDEL G LK IA
Sbjct: 241  EAIFQSLTNSSSGLNS-KEALLQRLRKEMQSKKYFLVLDDVWEKENDLWDELNGYLKQIA 300

Query: 301  GKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKK 360
            GK GN+IMVTTRN EVATMV+ +SI+ L+ LS+D CW+LF++ ANA+QLPMNS  +IMK 
Sbjct: 301  GKSGNSIMVTTRNVEVATMVKTVSIHHLQTLSDDNCWSLFEKCANASQLPMNSNFKIMKN 360

Query: 361  ELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILK 420
             LVRK+GGVPLVAKVLGGA+KFE       DH   WMTKVESI RNI  ED+ FVLSIL+
Sbjct: 361  MLVRKIGGVPLVAKVLGGALKFEG------DHYERWMTKVESITRNILKEDEAFVLSILR 420

Query: 421  LSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQ 480
            LSVDSLP   LKQC AYCSNF QDY+F+KD+LI+MWIAQGFIQP + R+ NL+MEDIGE+
Sbjct: 421  LSVDSLPLLTLKQCFAYCSNFPQDYNFKKDELIQMWIAQGFIQPQEERE-NLIMEDIGEE 480

Query: 481  YFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLR 540
            YFNFLLSRS+FQDV +D N RIV FKMHDL+HDIAC +S+HQ   SNPNN SGKS R LR
Sbjct: 481  YFNFLLSRSLFQDVDKDENGRIVTFKMHDLIHDIACMVSNHQKKASNPNNWSGKSARTLR 540

Query: 541  TLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLES 600
             LICN+E   +  Q +I CLRVL +      +L I +DKLIHLRYLDIS+CS+N+L  ES
Sbjct: 541  ALICNNEETPHKIQANINCLRVLVLNSFETNNLSIIMDKLIHLRYLDISKCSVNQLSQES 600

Query: 601  LSLLYNLQTLKLGQSG--LPKNLRKLVNLRHLEF-KMFGDTAMPSDMGNLIHLQSLSGFL 660
            LS LYNLQTLK G  G  LPKNLRKLVNLRHLEF K F    M S MGNLIHLQ+LS F+
Sbjct: 601  LSSLYNLQTLKFGYIGSDLPKNLRKLVNLRHLEFQKKFDMKQMASHMGNLIHLQTLSEFV 660

Query: 661  VGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNL-WFFETDK 720
            VG  K  KIEELGPLKNLKG L +TNL +VQNK+EA+AAKLVEKKNLRHL L WF     
Sbjct: 661  VGLGKAYKIEELGPLKNLKGTLIITNLEKVQNKEEAIAAKLVEKKNLRHLILKWFVGLYN 720

Query: 721  RGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPM 780
            RGE  ED  V+VLEGLQPHKNLQSLEI GF+GKV P  IFV+NLV++ L   ERC +LPM
Sbjct: 721  RGEYYEDDHVRVLEGLQPHKNLQSLEINGFKGKVFPNNIFVQNLVEVDLYKCERCVMLPM 780

Query: 781  LGQLPNLKELEIMYMESVRSIGNEFYGVDSSH-QNSVAFPQLKKLSIYEMMNLEQWDEAT 840
            LGQLPNLK+L I  M SV+SIGNEFYG+DS+  +NS AFPQLK+L IY M  LEQWDE+T
Sbjct: 781  LGQLPNLKKLLIRSMVSVKSIGNEFYGIDSNQLRNSFAFPQLKELHIYGMKKLEQWDEST 840

Query: 841  VVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLE 900
            VV  SNLFGCLK++ I  C  LAKLPSGLEGCHS+EYL I  C NLMLNVQN + LYHL+
Sbjct: 841  VV-SSNLFGCLKKLHISGCGQLAKLPSGLEGCHSIEYLGIFQCSNLMLNVQNFYNLYHLD 900

Query: 901  IDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVD----TQ 960
            I+GLKRLP+G+  LT LK L+I G + NYEFSS IHL+SQLVELEL+  YGS D    TQ
Sbjct: 901  INGLKRLPEGLGKLTNLKILRISGQLHNYEFSSFIHLSSQLVELELND-YGSSDNATTTQ 960

Query: 961  LPQQLQHLTNLQVLKI-------------------------------------------- 1020
            LPQQLQ+LTNL++L+I                                            
Sbjct: 961  LPQQLQYLTNLKILRIGGCMQNYEFSSFRHLPTQLVKLELIDDESSDDATTQLPQFQHFT 1020

Query: 1021 -------TQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIFEC 1043
                   + FD IE LP+W+GNL SL+TL    C  LKELPSREAIL LTKLENL +F C
Sbjct: 1021 NLNVLVISNFDGIETLPDWLGNLTSLRTLHFFECKNLKELPSREAILHLTKLENLVVFRC 1080

BLAST of Cucsat.G12697 vs. NCBI nr
Match: XP_031741283.1 (disease resistance protein RGA2-like [Cucumis sativus])

HSP 1 Score: 1237 bits (3201), Expect = 0.0
Identity = 657/931 (70.57%), Postives = 743/931 (79.81%), Query Frame = 0

Query: 118  MAKKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLE 177
            MAKK+K IT TL +HYC AS  GLVG E+ TE E ALNQIRETTSILDF+VEGREAEVLE
Sbjct: 1    MAKKMKKITKTLYEHYCEASPLGLVGDESTTESEAALNQIRETTSILDFEVEGREAEVLE 60

Query: 178  LLKLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTK 237
            +LKL IDST+E H+SVISIVGMGGLGKTTLAKM+FNH  I+GHFDKT+WVCVSKPFIV K
Sbjct: 61   ILKLVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVMK 120

Query: 238  ILEKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKH 297
            ILE IFQGLT T SGL S +EALL RLR+EMQ K YFLVLDDVWD E  LWDEL G LK+
Sbjct: 121  ILEAIFQGLTNTSSGLNS-REALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKY 180

Query: 298  IAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIM 357
            IAGK GN+IMVTTR+ EVATMV+ + IY LKKLS+D CWAL K+SANANQL MNSKLE  
Sbjct: 181  IAGKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANANQLQMNSKLENT 240

Query: 358  KKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSI 417
            K  LVRK+GGVPL+AKVLGGAVKFEE   E      SWM K+ES  RNIS+EDKDFVLSI
Sbjct: 241  KNILVRKIGGVPLIAKVLGGAVKFEEGGSE------SWMAKIESFARNISIEDKDFVLSI 300

Query: 418  LKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIG 477
            LKLSV+SLP+  LKQC AYCSNF QDY+F KD+ I+MWIA+GFIQP Q R+ NL ME+IG
Sbjct: 301  LKLSVESLPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERE-NLTMENIG 360

Query: 478  EQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRK 537
            E+Y NFLLSRS+F+D  +  + RIV FK+HDLMHDIACAIS+H  ++SNP + +GKS RK
Sbjct: 361  EEYLNFLLSRSLFEDAIK-YDGRIVTFKIHDLMHDIACAISNHHKMDSNPISWNGKSTRK 420

Query: 538  LRTLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLL 597
            LRTLIC +E   +  Q DI+CLRVL + +     L   +DKLIHLRYLDIS C+INKLL 
Sbjct: 421  LRTLICENEEAFHKIQTDIICLRVLVLKWFDTNTLSTIMDKLIHLRYLDISNCNINKLLR 480

Query: 598  ESLSLLYNLQTLKLG--QSGLPKNLRKLVNLRHLEFKMFGDTA-MPSDMGNLIHLQSLSG 657
            +S+  LYNLQTLKLG  +  LPKNLR LVNLRHLEFK F D   MPS MGN+IHLQ+LS 
Sbjct: 481  DSICALYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSE 540

Query: 658  FLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNL-WFFET 717
            F+VG EKGCKI+ELGPLK+LKG LTL NL  VQNKDEAMAAKLVEKK LRHL   WF   
Sbjct: 541  FVVGLEKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFLNL 600

Query: 718  DKRGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVL 777
              RGE DED   QVLEGLQPHKN+QSL+I GF+G+VL   IFVENLV+IRL    RCEVL
Sbjct: 601  YDRGEYDEDDNKQVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVL 660

Query: 778  PMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEA 837
            PMLGQLPNLK+LEI+ M SVRSIG+EFYGVD + +NS AFPQL K  I  +  L+QWDEA
Sbjct: 661  PMLGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKLQQWDEA 720

Query: 838  TVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHL 897
            TV   SN FGCLKE+ +  C+ LAKLPSGLEGC+S+EYL+I GC NLMLNVQNL+ LYHL
Sbjct: 721  TV-FASNRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHL 780

Query: 898  EIDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELS--GRYGSVDTQL 957
            +I GLKRLP     LT LK+L+IGGCMQNYEFS  IHL+SQLVELEL+  G  GS  TQL
Sbjct: 781  DIRGLKRLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSGSETTQL 840

Query: 958  PQQLQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKL 1017
            PQQLQHLTNL+VLKI  FD IE LPEW+GNL  L TL    C  LKELPSREAI RLTKL
Sbjct: 841  PQQLQHLTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAIQRLTKL 900

Query: 1018 ENLDIFECPKLLVGEGDQERAKLSHLPSKCV 1042
            ++L I  CPKLL+GEGDQERAKLSHLPSKCV
Sbjct: 901  DDLVIDGCPKLLLGEGDQERAKLSHLPSKCV 921

BLAST of Cucsat.G12697 vs. ExPASy TrEMBL
Match: A0A0A0LBV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1)

HSP 1 Score: 2099 bits (5438), Expect = 0.0
Identity = 1053/1053 (100.00%), Postives = 1053/1053 (100.00%), Query Frame = 0

Query: 1    MAEIGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGN 60
            MAEIGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGN
Sbjct: 1    MAEIGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPGN 60

Query: 61   SVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMAK 120
            SVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMAK
Sbjct: 61   SVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMAK 120

Query: 121  KIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELLK 180
            KIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELLK
Sbjct: 121  KIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELLK 180

Query: 181  LAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILE 240
            LAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILE
Sbjct: 181  LAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKILE 240

Query: 241  KIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAG 300
            KIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAG
Sbjct: 241  KIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIAG 300

Query: 301  KPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKE 360
            KPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKE
Sbjct: 301  KPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKKE 360

Query: 361  LVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKL 420
            LVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKL
Sbjct: 361  LVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILKL 420

Query: 421  SVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQY 480
            SVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQY
Sbjct: 421  SVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQY 480

Query: 481  FNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRT 540
            FNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRT
Sbjct: 481  FNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLRT 540

Query: 541  LICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLESL 600
            LICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLESL
Sbjct: 541  LICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLESL 600

Query: 601  SLLYNLQTLKLGQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLVGFE 660
            SLLYNLQTLKLGQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLVGFE
Sbjct: 601  SLLYNLQTLKLGQSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFLVGFE 660

Query: 661  KGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGEDD 720
            KGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGEDD
Sbjct: 661  KGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKRGEDD 720

Query: 721  EDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLP 780
            EDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLP
Sbjct: 721  EDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLP 780

Query: 781  NLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESN 840
            NLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESN
Sbjct: 781  NLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESN 840

Query: 841  LFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKR 900
            LFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKR
Sbjct: 841  LFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKR 900

Query: 901  LPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQHLTN 960
            LPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQHLTN
Sbjct: 901  LPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQLQHLTN 960

Query: 961  LQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIFECP 1020
            LQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIFECP
Sbjct: 961  LQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLDIFECP 1020

Query: 1021 KLLVGEGDQERAKLSHLPSKCVHKSESFYSGLL 1053
            KLLVGEGDQERAKLSHLPSKCVHKSESFYSGLL
Sbjct: 1021 KLLVGEGDQERAKLSHLPSKCVHKSESFYSGLL 1053

BLAST of Cucsat.G12697 vs. ExPASy TrEMBL
Match: A0A0A0KST5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1)

HSP 1 Score: 1362 bits (3525), Expect = 0.0
Identity = 733/1049 (69.88%), Postives = 831/1049 (79.22%), Query Frame = 0

Query: 1    MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60
            MAE + TF VQEVLK+IV +GAEQI +AW LE E+S LK  L  A TIL DIN KKSH  
Sbjct: 1    MAEFLWTFAVQEVLKKIVNFGAEQISLAWGLEKELSHLKKWLLKAQTILADINTKKSHH- 60

Query: 61   NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMA 120
            +SV  WVE+L DI++EADDLLDE+VYE +R+TVE T K  KV DSIS S NSFLF  KMA
Sbjct: 61   HSVGLWVEELHDIIYEADDLLDEIVYEQIRQTVEQTGKLRKVRDSISPSKNSFLFGLKMA 120

Query: 121  KKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELL 180
            KK+K IT TL +HYC AS  GLVG E+ TE E ALNQIRETTSILDF+VEGREAEVLE+L
Sbjct: 121  KKMKKITKTLYEHYCEASPLGLVGDESTTESEAALNQIRETTSILDFEVEGREAEVLEIL 180

Query: 181  KLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKIL 240
            KL IDST+E H+SVISIVGMGGLGKTTLAKM+FNH  I+GHFDKT+WVCVSKPFIV KIL
Sbjct: 181  KLVIDSTDEDHISVISIVGMGGLGKTTLAKMVFNHDAIKGHFDKTVWVCVSKPFIVMKIL 240

Query: 241  EKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIA 300
            E IFQGLT T SGL S +EALL RLR+EMQ K YFLVLDDVWD E  LWDEL G LK+IA
Sbjct: 241  EAIFQGLTNTSSGLNS-REALLNRLREEMQGKKYFLVLDDVWDKENCLWDELIGNLKYIA 300

Query: 301  GKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKK 360
            GK GN+IMVTTR+ EVATMV+ + IY LKKLS+D CWAL K+SANANQL MNSKLE  K 
Sbjct: 301  GKSGNSIMVTTRSVEVATMVKTVPIYHLKKLSDDHCWALLKKSANANQLQMNSKLENTKN 360

Query: 361  ELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILK 420
             LVRK+GGVPL+AKVLGGAVKFEE   E      SWM K+ES  RNIS+EDKDFVLSILK
Sbjct: 361  ILVRKIGGVPLIAKVLGGAVKFEEGGSE------SWMAKIESFARNISIEDKDFVLSILK 420

Query: 421  LSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQ 480
            LSV+SLP+  LKQC AYCSNF QDY+F KD+ I+MWIA+GFIQP Q R+ NL ME+IGE+
Sbjct: 421  LSVESLPHSALKQCFAYCSNFPQDYEFDKDEAIQMWIAEGFIQPEQERE-NLTMENIGEE 480

Query: 481  YFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRKLR 540
            Y NFLLSRS+F+D  +  + RIV FK+HDLMHDIACAIS+H  ++SNP + +GKS RKLR
Sbjct: 481  YLNFLLSRSLFEDAIK-YDGRIVTFKIHDLMHDIACAISNHHKMDSNPISWNGKSTRKLR 540

Query: 541  TLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLES 600
            TLIC +E   +  Q DI+CLRVL + +     L   +DKLIHLRYLDIS C+INKLL +S
Sbjct: 541  TLICENEEAFHKIQTDIICLRVLVLKWFDTNTLSTIMDKLIHLRYLDISNCNINKLLRDS 600

Query: 601  LSLLYNLQTLKLG--QSGLPKNLRKLVNLRHLEFKMFGDTA-MPSDMGNLIHLQSLSGFL 660
            +  LYNLQTLKLG  +  LPKNLR LVNLRHLEFK F D   MPS MGN+IHLQ+LS F+
Sbjct: 601  ICALYNLQTLKLGYIECDLPKNLRNLVNLRHLEFKKFFDMGQMPSHMGNMIHLQTLSEFV 660

Query: 661  VGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNL-WFFETDK 720
            VG EKGCKI+ELGPLK+LKG LTL NL  VQNKDEAMAAKLVEKK LRHL   WF     
Sbjct: 661  VGLEKGCKIDELGPLKDLKGTLTLKNLQNVQNKDEAMAAKLVEKKYLRHLIFQWFLNLYD 720

Query: 721  RGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPM 780
            RGE DED   QVLEGLQPHKN+QSL+I GF+G+VL   IFVENLV+IRL    RCEVLPM
Sbjct: 721  RGEYDEDDNKQVLEGLQPHKNVQSLDIRGFQGRVLNNNIFVENLVEIRLVDCGRCEVLPM 780

Query: 781  LGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATV 840
            LGQLPNLK+LEI+ M SVRSIG+EFYGVD + +NS AFPQL K  I  +  L+QWDEATV
Sbjct: 781  LGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDRNSSAFPQLNKFHICGLKKLQQWDEATV 840

Query: 841  VLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEI 900
               SN FGCLKE+ +  C+ LAKLPSGLEGC+S+EYL+I GC NLMLNVQNL+ LYHL+I
Sbjct: 841  -FASNRFGCLKELILSGCHQLAKLPSGLEGCYSIEYLAIDGCPNLMLNVQNLYNLYHLDI 900

Query: 901  DGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELS--GRYGSVDTQLPQ 960
             GLKRLP     LT LK+L+IGGCMQNYEFS  IHL+SQLVELEL+  G  GS  TQLPQ
Sbjct: 901  RGLKRLPDEFGKLTNLKKLRIGGCMQNYEFSPFIHLSSQLVELELTDDGSSGSETTQLPQ 960

Query: 961  QLQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLEN 1020
            QLQHLTNL+VLKI  FD IE LPEW+GNL  L TL    C  LKELPSREAI RLTKL++
Sbjct: 961  QLQHLTNLKVLKIADFDDIEVLPEWLGNLTCLATLVFLECKNLKELPSREAIQRLTKLDD 1020

Query: 1021 LDIFECPKLLVGEGDQERAKLSHLPSKCV 1042
            L I  CPKLL+GEGDQERAKLSHLPSKCV
Sbjct: 1021 LVIDGCPKLLLGEGDQERAKLSHLPSKCV 1038

BLAST of Cucsat.G12697 vs. ExPASy TrEMBL
Match: A0A6J1CVR6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014685 PE=4 SV=1)

HSP 1 Score: 1095 bits (2833), Expect = 0.0
Identity = 615/1041 (59.08%), Postives = 758/1041 (72.81%), Query Frame = 0

Query: 1    MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60
            MAE + TF VQEVL++IVK  A+QI +AW LE E+S L   L  A+TIL DINR+K H  
Sbjct: 1    MAEFLWTFAVQEVLRKIVKVAAQQIGLAWGLEKELSKLTKWLLKAETILGDINRRKLHH- 60

Query: 61   NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMA 120
            +SV+ W+E L+D+VHEA DLLDELVYE LRR VE   K +KV  SIS S N F FRRKMA
Sbjct: 61   DSVRLWMEDLQDVVHEAHDLLDELVYEDLRRKVE-IGKMNKVRGSISRSYNLFSFRRKMA 120

Query: 121  KKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELL 180
            KK+K++T+TL +HYC AS  GLVG E++   ++ L QIRETTS L+F+V GRE EV  ++
Sbjct: 121  KKMKDVTETLYKHYCEASPLGLVGDESIETNQIVLKQIRETTSNLNFEVVGREIEVSNIV 180

Query: 181  KLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKIL 240
            KL IDS+NEHH S+I IVGMGGLGKTTLA+MIFNH  I+GHFDKTIW+CVS+PFI+  IL
Sbjct: 181  KLVIDSSNEHHTSIIPIVGMGGLGKTTLAQMIFNHEVIKGHFDKTIWICVSEPFIIINIL 240

Query: 241  EKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIA 300
            E IFQGLTKT SGL S KE L+ +L++EM  K YFLVLDDVW+ E  LWD+L  CLK I 
Sbjct: 241  EGIFQGLTKTSSGLNS-KETLVQKLQEEMHGKKYFLVLDDVWNEESGLWDDLGDCLKQIT 300

Query: 301  GKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKK 360
             K GN I+VTTR+ EVAT +E +S + LKKLS+DQ W LFK SANAN L MN KL+I+++
Sbjct: 301  EKSGNCIIVTTRSLEVATTIETVSSHHLKKLSDDQSWFLFKGSANANGLSMNPKLDIIRE 360

Query: 361  ELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILK 420
             LV+K+GGVPLVAKVLGGA+KFE       D+E +W+ KV+S+VRNIS +DKDFVLSILK
Sbjct: 361  ILVKKIGGVPLVAKVLGGAIKFEG------DYE-TWVKKVKSVVRNISKDDKDFVLSILK 420

Query: 421  LSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIGEQ 480
            LSVD LP   LKQC AYCSNFS+D  F K++LI+MWIAQGFIQ  +G ++    EDIGE 
Sbjct: 421  LSVDCLPLLSLKQCFAYCSNFSKDCVFDKENLIQMWIAQGFIQLHEGHNETT-EEDIGEW 480

Query: 481  YFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNV-ESNPNNLSGKSVRKL 540
            + NFLLSRS+FQDV +D N+R+  FKMHDL+HDIACAIS+H  + E   +N S KS RKL
Sbjct: 481  HLNFLLSRSLFQDVVKDMNERVTHFKMHDLIHDIACAISNHPKLLELGSSNWSRKSARKL 540

Query: 541  RTLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLLE 600
            RTLICN++VI Y    D V LRVL +      +L   I KL HLRYLDIS  SI ++L E
Sbjct: 541  RTLICNNQVI-YNELMDCVSLRVLVLNSAKTDNLLDSIGKLKHLRYLDISHYSI-RVLPE 600

Query: 601  SLSLLYNLQTLKLGQ-SGLPKNLRKLVNLRHLEFKM-FGDTAMPSDMGNLIHLQSLSGFL 660
            S++ LYNLQTLK+G+   LPKNLRK+V+LRHL+F   +    MPS M +LIHLQ+L  F 
Sbjct: 601  SIAKLYNLQTLKIGRMESLPKNLRKMVSLRHLKFYHGYHTMQMPSHMSHLIHLQTLYEFA 660

Query: 661  VGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNL-WFFETDK 720
             GFE GCKIEELGPLKNLKG+L L+NL RV++K+EA+AAKLVEKKNL  L   W  + ++
Sbjct: 661  AGFENGCKIEELGPLKNLKGRLRLSNLERVKSKEEAIAAKLVEKKNLHELTFDWSLDFER 720

Query: 721  RGEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLPM 780
              +D E     VLEGLQPHKNLQSL I+ F G+VLP  IFVENL  IRL   ++CE+LPM
Sbjct: 721  NDDDSE-----VLEGLQPHKNLQSLNIMNFGGQVLPEAIFVENLAVIRLCGGKKCEMLPM 780

Query: 781  LGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEATV 840
            LGQLPNL ELEI +M+SVR IG+EFYG  S       F +L+K     + NLEQW EA  
Sbjct: 781  LGQLPNLVELEISFMDSVRCIGSEFYGNGS-------FSKLRKFDFIGLHNLEQWTEAAS 840

Query: 841  VLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLEI 900
            +  SN FG L+ + +  C  L KLP+GLE C SL  L++R C  L LNVQN+H L +L I
Sbjct: 841  I--SNSFGSLQTLNVIGCGNLKKLPNGLEFCSSLHDLTVRDCPKLALNVQNMHNLSNLFI 900

Query: 901  DGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQL 960
             G K LP G+  LT LKE+++ GCMQ+Y+FS  + L S L++L L+     + T+LPQ  
Sbjct: 901  HGFKELPLGLSHLTNLKEMEVVGCMQDYDFSPFLSLQS-LIKLHLND-VSRICTELPQHF 960

Query: 961  QHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENLD 1020
            ++LT L+ L I  FD IEALPEW+ NL SL+TL    C  LK LPSREA+LRLTKLE+L 
Sbjct: 961  ENLTALETLTIVNFDAIEALPEWLENLASLETLNLEDCKNLKRLPSREAMLRLTKLEHLK 1011

Query: 1021 IFECPKLLVGEGDQ-ERAKLS 1035
            +F CP+L  G GD  +RAKLS
Sbjct: 1021 VFRCPQL-PGRGDLLQRAKLS 1011

BLAST of Cucsat.G12697 vs. ExPASy TrEMBL
Match: A0A6J1CU86 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014619 PE=4 SV=1)

HSP 1 Score: 1075 bits (2781), Expect = 0.0
Identity = 606/1033 (58.66%), Postives = 742/1033 (71.83%), Query Frame = 0

Query: 1    MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKKSHPG 60
            MAE + TF VQEVL++IVK  A+QI +A  LE E+S L   L  A+TIL DINRKKSH  
Sbjct: 1    MAEFLWTFAVQEVLRKIVKVAAQQIGLARGLEKELSNLTKWLQKAETILRDINRKKSH-S 60

Query: 61   NSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKMA 120
            +SV+ WVE L+D+VHEADDLLDELVYEHLRR VE   K +KV DS S+S N FLFR KMA
Sbjct: 61   DSVRLWVEDLQDVVHEADDLLDELVYEHLRRKVE-IGKINKVRDSFSTSHNVFLFRLKMA 120

Query: 121  KKIKNITDTLNQHYCAASAFGLVGVETVTEIELALNQIRETTSILDFQVEGREAEVLELL 180
            KKIK+IT  L++HYC AS  GLVG E+    ++ L+QIR+TTS L+F+V GRE EV  ++
Sbjct: 121  KKIKDITQILHKHYCEASPLGLVGQESTEANQIVLSQIRDTTSNLNFEVVGREDEVSNIV 180

Query: 181  KLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTKIL 240
            KL IDS+NEHHMS++ IVGMGGLGKTT A+ IFNH  I+GHFD  IWVCVS+PFI+  IL
Sbjct: 181  KLVIDSSNEHHMSILPIVGMGGLGKTTFAQKIFNHDTIKGHFDNIIWVCVSEPFIIINIL 240

Query: 241  EKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKHIA 300
            E IFQ LTKT SGL ++K  LL +++KEM  K YFLVLDDVW+ E  LWD+L  CLK IA
Sbjct: 241  EGIFQSLTKTSSGL-NHKGVLLEKIQKEMHGKMYFLVLDDVWNEESGLWDDLGDCLKQIA 300

Query: 301  GKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIMKK 360
            GK GN I+VTTR+ EVA +V  +  +RLKKLS+DQCW LFKESANAN+L MN KLEI++ 
Sbjct: 301  GKSGNCIIVTTRSLEVANIVGTVPSHRLKKLSDDQCWFLFKESANANRLSMNPKLEIIRD 360

Query: 361  ELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSILK 420
             LV+K+GGVPLVAKVLGGA KFE       D+E  W+TKVES+VRNIS +DKDFV SILK
Sbjct: 361  MLVKKIGGVPLVAKVLGGATKFEG------DYE-RWVTKVESVVRNISNDDKDFVSSILK 420

Query: 421  LSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQP-GQGRDKNLLMEDIGE 480
            LSVD LP   LKQC AYCSNF++D  + K++LI++WIAQGFIQP  Q  D  +  EDIGE
Sbjct: 421  LSVDCLPLLSLKQCFAYCSNFAKDCLYDKENLIRIWIAQGFIQPQDQEGDNEMTEEDIGE 480

Query: 481  QYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNV--ESNPNNLSGKSVR 540
             +FNFLLSRS+FQDVT D ++R + F MHDL+HDIACAIS+HQ +  E + +N S KS R
Sbjct: 481  WHFNFLLSRSLFQDVTMDKSERGIYFIMHDLIHDIACAISNHQKLLHELSSSNRSRKSAR 540

Query: 541  KLRTLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLL 600
            KLRTLICN++VI Y    D V LRVL +  +   +L   I KL HLRYL I+  +   +L
Sbjct: 541  KLRTLICNNQVI-YNELVDCVSLRVLVLHCEKTGNLLESIGKLKHLRYLSIANGNYVDVL 600

Query: 601  LESLSLLYNLQTLKLGQ-SGLPKNLRKLVNLRHLEFKMFGD-TAMPSDMGNLIHLQSLSG 660
             ES+ +LYNLQTLKL     LPKN RK+V+LRHLEF++  D   MPS M  LIHLQ+LS 
Sbjct: 601  PESIVMLYNLQTLKLRHMKSLPKNSRKMVSLRHLEFELKSDFRQMPSHMSELIHLQTLSR 660

Query: 661  FLVGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETD 720
            F+ GFE GCKIEELGPLKNLKGKL L+ L RV++K+E++ AKLVEKKNL+ L    FE  
Sbjct: 661  FVAGFENGCKIEELGPLKNLKGKLQLSCLERVESKEESIVAKLVEKKNLQEL---IFEWS 720

Query: 721  KR--GEDDEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEV 780
             R  G++     ++VLEGLQPHKNLQSL ++GF G+VL   IFVENL  IRL + + CE 
Sbjct: 721  SRFEGKNSRYSELEVLEGLQPHKNLQSLTVIGFGGQVLGDAIFVENLTVIRLSNCKNCER 780

Query: 781  LPMLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDE 840
            LPMLG+LPNL EL I  M+S+R IG+EFYG  S++Q   +F +L+KL+     NLEQW +
Sbjct: 781  LPMLGRLPNLVELNINSMDSLRCIGSEFYGNGSNNQGH-SFSKLRKLTFIWCRNLEQWKD 840

Query: 841  ATVVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYH 900
            A VV  SN FG L+ + I  C  L KLP+GLEGC SL  L++  C  L LNVQN   L +
Sbjct: 841  AAVV--SNSFGSLQTLSIYGCGNLEKLPNGLEGCSSLRDLTVAYCPKLSLNVQNCQNLSN 900

Query: 901  LEIDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLP 960
            L IDGLK+LP G+  LT LKE+K  GCMQ+Y+F+  + L S +++LEL        TQLP
Sbjct: 901  LRIDGLKKLPLGLGHLTNLKEMKFVGCMQDYDFTPFVSLQSLIIKLELRDECSGNCTQLP 960

Query: 961  QQLQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLE 1020
              LQHLT L+ L I  F  IE  PEW+ NL SL+ L    C  L+ LPS EAI RLTKLE
Sbjct: 961  PHLQHLTALKQLSIGNFGGIEVPPEWLNNLTSLEMLSFEGCENLRRLPSTEAIRRLTKLE 1016

Query: 1021 NLDIFECPKLLVG 1025
             L   +CP L  G
Sbjct: 1021 LLSAIKCPLLYGG 1016

BLAST of Cucsat.G12697 vs. ExPASy TrEMBL
Match: A0A6J1CUL8 (putative disease resistance protein RGA4 OS=Momordica charantia OX=3673 GN=LOC111014734 PE=4 SV=1)

HSP 1 Score: 980 bits (2533), Expect = 0.0
Identity = 564/1044 (54.02%), Postives = 725/1044 (69.44%), Query Frame = 0

Query: 1    MAE-IGTFVVQEVLKRIVKYGAEQIVVAWELENEVSLLKDKLHDADTILEDINRKK-SHP 60
            MAE + TF VQEVLK+++K  AEQI VAW L+ E+S L+ +L  A+TIL DINR+K  H 
Sbjct: 1    MAEFLWTFAVQEVLKKVLKLAAEQIGVAWALDKELSKLRKRLLKAETILGDINRRKLCH- 60

Query: 61   GNSVKRWVEKLEDIVHEADDLLDELVYEHLRRTVEHTEKFSKVSDSISSSINSFLFRRKM 120
              SV+ WVE L+ +V++ADDLLD+LVYE LR+ VE  EK +KV + +S S N FLFR KM
Sbjct: 61   -ESVRLWVEDLQHLVYQADDLLDDLVYEDLRQKVE-MEKINKVRNFLSPSNNPFLFRFKM 120

Query: 121  AKKIKNITDTLNQHYCAASAFGLVGVE-TVTEIELALNQIRETTSILDFQVEGREAEVLE 180
            AK++K I++TL +HYC AS  G VG E T T  +  L QIRETTSIL+F+V GRE EVL+
Sbjct: 121  AKRMKAISETLYEHYCEASVLGFVGEESTETTYKNILRQIRETTSILNFKVIGREIEVLK 180

Query: 181  LLKLAIDSTNEHHMSVISIVGMGGLGKTTLAKMIFNHREIEGHFDKTIWVCVSKPFIVTK 240
            ++KL IDS++EH MS+I IVGMGGLGKTTLAKM+FNH  I+ HFDKTIWVCVSKPFI+  
Sbjct: 181  IMKLVIDSSHEHRMSIIPIVGMGGLGKTTLAKMVFNHELIKEHFDKTIWVCVSKPFIIVN 240

Query: 241  ILEKIFQGLTKTCSGLESNKEALLGRLRKEMQDKNYFLVLDDVWDNEKHLWDELRGCLKH 300
            ILE IFQ LT T SGL + KE L  RL+KEMQ K YFLVLDDVW+ E  LWDELR CL  
Sbjct: 241  ILEGIFQSLTNTSSGL-NYKETLFHRLQKEMQGKKYFLVLDDVWNEEVALWDELRDCLNE 300

Query: 301  IAGKPGNTIMVTTRNEEVATMVEPISIYRLKKLSNDQCWALFKESANANQLPMNSKLEIM 360
            IA K GN+I+VTTR+  VAT+++ +  +RL+KLS+D+CW+LFKESANAN L MN +LE +
Sbjct: 301  IAEKSGNSIIVTTRSTVVATIMQTVPSHRLRKLSDDECWSLFKESANANGLLMNPELENI 360

Query: 361  KKELVRKMGGVPLVAKVLGGAVKFEETELEEEDHEISWMTKVESIVRNISLEDKDFVLSI 420
            ++ +V+++GG+PLVAKVLGG  KFE            W+T VESIVRNI  ++KDFVLS 
Sbjct: 361  REVVVKRIGGIPLVAKVLGGTAKFEGNYER-------WVTTVESIVRNILNDEKDFVLST 420

Query: 421  LKLSVDSLPNPVLKQCVAYCSNFSQDYDFQKDDLIKMWIAQGFIQPGQGRDKNLLMEDIG 480
            LKLSVDSLP   LKQC A+CSNF +D +F K++LI+MWIAQGFIQ  +G D  +L E+IG
Sbjct: 421  LKLSVDSLPLLSLKQCFAFCSNFPKDCEFVKENLIQMWIAQGFIQLEEG-DNVVLEENIG 480

Query: 481  EQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDIACAISSHQNVESNPNNLSGKSVRK 540
            E YFNFLLSRS+FQDV++D +  I  FKMHDL+HD+AC +S+ Q +E +P N S K VR 
Sbjct: 481  EGYFNFLLSRSLFQDVSKDIDGTITHFKMHDLIHDVACIVSN-QKLELDPRNWSEKGVRA 540

Query: 541  LRTLICNDEVINYLNQNDIVCLRVLKVIFQSHTDLWIPIDKLIHLRYLDISECSINKLLL 600
            LRTL+ N+  I +    D V LRVL +      +L   I  L HLRYLDI    I +L  
Sbjct: 541  LRTLVINNGAIIHNKLEDSVYLRVLVLNSDFIKNLPDSIGMLKHLRYLDIIGSRIRRLP- 600

Query: 601  ESLSLLYNLQTLKLG-QSGLPKNLRKLVNLRHLEFKMFGDTAMPSDMGNLIHLQSLSGFL 660
            ES+++LYNLQ LKL  +  LPKNLRKLV+LRHL F       MP  +  LIHLQ+L  ++
Sbjct: 601  ESITMLYNLQILKLRTRIDLPKNLRKLVSLRHLPFFYIHMEKMPLHVSQLIHLQTLPLYI 660

Query: 661  VGFEKGCKIEELGPLKNLKGKLTLTNLWRVQNKDEAMAAKLVEKKNLRHLNLWFFETDKR 720
            VGFEKG KIEELGPL +LKG L L NL RV++K+EA+AAKL+EK NL  L L  + +   
Sbjct: 661  VGFEKGRKIEELGPLNHLKGSLRLLNLERVKSKEEAIAAKLIEKNNLCELTL-HWNSSSC 720

Query: 721  GED--DEDGIVQVLEGLQPHKNLQSLEILGFRGKVLPTGIFVENLVKIRLGHFERCEVLP 780
            GE+  +     +VLEGLQPH+NLQSL I  F G  LP  +FVENLV I L    +CE+LP
Sbjct: 721  GEEGYNNRNDSEVLEGLQPHRNLQSLSIFHFGGHALPNVVFVENLVAIVLQECNKCEMLP 780

Query: 781  MLGQLPNLKELEIMYMESVRSIGNEFYGVDSSHQNSVAFPQLKKLSIYEMMNLEQWDEAT 840
            MLGQLPNL+EL + +M SVR IG+EFYG +S+ +  + FP+L++LS   M  LEQW+E +
Sbjct: 781  MLGQLPNLEELRVSFMGSVRIIGSEFYGNNSNSREQIFFPKLRQLSFSHMEKLEQWEELS 840

Query: 841  VVLESNLFGCLKEVRIRRCNPLAKLPSGLEGCHSLEYLSIRGCFNLMLNVQNLHKLYHLE 900
              +    F  L+ + +  C+ L  +P       SL  L I GC  L      L     ++
Sbjct: 841  NGIN---FPHLETLSMFYCSKLTSVPD--HEFVSLRTLRIVGCEELSKLPSKLECCNFMK 900

Query: 901  IDGLKRLPKGMDGLTRLKELKIGGCMQNYEFSSVIHLASQLVELELSGRYGSVDTQLPQQ 960
            I G  +L   M  +  L EL+I G M+ Y+FS ++H +S +  + L+    +  TQLPQQ
Sbjct: 901  ISGCPKLRLNMHNMCNLNELEINGPMEEYDFSFLMHQSS-VTNIFLTDSLRNA-TQLPQQ 960

Query: 961  LQHLTNLQVLKITQFDCIEALPEWIGNLISLKTLKCSYCFKLKELPSREAILRLTKLENL 1020
            LQHLT L+ L I  F+ +EALPEW+  L +L+T+    C  LK LPS++A+LRLTKL++L
Sbjct: 961  LQHLTTLKSLAIEYFNGVEALPEWLEKLANLETMGVFECKNLKRLPSQQAMLRLTKLKHL 1020

Query: 1021 DIFECPKLLVGEGDQERAKLSHLP 1038
             +  CP L +G+GD ERAKLSHLP
Sbjct: 1021 RVRGCPLLPLGQGDLERAKLSHLP 1022